EP3638809A4 - Enrichissement négatif-positif pour la détection d'acides nucléiques - Google Patents

Enrichissement négatif-positif pour la détection d'acides nucléiques Download PDF

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Publication number
EP3638809A4
EP3638809A4 EP18817717.4A EP18817717A EP3638809A4 EP 3638809 A4 EP3638809 A4 EP 3638809A4 EP 18817717 A EP18817717 A EP 18817717A EP 3638809 A4 EP3638809 A4 EP 3638809A4
Authority
EP
European Patent Office
Prior art keywords
negative
nucleic acid
acid detection
positive enrichment
enrichment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP18817717.4A
Other languages
German (de)
English (en)
Other versions
EP3638809A1 (fr
Inventor
Anthony P. Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Research LLC
Original Assignee
Genetics Research LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US15/877,619 external-priority patent/US10527608B2/en
Application filed by Genetics Research LLC filed Critical Genetics Research LLC
Publication of EP3638809A1 publication Critical patent/EP3638809A1/fr
Publication of EP3638809A4 publication Critical patent/EP3638809A4/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
EP18817717.4A 2017-06-13 2018-06-13 Enrichissement négatif-positif pour la détection d'acides nucléiques Withdrawn EP3638809A4 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201762519051P 2017-06-13 2017-06-13
US201762526091P 2017-06-28 2017-06-28
US201762568121P 2017-10-04 2017-10-04
US15/877,619 US10527608B2 (en) 2017-06-13 2018-01-23 Methods for rare event detection
US201862656592P 2018-04-12 2018-04-12
PCT/US2018/037277 WO2018231945A1 (fr) 2017-06-13 2018-06-13 Enrichissement négatif-positif pour la détection d'acides nucléiques

Publications (2)

Publication Number Publication Date
EP3638809A1 EP3638809A1 (fr) 2020-04-22
EP3638809A4 true EP3638809A4 (fr) 2021-03-10

Family

ID=64659281

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18817717.4A Withdrawn EP3638809A4 (fr) 2017-06-13 2018-06-13 Enrichissement négatif-positif pour la détection d'acides nucléiques

Country Status (3)

Country Link
EP (1) EP3638809A4 (fr)
CA (2) CA3069934A1 (fr)
WO (1) WO2018231945A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
WO2019030306A1 (fr) 2017-08-08 2019-02-14 Depixus Isolement et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site
CA3150283A1 (fr) * 2019-08-08 2021-02-11 Genetics Research, Llc Enrichissement selectif

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016028887A1 (fr) * 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions et méthodes pour l'enrichissement d'acides nucléiques
WO2017031360A1 (fr) * 2015-08-19 2017-02-23 Arc Bio, Llc Capture d'acides nucléiques à l'aide d'un système utilisant une nucléase guidée par des acides nucléiques
EP3150718A1 (fr) * 2014-05-28 2017-04-05 Toolgen Incorporated Procédé permettant la détection sensible d'adn cible à l'aide d'une nucléase spécifique de cible
WO2019030306A1 (fr) * 2017-08-08 2019-02-14 Depixus Isolement et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site
WO2019178577A1 (fr) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Procédés et réactifs pour l'enrichissement de matériau d'acide nucléique pour des applications de séquençage et d'autres interrogations de matériau d'acide nucléique

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120315633A1 (en) * 2009-12-23 2012-12-13 Genetic Technologies Limited Methods of enriching and detecting fetal nucleic acids
US10421993B2 (en) * 2015-02-11 2019-09-24 Paragon Genomics, Inc. Methods and compositions for reducing non-specific amplification products
WO2016134136A2 (fr) * 2015-02-20 2016-08-25 The Johns Hopkins University Altérations génomiques dans la tumeur et circulation de patients atteints d'un cancer du pancréas
JP2018531596A (ja) * 2015-09-24 2018-11-01 シグマ−アルドリッチ・カンパニー・リミテッド・ライアビリティ・カンパニーＳｉｇｍａ−Ａｌｄｒｉｃｈ Ｃｏ．， ＬＬＣ Ｒｎａガイド型核酸結合タンパク質を使用する分子間近接性検出用の方法および試薬

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3150718A1 (fr) * 2014-05-28 2017-04-05 Toolgen Incorporated Procédé permettant la détection sensible d'adn cible à l'aide d'une nucléase spécifique de cible
WO2016028887A1 (fr) * 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions et méthodes pour l'enrichissement d'acides nucléiques
WO2017031360A1 (fr) * 2015-08-19 2017-02-23 Arc Bio, Llc Capture d'acides nucléiques à l'aide d'un système utilisant une nucléase guidée par des acides nucléiques
WO2019030306A1 (fr) * 2017-08-08 2019-02-14 Depixus Isolement et enrichissement in vitro d'acides nucléiques à l'aide de nucléases spécifiques à un site
WO2019178577A1 (fr) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Procédés et réactifs pour l'enrichissement de matériau d'acide nucléique pour des applications de séquençage et d'autres interrogations de matériau d'acide nucléique

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JENNIFER L. STEELE ET AL: "Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations", PLOS ONE, vol. 15, no. 12, 23 December 2020 (2020-12-23), pages e0243781, XP055768725, DOI: 10.1371/journal.pone.0243781 *
RICHARD C. STEVENS ET AL: "A novel CRISPR/Cas9 associated technology for sequence-specific nucleic acid enrichment", PLOS ONE, vol. 14, no. 4, 18 April 2019 (2019-04-18), pages e0215441, XP055751103, DOI: 10.1371/journal.pone.0215441 *
See also references of WO2018231945A1 *

Also Published As

Publication number Publication date
EP3638809A1 (fr) 2020-04-22
CA3222142A1 (fr) 2018-12-20
CA3069934A1 (fr) 2018-12-20
WO2018231945A1 (fr) 2018-12-20

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