ES2848829T3 - Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos - Google Patents

Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos Download PDF

Info

Publication number
ES2848829T3
ES2848829T3 ES18150783T ES18150783T ES2848829T3 ES 2848829 T3 ES2848829 T3 ES 2848829T3 ES 18150783 T ES18150783 T ES 18150783T ES 18150783 T ES18150783 T ES 18150783T ES 2848829 T3 ES2848829 T3 ES 2848829T3
Authority
ES
Spain
Prior art keywords
amplification
nucleic acids
target nucleic
sites
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES18150783T
Other languages
English (en)
Spanish (es)
Inventor
Jonathan Mark Boutell
M Shane Bowen
Kevin L Gunderson
Mostafa Ronaghi
Min-Jui Richard Shen
Kathryn M Stephens
Bala Murali Venkatesan
Kandaswamy Vijayan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49756442&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2848829(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Illumina Inc filed Critical Illumina Inc
Application granted granted Critical
Publication of ES2848829T3 publication Critical patent/ES2848829T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B01J2219/00587—High throughput processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00608—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00621—Delimitation of the attachment areas by physical means, e.g. trenches, raised areas
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00675—In-situ synthesis on the substrate
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/513—Detection characterised by immobilisation to a surface characterised by the pattern of the arrayed oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/537—Detection characterised by immobilisation to a surface characterised by the capture oligonucleotide acting as a primer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
ES18150783T 2012-06-15 2013-06-12 Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos Active ES2848829T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261660487P 2012-06-15 2012-06-15
US201261715478P 2012-10-18 2012-10-18
US13/783,043 US8895249B2 (en) 2012-06-15 2013-03-01 Kinetic exclusion amplification of nucleic acid libraries

Publications (1)

Publication Number Publication Date
ES2848829T3 true ES2848829T3 (es) 2021-08-12

Family

ID=49756442

Family Applications (2)

Application Number Title Priority Date Filing Date
ES18150783T Active ES2848829T3 (es) 2012-06-15 2013-06-12 Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos
ES13731229.4T Active ES2662098T3 (es) 2012-06-15 2013-06-12 Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES13731229.4T Active ES2662098T3 (es) 2012-06-15 2013-06-12 Amplificación de exclusión cinética de bibliotecas de ácidos nucleicos

Country Status (12)

Country Link
US (6) US8895249B2 (enExample)
EP (2) EP3366781B1 (enExample)
JP (3) JP6014249B2 (enExample)
KR (2) KR101954343B1 (enExample)
CN (2) CN107570092B (enExample)
AU (3) AU2013274283B2 (enExample)
BR (1) BR112014024897B1 (enExample)
CA (1) CA2867716C (enExample)
DK (2) DK2861756T3 (enExample)
ES (2) ES2848829T3 (enExample)
IL (2) IL234865B (enExample)
WO (1) WO2013188582A1 (enExample)

