CN106164270A - 核酸纯化方法 - Google Patents

核酸纯化方法 Download PDF

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Publication number
CN106164270A
CN106164270A CN201580019186.8A CN201580019186A CN106164270A CN 106164270 A CN106164270 A CN 106164270A CN 201580019186 A CN201580019186 A CN 201580019186A CN 106164270 A CN106164270 A CN 106164270A
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CN
China
Prior art keywords
filter
solution
wash
immiscible fluid
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201580019186.8A
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English (en)
Chinese (zh)
Inventor
滨田貴俊
大卫·斯温森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fuji Film And Light Pure Drug Co Ltd
Original Assignee
Wako Pure Chemical Industries Ltd
Wako Life Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd, Wako Life Sciences Inc filed Critical Wako Pure Chemical Industries Ltd
Publication of CN106164270A publication Critical patent/CN106164270A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201580019186.8A 2014-04-11 2015-04-10 核酸纯化方法 Pending CN106164270A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461978322P 2014-04-11 2014-04-11
US61/978,322 2014-04-11
PCT/US2015/025335 WO2015157650A1 (en) 2014-04-11 2015-04-10 Nucleic acid purification method

Publications (1)

Publication Number Publication Date
CN106164270A true CN106164270A (zh) 2016-11-23

Family

ID=53008879

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580019186.8A Pending CN106164270A (zh) 2014-04-11 2015-04-10 核酸纯化方法

Country Status (7)

Country Link
US (1) US20170029810A1 (enExample)
EP (1) EP3129498B1 (enExample)
JP (1) JP6562389B2 (enExample)
KR (1) KR20160138578A (enExample)
CN (1) CN106164270A (enExample)
ES (1) ES2708205T3 (enExample)
WO (1) WO2015157650A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
CN114540343A (zh) * 2019-02-15 2022-05-27 雷沃卢金有限公司 纯化方法
CN115702238A (zh) * 2020-08-07 2023-02-14 光技光电集团日本分公司 核酸提取容器和核酸提取方法

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2729113C2 (ru) * 2014-11-21 2020-08-04 Оккам Байолэбс, Инк. Система и способ сбора образца нуклеиновой кислоты
PL413551A1 (pl) * 2015-08-18 2017-02-27 Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością Sposób i system do izolacji kwasów nukleinowych
DE102017206155A1 (de) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption von Nukleinsäuren
WO2019066363A2 (ko) * 2017-09-27 2019-04-04 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템
KR101941787B1 (ko) * 2017-11-13 2019-04-12 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치
US12383894B2 (en) 2019-02-14 2025-08-12 Korea University Research And Business Foundation Extraction apparatus, extraction method, and fluidic chip for extracting target material
WO2021039540A1 (ja) 2019-08-23 2021-03-04 富士フイルム和光純薬株式会社 ニューモシスチス・イロベチイ検出用プライマー対、これを用いたニューモシスチス・イロベチイの検出方法及びそのための試薬キット
EP4079851A4 (en) 2019-12-18 2023-06-07 FUJIFILM Corporation SET OF PRIMER FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS, MYCOBACTERIUM AVIUM AND MYCOBACTERIUM INTRACELLULARE AND METHODS OF USE THEREOF AND REAGENT KIT THEREFOR

Citations (5)

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Publication number Priority date Publication date Assignee Title
US20030100705A1 (en) * 2001-05-11 2003-05-29 Thomas Rothmann Use of alkanes for contamination-free purification or separation of biopolymers
US7067287B1 (en) * 1997-10-28 2006-06-27 Hitachi, Ltd. Method for recovery of nucleic acids
CN101935648A (zh) * 2010-09-13 2011-01-05 原平皓(天津)生物技术有限公司 一种提取rna的方法和试剂盒
WO2012028737A1 (en) * 2010-09-02 2012-03-08 Qiagen Gmbh Method for isolating a target nucleic acid including small target nucleic acids with high yield
US20130274454A1 (en) * 2012-03-16 2013-10-17 Cambridge Enterprise Limited Methods for obtaining liquid from a solid phase

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3696238B2 (ja) * 1993-08-30 2005-09-14 プロメガ・コーポレイシヨン 核酸精製用組成物及び方法
WO2001062976A1 (en) * 2000-02-23 2001-08-30 Wen Shao Rapid nucleic acid separation, isolation and purification methods
CN103657145A (zh) * 2008-08-01 2014-03-26 生物风险公司 用于物质的纯化、分离、脱盐或缓冲液/溶剂交换的装置和方法
EP2462226A1 (en) * 2009-08-04 2012-06-13 E. I. Du Pont De Nemours And Company Process and device for collecting nucleic acids of microorganisms from a particulate sample
WO2012155072A2 (en) * 2011-05-12 2012-11-15 Exact Sciences Corporation Isolation of nucleic acids
EP2788484B1 (en) * 2011-12-06 2017-11-01 Qiagen GmbH Recovery of a biomolecule comprising aqueous phase from a multiphasic mixture

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7067287B1 (en) * 1997-10-28 2006-06-27 Hitachi, Ltd. Method for recovery of nucleic acids
US20030100705A1 (en) * 2001-05-11 2003-05-29 Thomas Rothmann Use of alkanes for contamination-free purification or separation of biopolymers
WO2012028737A1 (en) * 2010-09-02 2012-03-08 Qiagen Gmbh Method for isolating a target nucleic acid including small target nucleic acids with high yield
CN101935648A (zh) * 2010-09-13 2011-01-05 原平皓(天津)生物技术有限公司 一种提取rna的方法和试剂盒
US20130274454A1 (en) * 2012-03-16 2013-10-17 Cambridge Enterprise Limited Methods for obtaining liquid from a solid phase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BERRY, SCOTT M ET AL.: "One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)", 《LAB ON A CHIP》 *
肖志良 等: "基于液滴技术的微流控芯片实验室及其应用", 《色谱》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114540343A (zh) * 2019-02-15 2022-05-27 雷沃卢金有限公司 纯化方法
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
CN115702238A (zh) * 2020-08-07 2023-02-14 光技光电集团日本分公司 核酸提取容器和核酸提取方法

Also Published As

Publication number Publication date
KR20160138578A (ko) 2016-12-05
EP3129498A1 (en) 2017-02-15
US20170029810A1 (en) 2017-02-02
WO2015157650A1 (en) 2015-10-15
JP2017510305A (ja) 2017-04-13
JP6562389B2 (ja) 2019-08-21
EP3129498B1 (en) 2018-12-12
ES2708205T3 (es) 2019-04-09

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Effective date of registration: 20180409

Address after: Osaka Japan

Applicant after: Wako-chem Industries Co., Ltd.

Address before: Osaka City, Osaka of Japan

Applicant before: Wako-chem Industries Co., Ltd.

Applicant before: And light life science Co., Ltd.

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TA01 Transfer of patent application right

Effective date of registration: 20180709

Address after: Osaka Japan

Applicant after: Fuji film and light pure drug Co., Ltd.

Address before: Osaka Japan

Applicant before: Wako-chem Industries Co., Ltd.

TA01 Transfer of patent application right
RJ01 Rejection of invention patent application after publication

Application publication date: 20161123

RJ01 Rejection of invention patent application after publication