JP2018508198A - 別個の実体におけるバーコード付加による核酸のシーケンシング - Google Patents

別個の実体におけるバーコード付加による核酸のシーケンシング Download PDF

Info

Publication number
JP2018508198A
JP2018508198A JP2017539228A JP2017539228A JP2018508198A JP 2018508198 A JP2018508198 A JP 2018508198A JP 2017539228 A JP2017539228 A JP 2017539228A JP 2017539228 A JP2017539228 A JP 2017539228A JP 2018508198 A JP2018508198 A JP 2018508198A
Authority
JP
Japan
Prior art keywords
nucleic acid
sequence
separate
acid barcode
entity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2017539228A
Other languages
English (en)
Japanese (ja)
Other versions
JP2018508198A5 (OSRAM
Inventor
アール. アベート,アダム
アール. アベート,アダム
ハリバートン,ジョン
ラン,フリーマン
アール. シャンビ,アダム
アール. シャンビ,アダム
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
University of California Berkeley
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of JP2018508198A publication Critical patent/JP2018508198A/ja
Publication of JP2018508198A5 publication Critical patent/JP2018508198A5/ja
Priority to JP2022023107A priority Critical patent/JP2022088360A/ja
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40Static mixers
    • B01F25/42Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4338Mixers with a succession of converging-diverging cross-sections, i.e. undulating cross-section
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30Micromixers
    • B01F33/302Micromixers the materials to be mixed flowing in the form of droplets
    • B01F33/3021Micromixers the materials to be mixed flowing in the form of droplets the components to be mixed being combined in a single independent droplet, e.g. these droplets being divided by a non-miscible fluid or consisting of independent droplets
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N15/0205Investigating particle size or size distribution by optical means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404Handling flow, e.g. hydrodynamic focusing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01FMIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F21/00Dissolving
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/02Identification, exchange or storage of information
    • B01L2300/021Identification, e.g. bar codes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0627Sensor or part of a sensor is integrated
    • B01L2300/0645Electrodes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867Multiple inlets and one sample wells, e.g. mixing, dilution
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/12Specific details about materials
    • B01L2300/123Flexible; Elastomeric
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/149Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N2015/0288Sorting the particles
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N2015/0294Particle shape
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493Particle size
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1497Particle shape

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
JP2017539228A 2015-02-04 2016-02-03 別個の実体におけるバーコード付加による核酸のシーケンシング Pending JP2018508198A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2022023107A JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562112075P 2015-02-04 2015-02-04
US62/112,075 2015-02-04
PCT/US2016/016444 WO2016126871A2 (en) 2015-02-04 2016-02-03 Sequencing of nucleic acids via barcoding in discrete entities

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2022023107A Division JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Publications (2)

Publication Number Publication Date
JP2018508198A true JP2018508198A (ja) 2018-03-29
JP2018508198A5 JP2018508198A5 (OSRAM) 2019-03-14

Family

ID=56564869

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2017539228A Pending JP2018508198A (ja) 2015-02-04 2016-02-03 別個の実体におけるバーコード付加による核酸のシーケンシング
JP2022023107A Pending JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2022023107A Pending JP2022088360A (ja) 2015-02-04 2022-02-17 別個の実体におけるバーコード付加による核酸のシーケンシング

Country Status (7)

Country Link
US (3) US11111519B2 (OSRAM)
EP (2) EP3253479B1 (OSRAM)
JP (2) JP2018508198A (OSRAM)
CN (2) CN115011670A (OSRAM)
AU (2) AU2016215304B2 (OSRAM)
CA (1) CA2974306A1 (OSRAM)
WO (1) WO2016126871A2 (OSRAM)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018014924A (ja) * 2016-07-27 2018-02-01 国立大学法人京都大学 ヘリカーゼを用いたpcr
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
WO2021241721A1 (ja) * 2020-05-29 2021-12-02 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JP2021534956A (ja) * 2018-08-17 2021-12-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 安定なジェットからの単分散粒子に誘発される液滴の形成
JP2022522221A (ja) * 2019-04-02 2022-04-14 ミッション バイオ インコーポレイテッド 腫瘍を特性決定し、腫瘍の不均質性を識別するための方法及びシステム
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis

