WO2019066363A3 - 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템 - Google Patents

타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템 Download PDF

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Publication number
WO2019066363A3
WO2019066363A3 PCT/KR2018/011026 KR2018011026W WO2019066363A3 WO 2019066363 A3 WO2019066363 A3 WO 2019066363A3 KR 2018011026 W KR2018011026 W KR 2018011026W WO 2019066363 A3 WO2019066363 A3 WO 2019066363A3
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Prior art keywords
extraction
porous membrane
target substance
elution buffer
sample kit
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PCT/KR2018/011026
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English (en)
French (fr)
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WO2019066363A2 (ko
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신세현
이호윤
나원휘
서창덕
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고려대학교 산학협력단
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Publication of WO2019066363A2 publication Critical patent/WO2019066363A2/ko
Publication of WO2019066363A3 publication Critical patent/WO2019066363A3/ko

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

본 발명은 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템에 관한 것이다. 본 발명에 따른 추출 장치는 시료 키트와, 상기 시료 키트의 내부에 설치되어 상기 타겟 물질이 바인딩되는 다공막과, 상기 시료 키트 내에서 상기 다공막에 적층되어 상기 다공막을 통과하면서 상기 다공막으로부터 상기 타겟 물질을 용출하는 용출 용액과, 상기 용출 용액과 상이한 극성을 가져 상기 용출 용액과 섞이지 않는 상태로 상기 시료 키트 내에서 상기 용출 용액에 적층되는 이극성 용액을 포함하며; 상기 용출 용액과 상기 이극성 용액이 순차적으로 상기 다공막을 통과하고; 상기 이극성 용액이 상기 다공막을 통과할 때 상기 다공막에 잔존하는 상기 용출 용액을 밀어내는 것을 특징으로 한다. 이에 따라, 원심 분리 방식을 이용하지 않거나 고속의 원심 분리를 적용하지 않으면서도 핵산과 같은 타겟 물질을 추출할 수 있다.
PCT/KR2018/011026 2017-09-27 2018-09-19 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템 WO2019066363A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20170124798 2017-09-27
KR10-2017-0124798 2017-09-27

Publications (2)

Publication Number Publication Date
WO2019066363A2 WO2019066363A2 (ko) 2019-04-04
WO2019066363A3 true WO2019066363A3 (ko) 2019-06-06

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Application Number Title Priority Date Filing Date
PCT/KR2018/011026 WO2019066363A2 (ko) 2017-09-27 2018-09-19 타겟 물질을 추출하기 위한 추출 장치, 추출 방법 및 추출 시스템

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102019206062A1 (de) * 2019-04-29 2020-10-29 Robert Bosch Gmbh Verfahren und Vorrichtung zur Isolierung von biologischen Bestandteilen aus einer Probe
CN111545256B (zh) * 2020-03-31 2022-05-13 深圳市刚竹医疗科技有限公司 离心微流控芯片

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100119231A (ko) * 2009-04-30 2010-11-09 주식회사 자이벡 추출 장치, 핵산 추출 장치 및 핵산 추출 방법
KR20140095342A (ko) * 2013-01-24 2014-08-01 삼성전자주식회사 핵산 분석용 미세 유체 시스템
KR20160045560A (ko) * 2014-10-17 2016-04-27 다니엘 라이 신속한 핵산 추출 방법 및 장치
KR20160138578A (ko) * 2014-04-11 2016-12-05 와꼬 쥰야꾸 고교 가부시키가이샤 핵산 정제 방법
KR20170012806A (ko) * 2015-07-24 2017-02-03 케이맥(주) 다이렉트 용리 반응에 의한 핵산 추출 및 증폭 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20100119231A (ko) * 2009-04-30 2010-11-09 주식회사 자이벡 추출 장치, 핵산 추출 장치 및 핵산 추출 방법
KR20140095342A (ko) * 2013-01-24 2014-08-01 삼성전자주식회사 핵산 분석용 미세 유체 시스템
KR20160138578A (ko) * 2014-04-11 2016-12-05 와꼬 쥰야꾸 고교 가부시키가이샤 핵산 정제 방법
KR20160045560A (ko) * 2014-10-17 2016-04-27 다니엘 라이 신속한 핵산 추출 방법 및 장치
KR20170012806A (ko) * 2015-07-24 2017-02-03 케이맥(주) 다이렉트 용리 반응에 의한 핵산 추출 및 증폭 방법

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