CN108300765B - 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 - Google Patents
用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 Download PDFInfo
- Publication number
- CN108300765B CN108300765B CN201810004450.6A CN201810004450A CN108300765B CN 108300765 B CN108300765 B CN 108300765B CN 201810004450 A CN201810004450 A CN 201810004450A CN 108300765 B CN108300765 B CN 108300765B
- Authority
- CN
- China
- Prior art keywords
- attenuator
- polynucleotide
- sequence
- dna
- poly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/131—Terminal transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/173—Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/204—Modifications characterised by specific length of the oligonucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261610296P | 2012-03-13 | 2012-03-13 | |
| US61/610,296 | 2012-03-13 | ||
| US201261613784P | 2012-03-21 | 2012-03-21 | |
| US61/613,784 | 2012-03-21 | ||
| CN201380014044.3A CN104395480B (zh) | 2012-03-13 | 2013-03-13 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380014044.3A Division CN104395480B (zh) | 2012-03-13 | 2013-03-13 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108300765A CN108300765A (zh) | 2018-07-20 |
| CN108300765B true CN108300765B (zh) | 2022-01-11 |
Family
ID=49161798
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380014044.3A Active CN104395480B (zh) | 2012-03-13 | 2013-03-13 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
| CN201810004450.6A Active CN108300765B (zh) | 2012-03-13 | 2013-03-13 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380014044.3A Active CN104395480B (zh) | 2012-03-13 | 2013-03-13 | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US9896709B2 (enExample) |
| EP (1) | EP2825672B1 (enExample) |
| JP (2) | JP6509723B2 (enExample) |
| CN (2) | CN104395480B (enExample) |
| AU (1) | AU2013232131B2 (enExample) |
| CA (1) | CA2866625C (enExample) |
| LT (1) | LT2825672T (enExample) |
| SG (1) | SG11201405669XA (enExample) |
| WO (1) | WO2013138536A1 (enExample) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US20120258871A1 (en) | 2011-04-08 | 2012-10-11 | Prognosys Biosciences, Inc. | Peptide constructs and assay systems |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| SG11201405669XA (en) * | 2012-03-13 | 2014-10-30 | Swift Biosciences Inc | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
| WO2014043133A1 (en) * | 2012-09-13 | 2014-03-20 | Clontech Laboratories, Inc. | Methods of depleting a target nucleic acid in a sample and kits for practicing the same |
| USRE50065E1 (en) | 2012-10-17 | 2024-07-30 | 10X Genomics Sweden Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| EP3736573A1 (en) | 2013-03-15 | 2020-11-11 | Prognosys Biosciences, Inc. | Methods for detecting peptide/mhc/tcr binding |
| CN105849275B (zh) | 2013-06-25 | 2020-03-17 | 普罗格诺西斯生物科学公司 | 检测样品中生物靶标的空间分布的方法和系统 |
| EP3044326B1 (en) * | 2013-09-13 | 2017-12-06 | F. Hoffmann-La Roche AG | Application of oligo-dt molecules to avoid generation of high molecular pcr products induced by polya-carrier |
| EP3052645B1 (en) * | 2013-09-30 | 2019-01-09 | Qiagen GmbH | Dna-adapter-molecules for the preparation of dna-libraries and method for producing them and use |
| US10288608B2 (en) | 2013-11-08 | 2019-05-14 | Prognosys Biosciences, Inc. | Polynucleotide conjugates and methods for analyte detection |
