JP2016502862A5 - - Google Patents
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- JP2016502862A5 JP2016502862A5 JP2015551774A JP2015551774A JP2016502862A5 JP 2016502862 A5 JP2016502862 A5 JP 2016502862A5 JP 2015551774 A JP2015551774 A JP 2015551774A JP 2015551774 A JP2015551774 A JP 2015551774A JP 2016502862 A5 JP2016502862 A5 JP 2016502862A5
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- capture surface
- membranes
- column
- inner tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000000034 method Methods 0.000 claims description 20
- 239000012472 biological sample Substances 0.000 claims description 14
- 239000012160 loading buffer Substances 0.000 claims description 6
- 230000007935 neutral effect Effects 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 6
- 150000007523 nucleic acids Chemical class 0.000 claims 6
- 102000039446 nucleic acids Human genes 0.000 claims 6
- 239000011148 porous material Substances 0.000 claims 4
- 125000001453 quaternary ammonium group Chemical group 0.000 claims 4
- 239000004627 regenerated cellulose Substances 0.000 claims 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000012228 culture supernatant Substances 0.000 claims 2
- 210000002381 plasma Anatomy 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 210000002700 urine Anatomy 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 239000012139 lysis buffer Substances 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 108091070501 miRNA Proteins 0.000 claims 1
- 239000002679 microRNA Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361748575P | 2013-01-03 | 2013-01-03 | |
| US61/748,575 | 2013-01-03 | ||
| PCT/US2014/010173 WO2014107571A1 (en) | 2013-01-03 | 2014-01-03 | Methods for isolating microvesicles |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016502862A JP2016502862A (ja) | 2016-02-01 |
| JP2016502862A5 true JP2016502862A5 (enExample) | 2018-06-14 |
| JP6449781B2 JP6449781B2 (ja) | 2019-01-09 |
Family
ID=51062472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015551774A Active JP6449781B2 (ja) | 2013-01-03 | 2014-01-03 | 微細小胞の単離方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20150353920A1 (enExample) |
| EP (2) | EP3499212B1 (enExample) |
| JP (1) | JP6449781B2 (enExample) |
| CN (2) | CN110106229B (enExample) |
| AU (2) | AU2014203987B2 (enExample) |
| BR (1) | BR112015016136A2 (enExample) |
| CA (1) | CA2897207C (enExample) |
| IL (2) | IL239786B (enExample) |
| MX (2) | MX392552B (enExample) |
| WO (1) | WO2014107571A1 (enExample) |
Families Citing this family (57)
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| EP2707381B1 (en) * | 2011-05-11 | 2019-08-14 | Exosome Diagnostics, Inc. | Nucleic acid extraction from heterogeneous biological materials |
| KR20150128687A (ko) * | 2013-03-14 | 2015-11-18 | 샤이어 휴먼 지네틱 테라피즈 인크. | 메신저 rna의 정제 방법 |
| US20170165334A1 (en) * | 2015-12-11 | 2017-06-15 | Tianxin Wang | Methods to Treat Diseases with Protein, Peptide, Antigen Modification and Hemopurification |
| US11268085B2 (en) | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CA2954576C (en) | 2014-07-09 | 2020-08-11 | Daniel ENDERLE | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| US20170211139A1 (en) * | 2014-07-11 | 2017-07-27 | Exosome Diagnostics, Inc. | Compositions and methods for detecting rare sequence variants in nucleic acid sequencing |
| US9783799B2 (en) | 2014-10-24 | 2017-10-10 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| EP3112474A1 (en) | 2015-06-29 | 2017-01-04 | Evonik Degussa GmbH | Method of detecting avian necrotic enteritis |
| WO2017062901A2 (en) * | 2015-10-07 | 2017-04-13 | Tymora Analytical Operations Llc | Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis |
