JP2014507155A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2014507155A5 JP2014507155A5 JP2013554672A JP2013554672A JP2014507155A5 JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5 JP 2013554672 A JP2013554672 A JP 2013554672A JP 2013554672 A JP2013554672 A JP 2013554672A JP 2014507155 A5 JP2014507155 A5 JP 2014507155A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- solution
- beads
- washing
- nucleic acids
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical class 0.000 claims 31
- 102000039446 nucleic acids Human genes 0.000 claims 31
- 108020004707 nucleic acids Proteins 0.000 claims 31
- 239000011324 bead Substances 0.000 claims 20
- 238000000034 method Methods 0.000 claims 16
- 238000000746 purification Methods 0.000 claims 16
- 238000005406 washing Methods 0.000 claims 14
- 239000011159 matrix material Substances 0.000 claims 8
- 239000003153 chemical reaction reagent Substances 0.000 claims 4
- 238000010828 elution Methods 0.000 claims 4
- 239000007788 liquid Substances 0.000 claims 4
- 239000003960 organic solvent Substances 0.000 claims 4
- 230000003321 amplification Effects 0.000 claims 2
- 238000004458 analytical method Methods 0.000 claims 2
- 238000011156 evaluation Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 239000000523 sample Substances 0.000 claims 2
- 239000012488 sample solution Substances 0.000 claims 2
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161444952P | 2011-02-21 | 2011-02-21 | |
| US61/444,952 | 2011-02-21 | ||
| PCT/US2012/025861 WO2012115926A2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014507155A JP2014507155A (ja) | 2014-03-27 |
| JP2014507155A5 true JP2014507155A5 (enExample) | 2015-04-09 |
| JP6113083B2 JP6113083B2 (ja) | 2017-04-19 |
Family
ID=46653044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013554672A Expired - Fee Related JP6113083B2 (ja) | 2011-02-21 | 2012-02-21 | マイクロ流体素子を基礎とした核酸精製方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8722329B2 (enExample) |
| EP (1) | EP2678429B1 (enExample) |
| JP (1) | JP6113083B2 (enExample) |
| AU (1) | AU2012220825B2 (enExample) |
| WO (1) | WO2012115926A2 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012220825B2 (en) * | 2011-02-21 | 2015-12-17 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| EP3397763B1 (en) | 2015-12-28 | 2020-10-28 | Koninklijke Philips N.V. | Nucleic acid purification system using a single wash and elution buffer solution |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| CN110998333B (zh) * | 2017-05-16 | 2023-08-25 | Sk电信有限公司 | 使用盒的核酸分析设备 |
| GB2591406B (en) * | 2018-09-05 | 2023-03-01 | Ihsan Dogramaci Bilkent Univ | Enrichment of samples inside the microchannels by using magnetic particles |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN115820412B (zh) * | 2022-11-17 | 2025-12-02 | 北京昌平实验室 | 一种从复杂样本中特异检测核酸的微流控卡盒及方法 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0245945B1 (en) * | 1986-04-11 | 1993-11-10 | Applied Biosystems, Inc. | Automated nucleic acid extractor |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US6037465A (en) | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| DE59912604D1 (de) | 1998-02-04 | 2005-11-03 | Merck Patent Gmbh | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| WO2003057910A2 (en) | 2002-01-08 | 2003-07-17 | Roche Diagnostics Gmbh | Use of silica material in an amplification reaction |
| US20040029166A1 (en) * | 2002-07-29 | 2004-02-12 | Jsr Corporation | Nucleic acid-separating method and nucleic acid-extracting reagent |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| CN102759466A (zh) * | 2004-09-15 | 2012-10-31 | 英特基因有限公司 | 微流体装置 |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20100063263A1 (en) | 2005-06-23 | 2010-03-11 | Siemens Medical Solutions Diagnostics Gmbh | Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use |
| US7976795B2 (en) * | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| EP2132311A1 (en) | 2007-03-21 | 2009-12-16 | Ibis Biosciences, Inc. | Reagents for nucleic acid purification |
| WO2009049268A1 (en) * | 2007-10-12 | 2009-04-16 | Rheonix, Inc. | Integrated microfluidic device and methods |
| WO2009108260A2 (en) * | 2008-01-22 | 2009-09-03 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
| AU2010210666C1 (en) * | 2009-02-03 | 2016-01-28 | Ande Corporation | Nucleic acid purification |
| WO2011011172A1 (en) * | 2009-07-21 | 2011-01-27 | IntegenX, Inc. | Microfluidic devices and uses thereof |
| AU2012220825B2 (en) * | 2011-02-21 | 2015-12-17 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
-
2012
- 2012-02-21 AU AU2012220825A patent/AU2012220825B2/en not_active Ceased
- 2012-02-21 EP EP12749025.8A patent/EP2678429B1/en active Active
- 2012-02-21 WO PCT/US2012/025861 patent/WO2012115926A2/en not_active Ceased
- 2012-02-21 JP JP2013554672A patent/JP6113083B2/ja not_active Expired - Fee Related
- 2012-02-21 US US13/400,857 patent/US8722329B2/en active Active
-
2014
- 2014-03-26 US US14/226,204 patent/US9169479B2/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Rogacs et al. | Purification of nucleic acids using isotachophoresis | |
| JP6797185B2 (ja) | 核酸を自動的に抽出する装置および方法 | |
| JP5876481B2 (ja) | 超高速核酸精製方法 | |
| JP2013172736A5 (enExample) | ||
| CN103890176B (zh) | 制备生物材料的方法 | |
| DE60137323D1 (de) | System und verfahren zur behandlung von magnetpartikeln in test-flüssigkeiten zum sammeln von dna und rna | |
| CN107257857B (zh) | 用于ivt后rna纯化的方法和试剂盒 | |
| CN111057704B (zh) | 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法 | |
| CN103614371A (zh) | 一种同时提取血清、双拭子中动物dna病毒和rna病毒核酸的方法 | |
| Purdy | Nucleic acid recovery from complex environmental samples | |
| Ali et al. | Integration of nucleic acid extraction protocol with automated extractor for multiplex viral detection | |
| JP2013543370A5 (enExample) | ||
| CN105733927A (zh) | 一种用于核酸提取的耗材及多联耗材 | |
| Hu et al. | Improve sample preparation process for miRNA isolation from the culture cells by using silica fiber membrane | |
| JP6113083B2 (ja) | マイクロ流体素子を基礎とした核酸精製方法 | |
| CN103114147B (zh) | 无固定点靶物质的适配体筛选方法 | |
| CN105420818A (zh) | 基于磁珠绑定的二代测序文库快速构建方法 | |
| CN115768887A (zh) | 一种单链rna纯化方法 | |
| CN106754890B (zh) | 一种病毒rna的提取试剂盒和提取方法 | |
| FI3395423T3 (fi) | Vuotaneen affiniteettipuhdistusligandin poistaminen | |
| CN106701737A (zh) | 一种具有片段选择性纯化能力的高效dna纯化磁珠试剂 | |
| JP2015515969A5 (enExample) | ||
| WO2009100172A1 (en) | Isolation of dna, rna and protein from a single sample | |
| Das et al. | A quick and cost-effective method for DNA-free total RNA isolation using magnetic silica beads | |
| JP2021166548A (ja) | 核の保存方法 |