Families Citing this family (212)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US9309566B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid amplification
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
JP6017458B2 (ja) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
CN104105797B (zh) 2011-12-01 2016-08-31 吉纳普赛斯股份有限公司 用于高效电子测序与检测的系统和方法
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
EP2971141B1 (en) 2013-03-15 2018-11-28 Genapsys, Inc. Systems for biological analysis
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
EP3038834B1 (en) 2013-08-30 2018-12-12 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US10540783B2 (en) 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
EP3080300B1 (en) 2013-12-11 2020-09-02 Genapsys Inc. Systems and methods for biological analysis and computation
EP3084523B1 (en) 2013-12-19 2019-07-03 Illumina, Inc. Substrates comprising nano-patterning surfaces and methods of preparing thereof
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
EP3132060B1 (en) 2014-04-18 2019-03-13 Genapsys Inc. Methods and systems for nucleic acid amplification
SG11201610357UA (en) * 2014-06-13 2017-01-27 Illumina Cambridge Ltd Methods and compositions for preparing sequencing libraries
GB201416422D0 (en) * 2014-09-17 2014-10-29 Illumina Cambridge Ltd Flexible tape-based chemistry apparatus
JP2017529093A (ja) * 2014-09-29 2017-10-05 イルミナ ケンブリッジ リミテッド リコンビナーゼ変異体
BR122021026779B1 (pt) 2014-10-17 2023-12-19 Illumina Cambridge Limited Transposon de preservação de contiguidade
PL3218511T3 (pl) * 2014-11-11 2020-10-19 Illumina Cambridge Limited Sposoby i macierze do wytwarzania i sekwencjonowania monoklonalnych klastrów kwasu nukleinowego
PT3234187T (pt) 2014-12-15 2021-05-26 Illumina Inc Método para colocação de moleculas de alvo unico num substrato
EP4321627A3 (en) 2015-04-10 2024-04-17 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
EP3283870B1 (en) 2015-04-14 2020-05-06 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
CN107810415B (zh) 2015-05-11 2021-06-22 亿明达股份有限公司 用于发现和分析治疗剂的平台
EP3303614B1 (en) * 2015-05-29 2020-03-11 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
IL294145B2 (en) * 2015-07-30 2024-02-01 Illumina Inc Removal of orthogonal blocking of nucleotides
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
JP2019508669A (ja) 2016-01-11 2019-03-28 イラミーナ インコーポレーテッド マイクロフルオロメータ、流体システム、およびフローセルラッチクランプモジュールを有する検出装置
SG11201805665UA (en) 2016-03-28 2018-10-30 Illumina Inc Multi-plane microarrays
WO2017172699A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Recombinase mutants
EP3440220B1 (en) 2016-04-07 2020-03-25 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
US11078475B2 (en) 2016-05-03 2021-08-03 Sinai Health System Methods of capturing cell-free methylated DNA and uses of same
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. SYSTEMS AND METHODS FOR SEQUENCING NUCLEIC ACIDS
WO2018018008A1 (en) 2016-07-22 2018-01-25 Oregon Health & Science University Single cell whole genome libraries and combinatorial indexing methods of making thereof
GB201704754D0 (en) * 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
CN109414673B (zh) 2017-02-01 2021-09-07 伊鲁米那股份有限公司 具有响应于多个激发频率的基准的系统和方法
US20200102587A1 (en) 2017-04-05 2020-04-02 Hitachi High-Technologies Corporation Nucleic acid amplification method and nucleic acid analyzer
CN111094584B (zh) 2017-04-23 2024-11-26 伊鲁米那股份有限公司 用于改进编索引的核酸文库中的样品鉴定的组合物和方法
WO2018197950A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
US12584169B2 (en) 2017-04-23 2026-03-24 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3842545B1 (en) 2017-04-23 2022-11-30 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
WO2018197945A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CN107254572B (zh) * 2017-06-01 2019-07-02 首钢集团有限公司 一种冷轧硅锰双相钢表面麻点缺陷的控制方法
ES3014783T3 (en) 2017-06-07 2025-04-24 Univ Oregon Health & Science Single cell whole genome libraries for methylation sequencing
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. SEQUENCING SYSTEM COMPRISING AGGREGATION OF MULTIPLEXED BIOLOGICAL SAMPLES
CN119842876A (zh) 2017-08-01 2025-04-18 伊鲁米纳公司 使用的水凝胶珠和流动池的遗传物质的空间索引和文库制备
US11007523B2 (en) 2017-09-01 2021-05-18 Mgi Tech Co., Ltd. Injection molded microfluidic/fluidic cartridge integrated with silicon-based sensor
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. NUCLEIC ACID SEQUENCING SYSTEMS AND METHODS
CN107622185B (zh) * 2017-10-27 2020-08-21 领航基因科技（杭州）有限公司 一种数字pcr浓度计算方法
CA3089756A1 (en) * 2018-01-29 2019-08-01 St. Jude Children's Research Hospital, Inc. Method for nucleic acid amplification
CN110832086A (zh) 2018-04-02 2020-02-21 伊鲁米那股份有限公司 用于制造用于基于序列的遗传检验的对照的组合物和方法
RU2744175C1 (ru) 2018-05-17 2021-03-03 Иллумина, Инк. Высокопроизводительное секвенирование одиночной клетки со сниженной ошибкой амплификации
CA3253118A1 (en) 2018-06-04 2026-03-02 Illumina, Inc. High-throughput single-cell transcriptome libraries and methods of making and of using
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN113767175B (zh) 2018-08-28 2025-07-25 10X基因组学股份有限公司 增加空间阵列分辨率
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
