JP2017504360A5 - - Google Patents
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- JP2017504360A5 JP2017504360A5 JP2016567462A JP2016567462A JP2017504360A5 JP 2017504360 A5 JP2017504360 A5 JP 2017504360A5 JP 2016567462 A JP2016567462 A JP 2016567462A JP 2016567462 A JP2016567462 A JP 2016567462A JP 2017504360 A5 JP2017504360 A5 JP 2017504360A5
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- Japan
- Prior art keywords
- sequence
- primer
- adapter
- target
- universal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091093088 Amplicon Proteins 0.000 claims 65
- 238000003752 polymerase chain reaction Methods 0.000 claims 42
- 238000000034 method Methods 0.000 claims 36
- 239000000758 substrate Substances 0.000 claims 28
- 230000000295 complement effect Effects 0.000 claims 26
- 108020004707 nucleic acids Proteins 0.000 claims 26
- 102000039446 nucleic acids Human genes 0.000 claims 26
- 150000007523 nucleic acids Chemical class 0.000 claims 26
- 238000000137 annealing Methods 0.000 claims 25
- 239000011541 reaction mixture Substances 0.000 claims 17
- 108010042407 Endonucleases Proteins 0.000 claims 12
- 102000004533 Endonucleases Human genes 0.000 claims 12
- 238000003776 cleavage reaction Methods 0.000 claims 10
- 230000007017 scission Effects 0.000 claims 10
- 108091033319 polynucleotide Proteins 0.000 claims 9
- 102000040430 polynucleotide Human genes 0.000 claims 9
- 239000002157 polynucleotide Substances 0.000 claims 9
- 102000004150 Flap endonucleases Human genes 0.000 claims 7
- 108090000652 Flap endonucleases Proteins 0.000 claims 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 6
- 238000007403 mPCR Methods 0.000 claims 5
- 102000003960 Ligases Human genes 0.000 claims 4
- 108090000364 Ligases Proteins 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 101710163270 Nuclease Proteins 0.000 claims 3
- 230000029087 digestion Effects 0.000 claims 3
- 238000012986 modification Methods 0.000 claims 3
- 230000004048 modification Effects 0.000 claims 3
- VGONTNSXDCQUGY-RRKCRQDMSA-N 2'-deoxyinosine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(N=CNC2=O)=C2N=C1 VGONTNSXDCQUGY-RRKCRQDMSA-N 0.000 claims 2
- MXHRCPNRJAMMIM-SHYZEUOFSA-N 2'-deoxyuridine Chemical group C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-SHYZEUOFSA-N 0.000 claims 2
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims 2
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 2
- 229930010555 Inosine Natural products 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims 2
- MXHRCPNRJAMMIM-UHFFFAOYSA-N desoxyuridine Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-UHFFFAOYSA-N 0.000 claims 2
- 229960003786 inosine Drugs 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 238000012163 sequencing technique Methods 0.000 claims 2
- 108010083644 Ribonucleases Proteins 0.000 claims 1
- 102000006382 Ribonucleases Human genes 0.000 claims 1
- 108010052833 ribonuclease HI Proteins 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461934515P | 2014-01-31 | 2014-01-31 | |
| US61/934,515 | 2014-01-31 | ||
| US201462078313P | 2014-11-11 | 2014-11-11 | |
| US201462078309P | 2014-11-11 | 2014-11-11 | |
| US62/078,309 | 2014-11-11 | ||
| US62/078,313 | 2014-11-11 | ||
| PCT/US2015/013994 WO2015117040A1 (en) | 2014-01-31 | 2015-01-30 | Improved methods for processing dna substrates |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018092887A Division JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017504360A JP2017504360A (ja) | 2017-02-09 |
| JP2017504360A5 true JP2017504360A5 (enExample) | 2018-04-26 |
| JP6723929B2 JP6723929B2 (ja) | 2020-07-15 |
Family
ID=53757794
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016567462A Active JP6723929B2 (ja) | 2014-01-31 | 2015-01-30 | Ｄｎａ基質を処理するための改善された方法 |
| JP2018092887A Active JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018092887A Active JP6633684B2 (ja) | 2014-01-31 | 2018-05-14 | Ｄｎａ基質を処理するための改善された方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US10316357B2 (enExample) |
| EP (5) | EP3604544B1 (enExample) |
| JP (2) | JP6723929B2 (enExample) |
| KR (2) | KR102482592B1 (enExample) |
| CN (2) | CN109971826B (enExample) |
| AU (1) | AU2015210705B2 (enExample) |
| CA (2) | CA2938213C (enExample) |
| DK (1) | DK3604544T3 (enExample) |
| ES (1) | ES2908644T3 (enExample) |
| HU (1) | HUE058149T2 (enExample) |
| WO (1) | WO2015117040A1 (enExample) |
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2015
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