US20230235364A1 - Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes - Google Patents

Methods and means for genetic alteration of genomes utilizing designer dna recombining enzymes Download PDF

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US20230235364A1
US20230235364A1 US18/046,066 US202218046066A US2023235364A1 US 20230235364 A1 US20230235364 A1 US 20230235364A1 US 202218046066 A US202218046066 A US 202218046066A US 2023235364 A1 US2023235364 A1 US 2023235364A1
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Frank Buchholz
Martin Schneider
Felix LANSING
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Technische Universitaet Dresden
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Definitions

  • the invention provides methods and means for specifically altering the DNA sequence in a genome.
  • the invention is useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. Further, the invention is useful for biological and biomedical research (e. g. to create animal models).
  • DNA recombining enzymes in particular site-specific recombinase (SSR) systems, allow precise manipulation of DNA without triggering endogenous DNA repair pathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processed DNA in vivo.
  • SSR systems such as Cre/loxP (EP 0 2200 009 B1) have found widespread use in model organisms, demonstrating that they can be safe, even when expressed lifelong in animals. SSRs work as tetramers on their target sites and depending on the orientation of these sites, recombination can lead to a variety of outcomes. It is known that the amino acid sequence of Cre can be modified in order to obtain novel site-specific recombinases (Buchholz F and Stewart AF 2001).
  • SSR systems know in the art include the Flp/FRT system (WO 1999025841 A1 and U.S. Pat. No. 6,774,279 B2), the Dre/rox system (U.S. Pat. No. 7,422,889 B2 and U.S. Pat. No. 7,915,037 B2), the VCre/VloxP system and the sCre/SloxP system (WO 2010/143606 A1), as well as the Vika/vox, the Nigri/nox and the Panto/pox systems (WO 2014/016248 A1).
  • Naturally occurring DNA recombining enzymes in particular site-specific recombinase (SSR) systems (such tyrosine-type SSRs), generally consist of four identical monomers. In general they recognize two identical and symmetric, palindromic target sites, which consist each of two 13 nucleotide long half sites separated by an asymmetric frequently 8 nucleotide long spacer. Depending on the number and relative orientation of the target sites the DNA recombining enzyme either causes a deletion, an insertion, an inversion or a replacement of genetic content.
  • SSR site-specific recombinase
  • a bottleneck for using DNA recombining enzymes in therapeutic genome editing has been the limited number of sequences they can recombine.
  • WO2009007982A1 describes nucleic acid sequences located in the LTR of HIV-1 as potential target sites for DNA recombining enzymes.
  • US2009/0217400 A1 describes an enzyme and a method for recombination at asymmetric loxP-M7 target sites. Further, Zhang, C et al. (2015) and US 20170058297 A1 describe an obligate heterotetramer complex of two Cre mutants that recognize asymmetric loxP-M7 target sites. However, these recognition sites have to be introduced artificially into the genome.
  • US 20040003435 A1 describes a method for targeted insertion of a sequence of interest into a plant said method comprising introducing a transfer cassette comprising said nucleotide sequence of interest flanked by or comprising non-identical recognition sites, which are modified FRT sites that can still be recognized by the wild-type FLP recombinase.
  • RNA-guided nucleases like CRISPR/Cas9 (e. g. U.S. Pat. No. 8,657,359 B1) as the specificity of these enzymes for a target site is defined by the guide RNA, which can be versatility adapted to a sequence of interest.
  • nucleases systems need the cell intrinsic DNA repair mechanisms they typically induce an abundance of random insertions and deletions (indels) at the target locus.
  • the ideal genome editing or gene therapy will replace the defective gene with a normal allele or delete a defective gene at its natural location without further modification of the genome.
  • the ideal genome editing should be efficient and specific, without the introduction of indels.
  • An objective of the invention is therefore to provide a method to alter a nucleotide sequence in a genome that overcomes the disadvantages of the nuclease genome editing approaches and which is at the same time versatile adaptable to a sequence of interest.
  • the invention is based on the finding that two identical sequences with a length of 34 base pairs (the length of a usual target site of a DNA recombining enzyme) are rarely found in a genome.
  • the invention starts with the realization of the inventors that the only restriction to achieve excision recombination for Designer DNA-recombining enzymes is the conservation of the spacer sequence, normally 8 bp long (see FIG. 17 for an example). Because this sequence has to be identical for recombination to occur, it sets a constraint for sequences that can be recombined. However, a bioinformatics analysis of different genomes revealed that direct repeats of 8 bp length are frequently occurring in viral, prokaryotic and eukaryotic genomes, including the human genome (see FIG. 18 , 19 for an example).).
  • the invention overcomes the limitation of known DNA-recombining enzymes in respect of the target sites they can recombine and provides designer DNA recombining enzymes that are useful for genome editing that can recognize target sequences occurring naturally in a genome.
  • the invention provides designer DNA-recombining enzymes that are capable to induce a site-specific DNA recombination to alter a nucleotide sequence in a genome by recombining two non-identical target sequences (preferably naturally) occurring in the genome and a method for preparing the designer DNA-recombining enzymes.
  • the designer DNA-recombining enzymes according to the invention advantageously does not need target sites that are artificially introduced in the genome and further does not require identical sequences with a length over 8 nucleotides (in particular not 34 nucleotides) to be present, like long terminal repeats (LTRs).
  • LTRs long terminal repeats
  • Non-identical target sequences mean in the context of this invention that the target sites differ in at least two nucleotides.
  • the target sites may differ even more, in particular in at least three nucleotides.
  • the target sites differ in at least four nucleotides or even eight nucleotides.
  • at least 20% or even more preferred 30% of the nucleotides and up to 70% or even up to 90% of the nucleotides in the target sites can differ, which means that the target site may have a sequence identity of only 10 to 80%, preferably 30 to 90%.
  • Each target site comprises a first half site (preferably 10 to 20 nucleotides) and a second half site (preferably 10 to 20 nucleotides) separated by a spacer sequence (preferably 5 to 12 nucleotides).
  • a first half site preferably 10 to 20 nucleotides
  • a second half site preferably 10 to 20 nucleotides
  • a spacer sequence preferably 5 to 12 nucleotides.
  • one or even both target sites may be asymmetric, meaning that the half sites of the target site are not reverse-complementary to each other.
  • at least 20% or even more preferred 30% of the nucleotides and up to 70% or even up to 90% of the nucleotides in the half sites can differ, which means that all the half sites may have a sequence identity of only 10 to 80%, preferably 30 to 90%.
  • the differences in the target sites lie only in the half sites and the spacer sequences are identical to induce deletion of the sequence of interest.
  • the two spacer sequences are also different to enable replacement of the
  • the designer DNA-recombining enzymes according to the invention comprises one or two or up to four different monomers.
  • the designer DNA-recombining enzyme comprises four different monomers and each monomer recognizes a different half site:
  • the designer DNA-recombining enzyme comprises two or three different monomers whereas one or two monomers recognize an asymmetric target site.
  • the designer DNA-recombining enzyme comprises four identical monomers whereas the designer DNA-recombining enzyme recognize two different asymmetric target sites.
  • Different monomers according to the invention means that the monomers differ in at least 2%, in particular at least 4%, preferably at least 8% of the amino acid residues.
  • Advantageously up to 10% or even up to 30% of the amino acid residues may differ, which means that the monomers may have a sequence identity of only 70 to 98%, preferably 90 to 96% or up to 92%.
  • DNA-recombining enzyme according to the invention is either a homotetramer or a heterotetramer comprising two or up to four different monomers.
  • the monomer-monomer interfaces are genetically modified in such a way that only distinct monomers can form tetramers (e. g. as described in the publication Zhang et al. 2015 and in and US 20170058297 A1). By this modification an obligate heterotetramer is formed excluding tetramers comprising only one monomer.
  • the sequence of interest is a nucleotide sequence to be altered. It is preferably a sequence comprising mutation.
  • mutation includes all kind of genetic or chromosomal aberrations, in particular point mutations, frame shift mutations, translocations, duplications, deletions or insertions.
  • the sequence of interest is a sequence that shall be inactivated, in particular a sequence of a pathogen (a microorganism like a bacteria, a virus or a parasite) or an oncogene or another gene, whereas the activity causes a disease or the inhibition of gene activity is known to reduce symptoms.
  • a pathogen a microorganism like a bacteria, a virus or a parasite
  • an oncogene or another gene whereas the activity causes a disease or the inhibition of gene activity is known to reduce symptoms.
  • PCSK9 Protein convertase subtilisin/kexin type 9
  • the recombination induced by the DNA-recombining enzyme according to the invention preferably results in a deletion of the inserted sequence or the sequence that shall be inactivated.
  • This deletion further also referred to as Designer DNA Recombining enzyme induced Gene Deletion (DRiGD)—is achieved by two identical spacer sequences in the two target sites (s. also FIG. 2 for an illustrative example).
  • the recombination induced by the DNA-recombining enzyme according to the invention results in a replacement of the sequence of interest by a desired sequence.
  • This replacement further also referred to as Designer DNA Recombining enzyme induced Gene Replacement (DRiGR)—is achieved by providing a synthetic donor sequence comprising the first target site and the second target site, whereas the first and second target site surround the desired sequence.
  • DRiGR Designer DNA Recombining enzyme induced Gene Replacement
  • the DNA recombining enzymes according to the invention allow precise genome editing without triggering endogenous DNA repair pathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processed DNA in vivo.
  • DNA recombining enzyme comprises every enzyme that is capable to induce a site-specific DNA recombination event, preferably a recombinase or integrase, in particular selected from enzymes that carry out a topoisomerase-like reaction, such as serine or tyrosine recombinase families.
  • the known DNA-recombining enzymes whose target sequences are used in step a) and upon which molecular directed evolution is applied in steps (b) are selected from Cre, Dre, VCre, sCre, FLP, Tre (including Tre 1.0 to Tre 3.1 and Bred), Vika, Nigri and Panto.
  • designer DNA recombining enzyme refers to the facts that by the invention DNA recombining enzymes are generated to a given target site and that the DNA recombining enzymes do not occur in nature.
  • the invention relates to a method for identifying sequences that are potential target sites for DNA-recombining enzymes that are capable to induce a site-specific DNA recombination of a sequence of interest in a genome, including the steps of:
  • This method is in particular suitable to screen for identifying sequences that are potential target sites for DNA-recombining enzymes that induce a deletion (DRiGD) of a sequence of interest in a genome.
  • the genome is screened in step i. for two identical sequences that are potential spacer sequences. This method is also illustrated by the example in FIG. 18 .
  • the sequence of interest to be deleted is a sequence in the genome of the host, e. g. an oncogene or another gene that shall be inactivated.
  • the genome screened in step i. is the genome of the host itself (or a part thereof comprising the sequence of interest), e. g. the human genome or part of a human chromosome.
  • the potential target sequences are preferably screened not to occur elsewhere (thus only around the sequence of interest) in the genome of the host to insure a sequence specific deletion.
  • the sequence of interest to be deleted is a sequence in the genome of a pathogen, e. g. a virus.
  • step i. is performed on the genome of the pathogen and step iii. on the genome of the host, which is infected by the pathogen.
  • the potential target sequences are preferably screened to occur not in the genome of the host (thus only in the genome of the pathogen) to insure a sequence specific deletion.
  • target sequences are selected from the list of potential target sequences obtained in step ii or iii. that comprise half sites that have each a homology to a half site of a known target site of a DNA-recombining enzyme.
  • Homology means that a certain number of nucleotide positions bears identical bases.
  • each half site has at least 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to a half site of a known target sites of a DNA-recombining enzyme.
  • the half sites preferably a have a homology between each other.
  • each half site has at least
  • target half-sites 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to the other half site of the same target sites and/or the half sites of the other target site.
  • the homology between target half-sites increases the chance that a monomer can be generated that is active on both half-sites and therefore the number of monomers necessary for recombination is reduced.
  • step iii. to insure a sequence specific deletion can be performed either before or after step iv.
  • the coordinates of the sequence of interest to be inactivated is determined in the genome.
  • Search windows of 150 bp to 10 kb kB fragments covering the sequence of interest and surrounding sequences are generated. Fragments overlapping with other genes are removed. For all other fragments, potential identical spacer sequences—thus spacer sequences that are repeated at least once are identified.
  • spacer sequences are selected before step ii. that surround the sequence of interest (e. g. an exon of the gene to be inactivated or at least overlap with the sequence of interest (e. g. the exon).
  • potential target sites are identified as described in step ii and subsequently pairs of target sites are selected that surround the sequence of interest (e. g. an exon of the gene to be inactivated) or at least overlap with the sequence of interest (e. g. the exon).
  • the method is used for identifying sequences that are potential target sites for DNA-recombining enzymes that induce a replacement (DRiGR) of a sequence of interest in a genome.
  • the genome is screened in step i. for two sequences that are potential spacer sequences and are not identical.
  • the potential spacer sequences differ in at least 30% to 100% preferably at least 50% of the nucleotides
  • the potential spacer sequences preferably have a sequence identity of 0% to 50 or at least below 70%.
  • sequences that are potential target sites for DNA-recombining enzymes that induce a replacement (DRiGR) of a sequence of interest in a genome are identified by a method including the steps of:
  • target sequences are selected from the list of potential target sequences obtained in step i or ii. that comprise half sites that have each a homology to a half site of a known target site of a DNA-recombining enzyme.
  • Homology means that a certain number of nucleotide positions bears identical bases.
  • each half site has at least 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to a half site of a known target sites of a DNA-recombining enzyme.
  • step ii. to insure a sequence specific replacement can be performed either before or after step iii.
  • the coordinates of the sequence of the sequence of interest (preferably a gene or exon) to be replaced is determined in the genome.
  • Search windows of 150 bp to 10 kb kB fragments covering the sequence of interest and surrounding sequences are generated. Fragments overlapping with other genes are removed. For all other fragments, potential target sites are identified.
  • spacer sequences are selected that surround the sequence of interest (e. g. an exon of the gene to be replaced) or at least overlap with the sequence of interest (e. g. the exon).
  • the method for preparing the designer DNA-recombining enzymes according to the invention enzymes that are capable to induce a site-specific DNA recombination to alter a nucleotide sequence in a genome by recombining two target sequences naturally occurring in the genome comprises the following steps:
  • the designer DNA-recombining enzyme may be selected to target any pair of target sequences and consequently any sequence of interested flanked by these target sites.
  • the target sequences are naturally occurring sequences within a genome of a host (e. g. a cell or organism) or in the genome of a pathogen, like a virus. “Naturally occurring sequences within a genome” means in the context of the invention that the target sequences are not artificially introduced and that both non-identical sequences occur natively (naturally) in the same genome.
  • Genome is defined as all the genetic material of an organism or pathogen. It generally consists of DNA (or RNA in RNA viruses). The genome includes the genes (the coding regions ore exons), the noncoding DNA (like introns and regulatory sequences) and the genetic material of the mitochondria and chloroplasts.
  • Preferred target sequences include those that are unique in the target genome of a host cell or organism. As some organism have multiple copies of chromosomes, unique means in this context that they occur only once per set of chromosome.
  • the inventors have surprisingly found (see example F9-3, which can recombine both loxF9a and loxF9b) that one DNA-recombining enzyme can be used to recombine two different target sites in case all the half sites differ between each other in 7 to 9 of 13 positions.
  • one library of DNA-recombining enzymes can be used to evolve an enzyme that can recombine both target sites, in particular in a DRiGR reaction (see FIGS. 15 and 16 and Table on page 63 for an example).
  • the half sites show a pairwise difference of a maximum of 7, preferably 5, more preferably 4 mismatches—thus both target sites show a maximum of 7, preferably 5 or more preferably 4 mismatches between the half sites.
  • 2 libraries of DNA-recombining enzymes are used to evolve an enzyme that can recombine both target sites.
  • one target site shows a pair of half sites with a maximum of 7, preferably 5, more preferably 4 mismatches—thus the other target site differs in more than 7, preferably 5, more preferably 4 mismatches and also the half sites of the other target site show more than 7, preferably 5, more preferably 4 mismatches compared to each other.
  • 3 or 4 libraries of DNA-recombining enzymes are used to evolve an enzyme that can recombine both target sites.
  • the library or libraries of DNA-recombining enzymes used in step b) are evolved to obtain one monomer of DNA-recombining enzymes or co-evolved to obtain at least two monomers of DNA-recombining enzymes that work together on the pair of target sites.
  • two or more libraries of DNA-recombining enzymes used in step b) they are preferably operationally linked, preferably on one expression vector.
  • the libraries are preferably expressed under the control of the same regulatory elements, preferably the same promoter.
  • one or two to four libraries of DNA-recombining enzymes are used in step b) for coevolution.
  • two or four libraries of DNA-recombining enzymes are used in step b).
  • the DNA-recombining enzymes in each library advantageously recognize different asymmetric target sites—target sites composed of two different half sites.
  • the DNA-recombining enzymes in each library preferably recognizes each a different half site of an asymmetric target site. Thus, they only recombine the target site, when co-expressed as heterotetramer.
  • the coevolution in step b) is performed using an expression vector that encodes for the library of DNA-recombining enzymes or the at least two different libraries of DNA-recombining enzymes and comprising the first and second target site.
  • the expression vector encodes for two or four libraries of DNA-recombining enzymes.
  • a negative selection marker lies in between the first and second target site.
  • the first and second target site preferably surround a negative selection marker.
  • the negative selection marker is in a preferred embodiment a recognition site for a restriction enzyme.
  • a unique recognition site meaning a recognition site for a restriction enzyme that occurs only once in the vector.
  • the negative selection comprises more than one recognition site for a restriction enzyme, preferably two or three (or even up to five) recognition sites for the same or in particular for two to three (or even up to five) different restriction enzymes.
  • an exonuclease is preferably added to digest the linearized DNA.
  • the substrate nucleic acid comprising the first target site and the second target site is preferably situated on the same vector as the DNA encoding the library of DNA-recombining enzymes or the at least two different libraries of DNA-recombining enzymes (as exemplified in FIGS. 4 and 7 ).
  • the method comprises also the transduction of a cell with the expression vector as defined above and expression of the DNA-recombining enzyme.
  • a cell-free expression system is used.
  • the DNA-recombining enzyme according to the invention shall result in a deletion (DRiGD), preferably no further DNA substrates or vectors are used for one recombination reaction.
  • a successful recombination cleaves out the negative selection marker and the plasmid is amplified—preferably by PCR (as exemplified in FIGS. 6 and 7 —pDuoSLiDE-DRiGD, pQuSLiDE-DRiGD).
  • the first and second target sequences comprises identical spacer sequences (s. also FIG. 2 ).
  • the method for identifying sequences that are potential target sites for DNA-recombining enzymes described above is preferably performed when or before selecting the target sites in step a) of the method for preparing designer DNA-recombining enzymes for deletion or replacement.
  • the DNA-recombining enzyme according to the invention shall result in a replacement (DRiGR) a synthetic sequence comprising the first target site and the second target site is used in addition to the substrate.
  • the first and second target sequences comprise different spacer sequences to avoid a deletion to occur. Consequently, the recombination is shifted versus replacement by recombining the identical target sequences on the substrate (on the vector encoding the libraries) and the synthetic sequence.
  • the negative selection marker is replaced by recombining with the synthetic sequence.
  • the synthetic sequence is a replication deficient plasmid (s. also FIGS. 4 and 5 for examples).
  • a linear DNA strand is used.
  • the synthetic sequences are on a high-copy number plasmid (s. FIG. 14 for an example).
  • the synthetic sequence preferably comprises a positive selection marker between the first and second target.
  • the sequence with the positive selection marker will be inserted into the expression vector and expression vectors bearing the positive selection marker can be positively selected.
  • the positive selection marker is an antibiotic-resistance gene.
  • the vector bears a negative selection marker between the first and second target site and the synthetic sequence additionally comprises a positive selection marker between the first and second target site.
  • negative and positive selection markers are known to a person skilled in the art.
  • Further applicable negative selection markers include gene encoding enzymes that encode a gene encoding a toxin or genes that convert a prodrug into a toxic drug, like URA3 (converting 5-fluoroorotic acid into the toxic compound 5-fluorouracil) or viral thymidine kinases (making the host sensitive to ganciclovir selection).
  • Further applicable positive selection markers are genes encoding for enzymes that can complement an auxotrophy.
  • One step of molecular evolution preferably comprises at least one selection step and at least one step of mutagenesis.
  • the selected candidates are preferably mutagenized so as to introduce mutations into the sequence for testing in the next round of selection.
  • Suitable methods of mutagenesis are known to those of skill in the art and include in particular error-prone PCR, chemical mutagenesis, the use of specific mutator host strains, recursive ensemble mutagenesis, combinatorial cassette mutagenesis and DNA shuffling as well as a combination of these methods.
  • One or more, preferably, five to fifty, preferably eight to thirty, cycles of co-evolution are preferably be carried out, until DNA-recombining enzymes are evolved that are active on the target sites.
  • the co-evolution step b) can be directly performed on existing libraries of DNA-recombining enzymes.
  • the method of the invention comprises the following further steps to obtain the two libraries of DNA-recombining enzymes used in step b).
  • step a) is further referred to as step a1)
  • Selecting a1) and Identifying a2) are preferably performed simultaneously in one step a), e. g. by a computer program, like the SeLOX algorithm (Surendranath, V. et al. (2010).
  • a computer program like the SeLOX algorithm (Surendranath, V. et al. (2010).
  • the sequences up- and down-stream of the site of interest are compared with known target sites of DNA-recombining enzymes and a target sequence wherein each half site has a certain homology to a half site of a known target site of a DNA-recombining enzyme is selected as described in a1).
  • the half sites of the known target sites identified in this way are the ones mentioned in a2).
  • the degree of homology is preferably as mentioned in a1).
  • step a1 preferably intermediate target sites are generated that are a mix between the half sites identified in step a2) and the target sites as selected in step a1).
  • the intermediate target sites are preferably generated by determining the nucleotides within the first to fourth sequences (as defined in a2)) that differ from the target sites selected in a1) and replacing one or more, preferably up to three nucleotides that differ by the nucleotides that occur in the corresponding half sites of the target site.
  • the intermediate target sites are symmetric, thus they comprise identical or reverse complementary half sites.
  • a set of four intermediate target sites is generated—one intermediate target site for each half site of the target sites selected in a1).
  • the first intermediate first target site comprises one sequence that corresponds to the first sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the first half site of the first target site (as selected in step a1)) and a sequence that corresponds to the second sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the second half site of the first target site (as selected in a1)).
  • the second intermediate target site that comprises a sequence that corresponds to the third sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the first half site of the second target site (as selected in step a1)) and a sequence that corresponds to the fourth sequence (as de-fined in a2)) in which nucleotides are replaced by nucleotides occurring in the second half site of the second target site (as selected in step a1)).
  • step a2 Applying molecular directed evolution on DNA-recombining enzymes recognizing the sequences defined in step a2) using a nucleic acid comprising the intermediate first target site and/or the intermediate second target site as substrate.
  • the nucleic acid comprises both the intermediate first target site and the intermediate second target and a direct evolution of DNA-recombining enzymes is performed on both intermediate target sites (direct coevolution).
  • the coevolution is preferably performed as described above for step b).
  • the first rounds of molecular directed evolution are performed as a separated molecular directed evolution of DNA-recombining enzymes (separate evolution) for each intermediate target site (e g. as depicted in FIG. 8 ). In further embodiment these are followed by one or more coevolution steps.
  • the step of molecular directed evolution preferably comprises positively selecting for recombination on the intermediate target sites and/or negatively selecting against recombination on other target sites.
  • the selected candidates are preferably mutagenized so as to introduce mutations into the sequence for testing in the next round of selection.
  • Suitable methods of mutagenesis include in particular error-prone PCR, chemical mutagenesis, the use of specific mutator host strains, recursive ensemble mutagenesis, combinatorial cassette mutagenesis and DNA shuffling as well as a combination of these methods.
  • One or more, preferably, eight to forty, preferably fifteen cycles of molecular directed evolution are preferably be carried out, until DNA-recombining enzymes are evolved that are ac-tive on the intermediate target sites.
  • the molecular directed evolution employed is preferably a substrate-linked protein evolution, in particular as described in WO 2002044409 A2.
  • directed evolution strate-gies such as continuous evolution (WO2012088381 A2) are also applicable.
  • a further set of intermediate target sites is created by further adapting the sequences to the half sites of the target sites (selected in a1)), preferably by replacing one or more, preferably up to three nucleotides that differ by the nucleotides that occur in the corresponding half sites of the target site.
  • the sub steps of molecular directed evolution, shuffling and creating further sets of intermediate target sites is preferably performed until the intermediate target sites only differ in one to three, preferably one or two, nucleotides per half site from the half sites of the target sites (selected in a1)).
  • DNA recombining enzymes that induce a replacement (DRiGR) step b) is carried out in three sub-steps of molecular evolution.
  • the library or libraries are evolved as described before for DRiGD without an artificial sequence (thus without a donor vector) to select for recombinases that show at least some activity on the target site, thus DNA recombining enzymes that show at least excision activity.
  • This first step is preferably performed as described before firstly on a first set of intermediate target sites and then optionally on a second set of intermediate target sites and finally on the final target sites.
  • a second step the library or libraries of DNA recombining enzymes obtained as result of step i) are evolved using an artificial sequence with a positive selection marker, like an antibiotic resistance gene.
  • the artificial sequence is preferably a replication deficient donor vector.
  • a plasmid or linear sequence that does not carry an origin of replication that is active in the host cells is used (as exemplified in FIGS. 9 A- 9 C ).
  • the second step is preferably performed on the final target sites.
  • DNA recombining enzymes are selected that are capable to replace a sequence by DRiGR (which is a more complex reaction than a pure excision).
  • a replication deficient donor vector in combination with an antibiotic selection marker makes it possible to separate the first recombination reaction (integration) on the first target site, from the second recombination reaction (excision) on the second target site, necessary for a successful DRiGR reaction.
  • a third step molecular evolution is performed with the library or libraries of DNA recombining enzymes obtained as result of step ii) with an artificial sequence without a positive selection marker.
  • the artificial sequence is preferably a donor vector that carries a functional origin of replication that is active in the host cells used (as exemplified in FIG. 14 ).
  • the third step is preferably performed on the final target sites. Preferably 5 to 20 cycles, more preferably 8 to 15 cycles, of substrate linked molecular evolution are performed until enzymes are obtained that are active on the final target sites.
  • DNA recombining enzymes are selected that show a high replacement activity without the need of a positive selection marker and with similar recombination kinetics on target site 1 and target site 2. This is an important point as for many uses (in particular a medical use or also when generating multi cellular organism with a modified sequence) the presence of a positive selection marker (e. g. antibiotic resistance gene) is not a favorable option.
  • a positive selection marker e. g. antibiotic
  • This three-step method has been shown to be very efficient to select for enzymes that induce a replacement (DRiGR) in particular when combined with a vector that comprises one or several recognition sites for the same or in particular for two to three (or even up to five) dif-ferent restriction enzymes between the target sites.
  • DRiGR replacement dif-ferent restriction enzymes
  • vector refers to a nucleic acid molecule capable of transporting a nucleic acid to which it has been linked.
  • Vectors include, but are not limited to, nucleic acid molecules that are single-stranded, double-stranded, or partially double-stranded; nucleic acid molecules that comprise one or more free ends, no free ends (e.g. circular); nucleic acid molecules that comprise DNA, RNA, or both; and other varieties of polynucleotides known in the art.
  • plasmid refers to a circular double stranded DNA loop into which additional DNA segments can be inserted, such as by standard molecular cloning techniques.
  • viral vector Another type of vector is a viral vector, wherein virally-derived DNA or RNA sequences are present in the vector for packaging into a virus (e.g. retroviruses, replication defective retroviruses, adenoviruses, replication defective adenoviruses, and adeno-associated viruses—AAV).
  • Viral vectors also include polynucleotides carried by a virus for transfection into a host cell.
  • Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g. bacterial vectors having a bacterial origin of replication and episomal mammalian vectors).
  • Other vectors e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome.
  • vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as “expression vectors.”
  • Common expression vectors of utility in recombinant DNA techniques are often in the form of plasmids.
  • the expression vector is preferably a plasmid, a virus or an artificial chromosome.
  • Another object of the invention are the vectors or vector as used in the method.
  • Preferred expression vectors comprise:
  • a synthetic nucleic acid preferably a replication deficient plasmid
  • a positive selection marker lies in between the first and second intermediate target site.
  • the first and second intermediate target site preferably surround a positive selection marker, like an antibiotic resistance gene).
  • the method according to the invention for preparing DNA-recombining enzymes may comprise the further step of:
  • the invention comprises also the designer DNA-recombining enzymes obtained by the method according to the invention.
  • the inventors provide a solution for recombination-based genome editing that allows flexible and flawless alteration of genetic information.
  • the methods allow in particular for the generation of DNA-recombining enzymes to replace genetic mutations causing human diseases, or to delete predefined genetic material from cells.
  • the DNA-recombining enzymes according to the invention are used to alter a nucleotide sequence, in particular to repair a mutation or to delete a sequence from the genome or a sequence introduced by a virus.
  • the mutations that can be repaired according to the invention in particular comprise point mutations, frame shift mutations, deletions and insertions.
  • the DNA recombining enzyme can use target sequences naturally occurring in a genome, including the genome of a host cell, a host organ or a host organism or even the genome of a pathogen, like a virus.
  • An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (indels).
  • the invention comprises also a method to modify the genome by inducing site-specific recombination on naturally occurring target sites that are not identical.
  • the method comprises delivery of a DNA recombining enzyme according to the invention into the host, by either delivery of the protein itself, or introducing and expression of a nucleotide sequence encoding it, either from an mRNA or from a DNA template.
  • naturally occurring means that the target sites are not artificially introduced—there are natively present in the same genome.
  • Suitable host cells are in particular eukaryotic cells, including stem cells like hematopoietic stem cells, neuronal stem cells, adipose tissue derived stem cells, fetal stem cells, umbilical cord stem cells, induced pluripotent stem cells and embryonic stem cells.
  • stem cells like hematopoietic stem cells, neuronal stem cells, adipose tissue derived stem cells, fetal stem cells, umbilical cord stem cells, induced pluripotent stem cells and embryonic stem cells.
  • stem cells like hematopoietic stem cells, neuronal stem cells, adipose tissue derived stem cells, fetal stem cells, umbilical cord stem cells, induced pluripotent stem cells and embryonic stem cells.
  • human embryonic stem there are preferably not derived from the destruction of embryos. Further the modification of the human germline and human gametes as host cells are preferably excluded.
  • Suitable host organs for delivery of a DNA recombining enzyme according to the invention comprise any cell in the body, including bone marrow, the skin, muscles, the liver, the lungs, lymph node and the spleen.
  • the method according to the invention can be performed in vitro (outside of a living organism) or in vivo.
  • In vitro includes the manipulation of isolated cells and/or organs.
  • the invention provides a method of replacing or deleting a sequence of interest in a genome as described above.
  • the invention relates to the (preferably non-therapeutic) use of a DNA recombining enzyme according to the invention to create an cell or a (preferably non human) multicellular organism with a knock-out of sequence of interest (e. g. a gene or exon) or carrying a modified sequence (e.g. a transgene).
