DK3604544T3 - Improved methods for processing dna substrates - Google Patents

Improved methods for processing dna substrates Download PDF

Info

Publication number
DK3604544T3
DK3604544T3 DK19197131.6T DK19197131T DK3604544T3 DK 3604544 T3 DK3604544 T3 DK 3604544T3 DK 19197131 T DK19197131 T DK 19197131T DK 3604544 T3 DK3604544 T3 DK 3604544T3
Authority
DK
Denmark
Prior art keywords
improved methods
dna substrates
processing dna
processing
substrates
Prior art date
Application number
DK19197131.6T
Other languages
Danish (da)
English (en)
Inventor
Vladimir Makarov
Julie Laliberte
Original Assignee
Swift Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757794&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3604544(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Swift Biosciences Inc filed Critical Swift Biosciences Inc
Application granted granted Critical
Publication of DK3604544T3 publication Critical patent/DK3604544T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/525—Phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
DK19197131.6T 2014-01-31 2015-01-30 Improved methods for processing dna substrates DK3604544T3 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201461934515P 2014-01-31 2014-01-31
US201462078309P 2014-11-11 2014-11-11
US201462078313P 2014-11-11 2014-11-11
EP18165783.4A EP3363904B1 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

Publications (1)

Publication Number Publication Date
DK3604544T3 true DK3604544T3 (en) 2022-02-14

Family

ID=53757794

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19197131.6T DK3604544T3 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

Country Status (11)

