AU2015210705B2 - Improved methods for processing DNA substrates - Google Patents

Improved methods for processing DNA substrates Download PDF

Info

Publication number
AU2015210705B2
AU2015210705B2 AU2015210705A AU2015210705A AU2015210705B2 AU 2015210705 B2 AU2015210705 B2 AU 2015210705B2 AU 2015210705 A AU2015210705 A AU 2015210705A AU 2015210705 A AU2015210705 A AU 2015210705A AU 2015210705 B2 AU2015210705 B2 AU 2015210705B2
Authority
AU
Australia
Prior art keywords
dna
adapter
oligonucleotide
ligation
primer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2015210705A
Other languages
English (en)
Other versions
AU2015210705A1 (en
Inventor
Julie LALIBERTE
Vladimir Makarov
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Integrated DNA Technologies Inc
Original Assignee
Integrated DNA Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53757794&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2015210705(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Integrated DNA Technologies Inc filed Critical Integrated DNA Technologies Inc
Publication of AU2015210705A1 publication Critical patent/AU2015210705A1/en
Application granted granted Critical
Publication of AU2015210705B2 publication Critical patent/AU2015210705B2/en
Assigned to INTEGRATED DNA TECHNOLOGIES, INC. reassignment INTEGRATED DNA TECHNOLOGIES, INC. Request for Assignment Assignors: SWIFT BIOSCIENCES, INC.
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855Ligating adaptors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/10Nucleotidyl transfering
    • C12Q2521/101DNA polymerase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/501Ligase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/525Phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00Reaction characterised by the enzymatic activity
    • C12Q2521/50Other enzymatic activities
    • C12Q2521/531Glycosylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/113Modifications characterised by incorporating modified backbone
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/186Modifications characterised by incorporating a non-extendable or blocking moiety
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/191Modifications characterised by incorporating an adaptor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • Plant Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2015210705A 2014-01-31 2015-01-30 Improved methods for processing DNA substrates Active AU2015210705B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461934515P 2014-01-31 2014-01-31
US61/934,515 2014-01-31
US201462078313P 2014-11-11 2014-11-11
US201462078309P 2014-11-11 2014-11-11
US62/078,309 2014-11-11
US62/078,313 2014-11-11
PCT/US2015/013994 WO2015117040A1 (en) 2014-01-31 2015-01-30 Improved methods for processing dna substrates

Publications (2)

Publication Number Publication Date
AU2015210705A1 AU2015210705A1 (en) 2016-08-11
AU2015210705B2 true AU2015210705B2 (en) 2020-11-05

Family

ID=53757794

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2015210705A Active AU2015210705B2 (en) 2014-01-31 2015-01-30 Improved methods for processing DNA substrates

Country Status (11)