AU2019391274B2 (en) 2018-12-05 2025-10-30 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
EP3894585A2 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Generating capture probes for spatial analysis
CN112739830A (zh) * 2018-12-18 2021-04-30 伊卢米纳剑桥有限公司 使用单一表面引物进行配对末端测序的方法和组合物
BR112020026667A2 (pt) 2018-12-19 2021-07-27 Illumina, Inc. métodos para melhorar a prioridade de clonalidade do agrupamento de polinucleotídeo
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
CA3129315A1 (en) * 2019-02-11 2020-08-20 Ultima Genomics, Inc. Methods for nucleic acid analysis
WO2020180778A1 (en) 2019-03-01 2020-09-10 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2021021515A1 (en) 2019-08-01 2021-02-04 Illumina, Inc. Flow cells
CN110694599A (zh) * 2019-09-26 2020-01-17 浙江海洋大学 一种聚丙烯亚胺修饰的磁性纳晶纤维素分子印迹聚合物的制备方法
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
WO2021127436A2 (en) 2019-12-19 2021-06-24 Illumina, Inc. High-throughput single-cell libraries and methods of making and of using
EP4752521A3 (en) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixing reagents and methods of use thereof
EP4081656A1 (en) 2019-12-23 2022-11-02 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
WO2021142769A1 (zh) * 2020-01-17 2021-07-22 深圳华大智造科技有限公司 同步进行dna正义链和反义链测序的方法
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11408032B2 (en) 2020-01-17 2022-08-09 Element Biosciences, Inc. Tube lens design for improved depth-of-field
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US20220316003A1 (en) 2020-03-09 2022-10-06 Illumina, Inc. Methods for sequencing polynucleotides
WO2021180724A1 (en) 2020-03-09 2021-09-16 Illumina, Inc. Improvements in nucleic acid sequencing
BR112022019541A2 (pt) 2020-03-30 2022-11-16 Illumina Inc Métodos e composições para preparação de bibliotecas de ácido nucleico
EP4139485B1 (en) 2020-04-22 2023-09-06 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
US12209266B2 (en) 2020-05-12 2025-01-28 Illumina, Inc. Generating nucleic acids with modified bases using recombinant terminal deoxynucleotidyl transferase
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
EP4153775B1 (en) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (en) 2020-06-08 2025-08-13 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
KR20230020977A (ko) 2020-06-09 2023-02-13 일루미나, 인코포레이티드 시퀀싱 라이브러리의 생성을 증가시키는 방법
EP4446430A3 (en) 2020-06-10 2024-12-18 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
US12592321B2 (en) 2020-06-19 2026-03-31 University Health Network Cancer detection and classification using methylome analysis
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
JP2023532231A (ja) 2020-06-30 2023-07-27 イルミナ インコーポレイテッド 傷なしｄｎａを生成するための触媒的に制御された合成による配列決定
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
MX2023000345A (es) 2020-07-08 2023-02-13 Illumina Inc Globulos como portadores de transposomas.
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
MX2022014724A (es) 2020-09-14 2023-03-06 Illumina Inc Composiciones y metodos para amplificar polinucleótidos.
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
MX2023007842A (es) 2021-01-08 2023-09-19 Cellanome Inc Dispositivos y metodos para analizar muestras biologicas.
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
US12576399B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for imaging and analyzing a cell in a compartment of a fluidic device
EP4284944A1 (en) * 2021-01-29 2023-12-06 Illumina, Inc. Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
US20220333178A1 (en) 2021-03-22 2022-10-20 Illumina Cambridge Limited Methods for improving nucleic acid cluster clonality
CA3212595A1 (en) 2021-04-13 2022-10-20 Sebastien RICOULT Compositions, systems, and methods of making and using encapsulated lyophilised microspheres
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
WO2022272260A1 (en) * 2021-06-23 2022-12-29 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
EP4359122A1 (en) 2021-06-25 2024-05-01 Illumina, Inc. Fiducials for use in registration of a patterned surface
EP4359129A1 (en) 2021-06-25 2024-05-01 Illumina, Inc. Linear fourier fiducial
EP4679300A3 (en) 2021-07-19 2026-04-22 Illumina, Inc. Intensity extraction with interpolation and adaptation for base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
GB202110479D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
US20250269368A1 (en) 2021-07-21 2025-08-28 Dnae Group Holdings Limited Method and system comprising a cartridge for sequencing target polynucleotides
CN119061125A (zh) 2021-07-21 2024-12-03 元素生物科学公司 用于核酸测序的方法
GB202110485D0 (en) 2021-07-21 2021-09-01 Dnae Diagnostics Ltd Compositions, kits and methods for sequencing target polynucleotides
EP4373958A1 (en) * 2021-07-23 2024-05-29 Illumina, Inc. Methods for preparing substrate surface for dna sequencing
EP4344443A1 (en) 2021-08-31 2024-04-03 Illumina, Inc. Flow cell with enhanced well imaging resolution
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023052629A2 (en) 2021-09-30 2023-04-06 Illumina Cambridge Limited Blocker methods
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
CA3223595A1 (en) 2021-12-16 2023-06-22 Xiaoyu Ma Hybrid clustering