Country Link
US (6) US10316357B2 (enExample)
EP (5) EP3363904B1 (enExample)
JP (2) JP6723929B2 (enExample)
KR (2) KR102321956B1 (enExample)
CN (2) CN109971826B (enExample)
AU (1) AU2015210705B2 (enExample)
CA (2) CA2938213C (enExample)
DK (1) DK3604544T3 (enExample)
ES (1) ES2908644T3 (enExample)
HU (1) HUE058149T2 (enExample)
WO (1) WO2015117040A1 (enExample)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105531375B (zh) 2012-12-10 2020-03-03 分析生物科学有限公司 靶向基因组分析的方法
SG10201708498VA (en) 2013-04-17 2017-11-29 Agency Science Tech & Res Method for generating extended sequence reads
CN106133138A (zh) 2013-08-22 2016-11-16 先锋国际良种公司 使用引导多核苷酸/cas内切核酸酶系统的基因组修饰及其使用方法
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
IL274464B2 (en) 2013-12-11 2023-08-01 Accuragen Holdings Ltd Preparations and methods for the detection of rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
HUE058149T2 (hu) 2014-01-31 2022-07-28 Swift Biosciences Inc Javított eljárások DNS-szubsztrátok feldolgozására
US10208338B2 (en) * 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
AU2015280069B2 (en) 2014-06-23 2021-08-12 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
EP3164489B1 (en) * 2014-07-03 2020-05-13 Rhodx, Inc. Tagging and assessing a target sequence
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
WO2016037358A1 (zh) 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10385387B2 (en) * 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
CA3000816A1 (en) 2015-09-11 2017-03-16 The General Hospital Corporation Full interrogation of nuclease dsbs and sequencing (find-seq)
CN114807323A (zh) * 2015-10-09 2022-07-29 安可济控股有限公司 用于富集扩增产物的方法及组合物
EP3889257A1 (en) * 2015-11-11 2021-10-06 Resolution Bioscience, Inc. High efficiency construction of dna libraries
CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
WO2017100441A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
EP3408406B1 (en) * 2016-01-29 2022-06-15 F. Hoffmann-La Roche AG A novel y-shaped adaptor for nucleic acid sequencing and method of use
WO2017162754A1 (en) * 2016-03-22 2017-09-28 Vib Vzw Means and methods for amplifying nucleotide sequences
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
US20190144950A1 (en) * 2016-05-18 2019-05-16 Cornell University Methods of detecting a mutated gene by multiplex digital pcr
ES2870626T3 (es) * 2016-05-24 2021-10-27 Translational Genomics Res Inst Métodos de marcado molecular y bibliotecas de secuenciación
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
CN109563543B (zh) 2016-07-15 2022-08-30 加利福尼亚大学董事会 产生核酸库的方法
WO2018035170A1 (en) 2016-08-15 2018-02-22 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
KR102505122B1 (ko) 2016-08-25 2023-03-08 레졸루션 바이오사이언스, 인크. Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법
CN110139931B (zh) * 2016-08-30 2024-06-11 元素生物科学公司 用于定相测序的方法和组合物
US11390913B2 (en) 2016-09-22 2022-07-19 Sigma-Aldrich Co. Llc Single primer to dual primer amplicon switching
JP6980998B2 (ja) * 2016-11-28 2021-12-15 東洋紡株式会社 核酸増幅用組成物
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
JP6982087B2 (ja) * 2017-01-27 2021-12-17 インテグレイテッド ディエヌエイ テクノロジイズ, インコーポレイテッドIntegrated Dna Technologies, Incorporated 競合的鎖置換を利用する次世代シーケンシング（ｎｇｓ）ライブラリーの構築
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
CN118638898A (zh) * 2017-03-23 2024-09-13 华盛顿大学 用于靶向核酸序列富集的方法及在错误纠正的核酸测序中的应用
US10487317B1 (en) 2017-03-23 2019-11-26 New England Biolabs, Inc. Stabilizing 5′-flap endonuclease activity
US20200377935A1 (en) * 2017-03-24 2020-12-03 Life Technologies Corporation Polynucleotide adapters and methods of use thereof
WO2018175399A1 (en) * 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
WO2018183918A1 (en) 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
US11118222B2 (en) 2017-03-31 2021-09-14 Grail, Inc. Higher target capture efficiency using probe extension
WO2018232597A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 用于定量pcr扩增的组合物及其应用
CN110506114B (zh) * 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
US11739319B2 (en) * 2017-06-20 2023-08-29 Mgi Tech Co., Ltd. PCR primer pair and application thereof
US12104205B2 (en) * 2017-06-20 2024-10-01 Mgi Tech Co., Ltd. PCR primer pair and application thereof
CN107217308A (zh) * 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
WO2019010486A1 (en) * 2017-07-07 2019-01-10 Board Of Regents, The University Of Texas System ULTRA-ACCURATE AND HIGH COVERAGE OF IMMUNE REPERTOIRE USING MOLECULAR IDENTIFIERS
EP3674325A4 (en) * 2017-08-21 2021-11-03 Taiho Pharmaceutical Co., Ltd. FUSION PROTEIN OF DCTN1 PROTEIN WITH RET PROTEIN
WO2019040650A1 (en) 2017-08-23 2019-02-28 The General Hospital Corporation GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES HAVING MODIFIED PAM SPECIFICITY
WO2019075197A1 (en) 2017-10-11 2019-04-18 The General Hospital Corporation METHODS OF DETECTION OF INDIVIDUAL SITE-SPECIFIC PARASITE GENOMIC DEAMINATION BY BASE EDITING TECHNOLOGIES
CA3081027A1 (en) 2017-11-08 2019-05-16 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
US11584929B2 (en) 2018-01-12 2023-02-21 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
GB201802744D0 (en) 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
KR20200124702A (ko) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
WO2019191900A1 (en) * 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
AU2019256287B2 (en) 2018-04-17 2026-02-05 The General Hospital Corporation Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents
WO2019217816A1 (en) * 2018-05-11 2019-11-14 Pioneer Hi-Bred International, Inc. Methods for the identification and characterization of double-strand break sites and compositions and uses thereof
WO2019236726A1 (en) 2018-06-06 2019-12-12 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
CN112601823A (zh) 2018-06-12 2021-04-02 安可济控股有限公司 用于形成连接产物的方法和组合物
AU2019300172A1 (en) 2018-07-12 2021-01-28 Twinstrand Biosciences, Inc. Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
EP3730625A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patent stratification
EP3821032B1 (en) * 2018-07-13 2026-03-11 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
EP3594364A1 (en) * 2018-07-13 2020-01-15 HiFiBio SAS High Fidelity Biology Method of assaying nucleic acid in microfluidic droplets
EP3730626A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
CA3106307A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
EP3827011B1 (en) * 2018-07-24 2024-06-19 Salish Bioscience Inc. Methods and composition for targeted genomic analysis
SG11202101405RA (en) 2018-08-14 2021-03-30 Harvard College In vitro detection of nucleic acid
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods
EP4012029B1 (en) * 2019-08-09 2024-05-29 GeneMind Biosciences Company Limited Method for capturing nucleic acid molecule, preparation method for nucleic acid library, and a sequencing method
US12430567B2 (en) * 2019-08-14 2025-09-30 Microsoft Technology Licensing, Llc Multiplex similarity search in DNA data storage
CN110565174B (zh) * 2019-09-17 2022-09-30 北京博昊云天科技有限公司 一种dna文库构建方法
CA3155624A1 (en) 2019-10-01 2021-04-08 Universitetet I Tromso - Norges Arktiske Universitet Dna polymerase and dna polymerase derived 3'-5'exonuclease
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
WO2021178893A2 (en) * 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
WO2021232023A2 (en) 2020-05-15 2021-11-18 Swift Biosciences, Inc. Methods for ligation-coupled-pcr
CA3188197A1 (en) 2020-09-11 2022-03-17 Andrew Slatter Methods of enriching a target sequence from a sequencing library using hairpin adaptors
EP4259820A4 (en) * 2020-12-11 2024-12-11 The Broad Institute Inc. DUPLEX SEQUENCING PROCESS
US20240384318A1 (en) * 2021-06-14 2024-11-21 Primrose Bio, Inc. Compositions and methods for enzymatic nucleic acid synthesis
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11220707B1 (en) 2021-06-17 2022-01-11 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US20250146060A1 (en) * 2021-09-16 2025-05-08 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences
US20250179568A1 (en) * 2022-03-09 2025-06-05 New England Biolabs, Inc. Target Enrichment
CN115928222A (zh) * 2022-07-13 2023-04-07 翌圣生物科技(上海)股份有限公司 提升文库转化率的建库方法
CA3264896A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS
CN120548357A (zh) * 2022-11-21 2025-08-26 百奥赛科生物科技有限公司 目标核酸序列的高通量扩增
WO2025048512A1 (ko) * 2023-08-30 2025-03-06 주식회사 제노헬릭스 제한효소를 이용한 rna 라이브러리 제작 방법
WO2025235889A1 (en) 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr
NL2038081B1 (en) * 2024-06-28 2026-01-15 Koninklijke Nederlandse Akademie Van Wetenschappen Adapters and methods for multiplexing cell-free DNA
WO2026010830A1 (en) * 2024-07-01 2026-01-08 Watchmaker Genomics, Inc. Methods of polymerase free dna end repair
WO2026058704A1 (ja) * 2024-09-10 2026-03-19 国立大学法人 東京大学 Ｄｎａバーコード配列を含む一本鎖 ｄｎａの合成方法（ｍｅｔｈｏｄ ｆｏｒ ｓｙｎｔｈｅｓｉｚｉｎｇ ｓｉｎｇｌｅ－ｓｔｒａｎｄｅｄ ｄｎａ ｃｏｎｔａｉｎｉｎｇ ｄｎａ ｂａｒｃｏｄｅ ｓｅｑｕｅｎｃｅｓ）

