JP2017538435A5 - - Google Patents
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- Publication number
- JP2017538435A5 JP2017538435A5 JP2017533539A JP2017533539A JP2017538435A5 JP 2017538435 A5 JP2017538435 A5 JP 2017538435A5 JP 2017533539 A JP2017533539 A JP 2017533539A JP 2017533539 A JP2017533539 A JP 2017533539A JP 2017538435 A5 JP2017538435 A5 JP 2017538435A5
- Authority
- JP
- Japan
- Prior art keywords
- medium
- poloxamer
- concentration
- liquid medium
- hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002609 medium Substances 0.000 claims 47
- CTKXFMQHOOWWEB-UHFFFAOYSA-N Ethylene oxide/propylene oxide copolymer Chemical compound CCCOC(C)COCCO CTKXFMQHOOWWEB-UHFFFAOYSA-N 0.000 claims 27
- 238000000034 method Methods 0.000 claims 27
- 229940044519 poloxamer 188 Drugs 0.000 claims 27
- 229920001993 poloxamer 188 Polymers 0.000 claims 27
- 239000007788 liquid Substances 0.000 claims 24
- 210000004027 cell Anatomy 0.000 claims 17
- 239000011148 porous material Substances 0.000 claims 12
- 238000012258 culturing Methods 0.000 claims 10
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 9
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 9
- 210000004962 mammalian cell Anatomy 0.000 claims 8
- 239000001963 growth medium Substances 0.000 claims 4
- 238000009630 liquid culture Methods 0.000 claims 4
- 230000010412 perfusion Effects 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 3
- 108090000623 proteins and genes Proteins 0.000 claims 3
- 238000013019 agitation Methods 0.000 claims 2
- 239000002518 antifoaming agent Substances 0.000 claims 2
- 210000003734 kidney Anatomy 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 239000008194 pharmaceutical composition Substances 0.000 claims 2
- 241000699800 Cricetinae Species 0.000 claims 1
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 108060003951 Immunoglobulin Proteins 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 102000018358 immunoglobulin Human genes 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 239000002304 perfume Substances 0.000 claims 1
- 210000002966 serum Anatomy 0.000 claims 1
- 210000003501 vero cell Anatomy 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462095734P | 2014-12-22 | 2014-12-22 | |
| US62/095,734 | 2014-12-22 | ||
| PCT/US2015/067040 WO2016106192A2 (en) | 2014-12-22 | 2015-12-21 | Methods of culturing a mammalian cell |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177700A Division JP7075463B2 (ja) | 2014-12-22 | 2020-10-23 | 哺乳類細胞の培養方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017538435A JP2017538435A (ja) | 2017-12-28 |
| JP2017538435A5 true JP2017538435A5 (esLanguage) | 2019-01-31 |
| JP6843750B2 JP6843750B2 (ja) | 2021-03-17 |
Family
ID=55527619
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017533539A Active JP6843750B2 (ja) | 2014-12-22 | 2015-12-21 | 哺乳類細胞の培養方法 |
| JP2020177700A Active JP7075463B2 (ja) | 2014-12-22 | 2020-10-23 | 哺乳類細胞の培養方法 |
| JP2022079207A Pending JP2022107000A (ja) | 2014-12-22 | 2022-05-13 | 哺乳類細胞の培養方法 |
| JP2024014536A Pending JP2024050754A (ja) | 2014-12-22 | 2024-02-02 | 哺乳類細胞の培養方法 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177700A Active JP7075463B2 (ja) | 2014-12-22 | 2020-10-23 | 哺乳類細胞の培養方法 |
| JP2022079207A Pending JP2022107000A (ja) | 2014-12-22 | 2022-05-13 | 哺乳類細胞の培養方法 |