Country Link
US (6) US10316357B2 (enExample)
EP (5) EP3604544B1 (enExample)
JP (2) JP6723929B2 (enExample)
KR (2) KR102482592B1 (enExample)
CN (2) CN109971826B (enExample)
AU (1) AU2015210705B2 (enExample)
CA (2) CA2938213C (enExample)
DK (1) DK3604544T3 (enExample)
ES (1) ES2908644T3 (enExample)
HU (1) HUE058149T2 (enExample)
WO (1) WO2015117040A1 (enExample)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HK1213944A1 (zh) 2012-12-10 2016-07-15 Resolution Bioscience, Inc. 用於靶向基因组分析
US11859171B2 (en) 2013-04-17 2024-01-02 Agency For Science, Technology And Research Method for generating extended sequence reads
MX363842B (es) 2013-08-22 2019-04-05 Du Pont Métodos para producir modificaciones genéticas en un genoma vegetal sin incorporar un marcador transgénico seleccionable, y composiciones de éstas.
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3080298B1 (en) 2013-12-11 2018-10-31 AccuraGen Holdings Limited Methods for detecting rare sequence variants
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
KR102482592B1 (ko) 2014-01-31 2022-12-30 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. Dna 기질을 처리하는 개선 방법
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
KR102425438B1 (ko) 2014-06-23 2022-07-27 더 제너럴 하스피탈 코포레이션 서열결정에 의해 평가된 DSB의 게놈 전체에 걸친 비편향된 확인 (GUIDE-Seq)
WO2016004368A2 (en) * 2014-07-03 2016-01-07 Rhodx, Inc. Tagging and assessing a target sequence
US20160053301A1 (en) 2014-08-22 2016-02-25 Clearfork Bioscience, Inc. Methods for quantitative genetic analysis of cell free dna
AU2014406026B2 (en) 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
US10221448B2 (en) * 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10385387B2 (en) * 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
WO2017044843A1 (en) 2015-09-11 2017-03-16 The General Hospital Corporation Full interrogation of nuclease dsbs and sequencing (find-seq)
HK1259101A1 (zh) * 2015-10-09 2019-11-22 Accuragen Holdings Limited 用於富集扩增产物的方法及组合物
JP6913089B2 (ja) * 2015-11-11 2021-08-04 レゾリューション バイオサイエンス, インコーポレイテッド Dnaライブラリーの高効率構築
CN106811460B (zh) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 用于低频突变检测的二代测序文库的构建方法及试剂盒
EP3383994A4 (en) 2015-12-03 2019-08-28 Accuragen Holdings Limited METHOD AND COMPOSITIONS FOR FORMING LIGATION PRODUCTS
PT3387152T (pt) 2015-12-08 2022-04-19 Twinstrand Biosciences Inc Adaptadores, métodos e composições melhorados para sequenciamento duplex
JP6714709B2 (ja) * 2016-01-29 2020-06-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 核酸配列決定のための新規アダプターおよび使用法
EP3433378B1 (en) * 2016-03-22 2020-09-02 Vib Vzw Means and methods for amplifying nucleotide sequences
EP3458586B1 (en) 2016-05-16 2022-12-28 Accuragen Holdings Limited Method of improved sequencing by strand identification
US20190144950A1 (en) * 2016-05-18 2019-05-16 Cornell University Methods of detecting a mutated gene by multiplex digital pcr
EP3910068B1 (en) * 2016-05-24 2026-01-28 The Translational Genomics Research Institute Molecular tagging methods and sequencing libraries
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
CN109563543B (zh) 2016-07-15 2022-08-30 加利福尼亚大学董事会 产生核酸库的方法
JP6966052B2 (ja) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド 稀な配列変異体を検出するための組成物および方法
KR102850460B1 (ko) 2016-08-25 2025-08-27 레졸루션 바이오사이언스, 인크. Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법
WO2018045109A1 (en) * 2016-08-30 2018-03-08 Metabiotech Corporation Methods and compositions for phased sequencing
CN117512078A (zh) * 2016-09-22 2024-02-06 西格马-奥尔德里奇有限责任公司 单引物至双引物扩增子转换
JP6980998B2 (ja) * 2016-11-28 2021-12-15 東洋紡株式会社 核酸増幅用組成物
WO2018119399A1 (en) * 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
EP3573646B1 (en) * 2017-01-27 2022-02-23 Integrated DNA Technologies, Inc. Construction of next generation sequencing (ngs) libraries using competitive strand displacement
JP6933907B2 (ja) * 2017-02-24 2021-09-08 シスメックス株式会社 核酸増幅方法
US10487317B1 (en) 2017-03-23 2019-11-26 New England Biolabs, Inc. Stabilizing 5′-flap endonuclease activity
ES2929281T3 (es) 2017-03-23 2022-11-28 Univ Washington Métodos para el enriquecimiento de secuencias de ácidos nucleicos dirigidos con aplicaciones para la secuenciación de ácidos nucleicos con corrección de errores
US10941445B2 (en) * 2017-03-24 2021-03-09 Bio-Rad Laboratories, Inc. Universal hairpin primers
EP4053294A1 (en) * 2017-03-24 2022-09-07 Life Technologies Corporation Polynucleotide adapters and methods of use thereof
WO2018183918A1 (en) 2017-03-30 2018-10-04 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183897A1 (en) 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
WO2018232597A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 用于定量pcr扩增的组合物及其应用
US12104205B2 (en) * 2017-06-20 2024-10-01 Mgi Tech Co., Ltd. PCR primer pair and application thereof