AU2022424380A1 (en) 2021-12-29 2024-01-18 Illumina, Inc. Methods of nucleic acid sequencing using surface-bound primers
WO2023175040A2 (en) 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides for methylation detection
JP2025509651A (ja) 2022-03-15 2025-04-11 イルミナ インコーポレイテッド 並行サンプル及びインデックス配列決定
US12661647B2 (en) 2022-03-22 2026-06-23 Cellanome, Inc. Methods for enclosing and analyzing a cell in a fluidic device
AU2023244497A1 (en) 2022-03-31 2024-01-18 Illumina, Inc. Paired-end re-synthesis using blocked p5 primers
AU2023248405A1 (en) 2022-04-07 2024-01-04 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20240033738A1 (en) 2022-07-27 2024-02-01 Illumina, Inc. Flow cell based motion system calibration and control methods
JP2025530615A (ja) * 2022-08-26 2025-09-17 イルミナ インコーポレイテッド パターン化された結合領域を有するフローセル
WO2024061799A1 (en) 2022-09-19 2024-03-28 Illumina, Inc. Deformable polymers comprising immobilised primers
US20240100518A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Flow cell based motion system calibration and control methods
US20240102067A1 (en) 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods
US20240124929A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Mesophilic compositions for nucleic acid amplification
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240110221A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of modulating clustering kinetics
US20240110234A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Amplification Compositions and Methods
US20250388894A1 (en) 2022-09-30 2025-12-25 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
US20240124914A1 (en) 2022-09-30 2024-04-18 Illumina, Inc. Thermophilic compositions for nucleic acid amplification
EP4594481A1 (en) 2022-09-30 2025-08-06 Illumina, Inc. Helicase-cytidine deaminase complexes and methods of use
GB202215624D0 (en) 2022-10-21 2022-12-07 Dnae Diagnostics Ltd Clonal amplification
EP4685239A1 (en) 2022-11-09 2026-01-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4627115A1 (en) 2022-11-29 2025-10-08 Element Biosciences, Inc. Flow cell devices and optical systems for nucleic acid sequencing
CA3246533A1 (en) 2022-12-07 2024-06-13 Illumina, Inc. STERIC EXCLUSION MONOCLONAL GROUPING OF DOUBLE-STRANDED DNA WITH PATTERNED ASSEMBLY
US20240209419A1 (en) 2022-12-14 2024-06-27 Illumina, Inc. Systems and Methods for Capture and Enrichment of Clustered Beads on Flow Cell Substrates
WO2024249200A1 (en) 2023-05-26 2024-12-05 Illumina, Inc. Methods for preserving methylation status during clustering
US20240401129A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Methods for double-stranded sequencing by synthesis
WO2024249061A1 (en) 2023-05-31 2024-12-05 Illumina, Inc. Detecting a methylated nucleotide using a quencher
EP4728092A1 (en) 2023-06-14 2026-04-22 Illumina, Inc. Determination of modified cytosines
WO2024256580A1 (en) 2023-06-14 2024-12-19 Illumina, Inc. Concurrent sequencing with spatially separated rings
EP4780981A1 (en) 2023-09-20 2026-07-29 Illumina, Inc. Concurrent sequencing using nick translation
WO2025062001A1 (en) 2023-09-20 2025-03-27 Illumina, Inc. Optimised nucleic acid sequencing
EP4784740A1 (en) 2023-09-28 2026-08-05 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2025072683A1 (en) 2023-09-29 2025-04-03 Illumina, Inc. Solid supports and methods for strand invasion-based amplification
WO2025081064A2 (en) 2023-10-11 2025-04-17 Illumina, Inc. Thermophilic deaminase and methods for identifying modified cytosine
WO2025137222A1 (en) 2023-12-19 2025-06-26 Illumina, Inc. Methylation detection assay
WO2025137479A1 (en) 2023-12-22 2025-06-26 Illumina, Inc. Reusable flow cells and methods of using them for nucleic acid sequencing
WO2025207535A1 (en) 2024-03-26 2025-10-02 Illumina, Inc. Photo-switchable surfaces
WO2025240905A1 (en) 2024-05-17 2025-11-20 The Broad Institute, Inc. Imaging-free high-resolution spatial macromolecule abundance reconstruction
WO2025264836A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2025264831A1 (en) 2024-06-18 2025-12-26 Illumina, Inc. Methods for increasing sequencing quality of gc-rich regions
WO2026006746A2 (en) 2024-06-28 2026-01-02 Illumina, Inc. Nucleic acid preparation and analysis techniques
WO2026006314A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Tagging target regions prior to nucleotide sequencing
WO2026006774A1 (en) 2024-06-28 2026-01-02 Illumina, Inc. Altered cytidine deaminases and methods of use
WO2026090042A1 (en) 2024-10-21 2026-04-30 The Broad Institute, Inc. Single-cell and spatial dna methylation measurements
WO2026097017A2 (en) 2024-11-01 2026-05-07 Illumina, Inc. Methods of improving sequencing accuracy
WO2026128365A1 (en) 2024-12-09 2026-06-18 Illumina, Inc. Methods for increasing sequencing quality
WO2026142995A1 (en) 2024-12-26 2026-07-02 Illumina, Inc. Methods for producing singe-cell sequencing libraries of dna originating from open chromatin
WO2026142884A1 (en) 2024-12-27 2026-07-02 Illumina, Inc. Methods and compositions for identifying tissue permeabilization conditions for producing spatial sequencing libraries

