JP2018529326A5 - - Google Patents
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- JP2018529326A5 JP2018529326A5 JP2018508166A JP2018508166A JP2018529326A5 JP 2018529326 A5 JP2018529326 A5 JP 2018529326A5 JP 2018508166 A JP2018508166 A JP 2018508166A JP 2018508166 A JP2018508166 A JP 2018508166A JP 2018529326 A5 JP2018529326 A5 JP 2018529326A5
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- Prior art keywords
- nickase
- nucleic acid
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- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 182
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 150
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 150
- 150000007523 nucleic acids Chemical group 0.000 claims description 108
- 239000000523 sample Substances 0.000 claims description 82
- 108020004414 DNA Proteins 0.000 claims description 57
- 101710163270 Nuclease Proteins 0.000 claims description 53
- 102000039446 nucleic acids Human genes 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 44
- 125000003729 nucleotide group Chemical group 0.000 claims description 33
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 27
- 102000053602 DNA Human genes 0.000 claims description 22
- 239000002773 nucleotide Substances 0.000 claims description 21
- 108020005004 Guide RNA Proteins 0.000 claims description 16
- 230000008685 targeting Effects 0.000 claims description 16
- 108091033409 CRISPR Proteins 0.000 claims description 15
- 108091092584 GDNA Proteins 0.000 claims description 15
- 108091092878 Microsatellite Proteins 0.000 claims description 14
- 230000000295 complement effect Effects 0.000 claims description 12
- 102000004190 Enzymes Human genes 0.000 claims description 10
- 108090000790 Enzymes Proteins 0.000 claims description 10
- 239000012472 biological sample Substances 0.000 claims description 10
- 230000007613 environmental effect Effects 0.000 claims description 10
- 101150069031 CSN2 gene Proteins 0.000 claims description 9
- 101150055601 cops2 gene Proteins 0.000 claims description 9
- 206010064571 Gene mutation Diseases 0.000 claims description 7
- 108700020796 Oncogene Proteins 0.000 claims description 7
- 230000037430 deletion Effects 0.000 claims description 7
- 238000012217 deletion Methods 0.000 claims description 7
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 108010017826 DNA Polymerase I Proteins 0.000 claims description 6
- 102000004594 DNA Polymerase I Human genes 0.000 claims description 6
- 230000000977 initiatory effect Effects 0.000 claims description 6
- 230000005782 double-strand break Effects 0.000 claims description 5
- 238000001668 nucleic acid synthesis Methods 0.000 claims description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 3
- 108090000364 Ligases Proteins 0.000 claims description 3
- 102000003960 Ligases Human genes 0.000 claims description 3
- 108010006785 Taq Polymerase Proteins 0.000 claims description 3
- 230000006820 DNA synthesis Effects 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 description 29
- 230000003197 catalytic effect Effects 0.000 description 26
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
- 239000012634 fragment Substances 0.000 description 15
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 238000012163 sequencing technique Methods 0.000 description 7
- 238000010354 CRISPR gene editing Methods 0.000 description 6
- 102000008579 Transposases Human genes 0.000 description 6
- 108010020764 Transposases Proteins 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 108020004682 Single-Stranded DNA Proteins 0.000 description 4
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- 102000004533 Endonucleases Human genes 0.000 description 3
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- 238000010367 cloning Methods 0.000 description 3
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- 241000282693 Cercopithecidae Species 0.000 description 1
- 101100329224 Coprinopsis cinerea (strain Okayama-7 / 130 / ATCC MYA-4618 / FGSC 9003) cpf1 gene Proteins 0.000 description 1
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Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021193224A JP2022046466A (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562207359P | 2015-08-19 | 2015-08-19 | |
