DK3604544T3 - Improved methods for processing dna substrates - Google Patents

Improved methods for processing dna substrates Download PDF

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Publication number
DK3604544T3
DK3604544T3 DK19197131.6T DK19197131T DK3604544T3 DK 3604544 T3 DK3604544 T3 DK 3604544T3 DK 19197131 T DK19197131 T DK 19197131T DK 3604544 T3 DK3604544 T3 DK 3604544T3
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improved methods
dna substrates
processing dna
processing
substrates
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DK19197131.6T
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English (en)
Inventor
Vladimir Makarov
Julie Laliberte
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Swift Biosciences Inc
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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DK19197131.6T 2014-01-31 2015-01-30 Improved methods for processing dna substrates DK3604544T3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461934515P 2014-01-31 2014-01-31
US201462078313P 2014-11-11 2014-11-11
US201462078309P 2014-11-11 2014-11-11
EP18165783.4A EP3363904B1 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

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DK3604544T3 true DK3604544T3 (en) 2022-02-14

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US (5) US10316357B2 (da)
EP (5) EP4273264A3 (da)
JP (2) JP6723929B2 (da)
KR (2) KR102321956B1 (da)
CN (2) CN106459968B (da)
AU (1) AU2015210705B2 (da)
CA (2) CA3211550A1 (da)
DK (1) DK3604544T3 (da)
ES (1) ES2908644T3 (da)
HU (1) HUE058149T2 (da)
WO (1) WO2015117040A1 (da)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201508193TA (en) 2013-04-17 2015-11-27 Agency Science Tech & Res Method for generating extended sequence reads
CN109706222A (zh) 2013-12-11 2019-05-03 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
AU2015210705B2 (en) 2014-01-31 2020-11-05 Integrated Dna Technologies, Inc. Improved methods for processing DNA substrates
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
US10619163B2 (en) * 2014-07-03 2020-04-14 Rhodx, Inc. Tagging and assessing a target sequence
AU2014406026B2 (en) 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
US10221448B2 (en) * 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10385387B2 (en) * 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
JP2018530536A (ja) * 2015-09-11 2018-10-18 ザ ジェネラル ホスピタル コーポレイション ヌクレアーゼＤＳＢの完全照合およびシーケンシング（ＦＩＮＤ−ｓｅｑ）
CN114807323A (zh) 2015-10-09 2022-07-29 安可济控股有限公司 用于富集扩增产物的方法及组合物
CN108431233B (zh) * 2015-11-11 2022-04-01 分析生物科学有限公司 Dna文库的高效率构建
CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
PL3387152T3 (pl) 2015-12-08 2022-05-09 Twinstrand Biosciences, Inc. Ulepszone adaptory, sposoby i kompozycje do sekwencjonowania dupleksowego
JP6714709B2 (ja) * 2016-01-29 2020-06-24 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 核酸配列決定のための新規アダプターおよび使用法
US10752943B2 (en) * 2016-03-22 2020-08-25 Vib Vzw Means and methods for amplifying nucleotide sequences
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
EP3458614A4 (en) * 2016-05-18 2020-01-22 Cornell University METHOD FOR DETECTING A MUTED GENE BY MULTIPLEXING DIGITAL PCR
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US11299780B2 (en) 2016-07-15 2022-04-12 The Regents Of The University Of California Methods of producing nucleic acid libraries
SG10202111825YA (en) 2016-08-15 2021-12-30 Accuragen Holdings Ltd Compositions and methods for detecting rare sequence variants
BR112019003704A2 (pt) 2016-08-25 2019-05-28 Resolution Bioscience Inc métodos para a detecção de alterações na cópia genômica em amostras de dna
EP3507365A4 (en) * 2016-08-30 2020-05-06 Metabiotech Corporation METHOD AND COMPOSITIONS FOR PHASE CONTROLLED SEQUENCING
CN109804083B (zh) 2016-09-22 2023-11-03 西格马-奥尔德里奇有限责任公司 单引物至双引物扩增子转换
JP6980998B2 (ja) * 2016-11-28 2021-12-15 東洋紡株式会社 核酸増幅用組成物
EP3559255A1 (en) 2016-12-23 2019-10-30 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
HUE059100T2 (hu) * 2017-01-27 2022-10-28 Integrated Dna Tech Inc Következõ generációs szekvenálási (NGS) könyvtárak létrehozása kompetitív szálhelyettesítéssel
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
US10487317B1 (en) 2017-03-23 2019-11-26 New England Biolabs, Inc. Stabilizing 5′-flap endonuclease activity
JP7256748B2 (ja) 2017-03-23 2023-04-12 ユニヴァーシティ オブ ワシントン エラーが訂正された核酸配列決定への適用を伴う標的化核酸配列濃縮のための方法
CN110446787A (zh) * 2017-03-24 2019-11-12 生物辐射实验室股份有限公司 通用发夹引物
US20200377935A1 (en) * 2017-03-24 2020-12-03 Life Technologies Corporation Polynucleotide adapters and methods of use thereof
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
WO2018232597A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 用于定量pcr扩增的组合物及其应用
EP3643788A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PAIR OF PRIMERS FOR PCR AND RELATED APPLICATION
EP3643787A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PCR PRIMER PAIR AND USE OF IT
WO2018232598A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 Pcr引物对及其应用
CN107217308A (zh) * 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
WO2019010486A1 (en) * 2017-07-07 2019-01-10 Board Of Regents, The University Of Texas System ULTRA-ACCURATE AND HIGH COVERAGE OF IMMUNE REPERTOIRE USING MOLECULAR IDENTIFIERS
SG11202001212SA (en) * 2017-08-21 2020-03-30 Taiho Pharmaceutical Co Ltd Fusion protein of dctn1 protein with ret protein
US11286468B2 (en) 2017-08-23 2022-03-29 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases with altered PAM specificity
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
EP3707273B1 (en) 2017-11-08 2024-04-10 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
AU2019207900A1 (en) * 2018-01-12 2020-07-09 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB201802744D0 (en) * 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
CN112313241A (zh) 2018-04-17 2021-02-02 总医院公司 核酸结合、修饰、和切割试剂的底物偏好和位点的灵敏体外试验
WO2019217816A1 (en) * 2018-05-11 2019-11-14 Pioneer Hi-Bred International, Inc. Methods for the identification and characterization of double-strand break sites and compositions and uses thereof
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
JP2021530219A (ja) 2018-07-12 2021-11-11 ツインストランド・バイオサイエンシズ・インコーポレイテッドTwinstrand Biosciences, Inc. ゲノム編集、クローン増殖、および関連用途を特徴付けるための方法および試薬
EP3730625A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patent stratification
EP3730626A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
EP3594364A1 (en) * 2018-07-13 2020-01-15 HiFiBio SAS High Fidelity Biology Method of assaying nucleic acid in microfluidic droplets
CN112996924A (zh) * 2018-07-13 2021-06-18 高保真生物技术有限公司 液滴单细胞表观基因组谱分析用于患者分层的用途
WO2020012004A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
CA3107052A1 (en) * 2018-07-24 2020-01-30 Ripple Biosolutions Llc Methods and composition for targeted genomic analysis
KR20210104068A (ko) 2018-12-14 2021-08-24 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 게놈 편집을 위한 신규한 crispr-cas 시스템
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods
WO2021027706A1 (zh) * 2019-08-09 2021-02-18 深圳市真迈生物科技有限公司 捕获核酸分子的方法、核酸文库的制备方法及测序方法
US20210050073A1 (en) * 2019-08-14 2021-02-18 Microsoft Technology Licensing, Llc Multiplex similarity search in dna data storage
CN110565174B (zh) * 2019-09-17 2022-09-30 北京博昊云天科技有限公司 一种dna文库构建方法
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
AU2022346850A1 (en) * 2021-09-16 2024-03-28 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences
WO2023172934A1 (en) * 2022-03-09 2023-09-14 New England Biolabs, Inc. Target enrichment

