JP7402503B2 - デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 - Google Patents

デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 Download PDF

Info

Publication number
JP7402503B2
JP7402503B2 JP2019568653A JP2019568653A JP7402503B2 JP 7402503 B2 JP7402503 B2 JP 7402503B2 JP 2019568653 A JP2019568653 A JP 2019568653A JP 2019568653 A JP2019568653 A JP 2019568653A JP 7402503 B2 JP7402503 B2 JP 7402503B2
Authority
JP
Japan
Prior art keywords
sequence
dna
sequences
target
recombinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019568653A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020527938A5 (enExample
JP2020527938A (ja
Inventor
ブチホルツ フランク
シュナイダー マーティン
ランシング フェリックス
Original Assignee
テクニスチェ ユニベルシタト ドレスデン
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by テクニスチェ ユニベルシタト ドレスデン filed Critical テクニスチェ ユニベルシタト ドレスデン
Publication of JP2020527938A publication Critical patent/JP2020527938A/ja
Publication of JP2020527938A5 publication Critical patent/JP2020527938A5/ja
Priority to JP2023092153A priority Critical patent/JP2023116580A/ja
Application granted granted Critical
Publication of JP7402503B2 publication Critical patent/JP7402503B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1058Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Ecology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2019568653A 2017-06-14 2018-06-14 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段 Active JP7402503B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023092153A JP2023116580A (ja) 2017-06-14 2023-06-05 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP17175895 2017-06-14
EP17175895.6 2017-06-14
PCT/EP2018/065881 WO2018229226A1 (en) 2017-06-14 2018-06-14 Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2023092153A Division JP2023116580A (ja) 2017-06-14 2023-06-05 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段

Publications (3)

Publication Number Publication Date
JP2020527938A JP2020527938A (ja) 2020-09-17
JP2020527938A5 JP2020527938A5 (enExample) 2021-07-26
JP7402503B2 true JP7402503B2 (ja) 2023-12-21

Family

ID=59269741

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2019568653A Active JP7402503B2 (ja) 2017-06-14 2018-06-14 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段
JP2023092153A Pending JP2023116580A (ja) 2017-06-14 2023-06-05 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2023092153A Pending JP2023116580A (ja) 2017-06-14 2023-06-05 デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段

Country Status (8)

Country Link
US (2) US11845954B2 (enExample)
EP (1) EP3638776B1 (enExample)
JP (2) JP7402503B2 (enExample)
CN (1) CN110914414A (enExample)
CA (1) CA3066047A1 (enExample)
DK (1) DK3638776T3 (enExample)
FI (1) FI3638776T3 (enExample)
WO (1) WO2018229226A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3066047A1 (en) 2017-06-14 2018-12-20 Technische Universitat Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
TW202124722A (zh) 2019-09-18 2021-07-01 美商英特佳樂帝克醫療公司 合成dna載體及其使用方法
EP3831939A1 (en) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
AU2022386716A1 (en) 2021-11-15 2024-05-02 Technische Universität Dresden Site-specific recombinases for efficient and specific genome editing
WO2025143215A1 (ja) * 2023-12-28 2025-07-03 デンカ株式会社 多型塩基を組み込んだ標的配列を作製するための組換えベクター
WO2025252950A1 (en) 2024-06-07 2025-12-11 Technische Universität Dresden Körperschaft Des Öffentlichen Rechts Method for producing spacer-specific dna recombining enzymes

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170058297A1 (en) 2015-08-25 2017-03-02 Washington University Compositions and methods for site specific recombination at asymmetric sites