| DK3604544T3 (en) | 2014-01-31 | 2022-02-14 | Swift Biosciences Inc | Improved methods for processing dna substrates |
| EP3114231B1 (en) * | 2014-03-03 | 2019-01-02 | Swift Biosciences, Inc. | Enhanced adaptor ligation |
| FR3020071B1 (fr) | 2014-04-17 | 2017-12-22 | Dna Script | Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede |
| FR3025201B1 (fr) | 2014-09-02 | 2018-10-12 | Dna Script | Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques |
| PT3207134T (pt) | 2014-10-17 | 2019-09-17 | Illumina Cambridge Ltd | Transposição que preserva a contiguidade |
| CA2964799A1 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| CA2994601C (en) * | 2015-08-06 | 2020-08-25 | F. Hoffmann-La Roche Ag | Target enrichment by single probe primer extension |
| AU2016313095A1 (en) * | 2015-08-24 | 2017-11-23 | Qiagen Gmbh | Method for generating a RNA-sequencing library |
| EP3485038B1 (en) * | 2016-07-12 | 2020-12-23 | H. Hoffnabb-La Roche Ag | Primer extension target enrichment |
| AU2017324946C1 (en) | 2016-09-06 | 2022-06-09 | Integrated Dna Technologies, Inc. | Normalization of NGS library concentration |
| CA3059370C (en) | 2017-04-12 | 2022-05-10 | Karius, Inc. | Methods for concurrent analysis of dna and rna in mixed samples |
| EP3665274B1 (en) | 2017-08-07 | 2025-10-15 | DNA Script | Variants of family a dna polymerase and uses thereof |
| WO2019055819A1 (en) * | 2017-09-14 | 2019-03-21 | Grail, Inc. | METHODS FOR PREPARING A SEQUENCING LIBRARY FROM SINGLE STRANDED DNA |
| WO2019090482A1 (zh) * | 2017-11-07 | 2019-05-16 | 北京优乐复生科技有限责任公司 | 一种第二代高通量测序文库构建方法 |
| EP3737749A1 (en) | 2018-01-08 | 2020-11-18 | DNA Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| US10752887B2 (en) | 2018-01-08 | 2020-08-25 | Dna Script | Variants of terminal deoxynucleotidyl transferase and uses thereof |
| WO2019191900A1 (en) | 2018-04-03 | 2019-10-10 | Burning Rock Biotech | Compositions and methods for preparing nucleic acid libraries |
| CN113056327A (zh) | 2018-07-23 | 2021-06-29 | Dna斯克瑞普特公司 | 核酸链的大规模平行酶促合成 |
| US10696994B2 (en) * | 2018-09-28 | 2020-06-30 | Bioo Scientific Corporation | Size selection of RNA using poly(A) polymerase |
| EP3865584A4 (en) * | 2018-10-11 | 2021-12-08 | Beijing Euler Technology Limited Company | PROCEDURE FOR CREATING A SEQUENCING LIBRARY |
| WO2020123318A1 (en) | 2018-12-10 | 2020-06-18 | 10X Genomics, Inc. | Resolving spatial arrays using deconvolution |
| AU2019398307A1 (en) | 2018-12-13 | 2021-06-10 | Dna Script | Direct oligonucleotide synthesis on cells and biomolecules |
| US11649485B2 (en) | 2019-01-06 | 2023-05-16 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| CN113423840B (zh) | 2019-02-12 | 2024-03-08 | Dna斯克瑞普特公司 | 多核苷酸无模板酶促合成中有效的产物裂解 |
| CN110055316A (zh) * | 2019-03-28 | 2019-07-26 | 天津大学 | 一种用于检测微小rna的由末端脱氧核苷酸转移酶介导的反转录pcr方法 |
| CN110628886B (zh) | 2019-09-05 | 2022-09-30 | 华侨大学 | 一种检测dna中的单链断裂的方法 |
| JP7740708B2 (ja) * | 2019-12-25 | 2025-09-17 | ナパジェン ファーマ,インコーポレテッド | ポリデオキシアデニル酸が連結した短鎖CpG含有オリゴデオキシヌクレオチド、該オリゴデオキシヌクレオチドを含有する複合体、及びその用途 |
| US11821035B1 (en) | 2020-01-29 | 2023-11-21 | 10X Genomics, Inc. | Compositions and methods of making gene expression libraries |
| US12076701B2 (en) | 2020-01-31 | 2024-09-03 | 10X Genomics, Inc. | Capturing oligonucleotides in spatial transcriptomics |
| US12110541B2 (en) | 2020-02-03 | 2024-10-08 | 10X Genomics, Inc. | Methods for preparing high-resolution spatial arrays |