  • a DNA recombining enzyme e.g. a gene or exon
  • a modified sequence e.g. a transgene
  • the DNA recombining enzyme according to the invention or the genes encoding the enzyme according to the invention are introduced into a cell or organism by a delivery method.
  • the designer DNA-recombining enzyme according to the invention induces a site-specific recombination in a genome on two non-identical target sites that surround a sequence of interest.
  • DRiGD site-specific recombination for deletion
  • a synthetic sequence comprising a desired sequence (Donor DNA) is additionally introduced into the host.
  • the synthetic sequence comprises the desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site that are recognized by the DNA recombining enzyme according to the invention.
  • the invention provides a method of generating a model eukaryotic cell comprising a mutated or altered disease associated-gene or polynucleotide or a mutated or altered sequences, selected from signaling biochemical pathway-associated genes and polynucleotides. Examples of these genes and polynucleotides are listed below in table A to C.
  • Another application of the invention is to specifically alter a sequence of interest in a genome, in particular by introducing a mutation or introducing or replacing a nucleotide sequence, e. g. to create a model eukaryotic cell or an animal model, which has preferably a particular mutation that corresponds to a human disease and/or which bears a humanized sequence.
  • the method allows the targeted generation of animal models without inducing random insertions and deletions (indels) or other unwanted uncontrolled modification of genetic material.
  • the introduced mutation is associated with an increase in the risk of having or developing a disease.
  • the method according to the invention is used to create animal models, which are useful for biomedical research, e. g. as models for human diseases.
  • the invention is carried out in an animal it is preferably carried out for non-therapeutic use of the animal.
  • Suitable host organisms include invertebrates and vertebrates, particularly Bovidae, Drosophila melanogaster, Caenorhabditis elegans, Xenopus laevis , medaka, zebrafish, Mus musculus, Ratus norvegicus or embryos of these organisms.
  • plants and fungi as well as their cells can be used as hosts.
  • one or more vectors described herein are used to produce a non-human transgenic animal or transgenic plant.
  • the transgenic animal is a mammal, such as a mouse, rat, or rabbit.
  • the organism or subject is a plant.
  • the organism or subject or plant is algae.
  • Methods for producing transgenic plants and animals are known in the art, and generally begin with a method of cell transfection.
  • Transgenic animals are also provided, as are transgenic plants, especially crops and algae.
  • the transgenic animal or plant may be useful in applications outside of providing a disease model. These may include food or feed production through expression of, for instance, higher protein, carbohydrate, nutrient or vitamins levels than would normally be seen in the wildtype.
  • transgenic plants, especially pulses and tubers, and animals, especially mammals such as livestock (cows, sheep, goats and pigs), but also poultry and edible insects, are preferred.
  • a delivery vector for introducing and expression of the DNA recombining enzyme into a host a delivery vector can be used or other delivery methods, such as DNA, mRNA or protein packaged in liposomes or nanoparticles (e.g. as described by Wang M et al. (2016)), or electroporation ex vivo, or cell squeeze are also applicable.
  • delivery methods such as DNA, mRNA or protein packaged in liposomes or nanoparticles (e.g. as described by Wang M et al. (2016)), or electroporation ex vivo, or cell squeeze are also applicable.
  • the delivery method used depends on the host, host cell or target tissue in the host wherein the sequence of interest shall be altered.
  • a delivery vector used depends on the host, host cell or target tissue in the host wherein the sequence of interest shall be altered.
  • Suitable delivery vector are known by the person skilled in the art and include plasmids, artificial chromosomes, retroviral vectors, lentiviral vectors, spumavirus vectors and adenoviral vectors but also agrobacteria harboring shuttle vectors.
  • Advantageous delivery vectors include lentiviruses and adeno-associated viruses, and types of such vectors (in particular adeno associated viral vectors) can also be selected for targeting particular types of cells or tissue
  • the delivery vector preferably comprises a regulatory element.
  • regulatory element is intended to include promoters, enhancers, internal ribosomal entry sites (IRES), and other expression control elements (e.g. transcription termination signals, such as polyadenylation signals and poly-U sequences).
  • the regulatory element is a cellular, bacterial, a viral or a hybrid promoter, wherein said promoter is preferably a constitutive or inducible promoter.
  • the promoter is preferably selected depending on the host, host cell or target tissue in the host. Suitable promotors are also known by the person skilled in the art.
  • Preferred constitutive promoters are selected from the promoters of cytomegalovirus, Rous sarcoma virus, murine leukemia virus-related retroviruses, phosphoglycerokinase gene, murine spleen focus-forming virus or human elongation factor 1 alpha.
  • the delivery vector preferably contains also the donor sequence that comprises the two non-identical target sites recognized by the designer DNA-recombining enzyme according to the invention and whereas the target sites surround a desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site.
  • Donor DNA desired sequence
  • the donor sequence is provided on a separate nucleic acid molecule or vector (e. g. a replication deficient plasmid)
  • the invention comprises also nucleic acids or vectors encoding for a designer DNA-recombining enzyme according to the invention.
  • nucleic acids encoding for the monomers of a designer DNA-recombining enzyme according to the invention are one nucleic acid molecule or in one vector.
  • the monomers of the designer DNA-recombining enzymes are expressed under control of one promotor or other regulatory element.
  • the invention also comprises a method for transduction of a cell with
  • the donor sequence can be located on the expression or delivery vector or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • the donor sequence is provided at multiple copies per cell to increase the likelihood of replacement of the target sequence (see also FIG. 3 ).
  • the invention relates a set or kit comprising:
  • the donor sequence is either located on the same vector (encoding for the designer DNA-recombining enzyme according to the invention) or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • the desired sequence on the donor sequence is preferably a sequence that corresponds to the sequence of interest, but wherein the mutation is replaced by the wild type sequence (a gene or part thereof like an exon) or a functional variant thereof (e. g. a silent mutation or a mutation that increase the activity).
  • a further important part of the invention is the medical application of the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention.
  • sequence of interest is a “disease-associated” gene or polynuclotide, that is altered to cure the disease, or at least reduce the symptoms or to prolong life.
  • the invention relates to an in vitro method.
  • the invention is applied to isolated cells or organs as described above.
  • the invention in another aspect relates to a method of the treatment of subject, preferably a human or animal, comprising the step of applying a DNA recombining enzyme according to the invention, a nucleic acid or vector encoding for a designer DNA-recombining enzyme according to the invention or the set according to the invention.
  • Delivery methods and vectors are preferably selected as described above.
  • the subject to be treated carries a mutation in a nucleotide sequence (sequence of interest) that causes the subject to suffer from a disease, in particular a mutation that causes a reduced function or loss of function of the gene-product, e. g. a mutation in the factor 9 gene that causes hemophilia.
  • a nucleic acid or vector encoding it and a donor sequence carrying a desired sequence a wild type or other functional variant of the gene (or a part thereof—like an exon) the mutant part of the gene is replaced by the desired (functional) sequence.
  • the desired sequences in the donor sequence is flanked by the target sites for the DNA recombining enzyme.
  • the subject to be treated carries a nucleotide sequence (sequence of interest) that causes a disease or the inhibition reduces the symptoms thereof.
  • sequence of interest is deleted by applying the method according to the invention and delivery of the DNA recombining enzyme according to the invention, a nucleic acid or vector encoding it.
  • the sequence of interest is in particular a sequence of a pathogen (a microorganism like a bacteria, a virus or a parasite) or an oncogene or another gene, whereas the activity causes a disease or the inhibition of gene activity is known to reduce symptoms (like for example the gene encoding PCSK9).
  • the invention comprises a pharmaceutical composition
  • a pharmaceutical composition comprising a DNA recombining enzyme according to the invention, a nucleic acid or vector encoding for a designer DNA-recombining enzyme according to the invention or the set according to the invention as well as suitable carriers.
  • the sequence of interested targeted by a DNA recombining enzyme according to the invention can be any polynucleotide endogenous or exogenous to the eukaryotic cell.
  • the sequence of interested can be a polynucleotide residing in the nucleus or mitochondria of the eukaryotic cell or a virus integrated into the genome of the host cell or not.
  • the sequence of interested can be a sequence coding a gene product (e.g., a protein) or a noncoding sequence (e.g., an intron or a regulatory polynucleotide).
  • the sequence of interested targeted by a DNA recombining enzymes according to the invention may include a number of disease-associated genes and polynucleotides as well as signaling biochemical pathway-associated genes and polynucleotides.
  • a “disease-associated” gene or polynucleotide refers to any gene or polynucleotide which is yielding transcription or translation products at an abnormal level or in an abnormal form in cells derived from a disease-affected tissues compared with tissues or cells of a non disease control. It may be a gene that is expressed at an abnormally high level; it may be a gene that becomes expressed at an abnormally low level, where the altered expression correlates with the occurrence and/or progression of the disease.
  • a disease-associated gene also refers to a gene possessing mutation(s) or genetic variation that is directly responsible or is in linkage disequilibrium with a gene(s) that is responsible for the etiology of a disease.
  • the transcribed or translated products may be known or unknown and may be at a normal or abnormal level.
  • diseases-associated genes and polynucleotides are available from McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, Md.) and National Center for Biotechnology Information, National Library of Medicine (Bethesda, Md.), available on the World Wide Web.
  • sequence of interested targeted by a DNA recombining enzymes may include a number of disease-associated genes and polynucleotides as well as signaling biochemical pathway-associated genes and polynucleotides as listed in the US patent U.S. Pat. No. 8,697,359 B1, the contents of which is herein incorporated by reference in its entirety.
  • Examples of disease-associated genes and polynucleotides are listed in Tables A and B. Disease specific information is available from McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, Md.) and National Center for Biotechnology Information, National Library of Medicine (Bethesda, Md.), available on the World Wide Web. Examples of signaling biochemical pathway-associated genes and polynucleotides are listed in Table C.
  • genes and pathways can result in production of improper proteins or proteins in improper amounts which affect function.
  • genes, diseases and proteins are hereby incorporated by reference from U.S. Provisional application.
  • Such genes, proteins and pathways may be the sequence of interest targeted by a DNA recombining enzymes according to the invention.
  • Neoplasia PTEN A DISEASE/ DISORDER GENE(S) Neoplasia PTEN; ATM; ATR; EGFR; ERBB2; ERBB3; ERBB4; Notch1; Notch2; Notch3; Notch4; AKT; AKT2; AKT3; HIF; HIF1a; HIF3a; Met; HRG; Bcl2; PPAR alpha; PPAR gamma; WT1 (Wilms Tumor); FGF Receptor Family members (5 members: 1, 2, 3, 4, 5); CDKN2a; APC; RB (retinoblastoma); MEN1; VHL; BRCA1; BRCA2; AR (Androgen Receptor); TSG101; IGF; IGF Receptor; Igf1 (4 variants); Igf2 (3 variants); Igf 1 Receptor; Igf 2 Receptor; Bax; Bcl2; caspases family (9 members: 1, 2, 3, 4, 6, 7, 8,
  • APH-1 alpha and beta
  • Presenilin Psen1
  • nicastrin Ncstn
  • PEN-2 Disorders Other Prion-related Nos1; Parp1; Nat1; Nat2, Prp disorders ALS SOD1; ALS2; STEX; FUS; TARDBP
  • VEGF VEGF-a; VEGF-b; V EGF-c
  • Drug addiction Prkce (alcohol); Drd2; Drd4; ABAT (alcohol); GRIA2; Grm5; Grin1; Htr1b; Grin2a; Drd3; Pdyn; Gria1 (alcohol) Autism Mecp2; BZRAP1; MDGA2; Sema5A; Neurexin 1; Fragile X (FMR2 (AFF2); FXR1; FXR2; Mglur5) Alzheimer's E1; CHIP; UCH; UBB; Tau; LRP; PICALM; Clusterin; PS1; SO
  • BCL7A, BCL7 Cell dysregulation B-cell non-Hodgkin lymphoma
  • BCL7A, BCL7 Leukemia and oncology
  • Inflammation and AIDS KIR3DL1, NKAT3, NKB1, AMB11, KIR3DS1, IFNG, CXCL12, SDF1; immune related Autoimmune lymphoproliferative syndrome (TNFRSF6, APT1, FAS, CD95, diseases and ALPS1A); Combined immuno-deficiency, (IL2RG, SCIDX1, SCIDX, IMD4); disorders HIV-1 (CCL5, SCYA5, D17S136E, TCP228), HIV susceptibility or infection (IL10, CSIF, CMKBR2, CCR2, CMKBR5, CCCKR5 (CCR5)); Immuno-deficiencies (CD3E, CD3G, AICDA, AID, HIGM2, TNFRSF5, CD40, UNG, DGU, HIGM4, TNFSF5, CD40LG, HIGM1, IGM, FOXP3, IPEX, AIID, XPID, PIDX, TNFRSF14B, TACI); Inflammation (IL
  • Muscular/Skeletal Becker muscular dystrophy (DMD, BMD, MYF6), Duchenne Muscular Dystrophy diseases and (DMD, BMD); Emery-Dreifuss muscular dystrophy (LMNA, LMN1, EMD2, FPLD, disorders CMD1A, HGPS, LGMD1B, LMNA, LMN1, EMD2, FPLD, CMD1A); Facio-scapulohumeral muscular dystrophy (FSHMD1A, FSHD1A); Muscular dystrophy (FKRP, MDC1C, LGMD2I, LAMA2, LAMM, LARGE, KIAA0609, MDC1D, FCMD, TTID, MYOT, CAPN3, CANP3, DYSF, LGMD2B, SGCG, LGMD2C, DMDA1, SCG3, SGCA, ADL, DAG2, LGMD2D, DMDA2, SGCB, LGMD2E, SGCD, SGD, LGMD2
  • Neurological and ALS SOD1, ALS2, STEX, FUS, TARDBP, VEGF (VEGF-a, VEGF-b, VEGF-c); neuronal diseases Alzheimer disease (APP, AAA, CVAP, AD1, APOE, AD2, PSEN2, AD4, STM2, and disorders APBB2, FE65L1, NOS3, PLAU, URK, ACE, DCP1, ACE1, MPO, PACIP1, PAXIP1L, PTIP, A2M, BLMH, BMH, PSEN1, AD3); Autism (Mecp2, BZRAP1, MDGA2, Sema5A, Neurexin 1, GLO1, MECP2, RTT, PPMX, MRX16, MRX79, NLGN3, NLGN4, KIAA1260, AUTSX2); Fragile X Syndrome (FMR2, FXR1, FXR2, mGLUR5); Huntington's disease and disease like disorders (HD, IT15, PRNP, PRIP, JPH3,
  • Occular diseases Age-related macular degeneration (Aber, Ccl2, Cc2, cp (ceruloplasmin), Timp3, cathepsinD, and disorders Vldlr, Ccr2); Cataract (CRYAA, CRYA1, CRYBB2, CRYB2, PITX3, BFSP2, CP49, CP47, CRYAA, CRYA1, PAX6, AN2, MGDA, CRYBA1, CRYB1, CRYGC, CRYG3, CCL, LIM2, MP19, CRYGD, CRYG4, BFSP2, CP49, CP47, HSF4, CTM, HSF4, CTM, MIP, AQP0, CRYAB, CRYA2, CTPP2, CRYBB1, CRYGD, CRYG4, CRYBB2, CRYB2, CRYGC, CRYG3, CCL, CRYAA, CRYA1, GJA8, CX50, CAE1, GJA3, CX46, CZP3, CAE3, CCM1, CAM,
  • Embodiments of the invention also relate to methods and compositions related to knocking out genes, amplifying genes and repairing particular mutations, including point mutations, frame shift mutations, deletions or insertions.
  • the invention may be used to correct ocular defects that arise from several genetic mutations.
  • the genetic brain diseases may include but are not limited to Adrenoleukodystrophy, Agenesis of the Corpus Callosum, Aicardi Syndrome, Alpers' Disease.
  • the condition may be neoplasia. In some embodiments, where the condition is neoplasia, the genes to be targeted are any of those listed in Table A (in this case PTEN asn so forth). In some embodiments, the condition may be Age-related Macular Degeneration. In some embodiments, the condition may be a Schizophrenic Disorder. In some embodiments, the condition may be a Trinucleotide Repeat Disorder. In some embodiments, the condition may be Fragile X Syndrome. In some embodiments, the condition may be a Secretase Related Disorder. In some embodiments, the condition may be a Prion—related disorder. In some embodiments, the condition may be ALS. In some embodiments, the condition may be a drug addiction. In some embodiments, the condition may be Autism. In some embodiments, the condition may be Alzheimer's Disease. In some embodiments, the condition may be inflammation. In some embodiments, the condition may be Parkinson's Disease.
  • proteins associated with Parkinson's disease include but are not limited to ⁇ -synuclein, DJ-1, LRRK2, PINK1, Parkin, UCHL1, Synphilin-1, and NURR1.
  • addiction-related proteins may include ABAT for example.
  • inflammation-related proteins may include the monocyte chemoattractant protein-1 (MCP1) encoded by the Ccr2 gene, the C—C chemokine receptor type 5 (CCR5) encoded by the Ccr5 gene, the IgG receptor IIB (FCGR2b, also termed CD32) encoded by the Fcgr2b gene, or the Fc epsilon R1 g (FCER1g) protein encoded by the Fcerlg gene, for example.
  • MCP1 monocyte chemoattractant protein-1
  • CCR5 C—C chemokine receptor type 5
  • FCGR2b also termed CD32
  • FCER1g Fc epsilon R1 g
  • cardiovascular diseases associated proteins may include IL1B (interleukin 1, beta), XDH (xanthine dehydrogenase), TP53 (tumor protein p53), PTGIS (prostaglandin 12 (prostacyclin) synthase), MB (myoglobin), IL4 (interleukin 4), ANGPT1 (angiopoietin 1), ABCG8 (ATP-binding cassette, sub-family G (WHITE), member 8), or CTSK (cathepsin K), for example.
  • IL1B interleukin 1, beta
  • XDH xanthine dehydrogenase
  • TP53 tumor protein p53
  • PTGIS prostaglandin 12 (prostacyclin) synthase)
  • MB myoglobin
  • IL4 interleukin 4
  • ANGPT1 angiopoietin 1
  • ABCG8 ATP-binding cassette, sub-family G (WHITE), member 8
  • CTSK
  • Examples of Alzheimer's disease associated proteins may include the very low density lipoprotein receptor protein (VLDLR) encoded by the VLDLR gene, the ubiquitin-like modifier activating enzyme 1 (UBA1) encoded by the UBA1 gene, or the NEDD8-activating enzyme E1 catalytic subunit protein (UBE1C) encoded by the UBA3 gene, for example.
  • VLDLR very low density lipoprotein receptor protein
  • UBA1 ubiquitin-like modifier activating enzyme 1
  • UBE1C NEDD8-activating enzyme E1 catalytic subunit protein
  • proteins associated Autism Spectrum Disorder may include the benzodiazapine receptor (peripheral) associated protein 1 (BZRAP1) encoded by the BZRAP1 gene, the AF4/FMR2 family member 2 protein (AFF2) encoded by the AFF2 gene (also termed MFR2), the fragile X mental retardation autosomal homolog 1 protein (FXR1) encoded by the FXR1 gene, or the fragile X mental retardation autosomal homolog 2 protein (FXR2) encoded by the FXR2 gene, for example.
  • BZRAP1 benzodiazapine receptor (peripheral) associated protein 1
  • AFF2 AF4/FMR2 family member 2 protein
  • FXR1 fragile X mental retardation autosomal homolog 1 protein
  • FXR2 fragile X mental retardation autosomal homolog 2 protein
  • proteins associated Macular Degeneration may include the ATP-binding cassette, sub-family A (ABC1) member 4 protein (ABCA4) encoded by the ABCR gene, the apolipoprotein E protein (APOE) encoded by the APOE gene, or the chemokine (C—C motif) Ligand 2 protein (CCL2) encoded by the CCL2 gene, for example.
  • ABC1 sub-family A
  • APOE apolipoprotein E protein
  • CCL2 Ligand 2 protein
  • proteins associated Schizophrenia may include NRG1, ErbB4, CPLX1, TPH1, TPH2, NRXN1, GSK3A, BDNF, DISCI, GSK3B, and combinations thereof.
  • proteins involved in tumor suppression may include ATM (ataxia telangiectasia mutated), ATR (ataxia telangiectasia and Rad3 related), EGFR (epidermal growth factor receptor), ERBB2 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2), ERBB3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3), ERBB4 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 4), Notch 1, Notch2, Notch 3, or Notch 4, for example.
  • proteins associated with a secretase disorder may include PSENEN (presenilin enhancer 2 homolog ( C.
  • CTSB cathepsin B
  • PSEN1 presenilin 1
  • APP amphireactive protein
  • APH1B anterior pharynx defective 1 homolog B ( C. elegans )
  • PSEN2 presenilin 2 (Alzheimer disease 4)
  • BACE1 beta-site APP-cleaving enzyme 1
  • proteins associated with Amyotrophic Lateral Sclerosis may include SOD1 (superoxide dismutase 1), ALS2 (amyotrophic lateral sclerosis 2), FUS (fused in sarcoma), TARDBP (TAR DNA binding protein), VAGFA (vascular endothelial growth factor A), VAGFB (vascular endothelial growth factor B), and VAGFC (vascular endothelial growth factor C), and any combination thereof.
  • SOD1 superoxide dismutase 1
  • ALS2 amotrophic lateral sclerosis 2
  • FUS fused in sarcoma
  • TARDBP TAR DNA binding protein
  • VAGFA vascular endothelial growth factor A
  • VAGFB vascular endothelial growth factor B
  • VAGFC vascular endothelial growth factor C
  • proteins associated with prion diseases may include SODI (superoxide dismutase 1), ALS2 (amyotrophic lateral sclerosis 2), FUS (fused in sarcoma), TARDBP (TAR DNA binding protein), VAGFA (vascular endothelial growth factor A), VAGFB (vascular endothelial growth factor B), and VAGFC (vascular endothelial growth factor C), and any combination thereof.
  • proteins related to neurodegenerative conditions in prion disorders may include A2M (Alpha-2-Macroglobulin), AATF (Apoptosis antagonizing transcription factor), ACPP (Acid phosphatase prostate), ACTA2 (Actin alpha 2 smooth muscle aorta), ADAM22 (ADAM metallopeptidase domain), ADORA3 (Adenosine A3 receptor), or ADRA1D (Alpha-1D adrenergic receptor for Alpha-1D adrenoreceptor), for example.
  • A2M Alpha-2-Macroglobulin
  • AATF Apoptosis antagonizing transcription factor
  • ACPP Acid phosphatase prostate
  • ACTA2 Actin alpha 2 smooth muscle aorta
  • ADAM22 ADAM metallopeptidase domain
  • ADORA3 Adosine A3 receptor
  • ADRA1D Alpha-1D adrenergic receptor for Alpha-1D adrenoreceptor
  • proteins associated with Immunodeficiency may include A2M [alpha macroglobulin]; AANAT [arylalkylamine N-acetyltransferase]; ABCA1 [ATP-binding cassette, sub-family A (ABC1), member 1]; ABCA2 [ATP-binding cassette, sub-family A (ABC1), member 2]; or ABCA3 [ATP-binding cassette, sub-family A (ABC1), member 3]; for example.
  • proteins associated with Trinucleotide Repeat Disorders include AR (androgen receptor), FMR1 (fragile X mental retardation 1), HTT (huntingtin), or DMPK (dystrophia myotonica-protein kinase), FXN (frataxin), ATXN2 (ataxin 2). for example.
  • proteins associated with Neurotransmission Disorders include SST (somatostatin), NOS1 (nitric oxide synthase 1 (neuronal)), ADRA2A (adrenergic, alpha-2A-, receptor), ADRA2C (adrenergic, alpha-2C-, receptor), TACR1 (tachykinin receptor 1), or HTR2c (5-hydroxytryptamine (serotonin) receptor 2C), for example.
  • neurodevelopmental-associated sequences include A2BP1 [ataxin 2-binding protein 1], AADAT [aminoadipate aminotransferase], AANAT [arylalkylamine N-acetyltransferase], ABAT [4-aminobutyrate aminotransferase], ABCA1 [ATP-binding cassette, sub-family A (ABC1), member 1], or ABCA13 [ATP-binding cassette, sub-family A (ABC1), member 13], for example.
  • A2BP1 ataxin 2-binding protein 1
  • AADAT aminoadipate aminotransferase
  • AANAT arylalkylamine N-acetyltransferase
  • ABAT 4-aminobutyrate aminotransferase
  • ABCA1 ATP-binding cassette, sub-family A (ABC1), member 1
  • ABCA13 ATP-binding cassette, sub-family A (ABC1), member 13
  • preferred conditions treatable with the present invention include may be selected from: Aicardi-Goutines Syndrome; Alexander Disease; Allan-Herndon-Dudley Syndrome; POLG-Related Disorders; Alpha-Mannosidosis (Type II and III); Alström Syndrome; Angelman; Syndrome; Ataxia-Telangiectasia; Neuronal Ceroid-Lipofuscinoses; Beta-Thalassemia; Bilateral Optic Atrophy and (Infantile) Optic Atrophy Type 1; Retinoblastoma (bilateral); Canavan Disease; Cerebrooculofacioskeletal Syndrome 1 [COFS1]; Cerebrotendinous Xanthomatosis; Cornelia de Lange Syndrome; MAPT-Related Disorders; Genetic Prion Diseases; Dravet Syndrome; Early-Onset Familial Alzheimer Disease; Friedreich Ataxia [FRDA]; Fryns Syndrome; Fucosidosis; Fukuyama Congenital Muscular Dystrophy; Galactosialidosis; Gau
  • the present system can be used to target any polynucleotide sequence of interest.
  • Some examples of conditions or diseases that might be usefully treated using the present system are listed above and examples of genes currently associated with those conditions are also provided there. However, the genes exemplified are not exhaustive.
  • the designer DNA-recombining enzyme according to the invention replaces a mutation in exon 8 of the F9 gene that is responsible for hemophilia B.
  • Exon 8 is frequently mutated in severe forms of hemophilia B and mutations that occur in the population are known (www.factorix.org).
  • the F9 designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 1 (Rec F9-1) or a sequence with a least 90% sequence identity, more preferably 95 sequence identity to SEQ ID NO. 1 and a monomer with the sequence according to SEQ ID NO. 2 (Rec F9-2) or a sequence with a least 80% sequence identity, preferably 90 sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 2.
  • An object of the invention are also designer DNA-recombining enzyme comprising the sequence according to SEQ ID NO. 1 (Rec F9-1) or SEQ ID NO. 2 (Rec F9-2) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 1 or 2.
  • SEQ ID ID NO. 1 (Rec F9-1): MSNLQTLHQN LSALLVXAXS DXARKNLMDX FRDRQAFSEH TWXVLLSVCR SWAAWCKLNX 60 RKWFPAEXXD VRDYLLHLQA XGLXVNTIXQ HLXQLNMLHR RSGLPRPGDS NAVSLVMRRI 120 RKENVDAGER VKQALAFERX DFDQVRSLME NSDRCQDIRN LAFLGXAYNT LLRISEXARI 180 RXXDIXRTDG GRMLIHIGRT KTLVSXAGVE KALSLRVTRL VXRWXSVSGV ADDPNNXLXC 240 RVRRNGVAXP SATSQLSTXX LQGXFAAAHR LIYGARDXSG QRYXTWSGHS ARVGAARDMA 300 RAGVSIAEIM QAGGWTTXES VMNYIRNLDS ETGAMVRLLE DXD 343
  • X17 is any amino acid, preferably D or G X19 is any amino acid, preferably T or A X22 is any amino acid, preferably G or E X30 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A X43 is positively charged amino acid, preferably K or R, X60 is any amino acid, preferably N or D X68 is any amino acid, preferably P or S X69 is a positively or negatively charged amino acid, preferably selected form D, E, K or R, more preferably E or K, X81 is a polar amino acid, preferably selected form S, T, C, K or R, more preferably C or R, X84 is any amino acid, preferably A or T X89 is any amino acid, preferably L or Q X93 is a small amino acid, preferably selected form C, S and A, more preferably C or A, X140 is any amino acid, preferably I or T X166 is an unpolar amino acid, preferably
  • sequences according SEQ ID No 1 are selected from sequences according to SEQ ID No 28, SEQ ID No 3 and SEQ ID No 5 and sequences with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID No. 28, SEQ ID No 3 or SEQ ID No 5.
  • SEQ ID ID NO. 2 (Rec F9-2): MSXLXTLXQN LSAXLXDXXX XEARKNLMDV XRDRQAFSXH TWRVLLSVCR SWAAWCELNN 60 RKWFPAEPED VRDYLLHLQX RGLXVNT1QQ HLXQLNXLHR RSGLPRPGDS NAVSLVMRRI 120 RKENXDAGER VXQALAFERT DFDQVRSLME NSDRCQDIRN LAFLGVAYNT LLRISEIARI 180 RXXDIXRTDG GRMLXHIGRT KTLVSXAGVE KALSLXVTKL VERWISVSGV ADDPNNYLFC 240 RVRRNGVAXP SAXSQLSTXX LQGXFXAAHR LIXGAXDXSG QRYLTWSGHS ARVGAARDMA 300 RAGVSXAEIM QAGGWTTVES VMNYXRNLDS ETGAMVRLLE DGD 343
  • X3 is any amino acid, preferably K or N X5 is any amino acid, preferably P or Q X8 is any amino acid, preferably H or T X14 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or L, X16 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A X18 is small amino acid, preferably A or G X19 is any amino acid, preferably T or A X20 is a polar amino acid, preferably selected from S, T, N or Q, more preferably S or N X21 is any amino acid, preferably D or G X31 is an unpolar amino acid, preferably selected form I, L, V and F, more preferably L or F X39 is a positively or negatively charged amino acid, preferably selected form D, E, K or R, more preferably K or E, X80 is any amino acid, preferably T or A X84 is any amino acid, preferably T or A X
  • Preferred sequences according SEQ ID No 2 are selected from sequences according to SEQ ID no. 29, SEQ ID No 4 and SEQ ID No 6 and sequences with a least 85% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID No. 29, SEQ ID No 4 or SEQ ID No 6.
  • Preferred pairs of monomers to form the F9 designer DNA-recombining enzyme according to the invention bear the amino acids sequences according to SEQ 28 and 29, and in particular SEQ 3 and 4 as well as SEQ 5 and 6.
  • the F9 designer DNA-recombining enzyme comprises a sequence according to SEQ ID No. 36 (Rec F9-3) or a sequence with a least 90% sequence identity, preferably 95%, more preferably 98% sequence identity to SEQ ID NO. 36.
  • the F9 designer DNA-recombining enzyme is preferably a homotetramer.
  • the F9 designer DNA-recombining enzyme according to the invention has been obtained using the method according to the invention described herein and recognizes the asymmetric loxF9-A and loxF9-A target sites according SEQ ID No 7 and SEQ ID No 8:
  • the invention comprises a nucleic acid selected from SEQ ID NO. 7 (loxF9a) and SEQ ID NO. 8 (loxF9b) or a sequence with a least 90% sequence identity, preferably a least 95 sequence identity, more preferably at least 99% sequence identity, to SEQ ID NO. 7 or SEQ ID NO. 8 or a nucleic acid sequence reverse complementary to one of these sequences and their use as target site for a DNA recombining enzyme, preferably a F9 designer DNA recombining enzyme according to the invention.