| JP2024014536A Pending JP2024050754A (ja) | 2014-12-22 | 2024-02-02 | 哺乳類細胞の培養方法 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US11306341B2 (esLanguage) |
| EP (1) | EP3237604B1 (esLanguage) |
| JP (4) | JP6843750B2 (esLanguage) |
| KR (3) | KR20250025516A (esLanguage) |
| CN (1) | CN107567495A (esLanguage) |
| AU (3) | AU2015369809B2 (esLanguage) |
| BR (1) | BR112017013423B1 (esLanguage) |
| CA (2) | CA3225136A1 (esLanguage) |
| HK (1) | HK1246345A1 (esLanguage) |
| IL (2) | IL308064A (esLanguage) |
| MX (2) | MX383857B (esLanguage) |
| RU (1) | RU2712746C2 (esLanguage) |
| SG (2) | SG10202001034PA (esLanguage) |
| TW (3) | TWI751103B (esLanguage) |
| WO (1) | WO2016106192A2 (esLanguage) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2958988A2 (en) | 2013-02-22 | 2015-12-30 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| US10421949B2 (en) | 2013-02-22 | 2019-09-24 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| TWI743024B (zh) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
| TWI776235B (zh) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| WO2016106192A2 (en) | 2014-12-22 | 2016-06-30 | Genzyme Corporation | Methods of culturing a mammalian cell |
| SG11202009047TA (en) * | 2018-03-16 | 2020-10-29 | Genzyme Corp | Methods for improving cell viability in a production bioreactor |
| US12399100B2 (en) | 2020-08-12 | 2025-08-26 | Allen Institute | Systems, devices, and methods for quality control of cell production using predictive tagging |
| CN112795531B (zh) * | 2021-04-11 | 2021-08-06 | 依科赛生物科技（太仓）有限公司 | 一种cho细胞无血清无蛋白培养基及其用途 |
| EP4410957A4 (en) * | 2021-09-30 | 2026-01-28 | Fujifilm Corp | CELL CULTURE PROCESS AND PROCESS FOR PRODUCING USEFUL SUBSTANCE |
| US12157873B2 (en) | 2022-09-28 | 2024-12-03 | Genzyme Corporation | Systems and devices for producing biological products and methods of using the same |
Family Cites Families (48)
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| US4522811A (en) | 1982-07-08 | 1985-06-11 | Syntex (U.S.A.) Inc. | Serial injection of muramyldipeptides and liposomes enhances the anti-infective activity of muramyldipeptides |
| US5658782A (en) | 1993-10-20 | 1997-08-19 | State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University A Non-Profit Organization | Amino acid transporters and uses |
| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| US5721121A (en) | 1995-06-06 | 1998-02-24 | Genentech, Inc. | Mammalian cell culture process for producing a tumor necrosis factor receptor immunoglobulin chimeric protein |
| US6458574B1 (en) * | 1996-09-12 | 2002-10-01 | Transkaryotic Therapies, Inc. | Treatment of a α-galactosidase a deficiency |
| AU2002232726A1 (en) | 2000-12-20 | 2002-07-01 | Bayer Pharmaceuticals Corporation | A device and method for seed-train expansion of mammalian cells |
| EP1404813A4 (en) | 2001-06-13 | 2004-11-24 | Genentech Inc | METHOD FOR CULTIVATING ANIMAL CELLS AND POLYPEPTIDE PRODUCTION IN ANIMAL CELLS |
| WO2003039459A2 (en) | 2001-11-05 | 2003-05-15 | Genvec, Inc. | Viral vector production methods and compositions |
| US6953692B2 (en) | 2001-12-18 | 2005-10-11 | Bayer Pharmaceuticals Corporation | Device and method for seed-train expansion of mammalian cells |
| US7067279B1 (en) * | 2002-08-23 | 2006-06-27 | Immunex Corporation | Cell culture performance with betaine |
