JP2019515666A5 - - Google Patents
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- Publication number
- JP2019515666A5 JP2019515666A5 JP2018552794A JP2018552794A JP2019515666A5 JP 2019515666 A5 JP2019515666 A5 JP 2019515666A5 JP 2018552794 A JP2018552794 A JP 2018552794A JP 2018552794 A JP2018552794 A JP 2018552794A JP 2019515666 A5 JP2019515666 A5 JP 2019515666A5
- Authority
- JP
- Japan
- Prior art keywords
- rrs
- vector
- nucleotide sequence
- sequence encoding
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 239000013598 vector Substances 0.000 claims description 226
- 239000002773 nucleotide Substances 0.000 claims description 123
- 125000003729 nucleotide group Chemical group 0.000 claims description 123
- 108020004707 nucleic acids Proteins 0.000 claims description 85
- 102000039446 nucleic acids Human genes 0.000 claims description 85
- 150000007523 nucleic acids Chemical class 0.000 claims description 85
- 210000004027 cell Anatomy 0.000 claims description 71
- 102100030355 Host cell factor 1 Human genes 0.000 claims description 62
- 239000003550 marker Substances 0.000 claims description 59
- 108090000623 proteins and genes Proteins 0.000 claims description 55
- 102000025171 antigen binding proteins Human genes 0.000 claims description 29
- 108091000831 antigen binding proteins Proteins 0.000 claims description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 18
- 208000005229 Autosomal recessive Robinow syndrome Diseases 0.000 claims description 16
- 230000002708 enhancing effect Effects 0.000 claims description 16
- 238000001945 resonance Rayleigh scattering spectroscopy Methods 0.000 claims description 16
- 102000018120 Recombinases Human genes 0.000 claims description 8
- 108010091086 Recombinases Proteins 0.000 claims description 8
- 108020004705 Codon Proteins 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 150000001413 amino acids Chemical class 0.000 claims description 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 4
- 108700026220 vif Genes Proteins 0.000 claims description 4
- 230000001404 mediated effect Effects 0.000 claims description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims description 2
- 230000006798 recombination Effects 0.000 claims description 2
- 238000001890 transfection Methods 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- 230000010354 integration Effects 0.000 description 2
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021114374A JP2021164479A (ja) | 2016-04-20 | 2021-07-09 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2023201924A JP7781128B2 (ja) | 2016-04-20 | 2023-11-29 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2025170326A JP2025188169A (ja) | 2016-04-20 | 2025-10-08 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662325400P | 2016-04-20 | 2016-04-20 | |
| US62/325,400 | 2016-04-20 | ||
| PCT/US2017/028555 WO2017184832A1 (en) | 2016-04-20 | 2017-04-20 | Compositions and methods for making antibodies based on use of expression-enhancing loci |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021114374A Division JP2021164479A (ja) | 2016-04-20 | 2021-07-09 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019515666A JP2019515666A (ja) | 2019-06-13 |
| JP2019515666A5 true JP2019515666A5 (enExample) | 2020-05-28 |
| JP7096770B2 JP7096770B2 (ja) | 2022-07-06 |
Family
ID=58701851
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018552794A Active JP7096770B2 (ja) | 2016-04-20 | 2017-04-20 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2021114374A Pending JP2021164479A (ja) | 2016-04-20 | 2021-07-09 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2023201924A Active JP7781128B2 (ja) | 2016-04-20 | 2023-11-29 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2025170326A Pending JP2025188169A (ja) | 2016-04-20 | 2025-10-08 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021114374A Pending JP2021164479A (ja) | 2016-04-20 | 2021-07-09 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2023201924A Active JP7781128B2 (ja) | 2016-04-20 | 2023-11-29 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
