JP2016502862A5 - - Google Patents
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- JP2016502862A5 JP2016502862A5 JP2015551774A JP2015551774A JP2016502862A5 JP 2016502862 A5 JP2016502862 A5 JP 2016502862A5 JP 2015551774 A JP2015551774 A JP 2015551774A JP 2015551774 A JP2015551774 A JP 2015551774A JP 2016502862 A5 JP2016502862 A5 JP 2016502862A5
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- capture surface
- membranes
- column
- inner tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 20
- 239000012472 biological sample Substances 0.000 claims description 14
- 239000012160 loading buffer Substances 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 6
- 150000007523 nucleic acids Chemical class 0.000 claims 6
- 102000039446 nucleic acids Human genes 0.000 claims 6
- 239000011148 porous material Substances 0.000 claims 4
- 125000001453 quaternary ammonium group Chemical group 0.000 claims 4
- 239000004627 regenerated cellulose Substances 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000012228 culture supernatant Substances 0.000 claims 2
- 210000002381 plasma Anatomy 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 210000002700 urine Anatomy 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 239000012139 lysis buffer Substances 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361748575P | 2013-01-03 | 2013-01-03 | |
| US61/748,575 | 2013-01-03 | ||
| PCT/US2014/010173 WO2014107571A1 (en) | 2013-01-03 | 2014-01-03 | Methods for isolating microvesicles |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016502862A JP2016502862A (ja) | 2016-02-01 |
| JP2016502862A5 true JP2016502862A5 (enExample) | 2018-06-14 |
| JP6449781B2 JP6449781B2 (ja) | 2019-01-09 |
Family
ID=51062472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015551774A Active JP6449781B2 (ja) | 2013-01-03 | 2014-01-03 | 微細小胞の単離方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20150353920A1 (enExample) |
| EP (2) | EP3499212B1 (enExample) |
| JP (1) | JP6449781B2 (enExample) |
| CN (2) | CN110106229B (enExample) |
| AU (2) | AU2014203987B2 (enExample) |
| BR (1) | BR112015016136A2 (enExample) |
| CA (1) | CA2897207C (enExample) |
| IL (2) | IL239786B (enExample) |
| MX (2) | MX392552B (enExample) |
| WO (1) | WO2014107571A1 (enExample) |
Families Citing this family (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012155014A1 (en) * | 2011-05-11 | 2012-11-15 | Exosome Diagnostics, Inc. | Nucleic acid extraction from heterogeneous biological materials |
| TR201901310T4 (tr) | 2013-03-14 | 2019-02-21 | Translate Bio Inc | Mesajcı RNA'nın saflaştırılması yöntemleri. |
| US20170165334A1 (en) * | 2015-12-11 | 2017-06-15 | Tianxin Wang | Methods to Treat Diseases with Protein, Peptide, Antigen Modification and Hemopurification |
| US11268085B2 (en) | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| SG11201700146QA (en) | 2014-07-09 | 2017-02-27 | Exosome Diagnostics Inc | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| US20170211139A1 (en) * | 2014-07-11 | 2017-07-27 | Exosome Diagnostics, Inc. | Compositions and methods for detecting rare sequence variants in nucleic acid sequencing |
| WO2016065295A1 (en) | 2014-10-24 | 2016-04-28 | Dae Hyun Kim | Enrichment of small nucleic acids |
| EP3112474A1 (en) * | 2015-06-29 | 2017-01-04 | Evonik Degussa GmbH | Method of detecting avian necrotic enteritis |
| WO2017062901A2 (en) * | 2015-10-07 | 2017-04-13 | Tymora Analytical Operations Llc | Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis |
| SG11201809005TA (en) * | 2016-04-15 | 2018-11-29 | Exosome Diagnostics Inc | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| CN105716923A (zh) * | 2016-04-21 | 2016-06-29 | 华南理工大学 | 一种聚合物囊泡扫描电镜样品的制备方法 |
