KR20160138578A - 핵산 정제 방법 - Google Patents

핵산 정제 방법 Download PDF

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Publication number
KR20160138578A
KR20160138578A KR1020167031367A KR20167031367A KR20160138578A KR 20160138578 A KR20160138578 A KR 20160138578A KR 1020167031367 A KR1020167031367 A KR 1020167031367A KR 20167031367 A KR20167031367 A KR 20167031367A KR 20160138578 A KR20160138578 A KR 20160138578A
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KR
South Korea
Prior art keywords
solution
filter
alcohol
nucleic acid
fluid
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Withdrawn
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KR1020167031367A
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English (en)
Korean (ko)
Inventor
다카토시 하마다
데이비드 스웬슨
Original Assignee
와꼬 쥰야꾸 고교 가부시키가이샤
와코 라이프 사이언시스 인코포레이티드
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Application filed by 와꼬 쥰야꾸 고교 가부시키가이샤, 와코 라이프 사이언시스 인코포레이티드 filed Critical 와꼬 쥰야꾸 고교 가부시키가이샤
Publication of KR20160138578A publication Critical patent/KR20160138578A/ko
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020167031367A 2014-04-11 2015-04-10 핵산 정제 방법 Withdrawn KR20160138578A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461978322P 2014-04-11 2014-04-11
US61/978,322 2014-04-11
PCT/US2015/025335 WO2015157650A1 (en) 2014-04-11 2015-04-10 Nucleic acid purification method

Publications (1)

Publication Number Publication Date
KR20160138578A true KR20160138578A (ko) 2016-12-05

Family

ID=53008879

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020167031367A Withdrawn KR20160138578A (ko) 2014-04-11 2015-04-10 핵산 정제 방법

Country Status (7)

Country Link
US (1) US20170029810A1 (enExample)
EP (1) EP3129498B1 (enExample)
JP (1) JP6562389B2 (enExample)
KR (1) KR20160138578A (enExample)
CN (1) CN106164270A (enExample)
ES (1) ES2708205T3 (enExample)
WO (1) WO2015157650A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019093652A1 (ko) * 2017-11-13 2019-05-16 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치
WO2019066363A3 (ko) * 2017-09-27 2019-06-06 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템
US12383894B2 (en) 2019-02-14 2025-08-12 Korea University Research And Business Foundation Extraction apparatus, extraction method, and fluidic chip for extracting target material

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10287570B2 (en) * 2014-11-21 2019-05-14 Occam Biolabs, Inc. System and method for collecting a sample of nucleic acid
PL413551A1 (pl) * 2015-08-18 2017-02-27 Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością Sposób i system do izolacji kwasów nukleinowych
DE102017206155A1 (de) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption von Nukleinsäuren
GB2581489B (en) * 2019-02-15 2021-02-24 Revolugen Ltd Purification method
JP7357063B2 (ja) 2019-08-23 2023-10-05 富士フイルム株式会社 ニューモシスチス・イロベチイ検出用プライマー対、これを用いたニューモシスチス・イロベチイの検出方法及びそのための試薬キット
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
EP4079851A4 (en) 2019-12-18 2023-06-07 FUJIFILM Corporation SET OF PRIMER FOR THE DETECTION OF MYCOBACTERIUM TUBERCULOSIS, MYCOBACTERIUM AVIUM AND MYCOBACTERIUM INTRACELLULARE AND METHODS OF USE THEREOF AND REAGENT KIT THEREFOR
JPWO2022030605A1 (enExample) * 2020-08-07 2022-02-10

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU689815B2 (en) * 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
WO2001062976A1 (en) * 2000-02-23 2001-08-30 Wen Shao Rapid nucleic acid separation, isolation and purification methods
US6706858B2 (en) * 2001-05-11 2004-03-16 Qiagen Gmbh Use of alkanes for contamination-free purification or separation of biopolymers
CA2730312C (en) * 2008-08-01 2016-08-16 Bioventures, Inc. Devices and methods for the purification, isolation, desalting or buffer/solvent exchange of substances
JP2013500742A (ja) * 2009-08-04 2013-01-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 粒状物試料から微生物の核酸を回収するための方法およびデバイス
EP2611916B1 (en) * 2010-09-02 2016-12-28 Qiagen GmbH Method for isolating a target nucleic acid including small target nucleic acids with high yield
CN101935648B (zh) * 2010-09-13 2013-04-17 原平皓(天津)生物技术有限公司 一种提取rna的方法和试剂盒
CN103649298A (zh) * 2011-05-12 2014-03-19 精密科学公司 核酸的分离
JP6278568B2 (ja) * 2011-12-06 2018-02-14 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 多相混合物からの生体分子を含む水相の回収
GB2561425B (en) * 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019066363A3 (ko) * 2017-09-27 2019-06-06 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템
WO2019093652A1 (ko) * 2017-11-13 2019-05-16 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치
US12383894B2 (en) 2019-02-14 2025-08-12 Korea University Research And Business Foundation Extraction apparatus, extraction method, and fluidic chip for extracting target material

Also Published As

Publication number Publication date
EP3129498B1 (en) 2018-12-12
JP2017510305A (ja) 2017-04-13
WO2015157650A1 (en) 2015-10-15
JP6562389B2 (ja) 2019-08-21
CN106164270A (zh) 2016-11-23
EP3129498A1 (en) 2017-02-15
ES2708205T3 (es) 2019-04-09
US20170029810A1 (en) 2017-02-02

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PA0105 International application

Patent event date: 20161109

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
N231 Notification of change of applicant
PN2301 Change of applicant

Patent event date: 20180328

Comment text: Notification of Change of Applicant

Patent event code: PN23011R01D

PC1203 Withdrawal of no request for examination