| US20190093172A1 (en) | 2016-04-15 | 2019-03-28 | Exosome Diagnostics, Inc. | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| CN105716923A (zh) * | 2016-04-21 | 2016-06-29 | 华南理工大学 | 一种聚合物囊泡扫描电镜样品的制备方法 |
| ES2908932T3 (es) | 2016-05-05 | 2022-05-04 | Exosome Diagnostics Inc | Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal |
| JP7037504B2 (ja) * | 2016-05-13 | 2022-03-16 | エクソサム ダイアグノスティクス，インコーポレイティド | 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法 |
| WO2018070479A1 (ja) | 2016-10-13 | 2018-04-19 | 合同会社みらか中央研究所 | 細胞外小胞の回収方法 |
| US11396676B2 (en) | 2016-10-21 | 2022-07-26 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| CA3044056A1 (en) | 2016-11-30 | 2018-06-07 | Exosome Diagnostics, Inc. | Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients |
| AU2017381410A1 (en) * | 2016-12-23 | 2019-08-08 | Exopharm Limited | Methods and compositions for purification or isolation of microvesicles and exosomes |
| WO2018126278A2 (en) | 2017-01-02 | 2018-07-05 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| EP3622082B1 (en) | 2017-05-12 | 2023-03-29 | Evonik Operations GmbH | Method for detecting c. perfringens induced disases in an avian flock |
| JP7225121B2 (ja) | 2017-05-17 | 2023-02-20 | エクソサム ダイアグノスティクス，インコーポレイティド | 微小胞核酸および／またはタンパク質、並びに腎移植拒絶反応マーカーとしてのその使用 |
| CN107271655A (zh) * | 2017-05-18 | 2017-10-20 | 成都中医药大学附属医院 | 一种检测尿外泌体荷载miRNAs的试剂盒和方法 |
| CN111133106A (zh) * | 2017-07-12 | 2020-05-08 | 外来体诊断公司 | 用于分离和富集生物流体来源的细胞外囊泡的方法及其使用方法 |
| US11390864B2 (en) * | 2017-07-12 | 2022-07-19 | Illumina, Inc. | Nucleic acid extraction materials, systems, and methods |
| WO2019018537A1 (en) * | 2017-07-18 | 2019-01-24 | Exosome Diagnostics, Inc. | NUCLEIC ACID SEQUENCING ASSOCIATED WITH EXOSOMAL ISOLATION IN PATIENTS WITH MULTIPLE GLIOBLASTOMA |
| CN111148828A (zh) * | 2017-07-26 | 2020-05-12 | 罗塞塔外排体株式会社 | 利用阳离子分离细胞外囊泡的方法 |
| US11260347B2 (en) * | 2017-12-26 | 2022-03-01 | Limited Liability Company “Prostagnost” | Method and device for separating extracellular vesicles from biological liquids with the aid of cascade ultrafiltration |
| KR20200128413A (ko) | 2018-03-02 | 2020-11-12 | 에보니크 오퍼레이션즈 게엠베하 | 오보트랜스페린을 결정함으로써 동물에서의 장 장벽 기능상실을 검출하기 위한 시험관내 방법 |
| CN112739832B (zh) | 2018-06-06 | 2024-06-04 | 外来体诊断公司 | 用于开发尿液生物标志物和用于检测膀胱癌的方法 |
| MX2020014024A (es) | 2018-06-18 | 2021-03-09 | Exopharm Ltd | Metodos y composiciones para la purificacion o aislamiento de microvesiculas y exosomas. |
| CN108841777A (zh) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | 基于静电吸附的胞外囊泡及其内含物的提取方法及装置 |
| WO2020006197A1 (en) * | 2018-06-27 | 2020-01-02 | The Trustees Of Indiana University | Methods for analyzing dna in urine |
| US10955379B2 (en) | 2018-09-27 | 2021-03-23 | Taiwan Semiconductor Manufacturing Co., Ltd. | Differential sensing with BioFET sensors |
| CN109337859A (zh) * | 2018-10-08 | 2019-02-15 | 南昌大学 | 一种分离细胞表面囊泡的方法 |
| JP7426725B2 (ja) * | 2018-10-30 | 2024-02-02 | Ｃｒａｉｆ株式会社 | 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法 |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| JP2020092688A (ja) * | 2018-12-12 | 2020-06-18 | 国立大学法人東海国立大学機構 | マイクロｒｎａを含む体液抽出物 |
| MX2021006917A (es) | 2018-12-14 | 2021-07-07 | Evonik Operations Gmbh | Procedimiento in vitro para la deteccion de disbiosis intestinal aviar. |
| AU2020233286B2 (en) | 2019-03-01 | 2022-10-20 | Mercy Bioanalytics, Inc. | Systems, compositions, and methods for target entity detection |
| US12233356B2 (en) | 2019-03-21 | 2025-02-25 | Lonza Sales Ag | Process for preparing extracellular vesicles |
| CN112048462B (zh) * | 2019-06-05 | 2022-08-23 | 北京丰特云基科技发展有限公司 | 一种基于阴离子多聚物修饰基质的细胞外囊泡分离富集方法 |
| EP4031865A2 (en) | 2019-09-18 | 2022-07-27 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| US10984211B1 (en) | 2019-10-18 | 2021-04-20 | Taiwan Semiconductor Manufacturing Co., Ltd. | Semiconductor device with bioFET and biometric sensors |
| US20230028205A1 (en) | 2019-12-16 | 2023-01-26 | Qiagen Gmbh | Enrichment method |