| JP2025170326A Pending JP2025188169A (ja) | 2016-04-20 | 2025-10-08 | 発現強化座位の使用に基づいた抗体を作製するための組成物および方法 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US11512144B2 (enExample) |
| EP (1) | EP3445781A1 (enExample) |
| JP (4) | JP7096770B2 (enExample) |
| KR (3) | KR102547738B1 (enExample) |
| CN (2) | CN116515757A (enExample) |
| AR (1) | AR109451A1 (enExample) |
| AU (2) | AU2017253241B2 (enExample) |
| BR (1) | BR112018071283A2 (enExample) |
| CA (1) | CA3015389A1 (enExample) |
| EA (1) | EA201892010A1 (enExample) |
| IL (2) | IL314779A (enExample) |
| MX (2) | MX2018012868A (enExample) |
| SG (2) | SG11201807885PA (enExample) |
| TW (1) | TWI827531B (enExample) |
| WO (1) | WO2017184832A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112018071283A2 (pt) * | 2016-04-20 | 2019-02-12 | Regeneron Pharma | célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno. |
| IL275462B2 (en) * | 2017-12-22 | 2026-01-01 | Genentech Inc | Targeted integration of nucleic acids |
| EP3980544A1 (en) * | 2018-12-21 | 2022-04-13 | F. Hoffmann-La Roche AG | Targeted integration of nucleic acids |
| SG11202110076PA (en) * | 2019-04-02 | 2021-10-28 | Chugai Pharmaceutical Co Ltd | Method of introducing target-specific foreign gene |
| EP3990649A1 (en) | 2019-06-26 | 2022-05-04 | Genentech, Inc. | Randomized configuration targeted integration of nucleic acids |
| AR127674A1 (es) | 2021-10-18 | 2024-02-21 | Regeneron Pharma | Células de mamífero que comprenden genes cas9 integrados para producir sitios de integración estables, y células de mamífero que comprenden sitios de integración estables y otros sitios |
| JP2024537896A (ja) | 2021-10-18 | 2024-10-16 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | アデノウイルス随伴ウイルスポリヌクレオチドを含む真核細胞 |
| CN118159660A (zh) | 2021-10-18 | 2024-06-07 | 瑞泽恩制药公司 | 多核苷酸的受控转录 |
| CN119731330A (zh) * | 2021-12-22 | 2025-03-28 | 基因泰克公司 | 多载体重组酶介导的盒式交换 |
| CN118974078A (zh) * | 2022-02-04 | 2024-11-15 | 小利兰·斯坦福大学董事会 | 替代细胞因子多肽 |
| CN119654417A (zh) * | 2022-06-10 | 2025-03-18 | 富士胶片戴奥辛思生物技术英国有限公司 | 细胞的制备方法、异源多聚体蛋白质的制造方法、双特异性抗体的制造方法、载体组、哺乳动物细胞、cho细胞和细胞池的制备方法 |
| CN121794393A (zh) | 2023-09-07 | 2026-04-03 | 里珍纳龙药品有限公司 | 经共价表面修饰的腺相关病毒的生产和纯化 |
| WO2025111473A1 (en) | 2023-11-21 | 2025-05-30 | Regeneron Pharmaceuticals, Inc. | Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus |
| WO2025115876A1 (ja) | 2023-11-29 | 2025-06-05 | 富士フイルム株式会社 | 細胞の作出方法、細胞及びタンパク質の製造方法 |
| KR20260103112A (ko) | 2023-11-29 | 2026-07-02 | 후지필름 가부시키가이샤 | 탐색 방법, 세포의 작출 방법, 세포의 선별 방법, 세포 및 세포 생산물의 제조 방법 |
| WO2026050618A1 (en) | 2024-08-29 | 2026-03-05 | Regeneron Pharmaceuticals, Inc. | Polynucleotide constructs and cell lines for recombinant aav production |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| US20020062010A1 (en) | 1997-05-02 | 2002-05-23 | Genentech, Inc. | Method for making multispecific antibodies having heteromultimeric and common components |
| US6800457B2 (en) | 1998-09-22 | 2004-10-05 | Bristol-Myers Squibb Company | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| US6406863B1 (en) | 2000-06-23 | 2002-06-18 | Genetastix Corporation | High throughput generation and screening of fully human antibody repertoire in yeast |
| CA2485939C (en) | 2002-05-29 | 2013-10-29 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
| US20050019925A1 (en) | 2002-11-14 | 2005-01-27 | Genentech, Inc. | Intron fusion construct and method of using for selecting high-expressing production cell lines |
| KR101374454B1 (ko) | 2005-03-31 | 2014-03-17 | 추가이 세이야쿠 가부시키가이샤 | 회합제어에 의한 폴리펩티드 제조방법 |
| DK1999154T3 (da) | 2006-03-24 | 2012-12-03 | Merck Patent Gmbh | Fremstillede heterodimere proteindomæner |
| ATE537190T1 (de) | 2006-06-02 | 2011-12-15 | Regeneron Pharma | Hochaffine antikörper gegen den humanen il-6- rezeptor |
| WO2008079943A2 (en) | 2006-12-21 | 2008-07-03 | Smithkline Beecham Corporation | Novel methods |
| US20080216185A1 (en) | 2007-01-19 | 2008-09-04 | Invitrogen Corporation | Compositions and Methods for Genetic Manipulation and Monitoring of Cell Lines |
| EP2626372B1 (en) | 2007-03-29 | 2018-03-21 | Genmab A/S | Bispecific antibodies and methods for production thereof |