| US6974681B1 (en) * | 2002-08-23 | 2005-12-13 | Immunex Corporation | Cell culture performance with vanadate |
| US20050186669A1 (en) | 2004-02-20 | 2005-08-25 | Cesco Bioengineering Co., Ltd. | Apparatus and method for preparing and culturing cells |
| ES2456015T3 (es) | 2004-03-05 | 2014-04-21 | Dsm Ip Assets B.V. | Procedimiento para cultivar células mediante perfusión continua y flujo tangencial alternante |
| WO2006033935A2 (en) | 2004-09-16 | 2006-03-30 | Becton, Dickinson And Company | Perfusion bioreactor for culturing cells |
| WO2006138143A1 (en) | 2005-06-15 | 2006-12-28 | Amprotein Corporation | Suspension culture vessels |
| JP2008543307A (ja) | 2005-06-15 | 2008-12-04 | アンプロテイン コーポレイション | 懸濁培養用容器 |
| WO2008024769A2 (en) * | 2006-08-21 | 2008-02-28 | Raven Biotechnologies, Inc. | Intensified perfusion production method |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| CA2842964A1 (en) | 2006-09-13 | 2008-03-20 | Abbvie Inc. | Cell culture improvements |
| US8501462B2 (en) | 2007-02-27 | 2013-08-06 | The Board Of Trustees Of The University Of Illinois | Insert device for multiwell plate |
| WO2009034186A2 (en) | 2007-09-13 | 2009-03-19 | Helmholtz-Zentrum für Infektionsforschung GmbH | Process for cell cultivation |
| EP2596795A1 (en) | 2007-11-08 | 2013-05-29 | Ambit Biosciences Corporation | Methods of administering n-(5-tert-butyl-isoxazol-3-yl)-n?-{4-[7-(2-morpholin-4-yl-ethoxy)imidazo[2,1-b][1,3]benzothiazol-2-yl]phenyl}urea to treat proliferative disease |
| BRPI0821467B8 (pt) * | 2007-12-31 | 2021-05-25 | Baxalta GmbH | composição de furina recombinante, método para produzir a composição de furina recombinante, e, método para produzir uma proteína madura utilizando a composição de furina recombinante |
| US20090233334A1 (en) * | 2008-03-11 | 2009-09-17 | Excellgene Sa | Cell cultivation and production of recombinant proteins by means of an orbital shake bioreactor system with disposable bags at the 1,500 liter scale |
| DE102008019691A1 (de) | 2008-04-15 | 2009-10-29 | Technische Universität Ilmenau | Teilaktives mikrofluidisches System für die 3D-Zellkultivierung sowie Verfahren zu dessen Perfusion |
| WO2010120514A2 (en) | 2009-03-31 | 2010-10-21 | The Trustees Of The University Of Pennsylvania | Antigen-binding proteins comprising recombinant protein scaffolds |
| KR101131867B1 (ko) | 2009-08-13 | 2012-04-03 | (주)히스토스템 | 폴리감마글루탐산을 포함하는 배양 세포 손상 방지를 위한 배지 첨가용 조성물 |
| PL2501822T3 (pl) * | 2009-11-17 | 2017-12-29 | E. R. Squibb & Sons, L.L.C. | Sposoby zwiększenia produkcji białek |
| WO2012152945A1 (en) | 2011-05-12 | 2012-11-15 | Probiogen Ag | Enhancement of protein production yield mediated by a fast shuttling cdc42 gtpase |
| WO2013116449A1 (en) | 2012-02-02 | 2013-08-08 | Corning Incorporated | Automatic continuous perfusion cell culture microplate consumables |
| EP2834649A4 (en) | 2012-04-01 | 2015-12-09 | Emd Millipore Corp | CELL CULTURE AND GRADIENT MIGRATION TEST METHOD AND DEVICES |
| MX363702B (es) | 2012-10-23 | 2019-03-29 | Genzyme Corp | Metodos de cultivo de perfusion y aplicaciones de los mismos. |
| EP2958988A2 (en) | 2013-02-22 | 2015-12-30 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| US10421949B2 (en) | 2013-02-22 | 2019-09-24 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| CA2902854C (en) | 2013-03-08 | 2024-01-23 | Genzyme Corporation | Integrated continuous manufacturing of therapeutic protein drug substances |
