BR112017013423A2 - métodos de cultura de célula de mamífero . - Google Patents

métodos de cultura de célula de mamífero .

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Publication number
BR112017013423A2
BR112017013423A2 BR112017013423A BR112017013423A BR112017013423A2 BR 112017013423 A2 BR112017013423 A2 BR 112017013423A2 BR 112017013423 A BR112017013423 A BR 112017013423A BR 112017013423 A BR112017013423 A BR 112017013423A BR 112017013423 A2 BR112017013423 A2 BR 112017013423A2
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BR
Brazil
Prior art keywords
mammalian cell
cell culture
culture methods
concentration
liquid medium
Prior art date
Application number
BR112017013423A
Other languages
English (en)
Other versions
BR112017013423B1 (pt
Inventor
Cheng Cheng
Hwang Christopher
WALTHER Jason
Wang Jonathan
SHAH Neha
Bae Seul-A
Johnson Timothy
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of BR112017013423A2 publication Critical patent/BR112017013423A2/pt
Publication of BR112017013423B1 publication Critical patent/BR112017013423B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0043—Medium free of human- or animal-derived components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/005—Protein-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01022—Alpha-galactosidase (3.2.1.22)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

a presente invenção refere-se aos métodos de cultura de uma célula de mamífero em um meio líquido incluindo poloxâmero-188 a uma concentração de 1,8 g / l, ou a uma concentração maior do que 1,8 g / l, ou um meio líquido que inclui uma concentração de poloxâmero-188, que é selecionada com base em um ou mais fatores selecionados a partir do grupo de: tamanho de poro, tipo de poro, taxa de fluxo de gás, densidade de célula viável no meio e marcadores relacionados ao estresse de célula.
BR112017013423-3A 2014-12-22 2015-12-21 Métodos para cultivo de célula de mamífero BR112017013423B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462095734P 2014-12-22 2014-12-22
US62/095,734 2014-12-22
PCT/US2015/067040 WO2016106192A2 (en) 2014-12-22 2015-12-21 Methods of culturing a mammalian cell

Publications (2)

Publication Number Publication Date
BR112017013423A2 true BR112017013423A2 (pt) 2018-01-09
BR112017013423B1 BR112017013423B1 (pt) 2023-04-11

Family

ID=55527619

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017013423-3A BR112017013423B1 (pt) 2014-12-22 2015-12-21 Métodos para cultivo de célula de mamífero

Country Status (15)

Country Link
US (2) US11306341B2 (pt)
EP (1) EP3237604A2 (pt)
JP (4) JP6843750B2 (pt)
KR (2) KR20230125102A (pt)
CN (1) CN107567495A (pt)
AU (2) AU2015369809B2 (pt)
BR (1) BR112017013423B1 (pt)
CA (2) CA3225136A1 (pt)
HK (1) HK1246345A1 (pt)
IL (2) IL253071B2 (pt)
MX (2) MX2017008338A (pt)
RU (1) RU2712746C2 (pt)
SG (2) SG10202001034PA (pt)
TW (2) TWI741851B (pt)
WO (1) WO2016106192A2 (pt)

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CA2901940A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
AU2014218723B2 (en) 2013-02-22 2019-12-05 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
CA3225136A1 (en) 2014-12-22 2016-06-30 Genzyme Corporation Methods of culturing a mammalian cell
CA3094076A1 (en) * 2018-03-16 2019-09-19 Genzyme Corporation Methods for improving cell viability in a production bioreactor
WO2022035973A1 (en) * 2020-08-12 2022-02-17 Allen Institute Systems, devices, and methods for quality control of cell production using predictive tagging
CN112795531B (zh) * 2021-04-11 2021-08-06 依科赛生物科技（太仓）有限公司 一种cho细胞无血清无蛋白培养基及其用途

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TW202204596A (zh) 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
TWI776235B (zh) 2014-06-09 2022-09-01 美商健臻公司 種子罐培養法(seed train processes)及其用途
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Also Published As

Publication number Publication date
SG10202001034PA (en) 2020-04-29
JP2022107000A (ja) 2022-07-20
IL308064A (en) 2023-12-01
TW202106706A (zh) 2021-02-16
CA3225136A1 (en) 2016-06-30
KR20230125102A (ko) 2023-08-28
MX2021007433A (es) 2021-08-05
RU2017126045A3 (pt) 2019-06-24
RU2020101112A (ru) 2020-03-13
RU2017126045A (ru) 2019-01-24
BR112017013423B1 (pt) 2023-04-11
AU2015369809B2 (en) 2022-03-17
TWI741851B (zh) 2021-10-01
WO2016106192A2 (en) 2016-06-30
CA2971861C (en) 2024-02-13
EP3237604A2 (en) 2017-11-01
MX2017008338A (es) 2017-10-19
HK1246345A1 (zh) 2018-09-07
IL253071A0 (en) 2017-08-31
KR102568951B1 (ko) 2023-08-22
AU2022204152A1 (en) 2022-06-30
JP2021019624A (ja) 2021-02-18
IL253071B2 (en) 2024-04-01
US20160177361A1 (en) 2016-06-23
JP7075463B2 (ja) 2022-05-25
US11306341B2 (en) 2022-04-19
SG11201705103WA (en) 2017-07-28
TW201639881A (zh) 2016-11-16
CN107567495A (zh) 2018-01-09
WO2016106192A3 (en) 2016-11-03
JP2024050754A (ja) 2024-04-10
AU2015369809A1 (en) 2017-08-10
JP6843750B2 (ja) 2021-03-17
KR20170090508A (ko) 2017-08-07
US20220145348A1 (en) 2022-05-12
TWI751103B (zh) 2022-01-01
TW202200785A (zh) 2022-01-01
JP2017538435A (ja) 2017-12-28
RU2712746C2 (ru) 2020-01-30
IL253071B1 (en) 2023-12-01
CA2971861A1 (en) 2016-06-30

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