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4861719A (en) 1986-04-25 1989-08-29 Fred Hutchinson Cancer Research Center DNA constructs for retrovirus packaging cell lines
US5278056A (en) 1988-02-05 1994-01-11 The Trustees Of Columbia University In The City Of New York Retroviral packaging cell lines and process of using same
US5670488A (en) 1992-12-03 1997-09-23 Genzyme Corporation Adenovirus vector for gene therapy
DE69434860T2 (de) 1993-02-22 2007-03-15 The Rockefeller University Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion
FR2712812B1 (fr) 1993-11-23 1996-02-09 Centre Nat Rech Scient Composition pour la production de produits thérapeutiques in vivo.
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
US6774279B2 (en) 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
US5981830A (en) 1997-05-30 1999-11-09 Schering Aktiengesellschaft Knockout mice and their progeny with a disrupted hepsin gene
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
CA2306184C (en) 1997-11-18 2007-05-15 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
NZ504511A (en) 1997-11-18 2002-12-20 Pioneer Hi Bred Int Recombinant proteins comprising fused first and second distinct site specific recombinase and use in methods for the integration of foreign DNA into eukaryotic genomes
DE19941186A1 (de) 1999-08-30 2001-03-01 Peter Droege Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
GB0029375D0 (en) 2000-12-01 2001-01-17 Total Fina Elf Substrate linked directed evolution (slide)
DE10140030C1 (de) 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System
US20090217400A1 (en) 2004-02-26 2009-08-27 State Of Israel, Ministry Of Agriculture, Agricultural Research Organization Enzymes, cells and methods for site specific recombination at asymmetric sites
US7422889B2 (en) 2004-10-29 2008-09-09 Stowers Institute For Medical Research Dre recombinase and recombinase systems employing Dre recombinase
EP1942192A1 (en) 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Use of a tailored recombinase for the treatment of retroviral infections
DE102007001370A1 (de) 2007-01-09 2008-07-10 Curevac Gmbh RNA-kodierte Antikörper
WO2009007982A1 (en) 2007-07-11 2009-01-15 State Of Israel, Ministry Of Agriculture, Agricultural Research Organization A conserved region of the hiv-1 genome and uses thereof
WO2009146179A1 (en) 2008-04-15 2009-12-03 University Of Iowa Research Foundation Zinc finger nuclease for the cftr gene and methods of use thereof
US8645115B2 (en) 2008-12-22 2014-02-04 Trustees Of Boston University Modular nucleic acid-based circuits for counters, binary operations, memory and logic
DE102008063216A1 (de) 2008-12-29 2010-07-01 Wilhelm Karmann Gmbh Verdeck eines Cabriolet-Fahrzeugs mit wenigstens zwei Dachsegmenten und einem Innenhimmel
WO2010143606A1 (ja) 2009-06-08 2010-12-16 財団法人かずさディー・エヌ・エー研究所 新規な部位特異的組換え酵素とその認識配列を用いた部位特異的組換え方法
RU2617968C2 (ru) 2010-05-27 2017-04-28 Хайнрих-Петте-Институт, Ляйбниц-Институт Фюр Экспериментелле Фирологи-Штифтунг Бюргерлихен Рехтс Адаптированная рекомбиназа для рекомбинации асимметричных участков-мишеней во множестве штаммов ретровирусов
HRP20161352T1 (hr) 2010-07-06 2016-12-02 Glaxosmithkline Biologicals Sa Čestice nalik na virione za unos samoreplicirajućih molekula rna
CA2825370A1 (en) 2010-12-22 2012-06-28 President And Fellows Of Harvard College Continuous directed evolution
EP2690177B1 (en) 2012-07-24 2014-12-03 Technische Universität Dresden Protein with recombinase activity for site-specific DNA-recombination
WO2014093330A1 (en) 2012-12-10 2014-06-19 Clearfork Bioscience, Inc. Methods for targeted genomic analysis
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US9393257B2 (en) 2013-03-01 2016-07-19 Regents Of The University Of Minnesota TALEN-based gene correction
GB201414130D0 (en) 2014-08-08 2014-09-24 Agency Science Tech & Res Mutants of the bacteriophage lambda integrase
EP2993229A1 (en) * 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Well-tolerated and highly specific tailored recombinase for recombining asymmetric target sites in a plurality of retrovirus strains
US20170266320A1 (en) 2014-12-01 2017-09-21 President And Fellows Of Harvard College RNA-Guided Systems for In Vivo Gene Editing
CN106191041B (zh) 2015-04-30 2020-12-29 杭州菁因康生物科技有限公司 基因打靶方法
WO2017015545A1 (en) 2015-07-22 2017-01-26 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
CA3033327A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
CA3066047A1 (en) 2017-06-14 2018-12-20 Technische Universitat Dresden Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes
WO2021015997A1 (en) 2019-07-15 2021-01-28 President And Fellows Of Harvard College Methods and compositions for gene delivery
EP3831939A1 (en) 2019-12-06 2021-06-09 Technische Universität Dresden Fusion of site-specific recombinases for efficient and specific genome editing
US20230064644A1 (en) 2020-02-04 2023-03-02 The Board Of Trustees Of The Leland Stanford Junior University Compositions and Methods for Controlling Production of Polypeptides in Cells
EP4133086A4 (en) 2020-04-07 2024-06-05 IO Biosciences, Inc. NUCLEIC ACID CONSTRUCTS WITH GENE-EDITING MULTI-SITES
GB202005180D0 (en) 2020-04-08 2020-05-20 Ge Healthcare Bio Sciences Ab Methods for targeted integration

