JP6449781B2 - 微細小胞の単離方法 - Google Patents
微細小胞の単離方法 Download PDFInfo
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- JP6449781B2 JP6449781B2 JP2015551774A JP2015551774A JP6449781B2 JP 6449781 B2 JP6449781 B2 JP 6449781B2 JP 2015551774 A JP2015551774 A JP 2015551774A JP 2015551774 A JP2015551774 A JP 2015551774A JP 6449781 B2 JP6449781 B2 JP 6449781B2
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- rna
- microvesicles
- plasma
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361748575P | 2013-01-03 | 2013-01-03 | |
| US61/748,575 | 2013-01-03 | ||
| PCT/US2014/010173 WO2014107571A1 (en) | 2013-01-03 | 2014-01-03 | Methods for isolating microvesicles |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016502862A JP2016502862A (ja) | 2016-02-01 |
| JP2016502862A5 JP2016502862A5 (enExample) | 2018-06-14 |
| JP6449781B2 true JP6449781B2 (ja) | 2019-01-09 |
Family
ID=51062472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015551774A Active JP6449781B2 (ja) | 2013-01-03 | 2014-01-03 | 微細小胞の単離方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20150353920A1 (enExample) |
| EP (2) | EP3499212B1 (enExample) |
| JP (1) | JP6449781B2 (enExample) |
| CN (2) | CN110106229B (enExample) |
| AU (2) | AU2014203987B2 (enExample) |
| BR (1) | BR112015016136A2 (enExample) |
| CA (1) | CA2897207C (enExample) |
| IL (2) | IL239786B (enExample) |
| MX (2) | MX392552B (enExample) |
| WO (1) | WO2014107571A1 (enExample) |
Families Citing this family (57)
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| ES2744589T3 (es) * | 2011-05-11 | 2020-02-25 | Exosome Diagnostics Inc | Extracción de ácido nucleico de materiales biológicos heterogéneos |
| EP3467108B1 (en) * | 2013-03-14 | 2024-05-22 | Translate Bio, Inc. | Methods for purification of messenger rna |
| US20170165334A1 (en) * | 2015-12-11 | 2017-06-15 | Tianxin Wang | Methods to Treat Diseases with Protein, Peptide, Antigen Modification and Hemopurification |
| US11268085B2 (en) | 2014-05-27 | 2022-03-08 | Exosome Diagnostics, Inc. | Methods for isolating microvesicles and extracting nucleic acids from biological samples |
| CN113699143B (zh) | 2014-07-09 | 2024-08-06 | 外来体诊断公司 | 从生物样品中分离微泡和提取核酸的方法 |
| WO2016007951A1 (en) * | 2014-07-11 | 2016-01-14 | KARNAKIS, Jennifer A. | Compositions and methods for detecting rare sequence variants in nucleic acid sequencing |
| EP3209403A4 (en) * | 2014-10-24 | 2018-09-12 | Abbott Molecular Inc. | Enrichment of small nucleic acids |
| EP3112474A1 (en) | 2015-06-29 | 2017-01-04 | Evonik Degussa GmbH | Method of detecting avian necrotic enteritis |
| WO2017062901A2 (en) * | 2015-10-07 | 2017-04-13 | Tymora Analytical Operations Llc | Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis |
| SG11201809005TA (en) | 2016-04-15 | 2018-11-29 | Exosome Diagnostics Inc | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| CN105716923A (zh) * | 2016-04-21 | 2016-06-29 | 华南理工大学 | 一种聚合物囊泡扫描电镜样品的制备方法 |
| US11214833B2 (en) | 2016-05-05 | 2022-01-04 | Exosome Diagnostics, Inc. | Profiling microvesicle nucleic acids and uses thereof as signatures in diagnosis of renal transplant rejection |
