JP2017510305A5 - - Google Patents
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- Publication number
- JP2017510305A5 JP2017510305A5 JP2017504620A JP2017504620A JP2017510305A5 JP 2017510305 A5 JP2017510305 A5 JP 2017510305A5 JP 2017504620 A JP2017504620 A JP 2017504620A JP 2017504620 A JP2017504620 A JP 2017504620A JP 2017510305 A5 JP2017510305 A5 JP 2017510305A5
- Authority
- JP
- Japan
- Prior art keywords
- water
- filter
- alcohol
- immiscible fluid
- wash
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 9
- 239000012530 fluid Substances 0.000 claims 8
- 238000004140 cleaning Methods 0.000 claims 5
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 238000005406 washing Methods 0.000 claims 5
- 230000005484 gravity Effects 0.000 claims 4
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000003480 eluent Substances 0.000 claims 2
- 229920001477 hydrophilic polymer Polymers 0.000 claims 2
- 229920001410 Microfiber Polymers 0.000 claims 1
- 239000002202 Polyethylene glycol Substances 0.000 claims 1
- 239000011521 glass Substances 0.000 claims 1
- 239000003658 microfiber Substances 0.000 claims 1
- 229920001223 polyethylene glycol Polymers 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461978322P | 2014-04-11 | 2014-04-11 | |
| US61/978,322 | 2014-04-11 | ||
| PCT/US2015/025335 WO2015157650A1 (en) | 2014-04-11 | 2015-04-10 | Nucleic acid purification method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017510305A JP2017510305A (ja) | 2017-04-13 |
| JP2017510305A5 true JP2017510305A5 (enExample) | 2018-06-07 |
| JP6562389B2 JP6562389B2 (ja) | 2019-08-21 |
Family
ID=53008879
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017504620A Active JP6562389B2 (ja) | 2014-04-11 | 2015-04-10 | 核酸の精製方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20170029810A1 (enExample) |
| EP (1) | EP3129498B1 (enExample) |
| JP (1) | JP6562389B2 (enExample) |
| KR (1) | KR20160138578A (enExample) |
| CN (1) | CN106164270A (enExample) |
| ES (1) | ES2708205T3 (enExample) |
| WO (1) | WO2015157650A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10287570B2 (en) * | 2014-11-21 | 2019-05-14 | Occam Biolabs, Inc. | System and method for collecting a sample of nucleic acid |
| PL413551A1 (pl) * | 2015-08-18 | 2017-02-27 | Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością | Sposób i system do izolacji kwasów nukleinowych |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| WO2019066363A2 (ko) * | 2017-09-27 | 2019-04-04 | 고려대학교 산학협력단 | 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템 |
| KR101941787B1 (ko) * | 2017-11-13 | 2019-04-12 | 고려대학교 산학협력단 | 타겟 물질을 추출하기 위한 추출 장치 |
| US12383894B2 (en) | 2019-02-14 | 2025-08-12 | Korea University Research And Business Foundation | Extraction apparatus, extraction method, and fluidic chip for extracting target material |
| GB2581489B (en) * | 2019-02-15 | 2021-02-24 | Revolugen Ltd | Purification method |
| US20220298586A1 (en) | 2019-08-23 | 2022-09-22 | Fujifilm Wako Pure Chemical Corporation | Primer pair for detecting pneumocystis jirovecii, method for detecting pneumocystis jirovecii using same and reagent kit therefor |
| CN110724633A (zh) * | 2019-11-11 | 2020-01-24 | 浙江汇泽医药科技有限公司 | 一种微量细胞核酸提取与扩增系统及工艺 |
| WO2021124960A1 (ja) | 2019-12-18 | 2021-06-24 | 富士フイルム和光純薬株式会社 | マイコバクテリウム・ツベルクローシス、マイコバクテリウム・アビウム及びマイコバクテリウム・イントラセルラーを検出するためのプライマーセット及びこれを用いた方法、並びにそのための試薬キット |
| WO2022030605A1 (ja) * | 2020-08-07 | 2022-02-10 | 株式会社ゴーフォトン | 核酸抽出容器および核酸抽出方法 |
| JP7784545B2 (ja) * | 2022-06-14 | 2025-12-11 | 株式会社日立ハイテク | 核酸溶離液及び当該核酸溶離液を用いた核酸の溶離方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69433425T2 (de) * | 1993-08-30 | 2004-10-07 | Promega Corp | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
| JP4025399B2 (ja) * | 1997-10-28 | 2007-12-19 | 株式会社日立製作所 | 核酸の回収方法及び装置 |
| AU2001238616A1 (en) * | 2000-02-23 | 2001-09-03 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| US6706858B2 (en) * | 2001-05-11 | 2004-03-16 | Qiagen Gmbh | Use of alkanes for contamination-free purification or separation of biopolymers |
| CN102132156B (zh) * | 2008-08-01 | 2014-11-26 | 生物风险公司 | 用于物质的纯化、分离、脱盐或缓冲液/溶剂交换的装置和方法 |
| CA2769219A1 (en) * | 2009-08-04 | 2011-02-10 | E.I. Du Pont De Nemours And Company | Process and device for collecting nucleic acids of microorganisms from a particulate sample |
| JP6096660B2 (ja) * | 2010-09-02 | 2017-03-15 | キアゲン ゲーエムベーハー | 低分子標的核酸を含む標的核酸を高収量で単離するための方法 |
| CN101935648B (zh) * | 2010-09-13 | 2013-04-17 | 原平皓(天津)生物技术有限公司 | 一种提取rna的方法和试剂盒 |
| CN103649298A (zh) * | 2011-05-12 | 2014-03-19 | 精密科学公司 | 核酸的分离 |
| WO2013083260A1 (en) * | 2011-12-06 | 2013-06-13 | Qiagen Gmbh | Recovery of a biomolecule comprising aqueous phase from a multiphasic mixture |
| GB2501238B (en) * | 2012-03-16 | 2018-10-24 | Cambridge Entpr Ltd | Methods for obtaining liquid from a solid phase |
-
2015
- 2015-04-10 US US15/303,062 patent/US20170029810A1/en not_active Abandoned
- 2015-04-10 KR KR1020167031367A patent/KR20160138578A/ko not_active Withdrawn
- 2015-04-10 JP JP2017504620A patent/JP6562389B2/ja active Active
- 2015-04-10 EP EP15719064.6A patent/EP3129498B1/en not_active Not-in-force
- 2015-04-10 CN CN201580019186.8A patent/CN106164270A/zh active Pending
- 2015-04-10 WO PCT/US2015/025335 patent/WO2015157650A1/en not_active Ceased
- 2015-04-10 ES ES15719064T patent/ES2708205T3/es active Active
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