| US12270026B1 (en) | 2020-03-05 | 2025-04-08 | Ansa Biotechnologies, Inc. | Directed evolution method for template-independent polymerases |
| US12359193B2 (en) | 2022-03-04 | 2025-07-15 | Element Biosciences, Inc. | Single-stranded splint strands and methods of use |
| US12371743B2 (en) | 2022-03-04 | 2025-07-29 | Element Biosciences, Inc. | Double-stranded splint adaptors and methods of use |
| KR102753901B1 (ko) * | 2021-08-17 | 2025-01-16 | 에스케이바이오사이언스 주식회사 | 폴리 a 테일을 안정적으로 유지하는 방법 |
| US11680293B1 (en) | 2022-04-21 | 2023-06-20 | Paragon Genomics, Inc. | Methods and compositions for amplifying DNA and generating DNA sequencing results from target-enriched DNA molecules |
| US12091715B2 (en) | 2022-04-21 | 2024-09-17 | Paragon Genomics, Inc. | Methods and compositions for reducing base errors of massive parallel sequencing using triseq sequencing |
| CA3267226A1 (en) | 2022-09-12 | 2024-03-21 | Element Biosciences, Inc. | DOUBLE STRAND TO STRAND SPLINT ADAPTERS, UNIVERSAL LONG SPLINTS AND METHODS OF USE |
| GB202308392D0 (en) | 2023-06-06 | 2023-07-19 | Fu Guoliang | Methods, compositions, and kits for preparing library and targeted sequencing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5334515A (en) * | 1991-04-10 | 1994-08-02 | Life Technologies, Inc. | Method for altering a nucleotide sequence |
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
Family Cites Families (116)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3687808A (en) | 1969-08-14 | 1972-08-29 | Univ Leland Stanford Junior | Synthetic polynucleotides |
| US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5023243A (en) | 1981-10-23 | 1991-06-11 | Molecular Biosystems, Inc. | Oligonucleotide therapeutic agent and method of making same |
| US4476301A (en) | 1982-04-29 | 1984-10-09 | Centre National De La Recherche Scientifique | Oligonucleotides, a process for preparing the same and their application as mediators of the action of interferon |
| US5118800A (en) | 1983-12-20 | 1992-06-02 | California Institute Of Technology | Oligonucleotides possessing a primary amino group in the terminal nucleotide |
| US5550111A (en) | 1984-07-11 | 1996-08-27 | Temple University-Of The Commonwealth System Of Higher Education | Dual action 2',5'-oligoadenylate antiviral derivatives and uses thereof |
| FR2567892B1 (fr) | 1984-07-19 | 1989-02-17 | Centre Nat Rech Scient | Nouveaux oligonucleotides, leur procede de preparation et leurs applications comme mediateurs dans le developpement des effets des interferons |
| US5367066A (en) | 1984-10-16 | 1994-11-22 | Chiron Corporation | Oligonucleotides with selectably cleavable and/or abasic sites |
| FR2575751B1 (fr) | 1985-01-08 | 1987-04-03 | Pasteur Institut | Nouveaux nucleosides de derives de l'adenosine, leur preparation et leurs applications biologiques |
| US5405938A (en) | 1989-12-20 | 1995-04-11 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
| US5185444A (en) | 1985-03-15 | 1993-02-09 | Anti-Gene Deveopment Group | Uncharged morpolino-based polymers having phosphorous containing chiral intersubunit linkages |
| US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
| US5166315A (en) | 1989-12-20 | 1992-11-24 | Anti-Gene Development Group | Sequence-specific binding polymers for duplex nucleic acids |
| US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
| US5264423A (en) | 1987-03-25 | 1993-11-23 | The United States Of America As Represented By The Department Of Health And Human Services | Inhibitors for replication of retroviruses and for the expression of oncogene products |
