JP2017510305A5 - - Google Patents
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- Publication number
- JP2017510305A5 JP2017510305A5 JP2017504620A JP2017504620A JP2017510305A5 JP 2017510305 A5 JP2017510305 A5 JP 2017510305A5 JP 2017504620 A JP2017504620 A JP 2017504620A JP 2017504620 A JP2017504620 A JP 2017504620A JP 2017510305 A5 JP2017510305 A5 JP 2017510305A5
- Authority
- JP
- Japan
- Prior art keywords
- water
- filter
- alcohol
- immiscible fluid
- wash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 9
- 239000012530 fluid Substances 0.000 claims 8
- 238000004140 cleaning Methods 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 238000005406 washing Methods 0.000 claims 5
- 230000005484 gravity Effects 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000003480 eluent Substances 0.000 claims 2
- 229920001477 hydrophilic polymer Polymers 0.000 claims 2
- 229920001410 Microfiber Polymers 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 239000003658 microfiber Substances 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461978322P | 2014-04-11 | 2014-04-11 | |
| US61/978,322 | 2014-04-11 | ||
| PCT/US2015/025335 WO2015157650A1 (en) | 2014-04-11 | 2015-04-10 | Nucleic acid purification method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017510305A JP2017510305A (ja) | 2017-04-13 |
| JP2017510305A5 true JP2017510305A5 (enExample) | 2018-06-07 |
| JP6562389B2 JP6562389B2 (ja) | 2019-08-21 |
Family
ID=53008879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017504620A Active JP6562389B2 (ja) | 2014-04-11 | 2015-04-10 | 核酸の精製方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20170029810A1 (enExample) |
| EP (1) | EP3129498B1 (enExample) |
| JP (1) | JP6562389B2 (enExample) |
| KR (1) | KR20160138578A (enExample) |
| CN (1) | CN106164270A (enExample) |
| ES (1) | ES2708205T3 (enExample) |
| WO (1) | WO2015157650A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2729113C2 (ru) * | 2014-11-21 | 2020-08-04 | Оккам Байолэбс, Инк. | Система и способ сбора образца нуклеиновой кислоты |
| PL413551A1 (pl) * | 2015-08-18 | 2017-02-27 | Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością | Sposób i system do izolacji kwasów nukleinowych |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| WO2019066363A2 (ko) * | 2017-09-27 | 2019-04-04 | 고려대학교 산학협력단 | 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템 |
| KR101941787B1 (ko) * | 2017-11-13 | 2019-04-12 | 고려대학교 산학협력단 | 타겟 물질을 추출하기 위한 추출 장치 |
| EP3925704A4 (en) * | 2019-02-14 | 2022-11-09 | Korea University Research and Business Foundation | EXTRACTION DEVICE, EXTRACTION METHOD AND FLUID CHIP FOR EXTRACTING TARGET MATERIAL |
| GB2581489B (en) * | 2019-02-15 | 2021-02-24 | Revolugen Ltd | Purification method |
| WO2021039540A1 (ja) | 2019-08-23 | 2021-03-04 | 富士フイルム和光純薬株式会社 | ニューモシスチス・イロベチイ検出用プライマー対、これを用いたニューモシスチス・イロベチイの検出方法及びそのための試薬キット |
| CN110724633A (zh) * | 2019-11-11 | 2020-01-24 | 浙江汇泽医药科技有限公司 | 一种微量细胞核酸提取与扩增系统及工艺 |
| KR20220098246A (ko) | 2019-12-18 | 2022-07-11 | 후지필름 가부시키가이샤 | 마이코박테륨·투베르쿨로시스, 마이코박테륨·아비움 및 마이코박테륨·인트라셀룰라레를 검출하기 위한 프라이머 세트 및 이것을 이용한 방법, 및 그를 위한 시약 키트 |
| WO2022030605A1 (ja) * | 2020-08-07 | 2022-02-10 | 株式会社ゴーフォトン | 核酸抽出容器および核酸抽出方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| JP4025399B2 (ja) * | 1997-10-28 | 2007-12-19 | 株式会社日立製作所 | 核酸の回収方法及び装置 |
| AU2001238616A1 (en) * | 2000-02-23 | 2001-09-03 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| US6706858B2 (en) * | 2001-05-11 | 2004-03-16 | Qiagen Gmbh | Use of alkanes for contamination-free purification or separation of biopolymers |
| EP3006934B1 (en) * | 2008-08-01 | 2017-02-01 | BioVentures, Inc., | Device for the purification, isolation, desalting or buffer/solvent exchange of substances |
| JP2013500742A (ja) * | 2009-08-04 | 2013-01-10 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 粒状物試料から微生物の核酸を回収するための方法およびデバイス |
| DK2611916T3 (en) * | 2010-09-02 | 2017-04-10 | Qiagen Gmbh | PROCEDURE FOR INSULATING A TARGET NUCLEIC ACID, INCLUDING SMALL TARGET ACIDS, WITH HIGH Yield |
| CN101935648B (zh) * | 2010-09-13 | 2013-04-17 | 原平皓(天津)生物技术有限公司 | 一种提取rna的方法和试剂盒 |
| EP2707510B1 (en) * | 2011-05-12 | 2017-07-12 | Exact Sciences Corporation | Isolation of nucleic acids |
| AU2012348904B2 (en) * | 2011-12-06 | 2018-01-18 | Qiagen Gmbh | Recovery of a biomolecule comprising aqueous phase from a multiphasic mixture |
| GB2561425B (en) * | 2012-03-16 | 2019-01-16 | Cambridge Entpr Ltd | Apparatus for obtaining liquid from a solid phase |
-
2015
- 2015-04-10 US US15/303,062 patent/US20170029810A1/en not_active Abandoned
- 2015-04-10 ES ES15719064T patent/ES2708205T3/es active Active
- 2015-04-10 EP EP15719064.6A patent/EP3129498B1/en not_active Not-in-force
- 2015-04-10 CN CN201580019186.8A patent/CN106164270A/zh active Pending
- 2015-04-10 WO PCT/US2015/025335 patent/WO2015157650A1/en not_active Ceased
- 2015-04-10 KR KR1020167031367A patent/KR20160138578A/ko not_active Withdrawn
- 2015-04-10 JP JP2017504620A patent/JP6562389B2/ja active Active
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