EA201892010A1 - Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию - Google Patents

Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию

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Publication number
EA201892010A1
EA201892010A1 EA201892010A EA201892010A EA201892010A1 EA 201892010 A1 EA201892010 A1 EA 201892010A1 EA 201892010 A EA201892010 A EA 201892010A EA 201892010 A EA201892010 A EA 201892010A EA 201892010 A1 EA201892010 A1 EA 201892010A1
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Eurasian Patent Office
Prior art keywords
methods
compositions
locuses
enhanced expression
application
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EA201892010A
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English (en)
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Роберт Бабб
Дарья Бураков
Ганг Чен
Джеймс П. Фандл
Ю Чжао
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Регенерон Фармасьютикалз, Инк.
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Publication of EA201892010A1 publication Critical patent/EA201892010A1/ru

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    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/526—CH3 domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates
    • C12N2800/107—Plasmid DNA for vertebrates for mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Настоящее изобретение относится к сайт-специфической интеграции и экспрессии рекомбинантных белков в эукариотических клетках. В частности, настоящее изобретение включает композиции и способы для обеспечения улучшенной экспрессии антигенсвязывающих белков, включая моноспецифические и биспецифические антитела, в эукариотических клетках, в частности, в линиях клеток китайского хомячка (Cricetulus griseus), посредством применения нескольких локусов, обеспечивающих повышенную экспрессию.
EA201892010A 2016-04-20 2017-04-20 Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию EA201892010A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662325400P 2016-04-20 2016-04-20
PCT/US2017/028555 WO2017184832A1 (en) 2016-04-20 2017-04-20 Compositions and methods for making antibodies based on use of expression-enhancing loci

Publications (1)

Publication Number Publication Date
EA201892010A1 true EA201892010A1 (ru) 2019-03-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201892010A EA201892010A1 (ru) 2016-04-20 2017-04-20 Композиции и способы получения антител, основанные на применении локусов, обеспечивающих повышенную экспрессию

Country Status (15)

Country Link
US (2) US11512144B2 (ru)
EP (1) EP3445781A1 (ru)
JP (3) JP7096770B2 (ru)
KR (2) KR20230098361A (ru)
CN (2) CN109195986B (ru)
AR (1) AR109451A1 (ru)
AU (1) AU2017253241B2 (ru)
BR (1) BR112018071283A2 (ru)
CA (1) CA3015389A1 (ru)
EA (1) EA201892010A1 (ru)
IL (1) IL262266A (ru)
MX (1) MX2018012868A (ru)
SG (2) SG11201807885PA (ru)
TW (2) TW202415767A (ru)
WO (1) WO2017184832A1 (ru)

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Also Published As

Publication number Publication date
US11512144B2 (en) 2022-11-29
CN109195986A (zh) 2019-01-11
KR20180134893A (ko) 2018-12-19
JP2019515666A (ja) 2019-06-13
EP3445781A1 (en) 2019-02-27
CN116515757A (zh) 2023-08-01
AU2017253241A1 (en) 2018-09-20
KR20230098361A (ko) 2023-07-03
JP2021164479A (ja) 2021-10-14
US20190233544A1 (en) 2019-08-01
WO2017184832A1 (en) 2017-10-26
AR109451A1 (es) 2018-12-12
AU2017253241B2 (en) 2024-07-04
CA3015389A1 (en) 2017-10-26
US20230130799A1 (en) 2023-04-27
JP7096770B2 (ja) 2022-07-06
KR102547738B1 (ko) 2023-06-26
TWI827531B (zh) 2024-01-01
IL262266A (en) 2018-11-29
TW201803986A (zh) 2018-02-01
CN109195986B (zh) 2023-01-03
JP2024023449A (ja) 2024-02-21
MX2018012868A (es) 2019-03-11
TW202415767A (zh) 2024-04-16
BR112018071283A2 (pt) 2019-02-12
SG11201807885PA (en) 2018-10-30
SG10202010155YA (en) 2020-11-27

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