| US5276019A (en) | 1987-03-25 | 1994-01-04 | The United States Of America As Represented By The Department Of Health And Human Services | Inhibitors for replication of retroviruses and for the expression of oncogene products |
| CA1340032C (en) | 1987-06-24 | 1998-09-08 | Jim Haralambidis | Lucleoside derivatives |
| US5188897A (en) | 1987-10-22 | 1993-02-23 | Temple University Of The Commonwealth System Of Higher Education | Encapsulated 2',5'-phosphorothioate oligoadenylates |
| US4924624A (en) | 1987-10-22 | 1990-05-15 | Temple University-Of The Commonwealth System Of Higher Education | 2,',5'-phosphorothioate oligoadenylates and plant antiviral uses thereof |
| WO1989009221A1 (en) | 1988-03-25 | 1989-10-05 | University Of Virginia Alumni Patents Foundation | Oligonucleotide n-alkylphosphoramidates |
| US5278302A (en) | 1988-05-26 | 1994-01-11 | University Patents, Inc. | Polynucleotide phosphorodithioates |
| US5216141A (en) | 1988-06-06 | 1993-06-01 | Benner Steven A | Oligonucleotide analogs containing sulfur linkages |
| US5175273A (en) | 1988-07-01 | 1992-12-29 | Genentech, Inc. | Nucleic acid intercalating agents |
| US5194599A (en) | 1988-09-23 | 1993-03-16 | Gilead Sciences, Inc. | Hydrogen phosphonodithioate compositions |
| US5134066A (en) | 1989-08-29 | 1992-07-28 | Monsanto Company | Improved probes using nucleosides containing 3-dezauracil analogs |
| US5591722A (en) | 1989-09-15 | 1997-01-07 | Southern Research Institute | 2'-deoxy-4'-thioribonucleosides and their antiviral activity |
| US5721218A (en) | 1989-10-23 | 1998-02-24 | Gilead Sciences, Inc. | Oligonucleotides with inverted polarity |
| US5399676A (en) | 1989-10-23 | 1995-03-21 | Gilead Sciences | Oligonucleotides with inverted polarity |
| ATE190981T1 (de) | 1989-10-24 | 2000-04-15 | Isis Pharmaceuticals Inc | 2'-modifizierte nukleotide |
| US5264564A (en) | 1989-10-24 | 1993-11-23 | Gilead Sciences | Oligonucleotide analogs with novel linkages |
| US5264562A (en) | 1989-10-24 | 1993-11-23 | Gilead Sciences, Inc. | Oligonucleotide analogs with novel linkages |
| US5177198A (en) | 1989-11-30 | 1993-01-05 | University Of N.C. At Chapel Hill | Process for preparing oligoribonucleoside and oligodeoxyribonucleoside boranophosphates |
| US5130302A (en) | 1989-12-20 | 1992-07-14 | Boron Bilogicals, Inc. | Boronated nucleoside, nucleotide and oligonucleotide compounds, compositions and methods for using same |
| US5681941A (en) | 1990-01-11 | 1997-10-28 | Isis Pharmaceuticals, Inc. | Substituted purines and oligonucleotide cross-linking |
| US5670633A (en) | 1990-01-11 | 1997-09-23 | Isis Pharmaceuticals, Inc. | Sugar modified oligonucleotides that detect and modulate gene expression |
| US5587470A (en) | 1990-01-11 | 1996-12-24 | Isis Pharmaceuticals, Inc. | 3-deazapurines |
| US5587361A (en) | 1991-10-15 | 1996-12-24 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
| US5646265A (en) | 1990-01-11 | 1997-07-08 | Isis Pharmceuticals, Inc. | Process for the preparation of 2'-O-alkyl purine phosphoramidites |
| US5459255A (en) | 1990-01-11 | 1995-10-17 | Isis Pharmaceuticals, Inc. | N-2 substituted purines |
| US5321131A (en) | 1990-03-08 | 1994-06-14 | Hybridon, Inc. | Site-specific functionalization of oligodeoxynucleotides for non-radioactive labelling |