| US10808240B2 (en) | 2016-05-13 | 2020-10-20 | Exosome Diagnostics, Inc. | Automated and manual methods for isolation of extracellular vesicles and co-isolation of cell-free DNA from biofluids |
| WO2018070479A1 (ja) | 2016-10-13 | 2018-04-19 | 合同会社みらか中央研究所 | 細胞外小胞の回収方法 |
| EP3529374B1 (en) | 2016-10-21 | 2024-04-03 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| EP3548635B1 (en) | 2016-11-30 | 2021-04-28 | Exosome Diagnostics, Inc. | Methods to detect mutations in plasma using exosomal rna and cell free dna from non-small cell lung cancer patients |
| CN110072994A (zh) * | 2016-12-23 | 2019-07-30 | 埃克波菲尔有限公司 | 用于纯化或分离微泡和外泌体的方法与组合物 |
| US20200149036A1 (en) | 2017-01-02 | 2020-05-14 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| KR101875594B1 (ko) * | 2017-01-13 | 2018-07-06 | ㈜로제타엑소좀 | 금속을 이용한 세포밖 소포체의 분리 방법 |
| BR112019023772A2 (pt) | 2017-05-12 | 2020-06-09 | Evonik Operations Gmbh | método para detectar doenças induzidas por c. perfringens em animais e kit de diagnóstico |
| JP7225121B2 (ja) | 2017-05-17 | 2023-02-20 | エクソサム ダイアグノスティクス，インコーポレイティド | 微小胞核酸および／またはタンパク質、並びに腎移植拒絶反応マーカーとしてのその使用 |
| CN107271655A (zh) * | 2017-05-18 | 2017-10-20 | 成都中医药大学附属医院 | 一种检测尿外泌体荷载miRNAs的试剂盒和方法 |
| WO2019013991A2 (en) * | 2017-07-12 | 2019-01-17 | Illumina, Inc. | METHODS, SYSTEMS AND MATERIALS FOR EXTRACTING NUCLEIC ACIDS |
| WO2019014486A1 (en) * | 2017-07-12 | 2019-01-17 | Exosome Diagnostics, Inc. | METHODS FOR ISOLATING AND ENRICHING POPULATIONS OF EXTRACELLULAR VESICLES DERIVED FROM BIOFLUIDS, AND METHODS OF USE THEREOF |
| WO2019018537A1 (en) * | 2017-07-18 | 2019-01-24 | Exosome Diagnostics, Inc. | NUCLEIC ACID SEQUENCING ASSOCIATED WITH EXOSOMAL ISOLATION IN PATIENTS WITH MULTIPLE GLIOBLASTOMA |
| US11904259B2 (en) * | 2017-07-26 | 2024-02-20 | Rosetta Exosome | Method for isolating extracellular vesicles using cations |
| WO2019132688A1 (ru) * | 2017-12-26 | 2019-07-04 | Общество С Ограниченной Ответственностью "Простагност" | Способ и устройство для выделения внеклеточных везикул из биологических жидкостей с помощью каскадной ультрафильтрации |
| US12031991B2 (en) | 2018-03-02 | 2024-07-09 | Evonik Operations Gmbh | In vitro method for detecting intestinal barrier failure in animals |
| WO2019236853A1 (en) | 2018-06-06 | 2019-12-12 | Exosome Diagnostics, Inc. | Methods for developing urine biomarkers and for detecting bladder cancer |
| CN112585446A (zh) | 2018-06-18 | 2021-03-30 | 埃克波菲尔有限公司 | 用于纯化或分离微泡和外泌体的方法与组合物 |
| CN108841777A (zh) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | 基于静电吸附的胞外囊泡及其内含物的提取方法及装置 |
| AU2019292939A1 (en) * | 2018-06-27 | 2021-01-07 | The Trustees Of Indiana University | Methods for analyzing DNA in urine |
| US10955379B2 (en) | 2018-09-27 | 2021-03-23 | Taiwan Semiconductor Manufacturing Co., Ltd. | Differential sensing with BioFET sensors |
| CN109337859A (zh) * | 2018-10-08 | 2019-02-15 | 南昌大学 | 一种分离细胞表面囊泡的方法 |
| US12038360B2 (en) * | 2018-10-30 | 2024-07-16 | Craif Inc. | Device to be used for capturing extracellular vesicles, and preservation method and transport method for extracellular vesicles |
| WO2020106853A1 (en) | 2018-11-20 | 2020-05-28 | Exosome Diagnostics, Inc. | Compositions and methods for internal controls of microvesicle isolations |