Families Citing this family (218)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
US10564147B2 (en) 2012-05-25 2020-02-18 The Regents Of The University Of California Microfluidic systems for particle trapping and separation using cavity acoustic transducers
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) * 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014124338A1 (en) 2013-02-08 2014-08-14 10X Technologies, Inc. Polynucleotide barcode generation
US11859171B2 (en) 2013-04-17 2024-01-02 Agency For Science, Technology And Research Method for generating extended sequence reads
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN114534806B (zh) 2014-04-10 2024-03-29 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN106536756A (zh) 2014-06-26 2017-03-22 10X基因组学有限公司 核酸序列的分析
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
GB201414177D0 (en) * 2014-08-11 2014-09-24 Sphere Fluidics Ltd Droplet Sorting
CN114807307A (zh) 2014-10-29 2022-07-29 10X 基因组学有限公司 用于靶核酸测序的方法和组合物
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
HK1250245A1 (zh) 2015-04-10 2018-12-07 Spatial Transcriptomics Ab 生物样本的空间区别、多重核酸分析
HK1252248A1 (zh) * 2015-04-27 2019-05-24 Abvitro Llc 对治疗剂和疾病特异性抗原进行测序、确定、配对和验证的方法
GB201509640D0 (en) * 2015-06-03 2015-07-15 Sphere Fluidics Ltd Systems and methods
EP3337907A4 (en) * 2015-08-17 2019-01-02 The Regents of The University of California Microdroplet-based multiple displacement amplification (mda) methods and related compositions
US9862941B2 (en) 2015-10-14 2018-01-09 Pioneer Hi-Bred International, Inc. Single cell microfluidic device
KR20200103895A (ko) * 2015-11-10 2020-09-02 일루미나, 인코포레이티드 관성 소적 생성 및 입자 캡슐화
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
SG11201804086VA (en) 2015-12-04 2018-06-28 10X Genomics Inc Methods and compositions for nucleic acid analysis
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
US11702702B2 (en) 2016-04-15 2023-07-18 Predicine, Inc. Systems and methods for detecting genetic alterations
WO2018183796A1 (en) * 2017-03-31 2018-10-04 Predicine, Inc. Systems and methods for predicting and monitoring cancer therapy
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109563539A (zh) 2016-06-15 2019-04-02 慕尼黑路德维希马克西米利安斯大学 使用dna纳米技术的单分子检测或定量
EP3485215B1 (en) 2016-07-12 2023-06-07 Alexander Poltorak System and method for maintaining efficiency of a heat sink
EP3512945A4 (en) 2016-09-15 2020-08-26 Augmenta Bioworks, Inc. METHODS AND APPLICATIONS OF AMPLIFICATION OF IMMUNOLOGICAL DIRECTORY SEQUENCES
US11174503B2 (en) 2016-09-21 2021-11-16 Predicine, Inc. Systems and methods for combined detection of genetic alterations
KR102522023B1 (ko) 2016-09-26 2023-04-17 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
WO2018067792A1 (en) * 2016-10-07 2018-04-12 President And Fellows Of Harvard College Sequencing of bacteria or other species
EP3525933B1 (en) * 2016-10-11 2024-07-03 The Regents of the University of California Systems and methods to encapsulate and preserve organic matter for analysis
EP3538662B1 (en) * 2016-11-10 2021-12-01 Takara Bio USA, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein
AU2017363146B2 (en) * 2016-11-16 2023-11-02 Catalog Technologies, Inc. Systems for nucleic acid-based data storage
CN110114473A (zh) * 2016-11-23 2019-08-09 斯特拉斯堡大学 靶分子的串联条形码添加以便以单实体分辨率对靶分子进行绝对定量
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3545089B1 (en) 2017-01-30 2022-03-09 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
EP3838268B1 (en) * 2017-02-24 2023-05-10 The Regents of the University of California Particle-drop structures and methods for making and using the same
CN107083423B (zh) * 2017-03-27 2022-01-28 北京极客基因科技有限公司 一种药物靶点预测和药物全方面评价方法
WO2018191701A1 (en) * 2017-04-14 2018-10-18 The Broad Institute, Inc. High-throughput screens for exploring biological functions of microscale biological systems
WO2018213643A1 (en) 2017-05-18 2018-11-22 10X Genomics, Inc. Methods and systems for sorting droplets and beads
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP3631012B1 (en) * 2017-05-26 2022-06-08 AbVitro LLC High-throughput polynucleotide library sequencing and transcriptome analysis
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
KR102790050B1 (ko) 2017-06-05 2025-04-04 백톤 디킨슨 앤드 컴퍼니 단일 세포를 위한 샘플 인덱싱
WO2018227210A1 (en) 2017-06-09 2018-12-13 The Regents Of The University Of California High-efficiency encapsulation in droplets based on hydrodynamic vortices control
US11517901B2 (en) 2017-06-09 2022-12-06 The Regents Of The University Of California High-efficiency particle encapsulation in droplets with particle spacing and downstream droplet sorting
WO2018236918A1 (en) 2017-06-20 2018-12-27 Bio-Rad Laboratories, Inc. MDA USING A BALL OLIGONUCLEOTIDE
TWI637834B (zh) * 2017-07-11 2018-10-11 台灣奈米碳素股份有限公司 微流道裝置的製造方法及其結構
EP3655718A4 (en) 2017-07-17 2021-03-17 Alexander Poltorak MULTIFRACTAL HEAT SINK SYSTEM AND PROCESS
EP3665280A4 (en) * 2017-08-10 2021-10-06 Metabiotech Corporation MARKING OF NUCLEIC ACID MOLECULES OF INDIVIDUAL CELLS FOR STEP SEQUENCING
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
ES2994431T3 (en) 2017-09-22 2025-01-23 Univ Washington In situ combinatorial labeling of cellular molecules
EP4462123A3 (en) 2017-09-27 2025-02-12 Bio-Rad Laboratories, Inc. Digital affinity linkage assay
EP4494757A3 (en) 2017-09-29 2025-03-19 The Regents of The University of California Method of generating monodisperse emulsions
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019075409A1 (en) 2017-10-12 2019-04-18 The Regents Of The University Of California ISOLATION AND IDENTIFICATION WITHOUT MICROFLUIDIC LABEL OF CELLS USING FLUORESCENCE LIFE IMAGING (FLIM)
