JP6449781B2 - 微細小胞の単離方法 - Google Patents

微細小胞の単離方法 Download PDF

Info

Publication number
JP6449781B2
JP6449781B2 JP2015551774A JP2015551774A JP6449781B2 JP 6449781 B2 JP6449781 B2 JP 6449781B2 JP 2015551774 A JP2015551774 A JP 2015551774A JP 2015551774 A JP2015551774 A JP 2015551774A JP 6449781 B2 JP6449781 B2 JP 6449781B2
Authority
JP
Japan
Prior art keywords
rna
microvesicles
plasma
filter
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2015551774A
Other languages
English (en)
Japanese (ja)
Other versions
JP2016502862A5 (enExample
JP2016502862A (ja
Inventor
エンダール ダニエル
エンダール ダニエル
ラマチャンドラン アパルナ
ラマチャンドラン アパルナ
ヤン ハオホゥオン
ヤン ハオホゥオン
バーグホフ エミリー
バーグホフ エミリー
ウェイ タイ−フェン
ウェイ タイ−フェン
ナアホルム ミゲル
ナアホルム ミゲル
カール オロフ スコグ ヨハン
カール オロフ スコグ ヨハン
Original Assignee
エクソサム ダイアグノスティクス，インコーポレイティド
エクソサム ダイアグノスティクス，インコーポレイティド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by エクソサム ダイアグノスティクス，インコーポレイティド, エクソサム ダイアグノスティクス，インコーポレイティド filed Critical エクソサム ダイアグノスティクス，インコーポレイティド
Publication of JP2016502862A publication Critical patent/JP2016502862A/ja
Publication of JP2016502862A5 publication Critical patent/JP2016502862A5/ja
Application granted granted Critical
Publication of JP6449781B2 publication Critical patent/JP6449781B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015551774A 2013-01-03 2014-01-03 微細小胞の単離方法 Active JP6449781B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361748575P 2013-01-03 2013-01-03
US61/748,575 2013-01-03
PCT/US2014/010173 WO2014107571A1 (en) 2013-01-03 2014-01-03 Methods for isolating microvesicles

Publications (3)

Publication Number Publication Date
JP2016502862A JP2016502862A (ja) 2016-02-01
JP2016502862A5 JP2016502862A5 (enExample) 2018-06-14
JP6449781B2 true JP6449781B2 (ja) 2019-01-09

Family

ID=51062472

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015551774A Active JP6449781B2 (ja) 2013-01-03 2014-01-03 微細小胞の単離方法

Country Status (10)