  • these nucleic acids according to the invention have a length of 30 to 40 nucleotides, preferably 32 to 36, more preferably 34 nucleotides.
  • the invention also comprises nucleic acids and vectors encoding for the F9 designer DNA-recombining enzyme according to the invention.
  • the invention also comprises a method for transduction of a cell with:
  • the donor sequence can be located on the expression or delivery vector or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • the donor sequence is provided at multiple copies per cell to increase the likelihood of replacement of the target sequence (see also FIG. 3 and exemplified in FIG. 11 ).
  • the invention further comprises a method for replacing a (non-or malfunctional) mutant exon 8 of the F9 gene in a cell, by introducing into a cell:
  • the invention also comprises a kit comprising
  • the defective F9 exon 8 sequence is replaced by a donor sequence carrying the hyperactive Padua mutation (R338L) (PMID: 23197580).
  • SEQ ID No. 26 wild type exon 8
  • SEQ ID No. 27 exon 8 with Padua mutation
  • the donor sequence can be located on the vector encoding for the F9 designer DNA-recombing enzyme (s. FIG. 11 for an example) or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • the donor sequence is provided in the above-mentioned methods and the kit at multiple copies per cell (e.g. on an AAV vector) to increase the likelihood of replacement of the target sequence (see also FIG. 11 for an example)).
  • the designer DNA-recombining enzyme deletes a sequence on the human chromosome 7.
  • This designer DNA-recombining enzyme is further referred to as Hex designer DNA-recombining enzyme and recognizes the asymmetric Hex1 and Hex2 target sites according SEQ ID No 40 and SEQ ID No 41:
  • the designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 46 (Hex-R-#7) or a sequence with a least 90% sequence identity, more preferably 95% sequence identity to SEQ ID NO. 46 and a monomer with the sequence according to SEQ ID NO. 47 (Hex-L-#7) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 47.
  • the designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 48 (Hex-R-#30) or a sequence with a least 90% sequence identity, more preferably 95 sequence identity to SEQ ID NO. 48 and a monomer with the sequence according to SEQ ID NO. 49 (Hex-L-#30) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 49.
  • the invention also comprises nucleic acids and vectors encoding for the Hex designer DNA-recombining enzyme according to the invention.
  • the invention includes the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention for use in medicine, in particular for the treatment of a disease or condition associated with one of the above-mentioned disease-associated genes or polynucleotides or the above mentioned signaling biochemical pathway-associated genes and polynucleotides.
  • the invention includes the preparation of a medicament for the treatment of a disease or condition associated with one of the above-mentioned disease genes or polynucleotides or the above mentioned signaling biochemical pathway-associated genes and polynucleotides comprising the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention or using a method according the invention.
  • FIG. 1 shows a general scheme of a preferred Designer DNA Recombining enzyme induced Gene Replacement (DRiGR) according to the invention. Different monomers R1-4 of DNA recombining enzymes and their respective DNA binding sequences loxR1-loxR4 (half sites of target sites) are shown. The site of interest in the genome (frowny face) is replaced by a desired sequence (smiley).
  • DRiGR Designation of Gene Replacement
  • FIG. 2 shows a general scheme of a Designer DNA Recombining enzyme induced Gene Deletion (DRiGD). Again, different monomers R1-4 of DNA recombining enzymes binding at their respective DNA binding sequences loxR1-loxR4 are shown in the upper scheme. Here the target sites bear each identical 8 bp spacers. The site of interest in the genome (frowny face) is here cut out.
  • DRiGD Designer DNA Recombining enzyme induced Gene Deletion
  • FIG. 3 illustrates that if the donor vector is in excess to the genomic DNA, Gene Replacement induced by the DNA recombining enzyme is driven towards the replacement of the genomic DNA with the donor vector DNA.
  • the donor vector is present in multiple copies in the nucleus of the target cell, such as in AAV vectors, which results in efficient repair of cellular mutations.
  • FIG. 4 demonstrates a general method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (Duo-SLIDE DRiGR) that delivers a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with two libraries of DNA recombining enzymes.
  • Duo-SLIDE DRiGR novel directed evolution strategy
  • FIG. 15 shows for three different restriction enzymes or the respective restriction sites.
  • P1 and P2 stand for two different PCR primers or the respective primer binding sites.
  • FIG. 5 demonstrates a similar method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (QuSLiDE DRiGR) that delivers two pairs of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites.
  • each monomer is directed to one half site of one target site (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with four libraries of DNA recombining enzymes.
  • R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .
  • the gene defect is repaired in cells (s. FIG. 1 ).
  • FIG. 6 demonstrates a general method to obtain a DNA Recombining enzyme for DRiGD by applying a novel directed evolution strategy (Duo-SLIDE DRiGD) that delivers a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with two libraries of DNA recombining enzymes.
  • Duo-SLIDE DRiGD a novel directed evolution strategy that delivers a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with two libraries of DNA recombining enzymes.
  • R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .
  • FIG. 7 demonstrates a similar method to obtain a DNA Recombining enzyme for DRiGD by applying a novel directed evolution strategy (QuSLiDE DRiGD)) that delivers two pairs of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites.
  • each monomer is directed to one half site of one target site (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with four libraries of DNA recombining enzymes.
  • R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .
  • This set of monomers allows deleting a site of interest in a Genome (s. FIG. 2 ).
  • FIG. 8 shows a method know in the state of the art to obtain a tailored recombinase that works one target sites by substrate-linked protein evolution (SLiPE) (Buchholz F and Stewart AF, 2001).
  • SLiPE substrate-linked protein evolution
  • FIGS. 9 A- 9 C show the application of the invention to replace a mutation in the exon 8 of the F9 gene by Duo-SLIDE DRiGR.
  • FIG. 9 A shows the schematic presentation of exon 8 and flanking sequence of the F9 gene.
  • the two selected target sequences (loxF9a—SEQ ID No. 3 and loxF9b—SEQ ID No. 4) are indicated by triangles (black and white) and the nucleotide sequence and the chromosomal positions are shown.
  • FIG. 9 B illustrates the method to obtain the two recombinase monomers F9-1 (SEQ ID No. 1, 3, 5 and 28) and F9-2 (SEQ ID No. 2, 4, 6 and 29) and the replacement reaction.
  • FIG. 9 C shows an agarose gel demonstrating the restriction pattern for pDuoF9 (source) (lane 1) and pDuoF9 (product) (lane 2) after digestion with NdeI and SacI.
  • M molecular marker.
  • FIG. 10 shows an exemplary evolution process of adapting the libraries of DNA recombining enzymes to a given target site—here applied to the loxF9a and loxF9b target sites.
  • DNA recombining enzymes that are active on the loxF9a site were obtained by designing two symmetric intermediate sites AL and AR and performing 20 or 17 cycles of SLiPE (s. FIG. 8 ). After combining and shuffling the libraries of DNA recombining enzymes obtained 108 cycles of SLiPE were performed on the asymmetric loxF9a site (F9A). Agarose gels of indicated generation cycles are shown, with the line with two triangles indicating the non-recombined band and the line with one triangle showing the size of the recombined band. The grey triangle below the gel pictures indicates the reduced amount of L-arabinose added to the growth medium.
  • FIG. 11 illustrates the use the F9 designer DNA recombining enzymes in a therapeutic setting
  • both F9-1 and F9-2 coding sequences are cloned into a delivery vector (such as an adeno-associated viral vector) together with the donor sequence that contains loxF9a and loxF9b flanking the wild-type, or Padua mutation (R338L) of exon 8 of the F9 gene. Delivery of such a vector into target cells in multiple copies replaces the inactivating mutation in the genome.
  • a delivery vector such as an adeno-associated viral vector
  • FIG. 12 shows the result of a screen of the human PCSK9 Gene on Chromosome 1 for 8 bp repeats.
  • the exons of the PCSK9 Gene are indicated by bold boxes.
  • the 8 bp repeats are separated by at least 150 bp and no more than 2 kb apart and lie in potential target sites (indicated by vertical lines) with a length of 34 bp, that were screened not occurring elsewhere in the genome.
  • the sequences of interests between the potential target sites are indicated by horizontal lines.
  • FIG. 13 shows the result of a similar screen of the genome of the human papilloma virus 16 (HPV16) for 8 bp repeats that are separated by at least 150 bp and no more than 2 kb apart and lie in potential target sites of 34 bp, that were screened not to occur in the human genome.
  • the sequences encoding for the viral proteins are indicated in bold boxes with arrows (greater-than signs).
  • FIG. 14 demonstrates a different general method to obtain a DNA Recombining enzyme for DRiGR used in example 3 step 3 by applying a novel directed evolution strategy (SLIDE DRiGR 2.0) that delivers a library of DNA recombining enzymes that recombines two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting.
  • R1, R2, R3, R4 and R5 stand for five different restriction enzymes or the respective restriction sites.
  • P3 and P4 stand for two different PCR primers or the respective primer binding sites.
  • FIG. 15 highlights the steps of the novel directed evolution strategy (SLIDE DRiGR) used in example 3 step 3.
  • pF9 source
  • pDonor are transformed into bacteria. Only if the expression of the recombinases is induced, growth of colonies resistant to the antibiotic can be observed.
  • sub step 2 the plasmid DNA of the obtained colonies is digested using restriction enzymes R1-3. This will enrich for clones which have undergone successful DRIGR and result in a pure preparation of pF9 (product). The purity of the plasmid preparation is verified with a restriction digest using the enzymes XhoI and XmaI, which shows the unique restriction pattern of pDuoF9 (product). Additionally, DNA sequencing reveals the correct sequence of pF9 (product).
  • FIG. 16 demonstrates a different general method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (Duo-SLIDE DRiGR 2.0) that delivers one or a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with one or two libraries of DNA recombining enzymes.
  • R1, R2, R3, R4 and R5 stand for five different restriction enzymes or the respective restriction sites.
  • P3 and P4 stand for two different PCR primers or the respective primer binding sites.
  • FIG. 17 shows alignment between Cre (state of the art), a recombinase obtained after step 1 of example 3 (R #1), a recombinase obtained after step 2 of example (3R #7-B5), and a recombinase obtained after step 3 of example 3 (F9-3).
  • the alignment shows how additional mutations have arisen during later stages of evolution.
  • FIG. 18 shows a general scheme of the method of the invention, exemplified by the DRiGD method and steps of the invention.
  • FIG. 19 and FIG. 20 show a more detailed scheme of the method of the invention, exemplified by the DRiGD method and applied to the human genome.
  • FIGS. 21 A- 21 C illustrate the DRIGD method of the invention of applied to a target site on the human chromosome 7—further described in example 5.
  • FIG. 21 A shows the target sites: Hex 1 and Hex 2 are the target sites used for excision—HexL, HexR, HexR1 and HexR2 are intermediate target sites.
  • FIG. 21 B shows the evolution of specific recombinases for the Hex1 and Hex2 target sites.
  • FIG. 21 C shows recombination efficiency of two clones (Clone #7 and Clone #30) obtained.
  • FIG. 22 shows the precise excision of a sequence from a plasmid substrate using the recombinases obtained in example 5.
  • FIG. 12 shows the results for all 309 possible target sites for DNA recombining enzymes in the human gene PCSK9.
  • FIG. 13 shows the results for all 176 possible target sites for DNA recombining enzymes in the human papilloma virus 16 (HPV16).
  • the target sequences (loxF9a—SEQ ID No. 7 and loxF9b—SEQ ID No. 8) were selected by comparing sequences in the human genome with half sites of previously identified target sites (LoxP, LoxH, LoxM7, LoxM5, loxLTR, LoxBTR) of DNA recombining enzymes:
  • each library was evolved towards recombining intermediate target sites (AL, AR, BLS, BLRS, BL, BR) and finally to recombining one of the two target sites, loxF9a or loxF9b.
  • New mutations were introduced through error-prone PCR and DNA-shuffling and selective pressure was further regulated via the expression level of the recombinases.
  • Active recombinases were selected by their ability to recombine the respective target site to delete the sequence flanked by the target sites from the vector (s. FIG. 8 ).
  • Recombinase coding sequences from these two libraries were then cloned in the vector backbone pDuoF9 (source) (SEQ ID No. 21, s. FIGS. 9 A- 9 C ).
  • the two recombinase libraries are expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together.
  • the size of this library exceeded 100.000 clones.
  • E. coli XL1-Blue cells were transformed with 1 ng pDuoF9 (source) library and grown overnight in 200 ml LB medium containing 15 ⁇ g/ml chloramphenicol.
  • 1 ml of the overnight culture was used to inoculate 100 ml of fresh medium and grown for 2 h.
  • the culture was split into 2 ⁇ 50 ml and L-arabinose was added to a final concentration of 50 ⁇ g/ml to induce recombinase expression in one of the cultures.
  • the cells were put on ice and prepared for electroporation as described before (Sambrook and Russell, 2001).
  • the electrocompetent cells were resuspended in 200 ⁇ l water, and 50 ⁇ l of the cell suspension was immediately used for transformation of 0.4 ng pDonor at 1700 V. The bacteria were then allowed to recover for 2 h in SOC medium before the entire suspension was plated on LB agar plates containing 15 ⁇ g/ml kanamycin.
  • the plasmid DNA was isolated from the culture. To enrich for clones that had undergone successful DRiGR, the DNA was digested with enzymes cleaving any non-pDuoF9 (product)—negative selection—and re-transformed. To this end, 500 ng of plasmid preparation were digested with 1 ⁇ l of each NdeI, AvrII and PspXI. After 3 h incubation at 37° C. DNA was precipitated and resuspended in 50 ⁇ l water. 1 ⁇ l of this was used for transformation of 50 ⁇ l electrocompetent E. coli XL1-Blue cells. The transformed cells were grown in 220 ml LB medium containing 15 ⁇ g/ml kanamycin—positive selection-.
  • the recombinase libraries were amplified by PCR using primers P1 and P2 (SEQ ID No.23 and 24) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI.
  • the isolated recombinase libraries were then ligated back into pDuoF9 (source) to start another cycle of DRiGR.
  • Three rounds of Duo-SLIDE DRIGR (s. FIG. 10 ) were performed to enrich for recombinases that can efficiently carry out DRiGR on the target sites loxF9a and LoxF9b.
  • each library was evolved as described in example 2 towards recombining intermediate target sites (loxF9-AL, AR, BLS, BLRS, BL, BR—s. example 2) and finally to recombining one of the two target sites, loxF9a or loxF9b.
  • New mutations were introduced through error-prone PCR and DNA-shuffling and selective pressure was further regulated via the expression level of the recombinases. Active recombinases were selected by their ability to recombine the respective target site to delete the sequence flanked by the target sites from the vector (s. FIG. 8 ).
  • a single recombinase library was evolved to carry out DRiGR following a selection scheme with the replication deficient pDonor (s. FIGS. 9 A- 9 C ).
  • This involved cloning the recombinase coding sequences from the libraries generated in step 1 in the vector backbone pF9 (source) ( pDuoF9 SEQ ID No. 21).
  • source pDuoF9 SEQ ID No. 21
  • this vector one to four recombinase libraries can be expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together.
  • the size of this library exceeded 100.000 clones.
  • E. coli XL1-Blue cells were transformed with 1 ng pDuoF9 (source) library and grown overnight in 200 ml LB medium containing 15 ⁇ g/ml chloramphenicol.
  • 1 ml of the overnight culture was used to inoculate 100 ml of fresh medium and grown for 2 h.
  • the culture was split into 2 ⁇ 50 ml and L-arabinose was added to a final concentration between 1-200 ⁇ g/ml to induce recombinase expression in one of the cultures.
  • the cells were put on ice and prepared for electroporation as described before (Sambrook and Russell, 2001).
  • the electrocompetent cells were resuspended in 200 ⁇ l water, and 200 ⁇ l of the cell suspension was immediately used for transformation of 80 ng pDonor at 1700 V.
  • the bacteria were then allowed to recover for 2 h in SOC medium before the entire suspension was used to incoculate 200 ml LB medium containing 15 ⁇ g/ml chloramphenicol, 5 ⁇ g/ml kanamycin and 1-200 ⁇ g/ml L-arabinose.
  • a small part of the suspension was plated on agar plates to estimate the library size.
  • the plasmid DNA was isolated from the liquid culture. To enrich for clones that had undergone successful DRiGR, the DNA was digested with enzymes cleaving any non-pDuoF9 (product)—negative selection—and re-transformed. To this end, 500 ng of plasmid preparation were digested with 1 ⁇ l of each NdeI, AvrII, PspXI and FspI.
  • the plasmid DNA was isolated from the culture, and 500 ng of DNA was digested with 1 ⁇ l of each NdeI and AvrII.
  • the recombinase libraries were amplified by error-prone PCR using primers P1 # and P2 #(SEQ ID No. 30 and 31) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI.
  • the isolated recombinase libraries were then ligated back into pDuoF9 (source) to start another cycle of DRiGR. After cycles 3 and 8 DNA shuffling was carried out as described before (Buchholz and Stewart, 2001). Eleven rounds of Duo-SLIDE DRIGR were performed to enrich for recombinases that can efficiently carry out DRiGR on the target sites loxF9a and loxF9b.
  • a single recombinase library was evolved to carry out DRiGR 2.0 following a different selection scheme (s. FIG. 14 ) with the high-copy number plasmid pDonor-ex8 (s. FIG. 14 , SEQ ID 37) without antibiotic selection and without separating integration from resolution of the donor vector.
  • This involved cloning the recombinase coding sequences from the library generated in step 2 in the vector backbone pF9 (source) ( pDuoF9 (source) SEQ ID No. 21).
  • the library was then transformed into electrocompetent E. coli XL1-Blue cells which were already carrying pDonor-ex8.
  • the transformed cells were grown in 100 ml LB medium containing 15 ⁇ g/ml chloramphenicol, 5 ⁇ g/ml kanamycin and 1-200 ⁇ g/ml L-arabinose.
  • the size of this library exceeded 100.000 clones.
  • the plasmid DNA was isolated from the culture, and 1000 ng of DNA was digested with 1 ⁇ l of each NdeI, AwlI, PspXI (restriction enzymes R1, R2 and R3—s. FIGS. 14 and 15 ) and the exonuclease RecBCD.
  • the recombinase libraries were amplified by error-prone PCR using primers P3 and P4 (SEQ ID No. 32 and 33) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI.
  • the isolated recombinase libraries were then ligated back into pF9 (source) and transformed into electrocompetent E.
  • coli XL1-Blue cells carrying pDonor-ex8.
  • the transformed cells were grown in 100 ml LB medium containing 15 ⁇ g/ml chloramphenicol, 5 ⁇ g/ml kanamycin and 1-200 ⁇ g/ml L-arabinose and the next cycle thus began. Every three cycles DNA shuffling was carried out as described before (Buchholz and Stewart, 2001). Eleven rounds of SLIDE DRIGR (s. FIG. 14 ) were performed to enrich for recombinases that can efficiently carry out DRIGR on the target sites loxF9a and LoxF9b without antibiotic selection and without separating integration from resolution of the donor vector.
  • DRGR efficiency recombinase efficiency
  • Example Selection Recombinase DRIGR Step scheme obtained SEQ ID No. efficiency 1 SLIDE R#1 34 0.5% (s. FIG. 8) 2 Duo-SLIDE R#7-B5: 35 12.3% DRIGR (s. FIG. 9A-9C) 3 SLIDE-DIGR F9-3 36 90% 2.0 (s. FIG. 14)
  • both F9-1 and F9-2 coding sequences are cloned into a delivery vector (such as an adeno-associated viral vector) together with the donor sequence that contains loxF9a and loxF9a flanking the wild-type, or Padua mutation (R338L) of exon 8 of the F9 gene. Delivery of such a vector into target cells in multiple copies replaces the inactivating mutation in the genome (s. FIG. 11 ).
  • a delivery vector such as an adeno-associated viral vector
  • both libraries obtained on HexR and HexL were combined in order to recombine the final target sites Hex1 and Hex2. Therefore, the libraries (library size was bigger than 100.000 recombinases) were cloned into the same expression plasmid and were co-expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together (s. FIG. 6 pDUO-SLiDE DRiGD). The plasmids carrying both libraries were then transformed into XL-1 blue E. coli cells. After recovering the cells in 1 ml of SOC medium for one hour, 10 ⁇ l was plated on a LB agar plate with 15 ⁇ g/ml chloramphenicol.
  • the next day 32 clones were picked and cultured in 500 ⁇ l of LB with 25 ⁇ g/ml chloramphenicol for 8 h. Next, 250 ⁇ l pre-culture was used to inoculate 2 ⁇ 5 ml of LB with 25 ⁇ g/ml chloramphenicol. In one of the 5 ml cultures 10 ⁇ g/ml L-arabinose was added to induce the expression of the recombinase dimer. The next day plasmid DNA was isolated and digested with SacI (R2) and SbfI (R3) to estimate the recombination efficiency in the induced and uninduced sample.
  • SacI SacI
  • SbfI SbfI
  • the target sites and the intermediate target sites and primers used are listed in the following table:
  • Hex target sites SEQ-ID Hex1 TACACAGTGTATATTGATTTTTATCAAATGCCTT 40 Hex2 TACACAATGTATATTGATTTTTATCAAATGCCTT 41 HexL TACACAGTGTATATTGATTTTTATACATTGTGTA 42 HexR AAGGCATTTGATATTGATTTTTATCAAATGCCTT 43 HexR1 AAGACATTTTATATTGATTTTTATAAAATGTCTT 44 HexR2 AACGCATTGGATATTGATTTTTATCCAATGCGTT 45 P1# CGGCGTCACACTTTGCTATG 30 P2# AAGGGAATAAGGGCGACACG 31

Abstract

The invention provides methods and means for specifically altering the DNA sequence in a genome, in particular for genome editing by deleting or replacing a sequence of interest. Advantageously, the invention uses two non-identical sequences naturally occurring in a genome as target sites two which DNA-recombining enzymes are generated. The invention is in particular useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (in-dels).

Description

    RELATED APPLICATIONS
  • This application is a division of U.S. patent application Ser. No. 16/622,937, filed Dec. 16, 2019, which is a 35 U.S.C. § 371 filing of International Patent Application No. PCT/EP2018/065881, filed Jun. 14, 2018, which claims priority to European Patent Application No. 17175895.6, filed Jun. 14, 2017, the entire disclosures of which are hereby incorporated herein by reference.
    • SEQUENCE LISTING
  • The content of the electronically submitted sequence listing in XML format (Name: 734613_TUD9-001USDIV_ST26-2023-02-20.xml; Size: 143,251 bytes; and Date of Creation: Feb. 20, 2023) is incorporated herein by reference in its entirety.
  • The invention provides methods and means for specifically altering the DNA sequence in a genome. Provided are vectors and methods to generate designer DNA recombining en-zymes. The invention is useful for medicine, in particular to repair a mutation in a genome or to delete predefined genetic material from cells or tissue and to cure diseases. Further, the invention is useful for biological and biomedical research (e. g. to create animal models).
  • Many genetic mutations that cause human diseases have been identified over the last decades. Recent breakthroughs in the field of genome editing now provide a genuine opportunity to establish innovative approaches to repair DNA lesions to replace, engineer or regenerate malfunctioning cells in vitro, or in the body.
  • However, most of the recently developed genome editing technologies such as zinc finger nucleases (e. g. US20150093802 A1), TALENs (e. g. WO2014134412 A1) and CRISPR/Cas9 (e. g. U.S. Pat. No. 8,697,359 B1) introduce double stranded DNA breaks at a target locus as the first step to gene correction. These breaks are subsequently repaired by one of the cell intrinsic DNA repair mechanisms, typically inducing an abundance of random insertions and deletions (indels) at the target locus. Ideally, therapeutic genome editing should, however, be efficient and specific, without the introduction of indels.
  • DNA recombining enzymes, in particular site-specific recombinase (SSR) systems, allow precise manipulation of DNA without triggering endogenous DNA repair pathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processed DNA in vivo. Furthermore, SSR systems, such as Cre/loxP (EP 0 2200 009 B1) have found widespread use in model organisms, demonstrating that they can be safe, even when expressed lifelong in animals. SSRs work as tetramers on their target sites and depending on the orientation of these sites, recombination can lead to a variety of outcomes. It is known that the amino acid sequence of Cre can be modified in order to obtain novel site-specific recombinases (Buchholz F and Stewart AF 2001).
  • Other SSR systems know in the art include the Flp/FRT system (WO 1999025841 A1 and U.S. Pat. No. 6,774,279 B2), the Dre/rox system (U.S. Pat. No. 7,422,889 B2 and U.S. Pat. No. 7,915,037 B2), the VCre/VloxP system and the sCre/SloxP system (WO 2010/143606 A1), as well as the Vika/vox, the Nigri/nox and the Panto/pox systems (WO 2014/016248 A1).
  • Naturally occurring DNA recombining enzymes, in particular site-specific recombinase (SSR) systems (such tyrosine-type SSRs), generally consist of four identical monomers. In general they recognize two identical and symmetric, palindromic target sites, which consist each of two 13 nucleotide long half sites separated by an asymmetric frequently 8 nucleotide long spacer. Depending on the number and relative orientation of the target sites the DNA recombining enzyme either causes a deletion, an insertion, an inversion or a replacement of genetic content.
  • A bottleneck for using DNA recombining enzymes in therapeutic genome editing has been the limited number of sequences they can recombine.
  • In WO2008083931A1 this limitation was addressed to some extend by the directed molecular evolution of tailored recombinases (Tre 1.0) that uses sequences in the long terminal repeat (LTR) of HIV as recognition sites (loxLTR Tre 1.0). Further developments of this approach using asymmetric target sites were described in WO2011147590 A2 (Tre 3.0) and WO2016034553 A1 (Tre 3.1 and uTre/Brec1) as well as the publication Karpinski J et al 2016 (Bred). However, in all cases this approach has limited utility, because the exact recognition site with a length of 34 base pairs has to be present twice in the genome (such as in the LTR of an integrated HI provirus) for the system to work.
  • WO2009007982A1 describes nucleic acid sequences located in the LTR of HIV-1 as potential target sites for DNA recombining enzymes.
  • US2009/0217400 A1 describes an enzyme and a method for recombination at asymmetric loxP-M7 target sites. Further, Zhang, C et al. (2015) and US 20170058297 A1 describe an obligate heterotetramer complex of two Cre mutants that recognize asymmetric loxP-M7 target sites. However, these recognition sites have to be introduced artificially into the genome.
  • US 20040003435 A1 describes a method for targeted insertion of a sequence of interest into a plant said method comprising introducing a transfer cassette comprising said nucleotide sequence of interest flanked by or comprising non-identical recognition sites, which are modified FRT sites that can still be recognized by the wild-type FLP recombinase.
  • Taken together the application of the recombinase systems known in the state of the art is limited by either the fact that two recognition sites have to be introduced artificially into the genome or that the sequence of interest in the genome has to be flanked by two identical sequences with a length of 34 base pairs.
  • Therefore, currently straightforward genome editing approaches rely on programmable nucleases, such as RNA-guided nucleases like CRISPR/Cas9 (e. g. U.S. Pat. No. 8,657,359 B1) as the specificity of these enzymes for a target site is defined by the guide RNA, which can be versatility adapted to a sequence of interest.
  • However, as the nucleases systems need the cell intrinsic DNA repair mechanisms they typically induce an abundance of random insertions and deletions (indels) at the target locus.
  • The ideal genome editing or gene therapy will replace the defective gene with a normal allele or delete a defective gene at its natural location without further modification of the genome. Thus, the ideal genome editing should be efficient and specific, without the introduction of indels.
  • An objective of the invention is therefore to provide a method to alter a nucleotide sequence in a genome that overcomes the disadvantages of the nuclease genome editing approaches and which is at the same time versatile adaptable to a sequence of interest.
  • The invention is based on the finding that two identical sequences with a length of 34 base pairs (the length of a usual target site of a DNA recombining enzyme) are rarely found in a genome.
  • The invention starts with the realization of the inventors that the only restriction to achieve excision recombination for Designer DNA-recombining enzymes is the conservation of the spacer sequence, normally 8 bp long (see FIG. 17 for an example). Because this sequence has to be identical for recombination to occur, it sets a constraint for sequences that can be recombined. However, a bioinformatics analysis of different genomes revealed that direct repeats of 8 bp length are frequently occurring in viral, prokaryotic and eukaryotic genomes, including the human genome (see FIG. 18, 19 for an example).).
  • Based on this finding the inventors realized that through a combination of different monomers of DNA-recombining enzymes evolved to target different target sites in the genome, precise genome editing becomes possible without engaging cellular repair pathways. Thus, a combination of monomers of DNA-recombining enzymes generated via directed evolution, rational design, or a combination thereof can be generated to virtually recombine any sequence of interest in a genome. In case the recombination shall result in a deletion of a sequence two identical sequences with a length of approximately 8 base pairs are needed as spacer sequence in the genome. However, in case the recombination shall result in a replacement of a sequence the spacer sequences are preferably not identical in the genome
      • the identical spacer sequence are in this case provided with the desired sequence (donor DNA) that shall replace the sequence of interest on an artificial sequence.
  • By combining monomers of different DNA-recombining enzymes the invention overcomes the limitation of known DNA-recombining enzymes in respect of the target sites they can recombine and provides designer DNA recombining enzymes that are useful for genome editing that can recognize target sequences occurring naturally in a genome.
  • In a first aspect of the invention the invention provides designer DNA-recombining enzymes that are capable to induce a site-specific DNA recombination to alter a nucleotide sequence in a genome by recombining two non-identical target sequences (preferably naturally) occurring in the genome and a method for preparing the designer DNA-recombining enzymes.
  • The designer DNA-recombining enzymes according to the invention advantageously does not need target sites that are artificially introduced in the genome and further does not require identical sequences with a length over 8 nucleotides (in particular not 34 nucleotides) to be present, like long terminal repeats (LTRs).
  • Non-identical target sequences mean in the context of this invention that the target sites differ in at least two nucleotides. Advantageously the target sites may differ even more, in particular in at least three nucleotides. In embodiments of the invention the target sites differ in at least four nucleotides or even eight nucleotides. Advantageously at least 20% or even more preferred 30% of the nucleotides and up to 70% or even up to 90% of the nucleotides in the target sites can differ, which means that the target site may have a sequence identity of only 10 to 80%, preferably 30 to 90%. Each target site comprises a first half site (preferably 10 to 20 nucleotides) and a second half site (preferably 10 to 20 nucleotides) separated by a spacer sequence (preferably 5 to 12 nucleotides). Advantageously one or even both target sites may be asymmetric, meaning that the half sites of the target site are not reverse-complementary to each other. Advantageously at least 20% or even more preferred 30% of the nucleotides and up to 70% or even up to 90% of the nucleotides in the half sites can differ, which means that all the half sites may have a sequence identity of only 10 to 80%, preferably 30 to 90%. In one embodiment, the differences in the target sites lie only in the half sites and the spacer sequences are identical to induce deletion of the sequence of interest. In another embodiment the two spacer sequences are also different to enable replacement of the sequence of interest by providing a synthetic sequence comprising two sequences that are identical to the target sites occurring in the genome surrounding a donor sequence that shall replace the sequence of interest.
