JP2018139607A5 - - Google Patents

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JP2018139607A5
JP2018139607A5 JP2018090651A JP2018090651A JP2018139607A5 JP 2018139607 A5 JP2018139607 A5 JP 2018139607A5 JP 2018090651 A JP2018090651 A JP 2018090651A JP 2018090651 A JP2018090651 A JP 2018090651A JP 2018139607 A5 JP2018139607 A5 JP 2018139607A5
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target nucleic
amplification
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nucleic acid
nucleic acids
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JP2018090651A 2012-06-15 2018-05-09 核酸ライブラリーの動力学排除増幅 Active JP6585767B2 (ja)

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US201261660487P 2012-06-15 2012-06-15
US61/660,487 2012-06-15
US201261715478P 2012-10-18 2012-10-18
US61/715,478 2012-10-18
US13/783,043 2013-03-01
US13/783,043 US8895249B2 (en) 2012-06-15 2013-03-01 Kinetic exclusion amplification of nucleic acid libraries

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JP2018139607A5 true JP2018139607A5 (enExample) 2018-11-08
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EP (2) EP2861756B1 (enExample)
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KR (2) KR101757668B1 (enExample)
CN (2) CN104379766B (enExample)
AU (3) AU2013274283B2 (enExample)
BR (1) BR112014024897B1 (enExample)
CA (1) CA2867716C (enExample)
DK (2) DK2861756T3 (enExample)
ES (2) ES2662098T3 (enExample)
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Families Citing this family (205)

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Publication number Priority date Publication date Assignee Title
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
CN110079588B (zh) 2010-12-17 2024-03-15 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
WO2012106546A2 (en) 2011-02-02 2012-08-09 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
JP6193252B2 (ja) 2011-12-01 2017-09-06 ジナプシス インコーポレイテッド 高効率電子配列決定及び検出のためのシステム並びに方法
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
US9593365B2 (en) 2012-10-17 2017-03-14 Spatial Transcriptions Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US20160023208A1 (en) 2013-03-13 2016-01-28 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
EP3553175B1 (en) 2013-03-13 2021-07-14 Illumina, Inc. Method of preparing a nucleic acid sequencing library
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
DK3013983T3 (da) 2013-06-25 2023-03-06 Prognosys Biosciences Inc Spatialt kodede biologiske assays ved brug af en mikrofluidisk anordning
JP2016539343A (ja) 2013-08-30 2016-12-15 イルミナ インコーポレイテッド 親水性または斑状親水性表面上の液滴の操作
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
WO2015095291A1 (en) 2013-12-19 2015-06-25 Illumina, Inc. Substrates comprising nano-patterning surfaces and methods of preparing thereof
US10246746B2 (en) 2013-12-20 2019-04-02 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic DNA samples
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015161054A2 (en) * 2014-04-18 2015-10-22 Genapsys, Inc. Methods and systems for nucleic acid amplification
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
GB201416422D0 (en) * 2014-09-17 2014-10-29 Illumina Cambridge Ltd Flexible tape-based chemistry apparatus
CA2962941C (en) * 2014-09-29 2022-06-14 Illumina Cambridge Limited Recombinase mutants
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
WO2016075204A1 (en) 2014-11-11 2016-05-19 Illumina, Inc. Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
EP3234187B1 (en) 2014-12-15 2021-02-17 Illumina, Inc. Method for single molecular placement on a substrate
CN120425026A (zh) 2015-04-10 2025-08-05 十程基因技术瑞典公司 生物样本的空间区别、多重核酸分析
CN108449971B (zh) 2015-04-14 2020-10-16 亿明达股份有限公司 用于改进对光发射的检测的结构化基底及涉及其的方法
