JP2017504360A5 - - Google Patents
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- JP2017504360A5 JP2017504360A5 JP2016567462A JP2016567462A JP2017504360A5 JP 2017504360 A5 JP2017504360 A5 JP 2017504360A5 JP 2016567462 A JP2016567462 A JP 2016567462A JP 2016567462 A JP2016567462 A JP 2016567462A JP 2017504360 A5 JP2017504360 A5 JP 2017504360A5
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- Japan
- Prior art keywords
- sequence
- primer
- adapter
- target
- universal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091093088 Amplicon Proteins 0.000 claims 65
- 238000003752 polymerase chain reaction Methods 0.000 claims 42
- 238000000034 method Methods 0.000 claims 36
- 239000000758 substrate Substances 0.000 claims 28
- 230000000295 complement effect Effects 0.000 claims 26
- 108020004707 nucleic acids Proteins 0.000 claims 26
- 102000039446 nucleic acids Human genes 0.000 claims 26
- 150000007523 nucleic acids Chemical class 0.000 claims 26
- 238000000137 annealing Methods 0.000 claims 25
- 239000011541 reaction mixture Substances 0.000 claims 17
- 108010042407 Endonucleases Proteins 0.000 claims 12
- 102000004533 Endonucleases Human genes 0.000 claims 12
- 238000003776 cleavage reaction Methods 0.000 claims 10
- 230000007017 scission Effects 0.000 claims 10
- 108091033319 polynucleotide Proteins 0.000 claims 9
- 102000040430 polynucleotide Human genes 0.000 claims 9
- 239000002157 polynucleotide Substances 0.000 claims 9
- 102000004150 Flap endonucleases Human genes 0.000 claims 7
- 108090000652 Flap endonucleases Proteins 0.000 claims 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
- 238000007403 mPCR Methods 0.000 claims 5
- 102000003960 Ligases Human genes 0.000 claims 4
- 108090000364 Ligases Proteins 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 101710163270 Nuclease Proteins 0.000 claims 3
- 230000029087 digestion Effects 0.000 claims 3
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 claims 2
- MXHRCPNRJAMMIM-SHYZEUOFSA-N 2'-deoxyuridine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-SHYZEUOFSA-N 0.000 claims 2
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims 2
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 2
- 229930010555 Inosine Natural products 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims 2
- MXHRCPNRJAMMIM-UHFFFAOYSA-N desoxyuridine Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-UHFFFAOYSA-N 0.000 claims 2
- 229960003786 inosine Drugs 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010052833 ribonuclease HI Proteins 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461934515P | 2014-01-31 | 2014-01-31 | |
| US61/934,515 | 2014-01-31 | ||
| US201462078309P | 2014-11-11 | 2014-11-11 | |
| US201462078313P | 2014-11-11 | 2014-11-11 | |
| US62/078,309 | 2014-11-11 | ||
| US62/078,313 | 2014-11-11 | ||
| PCT/US2015/013994 WO2015117040A1 (en) | 2014-01-31 | 2015-01-30 | Improved methods for processing dna substrates |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018092887A Division JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017504360A JP2017504360A (ja) | 2017-02-09 |
| JP2017504360A5 true JP2017504360A5 (enExample) | 2018-04-26 |
| JP6723929B2 JP6723929B2 (ja) | 2020-07-15 |
Family
ID=53757794