US20190112655A1 (en) * 2017-10-18 2019-04-18 Mission Bio, Inc. Method, Systems and Apparatus for High-Throughput Single-Cell DNA Sequencing With Droplet Microfluidics
US11499127B2 (en) 2017-10-20 2022-11-15 The Regents Of The University Of California Multi-layered microfluidic systems for in vitro large-scale perfused capillary networks
WO2019079787A1 (en) 2017-10-20 2019-04-25 The Regents Of The University Of California MICROFLUIDIC SYSTEMS AND METHODS FOR CELL TRANSFECTION VIA LIPOFLEX
WO2019084055A1 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. MICROFLUIDIC CHANNEL NETWORKS FOR PARTITIONING
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
CN114525273B (zh) 2017-10-27 2025-01-28 10X基因组学有限公司 用于样品制备和分析的方法和系统
CN111051523B (zh) 2017-11-15 2024-03-19 10X基因组学有限公司 功能化凝胶珠
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019104337A1 (en) 2017-11-27 2019-05-31 The Trustees Of Columbia University In The City Of New York Rna printing and sequencing devices, methods, and systems
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019113235A1 (en) * 2017-12-06 2019-06-13 10X Genomics, Inc. Methods and systems for processing nucleic acid molecules
EP3724658A1 (en) 2017-12-12 2020-10-21 10X Genomics, Inc. Systems and methods for single cell processing
CN118547046A (zh) 2017-12-22 2024-08-27 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
CN111556900A (zh) * 2017-12-22 2020-08-18 赛默飞世尔科技波罗的海封闭股份公司 包括胺的聚合酶链反应组合物
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) * 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
JP7573444B2 (ja) * 2018-03-02 2024-10-25 クァンタムサイト, インコーポレイテッド 組織からの目的の領域の隔離および発現分析のための方法、組成物、およびデバイス
EP3762494B1 (en) * 2018-03-07 2022-11-23 Wisconsin Alumni Research Foundation High throughput nucleic acid profiling of single cells
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
SG11201911961RA (en) 2018-04-20 2020-01-30 Illumina Inc Methods of encapsulating single cells, the encapsulated cells and uses thereof
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
WO2019224219A1 (en) * 2018-05-22 2019-11-28 Universite De Strasbourg Absolute quantification of target molecules at single-entity resolution using tandem barcoding
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
WO2020009998A1 (en) * 2018-07-03 2020-01-09 Chan Zuckerberg Biohub, Inc. Method to perform high-throughput single cell genomic and phenotypic analyses
CN112654713A (zh) * 2018-07-05 2021-04-13 安可济控股有限公司 用于数字聚合酶链反应的组合物和方法
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028912A2 (en) 2018-08-03 2020-02-06 Catolog Technologies, Inc Systems and methods for storing and reading nucleic acid-based data with error protection
CN112703252B (zh) 2018-08-03 2024-09-10 10X基因组学有限公司 用于最小化条形码交换的方法和系统
US20200048626A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for Making Gel Beads and Core and Shell Beads with a Cell
US20210301247A1 (en) * 2018-08-10 2021-09-30 Cellino Biotech, Inc. System for image-driven cell manufacturing
US12065688B2 (en) * 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
EP4471156A3 (en) 2018-10-01 2025-02-26 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020077017A2 (en) 2018-10-10 2020-04-16 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
US11608524B1 (en) * 2018-10-25 2023-03-21 Wisconsin Alumni Research Foundation Methods of analyzing cells
EP4656734A2 (en) * 2018-10-26 2025-12-03 Illumina, Inc. Modulating polymer beads for dna processing
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
WO2020123318A1 (en) * 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
CN111378557B (zh) 2018-12-26 2023-06-06 财团法人工业技术研究院 用于产生液珠的管状结构及液珠产生方法
EP3902922A1 (en) * 2018-12-28 2021-11-03 Biobloxx AB Method and kit for preparing complementary dna
CN113874521B (zh) * 2019-01-06 2024-11-01 10X基因组学有限公司 用于富集条形码的方法和系统
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US20200232011A1 (en) * 2019-01-22 2020-07-23 Mission Bio Methods of nucleic acid detection and primer design
WO2020167866A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transposon loading
CN118979095A (zh) 2019-02-12 2024-11-19 10X基因组学有限公司 用于加工核酸分子的方法
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
WO2020167862A1 (en) 2019-02-12 2020-08-20 10X Genomics, Inc. Systems and methods for transfer of reagents between droplets
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
CN113767178A (zh) 2019-03-11 2021-12-07 10X基因组学有限公司 用于处理光学标签化珠粒的系统和方法
WO2020183591A1 (en) * 2019-03-11 2020-09-17 Enplas Corporation Fluid handling method, and fluid handling device and fluid handling system used for the method
CA3132030A1 (en) * 2019-04-02 2020-10-08 Dalia Dhingra Methods, systems, and apparatus for nucleic acid detection
WO2020252387A2 (en) * 2019-06-12 2020-12-17 Ultima Genomics, Inc. Methods for accurate base calling using molecular barcodes
CN114391042A (zh) * 2019-06-27 2022-04-22 细胞微系统有限公司 用于将单细胞成像与rna转录组学相关联的系统和方法
US12186751B2 (en) 2019-06-28 2025-01-07 10X Genomics, Inc. Devices and systems incorporating acoustic ordering and methods of use thereof
CN113891961A (zh) * 2019-07-12 2022-01-04 深圳华大生命科学研究院 一种全基因组全流程微流控自动化建库方法和装置
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
KR20220041875A (ko) * 2019-07-31 2022-04-01 바이오스크립 지노믹스, 인크. 단일 세포 분석
WO2021026228A1 (en) 2019-08-05 2021-02-11 Mission Bio, Inc. Method and apparatus for single-cell analysis for determining a cell trajectory
US12179199B2 (en) 2019-08-09 2024-12-31 The Regents Of The University Of California Microfluidic single-cell pairing array for studying cell-cell interactions in isolated compartments
WO2021046299A1 (en) * 2019-09-04 2021-03-11 Vanderbilt University Methods for identification of antigen binding specificity of antibodies