| JP2020092688A (ja) * | 2018-12-12 | 2020-06-18 | 国立大学法人東海国立大学機構 | マイクロｒｎａを含む体液抽出物 |
| EP3894863B1 (en) | 2018-12-14 | 2022-11-23 | Evonik Operations GmbH | In vitro method for detecting avian intestinal dysbiosis |
| WO2020180741A1 (en) | 2019-03-01 | 2020-09-10 | Mercy Bioanalytics, Inc. | Systems, compositions, and methods for target entity detection |
| EP4671268A2 (en) | 2019-03-21 | 2025-12-31 | Lonza Sales AG | METHOD FOR PREPARING EXTRACELLULAR VESICLES |
| CN112048462B (zh) * | 2019-06-05 | 2022-08-23 | 北京丰特云基科技发展有限公司 | 一种基于阴离子多聚物修饰基质的细胞外囊泡分离富集方法 |
| US12493029B2 (en) | 2019-09-18 | 2025-12-09 | Exosome Diagnostics, Inc. | Compositions, methods, and kits for the isolation of extracellular vesicles |
| US10984211B1 (en) | 2019-10-18 | 2021-04-20 | Taiwan Semiconductor Manufacturing Co., Ltd. | Semiconductor device with bioFET and biometric sensors |
| CN114761556A (zh) * | 2019-12-16 | 2022-07-15 | 凯杰有限公司 | 富集囊泡rna的方法 |
| EP4077659A1 (en) | 2019-12-16 | 2022-10-26 | QIAGEN GmbH | Enrichment method |
| JP7425403B2 (ja) * | 2020-02-17 | 2024-01-31 | 株式会社Ｊｖｃケンウッド | 生体試料分析方法 |
| CN113495029A (zh) * | 2020-04-01 | 2021-10-12 | 杨昆德 | 用于分离欲求物质的装置和方法 |
| US20230168162A1 (en) * | 2020-04-24 | 2023-06-01 | Korea University Research And Business Foundation | Microvesicle isolation method and microvesicle isolation |
| WO2021243206A1 (en) | 2020-05-29 | 2021-12-02 | Exosome Diagnostics, Inc. | Use of microvesicle signature for the diagnosis and treatment of kidney transplant rejection |
| CA3193107A1 (en) * | 2020-09-23 | 2022-03-31 | Codiak Biosciences, Inc. | Process for preparing extracellular vesicles |
| CN112391382B (zh) * | 2020-12-07 | 2022-10-11 | 湖北盛齐安生物科技股份有限公司 | 一种快速提取囊泡dna的方法 |
| JP7773765B2 (ja) * | 2020-12-18 | 2025-11-20 | 国立研究開発法人産業技術総合研究所 | 回収用キットおよび核酸含有溶液の調製方法 |
| WO2023288130A1 (en) | 2021-07-16 | 2023-01-19 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| AU2023221644A1 (en) | 2022-02-18 | 2024-09-12 | Bio-Techne Corporation | Use of microvesicle signatures in the identification and treatment of renal disorders |
| EP4584397A1 (en) | 2022-09-07 | 2025-07-16 | Exosome Diagnostics, Inc. | Methods of detecting sjögren's syndrome using salivary exosomes |
| WO2025057787A1 (ja) * | 2023-09-14 | 2025-03-20 | 東洋紡株式会社 | 細胞外小胞の製造方法 |
| CN117487739B (zh) * | 2023-10-30 | 2024-10-01 | 深圳市茵冠生物科技有限公司 | 一种大规模纯化293细胞外泌体的方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US4935342A (en) * | 1986-12-01 | 1990-06-19 | Syngene, Inc. | Method of isolating and purifying nucleic acids from biological samples |
| US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
| FR2788780B1 (fr) | 1999-01-27 | 2001-03-30 | Ap Cells Inc | Procede de preparation de vesicules membranaires |
| GB9927320D0 (en) | 1999-11-18 | 2000-01-12 | Chiron Spa | Exosome separation |
| US6812023B1 (en) | 2000-04-27 | 2004-11-02 | Anosys, Inc. | Methods of producing membrane vesicles |
| JP2004105009A (ja) * | 2002-09-13 | 2004-04-08 | Dojindo Laboratories | テトラフェニルホウ素化合物から成る核酸分離用試薬とそれを用いる核酸分離方法 |
| EP3708682B1 (en) | 2008-02-01 | 2022-11-23 | The General Hospital Corporation | Use of microvesicles in diagnosis and prognosis of medical diseases and conditions |