| EP3452613B1 (en) | 2016-05-05 | 2021-12-29 | Exosome Diagnostics, Inc. | Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection |
| AU2017263886B2 (en) * | 2016-05-13 | 2023-11-09 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
| EP3527980B1 (en) * | 2016-10-13 | 2022-03-02 | H.U. Group Research Institute G.K. | Method for recovering extracellular vesicles |
| EP3529374B1 (en) | 2016-10-21 | 2024-04-03 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| CN110446790B (zh) | 2016-11-30 | 2023-03-31 | 外来体诊断公司 | 使用外来体rna和无细胞dna检测血浆中的突变的方法和组合物 |
| JP7376091B2 (ja) * | 2016-12-23 | 2023-11-08 | エクソファーマ リミテッド | 微小胞およびエキソソームの精製または単離のための方法および組成物 |
| WO2018126278A2 (en) | 2017-01-02 | 2018-07-05 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| RU2769503C2 (ru) | 2017-05-12 | 2022-04-01 | Эвоник Оперейшенс ГмбХ | Способ выявления заболеваний, вызываемых c. perfringens, у животных |
| WO2018213392A1 (en) | 2017-05-17 | 2018-11-22 | Exosome Diagnostics, Inc. | Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection |
| CN107271655A (zh) * | 2017-05-18 | 2017-10-20 | 成都中医药大学附属医院 | 一种检测尿外泌体荷载miRNAs的试剂盒和方法 |
| CN111133106A (zh) * | 2017-07-12 | 2020-05-08 | 外来体诊断公司 | 用于分离和富集生物流体来源的细胞外囊泡的方法及其使用方法 |
| WO2019013991A2 (en) * | 2017-07-12 | 2019-01-17 | Illumina, Inc. | METHODS, SYSTEMS AND MATERIALS FOR EXTRACTING NUCLEIC ACIDS |
| EP3655531B1 (en) * | 2017-07-18 | 2024-09-04 | Exosome Diagnostics, Inc. | Sequencing of nucleic acids associated with exosomal isolation from patients with glioblastoma multiforme |
| JP2020528766A (ja) * | 2017-07-26 | 2020-10-01 | ロゼッタ エクソソーム | 陽イオンを利用した細胞外小胞体の分離方法 |
| US11260347B2 (en) * | 2017-12-26 | 2022-03-01 | Limited Liability Company “Prostagnost” | Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration |
| US20210011027A1 (en) | 2018-03-02 | 2021-01-14 | Evonik Operations Gmbh | In vitro method for detecting intestinal barrier failure in animals by determining ovotransferrin |
| US12378608B2 (en) | 2018-06-06 | 2025-08-05 | Exosome Diagnostics, Inc. | Methods for developing urine biomarkers and for detecting bladder cancer |
| CA3104349A1 (en) | 2018-06-18 | 2019-12-26 | Exopharm Limited | Methods and compositions for purification or isolation of microvesicles and exosomes |
| CN108841777A (zh) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | 基于静电吸附的胞外囊泡及其内含物的提取方法及装置 |
| CA3102658A1 (en) * | 2018-06-27 | 2020-01-02 | The Trustees Of Indiana University | Methods for analyzing dna in urine |
| US10955379B2 (en) | 2018-09-27 | 2021-03-23 | Taiwan Semiconductor Manufacturing Co., Ltd. | Differential sensing with BioFET sensors |
| CN109337859A (zh) * | 2018-10-08 | 2019-02-15 | 南昌大学 | 一种分离细胞表面囊泡的方法 |
| EP3875570A4 (en) * | 2018-10-30 | 2022-08-24 | Craif Inc. | DEVICE USED FOR CONTAINING EXTRACELLULAR VESICLES AND METHODS FOR PRESERVING AND TRANSPORTING EXTRACELLULAR VESICLES |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| JP2020092688A (ja) * | 2018-12-12 | 2020-06-18 | 国立大学法人東海国立大学機構 | マイクロｒｎａを含む体液抽出物 |
| CA3122457A1 (en) | 2018-12-14 | 2020-06-18 | Evonik Operations Gmbh | In vitro method for detecting avian intestinal dysbiosis |
| JP7344973B2 (ja) | 2019-03-01 | 2023-09-14 | マーシー バイオアナリティクス， インコーポレイテッド | 標的実体を検出するためのシステム、組成物、及び方法 |
| WO2020191369A1 (en) * | 2019-03-21 | 2020-09-24 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CN112048462B (zh) * | 2019-06-05 | 2022-08-23 | 北京丰特云基科技发展有限公司 | 一种基于阴离子多聚物修饰基质的细胞外囊泡分离富集方法 |
| WO2021055770A2 (en) | 2019-09-18 | 2021-03-25 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| US10984211B1 (en) | 2019-10-18 | 2021-04-20 | Taiwan Semiconductor Manufacturing Co., Ltd. | Semiconductor device with bioFET and biometric sensors |