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567462A Active JP6723929B2 (ja) | 2014-01-31 | 2015-01-30 | Ｄｎａ基質を処理するための改善された方法 |
| JP2018092887A Active JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018092887A Active JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US10316357B2 (enExample) |
| EP (5) | EP3099796B1 (enExample) |
| JP (2) | JP6723929B2 (enExample) |
| KR (2) | KR102321956B1 (enExample) |
| CN (2) | CN106459968B (enExample) |
| AU (1) | AU2015210705B2 (enExample) |
| CA (2) | CA2938213C (enExample) |
| DK (1) | DK3604544T3 (enExample) |
| ES (1) | ES2908644T3 (enExample) |
| HU (1) | HUE058149T2 (enExample) |
| WO (1) | WO2015117040A1 (enExample) |
Families Citing this family (87)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111254500B (zh) | 2012-12-10 | 2024-01-23 | 分析生物科学有限公司 | 靶向基因组分析的方法 |
| EP2986741B1 (en) | 2013-04-17 | 2019-07-24 | Agency For Science, Technology And Research | Method for generating extended sequence reads |
| CA2922089C (en) | 2013-08-22 | 2022-07-05 | E. I. Du Pont De Nemours And Company | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
| MX376900B (es) | 2013-12-11 | 2025-03-04 | Accuragen Holdings Ltd | Composiciones y metodos para detectar variantes poco frecuentes de una secuencia. |
| US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| EP3099796B1 (en) | 2014-01-31 | 2018-08-29 | Swift Biosciences, Inc. | Improved methods for processing dna substrates |
| EP3114231B1 (en) * | 2014-03-03 | 2019-01-02 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
| KR102425438B1 (ko) | 2014-06-23 | 2022-07-27 | 더 제너럴 하스피탈 코포레이션 | 서열결정에 의해 평가된 DSB의 게놈 전체에 걸친 비편향된 확인 (GUIDE-Seq) |
| US10619163B2 (en) * | 2014-07-03 | 2020-04-14 | Rhodx, Inc. | Tagging and assessing a target sequence |
| US20160053301A1 (en) | 2014-08-22 | 2016-02-25 | Clearfork Bioscience, Inc. | Methods for quantitative genetic analysis of cell free dna |
| EP3192869B1 (en) | 2014-09-12 | 2019-03-27 | MGI Tech Co., Ltd. | Isolated oligonucleotide and use thereof in nucleic acid sequencing |
| US10221448B2 (en) * | 2015-03-06 | 2019-03-05 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
| US10385387B2 (en) * | 2015-04-20 | 2019-08-20 | Pacific Biosciences Of California, Inc. | Methods for selectively amplifying and tagging nucleic acids |
| EP3347467B1 (en) | 2015-09-11 | 2021-06-23 | The General Hospital Corporation | Full interrogation of nuclease dsbs and sequencing (find-seq) |
| HK1259101A1 (zh) | 2015-10-09 | 2019-11-22 | Accuragen Holdings Limited | 用於富集扩增产物的方法及组合物 |
| AU2016353133B2 (en) | 2015-11-11 | 2022-12-08 | Resolution Bioscience, Inc. | High efficiency construction of dna libraries |
| CN106811460B (zh) * | 2015-11-30 | 2020-11-27 | 浙江安诺优达生物科技有限公司 | 用于低频突变检测的二代测序文库的构建方法及试剂盒 |
| EP3383994A4 (en) | 2015-12-03 | 2019-08-28 | Accuragen Holdings Limited | METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS |
| EP3387152B1 (en) | 2015-12-08 | 2022-01-26 | Twinstrand Biosciences, Inc. | Improved adapters, methods, and compositions for duplex sequencing |
| ES2924487T3 (es) * | 2016-01-29 | 2022-10-07 | Hoffmann La Roche | Un adaptador de conformación en Y novedoso para secuenciación de ácidos nucleicos y procedimiento de uso |