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US169513A (en) 1875-11-02 Improvement in electric telegraphs
US4787963A (en) 1987-05-04 1988-11-29 Syntro Corporation Method and means for annealing complementary nucleic acid molecules at an accelerated rate
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
DE68926118T2 (de) 1988-08-18 1996-08-22 Australian Membrane And Biotechnology Research Institute Ltd. Commonwealth Scientific And Industrial Research Organization, North Ryde, Neusuedwales Verbesserungen an der empfindlichkeit und der selektivität von ionenkanalmembranbiosensoren
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2079005A1 (en) 1990-04-11 1991-10-12 Geoffrey Stephen Begg Methods and apparatus allowing sequential chemical reactions
US5223414A (en) 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5227265A (en) 1990-11-30 1993-07-13 Eastman Kodak Company Migration imaging system
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5605662A (en) 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
CA2185239C (en) 1994-03-16 2002-12-17 Nanibhushan Dattagupta Isothermal strand displacement nucleic acid amplification
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5728532A (en) * 1996-05-31 1998-03-17 Ackley; Donald E. Electrode configuration for matrix addressing of a molecular detection device
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
WO1998053296A1 (en) 1997-05-19 1998-11-26 Charles Evans And Associates Analysis of molecules bound to solid surfaces using selective bond cleavage processes
US6511803B1 (en) 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6699693B1 (en) 1999-02-04 2004-03-02 Sloan-Kettering Institute For Cancer Research Process for DNA replication
WO2000046408A1 (en) 1999-02-04 2000-08-10 Sloan-Kettering Institute For Cancer Research Process for dna replication
DE60040741D1 (de) 1999-04-20 2008-12-18 Illumina Inc Detektion von nukleinsäurereaktionen auf kügelchen-arrays
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050244870A1 (en) 1999-04-20 2005-11-03 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
JP2003521252A (ja) 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
EP2100971A3 (en) 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US6929915B2 (en) 2001-04-20 2005-08-16 The Penn State Research Foundation Methods for nucleic acid manipulation
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040002090A1 (en) 2002-03-05 2004-01-01 Pascal Mayer Methods for detecting genome-wide sequence variations associated with a phenotype
WO2003095664A2 (en) 2002-05-10 2003-11-20 City Of Hope Pyrophosphorolysis activated polymerization (pap)
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
DE602004026033D1 (de) 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
AU2004253882B2 (en) 2003-06-20 2010-06-10 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US8048627B2 (en) 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
EP2789383B1 (en) 2004-01-07 2023-05-03 Illumina Cambridge Limited Molecular arrays
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
EP2829615B1 (en) 2005-07-25 2018-05-09 Alere San Diego, Inc. Kit for multiplexing recombinase polymerase amplification
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
ES2416104T3 (es) 2006-03-07 2013-07-30 Cargill, Incorporated Aldolasas, ácidos nucleicos que las codifican y métodos para producir y usar las mismas
WO2007107710A1 (en) 2006-03-17 2007-09-27 Solexa Limited Isothermal methods for creating clonal single molecule arrays
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
US7702468B2 (en) * 2006-05-03 2010-04-20 Population Diagnostics, Inc. Evaluating genetic disorders
EP2029782B1 (en) 2006-05-04 2014-11-26 Alere San Diego, Inc. Recombinase polymerase amplification
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
US20080108804A1 (en) 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009012112A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
US8906831B2 (en) 2008-03-31 2014-12-09 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
US8945912B2 (en) * 2008-09-29 2015-02-03 The Board Of Trustees Of The University Of Illinois DNA sequencing and amplification systems using nanoscale field effect sensor arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US9309557B2 (en) 2010-12-17 2016-04-12 Life Technologies Corporation Nucleic acid amplification
US9334531B2 (en) * 2010-12-17 2016-05-10 Life Technologies Corporation Nucleic acid amplification
EP2454378B1 (en) * 2009-07-17 2015-12-23 Canon U.S. Life Sciences, Inc. Methods and systems for dna isolation on a microfluidic device
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
WO2011106368A2 (en) 2010-02-23 2011-09-01 Illumina, Inc. Amplification methods to minimise sequence specific bias
WO2011159942A1 (en) 2010-06-18 2011-12-22 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US20120083417A1 (en) * 2010-09-23 2012-04-05 Centrillion Technology Holding Corporation Native-extension parallel sequencing
EP2625526B1 (en) 2010-10-04 2017-03-15 Genapsys Inc. Systems and methods for automated reusable parallel biological reactions
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US20130344540A1 (en) * 2011-02-03 2013-12-26 Mark T. Reed Methods for minimizing sequence specific bias
CN102212621B (zh) * 2011-05-09 2014-01-08 中国人民解放军第四军医大学 一种用于乙型肝炎病毒基因分型的检测试剂盒及检测方法
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
CA2856163C (en) 2011-10-28 2019-05-07 Illumina, Inc. Microarray fabrication system and method
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) * 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries

