AU2015369809B2 - Methods of culturing a mammalian cell - Google Patents

Methods of culturing a mammalian cell Download PDF

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Publication number
AU2015369809B2
AU2015369809B2 AU2015369809A AU2015369809A AU2015369809B2 AU 2015369809 B2 AU2015369809 B2 AU 2015369809B2 AU 2015369809 A AU2015369809 A AU 2015369809A AU 2015369809 A AU2015369809 A AU 2015369809A AU 2015369809 B2 AU2015369809 B2 AU 2015369809B2
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Australia
Prior art keywords
concentration
cells
days
liquid medium
poloxamer
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AU2015369809A
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English (en)
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AU2015369809A1 (en
Inventor
Seul-A Bae
Cheng Cheng
Christopher HWANG
Timothy Johnson
Neha Shah
Jason Walther
Jonathan Wang
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Genzyme Corp
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Genzyme Corp
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Priority to AU2022204152A priority Critical patent/AU2022204152B2/en
Priority to AU2025201416A priority patent/AU2025201416A1/en
Active legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sustainable Development (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2015369809A 2014-12-22 2015-12-21 Methods of culturing a mammalian cell Active AU2015369809B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2022204152A AU2022204152B2 (en) 2014-12-22 2022-06-14 Methods of culturing a mammalian cell
AU2025201416A AU2025201416A1 (en) 2014-12-22 2025-02-27 Methods of culturing a mammalian cell

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462095734P 2014-12-22 2014-12-22
US62/095,734 2014-12-22
PCT/US2015/067040 WO2016106192A2 (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2022204152A Division AU2022204152B2 (en) 2014-12-22 2022-06-14 Methods of culturing a mammalian cell

Publications (2)

Publication Number Publication Date
AU2015369809A1 AU2015369809A1 (en) 2017-08-10
AU2015369809B2 true AU2015369809B2 (en) 2022-03-17

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AU2015369809A Active AU2015369809B2 (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell
AU2022204152A Active AU2022204152B2 (en) 2014-12-22 2022-06-14 Methods of culturing a mammalian cell
AU2025201416A Pending AU2025201416A1 (en) 2014-12-22 2025-02-27 Methods of culturing a mammalian cell

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AU2025201416A Pending AU2025201416A1 (en) 2014-12-22 2025-02-27 Methods of culturing a mammalian cell

Country Status (15)

Country Link
US (2) US11306341B2 (esLanguage)
EP (1) EP3237604B1 (esLanguage)
JP (4) JP6843750B2 (esLanguage)
KR (3) KR20250025516A (esLanguage)
CN (1) CN107567495A (esLanguage)
AU (3) AU2015369809B2 (esLanguage)
BR (1) BR112017013423B1 (esLanguage)
CA (2) CA3225136A1 (esLanguage)
HK (1) HK1246345A1 (esLanguage)
IL (2) IL308064A (esLanguage)
MX (2) MX383857B (esLanguage)
RU (1) RU2712746C2 (esLanguage)
SG (2) SG10202001034PA (esLanguage)
TW (3) TWI751103B (esLanguage)
WO (1) WO2016106192A2 (esLanguage)

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SG11202009047TA (en) * 2018-03-16 2020-10-29 Genzyme Corp Methods for improving cell viability in a production bioreactor
US12399100B2 (en) 2020-08-12 2025-08-26 Allen Institute Systems, devices, and methods for quality control of cell production using predictive tagging
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EP4410957A4 (en) * 2021-09-30 2026-01-28 Fujifilm Corp CELL CULTURE PROCESS AND PROCESS FOR PRODUCING USEFUL SUBSTANCE
US12157873B2 (en) 2022-09-28 2024-12-03 Genzyme Corporation Systems and devices for producing biological products and methods of using the same

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Publication number Publication date
JP7075463B2 (ja) 2022-05-25
AU2025201416A1 (en) 2025-03-20
IL253071B2 (en) 2024-04-01
EP3237604B1 (en) 2026-02-11
MX2017008338A (es) 2017-10-19
US11306341B2 (en) 2022-04-19
RU2020101112A (ru) 2020-03-13
RU2017126045A (ru) 2019-01-24
HK1246345A1 (zh) 2018-09-07
AU2022204152B2 (en) 2024-11-28
CA3225136A1 (en) 2016-06-30
US20160177361A1 (en) 2016-06-23
RU2712746C2 (ru) 2020-01-30
CA2971861A1 (en) 2016-06-30
KR102568951B1 (ko) 2023-08-22
CA2971861C (en) 2024-02-13
SG11201705103WA (en) 2017-07-28
IL253071A0 (en) 2017-08-31
TWI751103B (zh) 2022-01-01
KR20230125102A (ko) 2023-08-28
MX2021007433A (es) 2021-08-05
IL308064A (en) 2023-12-01
TW201639881A (zh) 2016-11-16
EP3237604A2 (en) 2017-11-01
BR112017013423B1 (pt) 2023-04-11
TWI843012B (zh) 2024-05-21
US12486524B2 (en) 2025-12-02
AU2022204152A1 (en) 2022-06-30
WO2016106192A3 (en) 2016-11-03
KR102769649B1 (ko) 2025-02-19
JP2021019624A (ja) 2021-02-18
RU2017126045A3 (esLanguage) 2019-06-24
AU2015369809A1 (en) 2017-08-10
JP2017538435A (ja) 2017-12-28
US20220145348A1 (en) 2022-05-12
TW202106706A (zh) 2021-02-16
BR112017013423A2 (pt) 2018-01-09
MX383857B (es) 2025-03-14
JP2022107000A (ja) 2022-07-20
KR20170090508A (ko) 2017-08-07
TWI741851B (zh) 2021-10-01
KR20250025516A (ko) 2025-02-21
WO2016106192A2 (en) 2016-06-30
CN107567495A (zh) 2018-01-09
JP2024050754A (ja) 2024-04-10
JP6843750B2 (ja) 2021-03-17
TW202200785A (zh) 2022-01-01
IL253071B1 (en) 2023-12-01
SG10202001034PA (en) 2020-04-29

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