Country Link
US (2) US20150353920A1 (enExample)
EP (2) EP2941629B1 (enExample)
JP (1) JP6449781B2 (enExample)
CN (2) CN105026911B (enExample)
AU (2) AU2014203987B2 (enExample)
BR (1) BR112015016136A2 (enExample)
CA (1) CA2897207C (enExample)
IL (2) IL239786B (enExample)
MX (2) MX366153B (enExample)
WO (1) WO2014107571A1 (enExample)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2835641C (en) * 2011-05-11 2019-05-28 Exosome Diagnostics, Inc. Nucleic acid extraction from heterogeneous biological materials
EA201591229A1 (ru) 2013-03-14 2016-01-29 Шир Хьюман Дженетик Терапис, Инк. Способы очистки матричной рнк
US20170165334A1 (en) * 2015-12-11 2017-06-15 Tianxin Wang Methods to Treat Diseases with Protein, Peptide, Antigen Modification and Hemopurification
US11268085B2 (en) 2014-05-27 2022-03-08 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
SG11201700146QA (en) 2014-07-09 2017-02-27 Exosome Diagnostics Inc Methods for isolating microvesicles and extracting nucleic acids from biological samples
WO2016007951A1 (en) * 2014-07-11 2016-01-14 KARNAKIS, Jennifer A. Compositions and methods for detecting rare sequence variants in nucleic acid sequencing
CN107405540A (zh) 2014-10-24 2017-11-28 雅培分子公司 小核酸的富集
EP3112474A1 (en) * 2015-06-29 2017-01-04 Evonik Degussa GmbH Method of detecting avian necrotic enteritis
WO2017062901A2 (en) * 2015-10-07 2017-04-13 Tymora Analytical Operations Llc Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis
JP2019513391A (ja) * 2016-04-15 2019-05-30 エクソサム ダイアグノスティクス，インコーポレイティド 未分化リンパ腫キナーゼ（ａｌｋ）核酸およびａｌｋ融合転写産物の血漿ベースの検出、並びに癌の診断と治療におけるそれらの使用
CN105716923A (zh) * 2016-04-21 2016-06-29 华南理工大学 一种聚合物囊泡扫描电镜样品的制备方法
ES2908932T3 (es) 2016-05-05 2022-05-04 Exosome Diagnostics Inc Perfilado de ácidos nucleicos de microvesícula y usos del mismo como características distintivas en diagnóstico de rechazo de trasplante renal
JP7037504B2 (ja) * 2016-05-13 2022-03-16 エクソサム ダイアグノスティクス，インコーポレイティド 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法
CN109891236A (zh) * 2016-10-13 2019-06-14 合同会社明乐康中央研究所 回收胞外囊泡的方法
US11396676B2 (en) 2016-10-21 2022-07-26 Exosome Diagnostics, Inc. Sequencing and analysis of exosome associated nucleic acids
WO2018102162A1 (en) 2016-11-30 2018-06-07 Exosome Diagnostics, Inc. Methods and compositions to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients
KR20190100174A (ko) * 2016-12-23 2019-08-28 엑소팜 리미티드 미세소포 및 엑소좀의 정제 또는 단리를 위한 방법 및 조성물
US20200149036A1 (en) 2017-01-02 2020-05-14 Exosome Diagnostics, Inc. Methods to distinguish rna and dna in a combined preparation
KR101875594B1 (ko) * 2017-01-13 2018-07-06 ㈜로제타엑소좀 금속을 이용한 세포밖 소포체의 분리 방법
ES2944359T3 (es) 2017-05-12 2023-06-20 Evonik Operations Gmbh Procedimiento de detección de enfermedades inducidas por C. perfringens en una bandada de aves
WO2018213392A1 (en) 2017-05-17 2018-11-22 Exosome Diagnostics, Inc. Microvesicle nucleic acids and/or proteins and uses thereof as markers of renal transplant rejection
CN107271655A (zh) * 2017-05-18 2017-10-20 成都中医药大学附属医院 一种检测尿外泌体荷载miRNAs的试剂盒和方法
EP3652315A4 (en) * 2017-07-12 2021-09-01 Exosome Diagnostics, Inc. METHODS FOR ISOLATION AND ENRICHMENT OF EXTRACELLULAR VESICLE POPULATIONS DERIVED FROM BIOFLUIDS, AND ASSOCIATED METHODS OF USE
EP3652314B1 (en) * 2017-07-12 2023-08-23 Illumina, Inc. Nucleic acid extraction materials and methods
US11345957B2 (en) 2017-07-18 2022-05-31 Exosome Diagnostics, Inc. Methods of treating glioblastoma in a subject informed by exosomal RNA signatures
JP2020528766A (ja) * 2017-07-26 2020-10-01 ロゼッタ エクソソーム 陽イオンを利用した細胞外小胞体の分離方法
WO2019132688A1 (ru) * 2017-12-26 2019-07-04 Общество С Ограниченной Ответственностью "Простагност" Способ и устройство для выделения внеклеточных везикул из биологических жидкостей с помощью каскадной ультрафильтрации
KR20200128413A (ko) 2018-03-02 2020-11-12 에보니크 오퍼레이션즈 게엠베하 오보트랜스페린을 결정함으로써 동물에서의 장 장벽 기능상실을 검출하기 위한 시험관내 방법
WO2019236853A1 (en) 2018-06-06 2019-12-12 Exosome Diagnostics, Inc. Methods for developing urine biomarkers and for detecting bladder cancer
CN112585446A (zh) 2018-06-18 2021-03-30 埃克波菲尔有限公司 用于纯化或分离微泡和外泌体的方法与组合物
CN108841777A (zh) * 2018-06-22 2018-11-20 北京恩泽康泰生物科技有限公司 基于静电吸附的胞外囊泡及其内含物的提取方法及装置