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
WO2021067286A1 (en) * 2019-09-30 2021-04-08 Shield Diagnostics Corp. Devices for determining a target nucleic acid profile and methods of use thereof
WO2021067966A1 (en) * 2019-10-05 2021-04-08 Mission Bio, Inc. Methods, systems and apparatus for copy number variations and single nucleotide variations simultaneously detected in single-cells
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
US20220381781A1 (en) * 2019-10-16 2022-12-01 Georgia Tech Research Corporation Methods for ultrasensitive detection of protein and cellular biomarkers
CN110777048A (zh) * 2019-11-01 2020-02-11 上海市第六人民医院 一种高通量单外泌体分离装置
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
US20220364147A1 (en) * 2019-11-15 2022-11-17 Miltenyi Biotec B.V. & Co. KG Color and bardcoded beads for single cell indexing
US12059679B2 (en) * 2019-11-19 2024-08-13 10X Genomics, Inc. Methods and devices for sorting droplets and particles
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
WO2021128035A1 (zh) * 2019-12-25 2021-07-01 苏州绘真生物科技有限公司 高通量单细胞转录组测序方法及试剂盒
CN115768558A (zh) 2020-01-13 2023-03-07 福路伦特生物科学公司 用于单细胞基因谱分析的方法和系统
JP2023511279A (ja) 2020-01-13 2023-03-17 フルーエント バイオサイエンシーズ インコーポレイテッド 単一細胞シーケンシング
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
US11512337B2 (en) 2020-01-13 2022-11-29 Fluent Biosciences Inc. Emulsion based drug screening
CN114945684A (zh) * 2020-01-14 2022-08-26 哈佛学院院长及董事 使用数字微滴式pcr和相关技术测定核酸的装置和方法
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
EP4095256A4 (en) * 2020-01-23 2023-03-15 DGI Tech (Qing Dao) Co., Limited Droplet microfluidics-based single cell sequencing and applications
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12416037B2 (en) 2020-01-24 2025-09-16 Vanderbilt University Methods for identification of ligand-blocking antibodies and for determining antibody potency
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12449419B1 (en) 2020-02-12 2025-10-21 10X Genomics, Inc. Methods for detecting binding of peptide-MHC monomers to T cells
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11866782B2 (en) 2020-03-16 2024-01-09 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
CN111454942A (zh) * 2020-03-16 2020-07-28 张晓鲁 一种同一样品的转录组和基因组同时建立测序文库的构建方法
WO2021188889A1 (en) 2020-03-20 2021-09-23 Mission Bio, Inc. Single cell workflow for whole genome amplification
US12428685B2 (en) 2020-03-24 2025-09-30 Illumina, Inc. Viral detection using template emulsification
CN111394432B (zh) * 2020-03-27 2023-03-24 深圳闪量科技有限公司 基于通用探针芯片的多重定量pcr检测系统
EP4143333A1 (en) 2020-04-30 2023-03-08 Dimensiongen Devices and methods for macromolecular manipulation
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
GB202007059D0 (en) * 2020-05-13 2020-06-24 Univ Oxford Innovation Ltd Polynucleotide arrays
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4158055B1 (en) 2020-06-02 2024-03-27 Becton, Dickinson and Company Oligonucleotides and beads for 5 prime gene expression assay
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CA3189597A1 (en) 2020-07-15 2022-01-20 Fluent Biosciences Inc. Tiered ligation oligos
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN114073995A (zh) * 2020-08-13 2022-02-22 安行生物技术有限公司 分析单细胞的微流控设备和单元及其方法
GB202012730D0 (en) * 2020-08-14 2020-09-30 Cambridge Entpr Ltd Modular microfluidic devices, systems and methodds for total RNA analyses
US12480158B1 (en) 2020-11-05 2025-11-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022099208A1 (en) * 2020-11-09 2022-05-12 The General Hospital Corporation High throughput microfluidics for analysis of immune cell activation
EP4244379A1 (en) * 2020-11-13 2023-09-20 10X Genomics, Inc. Nano-partitions (encapsulated nucleic acid processing enzymes) for cell-lysis and multiple reactions in partition-based assays
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US20220243264A1 (en) * 2020-11-23 2022-08-04 The Broad Institute, Inc. Systems and methods for amplifying rna
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
CN113322254B (zh) * 2021-01-06 2022-05-20 南京诺唯赞生物科技股份有限公司 多靶点蛋白质-dna相互作用的研究方法和工具
US12398262B1 (en) 2021-01-22 2025-08-26 10X Genomics, Inc. Triblock copolymer-based cell stabilization and fixation system and methods of use thereof
CN114836838A (zh) * 2021-02-01 2022-08-02 南方医科大学 一种中通量单细胞拷贝数文库构建的方法及其应用
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
US20240209350A1 (en) * 2021-04-26 2024-06-27 European Molecular Biology Laboratory High complexity microcompartment-based interaction screening
WO2023044307A1 (en) * 2021-09-14 2023-03-23 Becton, Dickinson And Company Full length single cell rna sequencing
WO2023154816A1 (en) * 2022-02-09 2023-08-17 Mission Bio, Inc. Systems and methods of detecting merged droplets in single cell sequencing
DK181862B1 (en) * 2022-03-21 2025-02-24 Samplix Aps Method and kit for targeted enrichment of large dna molecules for long-read sequencing using facs or microfluidic partitioning
US20250297243A1 (en) * 2022-04-28 2025-09-25 BioSkryb Genomics, Inc. Single cell multiomics
EP4540607A1 (en) 2022-06-17 2025-04-23 10X Genomics, Inc. Catalytic de-crosslinking of samples for in situ analysis
EP4612283A1 (en) * 2022-11-04 2025-09-10 Fluid Discovery Systems, compositions, and methods for single cell analysis
EP4658409A1 (en) * 2023-01-30 2025-12-10 IDEXX Laboratories, Inc. Methods, systems, and device for electrowetting bar-coded magnetic beads
WO2025040497A1 (en) * 2023-08-18 2025-02-27 Qiagen Gmbh Method for improving nucleic acid processing