  • In a preferred embodiment the designer DNA-recombining enzymes according to the invention comprises one or two or up to four different monomers. In one embodiment the designer DNA-recombining enzyme comprises four different monomers and each monomer recognizes a different half site: However, it is also possible that the designer DNA-recombining enzyme comprises two or three different monomers whereas one or two monomers recognize an asymmetric target site. It is even possible that the designer DNA-recombining enzyme comprises four identical monomers whereas the designer DNA-recombining enzyme recognize two different asymmetric target sites. Different monomers according to the invention means that the monomers differ in at least 2%, in particular at least 4%, preferably at least 8% of the amino acid residues. Advantageously up to 10% or even up to 30% of the amino acid residues may differ, which means that the monomers may have a sequence identity of only 70 to 98%, preferably 90 to 96% or up to 92%.
  • Thus the DNA-recombining enzyme according to the invention is either a homotetramer or a heterotetramer comprising two or up to four different monomers.
  • In a preferred embodiment of the heterotetramer the monomer-monomer interfaces are genetically modified in such a way that only distinct monomers can form tetramers (e. g. as described in the publication Zhang et al. 2015 and in and US 20170058297 A1). By this modification an obligate heterotetramer is formed excluding tetramers comprising only one monomer.
  • The sequence of interest is a nucleotide sequence to be altered. It is preferably a sequence comprising mutation. According to the invention the term mutation includes all kind of genetic or chromosomal aberrations, in particular point mutations, frame shift mutations, translocations, duplications, deletions or insertions.
  • Alternatively, the sequence of interest is a sequence that shall be inactivated, in particular a sequence of a pathogen (a microorganism like a bacteria, a virus or a parasite) or an oncogene or another gene, whereas the activity causes a disease or the inhibition of gene activity is known to reduce symptoms. One example is the gene encoding PCSK9 (Proprotein convertase subtilisin/kexin type 9), where it is known that inhibition reduces cholesterol levels and blood concentration of low density lipoprotein particles (LDL).
  • In case the sequence of interest is an insertion, a duplication or a sequence that shall be inactivated (e. g. a viral sequence), the recombination induced by the DNA-recombining enzyme according to the invention preferably results in a deletion of the inserted sequence or the sequence that shall be inactivated. This deletion—further also referred to as Designer DNA Recombining enzyme induced Gene Deletion (DRiGD)—is achieved by two identical spacer sequences in the two target sites (s. also FIG. 2 for an illustrative example).
  • Alternatively, the recombination induced by the DNA-recombining enzyme according to the invention results in a replacement of the sequence of interest by a desired sequence. This replacement—further also referred to as Designer DNA Recombining enzyme induced Gene Replacement (DRiGR)—is achieved by providing a synthetic donor sequence comprising the first target site and the second target site, whereas the first and second target site surround the desired sequence. By replacing the sequence of interested by the desired sequence, it is in principle possible to cure any mutation including point mutations, nonsense mutations, frame shift mutations, duplications, and even deletions and insertions (s. also FIG. 1 for an illustrative example).
  • Advantageously, the DNA recombining enzymes according to the invention allow precise genome editing without triggering endogenous DNA repair pathways and possess the unique ability to fulfill both cleavage and immediate resealing of the processed DNA in vivo.
  • The term DNA recombining enzyme comprises every enzyme that is capable to induce a site-specific DNA recombination event, preferably a recombinase or integrase, in particular selected from enzymes that carry out a topoisomerase-like reaction, such as serine or tyrosine recombinase families. In one embodiment the known DNA-recombining enzymes whose target sequences are used in step a) and upon which molecular directed evolution is applied in steps (b) are selected from Cre, Dre, VCre, sCre, FLP, Tre (including Tre 1.0 to Tre 3.1 and Bred), Vika, Nigri and Panto.
  • The term “designer” DNA recombining enzyme refers to the facts that by the invention DNA recombining enzymes are generated to a given target site and that the DNA recombining enzymes do not occur in nature.
  • In one aspect the invention relates to a method for identifying sequences that are potential target sites for DNA-recombining enzymes that are capable to induce a site-specific DNA recombination of a sequence of interest in a genome, including the steps of:
      • i. Screening the genome or a part thereof comprising the sequence of interest for two sequences that are potential spacer sequences. These potential spacer sequences have a length of at least 5 bp, preferably at least 7 bp, more preferably 8 bp and preferably up to 12 more preferably up to 10 bp. One of the potential spacer sequence lies upstream of the sequence of interest and the other potential spacer sequence lies downstream of the sequence of interest. The two sequences have preferably a maxi-mum distance of 100 kb, more preferably 10 kb and even more preferably 2 kb and preferably a minimum distance of 150 bp.
      • ii. Identifying (Defining) potential target sites: The potential spacer sequences (obtained in step i.) as well as the sequences surrounding it form the potential target sites. For each potential spacer sequence (obtained in step i.) the neighboring nucleotides, preferably 10 to 20 nucleotides, more preferably 12 to 15 nucleotides, most preferably 13 nucleotides, on one side thereof form the potential first half site and the neighboring nucleotides, preferably 10 to 20 nucleotides, more preferably 12 to 15 nucleotides, on the other side form the potential second half site. Thus, both potential half sites and the spacer sequence in between form a potential target site.
      • iii. The potential target sites identified in step ii. are preferably further screened to select for potential target sequences that do not occur (elsewhere) in the genome of the host to insure a sequence specific recombination, in particular a deletion.
  • This method is in particular suitable to screen for identifying sequences that are potential target sites for DNA-recombining enzymes that induce a deletion (DRiGD) of a sequence of interest in a genome. In this case, the genome is screened in step i. for two identical sequences that are potential spacer sequences. This method is also illustrated by the example in FIG. 18 .
  • In one embodiment the sequence of interest to be deleted is a sequence in the genome of the host, e. g. an oncogene or another gene that shall be inactivated. Thus the genome screened in step i. is the genome of the host itself (or a part thereof comprising the sequence of interest), e. g. the human genome or part of a human chromosome. In this case in step iii. the potential target sequences are preferably screened not to occur elsewhere (thus only around the sequence of interest) in the genome of the host to insure a sequence specific deletion.
  • In another embodiment the sequence of interest to be deleted is a sequence in the genome of a pathogen, e. g. a virus. Here, step i. is performed on the genome of the pathogen and step iii. on the genome of the host, which is infected by the pathogen. In this case in step iii. the potential target sequences are preferably screened to occur not in the genome of the host (thus only in the genome of the pathogen) to insure a sequence specific deletion.
  • In a further preferred step iv. target sequences are selected from the list of potential target sequences obtained in step ii or iii. that comprise half sites that have each a homology to a half site of a known target site of a DNA-recombining enzyme. Homology means that a certain number of nucleotide positions bears identical bases. Preferably each half site has at least 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to a half site of a known target sites of a DNA-recombining enzyme. Further, the half sites preferably a have a homology between each other. Preferably, each half site has at least
  • 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to the other half site of the same target sites and/or the half sites of the other target site. The homology between target half-sites increases the chance that a monomer can be generated that is active on both half-sites and therefore the number of monomers necessary for recombination is reduced.
  • The selection of step iii. to insure a sequence specific deletion can be performed either before or after step iv.
  • Preferably during or before step i. the coordinates of the sequence of interest to be inactivated (preferably a gene or an exon) is determined in the genome. Search windows of 150 bp to 10 kb kB fragments covering the sequence of interest and surrounding sequences are generated. Fragments overlapping with other genes are removed. For all other fragments, potential identical spacer sequences—thus spacer sequences that are repeated at least once are identified.
  • In one embodiment spacer sequences are selected before step ii. that surround the sequence of interest (e. g. an exon of the gene to be inactivated or at least overlap with the sequence of interest (e. g. the exon). Alternatively, potential target sites are identified as described in step ii and subsequently pairs of target sites are selected that surround the sequence of interest (e. g. an exon of the gene to be inactivated) or at least overlap with the sequence of interest (e. g. the exon).
  • In another embodiment the method is used for identifying sequences that are potential target sites for DNA-recombining enzymes that induce a replacement (DRiGR) of a sequence of interest in a genome. In this case, the genome is screened in step i. for two sequences that are potential spacer sequences and are not identical. In this case, the potential spacer sequences differ in at least 30% to 100% preferably at least 50% of the nucleotides Thus, in this case the potential spacer sequences preferably have a sequence identity of 0% to 50 or at least below 70%.
  • In a further preferred embodiment sequences that are potential target sites for DNA-recombining enzymes that induce a replacement (DRiGR) of a sequence of interest in a genome are identified by a method including the steps of:
      • i. Screening the genome or a part thereof comprising the sequence of interest for two sequences that are potential target sites. These potential target sites comprise a first half site and a second half site with each 10 to 20 nucleotides, more preferably 12 to 15 nucleotides, separated by a spacer sequence with a length of at least 5 bp, preferably at least 7 bp, more preferably 8 bp and preferably up to 12 more preferably up to 10 bp. One of the potential target site lies upstream of the sequence of interest and the other potential target site lies downstream of the sequence of interest. The two sequences have preferably a maximum distance of 100 kb, more preferably 10 kb and even more preferably 2 kb and preferably a minimum distance of 150 bp. To enable a replacement the spacer sequences are chosen to be not identical. The spacer sequences differ in at least 30% to 100% preferably at least 50% of the nucleotides Thus, the spacer sequences preferably have a sequence identity of 0% to 50% or at least below 70%.
      • ii. The potential target sites identified in step i. are preferably further screened to select for potential target sequences that do not occur (elsewhere) in the genome of the host to insure a sequence specific replacement (with a donor sequence, see below).
  • In a further preferred step iii. target sequences are selected from the list of potential target sequences obtained in step i or ii. that comprise half sites that have each a homology to a half site of a known target site of a DNA-recombining enzyme. Homology means that a certain number of nucleotide positions bears identical bases. Preferably each half site has at least 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to a half site of a known target sites of a DNA-recombining enzyme.
  • The selection of step ii. to insure a sequence specific replacement can be performed either before or after step iii.
  • Preferably during or before step i. the coordinates of the sequence of the sequence of interest (preferably a gene or exon) to be replaced is determined in the genome. Search windows of 150 bp to 10 kb kB fragments covering the sequence of interest and surrounding sequences are generated. Fragments overlapping with other genes are removed. For all other fragments, potential target sites are identified.
  • In one embodiment spacer sequences are selected that surround the sequence of interest (e. g. an exon of the gene to be replaced) or at least overlap with the sequence of interest (e. g. the exon).
  • The method for preparing the designer DNA-recombining enzymes according to the invention enzymes that are capable to induce a site-specific DNA recombination to alter a nucleotide sequence in a genome by recombining two target sequences naturally occurring in the genome (Designer Recombinase-induced Gene Replacement or Deletion), comprises the following steps:
      • a) Selecting a nucleotide sequence (preferably with a length of 30 to 40 base pairs) up stream of the nucleotide sequence to be altered (site of interest) as first target site and a nucleotide sequence (preferably with a length of 30 to 40 base pairs) down-stream of the nucleotide sequence to be altered as second target site, whereas the sequences of the target sites are not identical. The term not identical or non-identical target sequences is defined as above. Advantageously, one or both target sites are asymmetric, as symmetric sites are hardly found in the genome. The target sites each comprise a first half site and a second half site (as defined above, preferably 10 to 20 nucleotides) separated by a spacer sequence (as defined above, preferably 5 to 12 nucleotides). The target sequences are preferably selected to comprise half sites that have each a homology to a half site of a known target site of a DNA-recombining enzyme. Homology means that a certain number of nucleotide positions bears identical bases. Preferably each half site has at least 10%, preferably at least 20%, prefera-bly at least 30% identical nucleotide positions to a half site of a known target sites of a DNA-recombining enzyme. Further, as explained above the half sites preferably a have a homology between each other. Preferably, each half site has at least 10%, preferably at least 20%, preferably at least 30% identical nucleotide positions to the other half site of the same target sites and/or the half sites of the other target site.
      • b) Applying molecular directed evolution on at least one library, preferably at least two and preferably up to four libraries, of DNA-recombining enzymes using a nucleic acid comprising the first target site and the second target site as selected in a) as substrate,
        until at least one designer DNA-recombining enzyme is obtained (by molecular directed evolution or a combination of molecular directed evolution and rational design) that is active on the first target site and the second target site as selected in a1).
  • The designer DNA-recombining enzyme may be selected to target any pair of target sequences and consequently any sequence of interested flanked by these target sites. The target sequences are naturally occurring sequences within a genome of a host (e. g. a cell or organism) or in the genome of a pathogen, like a virus. “Naturally occurring sequences within a genome” means in the context of the invention that the target sequences are not artificially introduced and that both non-identical sequences occur natively (naturally) in the same genome.
  • Genome is defined as all the genetic material of an organism or pathogen. It generally consists of DNA (or RNA in RNA viruses). The genome includes the genes (the coding regions ore exons), the noncoding DNA (like introns and regulatory sequences) and the genetic material of the mitochondria and chloroplasts.
  • Preferred target sequences include those that are unique in the target genome of a host cell or organism. As some organism have multiple copies of chromosomes, unique means in this context that they occur only once per set of chromosome.
  • The inventors have surprisingly found (see example F9-3, which can recombine both loxF9a and loxF9b) that one DNA-recombining enzyme can be used to recombine two different target sites in case all the half sites differ between each other in 7 to 9 of 13 positions.
  • In general, if all the half sites differ between each other in not more than 7 positions, preferably not more than 5 positions, more preferably not more than 4 positions. one library of DNA-recombining enzymes can be used to evolve an enzyme that can recombine both target sites, in particular in a DRiGR reaction (see FIGS. 15 and 16 and Table on page 63 for an example).
  • In another case the half sites show a pairwise difference of a maximum of 7, preferably 5, more preferably 4 mismatches—thus both target sites show a maximum of 7, preferably 5 or more preferably 4 mismatches between the half sites. In this case 2 libraries of DNA-recombining enzymes are used to evolve an enzyme that can recombine both target sites.
  • In a further case only one target site shows a pair of half sites with a maximum of 7, preferably 5, more preferably 4 mismatches—thus the other target site differs in more than 7, preferably 5, more preferably 4 mismatches and also the half sites of the other target site show more than 7, preferably 5, more preferably 4 mismatches compared to each other. In this case 3 or 4 libraries of DNA-recombining enzymes are used to evolve an enzyme that can recombine both target sites.
  • The library or libraries of DNA-recombining enzymes used in step b) are evolved to obtain one monomer of DNA-recombining enzymes or co-evolved to obtain at least two monomers of DNA-recombining enzymes that work together on the pair of target sites. In case two or more libraries of DNA-recombining enzymes used in step b) they are preferably operationally linked, preferably on one expression vector. In case two or more libraries of DNA-recombining enzymes are used, the libraries are preferably expressed under the control of the same regulatory elements, preferably the same promoter.
  • Preferably, one or two to four libraries of DNA-recombining enzymes are used in step b) for coevolution. In particular preferred embodiments two or four libraries of DNA-recombining enzymes are used in step b).
  • In case more than one library is used then the different libraries of DNA-recombining enzymes used differ in respect of the target site specificity.
  • In case two libraries of DNA-recombining enzymes are used the DNA-recombining enzymes in each library advantageously recognize different asymmetric target sites—target sites composed of two different half sites.
  • In case four libraries of DNA-recombining enzymes are used the DNA-recombining enzymes in each library preferably recognizes each a different half site of an asymmetric target site. Thus, they only recombine the target site, when co-expressed as heterotetramer.
  • In a preferred embodiment, the coevolution in step b) is performed using an expression vector that encodes for the library of DNA-recombining enzymes or the at least two different libraries of DNA-recombining enzymes and comprising the first and second target site. In particular, preferred embodiments the expression vector encodes for two or four libraries of DNA-recombining enzymes.
  • In a preferred embodiment of the vector a negative selection marker lies in between the first and second target site. Thus, the first and second target site, preferably surround a negative selection marker. Thus, in case a recombination occurs the sequence with the negative selection marker is cleaved out and no negative selection occurs. However, if no recombination occurs the negative selection marker is active.
  • The negative selection marker is in a preferred embodiment a recognition site for a restriction enzyme. Preferably a unique recognition site—meaning a recognition site for a restriction enzyme that occurs only once in the vector. By the incubation with the restriction enzyme expression vectors that have not undergone recombination are linearized. The linearized expression vectors are negative selected, e. g. by choosing a primer pair for a PCR that only amplifies the non-linearized plasmid (as exemplified in FIGS. 4 to 7 ) and/or by digesting the linearized DNA by an exonuclease.
  • In a preferred embodiment the negative selection comprises more than one recognition site for a restriction enzyme, preferably two or three (or even up to five) recognition sites for the same or in particular for two to three (or even up to five) different restriction enzymes.
  • This significantly enhance the efficiency of linearization of the expression vectors that have not undergone recombination. To further enhance the selection for enzymes that result in a replacement an exonuclease is preferably added to digest the linearized DNA.
  • The substrate nucleic acid comprising the first target site and the second target site (preferably surrounding a negative selection marker) is preferably situated on the same vector as the DNA encoding the library of DNA-recombining enzymes or the at least two different libraries of DNA-recombining enzymes (as exemplified in FIGS. 4 and 7 ).
  • In an embodiment, the method comprises also the transduction of a cell with the expression vector as defined above and expression of the DNA-recombining enzyme. Alternatively, a cell-free expression system is used.
  • In case the DNA-recombining enzyme according to the invention shall result in a deletion (DRiGD), preferably no further DNA substrates or vectors are used for one recombination reaction. A successful recombination cleaves out the negative selection marker and the plasmid is amplified—preferably by PCR (as exemplified in FIGS. 6 and 7 —pDuoSLiDE-DRiGD, pQuSLiDE-DRiGD). As described above in this case the first and second target sequences comprises identical spacer sequences (s. also FIG. 2 ).
  • The method for identifying sequences that are potential target sites for DNA-recombining enzymes described above is preferably performed when or before selecting the target sites in step a) of the method for preparing designer DNA-recombining enzymes for deletion or replacement.
  • In case the DNA-recombining enzyme according to the invention shall result in a replacement (DRiGR) a synthetic sequence comprising the first target site and the second target site is used in addition to the substrate. As described above in this case the first and second target sequences comprise different spacer sequences to avoid a deletion to occur. Consequently, the recombination is shifted versus replacement by recombining the identical target sequences on the substrate (on the vector encoding the libraries) and the synthetic sequence. Here the negative selection marker is replaced by recombining with the synthetic sequence.
  • In a preferred embodiment the synthetic sequence is a replication deficient plasmid (s. also FIGS. 4 and 5 for examples). However, in an alternative embodiment a linear DNA strand is used. In another preferred embodiment the synthetic sequences are on a high-copy number plasmid (s. FIG. 14 for an example).
  • To further select for DNA-recombining enzyme that result in a replacement the synthetic sequence preferably comprises a positive selection marker between the first and second target. In case a recombination occurs the sequence with the positive selection marker will be inserted into the expression vector and expression vectors bearing the positive selection marker can be positively selected. In one embodiment the positive selection marker is an antibiotic-resistance gene. Thus, after transduction of the vector into cells the cells compris-ing vectors with the positive selection marker can be easily selected.
  • In one embodiment (for DRiGR) the vector bears a negative selection marker between the first and second target site and the synthetic sequence additionally comprises a positive selection marker between the first and second target site.
  • Other negative and positive selection markers are known to a person skilled in the art. Further applicable negative selection markers include gene encoding enzymes that encode a gene encoding a toxin or genes that convert a prodrug into a toxic drug, like URA3 (converting 5-fluoroorotic acid into the toxic compound 5-fluorouracil) or viral thymidine kinases (making the host sensitive to ganciclovir selection). Further applicable positive selection markers are genes encoding for enzymes that can complement an auxotrophy.
  • One step of molecular evolution preferably comprises at least one selection step and at least one step of mutagenesis. In order that the selected molecules “evolve” between selection steps, the selected candidates are preferably mutagenized so as to introduce mutations into the sequence for testing in the next round of selection. Suitable methods of mutagenesis are known to those of skill in the art and include in particular error-prone PCR, chemical mutagenesis, the use of specific mutator host strains, recursive ensemble mutagenesis, combinatorial cassette mutagenesis and DNA shuffling as well as a combination of these methods.
  • One or more, preferably, five to fifty, preferably eight to thirty, cycles of co-evolution are preferably be carried out, until DNA-recombining enzymes are evolved that are active on the target sites.
  • In some embodiments of the invention, the co-evolution step b) can be directly performed on existing libraries of DNA-recombining enzymes.
  • In a preferred embodiment, the method of the invention comprises the following further steps to obtain the two libraries of DNA-recombining enzymes used in step b). Here step a) is further referred to as step a1)
    • a2) Identifying four sequences that are half sites of known target sites of DNA-recombining enzymes, whereas the first sequence is homologous to the first half site of the first target site (selected according to a1)), the second sequence is homologous to the second half site of the first target site (selected according to a1)), the third sequence is homologous to the first half site of the second target site (selected according to a1)), the fourth sequence is homologous to the second half site of the second target site (selected according to a1)),
  • Selecting a1) and Identifying a2) are preferably performed simultaneously in one step a), e. g. by a computer program, like the SeLOX algorithm (Surendranath, V. et al. (2010). Preferably the sequences up- and down-stream of the site of interest are compared with known target sites of DNA-recombining enzymes and a target sequence wherein each half site has a certain homology to a half site of a known target site of a DNA-recombining enzyme is selected as described in a1). The half sites of the known target sites identified in this way are the ones mentioned in a2). The degree of homology is preferably as mentioned in a1).
  • Step a3):
  • In this step preferably intermediate target sites are generated that are a mix between the half sites identified in step a2) and the target sites as selected in step a1).
  • The intermediate target sites are preferably generated by determining the nucleotides within the first to fourth sequences (as defined in a2)) that differ from the target sites selected in a1) and replacing one or more, preferably up to three nucleotides that differ by the nucleotides that occur in the corresponding half sites of the target site.
  • In one embodiment for separated molecular directed evolution of DNA-recombining enzymes on each intermediate target site the intermediate target sites are symmetric, thus they comprise identical or reverse complementary half sites. In this embodiment preferably a set of four intermediate target sites is generated—one intermediate target site for each half site of the target sites selected in a1).
  • In another embodiment for coevolution of DNA-recombining enzymes a set of preferably two asymmetric intermediate target sites are generated. The first intermediate first target site comprises one sequence that corresponds to the first sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the first half site of the first target site (as selected in step a1)) and a sequence that corresponds to the second sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the second half site of the first target site (as selected in a1)). The second intermediate target site that comprises a sequence that corresponds to the third sequence (as defined in a2)) in which nucleotides are replaced by nucleotides occurring in the first half site of the second target site (as selected in step a1)) and a sequence that corresponds to the fourth sequence (as de-fined in a2)) in which nucleotides are replaced by nucleotides occurring in the second half site of the second target site (as selected in step a1)).
  • Step a4):
  • Applying molecular directed evolution on DNA-recombining enzymes recognizing the sequences defined in step a2) using a nucleic acid comprising the intermediate first target site and/or the intermediate second target site as substrate.
  • In one embodiment the nucleic acid comprises both the intermediate first target site and the intermediate second target and a direct evolution of DNA-recombining enzymes is performed on both intermediate target sites (direct coevolution). The coevolution is preferably performed as described above for step b).
  • In a preferred embodiment the first rounds of molecular directed evolution are performed as a separated molecular directed evolution of DNA-recombining enzymes (separate evolution) for each intermediate target site (e g. as depicted in FIG. 8 ). In further embodiment these are followed by one or more coevolution steps.
  • The step of molecular directed evolution preferably comprises positively selecting for recombination on the intermediate target sites and/or negatively selecting against recombination on other target sites.
  • In order that the selected molecules “evolve” between selection steps, the selected candidates are preferably mutagenized so as to introduce mutations into the sequence for testing in the next round of selection. Suitable methods of mutagenesis are known to those of skill in the art and include in particular error-prone PCR, chemical mutagenesis, the use of specific mutator host strains, recursive ensemble mutagenesis, combinatorial cassette mutagenesis and DNA shuffling as well as a combination of these methods.
  • One or more, preferably, eight to forty, preferably fifteen cycles of molecular directed evolution are preferably be carried out, until DNA-recombining enzymes are evolved that are ac-tive on the intermediate target sites.
  • The molecular directed evolution employed is preferably a substrate-linked protein evolution, in particular as described in WO 2002044409 A2. However other directed evolution strate-gies, such as continuous evolution (WO2012088381 A2) are also applicable.
  • Then preferably a further set of intermediate target sites is created by further adapting the sequences to the half sites of the target sites (selected in a1)), preferably by replacing one or more, preferably up to three nucleotides that differ by the nucleotides that occur in the corresponding half sites of the target site.
  • The sub steps of molecular directed evolution, shuffling and creating further sets of intermediate target sites is preferably performed until the intermediate target sites only differ in one to three, preferably one or two, nucleotides per half site from the half sites of the target sites (selected in a1)).
  • In one preferred embodiment to obtain DNA recombining enzymes that induce a replacement (DRiGR) step b) is carried out in three sub-steps of molecular evolution.
  • In the first step the library or libraries are evolved as described before for DRiGD without an artificial sequence (thus without a donor vector) to select for recombinases that show at least some activity on the target site, thus DNA recombining enzymes that show at least excision activity. This first step is preferably performed as described before firstly on a first set of intermediate target sites and then optionally on a second set of intermediate target sites and finally on the final target sites.
  • In a second step the library or libraries of DNA recombining enzymes obtained as result of step i) are evolved using an artificial sequence with a positive selection marker, like an antibiotic resistance gene. The artificial sequence is preferably a replication deficient donor vector. Thus, a plasmid or linear sequence that does not carry an origin of replication that is active in the host cells is used (as exemplified in FIGS. 9A-9C). The second step is preferably performed on the final target sites. In the second step DNA recombining enzymes are selected that are capable to replace a sequence by DRiGR (which is a more complex reaction than a pure excision). Preferably 5 to 20 cycles, more preferably 8 to 15 cycles, of substrate linked molecular evolution are performed until enzymes are obtained that are active on the final target sites. The use of a replication deficient donor vector in combination with an antibiotic selection marker makes it possible to separate the first recombination reaction (integration) on the first target site, from the second recombination reaction (excision) on the second target site, necessary for a successful DRiGR reaction.
  • In a third step, molecular evolution is performed with the library or libraries of DNA recombining enzymes obtained as result of step ii) with an artificial sequence without a positive selection marker. The artificial sequence is preferably a donor vector that carries a functional origin of replication that is active in the host cells used (as exemplified in FIG. 14 ). The third step is preferably performed on the final target sites. Preferably 5 to 20 cycles, more preferably 8 to 15 cycles, of substrate linked molecular evolution are performed until enzymes are obtained that are active on the final target sites. In this third step, DNA recombining enzymes are selected that show a high replacement activity without the need of a positive selection marker and with similar recombination kinetics on target site 1 and target site 2. This is an important point as for many uses (in particular a medical use or also when generating multi cellular organism with a modified sequence) the presence of a positive selection marker (e. g. antibiotic resistance gene) is not a favorable option.
  • This three-step method has been shown to be very efficient to select for enzymes that induce a replacement (DRiGR) in particular when combined with a vector that comprises one or several recognition sites for the same or in particular for two to three (or even up to five) dif-ferent restriction enzymes between the target sites.
  • In general, and throughout this specification, the term “vector” refers to a nucleic acid molecule capable of transporting a nucleic acid to which it has been linked. Vectors include, but are not limited to, nucleic acid molecules that are single-stranded, double-stranded, or partially double-stranded; nucleic acid molecules that comprise one or more free ends, no free ends (e.g. circular); nucleic acid molecules that comprise DNA, RNA, or both; and other varieties of polynucleotides known in the art. One type of vector is a “plasmid,” which refers to a circular double stranded DNA loop into which additional DNA segments can be inserted, such as by standard molecular cloning techniques. Another type of vector is a viral vector, wherein virally-derived DNA or RNA sequences are present in the vector for packaging into a virus (e.g. retroviruses, replication defective retroviruses, adenoviruses, replication defective adenoviruses, and adeno-associated viruses—AAV). Viral vectors also include polynucleotides carried by a virus for transfection into a host cell. Certain vectors are capable of autonomous replication in a host cell into which they are introduced (e.g. bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors (e.g., non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome.
  • Moreover, certain vectors are capable of directing the expression of genes to which they are operatively-linked. Such vectors are referred to herein as “expression vectors.” Common expression vectors of utility in recombinant DNA techniques are often in the form of plasmids.
  • The expression vector is preferably a plasmid, a virus or an artificial chromosome.
  • Another object of the invention are the vectors or vector as used in the method.
  • Preferred expression vectors comprise:
      • i) One, two, three or four genes each encoding a (different) library of DNA-recombining enzymes, whereas the genes encoding the libraries are under the control of the same promoter,
      • ii) a substrate nucleic acid comprising the first target site and the second target site that surround a negative selection marker, which is preferably a recognition site of a restriction enzyme, that does only occur once in the expression vector (an unique recognition site).
  • To select for DNA recombining enzymes that induce a deletion (DRiGD) of the site of interest, no further substrate DNA is needed, as the recombination will solely take place between the intermediate first and intermediate second target site on the expression vector.
  • To select for DNA recombining enzymes that induce a replacement (DRiGR) of the site of interest by a desired sequence preferably a synthetic nucleic acid (preferably a replication deficient plasmid) comprising the intermediate first target site and/or the intermediate second target site is used as an additional (donor) vector. Preferably, a positive selection marker lies in between the first and second intermediate target site. Thus, the first and second intermediate target site preferably surround a positive selection marker, like an antibiotic resistance gene).
  • The method according to the invention for preparing DNA-recombining enzymes may comprise the further step of:
      • c) isolating the nucleic acid of the least one DNA-recombining enzyme obtained in step b) from the library; and preferably
      • d) cloning the nucleic acid obtained in step c) into a suitable delivery vector.
  • The invention comprises also the designer DNA-recombining enzymes obtained by the method according to the invention.
  • With the presented invention the inventors provide a solution for recombination-based genome editing that allows flexible and flawless alteration of genetic information. The methods allow in particular for the generation of DNA-recombining enzymes to replace genetic mutations causing human diseases, or to delete predefined genetic material from cells. Thus, the DNA-recombining enzymes according to the invention are used to alter a nucleotide sequence, in particular to repair a mutation or to delete a sequence from the genome or a sequence introduced by a virus. The mutations that can be repaired according to the invention in particular comprise point mutations, frame shift mutations, deletions and insertions.
  • Advantageously the DNA recombining enzyme can use target sequences naturally occurring in a genome, including the genome of a host cell, a host organ or a host organism or even the genome of a pathogen, like a virus. An advantage of the invention is that it allows precise site directed altering of DNA without engaging host DNA repair pathways and thereby works without inducing random insertions and deletions (indels).
  • Thus, the invention comprises also a method to modify the genome by inducing site-specific recombination on naturally occurring target sites that are not identical. The method comprises delivery of a DNA recombining enzyme according to the invention into the host, by either delivery of the protein itself, or introducing and expression of a nucleotide sequence encoding it, either from an mRNA or from a DNA template.
  • As defined above “naturally occurring” means that the target sites are not artificially introduced—there are natively present in the same genome.
