PT3207134T - Transposição que preserva a contiguidade - Google Patents

Transposição que preserva a contiguidade

Info

Publication number
PT3207134T
PT3207134T PT15797490T PT15797490T PT3207134T PT 3207134 T PT3207134 T PT 3207134T PT 15797490 T PT15797490 T PT 15797490T PT 15797490 T PT15797490 T PT 15797490T PT 3207134 T PT3207134 T PT 3207134T
Authority
PT
Portugal
Prior art keywords
contiguity preserving
preserving transposition
transposition
contiguity
preserving
Prior art date
Application number
PT15797490T
Other languages
English (en)
Inventor
J Steemers Frank
L Gunderson Kevin
Zhang Fan
Richard Betley Jason
Anthony Gormley Niall
Meuleman Wouter
Weir Jacqueline
Ioannou Avgousta
Jenkins Gareth
Jackson Rosamond
Morrell Natalie
K Pokholok Dmitry
J Norberg Steven
He Molly
Kia Amirali
Goryshin Igor
Pantoja Rigo
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of PT3207134T publication Critical patent/PT3207134T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PT15797490T 2014-10-17 2015-10-16 Transposição que preserva a contiguidade PT3207134T (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462065544P 2014-10-17 2014-10-17
US201562157396P 2015-05-05 2015-05-05

Publications (1)

Publication Number Publication Date
PT3207134T true PT3207134T (pt) 2019-09-17

Family

ID=54601997

Family Applications (1)

Application Number Title Priority Date Filing Date
PT15797490T PT3207134T (pt) 2014-10-17 2015-10-16 Transposição que preserva a contiguidade

Country Status (6)

Country Link
EP (2) EP3636757A1 (pt)
CN (2) CN107969137B (pt)
DK (1) DK3207134T3 (pt)
ES (1) ES2745814T3 (pt)
LT (1) LT3207134T (pt)
PT (1) PT3207134T (pt)

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* Cited by examiner, † Cited by third party
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CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN112272710A (zh) * 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
WO2020069350A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
CN112739829A (zh) * 2018-09-27 2021-04-30 深圳华大生命科学研究院 测序文库的构建方法和得到的测序文库及测序方法
ES2960890T3 (es) * 2018-11-30 2024-03-07 Illumina Inc Análisis de múltiples analitos utilizando un solo ensayo
US20220154173A1 (en) * 2019-07-12 2022-05-19 Iiiumina Cambridge Limited Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
SG11202109768PA (en) * 2019-12-02 2021-10-28 Illumina Cambridge Ltd Time-based cluster imaging of amplified contiguity preserved liarary fragments of genomic dna
CN115349128A (zh) 2020-02-13 2022-11-15 齐默尔根公司 宏基因组文库和天然产物发现平台
WO2021232184A1 (zh) * 2020-05-18 2021-11-25 深圳华大智造科技有限公司 标签化的转座复合体及其在高通量测序中的应用
CA3198842A1 (en) * 2020-10-21 2022-04-28 Illumina, Inc. Sequencing templates comprising multiple inserts and compositions and methods for improving sequencing throughput
CN112795563A (zh) * 2021-03-23 2021-05-14 上海欣百诺生物科技有限公司 生物素化的转座体在回收CUT&Tag或ATAC-seq产物中的用途及方法
WO2023225519A1 (en) 2022-05-17 2023-11-23 10X Genomics, Inc. Modified transposons, compositions and uses thereof
CN115747301B (zh) * 2022-08-01 2023-12-22 深圳赛陆医疗科技有限公司 一种测序文库的构建方法、构建测序文库的试剂盒及基因测序方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
CA2643700A1 (en) * 2006-02-24 2007-11-22 Callida Genomics, Inc. High throughput genome sequencing on dna arrays
EP3272879B1 (en) * 2008-10-24 2019-08-07 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CA2803693A1 (en) * 2010-08-27 2012-03-01 Genentech, Inc. Methods for nucleic acid capture and sequencing
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
WO2012048341A1 (en) * 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
EP2635679B1 (en) * 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US8829171B2 (en) * 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
DK3037536T3 (da) * 2011-01-28 2020-01-13 Illumina Inc Oligonukleotiderstatning for di-taggede og retnings biblioteker
EP2670894B1 (en) * 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
NO2694769T3 (pt) * 2012-03-06 2018-03-03
SG11201405669XA (en) 2012-03-13 2014-10-30 Swift Biosciences Inc Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
US9644199B2 (en) * 2012-10-01 2017-05-09 Agilent Technologies, Inc. Immobilized transposase complexes for DNA fragmentation and tagging
US9683230B2 (en) * 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10550426B2 (en) * 2013-03-07 2020-02-04 Sekisui Medical Co., Ltd. Method for detecting methylated DNA
DK2970951T3 (da) * 2013-03-13 2019-05-13 Illumina Inc Fremgangsmåder til nukleinsyresekventering
AU2014312208B2 (en) * 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
PL3089822T3 (pl) * 2013-12-30 2022-09-19 Atreca, Inc. Analiza kwasów nukleinowych powiązanych z pojedynczymi komórkami z użyciem kodów kreskowych kwasów nukleinowych

Also Published As

Publication number Publication date
CN107969137A (zh) 2018-04-27
DK3207134T3 (da) 2019-09-23
CN107969137B (zh) 2021-10-26
EP3207134B1 (en) 2019-07-03
CN114045282A (zh) 2022-02-15
EP3207134A2 (en) 2017-08-23
EP3636757A1 (en) 2020-04-15
LT3207134T (lt) 2019-09-10
ES2745814T3 (es) 2020-03-03

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