| EP3778918B1 (en) * | 2016-03-22 | 2022-03-09 | Vib Vzw | Means and methods for amplifying nucleotide sequences |
| CN109511265B (zh) | 2016-05-16 | 2023-07-14 | 安可济控股有限公司 | 通过链鉴定改进测序的方法 |
| EP3458614A4 (en) * | 2016-05-18 | 2020-01-22 | Cornell University | METHODS OF DETECTING A MUT GENE BY MULTIPLEX DIGITAL PCR |
| EP3910068A1 (en) * | 2016-05-24 | 2021-11-17 | The Translational Genomics Research Institute | Molecular tagging methods and sequencing libraries |
| US10370701B2 (en) | 2016-06-17 | 2019-08-06 | Pacific Biosciences Of California, Inc. | Methods and compositions for generating asymmetrically-tagged nucleic acid fragments |
| US11299780B2 (en) | 2016-07-15 | 2022-04-12 | The Regents Of The University Of California | Methods of producing nucleic acid libraries |
| WO2018035170A1 (en) | 2016-08-15 | 2018-02-22 | Accuragen Holdings Limited | Compositions and methods for detecting rare sequence variants |
| AU2017315769B2 (en) | 2016-08-25 | 2024-02-01 | Resolution Bioscience, Inc. | Methods for the detection of genomic copy changes in DNA samples |
| CN110139931B (zh) * | 2016-08-30 | 2024-06-11 | 元素生物科学公司 | 用于定相测序的方法和组合物 |
| CN109804083B (zh) * | 2016-09-22 | 2023-11-03 | 西格马-奥尔德里奇有限责任公司 | 单引物至双引物扩增子转换 |
| JP6980998B2 (ja) * | 2016-11-28 | 2021-12-15 | 東洋紡株式会社 | 核酸増幅用組成物 |
| US10982351B2 (en) | 2016-12-23 | 2021-04-20 | Grail, Inc. | Methods for high efficiency library preparation using double-stranded adapters |
| CN110248675B (zh) * | 2017-01-27 | 2024-01-09 | 合成Dna技术公司 | 利用竞争性链置换构建下一代测序(ngs)文库 |
| US10894979B2 (en) | 2017-01-27 | 2021-01-19 | Integrated Dna Technologies, Inc. | Construction of next generation sequencing (NGS) libraries using competitive strand displacement |
| JP6933907B2 (ja) * | 2017-02-24 | 2021-09-08 | シスメックス株式会社 | 核酸増幅方法 |
| US11479807B2 (en) * | 2017-03-23 | 2022-10-25 | University Of Washington | Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing |
| US10487317B1 (en) | 2017-03-23 | 2019-11-26 | New England Biolabs, Inc. | Stabilizing 5′-flap endonuclease activity |
| US10941445B2 (en) * | 2017-03-24 | 2021-03-09 | Bio-Rad Laboratories, Inc. | Universal hairpin primers |
| EP3601611B1 (en) * | 2017-03-24 | 2022-01-05 | Life Technologies Corporation | Polynucleotide adapters and methods of use thereof |
| US11274344B2 (en) | 2017-03-30 | 2022-03-15 | Grail, Inc. | Enhanced ligation in sequencing library preparation |
| WO2018183897A1 (en) | 2017-03-31 | 2018-10-04 | Grail, Inc. | Higher target capture efficiency using probe extension |
| WO2018232594A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | Pcr引物对及其应用 |
| US12104205B2 (en) * | 2017-06-20 | 2024-10-01 | Mgi Tech Co., Ltd. | PCR primer pair and application thereof |
| WO2018232595A1 (zh) * | 2017-06-20 | 2018-12-27 | 深圳华大智造科技有限公司 | Pcr引物对及其应用 |
| CN110612355B (zh) * | 2017-06-20 | 2024-01-12 | 深圳华大智造科技股份有限公司 | 用于定量pcr扩增的组合物及其应用 |
| CN107217308A (zh) * | 2017-06-21 | 2017-09-29 | 北京贝瑞和康生物技术股份有限公司 | 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒 |
| US20200131564A1 (en) * | 2017-07-07 | 2020-04-30 | Board Of Regents, The University Of Texas System | High-coverage and ultra-accurate immune repertoire sequencing using molecular identifiers |