CN120783873B (zh) * 2025-07-09 2025-12-05 上海锦测医学检验所有限公司 一种智能化蛋白质组学数据异常检测与分析方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140272988A1 (en) * 2013-03-14 2014-09-18 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0680335A1 (en) 1992-11-30 1995-11-08 The Wellcome Foundation Limited Sequential targeting of tumor sites with oligonucleotide conjugates of antibody and complementary oligonucleotide conjugates of chelated radionuclide
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US6319507B1 (en) 1997-05-02 2001-11-20 Kobo Products, Inc. Agar gel bead composition and method
DE69826697T2 (de) 1997-07-07 2006-02-16 Medical Research Council In vitro selektionsmethode
US6436677B1 (en) 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
US7041208B2 (en) 2001-12-11 2006-05-09 Sau Lan Tang Staats Microfluidic devices and methods for two-dimensional separations
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
DE10339452A1 (de) 2003-08-22 2005-03-17 Institut für Physikalische Hochtechnologie e.V. Vorrichtung und Verfahren zur Strukturierung von Flüssigkeiten
EP2662135A3 (en) 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for mixing droplets in a microchannel
CN101415838A (zh) 2003-09-26 2009-04-22 首科安普有限公司 用滚环扩增法扩增多核苷酸
EP1691792A4 (en) 2003-11-24 2008-05-28 Yeda Res & Dev COMPOSITIONS AND PROCESSES FOR SORTING IN VITRO OF MOLECULE AND CELL LIBRARIES
KR100647282B1 (ko) 2003-12-24 2006-11-17 삼성전자주식회사 DNA 마이크로어레이 스폿팅(Spoting) 장치 및이를 이용한 스폿팅 방법
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US20060201868A1 (en) 2005-03-11 2006-09-14 Simmons Blake A Methods and devices for high-throughput dielectrophoretic concentration
EP2660482B1 (en) 2005-08-22 2019-08-07 Life Technologies Corporation Vorrichtung, System und Verfahren unter Verwendung von nichtmischbaren Flüssigkeiten mit unterschiedlichen Volumen
US8383059B2 (en) 2005-09-30 2013-02-26 University Of Utah Research Foundation Microfluidic interface for highly parallel addressing of sensing arrays
US9183349B2 (en) 2005-12-16 2015-11-10 Nextbio Sequence-centric scientific information management
JP2009536313A (ja) 2006-01-11 2009-10-08 レインダンス テクノロジーズ, インコーポレイテッド ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法
JP5180845B2 (ja) * 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッド Dnaアレイ上でのハイスループットゲノム配列決定
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
WO2007140015A2 (en) * 2006-05-26 2007-12-06 Althea Technologies, Inc Biochemical analysis of partitioned cells
DK2027470T3 (da) 2006-06-02 2013-02-04 Pfizer Prod Inc Analyse for cirkulerende tumorceller
NZ597995A (en) 2006-12-14 2013-05-31 Dow Agrosciences Llc Optimized non-canonical zinc finger proteins
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
WO2009015296A1 (en) 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
GB0720202D0 (en) * 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
US9138700B2 (en) 2008-01-18 2015-09-22 The Regents Of The University Of California Accurate and rapid micromixer for integrated microfluidic devices
CA2717070C (en) 2008-03-04 2019-12-31 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
US9409177B2 (en) 2008-03-21 2016-08-09 Lawrence Livermore National Security, Llc Chip-based device for parallel sorting, amplification, detection, and identification of nucleic acid subsequences
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20140179544A1 (en) 2012-09-07 2014-06-26 Bio-Rad Laboratories, Inc. Compositions, systems and methods for droplet formation, spacing and detection
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
WO2013016459A1 (en) 2011-07-25 2013-01-31 Bio-Rad Laboratories, Inc. Breakage of an emulsion containing nucleic acid
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
JP5457222B2 (ja) 2009-02-25 2014-04-02 エフ.ホフマン−ラ ロシュ アーゲー 小型化ハイスループット核酸分析
EP2977455B1 (en) 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
SG177369A1 (en) 2009-06-26 2012-02-28 Harvard College Fluid injection
CA3106547C (en) 2009-09-02 2022-07-05 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
US9625454B2 (en) * 2009-09-04 2017-04-18 The Research Foundation For The State University Of New York Rapid and continuous analyte processing in droplet microfluidic devices
US8746285B2 (en) 2009-09-04 2014-06-10 Auburn University Programmable fluidic droplet generation
US9376713B2 (en) 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
ES2628739T3 (es) 2009-10-15 2017-08-03 Ibis Biosciences, Inc. Amplificación por desplazamiento múltiple
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
AU2013203624B2 (en) 2010-02-09 2015-06-25 General Electric Company Isothermal amplification of nucleic acid using a mixture of randomized primers and specific primers
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
CA2767182C (en) 2010-03-25 2020-03-24 Bio-Rad Laboratories, Inc. Droplet generation for droplet-based assays
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
JP5573335B2 (ja) 2010-04-28 2014-08-20 株式会社島津製作所 磁性体粒子操作デバイス及び磁性体粒子操作方法
DK2577254T3 (en) 2010-06-10 2015-06-01 Univ Freiburg Albert Ludwigs An apparatus and method for delivering cells or particles that are encased in a freely suspended droplet
US9650629B2 (en) 2010-07-07 2017-05-16 Roche Molecular Systems, Inc. Clonal pre-amplification in emulsion
AU2011281183B2 (en) 2010-07-22 2015-05-14 Gencell Biosystems Limited Composite liquid cells
US9695390B2 (en) 2010-08-23 2017-07-04 President And Fellows Of Harvard College Acoustic waves in microfluidics
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
EP2436444A1 (en) 2010-10-01 2012-04-04 Centre National de la Recherche Scientifique (C.N.R.S.) Microfluidic device for production and collection of droplets of a fluid
EP2625320B1 (en) 2010-10-08 2019-03-27 President and Fellows of Harvard College High-throughput single cell barcoding