US20210239685A1 (en) * 2018-06-27 2021-08-05 The Trustees Of Indiana University Methods of analyzing dna in urine
US10955379B2 (en) 2018-09-27 2021-03-23 Taiwan Semiconductor Manufacturing Co., Ltd. Differential sensing with BioFET sensors
CN109337859A (zh) * 2018-10-08 2019-02-15 南昌大学 一种分离细胞表面囊泡的方法
JP7426725B2 (ja) * 2018-10-30 2024-02-02 Ｃｒａｉｆ株式会社 細胞外小胞を捕捉するために用いられるデバイス、細胞外小胞の保存方法および移送方法
EP3884045B1 (en) 2018-11-20 2023-01-11 Exosome Diagnostics, Inc. Methods for internal controls of microvesicle isolations
JP2020092688A (ja) * 2018-12-12 2020-06-18 国立大学法人東海国立大学機構 マイクロｒｎａを含む体液抽出物
BR112021011158A2 (pt) 2018-12-14 2021-08-31 Evonik Operations Gmbh Método in vitro para detectar disbiose intestinal aviária, uso do dito método e uso de isoleucil-arginina ou isômeros desta
CA3129868A1 (en) 2019-03-01 2020-09-10 Mercy Bioanalytics, Inc. Systems, compositions, and methods for target entity detection
EP3941607A1 (en) 2019-03-21 2022-01-26 Codiak BioSciences, Inc. Process for preparing extracellular vesicles
CN112048462B (zh) * 2019-06-05 2022-08-23 北京丰特云基科技发展有限公司 一种基于阴离子多聚物修饰基质的细胞外囊泡分离富集方法
US12493029B2 (en) 2019-09-18 2025-12-09 Exosome Diagnostics, Inc. Compositions, methods, and kits for the isolation of extracellular vesicles
US10984211B1 (en) 2019-10-18 2021-04-20 Taiwan Semiconductor Manufacturing Co., Ltd. Semiconductor device with bioFET and biometric sensors
WO2021122846A1 (en) 2019-12-16 2021-06-24 Qiagen Gmbh Enrichment method
CN114761556A (zh) * 2019-12-16 2022-07-15 凯杰有限公司 富集囊泡rna的方法
JP7425403B2 (ja) * 2020-02-17 2024-01-31 株式会社Ｊｖｃケンウッド 生体試料分析方法
CN113495029A (zh) * 2020-04-01 2021-10-12 杨昆德 用于分离欲求物质的装置和方法
CN115698265A (zh) * 2020-04-24 2023-02-03 高丽大学校算学协力团 微囊泡分离方法及微囊泡分离装置
AU2021281287A1 (en) 2020-05-29 2023-01-19 Bio-Techne Corporation Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection
US20230366794A1 (en) * 2020-09-23 2023-11-16 Codiak Biosciences, Inc. Process for preparing extracellular vesicles
CN112391382B (zh) * 2020-12-07 2022-10-11 湖北盛齐安生物科技股份有限公司 一种快速提取囊泡dna的方法
JP7773765B2 (ja) * 2020-12-18 2025-11-20 国立研究開発法人産業技術総合研究所 回収用キットおよび核酸含有溶液の調製方法
US20250075258A1 (en) 2021-07-16 2025-03-06 Exosome Diagnostics, Inc. Methods of detecting sjogren's syndrome using salivary exosomes
WO2023158869A1 (en) 2022-02-18 2023-08-24 Exosome Diagnostics, Inc. Use of microvesicle signatures in the identification and treatment of renal disorders
WO2024054572A1 (en) 2022-09-07 2024-03-14 Exosome Diagnostics, Inc. Methods of detecting sjögren's syndrome using salivary exosomes
KR20260070113A (ko) * 2023-09-14 2026-05-18 도요보 가부시키가이샤 세포 외 소포의 제조 방법
CN117487739B (zh) * 2023-10-30 2024-10-01 深圳市茵冠生物科技有限公司 一种大规模纯化293细胞外泌体的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935342A (en) * 1986-12-01 1990-06-19 Syngene, Inc. Method of isolating and purifying nucleic acids from biological samples
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5639606A (en) 1993-04-06 1997-06-17 The University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
FR2788780B1 (fr) 1999-01-27 2001-03-30 Ap Cells Inc Procede de preparation de vesicules membranaires
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US6812023B1 (en) 2000-04-27 2004-11-02 Anosys, Inc. Methods of producing membrane vesicles
JP2004105009A (ja) * 2002-09-13 2004-04-08 Dojindo Laboratories テトラフェニルホウ素化合物から成る核酸分離用試薬とそれを用いる核酸分離方法
AU2009212543B2 (en) 2008-02-01 2015-07-09 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2010065765A2 (en) * 2008-12-04 2010-06-10 Aethlon Medical, Inc. Affinity capture of circulating biomarkers
WO2010141862A2 (en) * 2009-06-05 2010-12-09 Mayo Foundation For Medical Education And Research Methods and materials for isolating exosomes
JP5667180B2 (ja) * 2009-07-01 2015-02-12 イオン メディックス インコーポレイテッド 哺乳類の有核細胞に由来するマイクロベシクル及びその用途
WO2012006476A2 (en) * 2010-07-07 2012-01-12 Aethlon Medical, Inc. Methods and compositions for quantifying exosomes
CA2817111C (en) * 2010-11-10 2019-03-05 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
US20140004601A1 (en) * 2010-12-20 2014-01-02 Agency For Science, Technology And Research Method of purifying exosomes
EP2721179A4 (en) * 2011-06-16 2014-10-01 Caris Life Sciences Luxembourg Holdings S A R L BIOMARKER COMPOSITIONS AND METHOD THEREFOR