JP2018518157A (ja) 2015-05-11 2018-07-12 イラミーナ インコーポレーテッド 治療薬剤の発見および分析のためのプラットフォーム
JP6609641B2 (ja) * 2015-05-29 2019-11-20 イルミナ ケンブリッジ リミテッド クラスターにおける表面プライマーの高度な利用
JP6698708B2 (ja) 2015-06-09 2020-05-27 ライフ テクノロジーズ コーポレーション 分子タグ付けのための方法、システム、組成物、キット、装置、及びコンピュータ可読媒体
AU2016298541B2 (en) * 2015-07-30 2019-10-31 Illumina, Inc. Orthogonal deblocking of nucleotides
WO2017062343A1 (en) 2015-10-06 2017-04-13 Pierce Biotechnology, Inc. Devices and methods for producing nucleic acids and proteins
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
AU2017241670B2 (en) 2016-03-28 2020-10-08 Illumina, Inc. Recombinase mutants
PL3377226T3 (pl) 2016-03-28 2021-07-26 Illumina, Inc. Wielopłaszczyznowe mikromacierze
WO2017176896A1 (en) 2016-04-07 2017-10-12 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
JP6979554B2 (ja) * 2016-05-03 2021-12-15 ユニバーシティー ヘルス ネットワーク 無細胞メチル化dnaを捕捉する方法及びその使用
US10544456B2 (en) 2016-07-20 2020-01-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
KR102475710B1 (ko) 2016-07-22 2022-12-08 오레곤 헬스 앤드 사이언스 유니버시티 단일 세포 전체 게놈 라이브러리 및 이의 제조를 위한 조합 인덱싱 방법
GB201704754D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
US11896944B2 (en) 2017-02-01 2024-02-13 Illumina, Inc. System and method with fiducials responding to multiple excitation frequencies
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
US20200102587A1 (en) 2017-04-05 2020-04-02 Hitachi High-Technologies Corporation Nucleic acid amplification method and nucleic acid analyzer
ES2937929T3 (es) 2017-04-23 2023-04-03 Illumina Inc Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácido nucleico indexadas
AU2018259202B2 (en) 2017-04-23 2022-03-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
ES2893528T3 (es) 2017-04-23 2022-02-09 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en colecciones de ácidos nucleicos indexadas
US10975430B2 (en) 2017-04-23 2021-04-13 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US12584169B2 (en) 2017-04-23 2026-03-24 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN107254572B (zh) * 2017-06-01 2019-07-02 首钢集团有限公司 一种冷轧硅锰双相钢表面麻点缺陷的控制方法
KR102628035B1 (ko) * 2017-06-07 2024-01-22 오레곤 헬스 앤드 사이언스 유니버시티 메틸화 서열결정을 위한 단일 세포 전체 게놈 라이브러리
EP3662482A1 (en) 2017-07-31 2020-06-10 Illumina Inc. Sequencing system with multiplexed biological sample aggregation
FI3662083T3 (fi) 2017-08-01 2024-11-20 Illumina Inc Geneettisen materiaalin ja kirjastovalmisteen spatiaalinen indeksointi hydrogeelihelmiä ja virtauskennoja käyttämällä
EP3676010B1 (en) 2017-09-01 2024-07-03 MGI Tech Co., Ltd. An injection molded microfluidic/fluidic cartridge integrated with silicon-based sensor
CA3076378A1 (en) 2017-09-21 2019-03-28 Genapsys, Inc. Systems and methods for nucleic acid sequencing
CN107622185B (zh) * 2017-10-27 2020-08-21 领航基因科技(杭州)有限公司 一种数字pcr浓度计算方法
IL276343B2 (en) * 2018-01-29 2024-10-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
CN110832086A (zh) 2018-04-02 2020-02-21 伊鲁米那股份有限公司 用于制造用于基于序列的遗传检验的对照的组合物和方法
KR102640255B1 (ko) 2018-05-17 2024-02-27 일루미나, 인코포레이티드 감소된 증폭 편향을 갖는 고속-대량 단일 세포 서열분석
KR102507415B1 (ko) 2018-06-04 2023-03-07 일루미나, 인코포레이티드 고속 대량 단일 세포 전사체 라이브러리 그리고 이의 제조 및 사용 방법
CA3106410A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
EP4603594A3 (en) 2018-08-28 2025-11-12 10X Genomics, Inc. Increasing spatial array resolution
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US20220290217A1 (en) 2018-12-10 2022-09-15 10X Genomics, Inc. Resolving spatial arrays using deconvolution
JP7587425B2 (ja) * 2018-12-18 2024-11-20 イルミナ ケンブリッジ リミテッド 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物
BR112020026667A2 (pt) * 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3924515A4 (en) * 2019-02-11 2022-08-24 Ultima Genomics, Inc. PROCEDURE FOR NUCLEIC ACID ANALYSIS
