JP2020527938A5 - - Google Patents
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- JP2020527938A5 JP2020527938A5 JP2019568653A JP2019568653A JP2020527938A5 JP 2020527938 A5 JP2020527938 A5 JP 2020527938A5 JP 2019568653 A JP2019568653 A JP 2019568653A JP 2019568653 A JP2019568653 A JP 2019568653A JP 2020527938 A5 JP2020527938 A5 JP 2020527938A5
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- 108090000790 Enzymes Proteins 0.000 description 30
- 102000004190 Enzymes Human genes 0.000 description 30
- 239000002773 nucleotide Substances 0.000 description 26
- 125000003729 nucleotide group Chemical group 0.000 description 26
- 108020004414 DNA Proteins 0.000 description 24
- 238000000034 method Methods 0.000 description 19
- 125000006850 spacer group Chemical group 0.000 description 14
- 238000012270 DNA recombination Methods 0.000 description 13
- 239000013598 vector Substances 0.000 description 13
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 108020004707 nucleic acids Proteins 0.000 description 10
- 102000039446 nucleic acids Human genes 0.000 description 10
- 230000001939 inductive effect Effects 0.000 description 5
- 230000037430 deletion Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 231100000221 frame shift mutation induction Toxicity 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000008194 pharmaceutical composition Substances 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2023092153A JP2023116580A (ja) | 2017-06-14 | 2023-06-05 | デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17175895 | 2017-06-14 | ||
| EP17175895.6 | 2017-06-14 | ||
| PCT/EP2018/065881 WO2018229226A1 (en) | 2017-06-14 | 2018-06-14 | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023092153A Division JP2023116580A (ja) | 2017-06-14 | 2023-06-05 | デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020527938A JP2020527938A (ja) | 2020-09-17 |
| JP2020527938A5 true JP2020527938A5 (enExample) | 2021-07-26 |
| JP7402503B2 JP7402503B2 (ja) | 2023-12-21 |
Family
ID=59269741
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019568653A Active JP7402503B2 (ja) | 2017-06-14 | 2018-06-14 | デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 |
| JP2023092153A Pending JP2023116580A (ja) | 2017-06-14 | 2023-06-05 | デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023092153A Pending JP2023116580A (ja) | 2017-06-14 | 2023-06-05 | デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11845954B2 (enExample) |
| EP (1) | EP3638776B1 (enExample) |
| JP (2) | JP7402503B2 (enExample) |
| CN (1) | CN110914414A (enExample) |
| CA (1) | CA3066047A1 (enExample) |
| DK (1) | DK3638776T3 (enExample) |
| FI (1) | FI3638776T3 (enExample) |
| WO (1) | WO2018229226A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018229226A1 (en) | 2017-06-14 | 2018-12-20 | Technische Universität Dresden | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
| CN115066255A (zh) | 2019-09-18 | 2022-09-16 | 星际治疗有限公司 | 合成的dna载体和使用方法 |
| EP3831939A1 (en) | 2019-12-06 | 2021-06-09 | Technische Universität Dresden | Fusion of site-specific recombinases for efficient and specific genome editing |
| MX2024005779A (es) | 2021-11-15 | 2024-05-24 | Univ Dresden Tech | Recombinasas especificas de sitio para edicion eficiente y especifica del genoma. |
| WO2025143215A1 (ja) * | 2023-12-28 | 2025-07-03 | デンカ株式会社 | 多型塩基を組み込んだ標的配列を作製するための組換えベクター |
| WO2025252950A1 (en) | 2024-06-07 | 2025-12-11 | Technische Universität Dresden Körperschaft Des Öffentlichen Rechts | Method for producing spacer-specific dna recombining enzymes |