US20130035259A1 (en) 2010-11-22 2013-02-07 The University Of Chicago Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections
DK2652155T3 (en) * 2010-12-16 2017-02-13 Gigagen Inc Methods for Massive Parallel Analysis of Nucleic Acids in Single Cells
WO2012102663A1 (en) 2011-01-24 2012-08-02 Ge Healthcare Bio-Sciences Ab Method of coupling binding agents to a substrate surface
US8765455B2 (en) 2011-01-27 2014-07-01 Lawrence Livermore National Security, Llc Chip-based droplet sorting
ES2687761T3 (es) * 2011-01-31 2018-10-29 F. Hoffmann-La Roche Ag Métodos de identificación de múltiples epítopos en células
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
CN110016499B (zh) 2011-04-15 2023-11-14 约翰·霍普金斯大学 安全测序系统
EP3395957B1 (en) 2011-04-25 2020-08-12 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
SG194745A1 (en) 2011-05-20 2013-12-30 Fluidigm Corp Nucleic acid encoding reactions
BR112013029729A2 (pt) 2011-05-23 2017-01-24 Basf Se controle de emulsões, incluindo emulsões múltiplas
EP2714970B1 (en) * 2011-06-02 2017-04-19 Raindance Technologies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CN103003447B (zh) 2011-07-26 2020-08-25 维里纳塔健康公司 用于确定样品中存在或不存在不同非整倍性的方法
US20130032235A1 (en) 2011-08-02 2013-02-07 Teledyne Dalsa Semiconductor, Inc. Integrated microfluidic check valve and device including such a check valve
US9469874B2 (en) * 2011-10-18 2016-10-18 The Regents Of The University Of California Long-range barcode labeling-sequencing
US9483374B2 (en) 2011-12-21 2016-11-01 Intel Corporation PSMI using at-speed scan capture
JP2015513451A (ja) 2012-02-08 2015-05-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 流体分割を用いる液滴形成
WO2013126741A1 (en) 2012-02-24 2013-08-29 Raindance Technologies, Inc. Labeling and sample preparation for sequencing
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
ES2899687T3 (es) 2012-05-14 2022-03-14 Irepertoire Inc Método para aumentar la precisión en la detección cuantitativa de polinucleótidos
AU2013302867A1 (en) 2012-08-13 2015-02-26 The Regents Of The University Of California Methods and systems for detecting biological components
CN111748607B (zh) 2012-08-14 2024-04-30 10X基因组学有限公司 微胶囊组合物及方法
US20140378349A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
EP2898071A4 (en) 2012-09-21 2016-07-20 Broad Inst Inc COMPOSITIONS AND METHODS FOR LONG-TERM LABORATORIES AND PREPARED END OF NUCLEIC ACIDS IN EMULSION DROPS
CA2888042A1 (en) 2012-11-27 2014-06-05 Gencell Biosystems Ltd. Handling liquid samples
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014108323A1 (en) 2013-01-10 2014-07-17 Eth Zurich Method and apparatus for depositing droplets onto a substrate
WO2014138132A2 (en) 2013-03-04 2014-09-12 Verax Biomedical Incorporated Multi-analyte assay
WO2014153071A1 (en) 2013-03-14 2014-09-25 The Broad Institute, Inc. Methods for quantitating dna using digital multiple displacement amplification
CN105452873B (zh) 2013-03-15 2019-01-18 加利福尼亚大学董事会 高速按需微流体珠滴生成和操控
GB2525568B (en) * 2013-03-15 2020-10-14 Abvitro Llc Single cell barcoding for antibody discovery
DE102013207900A1 (de) 2013-04-30 2014-10-30 Volkswagen Ag Membran-Elektroden-Einheit und Brennstoffzelle mit einer solchen
ES2857908T3 (es) 2013-08-28 2021-09-29 Becton Dickinson Co Análisis masivamente paralelo de células individuales
WO2015069798A1 (en) 2013-11-05 2015-05-14 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
WO2015120398A1 (en) 2014-02-10 2015-08-13 Gencell Biosystems Limited Composite liquid cell (clc) mediated nucleic acid library preparation device, and methods for using the same
US10619192B2 (en) 2014-04-08 2020-04-14 University Of Washington Through Its Center For Commercialization Methods and apparatus for performing digital assays using polydisperse droplets
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
AU2015250034B2 (en) 2014-04-21 2019-06-20 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN110333347A (zh) 2014-05-23 2019-10-15 萤火虫生物股份有限公司 用于生物测定的底物介导的反应器
CA2950043C (en) 2014-06-11 2022-08-02 Samplix S.A.R.L. Nucleotide sequence exclusion enrichment by droplet sorting (needls)
CN113249435B (zh) * 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
WO2015200717A2 (en) 2014-06-27 2015-12-30 The Regents Of The University Of California Pcr-activated sorting (pas)
WO2016064755A2 (en) 2014-10-20 2016-04-28 The Regents Of The University Of Califronia Rapid modulation of droplet composition with membrane microvalves
WO2016065056A1 (en) 2014-10-22 2016-04-28 The Regents Of The University Of California High definition microdroplet printer
WO2016126865A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Multiple-emulsion nucleic acid amplification
AU2016215304B2 (en) 2015-02-04 2022-01-27 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
EP3337907A4 (en) 2015-08-17 2019-01-02 The Regents of The University of California Microdroplet-based multiple displacement amplification (mda) methods and related compositions
JP2019515674A (ja) 2016-04-19 2019-06-13 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝的解析および他の適用のための固定化ベースのシステムおよび方法
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
CA3047328A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
EP3559272B1 (en) 2017-11-17 2022-08-31 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012109600A2 (en) * 2011-02-11 2012-08-16 Raindance Technologies, Inc. Methods for forming mixed droplets
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US20140272988A1 (en) * 2013-03-14 2014-09-18 Cold Spring Harbor Laboratory Trans-splicing transcriptome profiling
US20140323316A1 (en) * 2013-03-15 2014-10-30 Complete Genomics, Inc. Multiple tagging of individual long dna fragments