| EP4077660A1 (en) * | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Method for enriching vesicular rna |
| JP7425403B2 (ja) * | 2020-02-17 | 2024-01-31 | 株式会社Ｊｖｃケンウッド | 生体試料分析方法 |
| CN113495029A (zh) * | 2020-04-01 | 2021-10-12 | 杨昆德 | 用于分离欲求物质的装置和方法 |
| US20230168162A1 (en) * | 2020-04-24 | 2023-06-01 | Korea University Research And Business Foundation | Microvesicle isolation method and microvesicle isolation |
| WO2021243206A1 (en) | 2020-05-29 | 2021-12-02 | Exosome Diagnostics, Inc. | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| EP4217087A2 (en) * | 2020-09-23 | 2023-08-02 | Codiak BioSciences, Inc. | Process for preparing extracellular vesicles |
| CN112391382B (zh) * | 2020-12-07 | 2022-10-11 | 湖北盛齐安生物科技股份有限公司 | 一种快速提取囊泡dna的方法 |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| US20250075258A1 (en) | 2021-07-16 | 2025-03-06 | Exosome Diagnostics, Inc. | Methods of detecting sjogren's syndrome using salivary exosomes |
| EP4479561A1 (en) | 2022-02-18 | 2024-12-25 | Exosome Diagnostics, Inc. | Use of microvesicle signatures in the identification and treatment of renal disorders |
| US20260078448A1 (en) | 2022-09-07 | 2026-03-19 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2025057787A1 (ja) * | 2023-09-14 | 2025-03-20 | 東洋紡株式会社 | 細胞外小胞の製造方法 |
| CN117487739B (zh) * | 2023-10-30 | 2024-10-01 | 深圳市茵冠生物科技有限公司 | 一种大规模纯化293细胞外泌体的方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| JP2004105009A (ja) * | 2002-09-13 | 2004-04-08 | Dojindo Laboratories | テトラフェニルホウ素化合物から成る核酸分離用試薬とそれを用いる核酸分離方法 |
| CN105734128B (zh) | 2008-02-01 | 2021-05-18 | 总医院有限公司 | 微泡在医学疾病和病况的诊断、预后以及治疗中的用途 |
| WO2010065765A2 (en) * | 2008-12-04 | 2010-06-10 | Aethlon Medical, Inc. | Affinity capture of circulating biomarkers |
| WO2010141862A2 (en) * | 2009-06-05 | 2010-12-09 | Mayo Foundation For Medical Education And Research | Methods and materials for isolating exosomes |
| WO2011002239A2 (ko) * | 2009-07-01 | 2011-01-06 | 주식회사이언메딕스 | 포유류의 유핵세포에서 유래된 마이크로베시클 및 이의 용도 |
| WO2012006476A2 (en) * | 2010-07-07 | 2012-01-12 | Aethlon Medical, Inc. | Methods and compositions for quantifying exosomes |
| US20130295574A1 (en) * | 2010-11-10 | 2013-11-07 | Exosome Diagnostics, Inc. | Method for Isolation of Nucleic Acid Containing Particles and Extraction of Nucleic Acids Therefrom |
| WO2012087241A1 (en) * | 2010-12-20 | 2012-06-28 | Agency For Science, Technology And Research | Method of purifying exosomes |
| WO2012174282A2 (en) * | 2011-06-16 | 2012-12-20 | Caris Life Sciences Luxembourg Holdings, S.A.R.L. | Biomarker compositions and methods |
-
2014
- 2014-01-03 CA CA2897207A patent/CA2897207C/en active Active
- 2014-01-03 CN CN201910000819.0A patent/CN110106229B/zh active Active
- 2014-01-03 AU AU2014203987A patent/AU2014203987B2/en active Active
- 2014-01-03 EP EP18199291.8A patent/EP3499212B1/en active Active
- 2014-01-03 JP JP2015551774A patent/JP6449781B2/ja active Active
- 2014-01-03 WO PCT/US2014/010173 patent/WO2014107571A1/en not_active Ceased
- 2014-01-03 CN CN201480002901.2A patent/CN105026911B/zh active Active
- 2014-01-03 MX MX2019007742A patent/MX392552B/es unknown
- 2014-01-03 US US14/759,238 patent/US20150353920A1/en not_active Abandoned
- 2014-01-03 MX MX2015008727A patent/MX366153B/es active IP Right Grant
- 2014-01-03 EP EP14735397.3A patent/EP2941629B1/en active Active
- 2014-01-03 BR BR112015016136A patent/BR112015016136A2/pt not_active IP Right Cessation
-
2015
- 2015-07-05 IL IL239786A patent/IL239786B/en active IP Right Grant
-
2018
- 2018-04-20 AU AU2018202789A patent/AU2018202789B2/en active Active
-
2019
- 2019-06-19 IL IL267526A patent/IL267526B/en active IP Right Grant
-
2020
- 2020-06-05 US US16/893,966 patent/US20210171934A1/en not_active Abandoned
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