JP2015510766A5 - - Google Patents
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- JP2015510766A5 JP2015510766A5 JP2015500582A JP2015500582A JP2015510766A5 JP 2015510766 A5 JP2015510766 A5 JP 2015510766A5 JP 2015500582 A JP2015500582 A JP 2015500582A JP 2015500582 A JP2015500582 A JP 2015500582A JP 2015510766 A5 JP2015510766 A5 JP 2015510766A5
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- Prior art keywords
- attenuator
- molecule
- sequence
- polynucleotide
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108091033319 polynucleotide Proteins 0.000 claims description 119
- 102000040430 polynucleotide Human genes 0.000 claims description 119
- 239000002157 polynucleotide Substances 0.000 claims description 119
- 239000000758 substrate Substances 0.000 claims description 106
- 239000000203 mixture Substances 0.000 claims description 74
- 238000000034 method Methods 0.000 claims description 72
- 239000002773 nucleotide Substances 0.000 claims description 35
- 125000003729 nucleotide group Chemical group 0.000 claims description 35
- 230000000295 complement effect Effects 0.000 claims description 33
- 239000012082 adaptor molecule Substances 0.000 claims description 23
- 108020004414 DNA Proteins 0.000 claims description 22
- 229910019142 PO4 Inorganic materials 0.000 claims description 19
- 239000010452 phosphate Substances 0.000 claims description 19
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 19
- 229920002477 rna polymer Polymers 0.000 claims description 17
- 108090000364 Ligases Proteins 0.000 claims description 12
- 102000003960 Ligases Human genes 0.000 claims description 12
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical group O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 12
- 229920001519 homopolymer Polymers 0.000 claims description 12
- 108091028664 Ribonucleotide Proteins 0.000 claims description 10
- 238000011534 incubation Methods 0.000 claims description 10
- 239000002336 ribonucleotide Substances 0.000 claims description 10
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 10
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 9
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 claims description 9
- 102000039446 nucleic acids Human genes 0.000 claims description 8
- 108020004707 nucleic acids Proteins 0.000 claims description 8
- 150000007523 nucleic acids Chemical class 0.000 claims description 8
- 108010083644 Ribonucleases Proteins 0.000 claims description 6
- 102000006382 Ribonucleases Human genes 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- OLXZPDWKRNYJJZ-UHFFFAOYSA-N 5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-ol Chemical compound C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(CO)O1 OLXZPDWKRNYJJZ-UHFFFAOYSA-N 0.000 claims description 4
- 108010061982 DNA Ligases Proteins 0.000 claims description 4
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 claims description 4
- 101710163270 Nuclease Proteins 0.000 claims description 4
- 101710086015 RNA ligase Proteins 0.000 claims description 4
- DJJCXFVJDGTHFX-UHFFFAOYSA-N Uridinemonophosphate Natural products OC1C(O)C(COP(O)(O)=O)OC1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-UHFFFAOYSA-N 0.000 claims description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 4
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 claims description 4
- IERHLVCPSMICTF-XVFCMESISA-N cytidine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O1 IERHLVCPSMICTF-XVFCMESISA-N 0.000 claims description 4
- IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 125000006239 protecting group Chemical group 0.000 claims description 4
- DJJCXFVJDGTHFX-XVFCMESISA-N uridine 5'-monophosphate Chemical compound O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 claims description 4
- 102000053602 DNA Human genes 0.000 claims description 3
- 108090000119 Nucleotidyltransferases Proteins 0.000 claims description 3
- 102000003832 Nucleotidyltransferases Human genes 0.000 claims description 3
- 101710124239 Poly(A) polymerase Proteins 0.000 claims description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 claims description 3
- 239000005547 deoxyribonucleotide Substances 0.000 claims description 3
- 125000002637 deoxyribonucleotide group Chemical group 0.000 claims description 3
- -1 schizophyllan polysaccharides Chemical class 0.000 claims description 3