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4124377B2 (ja) * 1996-06-06 2008-07-23 ソレクサ・インコーポレイテッド コードアダプターの連結による配列決定
AUPO188396A0 (en) * 1996-08-26 1996-09-19 Insearch Limited Detection of protozoan parasites
KR20010005544A (ko) * 1997-03-21 2001-01-15 그레그 펄쓰 Ｖｎｔｒ 대립 유전자의 추출 및 이용방법
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
DK1924704T3 (da) * 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2008033442A2 (en) * 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
JP2010506595A (ja) * 2006-10-17 2010-03-04 ライフ テクノロジーズ コーポレーション 対立遺伝子タイピングの方法
US9540637B2 (en) * 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
US20110003701A1 (en) 2008-02-27 2011-01-06 454 Life Sciences Corporation System and method for improved processing of nucleic acids for production of sequencable libraries
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8586310B2 (en) * 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20110129832A1 (en) 2009-10-27 2011-06-02 Swift Biosciences, Inc. Polynucleotide Primers and Probes
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
CN102061335B (zh) * 2010-11-15 2014-07-23 苏州众信生物技术有限公司 一种二代高通量测序的不对称dna双链接头及其应用
SG192736A1 (en) * 2011-02-14 2013-09-30 Swift Biosciences Inc Polynucleotide primers and probes
US20140186844A1 (en) * 2011-04-26 2014-07-03 Swift Biosciences, Inc. Polynucleotide primers and probes
WO2012149438A1 (en) * 2011-04-28 2012-11-01 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2012166425A2 (en) 2011-05-27 2012-12-06 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
WO2013081864A1 (en) * 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
EP2785860B1 (en) 2011-11-29 2015-06-03 Life Technologies Corporation Methods and compositions for multiplex pcr
GB2533882B (en) * 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
WO2013181170A1 (en) * 2012-05-31 2013-12-05 Board Of Regents, The University Of Texas System Method for accurate sequencing of dna
CN102776172B (zh) * 2012-06-13 2013-08-07 山东农业大学 一种通用多重pcr方法
CN105917002A (zh) 2013-11-18 2016-08-31 鲁比康基因组学公司 用于减少背景的可降解适体
AU2015210705B2 (en) 2014-01-31 2020-11-05 Integrated Dna Technologies, Inc. Improved methods for processing DNA substrates
WO2015120177A1 (en) * 2014-02-07 2015-08-13 Qiagen Sciences Llc Pcr primers
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
US10221448B2 (en) * 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
ES2718765T3 (es) * 2015-03-06 2019-07-04 Pillar Biosciences Inc Amplificación selectiva de amplicones solapados

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