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170058297A1 (en) 2015-08-25 2017-03-02 Washington University Compositions and methods for site specific recombination at asymmetric sites

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J. Mol. Biol.,2008年05月02日,Vol. 378, No. 3,pp. 653-665
Nature Biotechnology,2016年04月,Vol. 34, No. 4,pp. 401-409
Nucleic Acids Research,2006年09月20日,Vol. 34, No. 18,pp. 5259-5269

Also Published As

Publication number Publication date
US20210147878A1 (en) 2021-05-20
FI3638776T3 (fi) 2025-12-09
EP3638776B1 (en) 2025-11-05
CN110914414A (zh) 2020-03-24
JP2023116580A (ja) 2023-08-22
CA3066047A1 (en) 2018-12-20
DK3638776T3 (da) 2025-12-15
JP2020527938A (ja) 2020-09-17
US20230235364A1 (en) 2023-07-27
WO2018229226A1 (en) 2018-12-20
US11845954B2 (en) 2023-12-19
EP3638776A1 (en) 2020-04-22

Similar Documents

Publication Publication Date Title
JP7402503B2 (ja) デザイナーdna組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段
US20240352489A1 (en) Methods and compositions for modifying a targeted locus
JP7383062B2 (ja) 配列操作のための最適化されたCRISPR-Cas二重ニッカーゼ系、方法および組成物
AU2021201624B2 (en) Large gene excision and insertion
JP6896786B2 (ja) 配列操作のためのCRISPR−Cas成分系、方法および組成物
Sugano et al. Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha
US10314297B2 (en) DNA knock-in system
JP2024023194A (ja) 肝臓ターゲティングおよび治療のためのCRISPR-Cas系、ベクターおよび組成物の送達および使用
US11530421B2 (en) Self-inactivating endonuclease-encoding nucleic acids and methods of using the same
US20200224221A1 (en) Genome editing method
Porteus Using homologous recombination to manipulate the genome of human somatic cells
Zhang et al. Rapid assembly of customized TALENs into multiple delivery systems
WO2019028686A1 (zh) 基因敲除方法
Sato Engineered Recombinases: Tools for Therapeutic Human Genome Editing
WO2025091593A1 (zh) 一种基于sgRNA-供体DNA嵌合体的基因编辑系统
HK40035693A (en) Methods and compositions for modifying a targeted locus
Hryhorowicz et al. Programmable site-specific nucleases for target genome engineering
HK1235817A1 (en) Methods and compositions for modifying a targeted locus
HK1235817B (en) Methods and compositions for modifying a targeted locus

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200706

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210611

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210611

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20220408

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220419

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220704

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221019

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20221019

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20230207

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230605

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20230605

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20230712

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230926

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20231004

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20231121

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20231204

R150 Certificate of patent or registration of utility model

Ref document number: 7402503

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150