EP3638809A4 - Negative-positive enrichment for nucleic acid detection - Google Patents

Negative-positive enrichment for nucleic acid detection Download PDF

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Publication number
EP3638809A4
EP3638809A4 EP18817717.4A EP18817717A EP3638809A4 EP 3638809 A4 EP3638809 A4 EP 3638809A4 EP 18817717 A EP18817717 A EP 18817717A EP 3638809 A4 EP3638809 A4 EP 3638809A4
Authority
EP
European Patent Office
Prior art keywords
negative
nucleic acid
acid detection
positive enrichment
enrichment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18817717.4A
Other languages
German (de)
French (fr)
Other versions
EP3638809A1 (en
Inventor
Anthony P. Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Research LLC
Original Assignee
Genetics Research LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/877,619 external-priority patent/US10527608B2/en
Application filed by Genetics Research LLC filed Critical Genetics Research LLC
Publication of EP3638809A1 publication Critical patent/EP3638809A1/en
Publication of EP3638809A4 publication Critical patent/EP3638809A4/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
EP18817717.4A 2017-06-13 2018-06-13 Negative-positive enrichment for nucleic acid detection Withdrawn EP3638809A4 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201762519051P 2017-06-13 2017-06-13
US201762526091P 2017-06-28 2017-06-28
US201762568121P 2017-10-04 2017-10-04
US15/877,619 US10527608B2 (en) 2017-06-13 2018-01-23 Methods for rare event detection
US201862656592P 2018-04-12 2018-04-12
PCT/US2018/037277 WO2018231945A1 (en) 2017-06-13 2018-06-13 Negative-positive enrichment for nucleic acid detection

Publications (2)

Publication Number Publication Date
EP3638809A1 EP3638809A1 (en) 2020-04-22
EP3638809A4 true EP3638809A4 (en) 2021-03-10

Family

ID=64659281

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18817717.4A Withdrawn EP3638809A4 (en) 2017-06-13 2018-06-13 Negative-positive enrichment for nucleic acid detection

Country Status (3)

Country Link
EP (1) EP3638809A4 (en)
CA (2) CA3069934A1 (en)
WO (1) WO2018231945A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
WO2019030306A1 (en) 2017-08-08 2019-02-14 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
US20210040537A1 (en) * 2019-08-08 2021-02-11 Genetics Research, Llc Selective enrichment

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016028887A1 (en) * 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
WO2017031360A1 (en) * 2015-08-19 2017-02-23 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
EP3150718A1 (en) * 2014-05-28 2017-04-05 Toolgen Incorporated Method for sensitive detection of target dna using target-specific nuclease
WO2019030306A1 (en) * 2017-08-08 2019-02-14 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
WO2019178577A1 (en) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011075774A1 (en) * 2009-12-23 2011-06-30 Genetic Technologies Limited Methods of enriching and detecting fetal nucleic acids
US10421993B2 (en) * 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
US10815522B2 (en) * 2015-02-20 2020-10-27 The Johns Hopkins University Genomic alterations in the tumor and circulation of pancreatic cancer patients
ES2788176T3 (en) * 2015-09-24 2020-10-20 Sigma Aldrich Co Llc Methods and reagents for the detection of molecular proximity using RNA-guided nucleic acid-binding proteins

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3150718A1 (en) * 2014-05-28 2017-04-05 Toolgen Incorporated Method for sensitive detection of target dna using target-specific nuclease
WO2016028887A1 (en) * 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
WO2017031360A1 (en) * 2015-08-19 2017-02-23 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
WO2019030306A1 (en) * 2017-08-08 2019-02-14 Depixus In vitro isolation and enrichment of nucleic acids using site-specific nucleases
WO2019178577A1 (en) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JENNIFER L. STEELE ET AL: "Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations", PLOS ONE, vol. 15, no. 12, 23 December 2020 (2020-12-23), pages e0243781, XP055768725, DOI: 10.1371/journal.pone.0243781 *
RICHARD C. STEVENS ET AL: "A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment", PLOS ONE, vol. 14, no. 4, 18 April 2019 (2019-04-18), pages e0215441, XP055751103, DOI: 10.1371/journal.pone.0215441 *
See also references of WO2018231945A1 *

Also Published As

Publication number Publication date
EP3638809A1 (en) 2020-04-22
WO2018231945A1 (en) 2018-12-20
CA3069934A1 (en) 2018-12-20
CA3222142A1 (en) 2018-12-20

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