JP2014507155A5 - - Google Patents
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- Publication number
- JP2014507155A5 JP2014507155A5 JP2013554672A JP2013554672A JP2014507155A5 JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5 JP 2013554672 A JP2013554672 A JP 2013554672A JP 2013554672 A JP2013554672 A JP 2013554672A JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- solution
- beads
- washing
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 239000011324 bead Substances 0.000 claims 20
- 238000000034 method Methods 0.000 claims 16
- 238000000746 purification Methods 0.000 claims 16
- 238000005406 washing Methods 0.000 claims 14
- 239000011159 matrix material Substances 0.000 claims 8
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 4
- 239000003960 organic solvent Substances 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 238000011156 evaluation Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 239000012488 sample solution Substances 0.000 claims 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161444952P | 2011-02-21 | 2011-02-21 | |
| US61/444,952 | 2011-02-21 | ||
| PCT/US2012/025861 WO2012115926A2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014507155A JP2014507155A (ja) | 2014-03-27 |
| JP2014507155A5 true JP2014507155A5 (enExample) | 2015-04-09 |
| JP6113083B2 JP6113083B2 (ja) | 2017-04-19 |
Family
ID=46653044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013554672A Expired - Fee Related JP6113083B2 (ja) | 2011-02-21 | 2012-02-21 | マイクロ流体素子を基礎とした核酸精製方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8722329B2 (enExample) |
| EP (1) | EP2678429B1 (enExample) |
| JP (1) | JP6113083B2 (enExample) |
| AU (1) | AU2012220825B2 (enExample) |
| WO (1) | WO2012115926A2 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8722329B2 (en) * | 2011-02-21 | 2014-05-13 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| CN108473983B (zh) | 2015-12-28 | 2022-09-13 | 皇家飞利浦有限公司 | 使用单一洗涤和洗脱缓冲溶液的核酸纯化系统 |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| CN110998333B (zh) * | 2017-05-16 | 2023-08-25 | Sk电信有限公司 | 使用盒的核酸分析设备 |
| DE112019004459T5 (de) * | 2018-09-05 | 2021-07-15 | Adana Bilim Ve Teknoloji Universitesi | Anreicherung von proben mittels magnetischer partikel in mikrokanälen |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN115820412B (zh) * | 2022-11-17 | 2025-12-02 | 北京昌平实验室 | 一种从复杂样本中特异检测核酸的微流控卡盒及方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0245945B1 (en) * | 1986-04-11 | 1993-11-10 | Applied Biosystems, Inc. | Automated nucleic acid extractor |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US6037465A (en) | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| EP1071691B1 (de) | 1998-02-04 | 2005-09-28 | MERCK PATENT GmbH | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| ES2294287T5 (es) | 2002-01-08 | 2015-10-27 | F. Hoffmann-La Roche Ag | Empleo de un material de sílice en una reacción de amplificación |
| DE60323579D1 (de) * | 2002-07-29 | 2008-10-30 | Jsr Corp | Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| EP1794581A2 (en) * | 2004-09-15 | 2007-06-13 | Microchip Biotechnologies, Inc. | Microfluidic devices |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20100063263A1 (en) | 2005-06-23 | 2010-03-11 | Siemens Medical Solutions Diagnostics Gmbh | Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use |
| US7976795B2 (en) * | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| US20100190240A1 (en) | 2007-03-21 | 2010-07-29 | Ibis Biosciences, Inc. | Reagents for nucleic acid purification |
| JP5523327B2 (ja) * | 2007-10-12 | 2014-06-18 | レオニックス，インコーポレイテッド | 統合型マイクロ流体デバイスおよび方法 |
| WO2009108260A2 (en) * | 2008-01-22 | 2009-09-03 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
| JP5782384B2 (ja) | 2009-02-03 | 2015-09-24 | ネットバイオ・インコーポレーテッドＮｅｔＢｉｏ， Ｉｎｃ． | 未処理試料から核酸を単離するための自己完結型装置および精製方法 |
| US20120164036A1 (en) * | 2009-07-21 | 2012-06-28 | Seth Stern | Microfluidic devices and uses thereof |
| US8722329B2 (en) * | 2011-02-21 | 2014-05-13 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
-
2012
- 2012-02-21 US US13/400,857 patent/US8722329B2/en active Active
- 2012-02-21 EP EP12749025.8A patent/EP2678429B1/en active Active
- 2012-02-21 JP JP2013554672A patent/JP6113083B2/ja not_active Expired - Fee Related
- 2012-02-21 AU AU2012220825A patent/AU2012220825B2/en not_active Ceased
- 2012-02-21 WO PCT/US2012/025861 patent/WO2012115926A2/en not_active Ceased
-
2014
- 2014-03-26 US US14/226,204 patent/US9169479B2/en active Active
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