Family Cites Families (46)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1293460C (en) | 1985-10-07 | 1991-12-24 | Brian Lee Sauer | Site-specific recombination of dna in yeast |
| US4861719A (en) | 1986-04-25 | 1989-08-29 | Fred Hutchinson Cancer Research Center | DNA constructs for retrovirus packaging cell lines |
| US5278056A (en) | 1988-02-05 | 1994-01-11 | The Trustees Of Columbia University In The City Of New York | Retroviral packaging cell lines and process of using same |
| US5670488A (en) | 1992-12-03 | 1997-09-23 | Genzyme Corporation | Adenovirus vector for gene therapy |
| DE69434860T2 (de) | 1993-02-22 | 2007-03-15 | The Rockefeller University | Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion |
| FR2712812B1 (fr) | 1993-11-23 | 1996-02-09 | Centre Nat Rech Scient | Composition pour la production de produits thérapeutiques in vivo. |
| IL116816A (en) | 1995-01-20 | 2003-05-29 | Rhone Poulenc Rorer Sa | Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof |
| US6013516A (en) | 1995-10-06 | 2000-01-11 | The Salk Institute For Biological Studies | Vector and method of use for nucleic acid delivery to non-dividing cells |
| US6774279B2 (en) | 1997-05-30 | 2004-08-10 | Carnegie Institution Of Washington | Use of FLP recombinase in mice |
| US5981830A (en) | 1997-05-30 | 1999-11-09 | Schering Aktiengesellschaft | Knockout mice and their progeny with a disrupted hepsin gene |
| US5929301A (en) | 1997-11-18 | 1999-07-27 | Pioneer Hi-Bred International | Nucleic acid sequence encoding FLP recombinase |
| PT1032680E (pt) | 1997-11-18 | 2006-05-31 | Pioneer Hi Bred Int | Um novo metodo para a integracao de adn estranho em genomas eucarioticos |
| ES2245487T3 (es) | 1997-11-18 | 2006-01-01 | Pioneer Hi-Bred International, Inc. | Composiciones y metodos para la modificacion genetica de plantas. |
| DE19941186A1 (de) | 1999-08-30 | 2001-03-01 | Peter Droege | Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen |
| GB0029375D0 (en) | 2000-12-01 | 2001-01-17 | Total Fina Elf | Substrate linked directed evolution (slide) |
| DE10140030C1 (de) | 2001-08-16 | 2002-12-19 | Gsf Forschungszentrum Umwelt | Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System |
| WO2005081632A2 (en) | 2004-02-26 | 2005-09-09 | State Of Israel, Ministry Of Agriculture, Agricultural Research Organization | Enzymes, cells and methods for site specific recombination at asymmetric sites |
| US7422889B2 (en) | 2004-10-29 | 2008-09-09 | Stowers Institute For Medical Research | Dre recombinase and recombinase systems employing Dre recombinase |
| EP1942192A1 (en) | 2007-01-08 | 2008-07-09 | Heinrich-Pette-Institut für experimentelle Virologie und Immunologie | Use of a tailored recombinase for the treatment of retroviral infections |
| DE102007001370A1 (de) | 2007-01-09 | 2008-07-10 | Curevac Gmbh | RNA-kodierte Antikörper |
| WO2009007982A1 (en) | 2007-07-11 | 2009-01-15 | State Of Israel, Ministry Of Agriculture, Agricultural Research Organization | A conserved region of the hiv-1 genome and uses thereof |
| WO2009146179A1 (en) | 2008-04-15 | 2009-12-03 | University Of Iowa Research Foundation | Zinc finger nuclease for the cftr gene and methods of use thereof |
| US8645115B2 (en) | 2008-12-22 | 2014-02-04 | Trustees Of Boston University | Modular nucleic acid-based circuits for counters, binary operations, memory and logic |
| DE102008063216A1 (de) | 2008-12-29 | 2010-07-01 | Wilhelm Karmann Gmbh | Verdeck eines Cabriolet-Fahrzeugs mit wenigstens zwei Dachsegmenten und einem Innenhimmel |