| MX2020002002A (es) * | 2017-08-21 | 2020-07-20 | Taiho Pharmaceutical Co Ltd | Proteina de fusion de proteina dctn1 con proteina ret. |
| WO2019040650A1 (en) | 2017-08-23 | 2019-02-28 | The General Hospital Corporation | GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES HAVING MODIFIED PAM SPECIFICITY |
| US11725228B2 (en) | 2017-10-11 | 2023-08-15 | The General Hospital Corporation | Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies |
| US11739367B2 (en) | 2017-11-08 | 2023-08-29 | Twinstrand Biosciences, Inc. | Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters |
| WO2019140201A1 (en) | 2018-01-12 | 2019-07-18 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
| GB201802744D0 (en) * | 2018-02-20 | 2018-04-04 | Longas Tech Pty Ltd | Method |
| WO2019165168A1 (en) | 2018-02-23 | 2019-08-29 | Pioneer Hi-Bred International, Inc. | Novel cas9 orthologs |
| WO2019191900A1 (en) * | 2018-04-03 | 2019-10-10 | Burning Rock Biotech | Compositions and methods for preparing nucleic acid libraries |
| US11898203B2 (en) | 2018-04-17 | 2024-02-13 | The General Hospital Corporation | Highly sensitive in vitro assays to define substrate preferences and sites of nucleic-acid binding, modifying, and cleaving agents |
| CA3099065A1 (en) * | 2018-05-11 | 2019-11-14 | Pioneer Hi-Bred International, Inc. | Methods for the identification and characterization of double-strand break sites and compositions and uses thereof |
| AU2019280712B2 (en) | 2018-06-06 | 2025-11-20 | The Regents Of The University Of California | Methods of producing nucleic acid libraries and compositions and kits for practicing same |
| US12049665B2 (en) | 2018-06-12 | 2024-07-30 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
| CN112673099A (zh) | 2018-07-12 | 2021-04-16 | 特温斯特兰德生物科学有限公司 | 用于表征基因组编辑、克隆扩增的方法和试剂及相关应用 |
| EP3821032A1 (en) * | 2018-07-13 | 2021-05-19 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patient stratification |
| EP3730626A1 (en) * | 2019-04-24 | 2020-10-28 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patient stratification |
| EP3594364A1 (en) * | 2018-07-13 | 2020-01-15 | HiFiBio SAS High Fidelity Biology | Method of assaying nucleic acid in microfluidic droplets |
| JP2021531824A (ja) * | 2018-07-13 | 2021-11-25 | ハイファイバイオ エスアエス | 患者層別化のための液滴単一細胞エピゲノムプロファイリングの使用 |
| EP3730625A1 (en) * | 2019-04-24 | 2020-10-28 | HiFiBiO SAS | Use of droplet single cell epigenome profiling for patent stratification |
| DK3827011T3 (da) | 2018-07-24 | 2024-07-08 | Salish Bioscience Inc | Fremgangsmåder og sammensætning til målrettet genomisk analyse |
| BR112021011372A2 (pt) | 2018-12-14 | 2021-08-31 | Pioneer Hi-Bred International, Inc. | Novos sistemas crispr-cas para edição de genoma |
| GB201903391D0 (en) * | 2019-03-12 | 2019-04-24 | Cancer Research Tech Ltd | Nucleic acid amplification methods |
| WO2021027706A1 (zh) * | 2019-08-09 | 2021-02-18 | 深圳市真迈生物科技有限公司 | 捕获核酸分子的方法、核酸文库的制备方法及测序方法 |
| US12430567B2 (en) * | 2019-08-14 | 2025-09-30 | Microsoft Technology Licensing, Llc | Multiplex similarity search in DNA data storage |
| CN110565174B (zh) * | 2019-09-17 | 2022-09-30 | 北京博昊云天科技有限公司 | 一种dna文库构建方法 |
| EP4041877A1 (en) * | 2019-10-01 | 2022-08-17 | Universitetet i Tromsø - Norges Arktiske Universitet | Dna polymerase and dna polymerase derived 3'-5'exonuclease |