US11739319B2 (en) * 2017-06-20 2023-08-29 Mgi Tech Co., Ltd. PCR primer pair and application thereof
CN110506114B (zh) * 2017-06-20 2023-12-15 深圳华大智造科技股份有限公司 Pcr引物对及其应用
CN107217308A (zh) 2017-06-21 2017-09-29 北京贝瑞和康生物技术股份有限公司 一种用于检测染色体拷贝数变异的测序文库构建方法和试剂盒
US20200131564A1 (en) * 2017-07-07 2020-04-30 Board Of Regents, The University Of Texas System High-coverage and ultra-accurate immune repertoire sequencing using molecular identifiers
AU2018322286B2 (en) * 2017-08-21 2022-03-10 Taiho Pharmaceutical Co., Ltd. Fusion protein of DCTN1 protein with RET protein
EP3672612A4 (en) 2017-08-23 2021-09-29 The General Hospital Corporation GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES WITH MODIFIED PAM SPECIFICITY
US11725228B2 (en) 2017-10-11 2023-08-15 The General Hospital Corporation Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies
EP3707273B1 (en) 2017-11-08 2024-04-10 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
JP7296969B2 (ja) * 2018-01-12 2023-06-23 クラレット バイオサイエンス, エルエルシー 核酸を解析するための方法および組成物
GB201802744D0 (en) * 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
WO2019165168A1 (en) 2018-02-23 2019-08-29 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
WO2019191900A1 (en) * 2018-04-03 2019-10-10 Burning Rock Biotech Compositions and methods for preparing nucleic acid libraries
WO2019204378A1 (en) 2018-04-17 2019-10-24 The General Hospital Corporation Sensitive in vitro assays for substrate preferences and sites of nucleic acid binding, modifying, and cleaving agents
CA3099065A1 (en) * 2018-05-11 2019-11-14 Pioneer Hi-Bred International, Inc. Methods for the identification and characterization of double-strand break sites and compositions and uses thereof
EP3802864A1 (en) 2018-06-06 2021-04-14 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
KR20210059694A (ko) 2018-07-12 2021-05-25 트윈스트랜드 바이오사이언시스, 인코포레이티드 게놈 편집, 클론 팽창 및 연관된 분야를 규명하기 위한 방법 및 시약
CA3106307A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
EP3594364A1 (en) * 2018-07-13 2020-01-15 HiFiBio SAS High Fidelity Biology Method of assaying nucleic acid in microfluidic droplets
WO2020011998A1 (en) * 2018-07-13 2020-01-16 Hifibio Sas Use of droplet single cell epigenome profiling for patient stratification
EP3730625A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patent stratification
EP3730626A1 (en) * 2019-04-24 2020-10-28 HiFiBiO SAS Use of droplet single cell epigenome profiling for patient stratification
PL3827011T3 (pl) 2018-07-24 2024-10-14 Salish Bioscience Inc. Sposoby i kompozycje do celowanej analizy genomowej
US12365888B2 (en) 2018-12-14 2025-07-22 Pioneer Hi-Bred International, Inc. CRISPR-Cas systems for genome editing
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods
EP4012029B1 (en) * 2019-08-09 2024-05-29 GeneMind Biosciences Company Limited Method for capturing nucleic acid molecule, preparation method for nucleic acid library, and a sequencing method
US12430567B2 (en) * 2019-08-14 2025-09-30 Microsoft Technology Licensing, Llc Multiplex similarity search in DNA data storage
CN110565174B (zh) * 2019-09-17 2022-09-30 北京博昊云天科技有限公司 一种dna文库构建方法
EP4041877A1 (en) * 2019-10-01 2022-08-17 Universitetet i Tromsø - Norges Arktiske Universitet Dna polymerase and dna polymerase derived 3'-5'exonuclease
GB2589869A (en) * 2019-12-09 2021-06-16 Univ Oxford Innovation Ltd Method for whole genome sequencing of picogram quantities of DNA
WO2021178893A2 (en) * 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
AU2021270596A1 (en) * 2020-05-15 2022-12-15 Integrated Dna Technologies, Inc. Methods for ligation-coupled-PCR
MX2023001400A (es) * 2020-09-11 2023-04-25 Illumina Cambridge Ltd Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla.
WO2022125997A1 (en) 2020-12-11 2022-06-16 The Broad Institute, Inc. Method for duplex sequencing
US11236388B1 (en) 2021-06-17 2022-02-01 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2023043951A2 (en) * 2021-09-16 2023-03-23 Cepheid Echo amplification: a comprehensive system of chemistry and methods for amplification and detection of specific nucleic acid sequences
CN119095979A (zh) * 2022-03-09 2024-12-06 新英格兰生物实验室公司 目标富集
CN115928222A (zh) * 2022-07-13 2023-04-07 翌圣生物科技(上海)股份有限公司 提升文库转化率的建库方法
EP4573213A1 (en) 2022-08-15 2025-06-25 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids
AU2023385733A1 (en) * 2022-11-21 2025-06-12 Biosearch Technologies, Inc. High-throughput amplification of targeted nucleic acid sequences
TW202526033A (zh) * 2023-08-30 2025-07-01 南韓商西諾海利斯股份有限公司 使用限制酶製備rna文庫的方法
WO2025235889A1 (en) 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr
NL2038081B1 (en) 2024-06-28 2026-01-15 Koninklijke Nederlandse Akademie Van Wetenschappen Adapters and methods for multiplexing cell-free DNA
WO2026010830A1 (en) * 2024-07-01 2026-01-08 Watchmaker Genomics, Inc. Methods of polymerase free dna end repair