| US5470967A (en) | 1990-04-10 | 1995-11-28 | The Dupont Merck Pharmaceutical Company | Oligonucleotide analogs with sulfamate linkages |
| GB9009980D0 (en) | 1990-05-03 | 1990-06-27 | Amersham Int Plc | Phosphoramidite derivatives,their preparation and the use thereof in the incorporation of reporter groups on synthetic oligonucleotides |
| DE69032425T2 (de) | 1990-05-11 | 1998-11-26 | Microprobe Corp., Bothell, Wash. | Teststreifen zum Eintauchen für Nukleinsäure-Hybridisierungsassays und Verfahren zur kovalenten Immobilisierung von Oligonucleotiden |
| US5608046A (en) | 1990-07-27 | 1997-03-04 | Isis Pharmaceuticals, Inc. | Conjugated 4'-desmethyl nucleoside analog compounds |
| US5541307A (en) | 1990-07-27 | 1996-07-30 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogs and solid phase synthesis thereof |
| US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
| US5614617A (en) | 1990-07-27 | 1997-03-25 | Isis Pharmaceuticals, Inc. | Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression |
| US5677437A (en) | 1990-07-27 | 1997-10-14 | Isis Pharmaceuticals, Inc. | Heteroatomic oligonucleoside linkages |
| US5618704A (en) | 1990-07-27 | 1997-04-08 | Isis Pharmacueticals, Inc. | Backbone-modified oligonucleotide analogs and preparation thereof through radical coupling |
| US5610289A (en) | 1990-07-27 | 1997-03-11 | Isis Pharmaceuticals, Inc. | Backbone modified oligonucleotide analogues |
| US5623070A (en) | 1990-07-27 | 1997-04-22 | Isis Pharmaceuticals, Inc. | Heteroatomic oligonucleoside linkages |
| US5489677A (en) | 1990-07-27 | 1996-02-06 | Isis Pharmaceuticals, Inc. | Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms |
| PT98562B (pt) | 1990-08-03 | 1999-01-29 | Sanofi Sa | Processo para a preparacao de composicoes que compreendem sequencias de nucleo-sidos com cerca de 6 a cerca de 200 bases resistentes a nucleases |
| US5177196A (en) | 1990-08-16 | 1993-01-05 | Microprobe Corporation | Oligo (α-arabinofuranosyl nucleotides) and α-arabinofuranosyl precursors thereof |
| US5214134A (en) | 1990-09-12 | 1993-05-25 | Sterling Winthrop Inc. | Process of linking nucleosides with a siloxane bridge |
| US5561225A (en) | 1990-09-19 | 1996-10-01 | Southern Research Institute | Polynucleotide analogs containing sulfonate and sulfonamide internucleoside linkages |
| AU662298B2 (en) | 1990-09-20 | 1995-08-31 | Gilead Sciences, Inc. | Modified internucleoside linkages |
| US5432272A (en) | 1990-10-09 | 1995-07-11 | Benner; Steven A. | Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases |
| US5672697A (en) | 1991-02-08 | 1997-09-30 | Gilead Sciences, Inc. | Nucleoside 5'-methylene phosphonates |
| US5719262A (en) | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
| US5714331A (en) | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
| US7223833B1 (en) | 1991-05-24 | 2007-05-29 | Isis Pharmaceuticals, Inc. | Peptide nucleic acid conjugates |
| US5539082A (en) | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
| US5571799A (en) | 1991-08-12 | 1996-11-05 | Basco, Ltd. | (2'-5') oligoadenylate analogues useful as inhibitors of host-v5.-graft response |
| ES2103918T3 (es) | 1991-10-17 | 1997-10-01 | Ciba Geigy Ag | Nucleosidos biciclicos, oligonucleotidos, procedimiento para su obtencion y productos intermedios. |
| US5594121A (en) | 1991-11-07 | 1997-01-14 | Gilead Sciences, Inc. | Enhanced triple-helix and double-helix formation with oligomers containing modified purines |