  • Suitable host cells are in particular eukaryotic cells, including stem cells like hematopoietic stem cells, neuronal stem cells, adipose tissue derived stem cells, fetal stem cells, umbilical cord stem cells, induced pluripotent stem cells and embryonic stem cells. In case of human embryonic stem there are preferably not derived from the destruction of embryos. Further the modification of the human germline and human gametes as host cells are preferably excluded.
  • Suitable host organs for delivery of a DNA recombining enzyme according to the invention comprise any cell in the body, including bone marrow, the skin, muscles, the liver, the lungs, lymph node and the spleen.
  • The method according to the invention can be performed in vitro (outside of a living organism) or in vivo. In vitro includes the manipulation of isolated cells and/or organs.
  • In one aspect, the invention provides a method of replacing or deleting a sequence of interest in a genome as described above.
  • In one aspect the invention relates to the (preferably non-therapeutic) use of a DNA recombining enzyme according to the invention to create an cell or a (preferably non human) multicellular organism with a knock-out of sequence of interest (e. g. a gene or exon) or carrying a modified sequence (e.g. a transgene).
  • In the method the DNA recombining enzyme according to the invention or the genes encoding the enzyme according to the invention (one gene per monomer) are introduced into a cell or organism by a delivery method.
  • The designer DNA-recombining enzyme according to the invention induces a site-specific recombination in a genome on two non-identical target sites that surround a sequence of interest.
  • To modify the genome by inducing site-specific recombination for deletion (DRiGD) of a sequence of interest (e. g. an insertion or a viral sequence) no additional DNA or RNA is necessary.
  • To modify the genome by inducing site-specific recombination for replacement (DRiGR) of a sequence of interest (e.g. a point mutation) the DNA recombining enzyme according to the invention a synthetic sequence comprising a desired sequence (Donor DNA) is additionally introduced into the host. The synthetic sequence comprises the desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site that are recognized by the DNA recombining enzyme according to the invention.
  • In another aspect, the invention provides a method of generating a model eukaryotic cell comprising a mutated or altered disease associated-gene or polynucleotide or a mutated or altered sequences, selected from signaling biochemical pathway-associated genes and polynucleotides. Examples of these genes and polynucleotides are listed below in table A to C.
  • Another application of the invention is to specifically alter a sequence of interest in a genome, in particular by introducing a mutation or introducing or replacing a nucleotide sequence, e. g. to create a model eukaryotic cell or an animal model, which has preferably a particular mutation that corresponds to a human disease and/or which bears a humanized sequence. Advantageously the method allows the targeted generation of animal models without inducing random insertions and deletions (indels) or other unwanted uncontrolled modification of genetic material. In some embodiments, the introduced mutation is associated with an increase in the risk of having or developing a disease.
  • In a preferred embodiment the method according to the invention is used to create animal models, which are useful for biomedical research, e. g. as models for human diseases. In case the invention is carried out in an animal it is preferably carried out for non-therapeutic use of the animal.
  • Suitable host organisms include invertebrates and vertebrates, particularly Bovidae, Drosophila melanogaster, Caenorhabditis elegans, Xenopus laevis, medaka, zebrafish, Mus musculus, Ratus norvegicus or embryos of these organisms.
  • However, also plants and fungi as well as their cells can be used as hosts.
  • In some embodiments, one or more vectors described herein are used to produce a non-human transgenic animal or transgenic plant. In some embodiments, the transgenic animal is a mammal, such as a mouse, rat, or rabbit. In certain embodiments, the organism or subject is a plant. In certain embodiments, the organism or subject or plant is algae. Methods for producing transgenic plants and animals are known in the art, and generally begin with a method of cell transfection. Transgenic animals are also provided, as are transgenic plants, especially crops and algae. The transgenic animal or plant may be useful in applications outside of providing a disease model. These may include food or feed production through expression of, for instance, higher protein, carbohydrate, nutrient or vitamins levels than would normally be seen in the wildtype. In this regard, transgenic plants, especially pulses and tubers, and animals, especially mammals such as livestock (cows, sheep, goats and pigs), but also poultry and edible insects, are preferred.
  • For introducing and expression of the DNA recombining enzyme into a host a delivery vector can be used or other delivery methods, such as DNA, mRNA or protein packaged in liposomes or nanoparticles (e.g. as described by Wang M et al. (2016)), or electroporation ex vivo, or cell squeeze are also applicable.
  • The delivery method used depends on the host, host cell or target tissue in the host wherein the sequence of interest shall be altered.
  • Also, the choice of a delivery vector used depends on the host, host cell or target tissue in the host wherein the sequence of interest shall be altered. Suitable delivery vector are known by the person skilled in the art and include plasmids, artificial chromosomes, retroviral vectors, lentiviral vectors, spumavirus vectors and adenoviral vectors but also agrobacteria harboring shuttle vectors. Advantageous delivery vectors include lentiviruses and adeno-associated viruses, and types of such vectors (in particular adeno associated viral vectors) can also be selected for targeting particular types of cells or tissue
  • The delivery vector preferably comprises a regulatory element. The term “regulatory element” is intended to include promoters, enhancers, internal ribosomal entry sites (IRES), and other expression control elements (e.g. transcription termination signals, such as polyadenylation signals and poly-U sequences). In some embodiments the regulatory element is a cellular, bacterial, a viral or a hybrid promoter, wherein said promoter is preferably a constitutive or inducible promoter. Also the promoter is preferably selected depending on the host, host cell or target tissue in the host. Suitable promotors are also known by the person skilled in the art. Preferred constitutive promoters are selected from the promoters of cytomegalovirus, Rous sarcoma virus, murine leukemia virus-related retroviruses, phosphoglycerokinase gene, murine spleen focus-forming virus or human elongation factor 1 alpha.
  • For replacement of a sequence of interest (DRiGR) the delivery vector preferably contains also the donor sequence that comprises the two non-identical target sites recognized by the designer DNA-recombining enzyme according to the invention and whereas the target sites surround a desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site.
  • Alternatively, the donor sequence is provided on a separate nucleic acid molecule or vector (e. g. a replication deficient plasmid)
  • The invention comprises also nucleic acids or vectors encoding for a designer DNA-recombining enzyme according to the invention. In a preferred embodiment the nucleic acids encoding for the monomers of a designer DNA-recombining enzyme according to the invention are one nucleic acid molecule or in one vector. Preferably the monomers of the designer DNA-recombining enzymes are expressed under control of one promotor or other regulatory element.
  • The invention also comprises a method for transduction of a cell with
      • 1. an expression vector or delivery vector as described above encoding a designer DNA-recombining enzyme according to the invention, whereas the designer DNA-recombining enzyme according to the invention induces a site specific recombination in a genome on two non-identical target sites that surround a sequence of interest
      • 2. a nucleotide sequence as donor sequence that comprises the same two non-identical target sites that surround a desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site, and expression of the DNA-recombining enzyme.
  • The donor sequence can be located on the expression or delivery vector or is provided as separate nucleic acid, preferably a replication deficient plasmid. Preferably, the donor sequence is provided at multiple copies per cell to increase the likelihood of replacement of the target sequence (see also FIG. 3 ).
  • In a further aspect the invention relates a set or kit comprising:
      • i) a designer DNA-recombining enzyme according to the invention or a nucleic acid or vector encoding for a designer DNA-recombining enzyme according to the invention, whereas the designer DNA-recombining enzyme according to the invention induces a site specific recombination in a genome on two non-identical target sites that surround a sequence of interest;
      • ii) a nucleotide sequence as donor sequence that comprises the same two non-identical target sites that surround a desired sequence (Donor DNA) that replaces the sequence of interest, whereas desired sequence is flanked by the first target site and the second target site. Preferably, the donor sequence is provided at multiple copies per cell to increase the likelihood of replacement of the target sequence (see also FIG. 3 ).
  • Again, the donor sequence is either located on the same vector (encoding for the designer DNA-recombining enzyme according to the invention) or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • Generally, for a replacement according to the invention (DRiGR) the desired sequence on the donor sequence is preferably a sequence that corresponds to the sequence of interest, but wherein the mutation is replaced by the wild type sequence (a gene or part thereof like an exon) or a functional variant thereof (e. g. a silent mutation or a mutation that increase the activity).
  • A further important part of the invention is the medical application of the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention.
  • For a medical application the sequence of interest is a “disease-associated” gene or polynuclotide, that is altered to cure the disease, or at least reduce the symptoms or to prolong life.
  • In one aspect the invention relates to an in vitro method. In this method the invention is applied to isolated cells or organs as described above.
  • In another aspect the invention relates to a method of the treatment of subject, preferably a human or animal, comprising the step of applying a DNA recombining enzyme according to the invention, a nucleic acid or vector encoding for a designer DNA-recombining enzyme according to the invention or the set according to the invention.
  • Delivery methods and vectors are preferably selected as described above.
  • In one embodiment the subject to be treated carries a mutation in a nucleotide sequence (sequence of interest) that causes the subject to suffer from a disease, in particular a mutation that causes a reduced function or loss of function of the gene-product, e. g. a mutation in the factor 9 gene that causes hemophilia. By delivery of the DNA recombining enzyme according to the invention, a nucleic acid or vector encoding it and a donor sequence carrying a desired sequence, a wild type or other functional variant of the gene (or a part thereof—like an exon) the mutant part of the gene is replaced by the desired (functional) sequence. To enable site-specific recombination the desired sequences in the donor sequence is flanked by the target sites for the DNA recombining enzyme.
  • In another embodiment the subject to be treated carries a nucleotide sequence (sequence of interest) that causes a disease or the inhibition reduces the symptoms thereof. Here the sequence of interest is deleted by applying the method according to the invention and delivery of the DNA recombining enzyme according to the invention, a nucleic acid or vector encoding it. Here, the sequence of interest is in particular a sequence of a pathogen (a microorganism like a bacteria, a virus or a parasite) or an oncogene or another gene, whereas the activity causes a disease or the inhibition of gene activity is known to reduce symptoms (like for example the gene encoding PCSK9).
  • Further the invention comprises a pharmaceutical composition comprising a DNA recombining enzyme according to the invention, a nucleic acid or vector encoding for a designer DNA-recombining enzyme according to the invention or the set according to the invention as well as suitable carriers.
  • The sequence of interested targeted by a DNA recombining enzyme according to the invention can be any polynucleotide endogenous or exogenous to the eukaryotic cell. For example, the sequence of interested can be a polynucleotide residing in the nucleus or mitochondria of the eukaryotic cell or a virus integrated into the genome of the host cell or not. The sequence of interested can be a sequence coding a gene product (e.g., a protein) or a noncoding sequence (e.g., an intron or a regulatory polynucleotide).
  • The sequence of interested targeted by a DNA recombining enzymes according to the invention may include a number of disease-associated genes and polynucleotides as well as signaling biochemical pathway-associated genes and polynucleotides.
  • A “disease-associated” gene or polynucleotide refers to any gene or polynucleotide which is yielding transcription or translation products at an abnormal level or in an abnormal form in cells derived from a disease-affected tissues compared with tissues or cells of a non disease control. It may be a gene that is expressed at an abnormally high level; it may be a gene that becomes expressed at an abnormally low level, where the altered expression correlates with the occurrence and/or progression of the disease. A disease-associated gene also refers to a gene possessing mutation(s) or genetic variation that is directly responsible or is in linkage disequilibrium with a gene(s) that is responsible for the etiology of a disease. The transcribed or translated products may be known or unknown and may be at a normal or abnormal level. Examples of disease-associated genes and polynucleotides are available from McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, Md.) and National Center for Biotechnology Information, National Library of Medicine (Bethesda, Md.), available on the World Wide Web.
  • The sequence of interested targeted by a DNA recombining enzymes according to the invention may include a number of disease-associated genes and polynucleotides as well as signaling biochemical pathway-associated genes and polynucleotides as listed in the US patent U.S. Pat. No. 8,697,359 B1, the contents of which is herein incorporated by reference in its entirety.
  • Examples of disease-associated genes and polynucleotides are listed in Tables A and B. Disease specific information is available from McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, Md.) and National Center for Biotechnology Information, National Library of Medicine (Bethesda, Md.), available on the World Wide Web. Examples of signaling biochemical pathway-associated genes and polynucleotides are listed in Table C.
  • Mutations in these genes and pathways can result in production of improper proteins or proteins in improper amounts which affect function. Further examples of genes, diseases and proteins are hereby incorporated by reference from U.S. Provisional application. Such genes, proteins and pathways may be the sequence of interest targeted by a DNA recombining enzymes according to the invention.
  • TABLE A
    DISEASE/
    DISORDER GENE(S)
    Neoplasia PTEN; ATM; ATR; EGFR; ERBB2; ERBB3; ERBB4; Notch1; Notch2; Notch3;
    Notch4; AKT; AKT2; AKT3; HIF; HIF1a; HIF3a; Met; HRG; Bcl2;
    PPAR alpha; PPAR gamma; WT1 (Wilms Tumor); FGF Receptor Family
    members (5 members: 1, 2, 3, 4, 5); CDKN2a; APC; RB (retinoblastoma); MEN1;
    VHL; BRCA1; BRCA2; AR (Androgen Receptor); TSG101; IGF; IGF Receptor;
    Igf1 (4 variants); Igf2 (3 variants); Igf 1 Receptor; Igf 2 Receptor; Bax; Bcl2;
    caspases family (9 members: 1, 2, 3, 4, 6, 7, 8, 9, 12); Kras; Apc
    Age-related Macular Aber; Ccl2; Cc2; cp (ceruloplasmin); Timp3; cathepsinD; Vldlr; Ccr2
    Degeneration
    Schizophrenia Neuregulin1 (Nrg1); Erb4 (receptor for Neuregulin); Complexin1 (Cplx1); Tph1
    Tryptophan hydroxylase; Tph2 Tryptophan hydroxylase 2; Neurexin 1; GSK3;
    GSK3a; GSK3b
    Disorders 5-HTT (Slc6a4); COMT; DRD (Drd1a); SLC6A3; DAOA; DTNBP1; Dao (Dao1)
    Trinucleotide HTT (Huntington's Dx); SBMA/SMAX1/AR (Kennedy's Dx); FXN/X25
    Repeat Disorders (Friedrich's Ataxia); ATX3 (Machado-Joseph's Dx); ATXN1 and ATXN2
    (spinocerebellar ataxias); DMPK (myotonic dystrophy); Atrophin-1 and Atn 1
    (DRPLA Dx); CBP (Creb-BP-global instability); VLDLR (Alzheimer's); Atxn7; Atxn10
    Fragile X Syndrome FMR2; FXR1; FXR2; mGLUR5
    Secretase Related. APH-1 (alpha and beta); Presenilin (Psen1); nicastrin (Ncstn); PEN-2
    Disorders
    Other Prion-related Nos1; Parp1; Nat1; Nat2, Prp
    disorders
    ALS SOD1; ALS2; STEX; FUS; TARDBP; VEGF (VEGF-a; VEGF-b; V EGF-c)
    Drug addiction Prkce (alcohol); Drd2; Drd4; ABAT (alcohol); GRIA2; Grm5; Grin1; Htr1b;
    Grin2a; Drd3; Pdyn; Gria1 (alcohol)
    Autism Mecp2; BZRAP1; MDGA2; Sema5A; Neurexin 1; Fragile X (FMR2 (AFF2);
    FXR1; FXR2; Mglur5)
    Alzheimer's E1; CHIP; UCH; UBB; Tau; LRP; PICALM; Clusterin; PS1; SORL1; CR1; Vld1r;
    Disease Uba1; Uba3; CHIP28 (Aqp1, Aquaporin 1); Uchl1; Uchl3; APP
    Inflammation 1L-10; IL-1 (1L-1a; IL-1b); 1L-13; IL-17 (IL-17a, CTLA8); IL-17b; IL-17c; IL-17d;
    IL-17f); II-23; Cx3er1; ptpn22; TNFa; NOD2/CARD15 for IBD; IL-6; 1L-12
    (1L-12a; 1L-12b); CTLA4; Cx3cl1
    Parkinson's x-Synuclein; DJ-1; LRRK2; Parkin; PINK1
    Disease
  • TABLE B
    DISEASE/
    DISORDER GENE(S)
    Cell dysregulation B-cell non-Hodgkin lymphoma (BCL7A, BCL7); Leukemia
    and oncology (TAL1 TCL5, SCL, TAL2, FLT3, NBS1, NBS, ZNFN1A1, IK1,
    diseases and LYF1, HOXD4, OX4B, BCR, CML, PHL, ALL, ARNT, KRAS2, RASK2,
    disorders GMPS, AF10, ARH-GEF12, LARG, KIAA0382, CALM, CLTH, CEBPA,
    CEBP, CHIC2, BTL, FLT3, KIT, PBT, LPP, NPM1, NUP214, D9S46E,
    CAN, CAIN, RUNX1, CBFA2, AML1, WHSC1L1, NSD3, FLT3, AF1Q, NPM1,
    NUMA1, NF145, PLZF, PML, MYL, STAT5B, AF10, CALM, CLTH, ARL11,
    ARLTS1, P2RX7, P2X7, BCR,CML, PHL, ALL, GRAF, NF1, VRNF, WSS,
    NFNS, PTPN11, PTP2C, SHP2, NS1, BCL2, CCND1, PRAD1, BCL1, TCRA,
    GATA1, GF1, ERYF1, NFE1, ABL1, NQO1, DIA4, NMOR1, NUP214, D9S46E,
    CAN, CAIN).
    Inflammation and AIDS (KIR3DL1, NKAT3, NKB1, AMB11, KIR3DS1, IFNG, CXCL12, SDF1);
    immune related Autoimmune lymphoproliferative syndrome (TNFRSF6, APT1, FAS, CD95,
    diseases and ALPS1A); Combined immuno-deficiency, (IL2RG, SCIDX1, SCIDX, IMD4);
    disorders HIV-1 (CCL5, SCYA5, D17S136E, TCP228), HIV susceptibility or infection
    (IL10, CSIF, CMKBR2, CCR2, CMKBR5, CCCKR5 (CCR5)); Immuno-deficiencies
    (CD3E, CD3G, AICDA, AID, HIGM2, TNFRSF5, CD40, UNG, DGU, HIGM4, TNFSF5,
    CD40LG, HIGM1, IGM, FOXP3, IPEX, AIID, XPID, PIDX, TNFRSF14B, TACI);
    Inflammation (IL-10, IL-1 (IL-1 a, IL-1b), IL-13, IL-17 (IL-17a (CTLA8)),
    IL-17b, IL-17c, IL-17d, IL-17f, ll-23, Cx3cr1, ptpn22, TNFa, OD2/CARD15
    for IBD, IL-6, IL-12 (IL-12a, IL-12b), CTLA4, Cx3cl1); Severe combined
    immunodeficiencies (SCIDs) (JAK3, JAKL, DCLRE1C,
    ARTEMIS, SCIDA, RAG1, RAG2, ADA, PTPRC, CD45, LCA, IL7R, CD3D,
    T3D, IL2RG, SCIDX1, SCIDX, IMD4).
    Metabolic, liver, Amyloid neuropathy (TTR, PALB); Amyloidosis (APOA1, APP, AAA, CVAP, AD1,
    kidney and protein GSN, FGA, LYZ, TTR, PALB); Cirrhosis (KRT18, KRT8, CIRH1A, NAIC, TEX292,
    diseases and KIAA1988); Cystic fibrosis (CFTR, ABCC7, CF, MRP7); Glycogen storage diseases
    disorders (SLC2A2, GLUT2, G6PC, G6PT, G6PT1, GAA, LAMP2, LAMPB, AGL, GDE, GBE1, GYS2,
    PYGL, PFKM); Hepatic adenoma, 142330 (TCF1, HNF1A, MODY3), Hepatic failure,
    early onset, and neurologic disorder (SCOD1, SCO1), Hepatic lipase deficiency
    (LIPC), Hepatoblastoma, cancer and carcinomas (CTNNB1, PDGFRL, PDGRL, PRLTS,
    AXIN1, AXIN, CTNNB1, TP53, P53, LFS1, IGF2R, MPRI, MET, CASP8, MCH5; Medullary
    cystic kidney disease (UMOD, HNFJ, FJHN, MCKD2, ADMCKD2); Phenylketonuria
    (PAH, PKU1, QDPR, DHPR, PTS); Polycystic kidney and hepatic disease (FCYT,
    PKHD1, ARPKD, PKD1, PKD2, PKD4, PKDTS, PRKCSH, G19P1, PCLD, SEC63).
    Muscular/Skeletal Becker muscular dystrophy (DMD, BMD, MYF6), Duchenne Muscular Dystrophy
    diseases and (DMD, BMD); Emery-Dreifuss muscular dystrophy (LMNA, LMN1, EMD2, FPLD,
    disorders CMD1A, HGPS, LGMD1B, LMNA, LMN1, EMD2, FPLD, CMD1A); Facio-scapulohumeral
    muscular dystrophy (FSHMD1A, FSHD1A); Muscular dystrophy (FKRP, MDC1C, LGMD2I,
    LAMA2, LAMM, LARGE, KIAA0609, MDC1D, FCMD, TTID, MYOT, CAPN3, CANP3, DYSF,
    LGMD2B, SGCG, LGMD2C, DMDA1, SCG3, SGCA, ADL, DAG2, LGMD2D, DMDA2, SGCB,
    LGMD2E, SGCD, SGD, LGMD2F, CMD1L, TCAP, LGMD2G, CMD1N, TRIM32, HT2A, LGMD2H,
    FKRP, MDC1C, LGMD2I, TTN, CMD1G, TMD, LGMD2J, POMT1, CAV3, LGMD1C, SEPN1,
    SELN, RSMD1, PLEC1, PLTN, EBS1); Osteopetrosis (LRP5, BMND1, LRP7, LR3,
    OPPG, VBCH2, CLCN7, CLC7, OPTA2, OSTM1, GL, TCIRG1, TIRC7, OC116, OPTB1);
    Muscular atrophy (VAPB, VAPC, ALS8, SMN1, SMA1, SMA2, SMA3, SMA4, BSCL2,
    SPG17, GARS, SMAD1, CMT2D, HEXB, IGHMBP2, SMUBP2, CATF1, SMARD1).
    Neurological and ALS (SOD1, ALS2, STEX, FUS, TARDBP, VEGF (VEGF-a, VEGF-b, VEGF-c);
    neuronal diseases Alzheimer disease (APP, AAA, CVAP, AD1, APOE, AD2, PSEN2, AD4, STM2,
    and disorders APBB2, FE65L1, NOS3, PLAU, URK, ACE, DCP1, ACE1, MPO, PACIP1, PAXIP1L,
    PTIP, A2M, BLMH, BMH, PSEN1, AD3); Autism (Mecp2, BZRAP1, MDGA2, Sema5A,
    Neurexin 1, GLO1, MECP2, RTT, PPMX, MRX16, MRX79, NLGN3, NLGN4, KIAA1260,
    AUTSX2); Fragile X Syndrome (FMR2, FXR1, FXR2, mGLUR5); Huntington's
    disease and disease like disorders (HD, IT15, PRNP, PRIP, JPH3, JP3, HDL2,
    TBP, SCA17); Parkinson disease (NR4A2, NURR1, NOT, TINUR, SNCAIP, TBP, SCA17,
    SNCA, NACP, PARK1, PARK4, DJ1, PARK7, LRRK2, PARK8, PINK1, PARK6, UCHL1, PARK5,
    SNCA, NACP, PARK1, PARK4, PRKN, PARK2, PDJ, DBH, NDUFV2); Rett syndrome (MECP2,
    RTT, PPMX, MRX16, MRX79, CDKL5, STK9, MECP2, RTT, PPMX, MRX16, MRX79, x-Synuclein,
    DJ-1); Schizophrenia (Neuregulin1 (Nrg1), Erb4 (receptor for Neuregulin), Complexin1
    (Cplx1), Tph1 Tryptophan hydroxylase, Tph2, Tryptophan hydroxylase 2, Neurexin 1, GSK3,
    GSK3a, GSK3b, 5-HTT (Slc6a4), COMT, DRD (Drd1a), SLC6A3, DAOA, DTNBP1, Dao (Dao1));
    Secretase Related Disorders (APH-1 (alpha and beta), Presenilin (Psen1), nicastrin,
    (Ncstn), PEN-2, Nos1, Parp1, Nat1, Nat2); Trinucleotide Repeat Disorders (HTT
    (Huntington's Dx), SBMA/SMAX1/AR (Kennedy's Dx), FXN/X25 (Friedrich's Ataxia),
    ATX3 (Machado- Joseph's Dx), ATXN1 and ATXN2 (spinocerebellar ataxias), DMPK
    (myotonic dystrophy), Atrophin-1 and Atn1 (DRPLA Dx), CBP (Creb-BP - global instability),
    VLDLR (Alzheimer's), Atxn7, Atxn10).
    Occular diseases Age-related macular degeneration (Aber, Ccl2, Cc2, cp (ceruloplasmin), Timp3, cathepsinD,
    and disorders Vldlr, Ccr2); Cataract (CRYAA, CRYA1, CRYBB2, CRYB2, PITX3, BFSP2, CP49, CP47, CRYAA,
    CRYA1, PAX6, AN2, MGDA, CRYBA1, CRYB1, CRYGC, CRYG3, CCL, LIM2, MP19, CRYGD, CRYG4,
    BFSP2, CP49, CP47, HSF4, CTM, HSF4, CTM, MIP, AQP0, CRYAB, CRYA2, CTPP2, CRYBB1, CRYGD,
    CRYG4, CRYBB2, CRYB2, CRYGC, CRYG3, CCL, CRYAA, CRYA1, GJA8, CX50, CAE1, GJA3, CX46, CZP3,
    CAE3, CCM1, CAM, KRIT1); Corneal clouding and dystrophy (APOA1, TGFBI, CSD2, CDGG1, CSD,
    BIGH3, CDG2, TACSTD2, TROP2, M1S1, VSX1, RINX,, PPCD, PPD, KTCN, COL8A2, FECD, PPCD2,
    PIP5K3, CFD); Cornea plana congenital (KERA, CNA2); Glaucoma (MYOC, TIGR, GLC1A, JOAG, GPOA,
    OPTN, GLC1E, FIP2, HYPL, NRP, CYP1B1, GLC3A, OPA1, NTG, NPG, CYP1B1, GLC3A); Leber
    congenital amaurosis (CRB1, RP12, CRX, CORD2, CRD, RPGRIP1, LCA6, CORD9, RPE65, RP20,
    AIPL1, LCA4, GUCY2D, GUC2D, LCA1, CORD6, RDH12, LCA3); Macular dystrophy (ELOVL4,
    ADMD, STGD2, STGD3, RDS, RP7, PRPH2, PRPH, AVMD, AOFMD, VMD2).