| JP5336592B2 (ja) | 2009-06-08 | 2013-11-06 | 公益財団法人かずさＤｎａ研究所 | 新規な部位特異的組換え酵素とその認識配列を用いた部位特異的組換え方法 |
| ES2577929T3 (es) | 2010-05-27 | 2016-07-19 | Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - | Recombinasa elaborada a medida para recombinar unos sitios diana asimétricos en una pluralidad de cepas de retrovirus |
| FI4005592T3 (fi) | 2010-07-06 | 2023-01-13 | Virionin kaltaisia kuljetuspartikkeleita itsereplikoituville rna-molekyyleille | |
| WO2012088381A2 (en) | 2010-12-22 | 2012-06-28 | President And Fellows Of Harvard College | Continuous directed evolution |
| EP2690177B1 (en) | 2012-07-24 | 2014-12-03 | Technische Universität Dresden | Protein with recombinase activity for site-specific DNA-recombination |
| WO2014093330A1 (en) | 2012-12-10 | 2014-06-19 | Clearfork Bioscience, Inc. | Methods for targeted genomic analysis |
| US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
| ES2750550T3 (es) | 2013-03-01 | 2020-03-26 | Univ Minnesota | Corrección de gen a base de TALEN |
| GB201414130D0 (en) | 2014-08-08 | 2014-09-24 | Agency Science Tech & Res | Mutants of the bacteriophage lambda integrase |
| EP2993229A1 (en) * | 2014-09-02 | 2016-03-09 | Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - | Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains |
| US20170266320A1 (en) * | 2014-12-01 | 2017-09-21 | President And Fellows Of Harvard College | RNA-Guided Systems for In Vivo Gene Editing |
| CN106191041B (zh) | 2015-04-30 | 2020-12-29 | 杭州菁因康生物科技有限公司 | 基因打靶方法 |
| US10392674B2 (en) | 2015-07-22 | 2019-08-27 | President And Fellows Of Harvard College | Evolution of site-specific recombinases |
| US10017832B2 (en) | 2015-08-25 | 2018-07-10 | Washington University | Compositions and methods for site specific recombination at asymmetric sites |
| US11078493B2 (en) | 2016-06-21 | 2021-08-03 | Nanyang Technological University | Site-specific DNA recombination |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018229226A1 (en) | 2017-06-14 | 2018-12-20 | Technische Universität Dresden | Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes |
| US20220267802A1 (en) | 2019-07-15 | 2022-08-25 | President And Fellows Of Harvard College | Methods and compositions for gene delivery |
| EP3831939A1 (en) | 2019-12-06 | 2021-06-09 | Technische Universität Dresden | Fusion of site-specific recombinases for efficient and specific genome editing |
| WO2021158651A1 (en) | 2020-02-04 | 2021-08-12 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods for controlling production of polypeptides in cells |
| WO2021207401A1 (en) | 2020-04-07 | 2021-10-14 | Io Biosciences, Inc. | Nucleic acid constructs comprising gene editing multi-sites |
| GB202005180D0 (en) | 2020-04-08 | 2020-05-20 | Ge Healthcare Bio Sciences Ab | Methods for targeted integration |
-
2018
- 2018-06-14 WO PCT/EP2018/065881 patent/WO2018229226A1/en not_active Ceased
- 2018-06-14 FI FIEP18731446.3T patent/FI3638776T3/fi active
- 2018-06-14 US US16/622,937 patent/US11845954B2/en active Active
- 2018-06-14 JP JP2019568653A patent/JP7402503B2/ja active Active
- 2018-06-14 DK DK18731446.3T patent/DK3638776T3/da active
- 2018-06-14 CN CN201880039699.9A patent/CN110914414A/zh active Pending
- 2018-06-14 EP EP18731446.3A patent/EP3638776B1/en active Active
- 2018-06-14 CA CA3066047A patent/CA3066047A1/en active Pending
-
2022
- 2022-10-12 US US18/046,066 patent/US20230235364A1/en active Pending
-
2023
- 2023-06-05 JP JP2023092153A patent/JP2023116580A/ja active Pending
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