Also Published As

Publication number Publication date
AU2014203987A1 (en) 2015-08-20
MX2015008727A (es) 2016-04-21
MX366153B (es) 2019-06-28
IL239786A0 (en) 2015-08-31
MX392552B (es) 2025-03-11
AU2018202789A1 (en) 2018-05-10
WO2014107571A1 (en) 2014-07-10
HK1217365A1 (en) 2017-01-06
CN110106229B (zh) 2023-03-28
IL267526B (en) 2020-10-29
IL267526A (en) 2019-08-29
EP3499212A1 (en) 2019-06-19
MX2019007742A (es) 2019-08-29
EP3499212B1 (en) 2020-08-12
EP2941629A4 (en) 2017-03-22
IL239786B (en) 2019-07-31
JP2016502862A (ja) 2016-02-01
CA2897207C (en) 2022-08-02
CA2897207A1 (en) 2014-07-10
US20150353920A1 (en) 2015-12-10
AU2014203987B2 (en) 2018-01-25
CN105026911B (zh) 2019-01-22
EP2941629B1 (en) 2018-10-10
CN105026911A (zh) 2015-11-04
EP2941629A1 (en) 2015-11-11
US20210171934A1 (en) 2021-06-10
AU2018202789B2 (en) 2020-01-02
BR112015016136A2 (pt) 2017-07-11
CN110106229A (zh) 2019-08-09

Similar Documents

Publication Publication Date Title
JP7128866B2 (ja) 微小小胞体を単離する方法、および生体試料から核酸を抽出する方法
US20210171934A1 (en) Methods for isolating microvesicles
US20210238582A1 (en) Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free dna from biofluids
US11268085B2 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples
HK40009837A (en) Methods for isolating microvesicles
HK1217365B (en) Methods for isolating microvesicles
HK1238676A1 (en) Methods for isolating microvesicles and extracting nucleic acids from biological samples

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20161102

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20170929

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20171031

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20180129

A524 Written submission of copy of amendment under article 19 pct

Free format text: JAPANESE INTERMEDIATE CODE: A524

Effective date: 20180424

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20180807

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20181016

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20181106

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20181206

R150 Certificate of patent or registration of utility model

Ref document number: 6449781

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250