| US5484908A (en) | 1991-11-26 | 1996-01-16 | Gilead Sciences, Inc. | Oligonucleotides containing 5-propynyl pyrimidines |
| TW393513B (en) | 1991-11-26 | 2000-06-11 | Isis Pharmaceuticals Inc | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
| JP3739785B2 (ja) | 1991-11-26 | 2006-01-25 | アイシス ファーマシューティカルズ,インコーポレイティド | 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形 |
| US5792608A (en) | 1991-12-12 | 1998-08-11 | Gilead Sciences, Inc. | Nuclease stable and binding competent oligomers and methods for their use |
| US5359044A (en) | 1991-12-13 | 1994-10-25 | Isis Pharmaceuticals | Cyclobutyl oligonucleotide surrogates |
| FR2687679B1 (fr) | 1992-02-05 | 1994-10-28 | Centre Nat Rech Scient | Oligothionucleotides. |
| US5633360A (en) | 1992-04-14 | 1997-05-27 | Gilead Sciences, Inc. | Oligonucleotide analogs capable of passive cell membrane permeation |
| US5434257A (en) | 1992-06-01 | 1995-07-18 | Gilead Sciences, Inc. | Binding compentent oligomers containing unsaturated 3',5' and 2',5' linkages |
| EP0577558A2 (de) | 1992-07-01 | 1994-01-05 | Ciba-Geigy Ag | Carbocyclische Nukleoside mit bicyclischen Ringen, Oligonukleotide daraus, Verfahren zu deren Herstellung, deren Verwendung und Zwischenproduckte |
| US5476925A (en) | 1993-02-01 | 1995-12-19 | Northwestern University | Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups |
| GB9304618D0 (en) | 1993-03-06 | 1993-04-21 | Ciba Geigy Ag | Chemical compounds |
| DE69404289T2 (de) | 1993-03-30 | 1998-02-19 | Sanofi Sa | Acyclische nucleosid analoge und sie enthaltende oligonucleotidsequenzen |
| ATE160572T1 (de) | 1993-03-31 | 1997-12-15 | Sanofi Sa | Oligonucleotide mit amidverkettungen die phosphoesterverkettungen einsetzen |
| DE4311944A1 (de) | 1993-04-10 | 1994-10-13 | Degussa | Umhüllte Natriumpercarbonatpartikel, Verfahren zu deren Herstellung und sie enthaltende Wasch-, Reinigungs- und Bleichmittelzusammensetzungen |
| US5502177A (en) | 1993-09-17 | 1996-03-26 | Gilead Sciences, Inc. | Pyrimidine derivatives for labeled binding partners |
| US5457187A (en) | 1993-12-08 | 1995-10-10 | Board Of Regents University Of Nebraska | Oligonucleotides containing 5-fluorouracil |
| US5446137B1 (en) | 1993-12-09 | 1998-10-06 | Behringwerke Ag | Oligonucleotides containing 4'-substituted nucleotides |
| US5519134A (en) | 1994-01-11 | 1996-05-21 | Isis Pharmaceuticals, Inc. | Pyrrolidine-containing monomers and oligomers |
| US5596091A (en) | 1994-03-18 | 1997-01-21 | The Regents Of The University Of California | Antisense oligonucleotides comprising 5-aminoalkyl pyrimidine nucleotides |
| US5627053A (en) | 1994-03-29 | 1997-05-06 | Ribozyme Pharmaceuticals, Inc. | 2'deoxy-2'-alkylnucleotide containing nucleic acid |
| US5625050A (en) | 1994-03-31 | 1997-04-29 | Amgen Inc. | Modified oligonucleotides and intermediates useful in nucleic acid therapeutics |
| US5646269A (en) | 1994-04-28 | 1997-07-08 | Gilead Sciences, Inc. | Method for oligonucleotide analog synthesis |
| US5525711A (en) | 1994-05-18 | 1996-06-11 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Pteridine nucleotide analogs as fluorescent DNA probes |
| US5597909A (en) | 1994-08-25 | 1997-01-28 | Chiron Corporation | Polynucleotide reagents containing modified deoxyribose moieties, and associated methods of synthesis and use |
| US5792747A (en) | 1995-01-24 | 1998-08-11 | The Administrators Of The Tulane Educational Fund | Highly potent agonists of growth hormone releasing hormone |