    Epilepsy, myoclonic, EPM2A, MELF, EPM2, NHLRC1, EPM2A, EPM2B
    Lafora type, 254780
    Duchenne muscular DMD, BMD
    dystrophy, 310200
    AIDS, delayed/rapid KIR3DL1, NKAT3, NKB1, AMB11, KIR3DS1
    progression to
    AIDS, rapid progression to, IFNG
    609423
    AIDS, resistance to CXCL12, SDF1
    Alpha 1-Antitrypsin SERPINA1 [serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin),
    Deficiency member 1]; SERPINA2 [serpin peptidase inhibitor, clade A (alpha-1 antiproteinase,
    antitrypsin), member 2]; SERPINA3 [serpin peptidase inhibitor, clade A (alpha-1
    antiproteinase, antitrypsin), member 3]; SERPINA5 [serpin peptidase inhibitor, clade A
    (alpha-1 antiproteinase, antitrypsin), member 5]; SERPINA6 [serpin peptidase inhibitor,
    clade A (alpha-1 antiproteinase, antitrypsin), member 6]; SERPINA7 [serpin peptidase
    inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7];” AND “SERPLNA6
    (serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6)
  • TABLE C
    CELLULAR
    FUNCTION GENES
    PI3K/AKT Signaling PRKCE; ITGAM; ITGA5; IRAK1; PRKAA2; EIF2AK2; PTEN; EIF4E; PRKCZ;
    GRK6; MAPK1; TSC1; PLK1; AKT2; IKBKB; PIK3CA; CDK8; CDKN1B; NFKB2;
    BCL2; PIK3CB; PPP2R1A; MAPK8; BCL2L1; MAPK3; TSC2; ITGA1; KRAS;
    EIF4EBP1; RELA; PRKCD; NOS3; PRKAA1; MAPK9; CDK2; PPP2CA; PIM1;
    ITGB7; YWHAZ; ILK; TP53; RAF1.; IKBKG; RELB; DYRK1A; CDKN1A; ITGB1;
    MAP2K2; JAK1; AKT1; JAK2; PIK3R1; CHUK; PDPK1; PPP2R5C; CTNNB1.; MAP2K1;
    NFKB1; PAK3; ITGB3; CCND1; GSK3A; FRAP1; SFN; ITGA2; TTK; CSNK1A1; BRAF;
    GSK3B; AKT3; FOXO1; SGK; HSP90AA1; RPS6KB1
    ERK/MAPK Signaling PRKCE; ITGAM; ITGA5; HSPB1; IRAK1; PRKAA2; EIF2AK2; RAC1; RAP1A; TLN1;
    EIF4E; ELK1; GRK6; MAPK1; RAC2; PLK1; AKT2; PIK3CA; CDK8; CREB1; PRKCI;
    PTK2; FOS; RPS6KA4; PIK3CB; PPP2R1A; PIK3C3; MAPK8; MAPK3; ITGA1; ETS1;
    KRAS; MYCN; EIF4EBP1; PPARG; PRKCD; PRKAA1; MAPK9; SRC; CDK2; PPP2CA;
    PIM1; PIK3C2A; ITGB7; YWHAZ; PPP1CC; KSR1; PXN; RAF1; FYN; DYRK1A; ITGB1;
    MAP2K2; PAK4; PIK3R1; STAT3; PPP2R5C; MAP2K1; PAK3; ITGB3; ESR1; ITGA2;
    MYC; TTK; CSNK1A1; CRKL; BRAF; ATF4; PRKCA; SRF; STAT1; SGK
    Glucocorticoid Receptor RAC1; TAF4B; EP300; SMAD2; TRAF6; PCAF; ELK1; MAPK1; SMAD3; AKT2;
    Signaling IKBKB; NCOR2; UBE2I; PIK3CA; CREB1; FOS; HSPA5; NFKB2; BCL2; MAP3K14;
    STAT5B; PIK3CB; PIK3C3; MAPK8; BCL2L1; MAPK3; TSC22D3; MAPK10; NRIP1;
    KRAS; MAPK13; RELA; STAT5A; MAPK9; NOS2A; PBX1; NR3C1; PIK3C2A; CDKN1C;
    TRAF2; SERPINE1; NCOA3; MAPK14; TNF; RAF1; IKBKG; MAP3K7; CREBBP; CDKN1A;
    MAP2K2; JAK1; IL8; NCOA2; AKT1; JAK2; PIK3R1; CHUK; STAT3; MAP2K1; NFKB1;
    TGFBR1; ESR1; SMAD4; CEBPB; JUN; AR; AKT3; CCL2; MMP1; STAT1; IL6; HSP90AA1
    Axonal Guidance PRKCE; ITGAM; ROCK1; ITGA5; CXCR4; ADAM12; IGF1; RAC1; RAP1A; E1F4E; PRKCZ;
    Signaling NRP1; NTRK2; ARHGEF7; SMO; ROCK2; MAPK1; PGF; RAC2; PTPN11; GNAS; AKT2;
    PIK3CA; ERBB2; PRKC1; PTK2; CFL1; GNAQ; PIK3CB; CXCL12; PIK3C3; WNT11;
    PRKD1; GNB2L1; ABL1; MAPK3; ITGA1; KRAS; RHOA; PRKCD; PIK3C2A; ITGB7; GLI2;
    PXN; VASP; RAF1; FYN; ITGB1; MAP2K2; PAK4; ADAM17; AKT1; PIK3R1; GLI1; WNT5A;
    ADAM10; MAP2K1; PAK3; ITGB3; CDC42; VEGFA; ITGA2; EPHA8; CRKL; RND1; GSK3B;
    AKT3; PRKCA
    Ephrin Receptor PRKCE; ITGAM; ROCK1; ITGA5; CXCR4; IRAK1; PRKAA2; EIF2AK2; RAC1; RAP1A;
    Signaling GRK6; ROCK2; MAPK1; PGF; RAC2; PTPN11; GNAS; PLK1; AKT2; DOK1; CDK8;
    CREB1; PTK2; CFL1; GNAQ; MAP3K14; CXCL12; MAPK8; GNB2L1; ABL1; MAPK3;
    ITGA1; KRAS; RHOA; PRKCD; PRKAA1; MAPK9; SRC; CDK2; PIM1; ITGB7; PXN;
    RAF1; FYN; DYRK1A; ITGB1; MAP2K2; PAK4, AKT1; JAK2; STAT3; ADAM10;
    MAP2K1; PAK3; ITGB3; CDC42; VEGFA; ITGA2; EPHA8; TTK; CSNK1A1; CRKL;
    BRAF; PTPN13; ATF4; AKT3; SGK
    Actin Cytoskeleton ACTN4; PRKCE; ITGAM; ROCK1; ITGA5; IRAK1; PRKAA2; EIF2AK2; RAC1; INS;
    Signaling ARHGEF7; GRK6; ROCK2; MAPK1; RAC2; PLK1; AKT2; PIK3CA; CDK8; PTK2;
    CFL1; PIK3CB; MYH9; DIAPH1; PIK3C3; MAPK8; F2R; MAPK3; SLC9A1; ITGA1;
    KRAS; RHOA; PRKCD; PRKAA1; MAPK9; CDK2; PIM1; PIK3C2A; ITGB7; PPP1CC;
    PXN; VIL2; RAF1; GSN; DYRK1A; ITGB1; MAP2K2; PAK4; PIP5K1A; PIK3R1;
    MAP2K1; PAK3; ITGB3; CDC42; APC; ITGA2; TTK; CSNK1A1; CRKL; BRAF; VAV3; SGK
    Huntington's Disease PRKCE; IGF1; EP300; RCOR1.; PRKCZ; HDAC4; TGM2; MAPK1; CAPNS1; AKT2;
    Signaling EGFR; NCOR2; SP1; CAPN2; PIK3CA; HDAC5; CREB1; PRKC1; HSPA5; REST;
    GNAQ; PIK3CB; PIK3C3; MAPK8; IGF1R; PRKD1; GNB2L1; BCL2L1; CAPN1; MAPK3;
    CASP8; HDAC2; HDAC7A; PRKCD; HDAC11; MAPK9; HDAC9; PIK3C2A; HDAC3; TP53;
    CASP9; CREBBP; AKT1; PIK3R1; PDPK1; CASP1; APAF1; FRAP1; CASP2; JUN; BAX;
    ATF4; AKT3; PRKCA; CLTC; SGK; HDAC6; CASP3
    Apoptosis Signaling PRKCE; ROCK1; BID; IRAK1; PRKAA2; EIF2AK2; BAK1; BIRC4; GRK6; MAPK1;
    CAPNS1; PLK1; AKT2; IKBKB; CAPN2; CDK8; FAS; NFKB2; BCL2; MAP3K14;
    MAPK8; BCL2L1; CAPN1; MAPK3; CASP8; KRAS; RELA; PRKCD; PRKAA1; MAPK9;
    CDK2; PIM1; TP53; TNF; RAF1; IKBKG; RELB; CASP9; DYRK1A; MAP2K2; CHUK;
    APAF1; MAP2K1; NFKB1; PAK3; LMNA; CASP2; BIRC2; TTK; CSNK1A1; BRAF;
    BAX; PRKCA; SGK; CASP3; BIRC3; PARP1
    B Cell Receptor RAC1; PTEN; LYN; ELK1; MAPK1; RAC2; PTPN11; AKT2; IKBKB; PIK3CA; CREB1;
    Signaling SYK; NFKB2; CAMK2A; MAP3K14; PIK3CB; PIK3C3; MAPK8; BCL2L1; ABL1; MAPK3;
    ETS1; KRAS; MAPK13; RELA; PTPN6; MAPK9; EGR1; PIK3C2A; BTK; MAPK14; RAF1;
    IKBKG; RELB; MAP3K7; MAP2K2; AKT1; PIK3R1; CHUK; MAP2K1; NFKB1; CDC42; GSK3A;
    FRAP1; BCL6; BCL10; JUN; GSK3B; ATF4; AKT3; VAV3; RPS6KB1
    Leukocyte Extravasation ACTN4; CD44; PRKCE; ITGAM; ROCK1; CXCR4; CYBA; RAC1; RAP1A; PRKCZ; ROCK2;
    Signaling RAC2; PTPN11; MMP14; PIK3CA; PRKCI; PTK2; PIK3CB; CXCL12; PIK3C3; MAPK8;
    PRKD1; ABL1; MAPK10; CYBB; MAPK13; RHOA; PRKCD; MAPK9; SRC; PIK3C2A; BTK;
    MAPK14; NOX1; PXN; VIL2; VASP; ITGB1; MAP2K2; CTNND1; PIK3R1; CTNNB1; CLDN1;
    CDC42; F11R; ITK; CRKL; VAV3; CTTN; PRKCA; MMP1; MMP9
    Integrin Signaling ACTN4; ITGAM; ROCK1; ITGA5; RAC1; PTEN; RAP1A; TLN1; ARHGEF7; MAPK1; RAC2;
    CAPNS1; AKT2; CAPN2; P1K3CA; PTK2; PIK3CB; PIK3C3; MAPK8; CAV1; CAPN1;
    ABL1; MAPK3; ITGA1; KRAS; RHOA; SRC; PIK3C2A; ITGB7; PPP1CC; ILK; PXN;
    VASP; RAF1; FYN; ITGB1; MAP2K2; PAK4; AKT1; PIK3R1; TNK2; MAP2K1; PAK3;
    ITGB3; CDC42; RND3; ITGA2; CRKL; BRAF; GSK3B; AKT3
    Acute Phase Response IRAK1; SOD2; MYD88; TRAF6; ELK1; MAPK1; PTPN11; AKT2; IKBKB; PIK3CA;
    Signaling FOS; NFKB2; MAP3K14; PIK3CB; MAPK8; RIPK1; MAPK3; IL6ST; KRAS; MAPK13;
    IL6R; RELA; SOCS1; MAPK9; FTL; NR3C1; TRAF2; SERPINE1; MAPK14; TNF; RAF1;
    PDK1; IKBKG; RELB; MAP3K7; MAP2K2; AKT1; JAK2; PIK3R1; CHUK; STAT3; MAP2K1;
    NFKB1; FRAP1; CEBPB; JUN; AKT3; IL1R1; IL6
    PTEN Signaling ITGAM; ITGA5; RAC1; PTEN; PRKCZ; BCL2L11; MAPK1; RAC2; AKT2; EGFR; IKBKB;
    CBL; PIK3CA; CDKN1B; PTK2; NFKB2; BCL2; PIK3CB; BCL2L1; MAPK3; ITGA1;
    KRAS; ITGB7; ILK; PDGFRB; INSR; RAF1; IKBKG; CASP9; CDKN1A; ITGB1;
    MAP2K2; AKT1; PIK3R1; CHUK; PDGFRA; PDPK1; MAP2K1; NFKB1; ITGB3; CDC42;
    CCND1; GSK3A; ITGA2; GSK3B; AKT3; FOXO1; CASP3; RPS6KB1
    p53 Signaling PTEN; EP300; BBC3; PCAF; FASN; BRCA1; GADD45A; BIRC5; AKT2; PIK3CA; CHEK1;
    TP53INP1; BCL2; PIK3CB; PIK3C3; MAPK8; THBS1; ATR; BCL2L1; E2F1; PMAIP1;
    CHEK2; TNFRSF10B; TP73; RB1; HDAC9; CDK2; PIK3C2A; MAPK14; TP53; LRDD; CDKN1A;
    HIPK2; AKT1; RIK3R1; RRM2B; APAF1; CTNNB1; SIRT1; CCND1; PRKDC; ATM; SFN;
    CDKN2A; JUN; SNAI2; GSK3B; BAX; AKT3
    Aryl Hydrocarbon HSPB1; EP300; FASN; TGM2; RXRA; MAPK1; NQO1; NCOR2; SP1; ARNT; CDKN1B;
    Receptor FOS; CHEK1; SMARCA4; NFKB2; MAPK8; ALDH1A1; ATR; E2F1; MAPK3; NRIP1; CHEK2;
    Signaling RELA; TP73; GSTP1; RB1; SRC; CDK2; AHR; NFE2L2; NCOA3; TP53; TNF; CDKN1A;
    NCOA2; APAF1; NFKB1; CCND1; ATM; ESR1; CDKN2A; MYC; JUN; ESR2; BAX; IL6;
    CYP1B1; HSP90AA1
    Xenobiotic Metabolism PRKCE; EP300; PRKCZ; RXRA; MAPK1; NQO1; NCOR2; PIK3CA; ARNT; PRKCI; NFKB2;
    Signaling CAMK2A; PIK3CB; PPP2R1A; PIK3C3; MAPK8; PRKD1; ALDH1A1; MAPK3; NRIP1; KRAS;
    MAPK13; PRKCD; GSTP1; MAPK9; NOS2A; ABCB1; AHR; PPP2CA; FTL; NFE2L2; PIK3C2A;
    PPARGC1A; MAPK14; TNF; RAF1; CREBBP; MAP2K2; PIK3R1; PPP2R5C; MAP2K1; NFKB1; KEAP1;
    PRKCA; EIF2AK3; IL6; CYP1B1; HSP90AA1
    SAPK/JNK Signaling PRKCE; IRAK1; PRKAA2; EIF2AK2; RAC1; ELK1; GRK6; MAPK1; GADD45A; RAC2; PLK1;
    AKT2; PIK3CA; FADD; CDK8; PIK3CB; PIK3C3; MAPK8; RIPK1; GNB2L1; IRS1; MAPK3;
    MAPK10; DAXX; KRAS; PRKCD; PRKAA1; MAPK9; CDK2; PIM1; PIK3C2A; TRAF2; TP53;
    LCK; MAP3K7; DYRK1A; MAP2K2; PIK3R1; MAP2K1; PAK3; CDC42; JUN; TTK; CSNK1A1;
    CRKL; BRAF; SGK
    PPAr/RXR Signaling PRKAA2; EP300; INS; SMAD2; TRAF6; PPARA; FASN; RXRA; MAPK1; SMAD3; GNAS;
    IKBKB; NCOR2; ABCA1; GNAQ; NFKB2; MAP3K14; STAT5B; MAPK8; IRS1; MAPK3;
    KRAS; RELA; PRKAA1; PPARGC1A; NCOA3; MAPK14; INSR; RAF1; IKBKG; RELB;
    MAP3K7; CREBBP; MAP2K2; JAK2; CHUK; MAP2K1; NFKB1; TGFBR1; SMAD4; JUN;
    IL1R1; PRKCA; IL6; HSP90AA1; ADIPOQ
    NF-KB Signaling IRAK1; EIF2AK2; EP300; INS; MYD88; PRKCZ: TRAF6; TBK1; AKT2; EGFR; IKBKB;
    PIK3CA; BTRC; NFKB2; MAP3K14; PIK3CB; PIK3C3; MAPK8; RIPK1; HDAC2; KRAS;
    RELA; PIK3C2A; TRAF2; TLR4: PDGFRB; TNF; INSR; LCK; IKBKG; RELB; MAP3K7;
    CREBBP; AKT1; PIK3R1; CHUK; PDGFRA; NFKB1; TLR2; BCL10; GSK3B; AKT3; TNFAIP3; IL1R1
    Neuregulin Signaling Wnt & ERBB4; PRKCE; ITGAM; ITGA5: PTEN; PRKCZ; ELK1; MAPK1; TPN11; AKT2; EGFR; ERBB2;
    Beta catenin PRKCI; CDKN1B; STAT5B; PRKD1; MAPK3; ITGA1; KRAS; PRKCD; STAT5A; SRC; ITGB7;
    Signaling RAF1; ITGB1; MAP2K2; ADAM17; AKT1; PIK3R1; PDPK1; MAP2K1; ITGB3; EREG; FRAP1;
    PSEN1; ITGA2; MYC; NRG1; CRKL; AKT3; PRKCA; HSP90AA1; RPS6KB1 CD44; EP300;
    LRP6; DVL3; CSNK1E; GJA1; SMO; AKT2; PIN1; CDH1; BTRC; GNAQ; MARK2; PPP2R1A;
    WNT11; SRC; DKK1; PPP2CA; SOX6; SFRP2: ILK; LEF1; SOX9; TP53; MAP3K7; CREBBP;
    TCF7L2; AKT1; PPP2R5C; WNT5A; LRP5; CTNNB1; TGFBR1; CCND1; GSK3A; DVL1; APC;
    CDKN2A; MYC; CSNK1A1; GSK3B; AKT3; SOX2
    Insulin Receptor PTEN; INS; EIF4E; PTPN1; PRKCZ; MAPK1; TSC1; PTPN11; AKT2; CBL; PIK3CA; PRKCI;
    Signaling PIK3CB; PIK3C3; MAPK8; IRS1; MAPK3; TSC2; KRAS; EIF4EBP1; SLC2A4; PIK3C2A;
    PPP1CC; INSR; RAF1; FYN; MAP2K2; JAK1; AKT1; JAK2; PIK3R1; PDPK1; MAP2K1;
    GSK3A; FRAP1; CRKL; GSK3B; AKT3; FOXO1; SGK; RPS6KB1
    IL-6 Signaling HSPB1; TRAF6; MAPKAPK2; ELK1; MAPK1; PTPN11; IKBKB; FOS; NFKB2: MAP3K14;
    MAPK8; MAPK3; MAPK10; IL6ST; KRAS; MAPK13; IL6R; RELA; SOCS1; MAPK9;
    ABCB1; TRAF2; MAPK14; TNF; RAF1; IKBKG; RELB; MAP3K7; MAP2K2; IL8; JAK2;
    CHUK; STAT3; MAP2K1; NFKB1; CEBPB; JUN; IL1R1; SRF; IL6
    Hepatic Cholestasis PRKCE; IRAK1; INS; MYD88; PRKCZ; TRAF6; PPARA; RXRA; IKBKB; PRKCI; NFKB2;
    MAP3K14; MAPK8; PRKD1; MAPK10; RELA; PRKCD; MAPK9; ABCB1; TRAF2; TLR4;
    TNF; INSR; IKBKG; RELB; MAP3K7; IL8; CHUK; NR1H2; TJP2; NFKB1; ESR1;
    SREBF1; FGFR4; JUN; IL1R1; PRKCA; IL6
    IGF-1 Signaling IGF1; PRKCZ; ELK1; MAPK1; PTPN11; NEDD4; AKT2; PIK3CA; PRKC1; PTK2;
    FOS; PIK3CB; PIK3C3; MAPK8; 1GF1R; IRS1; MAPK3; IGFBP7; KRAS; PIK3C2A; YWHAZ;
    PXN; RAF1; CASP9; MAP2K2; AKT1; PIK3R1; PDPK1; MAP2K1; IGFBP2; SFN; JUN; CYR61;
    AKT3; FOXO1; SRF; CTGF; RPS6KB1
    NRF2-mediated PRKCE; EP300; SOD2; PRKCZ; MAPK1; SQSTM1; NQO1; PIK3CA; PRKC1; FOS; PIK3CB;
    Oxidative P1K3C3; MAPK8; PRKD1; MAPK3; KRAS; PRKCD; GSTP1; MAPK9; FTL; NFE2L2;
    Stress Response PIK3C2A; MAPK14; RAF1; MAP3K7; CREBBP; MAP2K2; AKT1; PIK3R1; MAP2K1; PPIB;
    JUN; KEAP1; GSK3B; ATF4; PRKCA; EIF2AK3; HSP90AA1
    Hepatic, Fibrosis/Hepatic EDN1; IGF1; KDR; FLT1; SMAD2; FGFR1; MET; PGF; SMAD3; EGFR; FAS; CSF1; NFKB2;
    Stellate Cell Activation BCL2; MYH9; IGF1R; IL6R; RELA; TLR4; PDGFRB; TNF; RELB; IL8; PDGFRA; NFKB1;
    TGFBR1; SMAD4; VEGFA; BAX; IL1R1; CCL2; HGF; MMP1; STAT1; IL6; CTGF; MMP9
    PPAR Signaling EP300; INS; TRAF6; PPARA; RXRA; MAPK1; IKBKB; NCOR2; FOS; NFKB2; MAP3K14;
    STAT5B; MAPK3; NRIP1; KRAS; PPARG; RELA; STAT5A; TRAF2; PPARGC1A; PDGFRB;
    TNF; INSR; RAF1; IKBKG; RELB; MAP3K7; CREBBP; MAP2K2; CHUK; PDGFRA; MAP2K1;
    NFKB1; JUN; IL1R1; HSP90AA1
    Fc Epsilon Rl Signaling PRKCE; RAC1; PRKCZ; LYN; MAPK1; RAC2; PTPN11; AKT2; PIK3CA; SYK; PRKCI;
    PIK3CB; PIK3C3; MAPK8; PRKD1; MAPK3; MAPK10; KRAS; MAPK13; PRKCD; MAPK9;
    PIK3C2A; BTK; MAPK14; TNF; RAF1; FYN; MAP2K2; AKT1; PIK3R1; PDPK1; MAP2K1;
    AKT3; VAV3; PRKCA
    G-Protein Coupled PRKCE; RAP1A; RGS16; MAPK1; GNAS; AKT2; IKBKB; PIK3CA; CREB1; GNAQ; NFKB2;
    Receptor Signaling CAMK2A; PIK3CB; PIK3C3; MAPK3; KRAS; RELA; SRC; PIK3C2A; RAF1; IKBKG; RELB;
    FYN; MAP2K2; AKT1; PIK3R1; CHUK; PDPK1; STAT3; MAP2K1; NFKB1; BRAF; ATF4; AKT3; PRKCA
    Inositol Phosphate PRKCE; IRAK1; PRKAA2; EIF2AK2; PTEN; GRK6; MAPK1; PLK1; AKT2; PIK3CA; CDK8;
    Metabolism PIK3CB; PIK3C3; MAPK8; MAPK3; PRKCD; PRKAA1; MAPK9; CDK2; PIM1; PIK3C2A;
    DYRK1A; MAP2K2; PIP5K1A; PIK3R1; MAP2K1; PAK3; ATM; TTK; CSNK1A1; BRAF; SGK
    PDGF Signaling EIF2AK2; ELK1; ABL2; MAPK1; PIK3CA; FOS; PIK3CB; PIK3C3; MAPK8; CAV1; ABL1;
    MAPK3; KRAS; SRC; PIK3C2A; PDGFRB; RAF1; MAP2K2; JAK1; JAK2; PIK3R1; PDGFRA;
    STAT3; SPHK1; MAP2K1; MYC; JUN; CRKL; PRKCA; SRF; STAT1; SPHK2
    VEGF Signaling ACTN4; ROCK1; KDR; FLT1; ROCK2; MAPK1; PGF; AKT2; PIK3CA; ARNT; PTK2; BCL2;
    PIK3CB; PIK3C3; BCL2L1; MAPK3; KRAS; HIF1A; NOS3; PIK3C2A; PXN; RAF1;
    MAP2K2; ELAVL1; AKT1; PIK3R1; MAP2K1; SFN; VEGFA; AKT3; FOXO1; PRKCA
    Natural Killer Cell PRKCE; RAC1; PRKCZ; MAPK1; RAC2; PTPN11; KIR2DL3; AKT2; PIK3CA; SYK;
    Signaling PRKCI; PIK3CB; PIK3C3; PRKD1; MAPK3; KRAS; PRKCD; PTPN6; PIK3C2A;
    LCK; RAF1; FYN; MAP2K2; PAK4; AKT1; PIK3R1; MAP2K1; PAK3; AKT3; VAV3; PRKCA
    Cell Cycle: G1/S HDAC4; SMAD3; SUV39H1; HDAC5; CDKN1B; BTRC; ATR; ABL1; E2F1; HDAC2; HDAC7A;
    Checkpoint Regulation RB1; HDAC11; HDAC9; CDK2; E2F2; HDAC3; TP53; CDKN1A; CCND1; E2F4; ATM; RBL2;
    SMAD4; CDKN2A; MYC; NRG1; GSK3B; RBL1; HDAC6
    T Cell Receptor RAC1; ELK1; MAPK1; IKBKB; CBL; PIK3CA; FOS; NFKB2; PIK3CB; PIK3C3; MAPK8; MAPK3;
    Signaling KRAS; RELA, PIK3C2A; BTK; LCK; RAF1; IKBKG; RELB, FYN; MAP2K2; PIK3R1;
    CHUK; MAP2K1; NFKB1; ITK; BCL10; JUN; VAV3
    Death Receptor Signaling CRADD; HSPB1; BID; BIRC4; TBK1; IKBKB; FADD; FAS; NFKB2; BCL2; MAP3K14;
    MAPK8; RIPK1; CASP8; DAXX; TNFRSF10B; RELA; TRAF2; TNF; IKBKG; RELB;
    CASP9; CHUK; APAF1; NFKB1; CASP2; BIRC2; CASP3; BIRC3
    FGF Signaling RAC1; FGFR1; MET; MAPKAPK2; MAPK1; PTPN11; AKT2; PIK3CA; CREB1; PIK3CB;
    PIK3C3; MAPK8; MAPK3; MAPK13; PTPN6; PIK3C2A; MAPK14; RAF1; AKT1; PIK3R1;
    STAT3; MAP2K1; FGFR4; CRKL; ATF4; AKT3; PRKCA; HGF
    GM-CSF Signaling LYN; ELK1; MAPK1; PTPN11; AKT2; PIK3CA; CAMK2A; STAT5B; PIK3CB; PIK3C3;
    GNB2L1; BCL2L1; MAPK3; ETS1; KRAS; RUNX1; PIM1; PIK3C2A; RAF1; MAP2K2; AKT1;
    JAK2; PIK3R1; STAT3; MAP2K1; CCND1; AKT3; STAT1
    Amyotrophic Lateral BID; IGF1; RAC1; BIRC4; PGF; CAPNS1; CAPN2; PIK3CA; BCL2; PIK3CB; PIK3C3;
    Sclerosis Signaling BCL2L1; CAPN1; PIK3C2A; TP53; CASP9; PIK3R1; RAB5A; CASP1; APAF1; VEGFA;
    BIRC2; BAX; AKT3; CASP3; BIRC3
    JAK/Stat Signaling PTPN1; MAPK1; PTPN11; AKT2; PIK3CA; STAT5B; PIK3CB; PIK3C3; MAPK3; KRAS; SOCS1;
    STAT5A; PTPN6; PIK3C2A; RAF1; CDKN1A; MAP2K2; JAK1; AKT1; JAK2; PIK3R1; STAT3;
    MAP2K1; FRAP1; AKT3; STAT1
    Nicotinate and PRKCE; IRAK1; PRKAA2; EIF2AK2; GRK6; MAPK1; PLK1; AKT2; CDK8; MAPK8; MAPK3; PRKCD;
    Nicotinamide PRKAA1; PBEF1; MAPK9; CDK2; PIM1; DYRK1A; MAP2K2; MAP2K1; PAK3; NT5E; TTK; CSNK1A1;
    Metabolism BRAF; SGK
    Chemokine Signaling CXCR4; ROCK2; MAPK1; PTK2; FOS; CFL1; GNAQ; CAMK2A; CXCL12; MAPK8; MAPK3;
    KRAS; MAPK13; RHOA; CCR3; SRC; PPP1CC; MAPK14; NOX1; RAF1; MAP2K2; MAP2K1;
    JUN; CCL2; PRKCA
    IL-2 Signaling ELK1; MAPK1; PTPN11; AKT2; PIK3CA; SYK; FOS; STAT5B; PIK3CB; PIK3C3; MAPK8;
    MAPK3; KRAS; SOCS1; STAT5A; PIK3C2A: LCK; RAF1; MAP2K2; JAK1; AKT1; PIK3R1;
    MAP2K1; JUN; AKT3
    Synaptic Long Term PRKCE; IGF1; PRKCZ; PRDX6; LYN; MAPK1; GNAS; PRKC1; GNAQ; PPP2R1A; IGF1R;
    Depression PRKID1; MAPK3; KRAS; GRN; PRKCD; NOS3; NOS2A; PPP2CA; YWHAZ; RAF1; MAP2K2;
    PPP2R5C; MAP2K1; PRKCA
    Estrogen Receptor TAF4B; EP300; CARM1; PCAF; MAPK1; NCOR2; SMARCA4; MAPK3; NRIP1; KRAS; SRC;
    Signaling NR3C1; HDAC3; PPARGC1A; RBM9; NCOA3; RAF1; CREBBP; MAP2K2; NCOA2; MAP2K1;
    PRKDC; ESR1; ESR2
    Protein Ubiquitination TRAF6; SMURF1; BIRC4; BRCA1; UCHL1; NEDD4; CBL; UBE2I; BTRC; HSPA5; USP7;
    Pathway USP10; FBXW7; USP9X; STUB1; USP22; B2M; BIRC2; PARK2; USP8; USP1; VHL;
    HSP90AA1; BIRC3
    IL-10 Signaling TRAF6; CCR1; ELK1; IKBKB; SP1; FOS; NFKB2; MAP3K14; MAPK8; MAPK13; RELA;
    MAPK14; TNF; IKBKG; RELB; MAP3K7; JAK1; CHUK; STAT3; NFKB1; JUN; IL1R1; IL6
    VDR/RXR Activation PRKCE; EP300; PRKCZ; RXRA; GADD45A; HES1; NCOR2; SP1; PRKC1; CDKN1B; PRKD1;
    PRKCD; RUNX2; KLF4; YY1; NCOA3; CDKN1A; NCOA2; SPP1; LRP5; CEBPB; FOXO1; PRKCA
    TGF-beta Signaling EP300; SMAD2; SMURF1; MAPK1; SMAD3; SMAD1; FOS; MAPK8; MAPK3; KRAS; MAPK9;
    RUNX2; SERPINE1; RAF1; MAP3K7; CREBBP; MAP2K2; MAP2K1; TGFBR1; SMAD4; JUN; SMAD5
    Toll-like Receptor IRAK1; EIF2AK2; MYD88; TRAF6; PPARA; ELK1; IKBKB; FOS; NFKB2; MAP3K14; MAPK8;
    Signaling MAPK13; RELA; TLR4; MAPK14; IKBKG; RELB; MAP3K7; CHUK; NFKB1; TLR2; JUN
    p38 MAPK Signaling HSPB1; IRAK1; TRAF6; MAPKAPK2; ELK1; FADD; FAS; CREB1; DDIT3; RPS6KA4;
    DAXX; MAPK13; TRAF2; MAPK14; TNF; MAP3K7; TGFBR1; MYC; ATF4; IL1R1; SRF; STAT1
    Neurotrophin/TRK NTRK2; MAPK1; PTPN11; PIK3CA; CREB1; FOS; PIK3CB; PIK3C3; MAPK8; MAPK3; KRAS;
    Signaling PIK3C2A; RAF1; MAP2K2; AKT1; PIK3R1; PDPK1; MAP2K1; CDC42; JUN; ATF4
    FXR/RXR Activation INS; PPARA; FASN; RXRA; AKT2; SDC1; MAPK8; APOB; MAPK10; PPARG; MTTP;
    MAPK9; PPARGC1A; TNF; CREBBP; AKT1; SREBF1; FGFR4; AKT3; FOXO1
    Synaptic Long Term PRKCE; RAP1A; EP300; PRKCZ; MAPK1; CREB1; PRKC1; GNAQ; CAMK2A; PRKD1;
    Potentiation MAPK3; KRAS; PRKCD; PPP1CC; RAF1; CREBBP; MAP2K2; MAP2K1; ATF4; PRKCA
    Calcium Signaling RAP1A; EP300; HDAC4; MAPK1; HDAC5; CREB1; CAMK2A; MYH9; MAPK3; HDAC2;
    HDAC7A; HDAC11; HDAC9; HDAC3; CREBBP; CALR; CAMKK2; ATF4; HDAC6
    EGF Signaling ELK1; MAPK1; EGFR; PIK3CA; FOS; PIK3CB; PIK3C3; MAPK8; MAPK3; PIK3C2A; RAF1;
    JAK1; PIK3R1; STAT3; MAP2K1; JUN; PRKCA; SRF; STAT1
    Hypoxia Signaling in the EDN1; PTEN; EP300; NQO1; UBE21; CREB1; ARNT; HIF1A; SLC2A4; NOS3; TP53; LDHA;
    Cardiovascular System AKT1; ATM; VEGFA; JUN; ATF4; VHL; HSP90AA1
    LPS/IL-1 Mediated IRAK1; MYD88; TRAF6; PPARA; RXRA; ABCA1, MAPK8; ALDH1A1; GSTP1; MAPK9; ABCB1;
    Inhibition of RXR Function TRAF2; TLR4; TNF; MAP3K7; NR1H2; SREBF1; JUN; IL1R1
    LXR/RXR Activation FASN; RXRA; NCOR2; ABCA1; NFKB2; IRF3; RELA; NOS2A; TLR4; TNF; RELB; LDLR;
    NR1H2; NFKB1; SREBF1; IL1R1; CCL2; IL6; MMP9
    Amyloid Processing PRKCE; CSNK1E; MAPK1; CAPNS1; AKT2; CAPN2; CAPN1; MAPK3; MAPK13; MAPT;
    MAPK14; AKT1; PSEN1; CSNK1A1; GSK3B; AKT3; APP
    IL-4 Signaling AKT2; PIK3CA; PIK3CB; PIK3C3; IRS1; KRAS; SOCS1; PTPN6; NR3C1; PIK3C2A;
    JAK1; AKT1; JAK2; PIK3R1; FRAP1; AKT3; RPS6KB1
    Cell Cycle: G2/M DNA EP300; PCAF; BRCA1; GADD45A; PLK1;
    Damage Checkpoint BTRC; CHEK1; ATR; CHEK2; YWHAZ; TP53; CDKN1A; PRKDC; ATM; SFN; CDKN2A
    Regulation
    Nitric Oxide Signaling KDR; FLT1; PGF; AKT2; PIK3CA; PIK3CB; PIK3C3; CAV1; PRKCD; NOS3; PIK3C2A;
    (Cardiovascular System) AKT1; PIK3R1; VEGFA; AKT3; HSP90AA1
    Purine Metabolism NME2; SMARCA4; MYH9; RRM2; ADAR; EIF2AK4; PKM2; ENTPD1; RAD51; RRM2B;
    TJP2; RAD51C; NT5E; POLD1; NME1
    cAMP-mediated Signaling RAP1A; MAPK1; GNAS; CREB1; CAMK2A; MAPK3; SRC; RAF1; MAP2K2; STAT3; MAP2K1; BRAF; ATF4
    Mitochondrial SOD2; MAPK8; CASP8; MAPK10; MAPK9;
    Dysfunction CASP9; PARK7; PSEN1; PARK2; APP; CASP3
    Notch Signaling HES1; JAG1; NUMB; NOTCH4; ADAM17; NOTCH2; PSEN1; NOTCH3; NOTCH1; DLL4
    Endoplasmic Reticulum HSPA5; MAPK8; XBP1; TRAF2; ATF6; CASP9; ATF4; EIF2AK3; CASP3
    Stress Pathway
    Pyrimidine Metabolism NME2; AICDA; RRM2; EIF2AK4; ENTPD1; RRM2B; NT5E; POLD1; NME1
    Parkinson's Signaling UCHL1; MAPK8; MAPK13; MAPK14; CASP9; PARK7; PARK2; CASP3
    Cardiac & Beta GNAS; GNAQ; PPP2R1A; GNB2L1; PPP2CA; PPP1CC; PPP2R5C
    Adrenergic Signaling
    Glycolysis/Gluconeogenesis HK2; GCK; GPI; ALDH1A1; PKM2; LDHA; HK1
    Interferon Signaling IRF1; SOCS1; JAK1; JAK2; IFITM1; STAT1; IFIT3
    Sonic Hedgehog Signaling ARRB2; SMO; GLI2; DYRK1A; GLI1; GSK3B; DYRKIB
    Glycerophospholipid PLD1; GRN; GPAM; YWHAZ; SPHK1; SPHK2
    Metabolism
    Phospholipid PRDX6; PLD1; GRN; YWHAZ; SPHK1; SPHK2
    Degradation
    Tryptophan Metabolism SIAH2; PRMT5; NEDD4; ALDH1A1; CYP1B1; SIAH1
    Lysine Degradation SUV39H1; EHMT2; NSD1; SETD7; PPP2R5C
    Nucleotide Excision ERCC5; ERCC4; XPA; XPC; ERCC1
    Repair Pathway
    Starch and Sucrose UCHL1; HK2; GCK; GPI; HK1
    Metabolism
    Aminosugars Metabolism NQO1; HK2; GCK; HK1
    Arachidonic Acid PRDX6; GRN; YWHAZ; CYP1B1
    Metabolism
    Circadian Rhythm CSNK1E; CREB1; ATF4; NR1D1
    Signaling
    Coagulation System BDKRB1; F2R; SERPINE1; F3
    Dopamine Receptor PPP2R1A; PPP2CA; PPP1CC; PPP2R5C
    Signaling
    Glutathione Metabolism IDH2; GSTP1; ANPEP; IDH1
    Glycerolipid Metabolism ALDH1A1; GPAM; SPHK1; SPHK2
    Linoleic Acid Metabolism PRDX6; GRN; YWHAZ; CYP1B1
    Methionine Metabolism DNMT1; DNMT3B; AHCY; DNMT3A
    Pyruvate Metabolism GLO1; ALDH1A1; PKM2; LDHA
    Arginine and Proline ALDH1A1; NOS3; NOS2A
    Metabolism
    Eicosanoid Signaling PRDX6; GRN; YWHAZ
    Fructose and Mannose HK2; GCK; HK1
    Metabolism
    Galactose Metabolism HK2; GCK; HK1
    Stilbene, Coumarine and PRDX6; PRDX1; TYR
    Lignin Biosynthesis
    Antigen Presentation CALR; B2M
    Pathway
    Biosynthesis of Steroids NQO1; DHCR7
    Butanoate Metabolism ALDH1A1; NLGN1
    Citrate Cycle IDH2; IDH1
    Fatty Acid Metabolism ALDH1A1; CYP1B1
    Glycerophospholipid PRDX6; CHKA
    Metabolism
    Histidine Metabolism PRMT5; ALDH1A1
    Inositol Metabolism ERO1L; APEX1
    Metabolism of Xenobiotics GSTP1; CYP1B1
    by Cytochrome p450
    Methane Metabolism PRDX6; PRDX1
    Phenylalanine Metabolism PRDX6; PRDX1
    Propanoate Metabolism ALDH1A1; LDHA
    Selenoamino Acid PRMT5; AHCY
    Metabolism
    Sphingolipid Metabolism SPHK1; SPHK2
    Aminophosphonate PRMT5
    Metabolism
    Androgen and Estrogen PRMT5
    Metabolism
    Ascorbate and Aldarate ALDH1A1
    Metabolism
    Bile Acid Biosynthesis ALDH1A1
    Cysteine Metabolism LDHA
    Fatty Acid Biosynthesis FASN
    Glutamate Receptor GNB2L1
    Signaling
    NRF2-mediated PRDX1
    Oxidative Stress Response
    Pentose Phosphate GPI
    Pathway
    Pentose and Glucuronate UCHL1
    Interconversions
    Retinol Metabolism ALDH1A1
    Riboflavin Metabolism TYR
    Tyrosine Metabolism PRMT5, TYR
    Ubiquinone Biosynthesis PRMT5
    Valine, Leucine and ALDH1A1
    Isoleucine Degradation
    Glycine, Serine and CHKA
    Threonine Metabolism
    Lysine Degradation ALDH1A1
    Pain/Taste TRPM5; TRPA1, TRPM7; TRPC5; TRPC6; TRPC1; Cnr1; cnr2; Grk2; Trpa1; Pomc;
    Pain Cgrp; Crf; Pka; Era; Nr2b; TRPM5; Prkaca; Prkacb; Prkar1a; Prkar2a
    Mitochondrial Function AIF; CytC; SMAC (Diablo); Aifm-1; Aifm-2, BMP-4; Chordin (Chrd); Noggin (Nog);
    Developmental WNT, (Wnt2; Wnt2b; Wnt3a; Wnt4; Wnt5a; Wnt6; Wnt7b; Wnt8b; Wnt9a; Wnt9b;
    Neurology Wnt10a; Wnt10b; Wnt16); beta-catenin; Dkk-1; Frizzled related proteins; Otx-2;
    Gbx2; FGF-8; Reelin; Dab1; unc-86 (Pou4f1 or Brn3a); Numb; Reln
  • Embodiments of the invention also relate to methods and compositions related to knocking out genes, amplifying genes and repairing particular mutations, including point mutations, frame shift mutations, deletions or insertions.
