JP6562389B2 - 核酸の精製方法 - Google Patents

核酸の精製方法 Download PDF

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Publication number
JP6562389B2
JP6562389B2 JP2017504620A JP2017504620A JP6562389B2 JP 6562389 B2 JP6562389 B2 JP 6562389B2 JP 2017504620 A JP2017504620 A JP 2017504620A JP 2017504620 A JP2017504620 A JP 2017504620A JP 6562389 B2 JP6562389 B2 JP 6562389B2
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filter
water
wash
solution
immiscible
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JP2017510305A (ja
JP2017510305A5 (enExample
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貴俊 浜田
貴俊 浜田
スウェンソン デイビッド
スウェンソン デイビッド
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Fujifilm Wako Pure Chemical Corp
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Fujifilm Wako Pure Chemical Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2017504620A 2014-04-11 2015-04-10 核酸の精製方法 Active JP6562389B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461978322P 2014-04-11 2014-04-11
US61/978,322 2014-04-11
PCT/US2015/025335 WO2015157650A1 (en) 2014-04-11 2015-04-10 Nucleic acid purification method

Publications (3)

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JP2017510305A JP2017510305A (ja) 2017-04-13
JP2017510305A5 JP2017510305A5 (enExample) 2018-06-07
JP6562389B2 true JP6562389B2 (ja) 2019-08-21

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JP2017504620A Active JP6562389B2 (ja) 2014-04-11 2015-04-10 核酸の精製方法

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US (1) US20170029810A1 (enExample)
EP (1) EP3129498B1 (enExample)
JP (1) JP6562389B2 (enExample)
KR (1) KR20160138578A (enExample)
CN (1) CN106164270A (enExample)
ES (1) ES2708205T3 (enExample)
WO (1) WO2015157650A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2729113C2 (ru) * 2014-11-21 2020-08-04 Оккам Байолэбс, Инк. Система и способ сбора образца нуклеиновой кислоты
PL413551A1 (pl) * 2015-08-18 2017-02-27 Curiosity Diagnostics Spółka Z Ograniczoną Odpowiedzialnością Sposób i system do izolacji kwasów nukleinowych
DE102017206155A1 (de) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption von Nukleinsäuren
WO2019066363A2 (ko) * 2017-09-27 2019-04-04 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템
KR101941787B1 (ko) * 2017-11-13 2019-04-12 고려대학교 산학협력단 타겟 물질을 추출하기 위한 추출 장치
EP3925704A4 (en) * 2019-02-14 2022-11-09 Korea University Research and Business Foundation EXTRACTION DEVICE, EXTRACTION METHOD AND FLUID CHIP FOR EXTRACTING TARGET MATERIAL
GB2581489B (en) * 2019-02-15 2021-02-24 Revolugen Ltd Purification method
WO2021039540A1 (ja) 2019-08-23 2021-03-04 富士フイルム和光純薬株式会社 ニューモシスチス・イロベチイ検出用プライマー対、これを用いたニューモシスチス・イロベチイの検出方法及びそのための試薬キット
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
KR20220098246A (ko) 2019-12-18 2022-07-11 후지필름 가부시키가이샤 마이코박테륨·투베르쿨로시스, 마이코박테륨·아비움 및 마이코박테륨·인트라셀룰라레를 검출하기 위한 프라이머 세트 및 이것을 이용한 방법, 및 그를 위한 시약 키트
WO2022030605A1 (ja) * 2020-08-07 2022-02-10 株式会社ゴーフォトン 核酸抽出容器および核酸抽出方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU689815B2 (en) * 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
JP4025399B2 (ja) * 1997-10-28 2007-12-19 株式会社日立製作所 核酸の回収方法及び装置
AU2001238616A1 (en) * 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
US6706858B2 (en) * 2001-05-11 2004-03-16 Qiagen Gmbh Use of alkanes for contamination-free purification or separation of biopolymers
EP3006934B1 (en) * 2008-08-01 2017-02-01 BioVentures, Inc., Device for the purification, isolation, desalting or buffer/solvent exchange of substances
JP2013500742A (ja) * 2009-08-04 2013-01-10 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 粒状物試料から微生物の核酸を回収するための方法およびデバイス
DK2611916T3 (en) * 2010-09-02 2017-04-10 Qiagen Gmbh PROCEDURE FOR INSULATING A TARGET NUCLEIC ACID, INCLUDING SMALL TARGET ACIDS, WITH HIGH Yield
CN101935648B (zh) * 2010-09-13 2013-04-17 原平皓(天津)生物技术有限公司 一种提取rna的方法和试剂盒
EP2707510B1 (en) * 2011-05-12 2017-07-12 Exact Sciences Corporation Isolation of nucleic acids
AU2012348904B2 (en) * 2011-12-06 2018-01-18 Qiagen Gmbh Recovery of a biomolecule comprising aqueous phase from a multiphasic mixture
GB2561425B (en) * 2012-03-16 2019-01-16 Cambridge Entpr Ltd Apparatus for obtaining liquid from a solid phase

Also Published As

Publication number Publication date
WO2015157650A1 (en) 2015-10-15
EP3129498B1 (en) 2018-12-12
ES2708205T3 (es) 2019-04-09
KR20160138578A (ko) 2016-12-05
US20170029810A1 (en) 2017-02-02
JP2017510305A (ja) 2017-04-13
CN106164270A (zh) 2016-11-23
EP3129498A1 (en) 2017-02-15

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