US12624350B2 (en) 2019-03-01 2026-05-12 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US12409449B2 (en) 2019-08-01 2025-09-09 Illumina, Inc. Flow cells
CN110694599A (zh) * 2019-09-26 2020-01-17 浙江海洋大学 一种聚丙烯亚胺修饰的磁性纳晶纤维素分子印迹聚合物的制备方法
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
MX2021011847A (es) 2019-12-19 2021-11-17 Illumina Inc Genotecas de celulas unicas de alto rendimiento y metodos para elaborarlas y usarlas.
EP4081777B1 (en) 2019-12-23 2026-02-18 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
JP7525615B2 (ja) * 2020-01-17 2024-07-30 深▲せん▼華大智造科技有限公司 Dnaのセンス鎖とアンチセンス鎖のシーケンシングを同期させる方法
US11060138B1 (en) * 2020-01-17 2021-07-13 Element Biosciences, Inc. Nucleic acid sequencing systems
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
PL3969618T3 (pl) 2020-03-09 2024-01-03 Illumina, Inc. Sposoby sekwencjonowania polinukleotydów
CN114026250B (zh) 2020-03-09 2025-05-16 因美纳有限公司 核酸测序的改善
EP4127220B1 (en) 2020-03-30 2024-06-05 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
EP4150065A2 (en) 2020-05-12 2023-03-22 Illumina Inc Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
WO2021263111A1 (en) 2020-06-25 2021-12-30 10X Genomics, Inc. Spatial analysis of dna methylation
EP4172364A1 (en) 2020-06-30 2023-05-03 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
BR112023000073A2 (pt) 2020-07-08 2023-04-11 Illumina Inc Microesferas como transportadores de transpossomos
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
WO2022055729A1 (en) 2020-09-14 2022-03-17 Illumina, Inc. Compositions and methods for amplifying polynucleotides
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555B1 (en) 2020-12-21 2026-02-25 10X Genomics, Inc. Methods for capturing probes and/or barcodes
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
JP2024502618A (ja) 2021-01-08 2024-01-22 セラノーム, インコーポレイテッド 生体試料を分析するためのデバイスおよび方法
KR20230154303A (ko) * 2021-01-29 2023-11-07 일루미나, 인코포레이티드 폴리뉴클레오티드로 플로우 셀의 시딩 효율을 개선하기위한 방법, 조성물 및 키트
CA3210451A1 (en) 2021-03-22 2022-09-29 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
WO2022256503A1 (en) 2021-06-03 2022-12-08 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
CN117597453A (zh) 2021-06-23 2024-02-23 伊鲁米纳公司 用于测序试剂的组合物、方法、试剂盒、料筒和系统
AU2022296802A1 (en) 2021-06-25 2024-01-04 Illumina, Inc. Linear fourier fiducial
WO2022271689A1 (en) 2021-06-25 2022-12-29 Illumina, Inc. Fiducials for use in registration of a patterned surface
WO2023003757A1 (en) 2021-07-19 2023-01-26 Illumina Software, Inc. Intensity extraction with interpolation and adaptation for base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
GB2629259B (en) 2021-07-21 2025-05-28 Element Biosciences Inc Optical systems for nucleic acid sequencing and methods thereof
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4373965A1 (en) 2021-07-21 2024-05-29 DNAe Diagnostics Limited Method and system comprising a cartridge for sequencing target polynucleotides
WO2023004357A1 (en) * 2021-07-23 2023-01-26 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
CN118103750A (zh) 2021-08-31 2024-05-28 伊鲁米纳公司 具有增强的孔成像分辨率的流动池
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
EP4409028A2 (en) 2021-09-30 2024-08-07 Illumina, Inc. Blocker methods
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
WO2023114397A1 (en) 2021-12-16 2023-06-22 Illumina, Inc. Hybrid clustering
US20230212667A1 (en) 2021-12-29 2023-07-06 Illumina Cambridge Limited Methods of nucleic acid sequencing using surface-bound primers
WO2023175037A2 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
WO2023175026A1 (en) 2022-03-15 2023-09-21 Illumina, Inc. Methods of determining sequence information
CA3223090A1 (en) 2022-03-31 2023-10-05 Xiaoyu Ma Paired-end re-synthesis using blocked p5 primers