| US20140271633A1 (en) | 2013-03-14 | 2014-09-18 | Abbvie Inc. | Mammalian cell culture performance through surfactant supplementation of feed media |
| KR20210095233A (ko) | 2013-03-15 | 2021-07-30 | 젠자임 코포레이션 | 고-밀도 세포 뱅킹 방법 |
| EP3003325A4 (en) * | 2013-05-29 | 2017-01-11 | Biogen MA Inc. | Methods of evaluating cell culture additives |
| BR112016005545B1 (pt) | 2013-09-16 | 2021-06-01 | Genzyme Corporation | Método de processamento de uma cultura de células de mamífero |
| TWI709569B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析樹脂及其用於製造方法的用途 |
| TWI709570B (zh) | 2014-01-17 | 2020-11-11 | 美商健臻公司 | 無菌層析法及製法 |
| DK3926051T3 (da) * | 2014-06-04 | 2024-06-10 | Amgen Inc | Fremgangsmåder til høst af pattedyrecellekulturer |
| TWI743024B (zh) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
| TWI776235B (zh) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| WO2016106192A2 (en) | 2014-12-22 | 2016-06-30 | Genzyme Corporation | Methods of culturing a mammalian cell |
| JP6943972B2 (ja) | 2017-03-31 | 2021-10-06 | ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング | 灌流培地 |
| AU2020352651A1 (en) | 2019-09-27 | 2022-03-24 | Boehringer Ingelheim International Gmbh | Concentrated perfusion medium |
| TW202305109A (zh) | 2021-04-14 | 2023-02-01 | 美商健臻公司 | 灌注培養哺乳類動物細胞的方法 |
-
2015
- 2015-12-21 WO PCT/US2015/067040 patent/WO2016106192A2/en not_active Ceased
- 2015-12-21 IL IL308064A patent/IL308064A/en unknown
- 2015-12-21 KR KR1020257004730A patent/KR20250025516A/ko active Pending
- 2015-12-21 MX MX2017008338A patent/MX383857B/es unknown
- 2015-12-21 US US14/976,486 patent/US11306341B2/en active Active
- 2015-12-21 KR KR1020177020466A patent/KR102568951B1/ko active Active
- 2015-12-21 CA CA3225136A patent/CA3225136A1/en active Pending
- 2015-12-21 SG SG10202001034PA patent/SG10202001034PA/en unknown
- 2015-12-21 KR KR1020237027804A patent/KR102769649B1/ko active Active
- 2015-12-21 RU RU2017126045A patent/RU2712746C2/ru active
- 2015-12-21 HK HK18105611.8A patent/HK1246345A1/zh unknown
- 2015-12-21 IL IL253071A patent/IL253071B2/en unknown
- 2015-12-21 CA CA2971861A patent/CA2971861C/en active Active
- 2015-12-21 CN CN201580076640.3A patent/CN107567495A/zh active Pending
- 2015-12-21 SG SG11201705103WA patent/SG11201705103WA/en unknown
- 2015-12-21 JP JP2017533539A patent/JP6843750B2/ja active Active
- 2015-12-21 EP EP15843061.1A patent/EP3237604B1/en active Active
- 2015-12-21 BR BR112017013423-3A patent/BR112017013423B1/pt active IP Right Grant
- 2015-12-21 AU AU2015369809A patent/AU2015369809B2/en active Active
- 2015-12-22 TW TW104143177A patent/TWI751103B/zh active
- 2015-12-22 TW TW110131996A patent/TWI843012B/zh active
- 2015-12-22 TW TW109136823A patent/TWI741851B/zh active
-
2017
- 2017-06-21 MX MX2021007433A patent/MX2021007433A/es unknown
-
2020
- 2020-10-23 JP JP2020177700A patent/JP7075463B2/ja active Active
-
2022
- 2022-01-11 US US17/572,978 patent/US12486524B2/en active Active
- 2022-05-13 JP JP2022079207A patent/JP2022107000A/ja active Pending
- 2022-06-14 AU AU2022204152A patent/AU2022204152B2/en active Active
-
2024
- 2024-02-02 JP JP2024014536A patent/JP2024050754A/ja active Pending
-
2025
- 2025-02-27 AU AU2025201416A patent/AU2025201416A1/en active Pending
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