  • In yet another aspect of the invention, the invention may be used to correct ocular defects that arise from several genetic mutations.
  • Several further aspects of the invention relate to correcting defects associated with a wide range of genetic diseases which are further described on the website of the National Institutes of Health under the topic subsection Genetic Disorders (website at health.nih.gov/topic/GeneticDisorders). The genetic brain diseases may include but are not limited to Adrenoleukodystrophy, Agenesis of the Corpus Callosum, Aicardi Syndrome, Alpers' Disease. Alzheimer's Disease, Barth Syndrome, Batten Disease, CADASIL, Cerebellar Degeneration, Fabry's Disease, Gerstmann-Straussler-Scheinker Disease, Huntington's Disease and other Triplet Repeat Disorders, Leigh's Disease, Lesch-Nyhan Syndrome, Menkes Disease, Mitochondrial Myopathies and NINDS Colpocephaly. These diseases are further described on the website of the National Institutes of Health under the subsection Genetic Brain Disorders.
  • In some embodiments, the condition may be neoplasia. In some embodiments, where the condition is neoplasia, the genes to be targeted are any of those listed in Table A (in this case PTEN asn so forth). In some embodiments, the condition may be Age-related Macular Degeneration. In some embodiments, the condition may be a Schizophrenic Disorder. In some embodiments, the condition may be a Trinucleotide Repeat Disorder. In some embodiments, the condition may be Fragile X Syndrome. In some embodiments, the condition may be a Secretase Related Disorder. In some embodiments, the condition may be a Prion—related disorder. In some embodiments, the condition may be ALS. In some embodiments, the condition may be a drug addiction. In some embodiments, the condition may be Autism. In some embodiments, the condition may be Alzheimer's Disease. In some embodiments, the condition may be inflammation. In some embodiments, the condition may be Parkinson's Disease.
  • Examples of proteins associated with Parkinson's disease include but are not limited to α-synuclein, DJ-1, LRRK2, PINK1, Parkin, UCHL1, Synphilin-1, and NURR1.
  • Examples of addiction-related proteins may include ABAT for example.
  • Examples of inflammation-related proteins may include the monocyte chemoattractant protein-1 (MCP1) encoded by the Ccr2 gene, the C—C chemokine receptor type 5 (CCR5) encoded by the Ccr5 gene, the IgG receptor IIB (FCGR2b, also termed CD32) encoded by the Fcgr2b gene, or the Fc epsilon R1 g (FCER1g) protein encoded by the Fcerlg gene, for example.
  • Examples of cardiovascular diseases associated proteins may include IL1B (interleukin 1, beta), XDH (xanthine dehydrogenase), TP53 (tumor protein p53), PTGIS (prostaglandin 12 (prostacyclin) synthase), MB (myoglobin), IL4 (interleukin 4), ANGPT1 (angiopoietin 1), ABCG8 (ATP-binding cassette, sub-family G (WHITE), member 8), or CTSK (cathepsin K), for example.
  • Examples of Alzheimer's disease associated proteins may include the very low density lipoprotein receptor protein (VLDLR) encoded by the VLDLR gene, the ubiquitin-like modifier activating enzyme 1 (UBA1) encoded by the UBA1 gene, or the NEDD8-activating enzyme E1 catalytic subunit protein (UBE1C) encoded by the UBA3 gene, for example.
  • Examples of proteins associated Autism Spectrum Disorder may include the benzodiazapine receptor (peripheral) associated protein 1 (BZRAP1) encoded by the BZRAP1 gene, the AF4/FMR2 family member 2 protein (AFF2) encoded by the AFF2 gene (also termed MFR2), the fragile X mental retardation autosomal homolog 1 protein (FXR1) encoded by the FXR1 gene, or the fragile X mental retardation autosomal homolog 2 protein (FXR2) encoded by the FXR2 gene, for example.
  • Examples of proteins associated Macular Degeneration may include the ATP-binding cassette, sub-family A (ABC1) member 4 protein (ABCA4) encoded by the ABCR gene, the apolipoprotein E protein (APOE) encoded by the APOE gene, or the chemokine (C—C motif) Ligand 2 protein (CCL2) encoded by the CCL2 gene, for example.
  • Examples of proteins associated Schizophrenia may include NRG1, ErbB4, CPLX1, TPH1, TPH2, NRXN1, GSK3A, BDNF, DISCI, GSK3B, and combinations thereof.
  • Examples of proteins involved in tumor suppression may include ATM (ataxia telangiectasia mutated), ATR (ataxia telangiectasia and Rad3 related), EGFR (epidermal growth factor receptor), ERBB2 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2), ERBB3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3), ERBB4 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 4), Notch 1, Notch2, Notch 3, or Notch 4, for example. Examples of proteins associated with a secretase disorder may include PSENEN (presenilin enhancer 2 homolog (C. elegans)), CTSB (cathepsin B), PSEN1 (presenilin 1), APP (amyloid beta (A4) precursor protein), APH1B (anterior pharynx defective 1 homolog B (C. elegans)), PSEN2 (presenilin 2 (Alzheimer disease 4)), or BACE1 (beta-site APP-cleaving enzyme 1), for example.
  • Examples of proteins associated with Amyotrophic Lateral Sclerosis may include SOD1 (superoxide dismutase 1), ALS2 (amyotrophic lateral sclerosis 2), FUS (fused in sarcoma), TARDBP (TAR DNA binding protein), VAGFA (vascular endothelial growth factor A), VAGFB (vascular endothelial growth factor B), and VAGFC (vascular endothelial growth factor C), and any combination thereof.
  • Examples of proteins associated with prion diseases may include SODI (superoxide dismutase 1), ALS2 (amyotrophic lateral sclerosis 2), FUS (fused in sarcoma), TARDBP (TAR DNA binding protein), VAGFA (vascular endothelial growth factor A), VAGFB (vascular endothelial growth factor B), and VAGFC (vascular endothelial growth factor C), and any combination thereof.
  • Examples of proteins related to neurodegenerative conditions in prion disorders may include A2M (Alpha-2-Macroglobulin), AATF (Apoptosis antagonizing transcription factor), ACPP (Acid phosphatase prostate), ACTA2 (Actin alpha 2 smooth muscle aorta), ADAM22 (ADAM metallopeptidase domain), ADORA3 (Adenosine A3 receptor), or ADRA1D (Alpha-1D adrenergic receptor for Alpha-1D adrenoreceptor), for example.
  • Examples of proteins associated with Immunodeficiency may include A2M [alpha macroglobulin]; AANAT [arylalkylamine N-acetyltransferase]; ABCA1 [ATP-binding cassette, sub-family A (ABC1), member 1]; ABCA2 [ATP-binding cassette, sub-family A (ABC1), member 2]; or ABCA3 [ATP-binding cassette, sub-family A (ABC1), member 3]; for example. Examples of proteins associated with Trinucleotide Repeat Disorders include AR (androgen receptor), FMR1 (fragile X mental retardation 1), HTT (huntingtin), or DMPK (dystrophia myotonica-protein kinase), FXN (frataxin), ATXN2 (ataxin 2). for example.
  • Examples of proteins associated with Neurotransmission Disorders include SST (somatostatin), NOS1 (nitric oxide synthase 1 (neuronal)), ADRA2A (adrenergic, alpha-2A-, receptor), ADRA2C (adrenergic, alpha-2C-, receptor), TACR1 (tachykinin receptor 1), or HTR2c (5-hydroxytryptamine (serotonin) receptor 2C), for example.
  • Examples of neurodevelopmental-associated sequences include A2BP1 [ataxin 2-binding protein 1], AADAT [aminoadipate aminotransferase], AANAT [arylalkylamine N-acetyltransferase], ABAT [4-aminobutyrate aminotransferase], ABCA1 [ATP-binding cassette, sub-family A (ABC1), member 1], or ABCA13 [ATP-binding cassette, sub-family A (ABC1), member 13], for example.
  • Further examples of preferred conditions treatable with the present invention include may be selected from: Aicardi-Goutières Syndrome; Alexander Disease; Allan-Herndon-Dudley Syndrome; POLG-Related Disorders; Alpha-Mannosidosis (Type II and III); Alström Syndrome; Angelman; Syndrome; Ataxia-Telangiectasia; Neuronal Ceroid-Lipofuscinoses; Beta-Thalassemia; Bilateral Optic Atrophy and (Infantile) Optic Atrophy Type 1; Retinoblastoma (bilateral); Canavan Disease; Cerebrooculofacioskeletal Syndrome 1 [COFS1]; Cerebrotendinous Xanthomatosis; Cornelia de Lange Syndrome; MAPT-Related Disorders; Genetic Prion Diseases; Dravet Syndrome; Early-Onset Familial Alzheimer Disease; Friedreich Ataxia [FRDA]; Fryns Syndrome; Fucosidosis; Fukuyama Congenital Muscular Dystrophy; Galactosialidosis; Gaucher Disease; Organic Acidemias; Hemophagocytic Lymphohistiocytosis; Hutchinson-Gilford Progeria Syndrome; Mucolipidosis II; Infantile Free Sialic Acid Storage Disease; PLA2G6-Associated Neurodegeneration; Jervell and Lange-Nielsen Syndrome; Junctional Epidermolysis Bullosa; Huntington Disease; Krabbe Disease (Infantile); Mitochondrial DNA-Associated Leigh Syndrome and NARP; Lesch-Nyhan Syndrome; LIS1-Associated Lissencephaly; Lowe Syndrome; Maple Syrup Urine Disease; MECP2 Duplication Syndrome; ATP7A-Related Copper Transport Disorders; LAMA2-Related Muscular Dystrophy; Arylsulfatase A Deficiency; Mucopolysaccharidosis Types I, II or III; Peroxisome Biogenesis Disorders, Zellweger Syndrome Spectrum; Neurodegeneration with Brain Iron Accumulation Disorders; Acid Sphingomyelinase Deficiency; Niemann-Pick Disease Type C; Glycine Encephalopathy; ARX-Related Disorders; Urea Cycle Disorders; COL1A 1/2-Related Osteogenesis Imperfecta; Mitochondrial DNA Deletion Syndromes; PLP1-Related Disorders; Perry Syndrome; Phelan-McDermid Syndrome; Glycogen Storage Disease Type II (Pompe Disease) (Infantile); MAPT-Related Disorders; MECP2-Related Disorders; Rhizomelic Chondrodysplasia Punctata Type 1; Roberts Syndrome; Sandhoff Disease; Schindler Disease—Type 1; Adenosine Deaminase Deficiency; Smith-Lemli-Opitz Syndrome; Spinal Muscular Atrophy, Infantile-Onset Spinocerebellar Ataxia; Hexosaminidase A Deficiency; Thanatophoric Dysplasia Type 1; Collagen Type VI-Related Disorders; Usher Syndrome Type I; Congenital Muscular Dystrophy; Wolf-Hirschhorn Syndrome; Lysosomal Acid Lipase Deficiency; and Xeroderma Pigmentosum.
  • As will be apparent, it is envisaged that the present system can be used to target any polynucleotide sequence of interest. Some examples of conditions or diseases that might be usefully treated using the present system are listed above and examples of genes currently associated with those conditions are also provided there. However, the genes exemplified are not exhaustive.
    • EXAMPLE F-9
  • In a preferred embodiment for DRiGR the designer DNA-recombining enzyme according to the invention replaces a mutation in exon 8 of the F9 gene that is responsible for hemophilia B. Exon 8 is frequently mutated in severe forms of hemophilia B and mutations that occur in the population are known (www.factorix.org).
  • In one embodiment, the F9 designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 1 (Rec F9-1) or a sequence with a least 90% sequence identity, more preferably 95 sequence identity to SEQ ID NO. 1 and a monomer with the sequence according to SEQ ID NO. 2 (Rec F9-2) or a sequence with a least 80% sequence identity, preferably 90 sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 2.
  • An object of the invention are also designer DNA-recombining enzyme comprising the sequence according to SEQ ID NO. 1 (Rec F9-1) or SEQ ID NO. 2 (Rec F9-2) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 1 or 2.
  • SEQ ID ID NO. 1 (Rec F9-1):
    MSNLQTLHQN LSALLVXAXS DXARKNLMDX FRDRQAFSEH TWXVLLSVCR SWAAWCKLNX  60
    RKWFPAEXXD VRDYLLHLQA XGLXVNTIXQ HLXQLNMLHR RSGLPRPGDS NAVSLVMRRI 120
    RKENVDAGER VKQALAFERX DFDQVRSLME NSDRCQDIRN LAFLGXAYNT LLRISEXARI 180
    RXXDIXRTDG GRMLIHIGRT KTLVSXAGVE KALSLRVTRL VXRWXSVSGV ADDPNNXLXC 240
    RVRRNGVAXP SATSQLSTXX LQGXFAAAHR LIYGARDXSG QRYXTWSGHS ARVGAARDMA 300
    RAGVSIAEIM QAGGWTTXES VMNYIRNLDS ETGAMVRLLE DXD 343
  • In the monomer with the sequence according to SEQ ID NO. 1 (Rec F9-1) preferably the following positions bear the following amino acids residues:
  • X17 is any amino acid, preferably D or G
    X19 is any amino acid, preferably T or A
    X22 is any amino acid, preferably G or E
    X30 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A
    X43 is positively charged amino acid, preferably K or R,
    X60 is any amino acid, preferably N or D
    X68 is any amino acid, preferably P or S
    X69 is a positively or negatively charged amino acid, preferably selected form D, E, K or R, more preferably E or K,
    X81 is a polar amino acid, preferably selected form S, T, C, K or R, more preferably C or R,
    X84 is any amino acid, preferably A or T
    X89 is any amino acid, preferably L or Q
    X93 is a small amino acid, preferably selected form C, S and A, more preferably C or A,
    X140 is any amino acid, preferably I or T
    X166 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or V,
    X177 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or V,
    X182 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or V,
    X183 is positively charged amino acid, preferably K or R,
    X186 is a polar amino acid, preferably S or T
    X206 is any amino acid, preferably A or T
    X222 is any amino acid, preferably V or E
    X225 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or V,
    X237 is a polar amino acid, preferably selected from S, T, C or Y, more preferably Y or C
    X239 is an unpolar amino acid, preferably selected from I, L, V or F, more preferably F or I
    X249 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A
    X259 is any amino acid, preferably F or P
    X260 is any amino acid, preferably T or A
    X264 is any amino acid, preferably A or D
    X278 is any amino acid, preferably L or Q
    X284 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or V,
    X318 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or L,
    X342 is any amino acid, preferably G or D
  • The capital letters stand for the single letter code according to the IUPAC nomenclature. Preferred sequences according SEQ ID No 1 are selected from sequences according to SEQ ID No 28, SEQ ID No 3 and SEQ ID No 5 and sequences with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID No. 28, SEQ ID No 3 or SEQ ID No 5.
  • SEQ ID ID NO. 2 (Rec F9-2):
    MSXLXTLXQN LSAXLXDXXX XEARKNLMDV XRDRQAFSXH TWRVLLSVCR SWAAWCELNN  60
    RKWFPAEPED VRDYLLHLQX RGLXVNT1QQ HLXQLNXLHR RSGLPRPGDS NAVSLVMRRI 120
    RKENXDAGER VXQALAFERT DFDQVRSLME NSDRCQDIRN LAFLGVAYNT LLRISEIARI 180
    RXXDIXRTDG GRMLXHIGRT KTLVSXAGVE KALSLXVTKL VERWISVSGV ADDPNNYLFC 240
    RVRRNGVAXP SAXSQLSTXX LQGXFXAAHR LIXGAXDXSG QRYLTWSGHS ARVGAARDMA 300
    RAGVSXAEIM QAGGWTTVES VMNYXRNLDS ETGAMVRLLE DGD 343
  • In the monomer with the sequence according to SEQ ID NO. 2 (Rec F9-2) preferably the following positions bear the following amino acids residues:
  • X3 is any amino acid, preferably K or N
    X5 is any amino acid, preferably P or Q
    X8 is any amino acid, preferably H or T
    X14 is an unpolar amino acid, preferably selected from I, L or V, more preferably I or L,
    X16 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A
    X18 is small amino acid, preferably A or G
    X19 is any amino acid, preferably T or A
    X20 is a polar amino acid, preferably selected from S, T, N or Q, more preferably S or N
    X21 is any amino acid, preferably D or G
    X31 is an unpolar amino acid, preferably selected form I, L, V and F, more preferably L or F
    X39 is a positively or negatively charged amino acid, preferably selected form D, E, K or R, more preferably K or E,
    X80 is any amino acid, preferably T or A
    X84 is any amino acid, preferably T or A
    X93 is any amino acid, preferably C or A
    X97 is an unpolar amino acid, preferably selected form I, L, V and M, more preferably L or M
    X125 is an unpolar amino acid, preferably selected form I, L and V, more preferably I or V
    X 132 is positively charged amino acid, preferably K or R
    X182 is an unpolar amino acid, preferably selected form I, L and V, more preferably I or V
    X 183 is positively charged amino acid, preferably K or R
    X186 is a polar amino acid, preferably T or S,
    X195 is an unpolar amino acid, preferably selected form I, L and V, more preferably I or V
    X206 is any amino acid, preferably A or T
    X216 is any amino acid, preferably G or R
    X249 is a small unpolar amino acid, preferably selected from V, L, I, A or G, more preferably V or A
    X253 is any amino acid, preferably I or T
    X259 is any amino acid, preferably P or S
    X260 is any amino acid, preferably A or T
    X264 is an unpolar amino acid, preferably selected form I, L and V, more preferably V or I (or L)
    X266 is small amino acid, preferably A or G
    X273 is an aromatic or heteroaromatic amino acid, preferably H or Y
    X276 is any amino acid, preferably K or Q
    X278 is any amino acid, preferably A or D
    X306 is an unpolar amino acid, preferably selected form I, L and V, more preferably L or I
    X325 is an unpolar amino acid, preferably selected form I, L and V, more preferably L or I
  • Again the capital letters stand for the single letter code according to the IUPAC nomenclature.
  • Preferred sequences according SEQ ID No 2 are selected from sequences according to SEQ ID no. 29, SEQ ID No 4 and SEQ ID No 6 and sequences with a least 85% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID No. 29, SEQ ID No 4 or SEQ ID No 6.
  • Preferred pairs of monomers to form the F9 designer DNA-recombining enzyme according to the invention bear the amino acids sequences according to SEQ 28 and 29, and in particular SEQ 3 and 4 as well as SEQ 5 and 6.
  • In another embodiment, the F9 designer DNA-recombining enzyme comprises a sequence according to SEQ ID No. 36 (Rec F9-3) or a sequence with a least 90% sequence identity, preferably 95%, more preferably 98% sequence identity to SEQ ID NO. 36. In this case, the F9 designer DNA-recombining enzyme is preferably a homotetramer.
  • The F9 designer DNA-recombining enzyme according to the invention has been obtained using the method according to the invention described herein and recognizes the asymmetric loxF9-A and loxF9-A target sites according SEQ ID No 7 and SEQ ID No 8:
  • SEQ
    ID
    Name Sequence No.
    loxF9a CTCATTACATTTA  ACCAAAAT TATCACAATATAA 7
    loxF9b CCATCTTTTGTTA  GATTTGAA TATATACATTCTA 8
  • These sequences are present in the human genome and flank exon 8 of the F9 gene. The invention comprises a nucleic acid selected from SEQ ID NO. 7 (loxF9a) and SEQ ID NO. 8 (loxF9b) or a sequence with a least 90% sequence identity, preferably a least 95 sequence identity, more preferably at least 99% sequence identity, to SEQ ID NO. 7 or SEQ ID NO. 8 or a nucleic acid sequence reverse complementary to one of these sequences and their use as target site for a DNA recombining enzyme, preferably a F9 designer DNA recombining enzyme according to the invention. In one embodiment these nucleic acids according to the invention have a length of 30 to 40 nucleotides, preferably 32 to 36, more preferably 34 nucleotides.
  • The invention also comprises nucleic acids and vectors encoding for the F9 designer DNA-recombining enzyme according to the invention.
  • The invention also comprises a method for transduction of a cell with:
      • 1. a delivery vector comprising a nucleic acid encoding for the F9 designer DNA-recombining enzyme according to the invention,
      • 2. a donor sequence that comprises the loxF9a and LoxF9b sites surrounding a sequence encoding for the exon 8 of the F9 gene or a functional mutant thereof, and expression of the DNA-recombining enzyme.
  • The donor sequence can be located on the expression or delivery vector or is provided as separate nucleic acid, preferably a replication deficient plasmid. Preferably, the donor sequence is provided at multiple copies per cell to increase the likelihood of replacement of the target sequence (see also FIG. 3 and exemplified in FIG. 11 ).
  • The invention further comprises a method for replacing a (non-or malfunctional) mutant exon 8 of the F9 gene in a cell, by introducing into a cell:
      • a F9 designer DNA-recombining enzyme according to the invention or a nucleic acid or vector encoding and expressing it,
      • a donor sequence comprising a sequence encoding for the exon 8 of the F9 gene or a functional mutant thereof flanked by the loxF9a and loxF9b target sites,
      • whereas the F9 designer DNA-recombining enzyme replaces a sequence comprising the mutant exon 8 by the donor sequence by site specific recombination.
  • The invention also comprises a kit comprising
      • a F9 designer DNA-recombining enzyme according to the invention or a nucleic acid or vector encoding it,
      • a donor sequence comprising a sequence encoding for the exon 8 of the F9 gene or a functional mutant thereof flanked by the loxF9a and loxF9b target sites.
  • In a preferred embodiment, the defective F9 exon 8 sequence is replaced by a donor sequence carrying the hyperactive Padua mutation (R338L) (PMID: 23197580).
  • Particular preferred donor sequences for the above mentioned methods and the kit are selected from SEQ ID No. 26 (wild type exon 8) and SEQ ID No. 27 (exon 8 with Padua mutation)
  • The donor sequence can be located on the vector encoding for the F9 designer DNA-recombing enzyme (s. FIG. 11 for an example) or is provided as separate nucleic acid, preferably a replication deficient plasmid.
  • Preferably, the donor sequence is provided in the above-mentioned methods and the kit at multiple copies per cell (e.g. on an AAV vector) to increase the likelihood of replacement of the target sequence (see also FIG. 11 for an example)).
    • EXAMPLE HEX
  • In a preferred embodiment for DRiGD the designer DNA-recombining enzyme according to the invention deletes a sequence on the human chromosome 7. This designer DNA-recombining enzyme is further referred to as Hex designer DNA-recombining enzyme and recognizes the asymmetric Hex1 and Hex2 target sites according SEQ ID No 40 and SEQ ID No 41:
  • SEQ ID
    Name Sequence No.
    Hex1 TACACAGTGTATATTGATTTTTATCAAATGCCTT 40
    Hex2 TACACAATGTATATTGATTTTTATCAAATGCCTT 41
  • In one embodiment, the designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 46 (Hex-R-#7) or a sequence with a least 90% sequence identity, more preferably 95% sequence identity to SEQ ID NO. 46 and a monomer with the sequence according to SEQ ID NO. 47 (Hex-L-#7) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 47.
  • In another embodiment, the designer DNA-recombining enzyme is a heterotetramer comprising two different monomers: a monomer with the sequence according to SEQ ID NO. 48 (Hex-R-#30) or a sequence with a least 90% sequence identity, more preferably 95 sequence identity to SEQ ID NO. 48 and a monomer with the sequence according to SEQ ID NO. 49 (Hex-L-#30) or a sequence with a least 80% sequence identity, preferably 90% sequence identity, more preferably 95% or even 98% sequence identity to SEQ ID NO. 49.
  • The invention also comprises nucleic acids and vectors encoding for the Hex designer DNA-recombining enzyme according to the invention.
  • In a further aspect the invention includes the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention for use in medicine, in particular for the treatment of a disease or condition associated with one of the above-mentioned disease-associated genes or polynucleotides or the above mentioned signaling biochemical pathway-associated genes and polynucleotides.
  • In a similar aspect the invention includes the preparation of a medicament for the treatment of a disease or condition associated with one of the above-mentioned disease genes or polynucleotides or the above mentioned signaling biochemical pathway-associated genes and polynucleotides comprising the DNA recombining enzymes according to the invention, the nucleic acids or vectors according to the invention or the set/kit according to the invention or using a method according the invention.
  • It is noted that in this disclosure and particularly in the claims and/or paragraphs, terms such as “comprises”, “comprised”, “comprising” and the like can have the meaning attributed to it in U.S. Patent law; e.g., they can mean “includes”, “included”, “including”, and the like; and that terms such as “consisting essentially of” and “consists essentially of” have the meaning ascribed to them in U.S. Patent law, e.g., they allow for elements not explicitly recited, but exclude elements that are found in the prior art or that affect a basic or novel characteristic of the invention. These and other embodiments are disclosed or are obvious from and encompassed by, the following Detailed Description.
  • Further the embodiments described in the invention may be combined with each other.