| GB2589869A (en) * | 2019-12-09 | 2021-06-16 | Univ Oxford Innovation Ltd | Method for whole genome sequencing of picogram quantities of DNA |
| WO2021178893A2 (en) * | 2020-03-06 | 2021-09-10 | Singular Genomics Systems, Inc. | Linked paired strand sequencing |
| CN116249775A (zh) | 2020-05-15 | 2023-06-09 | 合成Dna技术公司 | 连接偶联pcr的方法 |
| EP4211261B1 (en) * | 2020-09-11 | 2024-11-20 | Illumina Cambridge Limited | Methods of enriching a target sequence from a sequencing library using hairpin adaptors |
| JP2023553983A (ja) | 2020-12-11 | 2023-12-26 | ザ ブロード インスティテュート，インコーポレーテッド | 二重鎖シーケンシングのための方法 |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11220707B1 (en) | 2021-06-17 | 2022-01-11 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| KR20240099145A (ko) * | 2021-09-16 | 2024-06-28 | 세페이드 | 에코 증폭: 특정 핵산 서열의 증폭 및 검출을 위한 종합적인 화학 시스템 및 방법 |
| JP2025508067A (ja) * | 2022-03-09 | 2025-03-21 | ニユー・イングランド・バイオレイブス・インコーポレイテツド | ターゲット濃縮 |
| CN115928222A (zh) * | 2022-07-13 | 2023-04-07 | 翌圣生物科技(上海)股份有限公司 | 提升文库转化率的建库方法 |
| EP4623077A1 (en) * | 2022-11-21 | 2025-10-01 | Biosearch Technologies, Inc. | High-throughput amplification of targeted nucleic acid sequences |
| TW202526033A (zh) * | 2023-08-30 | 2025-07-01 | 南韓商西諾海利斯股份有限公司 | 使用限制酶製備ｒｎａ文庫的方法 |
| WO2025235889A1 (en) | 2024-05-10 | 2025-11-13 | Guardant Health, Inc. | Methods involving multiplexed pooled pcr |
| WO2026005608A1 (en) | 2024-06-28 | 2026-01-02 | Koninklijke Nederlandse Akademie Van Wetenschappen | Adapters and methods for multiplexing cell-free DNA |
Family Cites Families (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL331513A1 (en) * | 1996-06-06 | 1999-07-19 | Lynx Therapeutics | Method of sequencing, by a ligand effect, specific encoded adapters and composition containing double-string oligonucleotidic adapters |
| AUPO188396A0 (en) * | 1996-08-26 | 1996-09-19 | Insearch Limited | Detection of protozoan parasites |
| US20020058250A1 (en) * | 1997-03-21 | 2002-05-16 | Marshall, Gerstein & Borun | Extraction and utilisation of vntr alleles |
| US5888737A (en) | 1997-04-15 | 1999-03-30 | Lynx Therapeutics, Inc. | Adaptor-based sequence analysis |
| US6287825B1 (en) * | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
| US6958225B2 (en) * | 1999-10-27 | 2005-10-25 | Affymetrix, Inc. | Complexity management of genomic DNA |
| GB0308851D0 (en) * | 2003-04-16 | 2003-05-21 | Lingvitae As | Method |
| EP1924704B1 (en) * | 2005-08-02 | 2011-05-25 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| GB0522310D0 (en) * | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20070172839A1 (en) * | 2006-01-24 | 2007-07-26 | Smith Douglas R | Asymmetrical adapters and methods of use thereof |
| WO2008033442A2 (en) * | 2006-09-12 | 2008-03-20 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for performing low background multiplex nucleic acid amplification reactions |
| WO2008049021A2 (en) * | 2006-10-17 | 2008-04-24 | Life Technologies Corporation | Methods of allele typing |
| WO2012044847A1 (en) * | 2010-10-01 | 2012-04-05 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| US20110003701A1 (en) | 2008-02-27 | 2011-01-06 | 454 Life Sciences Corporation | System and method for improved processing of nucleic acids for production of sequencable libraries |