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046704A1 (en) 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
AUPO188396A0 (en) * 1996-08-26 1996-09-19 Insearch Limited Detection of protozoan parasites
CA2283651A1 (en) * 1997-03-21 1998-10-01 Greg Firth Extraction and utilisation of vntr alleles
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
DK1924704T3 (da) * 2005-08-02 2011-09-05 Rubicon Genomics Inc Sammensætninger og fremgangsmåder til bearbejdning og mangfoldiggørelse af DNA, herunder ved anvendelse af flere enzymer i en enkelt reaktion
GB0522310D0 (en) * 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2008033442A2 (en) * 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
WO2008049021A2 (en) * 2006-10-17 2008-04-24 Life Technologies Corporation Methods of allele typing
US9540637B2 (en) * 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
US20110003701A1 (en) 2008-02-27 2011-01-06 454 Life Sciences Corporation System and method for improved processing of nucleic acids for production of sequencable libraries
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
WO2009133466A2 (en) * 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8586310B2 (en) * 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
WO2011056687A2 (en) 2009-10-27 2011-05-12 Swift Biosciences, Inc. Polynucleotide primers and probes
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
CN102061335B (zh) * 2010-11-15 2014-07-23 苏州众信生物技术有限公司 一种二代高通量测序的不对称dna双链接头及其应用
AU2012217788A1 (en) * 2011-02-14 2013-08-29 Swift Biosciences, Inc. Polynucleotide primers and probes
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes
WO2013081755A1 (en) 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
US9957558B2 (en) * 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
CN103890191B (zh) 2011-05-27 2018-12-04 哈佛大学校长及研究员协会 单细胞全基因组扩增方法
WO2013081864A1 (en) * 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
CN105861487B (zh) * 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
LT2825672T (lt) 2012-03-13 2019-06-10 Swift Biosciences, Inc. Būdai ir kompozicijos, skirti valdomam pagal dydį substrato polinukleotidų homopolimeriniam uodegų formavimui su nukleorūgščių polimeraze
AU2013267609C1 (en) * 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
CN102776172B (zh) * 2012-06-13 2013-08-07 山东农业大学 一种通用多重pcr方法
CN105917002A (zh) 2013-11-18 2016-08-31 鲁比康基因组学公司 用于减少背景的可降解适体
HUE058149T2 (hu) 2014-01-31 2022-07-28 Swift Biosciences Inc Javított eljárások DNS-szubsztrátok feldolgozására
WO2015120177A1 (en) * 2014-02-07 2015-08-13 Qiagen Sciences Llc Pcr primers
US10208338B2 (en) * 2014-03-03 2019-02-19 Swift Biosciences, Inc. Enhanced adaptor ligation
WO2016037358A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
US10221448B2 (en) * 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
AU2016229372B2 (en) * 2015-03-06 2020-01-30 Pillar Biosciences Inc. Selective amplification of overlapping amplicons

