AU2015210705B2 - Improved methods for processing DNA substrates - Google Patents
Improved methods for processing DNA substrates Download PDFInfo
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- AU2015210705B2 AU2015210705B2 AU2015210705A AU2015210705A AU2015210705B2 AU 2015210705 B2 AU2015210705 B2 AU 2015210705B2 AU 2015210705 A AU2015210705 A AU 2015210705A AU 2015210705 A AU2015210705 A AU 2015210705A AU 2015210705 B2 AU2015210705 B2 AU 2015210705B2
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/501—Ligase
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/525—Phosphatase
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- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/531—Glycosylase
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/113—Modifications characterised by incorporating modified backbone
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461934515P | 2014-01-31 | 2014-01-31 | |
| US61/934,515 | 2014-01-31 | ||
| US201462078313P | 2014-11-11 | 2014-11-11 | |
| US201462078309P | 2014-11-11 | 2014-11-11 | |
| US62/078,309 | 2014-11-11 | ||
| US62/078,313 | 2014-11-11 | ||
| PCT/US2015/013994 WO2015117040A1 (en) | 2014-01-31 | 2015-01-30 | Improved methods for processing dna substrates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2015210705A1 AU2015210705A1 (en) | 2016-08-11 |
| AU2015210705B2 true AU2015210705B2 (en) | 2020-11-05 |
Family
ID=53757794
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2015210705A Active AU2015210705B2 (en) | 2014-01-31 | 2015-01-30 | Improved methods for processing DNA substrates |
Country Status (11)
| Country | Link |
|---|---|
| US (6) | US10316357B2 (enExample) |
| EP (5) | EP3604544B1 (enExample) |
| JP (2) | JP6723929B2 (enExample) |
| KR (2) | KR102482592B1 (enExample) |
| CN (2) | CN109971826B (enExample) |
| AU (1) | AU2015210705B2 (enExample) |
| CA (2) | CA2938213C (enExample) |
| DK (1) | DK3604544T3 (enExample) |
| ES (1) | ES2908644T3 (enExample) |
| HU (1) | HUE058149T2 (enExample) |
| WO (1) | WO2015117040A1 (enExample) |
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| US11859246B2 (en) | 2013-12-11 | 2024-01-02 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
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| US11286519B2 (en) | 2013-12-11 | 2022-03-29 | Accuragen Holdings Limited | Methods and compositions for enrichment of amplification products |
| KR102482592B1 (ko) | 2014-01-31 | 2022-12-30 | 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. | Dna 기질을 처리하는 개선 방법 |
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| CN106811460B (zh) * | 2015-11-30 | 2020-11-27 | 浙江安诺优达生物科技有限公司 | 用于低频突变检测的二代测序文库的构建方法及试剂盒 |
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| CN109563543B (zh) | 2016-07-15 | 2022-08-30 | 加利福尼亚大学董事会 | 产生核酸库的方法 |
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| KR102850460B1 (ko) | 2016-08-25 | 2025-08-27 | 레졸루션 바이오사이언스, 인크. | Dna 샘플 중 게놈 카피 변화의 검출을 위한 방법 |
| WO2018045109A1 (en) * | 2016-08-30 | 2018-03-08 | Metabiotech Corporation | Methods and compositions for phased sequencing |
| CN117512078A (zh) * | 2016-09-22 | 2024-02-06 | 西格马-奥尔德里奇有限责任公司 | 单引物至双引物扩增子转换 |
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