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046704A1 (en) * 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPO188396A0 (en) * 1996-08-26 1996-09-19 Insearch Limited Detection of protozoan parasites
CN1257551A (zh) * 1997-03-21 2000-06-21 格雷格·弗思 Vntr等位基因的提取与利用
US5888737A (en) 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
US6287825B1 (en) * 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US6958225B2 (en) * 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
GB0308851D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
US20070031857A1 (en) * 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
US20070172839A1 (en) * 2006-01-24 2007-07-26 Smith Douglas R Asymmetrical adapters and methods of use thereof
WO2008033442A2 (en) * 2006-09-12 2008-03-20 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for performing low background multiplex nucleic acid amplification reactions
EP2082062A4 (en) * 2006-10-17 2010-07-07 Life Technologies Corp EXPERIENCE FOR ALLELY TYPING
WO2012044847A1 (en) * 2010-10-01 2012-04-05 Life Technologies Corporation Nucleic acid adaptors and uses thereof
US20110003701A1 (en) 2008-02-27 2011-01-06 454 Life Sciences Corporation System and method for improved processing of nucleic acids for production of sequencable libraries
US20090291475A1 (en) 2008-04-23 2009-11-26 Kai Qin Lao Sequence amplification with linear primers
US8029993B2 (en) * 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8586310B2 (en) * 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
JP6147502B2 (ja) 2009-10-27 2017-06-14 スウィフト バイオサイエンシーズ, インコーポレイテッド ポリヌクレオチドプライマー及びプローブ
US9023769B2 (en) 2009-11-30 2015-05-05 Complete Genomics, Inc. cDNA library for nucleic acid sequencing
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
US20120238738A1 (en) 2010-07-19 2012-09-20 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
US20120077716A1 (en) 2010-09-29 2012-03-29 454 Life Sciences Corporation System and method for producing functionally distinct nucleic acid library ends through use of deoxyinosine
CN102061335B (zh) * 2010-11-15 2014-07-23 苏州众信生物技术有限公司 一种二代高通量测序的不对称dna双链接头及其应用
CA2826904A1 (en) * 2011-02-14 2012-08-23 Swift Biosciences, Inc. Polynucleotide primers and probes
WO2012149154A1 (en) * 2011-04-26 2012-11-01 Swift Biosciences, Inc. Polynucleotide primers and probes
EP2702170B1 (en) * 2011-04-28 2015-12-16 Life Technologies Corporation Methods and compositions for multiplex pcr
US9617598B2 (en) 2011-05-27 2017-04-11 President And Fellows Of Harvard College Methods of amplifying whole genome of a single cell
EP2966180B1 (en) 2011-11-29 2017-08-16 Life Technologies Corporation Methods and compositions for multiplex pcr
WO2013081864A1 (en) * 2011-11-29 2013-06-06 Life Technologies Corporation Methods and compositions for multiplex pcr
SG10201504490QA (en) * 2012-01-26 2015-07-30 Nugen Technologies Inc Compositions And Methods For Targeted Nucleic Acid Sequence Enrichment And High Efficiency Library Generation
EP2825672B1 (en) 2012-03-13 2019-02-13 Swift Biosciences, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
CN102776172B (zh) * 2012-06-13 2013-08-07 山东农业大学 一种通用多重pcr方法
US20160257985A1 (en) 2013-11-18 2016-09-08 Rubicon Genomics, Inc. Degradable adaptors for background reduction
KR102482592B1 (ko) 2014-01-31 2022-12-30 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. Dna 기질을 처리하는 개선 방법
EP3102699B1 (en) * 2014-02-07 2018-10-31 Qiagen Sciences, LLC Pcr primers
WO2015134552A1 (en) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Enhanced adaptor ligation
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
US10221448B2 (en) * 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
SG11201707154SA (en) * 2015-03-06 2017-09-28 Pillar Biosciences Inc Selective amplification of overlapping amplicons