Cited By (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11203787B2 (en) 2012-08-13 2021-12-21 The Regents Of The University Of California Methods and systems for detecting biological components
US10745762B2 (en) 2012-08-13 2020-08-18 The Regents Of The University Of California Method and system for synthesizing a target polynucleotide within a droplet
US11001896B2 (en) 2012-08-13 2021-05-11 The Regents Of The University Of California System and method to synthesize a target molecule within a droplet
US11891666B2 (en) 2012-08-13 2024-02-06 The Regents Of The University Of California Methods and systems for detecting biological components
US11312990B2 (en) 2014-06-27 2022-04-26 The Regents Of The University Of California PCR-activated sorting (PAS)
US11020736B2 (en) 2014-10-22 2021-06-01 The Regents Of The University Of California High definition microdroplet printer
US11732287B2 (en) 2015-02-04 2023-08-22 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US12448643B2 (en) 2015-02-04 2025-10-21 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11111519B2 (en) 2015-02-04 2021-09-07 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
JP2018014924A (ja) * 2016-07-27 2018-02-01 国立大学法人京都大学 ヘリカーゼを用いたpcr
US11142791B2 (en) 2016-08-10 2021-10-12 The Regents Of The University Of California Combined multiple-displacement amplification and PCR in an emulsion microdroplet
US11124830B2 (en) 2016-12-21 2021-09-21 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US11781129B2 (en) 2017-10-18 2023-10-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
JP2021534956A (ja) * 2018-08-17 2021-12-16 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California 安定なジェットからの単分散粒子に誘発される液滴の形成
JP7504078B2 (ja) 2018-08-17 2024-06-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 安定なジェットからの単分散粒子に誘発される液滴の形成
JP2022522221A (ja) * 2019-04-02 2022-04-14 ミッション バイオ インコーポレイテッド 腫瘍を特性決定し、腫瘍の不均質性を識別するための方法及びシステム
US11365441B2 (en) 2019-05-22 2022-06-21 Mission Bio, Inc. Method and apparatus for simultaneous targeted sequencing of DNA, RNA and protein
US11667954B2 (en) 2019-07-01 2023-06-06 Mission Bio, Inc. Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2021241721A1 (ja) * 2020-05-29 2021-12-02 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JP7423101B2 (ja) 2020-05-29 2024-01-29 国立研究開発法人理化学研究所 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法
JPWO2021241721A1 (OSRAM) * 2020-05-29 2021-12-02