| US62/207,359 | 2015-08-19 | ||
| PCT/US2016/047631 WO2017031360A1 (en) | 2015-08-19 | 2016-08-18 | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021193224A Division JP2022046466A (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018529326A JP2018529326A (ja) | 2018-10-11 |
| JP2018529326A5 true JP2018529326A5 (enExample) | 2019-09-26 |
| JP6986509B2 JP6986509B2 (ja) | 2021-12-22 |
Family
ID=58050956
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| JP2021193224A Pending JP2022046466A (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021193224A Pending JP2022046466A (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10538758B2 (enExample) |
| EP (2) | EP3985111A1 (enExample) |
| JP (2) | JP6986509B2 (enExample) |
| CN (1) | CN108138176B (enExample) |
| AU (1) | AU2016308283B2 (enExample) |
| CA (1) | CA2995983A1 (enExample) |
| DK (1) | DK3337898T3 (enExample) |
| WO (1) | WO2017031360A1 (enExample) |
Families Citing this family (59)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| JP6416939B2 (ja) | 2014-02-13 | 2018-10-31 | タカラ バイオ ユーエスエー，インコーポレイティド | 核酸の初期収集物から標的分子を減損させる方法、並びにそれを実施するための組成物及びキット |
| EP4079847A1 (en) | 2014-07-30 | 2022-10-26 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CN107406875B (zh) * | 2014-12-20 | 2021-12-07 | 阿克生物公司 | 使用CRISPR/Cas系统蛋白靶向消减、富集、和分割核酸的组合物及方法 |
| WO2017031360A1 (en) | 2015-08-19 | 2017-02-23 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| IL294014B2 (en) | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and uses thereof |
| CN109310784B (zh) | 2015-12-07 | 2022-08-19 | 阿克生物公司 | 用于制备和使用指导核酸的方法和组合物 |
| WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
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| EP3638792A1 (en) * | 2017-06-14 | 2020-04-22 | Wisconsin Alumni Research Foundation | Modified guide rnas, crispr-ribonucleotprotein complexes and methods of use |
| CN107190008A (zh) * | 2017-07-19 | 2017-09-22 | 苏州吉赛基因测序科技有限公司 | 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用 |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| CA3077086A1 (en) * | 2017-10-04 | 2019-04-11 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
| WO2019079347A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | USES OF BASIC EDITORS ADENOSINE |
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| US20190083656A1 (en) | 2015-10-16 | 2019-03-21 | Temple University - Of The Commonwealth System Of Higher Education | Methods and compositions utilizing cpf1 for rna-guided gene editing |
| CN109312400A (zh) | 2016-03-25 | 2019-02-05 | 凯锐思公司 | 合成核酸掺入物 |
| WO2018035062A1 (en) | 2016-08-16 | 2018-02-22 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (flash) |
| US20180051320A1 (en) | 2016-08-22 | 2018-02-22 | The Regents Of The University Of California | Depletion of abundant sequences by hybridization (dash) |
| WO2018069430A1 (en) | 2016-10-13 | 2018-04-19 | bioMérieux | Identification and antibiotic characterization of pathogens in metagenomic sample |
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2016
- 2016-08-18 WO PCT/US2016/047631 patent/WO2017031360A1/en not_active Ceased
- 2016-08-18 US US15/520,052 patent/US10538758B2/en active Active
- 2016-08-18 EP EP21188035.6A patent/EP3985111A1/en active Pending
- 2016-08-18 CN CN201680057464.3A patent/CN108138176B/zh active Active
- 2016-08-18 DK DK16837863.6T patent/DK3337898T3/da active
- 2016-08-18 AU AU2016308283A patent/AU2016308283B2/en active Active
- 2016-08-18 CA CA2995983A patent/CA2995983A1/en active Pending
- 2016-08-18 EP EP16837863.6A patent/EP3337898B1/en active Active
- 2016-08-18 JP JP2018508166A patent/JP6986509B2/ja active Active
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2019
- 2019-12-02 US US16/700,493 patent/US20200087652A1/en not_active Abandoned
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2021
- 2021-11-29 JP JP2021193224A patent/JP2022046466A/ja active Pending
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2023
- 2023-02-01 US US18/163,196 patent/US20240132872A1/en active Pending
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