| US20090291475A1 (en) | 2008-04-23 | 2009-11-26 | Kai Qin Lao | Sequence amplification with linear primers |
| WO2009133466A2 (en) * | 2008-04-30 | 2009-11-05 | Population Genetics Technologies Ltd. | Asymmetric adapter library construction |
| US8586310B2 (en) * | 2008-09-05 | 2013-11-19 | Washington University | Method for multiplexed nucleic acid patch polymerase chain reaction |
| JP6147502B2 (ja) | 2009-10-27 | 2017-06-14 | スウィフト バイオサイエンシーズ， インコーポレイテッド | ポリヌクレオチドプライマー及びプローブ |
| US9023769B2 (en) | 2009-11-30 | 2015-05-05 | Complete Genomics, Inc. | cDNA library for nucleic acid sequencing |
| US8574832B2 (en) * | 2010-02-03 | 2013-11-05 | Massachusetts Institute Of Technology | Methods for preparing sequencing libraries |
| US20120238738A1 (en) | 2010-07-19 | 2012-09-20 | New England Biolabs, Inc. | Oligonucleotide Adapters: Compositions and Methods of Use |
| US20120077716A1 (en) | 2010-09-29 | 2012-03-29 | 454 Life Sciences Corporation | System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine |
| CN102061335B (zh) * | 2010-11-15 | 2014-07-23 | 苏州众信生物技术有限公司 | 一种二代高通量测序的不对称dna双链接头及其应用 |
| WO2012112582A2 (en) * | 2011-02-14 | 2012-08-23 | Swift Biosciences, Inc. | Polynucleotide primers and probes |
| WO2012149154A1 (en) * | 2011-04-26 | 2012-11-01 | Swift Biosciences, Inc. | Polynucleotide primers and probes |
| EP3495497B1 (en) * | 2011-04-28 | 2021-03-24 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| EP2714938B1 (en) | 2011-05-27 | 2017-11-15 | President and Fellows of Harvard College | Methods of amplifying whole genome of a single cell |
| WO2013081864A1 (en) * | 2011-11-29 | 2013-06-06 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| EP2966180B1 (en) | 2011-11-29 | 2017-08-16 | Life Technologies Corporation | Methods and compositions for multiplex pcr |
| CA2862552A1 (en) * | 2012-01-26 | 2013-08-01 | Nugen Technologies, Inc. | Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation |
| JP6509723B2 (ja) | 2012-03-13 | 2019-05-08 | スウィフト バイオサイエンシーズ， インコーポレイテッド | 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物 |
| EP2855707B1 (en) * | 2012-05-31 | 2017-07-12 | Board Of Regents, The University Of Texas System | Method for accurate sequencing of dna |
| CN102776172B (zh) * | 2012-06-13 | 2013-08-07 | 山东农业大学 | 一种通用多重pcr方法 |
| EP3071711A1 (en) | 2013-11-18 | 2016-09-28 | Rubicon Genomics | Degradable adaptors for background reduction |
| EP3099796B1 (en) | 2014-01-31 | 2018-08-29 | Swift Biosciences, Inc. | Improved methods for processing dna substrates |
| WO2015120177A1 (en) * | 2014-02-07 | 2015-08-13 | Qiagen Sciences Llc | Pcr primers |
| EP3114231B1 (en) * | 2014-03-03 | 2019-01-02 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
| EP3192869B1 (en) * | 2014-09-12 | 2019-03-27 | MGI Tech Co., Ltd. | Isolated oligonucleotide and use thereof in nucleic acid sequencing |
| US10221448B2 (en) * | 2015-03-06 | 2019-03-05 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
| WO2016144619A1 (en) * | 2015-03-06 | 2016-09-15 | Pillar Biosciences Inc. | Selective amplification of overlapping amplicons |
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2015
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