  • The invention is illustrated by the following figures and non-limiting examples:
  • FIG. 1 shows a general scheme of a preferred Designer DNA Recombining enzyme induced Gene Replacement (DRiGR) according to the invention. Different monomers R1-4 of DNA recombining enzymes and their respective DNA binding sequences loxR1-loxR4 (half sites of target sites) are shown. The site of interest in the genome (frowny face) is replaced by a desired sequence (smiley).
  • FIG. 2 shows a general scheme of a Designer DNA Recombining enzyme induced Gene Deletion (DRiGD). Again, different monomers R1-4 of DNA recombining enzymes binding at their respective DNA binding sequences loxR1-loxR4 are shown in the upper scheme. Here the target sites bear each identical 8 bp spacers. The site of interest in the genome (frowny face) is here cut out.
  • FIG. 3 illustrates that if the donor vector is in excess to the genomic DNA, Gene Replacement induced by the DNA recombining enzyme is driven towards the replacement of the genomic DNA with the donor vector DNA. Preferably, the donor vector is present in multiple copies in the nucleus of the target cell, such as in AAV vectors, which results in efficient repair of cellular mutations.
  • FIG. 4 demonstrates a general method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (Duo-SLIDE DRiGR) that delivers a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with two libraries of DNA recombining enzymes. Expression of single recombinases in this system is also feasible (s. FIG. 15 ). R1, R2 and R3 stand for three different restriction enzymes or the respective restriction sites. P1 and P2 stand for two different PCR primers or the respective primer binding sites.
  • When these monomers of DNA recombining enzymes are expressed in conjunction with a DNA template also carrying the two target sites (here the pDonor plasmid), recombination at both target sites leads to the exchange of the DNA fragments. Because the incoming DNA fragment removes a restriction site from the pDuoSLiDE vector, effective recombinases that promoted the replacement can be amplified by PCR after digestion of the DNA with the restriction enzyme. Cycling through this system in combination with random mutagenesis and DNA shuffling uncovers the most efficient recombinases promoting the reaction. By using an origin of replication (R6K-ori) on pDonor that is inactive in the host, the process of integration through one target site (e.g. lox1) and excision through the other target site (e.g. lox2) can be uncoupled. Furthermore, integration can be identified through antibiotic selection on kanamycin containing plates.
  • FIG. 5 demonstrates a similar method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (QuSLiDE DRiGR) that delivers two pairs of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites. Here each monomer is directed to one half site of one target site (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with four libraries of DNA recombining enzymes. (R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .)
  • If the DNA on the donor vector contains the wild-type allele and the genome contains a disease causing mutation, the gene defect is repaired in cells (s. FIG. 1 ).
  • FIG. 6 demonstrates a general method to obtain a DNA Recombining enzyme for DRiGD by applying a novel directed evolution strategy (Duo-SLIDE DRiGD) that delivers a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with two libraries of DNA recombining enzymes. (R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .)
  • When these monomers of DNA recombining enzymes are expressed, recombination between two copies of the pDuoSLiDE vector removes a restriction site from the pDuoSLiDE vector, effective recombinases that promoted the deletion can be amplified by PCR after digestion of the DNA with the restriction enzyme. Cycling through this system in combination with random mutagenesis and DNA shuffling uncovers the most efficient recombinases promoting the reaction.
  • FIG. 7 demonstrates a similar method to obtain a DNA Recombining enzyme for DRiGD by applying a novel directed evolution strategy (QuSLiDE DRiGD)) that delivers two pairs of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites. Here each monomer is directed to one half site of one target site (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with four libraries of DNA recombining enzymes. (R1, R2 and R3 as well as P1 and P2 have the meaning as in FIG. 4 .)
  • This set of monomers allows deleting a site of interest in a Genome (s. FIG. 2 ).
  • FIG. 8 shows a method know in the state of the art to obtain a tailored recombinase that works one target sites by substrate-linked protein evolution (SLiPE) (Buchholz F and Stewart AF, 2001). Here the vector only contains one library and two identical target sites (dashed triangles).
  • FIGS. 9A-9C show the application of the invention to replace a mutation in the exon 8 of the F9 gene by Duo-SLIDE DRiGR. FIG. 9A shows the schematic presentation of exon 8 and flanking sequence of the F9 gene. The two selected target sequences (loxF9a—SEQ ID No. 3 and loxF9b—SEQ ID No. 4) are indicated by triangles (black and white) and the nucleotide sequence and the chromosomal positions are shown. FIG. 9B illustrates the method to obtain the two recombinase monomers F9-1 (SEQ ID No. 1, 3, 5 and 28) and F9-2 (SEQ ID No. 2, 4, 6 and 29) and the replacement reaction. Confirmation that the recombination reactions have happened as predicted was confirmed by DNA sequencing. FIG. 9C shows an agarose gel demonstrating the restriction pattern for pDuoF9 (source) (lane 1) and pDuoF9 (product) (lane 2) after digestion with NdeI and SacI. M=molecular marker.
  • FIG. 10 shows an exemplary evolution process of adapting the libraries of DNA recombining enzymes to a given target site—here applied to the loxF9a and loxF9b target sites.
  • Here DNA recombining enzymes that are active on the loxF9a site (F9A) were obtained by designing two symmetric intermediate sites AL and AR and performing 20 or 17 cycles of SLiPE (s. FIG. 8 ). After combining and shuffling the libraries of DNA recombining enzymes obtained 108 cycles of SLiPE were performed on the asymmetric loxF9a site (F9A). Agarose gels of indicated generation cycles are shown, with the line with two triangles indicating the non-recombined band and the line with one triangle showing the size of the recombined band. The grey triangle below the gel pictures indicates the reduced amount of L-arabinose added to the growth medium. To obtain DNA recombining enzymes that are active on the loxF9b site (F9B) a first set of two symmetric intermediate sites BLS and BRS were designed and 9 cycles of SLiPE (s. FIG. 8 ) were performed. After shuffling the libraries of DNA recombining enzymes again SLiPE was performed on a second set of symmetric intermediate sites BL (30 cycles) and BR (22 cycles) was performed. Finally, after combing and shuffling the libraries of DNA recombining enzymes obtained 17 cycles of SLIPE were performed on the asymmetric loxF9b site (F9B). Finally (not shown) the two libraries of DNA recombining enzymes obtained on the loxF9a site and the loxF9b site were cloned into pDuoF9 (source)—s. FIGS. 9A-9C.
  • FIG. 11 illustrates the use the F9 designer DNA recombining enzymes in a therapeutic setting, both F9-1 and F9-2 coding sequences are cloned into a delivery vector (such as an adeno-associated viral vector) together with the donor sequence that contains loxF9a and loxF9b flanking the wild-type, or Padua mutation (R338L) of exon 8 of the F9 gene. Delivery of such a vector into target cells in multiple copies replaces the inactivating mutation in the genome.
  • FIG. 12 shows the result of a screen of the human PCSK9 Gene on Chromosome 1 for 8 bp repeats. The exons of the PCSK9 Gene are indicated by bold boxes. The 8 bp repeats are separated by at least 150 bp and no more than 2 kb apart and lie in potential target sites (indicated by vertical lines) with a length of 34 bp, that were screened not occurring elsewhere in the genome. The sequences of interests between the potential target sites are indicated by horizontal lines.
  • FIG. 13 shows the result of a similar screen of the genome of the human papilloma virus 16 (HPV16) for 8 bp repeats that are separated by at least 150 bp and no more than 2 kb apart and lie in potential target sites of 34 bp, that were screened not to occur in the human genome. The sequences encoding for the viral proteins are indicated in bold boxes with arrows (greater-than signs).
  • FIG. 14 demonstrates a different general method to obtain a DNA Recombining enzyme for DRiGR used in example 3 step 3 by applying a novel directed evolution strategy (SLIDE DRiGR 2.0) that delivers a library of DNA recombining enzymes that recombines two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting. R1, R2, R3, R4 and R5 stand for five different restriction enzymes or the respective restriction sites. P3 and P4 stand for two different PCR primers or the respective primer binding sites. When this library of DNA recombining enzymes is expressed in the presence of a DNA template also carrying the two target sites (here the pDonor-ex8 plasmid), recombination at both target sites leads to the exchange of the DNA fragments. Because the incoming DNA fragment removes restriction sites from the pF9 vector, effective recombinases that promoted the replacement can be amplified by PCR after digestion of the DNA with the restriction enzymes R1-3 and RecBCD. Cycling through this system in combination with random mutagenesis and DNA shuffling uncovers the most efficient recombinases promoting the DRIGR reaction.
  • FIG. 15 highlights the steps of the novel directed evolution strategy (SLIDE DRiGR) used in example 3 step 3. In a sub step 1, pF9 (source) and pDonor are transformed into bacteria. Only if the expression of the recombinases is induced, growth of colonies resistant to the antibiotic can be observed. In sub step 2, the plasmid DNA of the obtained colonies is digested using restriction enzymes R1-3. This will enrich for clones which have undergone successful DRIGR and result in a pure preparation of pF9 (product). The purity of the plasmid preparation is verified with a restriction digest using the enzymes XhoI and XmaI, which shows the unique restriction pattern of pDuoF9 (product). Additionally, DNA sequencing reveals the correct sequence of pF9 (product).
  • FIG. 16 demonstrates a different general method to obtain a DNA Recombining enzyme for DRiGR by applying a novel directed evolution strategy (Duo-SLIDE DRiGR 2.0) that delivers one or a pair of monomers of DNA recombining enzymes that work in conjunction to recombine two different target sites (white triangle lox 1 and black triangle lox 2) present in the genome starting with a source vector with one or two libraries of DNA recombining enzymes. R1, R2, R3, R4 and R5 stand for five different restriction enzymes or the respective restriction sites. P3 and P4 stand for two different PCR primers or the respective primer binding sites. When these monomers of DNA recombining enzymes are expressed in conjunction with a DNA template also carrying the two target sites (here the high-copy number plasmid pDonor-ex8), recombination at both target sites leads to the exchange of the DNA fragments. Because the incoming DNA fragment removes a restriction site from the pDuoSLiDE vector, effective recombinases that promoted the replacement can be amplified by PCR after digestion of the DNA with the restriction enzyme. Importantly, no antibiotic selection marker is used in this assay. Cycling through this system in combination with random mutagenesis and DNA shuffling uncovers the most efficient recombinases promoting the reaction.
  • FIG. 17 shows alignment between Cre (state of the art), a recombinase obtained after step 1 of example 3 (R #1), a recombinase obtained after step 2 of example (3R #7-B5), and a recombinase obtained after step 3 of example 3 (F9-3). The alignment shows how additional mutations have arisen during later stages of evolution.
  • FIG. 18 shows a general scheme of the method of the invention, exemplified by the DRiGD method and steps of the invention.
  • FIG. 19 and FIG. 20 show a more detailed scheme of the method of the invention, exemplified by the DRiGD method and applied to the human genome.
  • FIGS. 21A-21C illustrate the DRIGD method of the invention of applied to a target site on the human chromosome 7—further described in example 5. FIG. 21A shows the target sites: Hex 1 and Hex 2 are the target sites used for excision—HexL, HexR, HexR1 and HexR2 are intermediate target sites. FIG. 21B shows the evolution of specific recombinases for the Hex1 and Hex2 target sites. FIG. 21C shows recombination efficiency of two clones (Clone #7 and Clone #30) obtained.
  • FIG. 22 shows the precise excision of a sequence from a plasmid substrate using the recombinases obtained in example 5.
    •  EXAMPLE 1
  • To demonstrate the utility of DRiGD, the inventors have screened the human genome for all 8 bp repeats, separated by at least 150 bp: The potential target sites are indicated sequences sequences 13 bp left and 13 bp right of the 8 bp repeat not occurring elsewhere in the human genome in a window to delete a maximum of 2 kb from the genome. FIG. 12 shows the results for all 309 possible target sites for DNA recombining enzymes in the human gene PCSK9. FIG. 13 shows the results for all 176 possible target sites for DNA recombining enzymes in the human papilloma virus 16 (HPV16).
    • EXAMPLE 2
  • To demonstrate the feasibility of the DRiGR invention, the inventors have generated recombinases that when applied in concert can replace exon eight of the human factor 9 (F9) gene. This exon frequently carries mutations in patients suffering from hemophilia B. Our results demonstrate that this exon can be efficiently replaced for a different sequence providing an appropriate donor vector and expression of two recombinases working in concert (FIG. 6 , FIGS. 9A-9C and FIG. 11 ).
  • Materials and methods as described in WO 2008/083931 A1, WO 2011/147590 A1, WO2016034553 A1 and in the publication Buchholz F and Stewart AF, 2001 are used, if not specified otherwise.
  • The target sequences (loxF9a—SEQ ID No. 7 and loxF9b—SEQ ID No. 8) were selected by comparing sequences in the human genome with half sites of previously identified target sites (LoxP, LoxH, LoxM7, LoxM5, loxLTR, LoxBTR) of DNA recombining enzymes:
  • Name Sequence SEQ ID No.
    loxF9a CTCATTACATTTA  ACCAAAAT TATCACAATATAA  7
    loxF9b CCATCTTTTGTTA  GATTTGAA TATATACATTCTA  8
    loxP ATAACTTCGTATA  ATGTATGC TATACGAAGTTAT  9
    loxH ATATATACGTATA  TAGACATA TATACGTATATAT 10
    loxM7 ATAACTCTATATA ATGTATGC TATATAGAGTTAT 11
    LoxM5 ATAACTTCGTGCA ATGTATGC TGCACGAAGTTAT 12
    loxLTR ACAACATCCTATT  ACACCCTA TATGCCAACATGG 13
    loxBTR AACCCACTGCTTA AGCCTCAA TAAAGCTTGCCTT 14
  • In the table the first (left) and second (right) half sites in the target sites are underlined.
  • In a first step the two recombinase libraries were evolved separately using the SLIDE approach as described before (Buchholz and Stewart, 2001 and WO 2002044409 A2—s. FIG. 8 ). In short, each library was evolved towards recombining intermediate target sites (AL, AR, BLS, BLRS, BL, BR) and finally to recombining one of the two target sites, loxF9a or loxF9b. New mutations were introduced through error-prone PCR and DNA-shuffling and selective pressure was further regulated via the expression level of the recombinases. Active recombinases were selected by their ability to recombine the respective target site to delete the sequence flanked by the target sites from the vector (s. FIG. 8 ).
  • The following intermediate sequences were used:
  • Name Sequence SEQ ID No.
    loxF9-AL CTCATTACATTTA ACCAAAAT TAAATGTAATGAG 15
    loxF9-AR TTATATTGTGATA ACCAAAAT TATCACAATATAA 16
    loxF9-BLS CCAACTTTTGATA  GATTTGAA TATCAAAAGTTGG 17
    loxF9-BRS TAGACTTTATATA  GATTTGAA TATATAAAGTCTA 18
    loxF9-BL CCATCTTTTGTTA GATTTGAA TAACAAAAGATGG 19
    LOXF9-BR TAGAATGTATATA  GATTTGAA TATATACATTCTA 20
  • Recombinase coding sequences from these two libraries were then cloned in the vector backbone pDuoF9 (source) (SEQ ID No. 21, s. FIGS. 9A-9C). In this vector the two recombinase libraries are expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together. The size of this library exceeded 100.000 clones.
  • To carry out DRiGR, 50 μl of electrocompetent E. coli XL1-Blue cells were transformed with 1 ng pDuoF9 (source) library and grown overnight in 200 ml LB medium containing 15 μg/ml chloramphenicol. On the next day, 1 ml of the overnight culture was used to inoculate 100 ml of fresh medium and grown for 2 h. Then the culture was split into 2×50 ml and L-arabinose was added to a final concentration of 50 μg/ml to induce recombinase expression in one of the cultures. After 2.5 h of incubation the cells were put on ice and prepared for electroporation as described before (Sambrook and Russell, 2001). The electrocompetent cells were resuspended in 200 μl water, and 50 μl of the cell suspension was immediately used for transformation of 0.4 ng pDonor at 1700 V. The bacteria were then allowed to recover for 2 h in SOC medium before the entire suspension was plated on LB agar plates containing 15 μg/ml kanamycin.
  • No colonies had grown overnight on the agar plates where uninduced cells had been plated, validating that recombinase expression was required for the integration of the pDonor vector (SEQ ID No. 22). All colonies growing from the induced samples were pooled and cultured in 220 ml LB medium containing 15 μg/ml kanamycin and 50 μg/ml L-arabinose to allow possible recombination through the second pair of target sites.
  • On the next day the plasmid DNA was isolated from the culture. To enrich for clones that had undergone successful DRiGR, the DNA was digested with enzymes cleaving any non-pDuoF9 (product)—negative selection—and re-transformed. To this end, 500 ng of plasmid preparation were digested with 1 μl of each NdeI, AvrII and PspXI. After 3 h incubation at 37° C. DNA was precipitated and resuspended in 50 μl water. 1 μl of this was used for transformation of 50 μl electrocompetent E. coli XL1-Blue cells. The transformed cells were grown in 220 ml LB medium containing 15 μg/ml kanamycin—positive selection-.
  • On the next day the plasmid DNA was isolated from the culture. The recombinase libraries were amplified by PCR using primers P1 and P2 (SEQ ID No.23 and 24) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI. The isolated recombinase libraries were then ligated back into pDuoF9 (source) to start another cycle of DRiGR. Three rounds of Duo-SLIDE DRIGR (s. FIG. 10 ) were performed to enrich for recombinases that can efficiently carry out DRiGR on the target sites loxF9a and LoxF9b.
  • Primer Used:
  • Name Sequence SEQ ID No.
    P1 CTCTACTGTTTCTCCATAC 23
    P2 AGGGAATAAGGGCGACA 24
  • The sequences of the plasmid pDuoF9 (product) is given in SEQ ID No.25
  • By this method the sequences of the following pairs of recombinase monomers were obtained:
      • Rec F9-1a: SEQ ID No. 3 and Rec F9-2a: SEQ ID No. 4
      • Rec F9-1b: SEQ ID No. 5 and Rec F9-2b: SEQ ID No. 6
    EXAMPLE 3
  • Step 1:
  • In a first step the two recombinase libraries were evolved separately using the SLIDE approach as described before (Buchholz and Stewart, 2001 and WO 2002044409 A2—s. FIG. 8 ). In short, each library was evolved as described in example 2 towards recombining intermediate target sites (loxF9-AL, AR, BLS, BLRS, BL, BR—s. example 2) and finally to recombining one of the two target sites, loxF9a or loxF9b. New mutations were introduced through error-prone PCR and DNA-shuffling and selective pressure was further regulated via the expression level of the recombinases. Active recombinases were selected by their ability to recombine the respective target site to delete the sequence flanked by the target sites from the vector (s. FIG. 8 ).
  • Step 2:
  • In a second step a single recombinase library was evolved to carry out DRiGR following a selection scheme with the replication deficient pDonor (s. FIGS. 9A-9C). This involved cloning the recombinase coding sequences from the libraries generated in step 1 in the vector backbone pF9 (source) (=pDuoF9 SEQ ID No. 21). In this vector one to four recombinase libraries can be expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together. The size of this library exceeded 100.000 clones.
  • To carry out DRiGR, 50 μl of electrocompetent E. coli XL1-Blue cells were transformed with 1 ng pDuoF9 (source) library and grown overnight in 200 ml LB medium containing 15 μg/ml chloramphenicol. On the next day, 1 ml of the overnight culture was used to inoculate 100 ml of fresh medium and grown for 2 h. Then the culture was split into 2×50 ml and L-arabinose was added to a final concentration between 1-200 μg/ml to induce recombinase expression in one of the cultures. After 2.5 h of incubation the cells were put on ice and prepared for electroporation as described before (Sambrook and Russell, 2001). The electrocompetent cells were resuspended in 200 μl water, and 200 μl of the cell suspension was immediately used for transformation of 80 ng pDonor at 1700 V. The bacteria were then allowed to recover for 2 h in SOC medium before the entire suspension was used to incoculate 200 ml LB medium containing 15 μg/ml chloramphenicol, 5 μg/ml kanamycin and 1-200 μg/ml L-arabinose. A small part of the suspension was plated on agar plates to estimate the library size.
  • On the next day it was verified that no colonies had grown on the agar plates where uninduced cells had been plated, validating that recombinase expression was required for the integration of the pDonor vector (SEQ ID No. 22). The plasmid DNA was isolated from the liquid culture. To enrich for clones that had undergone successful DRiGR, the DNA was digested with enzymes cleaving any non-pDuoF9 (product)—negative selection—and re-transformed. To this end, 500 ng of plasmid preparation were digested with 1 μl of each NdeI, AvrII, PspXI and FspI. After 3 h incubation at 37 t the DNA was cleaned-up via microdialysis on a membrane filter. 3 μl of this digest was used for transformation of 50 μl electrocompetent E. coli XL1-Blue cells. The transformed cells were grown in 100 ml LB medium containing 15 μg/ml chloramphenicol and 15 μg/ml kanamycin—positive selection-.
  • On the next day the plasmid DNA was isolated from the culture, and 500 ng of DNA was digested with 1 μl of each NdeI and AvrII. The recombinase libraries were amplified by error-prone PCR using primers P1 # and P2 #(SEQ ID No. 30 and 31) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI. The isolated recombinase libraries were then ligated back into pDuoF9 (source) to start another cycle of DRiGR. After cycles 3 and 8 DNA shuffling was carried out as described before (Buchholz and Stewart, 2001). Eleven rounds of Duo-SLIDE DRIGR were performed to enrich for recombinases that can efficiently carry out DRiGR on the target sites loxF9a and loxF9b.
  • Step 3:
  • In a third step a single recombinase library was evolved to carry out DRiGR 2.0 following a different selection scheme (s. FIG. 14 ) with the high-copy number plasmid pDonor-ex8 (s. FIG. 14 , SEQ ID 37) without antibiotic selection and without separating integration from resolution of the donor vector. This involved cloning the recombinase coding sequences from the library generated in step 2 in the vector backbone pF9 (source) (=pDuoF9 (source) SEQ ID No. 21). The library was then transformed into electrocompetent E. coli XL1-Blue cells which were already carrying pDonor-ex8. The transformed cells were grown in 100 ml LB medium containing 15 μg/ml chloramphenicol, 5 μg/ml kanamycin and 1-200 μg/ml L-arabinose. The size of this library exceeded 100.000 clones.
  • On the next day the plasmid DNA was isolated from the culture, and 1000 ng of DNA was digested with 1 μl of each NdeI, AwlI, PspXI (restriction enzymes R1, R2 and R3—s. FIGS. 14 and 15 ) and the exonuclease RecBCD. The recombinase libraries were amplified by error-prone PCR using primers P3 and P4 (SEQ ID No. 32 and 33) and the PCR products were purified on a column and subjected to a digest with the restriction enzymes SacI and SbfI. The isolated recombinase libraries were then ligated back into pF9 (source) and transformed into electrocompetent E. coli XL1-Blue cells carrying pDonor-ex8. The transformed cells were grown in 100 ml LB medium containing 15 μg/ml chloramphenicol, 5 μg/ml kanamycin and 1-200 μg/ml L-arabinose and the next cycle thus began. Every three cycles DNA shuffling was carried out as described before (Buchholz and Stewart, 2001). Eleven rounds of SLIDE DRIGR (s. FIG. 14 ) were performed to enrich for recombinases that can efficiently carry out DRIGR on the target sites loxF9a and LoxF9b without antibiotic selection and without separating integration from resolution of the donor vector.
  • Primers Used:
  • Name Sequence SEQ ID No.
    P1# CGGCGTCACACTTTGCTATG 30
    P2# CCCTTAAACGCCTGGTGCTA 31
    P3 AAGATTAGCGGATCCTACCT 32
    P4 GTGATTAGTTAGTGAGAGGC 33
  • By this method the sequences of the following recombinase monomers were obtained: R#1 (SEQ ID No. 34) after step 1: R#7-B5 (SEQ ID No. 35) after step 2 and Rec F9-3 (SEQ ID No. 36) after the final step 3.
  • The recombinase efficiency (DRGR efficiency) of the recombinases obtained after step 1, 2 and 3 are compared in the following table:
  • Example
    Selection Recombinase DRIGR
    Step scheme obtained SEQ ID No. efficiency
    1 SLIDE R# 1 34  0.5%
    (s. FIG. 8)
    2 Duo-SLIDE R#7-B5: 35 12.3%
    DRIGR
    (s. FIG. 9A-9C)
    3 SLIDE-DIGR F9-3 36  90%
    2.0 (s. FIG. 14)
    • EXAMPLE 4
  • To utilize the F9 recombinases in a therapeutic setting, both F9-1 and F9-2 coding sequences are cloned into a delivery vector (such as an adeno-associated viral vector) together with the donor sequence that contains loxF9a and loxF9a flanking the wild-type, or Padua mutation (R338L) of exon 8 of the F9 gene. Delivery of such a vector into target cells in multiple copies replaces the inactivating mutation in the genome (s. FIG. 11 ).
    • EXAMPLE 5
  • To demonstrate the utility of DRiGD recombinases for the excision of a specific DNA sequence on chromosome 7 of the human genome were evolved.
  • First, two different recombinases libraries were evolved using the described protocol of substrate linked directed evolution (Buchholz and Stewart, 2001 and WO 2002044409 A2—s. FIG. 8 ). The libraries were evolved against the left or the right half-site (HexL or HexR) of the final target sites Hex1 and Hex2 (s. FIG. 21A). To evolve the recombinase libraries for HexR, two intermediated target sites (HexR1 and HexR2) were used (s. FIG. 21B). Throughout the evolution process new mutations were introduced with error-prone PCR (using Primers P1 # and P2 #) and DNA-shuffling and selective pressure was further regulated via the expression level of the recombinases. Active recombinases were selected by their ability to recombine the respective target site to delete the sequence flanked by the target sites from the vector (s. FIG. 8 —Standard SLiPE and FIG. 21B).
  • Next, both libraries (obtained on HexR and HexL) were combined in order to recombine the final target sites Hex1 and Hex2. Therefore, the libraries (library size was bigger than 100.000 recombinases) were cloned into the same expression plasmid and were co-expressed from a shared inducible promoter in an operon-like structure, which transcriptionally links both enzymes together (s. FIG. 6 pDUO-SLiDE DRiGD). The plasmids carrying both libraries were then transformed into XL-1 blue E. coli cells. After recovering the cells in 1 ml of SOC medium for one hour, 10 μl was plated on a LB agar plate with 15 μg/ml chloramphenicol. The next day 32 clones were picked and cultured in 500 μl of LB with 25 μg/ml chloramphenicol for 8 h. Next, 250 μl pre-culture was used to inoculate 2×5 ml of LB with 25 μg/ml chloramphenicol. In one of the 5 ml cultures 10 μg/ml L-arabinose was added to induce the expression of the recombinase dimer. The next day plasmid DNA was isolated and digested with SacI (R2) and SbfI (R3) to estimate the recombination efficiency in the induced and uninduced sample.
  • With this method two recombinase dimers (Clone #7 and Clone #30) were obtained that can carry out the excision reaction at high efficiency. The precise excision was confirmed by sequencing the recombined pDUO-SLiDE DRiGD—s. FIG. 22 .
  • The target sites and the intermediate target sites and primers used are listed in the following table:
  • Hex
    target sites SEQ-ID
    Hex1 TACACAGTGTATATTGATTTTTATCAAATGCCTT 40
    Hex2 TACACAATGTATATTGATTTTTATCAAATGCCTT 41
    HexL TACACAGTGTATATTGATTTTTATACATTGTGTA 42
    HexR AAGGCATTTGATATTGATTTTTATCAAATGCCTT 43
    HexR1 AAGACATTTTATATTGATTTTTATAAAATGTCTT 44
    HexR2 AACGCATTGGATATTGATTTTTATCCAATGCGTT 45
    P1# CGGCGTCACACTTTGCTATG 30
    P2# AAGGGAATAAGGGCGACACG 31
  • By this method the sequences of the following pairs of recombinase monomers were obtained:
      • Hex-R-#7: SEQ ID No. 46 and Hex-L-#7: SEQ ID No. 47
      • Hex-R-#30: SEQ ID No. 48 and Hex-L-#30: SEQ ID No. 49
    CITED NON-PATENT LITERATURE
    • Buchholz, F., & Stewart, A. F. (2001). Alteration of Cre recombinase site specificity by substrate-linked protein evolution. Nature Biotechnology, 19(11), 1047-1052.
    • Karpinski J, Hauber I, Chemnitz J, et al. (2016). Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nat Biotechnol 34(4):401-9.
    • Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: A Laboratory Manual, (3rd edition).
    • Surendranath, V. et al. (2010). SeLOX—a locus of recombination site search tool for the detection and directed evolution of site-specific recombination systems. Nucleic Acids Res. 2010, 38(W293-W298)
    • Wang M et al. (2016). Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles. PNAS 113:2868-2873.
    • Zhang, C et al. (2015) Redesign of the monomer—monomer interface of Cre recombinase yields an obligate heterotetrameric complex. Nucleic Acids Res. 2015, 43 (18): 9076-9085.

Claims (21)

1-26. (canceled)
27. A method for site-specific DNA recombination, comprising introducing into a host cell a designer DNA-recombining enzyme, wherein the designer DNA-recombining enzyme comprises at least two different monomers, wherein the at least two different monomers recognize non-identical naturally occurring half sites in a genome of a host cell.
28. The method of claim 27, wherein the recombination causes a deletion, an insertion, an inversion, or a replacement of genetic content.
29. The method of claim 27, wherein the genome is a human genome.
30. The method of claim 27, wherein the enzyme is capable of inducing a site-specific DNA recombination in a genome of a host cell by recombining two target sequences naturally occurring in the genome.
31. The method of claim 27, wherein the target sequences are not identical or wherein the target sequences are identical.
32. The method of claim 27, wherein the designer DNA-recombining enzyme is capable of causing a deletion, an insertion, an inversion, or a replacement of a nucleotide sequence in the genome of the host cell.
33. The method of claim 27, wherein each target sequence comprises two half sites with an intervening spacer sequence.
34. The method of claim 27, wherein the half sites are between 10-20 nucleotides in length.
35. The method of claim 27, wherein the half sites are 13 nucleotides in length.
36. The method of claim 27, wherein the half sites differ from each other by not more than 7 nucleotides.
37. The method of claim 27, wherein the half sites differ from each other by not more than 5 nucleotides.
38. The method of claim 27, wherein the half sites differ from each other by not more than 4 nucleotides.
39. The method of claim 27, wherein the half sites differ in at least two nucleotides, at least three nucleotides, at least four nucleotides, or at least eight nucleotides.
40. The method of claim 33, wherein the spacer sequence is between 5 and 12 nucleotides in length.
41. The method of claim 33, wherein the spacer sequence is 8 nucleotides in length.
42. The method of claim 33, wherein the spacer sequence comprises a non-palindromic sequence having at least 2 positions of asymmetry.
43. The method of claim 33, wherein the spacer sequence comprises a non-palindromic sequence having at least 4 positions of asymmetry.
44. The method of claim 33, wherein the spacer sequence comprises a non-palindromic sequence having at least 6 positions of asymmetry.
45. The method of claim 33, wherein each target sequence comprises an identical spacer sequence.
46. The method of claim 33, wherein each target sequence comprises a non-identical spacer sequence.
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