| US5912340A (en) | 1995-10-04 | 1999-06-15 | Epoch Pharmaceuticals, Inc. | Selective binding complementary oligonucleotides |
| DE19601385A1 (de) * | 1996-01-16 | 1997-07-17 | Mueller Manfred W | Verfahren zur Amplifikation von Nukleinsäure |
| JP3756313B2 (ja) | 1997-03-07 | 2006-03-15 | 武 今西 | 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体 |
| WO1999014226A2 (en) | 1997-09-12 | 1999-03-25 | Exiqon A/S | Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues |
| DE19920611A1 (de) | 1999-05-05 | 2000-11-09 | Roche Diagnostics Gmbh | Verfahren zur 5'-Cap-abhängigen Anreicherung von cDNAs |
| EP1072679A3 (en) | 1999-07-20 | 2002-07-31 | Agilent Technologies, Inc. (a Delaware corporation) | Method of producing nucleic acid molecules with reduced secondary structure |
| US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
| US6242189B1 (en) | 1999-10-01 | 2001-06-05 | The Regents Of The University Of California | Selective isolation of bacterial mRNA |
| JP2005535283A (ja) * | 2001-11-13 | 2005-11-24 | ルビコン ゲノミクス インコーポレイテッド | ランダムフラグメント化により生成されたdna分子を用いたdna増幅および配列決定 |
| US20040219565A1 (en) | 2002-10-21 | 2004-11-04 | Sakari Kauppinen | Oligonucleotides useful for detecting and analyzing nucleic acids of interest |
| DE602004029560D1 (de) * | 2003-03-07 | 2010-11-25 | Rubicon Genomics Inc | Amplifikation und analyse von gesamtgenom- und gesamttranskriptom- bibliotheken, die durch ein dns-polymerisierungsverfahren produziert wurden |
| US20040209299A1 (en) | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| DE602004022282D1 (de) | 2003-05-09 | 2009-09-10 | Genisphere Inc | Verfahren zur amplifikation von nukleinsäuresequenzen mittels gestaffelter ligation |
| WO2005098044A1 (en) * | 2004-04-01 | 2005-10-20 | Genisphere Inc. | Methods for amplification of nucleic acid sequences using promoter templates |
| US7361465B2 (en) | 2004-09-07 | 2008-04-22 | Applera Corporation | Methods and compositions for tailing and amplifying RNA |
| CN101203618B (zh) | 2005-05-26 | 2013-03-13 | 人类遗传标记控股有限公司 | 使用含有非常规碱基的引物的等温链置换扩增 |
| US7550264B2 (en) * | 2005-06-10 | 2009-06-23 | Datascope Investment Corporation | Methods and kits for sense RNA synthesis |
| EP1907571B1 (en) * | 2005-06-15 | 2017-04-26 | Complete Genomics Inc. | Nucleic acid analysis by random mixtures of non-overlapping fragments |
| US20100190167A1 (en) * | 2007-02-14 | 2010-07-29 | Genisphere Inc. | Methods, Reagents and Kits for Detection of Nucleic Acid Molecules |
| US20110129827A1 (en) * | 2008-04-04 | 2011-06-02 | Helicos Biosciences Corporation | Methods for transcript analysis |
| EP2283132B1 (en) | 2008-05-02 | 2016-10-26 | Epicentre Technologies Corporation | Selective 5' ligation tagging of rna |
| EP2362910A4 (en) * | 2008-11-03 | 2012-05-09 | Alchemy Biosciences Pty Ltd | METHOD FOR DNA CLONING |
| WO2010120554A1 (en) * | 2009-04-01 | 2010-10-21 | The Regents Of The University Of California | Detecting and treating breast cancer resistance to egfr inhibitors |
| EP2280081A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of RNA |
| SG11201405669XA (en) * | 2012-03-13 | 2014-10-30 | Swift Biosciences Inc | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase |
-
2013
- 2013-03-13 SG SG11201405669XA patent/SG11201405669XA/en unknown
- 2013-03-13 EP EP13761457.4A patent/EP2825672B1/en active Active