- NCMVOABPESMRCP-SHYZEUOFSA-N 2'-deoxycytosine 5'-monophosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 NCMVOABPESMRCP-SHYZEUOFSA-N 0.000 claims description 2
- YKBGVTZYEHREMT-KVQBGUIXSA-N 2'-deoxyguanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](CO)O1 YKBGVTZYEHREMT-KVQBGUIXSA-N 0.000 claims description 2
- YKBGVTZYEHREMT-UHFFFAOYSA-N 2'-deoxyguanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1CC(O)C(CO)O1 YKBGVTZYEHREMT-UHFFFAOYSA-N 0.000 claims description 2
- LTFMZDNNPPEQNG-KVQBGUIXSA-N 2'-deoxyguanosine 5'-monophosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 LTFMZDNNPPEQNG-KVQBGUIXSA-N 0.000 claims description 2
- IQFYYKKMVGJFEH-BIIVOSGPSA-N 2'-deoxythymidine Natural products O=C1NC(=O)C(C)=CN1[C@@H]1O[C@@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-BIIVOSGPSA-N 0.000 claims description 2
- MXHRCPNRJAMMIM-SHYZEUOFSA-N 2'-deoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-SHYZEUOFSA-N 0.000 claims description 2
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 claims description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 2
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 claims description 2
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 claims description 2
- 102000012410 DNA Ligases Human genes 0.000 claims description 2
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims description 2
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 2
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 2
- 102100031780 Endonuclease Human genes 0.000 claims description 2
- 108010042407 Endonucleases Proteins 0.000 claims description 2
- 102100034343 Integrase Human genes 0.000 claims description 2
- 101710203526 Integrase Proteins 0.000 claims description 2
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 claims description 2
- 102000005891 Pancreatic ribonuclease Human genes 0.000 claims description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 claims description 2
- 108010046983 Ribonuclease T1 Proteins 0.000 claims description 2
- 229920002305 Schizophyllan Polymers 0.000 claims description 2
- 229960005305 adenosine Drugs 0.000 claims description 2
- 125000003277 amino group Chemical group 0.000 claims description 2
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 claims description 2
- KHWCHTKSEGGWEX-UHFFFAOYSA-N deoxyadenylic acid Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(O)=O)O1 KHWCHTKSEGGWEX-UHFFFAOYSA-N 0.000 claims description 2
- LTFMZDNNPPEQNG-UHFFFAOYSA-N deoxyguanylic acid Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1CC(O)C(COP(O)(O)=O)O1 LTFMZDNNPPEQNG-UHFFFAOYSA-N 0.000 claims description 2
- VGONTNSXDCQUGY-UHFFFAOYSA-N desoxyinosine Natural products C1C(O)C(CO)OC1N1C(NC=NC2=O)=C2N=C1 VGONTNSXDCQUGY-UHFFFAOYSA-N 0.000 claims description 2
- MXHRCPNRJAMMIM-UHFFFAOYSA-N desoxyuridine Natural products C1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 MXHRCPNRJAMMIM-UHFFFAOYSA-N 0.000 claims description 2
- 239000005546 dideoxynucleotide Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 229940029575 guanosine Drugs 0.000 claims description 2
- 229920000140 heteropolymer Polymers 0.000 claims description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 108090000731 ribonuclease HII Proteins 0.000 claims description 2
- 125000006850 spacer group Chemical group 0.000 claims description 2
- 229940104230 thymidine Drugs 0.000 claims description 2
- 230000002238 attenuated effect Effects 0.000 claims 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
- 125000004122 cyclic group Chemical group 0.000 claims 1
- 238000000137 annealing Methods 0.000 description 9
- 239000011324 bead Substances 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 108010090804 Streptavidin Proteins 0.000 description 6
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 5
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 5
- 108020001738 DNA Glycosylase Proteins 0.000 description 3
- 102000028381 DNA glycosylase Human genes 0.000 description 3
- 108091034057 RNA (poly(A)) Proteins 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000003100 immobilizing effect Effects 0.000 description 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 230000000368 destabilizing effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000006287 biotinylation Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261610296P | 2012-03-13 | 2012-03-13 | |
| US61/610,296 | 2012-03-13 | ||