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046704A1 (en) * 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors

Also Published As

Publication number Publication date
US20160340746A1 (en) 2016-11-24
KR102321956B1 (ko) 2021-11-08
KR102482592B1 (ko) 2022-12-30
EP3363904A3 (en) 2018-09-12
EP3099796B1 (en) 2018-08-29
US20240360500A1 (en) 2024-10-31
EP3363904B1 (en) 2019-10-23
US10316359B2 (en) 2019-06-11
CN109971826A (zh) 2019-07-05
US20220162672A1 (en) 2022-05-26
JP6633684B2 (ja) 2020-01-22
CN109971826B (zh) 2022-12-30
JP2017504360A (ja) 2017-02-09
WO2015117040A1 (en) 2015-08-06
HUE058149T2 (hu) 2022-07-28
EP4039811A1 (en) 2022-08-10
CA3211550A1 (en) 2015-08-06
EP3604544B1 (en) 2021-12-15
JP6723929B2 (ja) 2020-07-15
CA2938213A1 (en) 2015-08-06
EP4273264A2 (en) 2023-11-08
US10316357B2 (en) 2019-06-11
US20160362726A1 (en) 2016-12-15
US11162135B2 (en) 2021-11-02
ES2908644T3 (es) 2022-05-03
CA2938213C (en) 2023-10-10
EP4273264A3 (en) 2024-01-17
US11203781B2 (en) 2021-12-21
EP3099796A1 (en) 2016-12-07
EP3363904A2 (en) 2018-08-22
JP2018138052A (ja) 2018-09-06
US20210238657A1 (en) 2021-08-05
KR20160115938A (ko) 2016-10-06
CN106459968B (zh) 2020-02-21
EP3099796A4 (en) 2017-08-23
US20190241935A1 (en) 2019-08-08
DK3604544T3 (en) 2022-02-14
AU2015210705A1 (en) 2016-08-11
CN106459968A (zh) 2017-02-22
EP3604544A1 (en) 2020-02-05
KR20210135343A (ko) 2021-11-12
EP4039811B1 (en) 2023-09-20

Similar Documents

Publication Publication Date Title
US20240360500A1 (en) Methods for multiplex pcr
EP2966180B1 (en) Methods and compositions for multiplex pcr
US20170247756A1 (en) Genetic sequence verification compositions, methods and kits
HK40105106A (en) Improved methods for processing dna substrates
HK1261416A1 (en) Improved methods for processing dna substrates
HK1261416B (en) Improved methods for processing dna substrates
HK40023084B (en) Improved methods for processing dna substrates
HK40023084A (en) Improved methods for processing dna substrates
HK40011033B (en) Improved methods for processing dna substrates
HK1234775B (zh) 用於加工dna底物的改進方法
HK40011033A (zh) 用於加工dna底物的改進方法
HK1234775A1 (en) Improved methods for processing dna substrates

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: INTEGRATED DNA TECHNOLOGIES, INC.

Free format text: FORMER OWNER(S): SWIFT BIOSCIENCES, INC.