- 2013-03-13 US US14/384,113 patent/US9896709B2/en active Active
- 2013-03-13 LT LTEP13761457.4T patent/LT2825672T/lt unknown
- 2013-03-13 CN CN201380014044.3A patent/CN104395480B/zh active Active
- 2013-03-13 AU AU2013232131A patent/AU2013232131B2/en active Active
- 2013-03-13 JP JP2015500582A patent/JP6509723B2/ja active Active
- 2013-03-13 CN CN201810004450.6A patent/CN108300765B/zh active Active
- 2013-03-13 CA CA2866625A patent/CA2866625C/en active Active
- 2013-03-13 WO PCT/US2013/031104 patent/WO2013138536A1/en not_active Ceased
-
2018
- 2018-02-17 US US15/898,460 patent/US10731194B2/en active Active
- 2018-05-30 JP JP2018103405A patent/JP2018130124A/ja active Pending
-
2020
- 2020-06-19 US US16/906,165 patent/US11118207B2/en active Active
-
2021
- 2021-08-10 US US17/398,967 patent/US12104194B2/en active Active
-
2024
- 2024-09-04 US US18/824,005 patent/US20250084448A1/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6114149A (en) * | 1988-07-26 | 2000-09-05 | Genelabs Technologies, Inc. | Amplification of mixed sequence nucleic acid fragments |
| US5334515A (en) * | 1991-04-10 | 1994-08-02 | Life Technologies, Inc. | Method for altering a nucleotide sequence |
Also Published As
| Publication number | Publication date |
|---|---|
| LT2825672T (lt) | 2019-06-10 |
| CN108300765A (zh) | 2018-07-20 |
| JP2018130124A (ja) | 2018-08-23 |
| CN104395480B (zh) | 2018-01-30 |
| US11118207B2 (en) | 2021-09-14 |
| US20220186273A1 (en) | 2022-06-16 |
| US20150087027A1 (en) | 2015-03-26 |
| US12104194B2 (en) | 2024-10-01 |
| CA2866625A1 (en) | 2013-09-19 |
| WO2013138536A1 (en) | 2013-09-19 |
| US20180223321A1 (en) | 2018-08-09 |
| EP2825672A1 (en) | 2015-01-21 |
| AU2013232131B2 (en) | 2018-09-20 |
| SG11201405669XA (en) | 2014-10-30 |
| JP6509723B2 (ja) | 2019-05-08 |
| AU2013232131A1 (en) | 2014-09-18 |
| US9896709B2 (en) | 2018-02-20 |
| US20200392551A1 (en) | 2020-12-17 |
| HK1206395A1 (en) | 2016-01-08 |
| CN104395480A (zh) | 2015-03-04 |
| US10731194B2 (en) | 2020-08-04 |
| EP2825672A4 (en) | 2015-11-18 |
| CA2866625C (en) | 2020-12-08 |
| JP2015510766A (ja) | 2015-04-13 |
| US20250084448A1 (en) | 2025-03-13 |
| EP2825672B1 (en) | 2019-02-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108300765B (zh) | 用于通过核酸聚合酶对衬底多核苷酸进行大小受控的同聚物加尾的方法和组合物 | |
| US9328378B2 (en) | Method of library preparation avoiding the formation of adaptor dimers | |
| US11041851B2 (en) | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction | |
| EP2585593B1 (en) | Methods for polynucleotide library production, immortalization and region of interest extraction | |
| JP7026248B2 (ja) | 二本鎖dnaを増幅するための方法およびキット | |
| CN104080958A (zh) | 用于定向核酸扩增和测序的组合物和方法 | |
| CN120796437A (zh) | 用于制备核酸文库的方法、组合物和试剂盒 | |
| EP2191011A1 (en) | Method for sequencing a polynucleotide template | |
| JP2013518598A (ja) | ランダム化配列を含むプライマー及び特異的プライマーを用いる核酸の等温増幅並びにその使用 | |
| CN111542532B (zh) | 酶法合成寡核苷酸的方法和体系 | |
| JP7191115B2 (ja) | エンドヌクレアーゼ媒介移動平衡(EM-SEq)による核酸の増幅のための方法 | |
| EP2504450B1 (en) | Devices to extend single stranded target molecules | |
| HK1206395B (en) | Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase | |
| WO2025003669A1 (en) | Polynucleotide synthesis method, kit and system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230309 Address after: Iowa, USA Patentee after: INTEGRATED DNA TECHNOLOGIES, Inc. Address before: Michigan, USA Patentee before: SWIFT BIOSCIENCES, Inc. |
|
| TR01 | Transfer of patent right |