| US201261613784P | 2012-03-21 | 2012-03-21 | |
| US61/613,784 | 2012-03-21 | ||
| PCT/US2013/031104 WO2013138536A1 (en) | 2012-03-13 | 2013-03-13 | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018103405A Division JP2018130124A (ja) | 2012-03-13 | 2018-05-30 | 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015510766A JP2015510766A (ja) | 2015-04-13 |
| JP2015510766A5 true JP2015510766A5 (enExample) | 2016-05-12 |
| JP6509723B2 JP6509723B2 (ja) | 2019-05-08 |
Family
ID=49161798
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015500582A Active JP6509723B2 (ja) | 2012-03-13 | 2013-03-13 | 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物 |
| JP2018103405A Pending JP2018130124A (ja) | 2012-03-13 | 2018-05-30 | 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018103405A Pending JP2018130124A (ja) | 2012-03-13 | 2018-05-30 | 核酸ポリメラーゼによる基質ポリヌクレオチドの大きさ制御されたホモポリマーテーリングのための方法および組成物 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US9896709B2 (enExample) |
| EP (1) | EP2825672B1 (enExample) |
| JP (2) | JP6509723B2 (enExample) |
| CN (2) | CN108300765B (enExample) |
| AU (1) | AU2013232131B2 (enExample) |
| CA (1) | CA2866625C (enExample) |
| LT (1) | LT2825672T (enExample) |
| SG (1) | SG11201405669XA (enExample) |
| WO (1) | WO2013138536A1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
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| US9371598B2 (en) | 2010-04-05 | 2016-06-21 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| EP3150750B1 (en) | 2011-04-08 | 2018-12-26 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| CN108300765B (zh) * | 2012-03-13 | 2022-01-11 | 斯威夫特生物科学公司 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
| EP2895605A4 (en) * | 2012-09-13 | 2016-04-13 | Clontech Lab Inc | PROCESS FOR PREPARING A TARGET NUCLEIC ACID IN A SAMPLE AND KITS FOR CARRYING OUT THIS METHOD |
| EP3511423B2 (en) | 2012-10-17 | 2024-05-29 | Spatial Transcriptomics AB | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| US11231419B2 (en) | 2013-03-15 | 2022-01-25 | Prognosys Biosciences, Inc. | Methods for detecting peptide/MHC/TCR binding |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| JP6556728B2 (ja) * | 2013-09-13 | 2019-08-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | ポリＡ−キャリヤーにより誘導される高分子量ＰＣＲ生成物の生成を避けるためのオリゴ−ｄＴ分子の適用 |
| CA2921067A1 (en) * | 2013-09-30 | 2015-04-02 | Qiagen Gmbh | Dna-adapter-molecules for the preparation of dna-libraries and method for producing them and use |
| WO2015070037A2 (en) | 2013-11-08 | 2015-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| EP4273264A3 (en) | 2014-01-31 | 2024-01-17 | Integrated DNA Technologies, Inc. | Improved methods for processing dna substrates |
| WO2015134552A1 (en) * | 2014-03-03 | 2015-09-11 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| LT3207134T (lt) | 2014-10-17 | 2019-09-10 | Illumina Cambridge Limited | Sukibimą išsauganti transpozicija |
| JP6808617B2 (ja) | 2014-10-17 | 2021-01-06 | イルミナ ケンブリッジ リミテッド | 連続性を維持した転位 |
| SG11201707515SA (en) | 2015-04-10 | 2017-10-30 | Spatial Transcriptomics Ab | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| CA2994601C (en) * | 2015-08-06 | 2020-08-25 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
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| EP3485038B1 (en) * | 2016-07-12 | 2020-12-23 | H. Hoffnabb-La Roche Ag | Primer extension target enrichment |
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| WO2019030149A1 (en) | 2017-08-07 | 2019-02-14 | Dna Script | VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF |
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| WO2019191900A1 (en) * | 2018-04-03 | 2019-10-10 | Burning Rock Biotech | Compositions and methods for preparing nucleic acid libraries |
| EP3826762A1 (en) | 2018-07-23 | 2021-06-02 | DNA Script | Massively parallel enzymatic synthesis of nucleic acid strands |
| US10696994B2 (en) * | 2018-09-28 | 2020-06-30 | Bioo Scientific Corporation | Size selection of RNA using poly(A) polymerase |
| CN111989406B (zh) * | 2018-10-11 | 2023-02-21 | 北京优乐复生科技有限责任公司 | 一种测序文库的构建方法 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US10961562B2 (en) | 2016-09-06 | 2021-03-30 | Swift Biosciences, Inc. | Normalization of NGS library concentration |
| US12371731B2 (en) | 2016-09-06 | 2025-07-29 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
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