Also Published As

Publication number Publication date
US11162135B2 (en) 2021-11-02
US20210238657A1 (en) 2021-08-05
CN106459968A (zh) 2017-02-22
EP3099796A4 (en) 2017-08-23
JP6633684B2 (ja) 2020-01-22
EP3099796B1 (en) 2018-08-29
EP3604544A1 (en) 2020-02-05
KR20160115938A (ko) 2016-10-06
CA2938213A1 (en) 2015-08-06
JP6723929B2 (ja) 2020-07-15
WO2015117040A1 (en) 2015-08-06
EP3099796A1 (en) 2016-12-07
US10316357B2 (en) 2019-06-11
US20160362726A1 (en) 2016-12-15
US20190241935A1 (en) 2019-08-08
EP4039811A1 (en) 2022-08-10
EP4039811B1 (en) 2023-09-20
KR102482592B1 (ko) 2022-12-30
KR102321956B1 (ko) 2021-11-08
CN109971826A (zh) 2019-07-05
EP3604544B1 (en) 2021-12-15
US20220162672A1 (en) 2022-05-26
JP2018138052A (ja) 2018-09-06
CN106459968B (zh) 2020-02-21
AU2015210705B2 (en) 2020-11-05
AU2015210705A1 (en) 2016-08-11
CA2938213C (en) 2023-10-10
EP3363904B1 (en) 2019-10-23
US11203781B2 (en) 2021-12-21
CN109971826B (zh) 2022-12-30
EP3363904A3 (en) 2018-09-12
JP2017504360A (ja) 2017-02-09
HUE058149T2 (hu) 2022-07-28
EP3363904A2 (en) 2018-08-22
US20160340746A1 (en) 2016-11-24
ES2908644T3 (es) 2022-05-03
US20240360500A1 (en) 2024-10-31
CA3211550A1 (en) 2015-08-06
EP4273264A3 (en) 2024-01-17
US10316359B2 (en) 2019-06-11
KR20210135343A (ko) 2021-11-12
EP4273264A2 (en) 2023-11-08

Similar Documents

Publication Publication Date Title
HUE058149T2 (hu) Javított eljárások DNS-szubsztrátok feldolgozására
GB201413701D0 (en) Process
GB201417076D0 (en) Process
SG10201502813TA (en) Substrate Processing Apparatus
GB201405800D0 (en) Process
GB201405210D0 (en) Process
GB201408841D0 (en) Nucleic acid processing
GB201504782D0 (en) Process
GB201415862D0 (en) Process
GB201404468D0 (en) Process
GB201413340D0 (en) Process
GB201402950D0 (en) Process
GB2524136B (en) Process
GB201420285D0 (en) Process
GB201409126D0 (en) Process
GB201400137D0 (en) Process
SG10201502817UA (en) Substrate Processing Apparatus
GB201412406D0 (en) Process
GB201420111D0 (en) Process
PT3201364T (pt) Método para processar peles.
SG10201502295PA (en) Substrate processing method
GB201414292D0 (en) Process
GB201411627D0 (en) Process
GB201616917D0 (en) Process
GB201415972D0 (en) Process