| DK2150617T3 (en) * | 2007-06-04 | 2015-01-12 | Regeneron Pharma | Regions with increased expression and stability |
| EP3663318A1 (en) | 2008-01-07 | 2020-06-10 | Amgen Inc. | Method for making antibody fc-heterodimeric molecules using electrostatic steering effects |
| DK2808393T3 (en) * | 2009-06-02 | 2018-03-12 | Regeneron Pharma | Cells insufficient for fucosylation |
| TWI507525B (zh) | 2009-06-26 | 2015-11-11 | Regeneron Pharma | 具天然免疫球蛋白形式之易分離的雙專一性抗體 |
| CN102712696A (zh) | 2009-09-16 | 2012-10-03 | 弗·哈夫曼-拉罗切有限公司 | 包含卷曲螺旋和/或系链的蛋白质复合体及其用途 |
| CN102575264A (zh) * | 2009-09-18 | 2012-07-11 | 瑟莱克斯公司 | 增加的转基因表达和加工的产品和方法 |
| PT2560993T (pt) | 2010-04-20 | 2024-09-16 | Genmab As | Proteínas contendo anticorpo heterodimérico fc e métodos para a produção das mesmas |
| WO2012023053A2 (en) | 2010-08-16 | 2012-02-23 | Novimmune S.A. | Methods for the generation of multispecific and multivalent antibodies |
| EP2794662A1 (en) | 2011-12-22 | 2014-10-29 | F.Hoffmann-La Roche Ag | Full length antibody display system for eukaryotic cells and its use |
| TW201823460A (zh) | 2012-05-29 | 2018-07-01 | 美商再生元醫藥公司 | 生產細胞株增強子 |
| EP2711428A1 (en) | 2012-09-21 | 2014-03-26 | Lonza Biologics plc. | Site-specific integration |
| US20150203591A1 (en) | 2012-08-02 | 2015-07-23 | Regeneron Pharmaceuticals, Inc. | Mutivalent antigen-binding proteins |
| JOP20200236A1 (ar) | 2012-09-21 | 2017-06-16 | Regeneron Pharma | الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها |
| TWI864338B (zh) | 2012-11-14 | 2024-12-01 | 美商再生元醫藥公司 | 重組細胞表面捕捉蛋白質 |
| TWI682941B (zh) * | 2013-02-01 | 2020-01-21 | 美商再生元醫藥公司 | 含嵌合恆定區之抗體 |
| US20140308285A1 (en) | 2013-03-15 | 2014-10-16 | Amgen Inc. | Heterodimeric bispecific antibodies |
| TWI728373B (zh) | 2013-12-23 | 2021-05-21 | 美商建南德克公司 | 抗體及使用方法 |
| TWI754319B (zh) | 2014-03-19 | 2022-02-01 | 美商再生元醫藥公司 | 用於腫瘤治療之方法及抗體組成物 |
| EP3209785B1 (en) | 2014-10-23 | 2019-07-31 | Regeneron Pharmaceuticals, Inc. | Novel cho integration sites and uses thereof |
| MY195059A (en) | 2015-09-23 | 2023-01-05 | Regeneron Pharma | Optimized Anti-CD3 Bispecific Antibodies and Uses Thereof |
| BR112018071283A2 (pt) | 2016-04-20 | 2019-02-12 | Regeneron Pharma | célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno. |
-
2017
- 2017-04-20 BR BR112018071283A patent/BR112018071283A2/pt active Search and Examination
- 2017-04-20 JP JP2018552794A patent/JP7096770B2/ja active Active
- 2017-04-20 TW TW106113298A patent/TWI827531B/zh active
- 2017-04-20 EA EA201892010A patent/EA201892010A1/ru unknown
- 2017-04-20 KR KR1020187029365A patent/KR102547738B1/ko active Active
- 2017-04-20 US US16/095,084 patent/US11512144B2/en active Active
- 2017-04-20 WO PCT/US2017/028555 patent/WO2017184832A1/en not_active Ceased
- 2017-04-20 CN CN202211606177.7A patent/CN116515757A/zh active Pending
- 2017-04-20 EP EP17723183.4A patent/EP3445781A1/en active Pending
- 2017-04-20 KR KR1020237020953A patent/KR102906454B1/ko active Active
- 2017-04-20 IL IL314779A patent/IL314779A/en unknown
- 2017-04-20 AR ARP170101019A patent/AR109451A1/es unknown
- 2017-04-20 MX MX2018012868A patent/MX2018012868A/es unknown
- 2017-04-20 CN CN201780024557.0A patent/CN109195986B/zh active Active
- 2017-04-20 KR KR1020257043281A patent/KR20260011194A/ko active Pending
- 2017-04-20 CA CA3015389A patent/CA3015389A1/en active Pending
- 2017-04-20 SG SG11201807885PA patent/SG11201807885PA/en unknown
- 2017-04-20 IL IL262266A patent/IL262266B2/en unknown
- 2017-04-20 SG SG10202010155YA patent/SG10202010155YA/en unknown
- 2017-04-20 AU AU2017253241A patent/AU2017253241B2/en active Active
-
2018
- 2018-10-19 MX MX2025003852A patent/MX2025003852A/es unknown
-
2021
- 2021-07-09 JP JP2021114374A patent/JP2021164479A/ja active Pending
-
2022
- 2022-09-28 US US17/936,059 patent/US12528883B2/en active Active
-
2023
- 2023-11-29 JP JP2023201924A patent/JP7781128B2/ja active Active
-
2024
- 2024-09-26 AU AU2024220124A patent/AU2024220124A1/en active Pending
-
2025
- 2025-10-08 JP JP2025170326A patent/JP2025188169A/ja active Pending
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