AU2012220825B2 - Microfluidic device-based nucleic acid purification method - Google Patents
Microfluidic device-based nucleic acid purification method Download PDFInfo
- Publication number
- AU2012220825B2 AU2012220825B2 AU2012220825A AU2012220825A AU2012220825B2 AU 2012220825 B2 AU2012220825 B2 AU 2012220825B2 AU 2012220825 A AU2012220825 A AU 2012220825A AU 2012220825 A AU2012220825 A AU 2012220825A AU 2012220825 B2 AU2012220825 B2 AU 2012220825B2
- Authority
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- Australia
- Prior art keywords
- nucleic acid
- solution
- purification
- beads
- microfluidic device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161444952P | 2011-02-21 | 2011-02-21 | |
| US61/444,952 | 2011-02-21 | ||
| PCT/US2012/025861 WO2012115926A2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012220825A1 AU2012220825A1 (en) | 2013-08-08 |
| AU2012220825B2 true AU2012220825B2 (en) | 2015-12-17 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012220825A Ceased AU2012220825B2 (en) | 2011-02-21 | 2012-02-21 | Microfluidic device-based nucleic acid purification method |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8722329B2 (enExample) |
| EP (1) | EP2678429B1 (enExample) |
| JP (1) | JP6113083B2 (enExample) |
| AU (1) | AU2012220825B2 (enExample) |
| WO (1) | WO2012115926A2 (enExample) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8722329B2 (en) * | 2011-02-21 | 2014-05-13 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| US9803237B2 (en) | 2012-04-24 | 2017-10-31 | California Institute Of Technology | Slip-induced compartmentalization |
| CN108473983B (zh) | 2015-12-28 | 2022-09-13 | 皇家飞利浦有限公司 | 使用单一洗涤和洗脱缓冲溶液的核酸纯化系统 |
| DE102017206155A1 (de) * | 2017-04-11 | 2018-10-11 | Robert Bosch Gmbh | Desorption von Nukleinsäuren |
| CN110998333B (zh) * | 2017-05-16 | 2023-08-25 | Sk电信有限公司 | 使用盒的核酸分析设备 |
| DE112019004459T5 (de) * | 2018-09-05 | 2021-07-15 | Adana Bilim Ve Teknoloji Universitesi | Anreicherung von proben mittels magnetischer partikel in mikrokanälen |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN115820412B (zh) * | 2022-11-17 | 2025-12-02 | 北京昌平实验室 | 一种从复杂样本中特异检测核酸的微流控卡盒及方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090253181A1 (en) * | 2008-01-22 | 2009-10-08 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0245945B1 (en) * | 1986-04-11 | 1993-11-10 | Applied Biosystems, Inc. | Automated nucleic acid extractor |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US6037465A (en) | 1994-06-14 | 2000-03-14 | Invitek Gmbh | Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| EP1071691B1 (de) | 1998-02-04 | 2005-09-28 | MERCK PATENT GmbH | Verfahren zur isolierung und aufreinigung von nucleinsäuren |
| DE19856064C2 (de) | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
| ES2294287T5 (es) | 2002-01-08 | 2015-10-27 | F. Hoffmann-La Roche Ag | Empleo de un material de sílice en una reacción de amplificación |
| DE60323579D1 (de) * | 2002-07-29 | 2008-10-30 | Jsr Corp | Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz |
| CA2482097C (en) | 2003-10-13 | 2012-02-21 | F. Hoffmann-La Roche Ag | Methods for isolating nucleic acids |
| EP1794581A2 (en) * | 2004-09-15 | 2007-06-13 | Microchip Biotechnologies, Inc. | Microfluidic devices |
| EP1690938A1 (de) | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| US20100063263A1 (en) | 2005-06-23 | 2010-03-11 | Siemens Medical Solutions Diagnostics Gmbh | Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use |
| US7976795B2 (en) * | 2006-01-19 | 2011-07-12 | Rheonix, Inc. | Microfluidic systems |
| WO2007092713A2 (en) * | 2006-02-02 | 2007-08-16 | Trustees Of The University Of Pennsylvania | Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease |
| US20100190240A1 (en) | 2007-03-21 | 2010-07-29 | Ibis Biosciences, Inc. | Reagents for nucleic acid purification |
| JP5523327B2 (ja) * | 2007-10-12 | 2014-06-18 | レオニックス，インコーポレイテッド | 統合型マイクロ流体デバイスおよび方法 |
| JP5782384B2 (ja) | 2009-02-03 | 2015-09-24 | ネットバイオ・インコーポレーテッドＮｅｔＢｉｏ， Ｉｎｃ． | 未処理試料から核酸を単離するための自己完結型装置および精製方法 |
| US20120164036A1 (en) * | 2009-07-21 | 2012-06-28 | Seth Stern | Microfluidic devices and uses thereof |
| US8722329B2 (en) * | 2011-02-21 | 2014-05-13 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
-
2012
- 2012-02-21 US US13/400,857 patent/US8722329B2/en active Active
- 2012-02-21 EP EP12749025.8A patent/EP2678429B1/en active Active
- 2012-02-21 JP JP2013554672A patent/JP6113083B2/ja not_active Expired - Fee Related
- 2012-02-21 AU AU2012220825A patent/AU2012220825B2/en not_active Ceased
- 2012-02-21 WO PCT/US2012/025861 patent/WO2012115926A2/en not_active Ceased
-
2014
- 2014-03-26 US US14/226,204 patent/US9169479B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090253181A1 (en) * | 2008-01-22 | 2009-10-08 | Microchip Biotechnologies, Inc. | Universal sample preparation system and use in an integrated analysis system |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012115926A3 (en) | 2013-03-21 |
| US9169479B2 (en) | 2015-10-27 |
| EP2678429A4 (en) | 2014-10-29 |
| WO2012115926A2 (en) | 2012-08-30 |
| US20120214168A1 (en) | 2012-08-23 |
| JP6113083B2 (ja) | 2017-04-19 |
| US20140206009A1 (en) | 2014-07-24 |
| JP2014507155A (ja) | 2014-03-27 |
| EP2678429B1 (en) | 2018-04-18 |
| EP2678429A2 (en) | 2014-01-01 |
| US8722329B2 (en) | 2014-05-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) | ||
| MK14 | Patent ceased section 143(a) (annual fees not paid) or expired |