| WO2010065765A2 (en) * | 2008-12-04 | 2010-06-10 | Aethlon Medical, Inc. | Affinity capture of circulating biomarkers |
| US20120077263A1 (en) * | 2009-06-05 | 2012-03-29 | Mayo Foundation For Medical Education And Research | Methods and materials for isolating exosomes |
| CN102596177B (zh) * | 2009-07-01 | 2014-05-28 | 阿昂梅迪克斯公司 | 来源于有核哺乳动物细胞的微囊泡及其应用 |
| ES2624284T3 (es) * | 2010-07-07 | 2017-07-13 | Aethlon Medical Inc | Métodos para cuantificar exosomas |
| JP6219721B2 (ja) * | 2010-11-10 | 2017-10-25 | エキソソーム ダイアグノスティックス インコーポレイテッド | 核酸含有粒子の単離および該粒子からの核酸の抽出のための方法 |
| US20140004601A1 (en) * | 2010-12-20 | 2014-01-02 | Agency For Science, Technology And Research | Method of purifying exosomes |
| EP2721179A4 (en) * | 2011-06-16 | 2014-10-01 | Caris Life Sciences Luxembourg Holdings S A R L | BIOMARKER COMPOSITIONS AND METHOD THEREFOR |
-
2014
- 2014-01-03 BR BR112015016136A patent/BR112015016136A2/pt not_active IP Right Cessation
- 2014-01-03 US US14/759,238 patent/US20150353920A1/en not_active Abandoned
- 2014-01-03 EP EP18199291.8A patent/EP3499212B1/en active Active
- 2014-01-03 JP JP2015551774A patent/JP6449781B2/ja active Active
- 2014-01-03 CN CN201910000819.0A patent/CN110106229B/zh active Active
- 2014-01-03 EP EP14735397.3A patent/EP2941629B1/en active Active
- 2014-01-03 AU AU2014203987A patent/AU2014203987B2/en active Active
- 2014-01-03 MX MX2019007742A patent/MX392552B/es unknown
- 2014-01-03 CN CN201480002901.2A patent/CN105026911B/zh active Active
- 2014-01-03 MX MX2015008727A patent/MX366153B/es active IP Right Grant
- 2014-01-03 CA CA2897207A patent/CA2897207C/en active Active
- 2014-01-03 WO PCT/US2014/010173 patent/WO2014107571A1/en not_active Ceased
-
2015
- 2015-07-05 IL IL239786A patent/IL239786B/en active IP Right Grant
-
2018
- 2018-04-20 AU AU2018202789A patent/AU2018202789B2/en active Active
-
2019
- 2019-06-19 IL IL267526A patent/IL267526B/en active IP Right Grant
-
2020
- 2020-06-05 US US16/893,966 patent/US20210171934A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20210171934A1 (en) | 2021-06-10 |
| AU2018202789B2 (en) | 2020-01-02 |
| CN110106229B (zh) | 2023-03-28 |
| EP2941629A1 (en) | 2015-11-11 |
| MX2019007742A (es) | 2019-08-29 |
| CN105026911A (zh) | 2015-11-04 |
| IL267526A (en) | 2019-08-29 |
| CN110106229A (zh) | 2019-08-09 |
| US20150353920A1 (en) | 2015-12-10 |
| HK1217365A1 (en) | 2017-01-06 |
| AU2014203987B2 (en) | 2018-01-25 |
| CN105026911B (zh) | 2019-01-22 |
| IL239786B (en) | 2019-07-31 |
| EP2941629B1 (en) | 2018-10-10 |
| MX2015008727A (es) | 2016-04-21 |
| JP2016502862A (ja) | 2016-02-01 |
| EP3499212A1 (en) | 2019-06-19 |
| WO2014107571A1 (en) | 2014-07-10 |
| CA2897207A1 (en) | 2014-07-10 |
| IL267526B (en) | 2020-10-29 |
| IL239786A0 (en) | 2015-08-31 |
| EP2941629A4 (en) | 2017-03-22 |
| AU2014203987A1 (en) | 2015-08-20 |
| BR112015016136A2 (pt) | 2017-07-11 |
| AU2018202789A1 (en) | 2018-05-10 |
| MX366153B (es) | 2019-06-28 |
| MX392552B (es) | 2025-03-11 |
| CA2897207C (en) | 2022-08-02 |
| EP3499212B1 (en) | 2020-08-12 |
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