EP4504930A1 (en) 2022-04-07 2025-02-12 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20240033738A1 (en) 2022-07-27 2024-02-01 Illumina, Inc. Flow cell based motion system calibration and control methods
WO2024044705A1 (en) * 2022-08-26 2024-02-29 Illumina, Inc. Flow cells with patterned bonding regions
JP2025528992A (ja) 2022-09-19 2025-09-04 イルミナ インコーポレイテッド 固定化プライマーを含む変形可能なポリマー
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
WO2024072799A1 (en) 2022-09-26 2024-04-04 Illumina, Inc. Flow cell based motion system calibration and control methods
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
CN120153070A (zh) 2022-09-30 2025-06-13 因美纳有限公司 解旋酶-胞苷脱氨酶复合物及其使用方法
EP4594482A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240110234A1 (en) * 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
GB202215624D0 (en) 2022-10-21 2022-12-07 Dnae Diagnostics Ltd Clonal amplification
WO2024102809A1 (en) 2022-11-09 2024-05-16 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
KR20250130601A (ko) 2022-11-29 2025-09-02 엘리먼트 바이오사이언스, 인크. 핵산 시퀀싱을 위한 플로우 셀 디바이스 및 광학 시스템
CN119095978A (zh) 2022-12-07 2024-12-06 伊路米纳有限公司 使用具有模式化接种的双链dna大小排阻的单克隆聚簇
WO2024129969A1 (en) 2022-12-14 2024-06-20 Illumina, Inc. Systems and methods for capture and enrichment of clustered beads on flow cell substrates
EP4720334A1 (en) 2023-05-26 2026-04-08 Illumina, Inc. Methods for preserving methylation status during clustering
EP4720339A1 (en) 2023-05-31 2026-04-08 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2024249061A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Detecting a methylated nucleotide using a quencher
WO2024256581A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Determination of modified cytosines
EP4728091A1 (en) 2023-06-14 2026-04-22 Illumina, Inc. Concurrent sequencing with spatially separated rings
WO2025062002A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Concurrent sequencing using nick translation
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
WO2025072783A1 (en) 2023-09-28 2025-04-03 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025072683A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137479A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
WO2025207535A1 (en) 2024-03-26 2025-10-02 Illumina, Inc. Photo-switchable surfaces
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026006746A2 (en) 2024-06-28 2026-01-02 Illumina, Inc. Nucleic acid preparation and analysis techniques
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026097017A2 (en) 2024-11-01 2026-05-07 Illumina, Inc. Methods of improving sequencing accuracy

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US169513A (en) 1875-11-02 Improvement in electric telegraphs
US4787963A (en) 1987-05-04 1988-11-29 Syntro Corporation Method and means for annealing complementary nucleic acid molecules at an accelerated rate
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68926118T2 (de) 1988-08-18 1996-08-22 Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
DE69115690T2 (de) 1990-04-11 1996-05-30 Ludwig Inst Cancer Res Verfahren und gerät zum folgerichtigen chemischen reaktionsablauf
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5227265A (en) 1990-11-30 1993-07-13 Eastman Kodak Company Migration imaging system
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5728532A (en) * 1996-05-31 1998-03-17 Ackley; Donald E. Electrode configuration for matrix addressing of a molecular detection device
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
WO1998053296A1 (en) 1997-05-19 1998-11-26 Charles Evans And Associates Analysis of molecules bound to solid surfaces using selective bond cleavage processes