Also Published As

Publication number Publication date
EP3253479A2 (en) 2017-12-13
US20240150811A1 (en) 2024-05-09
US11111519B2 (en) 2021-09-07
EP3253479B1 (en) 2022-09-21
CN115011670A (zh) 2022-09-06
EP4112744A1 (en) 2023-01-04
EP3253479A4 (en) 2018-10-10
WO2016126871A3 (en) 2016-10-06
AU2016215304B2 (en) 2022-01-27
US11732287B2 (en) 2023-08-22
WO2016126871A2 (en) 2016-08-11
HK1248169A1 (en) 2018-10-12
US20180216160A1 (en) 2018-08-02
JP2022088360A (ja) 2022-06-14
CN107530654A (zh) 2018-01-02
AU2022202780A1 (en) 2022-05-19
CA2974306A1 (en) 2016-08-11
US12448643B2 (en) 2025-10-21
AU2016215304A1 (en) 2017-08-10
US20170009274A1 (en) 2017-01-12
EP4112744B1 (en) 2025-08-27

Similar Documents

Publication Publication Date Title
US12448643B2 (en) Sequencing of nucleic acids via barcoding in discrete entities
US11312990B2 (en) PCR-activated sorting (PAS)
US20220154248A1 (en) Combined multiple-displacement amplification and pcr in an emulsion microdroplet
AU2022203056A1 (en) Multiple-emulsion nucleic acid amplification
Zhang et al. Single-molecule DNA amplification and analysis using microfluidics
JP2020535951A (ja) 単分散エマルションを生成する方法
JP2021534956A (ja) 安定なジェットからの単分散粒子に誘発される液滴の形成
Liu et al. Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics
HK1248169B (en) Sequencing of nucleic acids via barcoding in discrete entities
HK1248284B (en) Multiple-emulsion nucleic acid amplification

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190129

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20190129

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20191217

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20200311

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20200617

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20201117

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210216

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20210413

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210514

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20211019

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220217

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20220217

C11 Written invitation by the commissioner to file amendments

Free format text: JAPANESE INTERMEDIATE CODE: C11

Effective date: 20220301

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220524

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20220613

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20220614

A912 Re-examination (zenchi) completed and case transferred to appeal board

Free format text: JAPANESE INTERMEDIATE CODE: A912

Effective date: 20220902

C211 Notice of termination of reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C211

Effective date: 20220906