| EP4077660A1 (en) * | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| EP4077659A1 (en) | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Enrichment method |
| JP7425403B2 (ja) * | 2020-02-17 | 2024-01-31 | 株式会社Ｊｖｃケンウッド | 生体試料分析方法 |
| CN113495029A (zh) * | 2020-04-01 | 2021-10-12 | 杨昆德 | 用于分离欲求物质的装置和方法 |
| CN115698265A (zh) * | 2020-04-24 | 2023-02-03 | 高丽大学校算学协力团 | 微囊泡分离方法及微囊泡分离装置 |
| CA3180572A1 (en) | 2020-05-29 | 2021-12-02 | Johan Skog | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| US20230366794A1 (en) * | 2020-09-23 | 2023-11-16 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CN112391382B (zh) * | 2020-12-07 | 2022-10-11 | 湖北盛齐安生物科技股份有限公司 | 一种快速提取囊泡dna的方法 |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| US20250163510A1 (en) | 2022-02-18 | 2025-05-22 | Exosome Diagnostics, Inc. | Use of microvesicle signatures in the identification and treatment of renal disorders |
| EP4584397A1 (en) | 2022-09-07 | 2025-07-16 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2025057787A1 (ja) * | 2023-09-14 | 2025-03-20 | 東洋紡株式会社 | 細胞外小胞の製造方法 |
| CN117487739B (zh) * | 2023-10-30 | 2024-10-01 | 深圳市茵冠生物科技有限公司 | 一种大规模纯化293细胞外泌体的方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| JP2004105009A (ja) * | 2002-09-13 | 2004-04-08 | Dojindo Laboratories | テトラフェニルホウ素化合物から成る核酸分離用試薬とそれを用いる核酸分離方法 |
| US20110053157A1 (en) | 2008-02-01 | 2011-03-03 | The General Hospital Corporation | Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions |
| WO2010065765A2 (en) * | 2008-12-04 | 2010-06-10 | Aethlon Medical, Inc. | Affinity capture of circulating biomarkers |
| WO2010141862A2 (en) * | 2009-06-05 | 2010-12-09 | Mayo Foundation For Medical Education And Research | Methods and materials for isolating exosomes |
| US20120177574A1 (en) * | 2009-07-01 | 2012-07-12 | Aeon Medix Inc. | Microvesicles derived from nucleated, mammalian cells and use thereof |
| ES2624284T3 (es) * | 2010-07-07 | 2017-07-13 | Aethlon Medical Inc | Métodos para cuantificar exosomas |
| US20130295574A1 (en) * | 2010-11-10 | 2013-11-07 | Exosome Diagnostics, Inc. | Method for Isolation of Nucleic Acid Containing Particles and Extraction of Nucleic Acids Therefrom |
| SG190450A1 (en) * | 2010-12-20 | 2013-06-28 | Agency Science Tech & Res | Method of purifying exosomes |
| BR112013032232A2 (pt) * | 2011-06-16 | 2016-09-20 | Caris Life Sciences Switzerland Holdings S A R L | método de caracterização de câncer através do uso de biomarcador de ácido nucleico |
-
2014
- 2014-01-03 EP EP18199291.8A patent/EP3499212B1/en active Active
- 2014-01-03 BR BR112015016136A patent/BR112015016136A2/pt not_active IP Right Cessation
- 2014-01-03 AU AU2014203987A patent/AU2014203987B2/en active Active
- 2014-01-03 EP EP14735397.3A patent/EP2941629B1/en active Active
- 2014-01-03 WO PCT/US2014/010173 patent/WO2014107571A1/en not_active Ceased
- 2014-01-03 MX MX2019007742A patent/MX392552B/es unknown
- 2014-01-03 MX MX2015008727A patent/MX366153B/es active IP Right Grant
- 2014-01-03 US US14/759,238 patent/US20150353920A1/en not_active Abandoned
- 2014-01-03 JP JP2015551774A patent/JP6449781B2/ja active Active
- 2014-01-03 CN CN201910000819.0A patent/CN110106229B/zh active Active
- 2014-01-03 CN CN201480002901.2A patent/CN105026911B/zh active Active
- 2014-01-03 CA CA2897207A patent/CA2897207C/en active Active
-
2015
- 2015-07-05 IL IL239786A patent/IL239786B/en active IP Right Grant
-
2018
- 2018-04-20 AU AU2018202789A patent/AU2018202789B2/en active Active
-
2019
- 2019-06-19 IL IL267526A patent/IL267526B/en active IP Right Grant
-
2020
- 2020-06-05 US US16/893,966 patent/US20210171934A1/en not_active Abandoned
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