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6699693B1 (en) 1999-02-04 2004-03-02 Sloan-Kettering Institute For Cancer Research Process for DNA replication
WO2000046408A1 (en) 1999-02-04 2000-08-10 Sloan-Kettering Institute For Cancer Research Process for dna replication
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
CA2370976C (en) 1999-04-20 2009-10-20 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050191698A1 (en) 1999-04-20 2005-09-01 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
AU2001238068A1 (en) 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
ATE377093T1 (de) 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
DE60226856D1 (de) 2001-04-20 2008-07-10 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
CA2485596C (en) 2002-05-10 2013-04-16 City Of Hope Pyrophosphorolysis activated polymerization (pap)
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
DE60324810D1 (de) 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
EP1636337A4 (en) 2003-06-20 2007-07-04 Illumina Inc METHODS AND COMPOSITIONS USEFUL FOR THE AMPLIFICATION AND GENOTYPING OF THE GENOME
CA2531105C (en) 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
US20110059865A1 (en) 2004-01-07 2011-03-10 Mark Edward Brennan Smith Modified Molecular Arrays
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
AU2006259565B2 (en) 2005-06-15 2011-01-06 Complete Genomics, Inc. Single molecule arrays for genetic and chemical analysis
EP1929049B1 (en) 2005-07-25 2013-04-10 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
EP2112227B1 (en) * 2006-03-07 2013-05-15 Cargill, Incorporated Aldolases, nucleic acids encoding them and methods for making and using them
EP2021503A1 (en) 2006-03-17 2009-02-11 Solexa Ltd. Isothermal methods for creating clonal single molecule arrays
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US7702468B2 (en) * 2006-05-03 2010-04-20 Population Diagnostics, Inc. Evaluating genetic disorders
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック, インコーポレイテッド レコンビナーゼポリメラーゼ増幅
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
EP2639578B1 (en) 2006-12-14 2016-09-14 Life Technologies Corporation Apparatus for measuring analytes using large scale fet arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
BRPI0813718A2 (pt) 2007-07-13 2014-12-30 Univ Leland Stanford Junior Método e aparelho que utilizam um campo elétrico para ensaios biológicos aperfeiçoados
US8906831B2 (en) 2008-03-31 2014-12-09 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
WO2010037085A1 (en) * 2008-09-29 2010-04-01 The Board Of Trustees Of The University Of Illinois Dna sequencing and amplification systems using nanoscale field effect sensor arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
JP5815518B2 (ja) * 2009-07-17 2015-11-17 キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. マイクロ流体装置においてdnaを単離する方法及びシステム
US20130053252A1 (en) 2009-09-25 2013-02-28 President & Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
EP2539464B1 (en) 2010-02-23 2016-11-16 Illumina, Inc. Amplification methods to minimise sequence specific bias
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
EP2619333B1 (en) * 2010-09-23 2017-06-21 Centrillion Technology Holdings Corporation Native-extension parallel sequencing
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20130344540A1 (en) * 2011-02-03 2013-12-26 Mark T. Reed Methods for minimizing sequence specific bias
CN102212621B (zh) * 2011-05-09 2014-01-08 中国人民解放军第四军医大学 一种用于乙型肝炎病毒基因分型的检测试剂盒及检测方法
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA3003082C (en) 2011-10-28 2020-12-15 Illumina, Inc. Microarray fabrication system and method
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

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