Also Published As

Publication number Publication date
BR112014024897A2 (pt) 2017-06-20
ES2662098T3 (es) 2018-04-05
EP2861756A1 (en) 2015-04-22
IL234865B (en) 2020-10-29
AU2018253621A1 (en) 2018-11-22
US9758816B2 (en) 2017-09-12
EP3366781A1 (en) 2018-08-29
AU2013274283A1 (en) 2014-10-09
US11254976B2 (en) 2022-02-22
JP6339143B2 (ja) 2018-06-06
CN107570092A (zh) 2018-01-12
EP3366781B1 (en) 2020-10-28
JP2015519084A (ja) 2015-07-09
US20170335380A1 (en) 2017-11-23
JP2018139607A (ja) 2018-09-13
HK1253573A1 (en) 2019-06-21
CN104379766B (zh) 2017-09-26
DK2861756T3 (en) 2018-04-09
US20220243261A1 (en) 2022-08-04
CA2867716C (en) 2017-11-28
EP2861756B1 (en) 2018-01-10
IL278008B (en) 2021-12-01
BR112014024897B1 (pt) 2021-10-05
JP6014249B2 (ja) 2016-10-25
AU2021202677B2 (en) 2023-06-22
DK3366781T3 (da) 2021-02-01
IL234865A0 (en) 2014-12-31
CN104379766A (zh) 2015-02-25
US20200040386A1 (en) 2020-02-06
KR101757668B1 (ko) 2017-07-14
US8895249B2 (en) 2014-11-25
AU2013274283B2 (en) 2018-08-09
KR101954343B1 (ko) 2019-03-05
US9169513B2 (en) 2015-10-27
CN107570092B (zh) 2020-03-17
US12503726B2 (en) 2025-12-23
US10385384B2 (en) 2019-08-20
US20160053310A1 (en) 2016-02-25
HK1204012A1 (en) 2015-11-06
KR20140140083A (ko) 2014-12-08
WO2013188582A1 (en) 2013-12-19
IL278008A (en) 2020-11-30
AU2021202677A1 (en) 2021-05-27
CA2867716A1 (en) 2013-12-19
US20130338042A1 (en) 2013-12-19
JP2017060465A (ja) 2017-03-30
US20150080230A1 (en) 2015-03-19
KR20170083643A (ko) 2017-07-18
JP6585767B2 (ja) 2019-10-02

Similar Documents

Publication Publication Date Title
US12503726B2 (en) Kinetic exclusion amplification of nucleic acid libraries
ES3030504T3 (en) Kinetic exclusion amplification of nucleic acid libraries
HK1253573B (en) Kinetic exclusion amplification of nucleic acid libraries
HK1204012B (en) Kinetic exclusion amplification of nucleic acid libraries
HK40015843A (en) Kinetic exclusion amplification of nucleic acid libraries
HK40015843B (en) Kinetic exclusion amplification of nucleic acid libraries