JP6449781B2 - 微細小胞の単離方法 - Google Patents

微細小胞の単離方法 Download PDF

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JP6449781B2
JP6449781B2 JP2015551774A JP2015551774A JP6449781B2 JP 6449781 B2 JP6449781 B2 JP 6449781B2 JP 2015551774 A JP2015551774 A JP 2015551774A JP 2015551774 A JP2015551774 A JP 2015551774A JP 6449781 B2 JP6449781 B2 JP 6449781B2
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rna
microvesicles
plasma
filter
buffer
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JP2016502862A (ja
JP2016502862A5 (enExample
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エンダール ダニエル
エンダール ダニエル
ラマチャンドラン アパルナ
ラマチャンドラン アパルナ
ヤン ハオホゥオン
ヤン ハオホゥオン
バーグホフ エミリー
バーグホフ エミリー
ウェイ タイ−フェン
ウェイ タイ−フェン
ナアホルム ミゲル
ナアホルム ミゲル
カール オロフ スコグ ヨハン
カール オロフ スコグ ヨハン
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エクソサム ダイアグノスティクス，インコーポレイティド
エクソサム ダイアグノスティクス，インコーポレイティド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015551774A 2013-01-03 2014-01-03 微細小胞の単離方法 Active JP6449781B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361748575P 2013-01-03 2013-01-03
US61/748,575 2013-01-03
PCT/US2014/010173 WO2014107571A1 (en) 2013-01-03 2014-01-03 Methods for isolating microvesicles

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JP2016502862A JP2016502862A (ja) 2016-02-01
JP2016502862A5 JP2016502862A5 (enExample) 2018-06-14
JP6449781B2 true JP6449781B2 (ja) 2019-01-09

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US (2) US20150353920A1 (enExample)
EP (2) EP3499212B1 (enExample)
JP (1) JP6449781B2 (enExample)
CN (2) CN110106229B (enExample)
AU (2) AU2014203987B2 (enExample)
BR (1) BR112015016136A2 (enExample)
CA (1) CA2897207C (enExample)
IL (2) IL239786B (enExample)
MX (2) MX392552B (enExample)
WO (1) WO2014107571A1 (enExample)

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Publication number Publication date
IL239786A0 (en) 2015-08-31
US20210171934A1 (en) 2021-06-10
IL267526A (en) 2019-08-29
EP3499212A1 (en) 2019-06-19
MX2015008727A (es) 2016-04-21
IL267526B (en) 2020-10-29
MX392552B (es) 2025-03-11
BR112015016136A2 (pt) 2017-07-11
CA2897207A1 (en) 2014-07-10
HK1217365A1 (en) 2017-01-06
US20150353920A1 (en) 2015-12-10
WO2014107571A1 (en) 2014-07-10
AU2018202789B2 (en) 2020-01-02
MX2019007742A (es) 2019-08-29
CN110106229A (zh) 2019-08-09
MX366153B (es) 2019-06-28
AU2014203987A1 (en) 2015-08-20
AU2014203987B2 (en) 2018-01-25
CN105026911B (zh) 2019-01-22
EP3499212B1 (en) 2020-08-12
JP2016502862A (ja) 2016-02-01
CA2897207C (en) 2022-08-02
EP2941629A4 (en) 2017-03-22
EP2941629B1 (en) 2018-10-10
CN105026911A (zh) 2015-11-04
AU2018202789A1 (en) 2018-05-10
CN110106229B (zh) 2023-03-28
EP2941629A1 (en) 2015-11-11
IL239786B (en) 2019-07-31

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