JP6113083B2 - マイクロ流体素子を基礎とした核酸精製方法 - Google Patents

マイクロ流体素子を基礎とした核酸精製方法 Download PDF

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JP6113083B2
JP6113083B2 JP2013554672A JP2013554672A JP6113083B2 JP 6113083 B2 JP6113083 B2 JP 6113083B2 JP 2013554672 A JP2013554672 A JP 2013554672A JP 2013554672 A JP2013554672 A JP 2013554672A JP 6113083 B2 JP6113083 B2 JP 6113083B2
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nucleic acid
solution
beads
washing
microfluidic device
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JP2014507155A5 (enExample
JP2014507155A (ja
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リンカーン・シー・ヤング
ジョウ・ポン
グウェンドリン・スピズ
ルビーナ・ヤスミン
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Rheonix Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
JP2013554672A 2011-02-21 2012-02-21 マイクロ流体素子を基礎とした核酸精製方法 Expired - Fee Related JP6113083B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161444952P 2011-02-21 2011-02-21
US61/444,952 2011-02-21
PCT/US2012/025861 WO2012115926A2 (en) 2011-02-21 2012-02-21 Microfluidic device-based nucleic acid purification method

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JP2014507155A JP2014507155A (ja) 2014-03-27
JP2014507155A5 JP2014507155A5 (enExample) 2015-04-09
JP6113083B2 true JP6113083B2 (ja) 2017-04-19

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US (2) US8722329B2 (enExample)
EP (1) EP2678429B1 (enExample)
JP (1) JP6113083B2 (enExample)
AU (1) AU2012220825B2 (enExample)
WO (1) WO2012115926A2 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8722329B2 (en) * 2011-02-21 2014-05-13 Rheonix, Inc. Microfluidic device-based nucleic acid purification method
US9803237B2 (en) 2012-04-24 2017-10-31 California Institute Of Technology Slip-induced compartmentalization
CN108473983B (zh) 2015-12-28 2022-09-13 皇家飞利浦有限公司 使用单一洗涤和洗脱缓冲溶液的核酸纯化系统
DE102017206155A1 (de) * 2017-04-11 2018-10-11 Robert Bosch Gmbh Desorption von Nukleinsäuren
CN110998333B (zh) * 2017-05-16 2023-08-25 Sk电信有限公司 使用盒的核酸分析设备
DE112019004459T5 (de) * 2018-09-05 2021-07-15 Adana Bilim Ve Teknoloji Universitesi Anreicherung von proben mittels magnetischer partikel in mikrokanälen
IT201900008334A1 (it) 2019-06-07 2020-12-07 Nurex Srl Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici
CN115820412B (zh) * 2022-11-17 2025-12-02 北京昌平实验室 一种从复杂样本中特异检测核酸的微流控卡盒及方法

Family Cites Families (21)

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EP0245945B1 (en) * 1986-04-11 1993-11-10 Applied Biosystems, Inc. Automated nucleic acid extractor
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US6037465A (en) 1994-06-14 2000-03-14 Invitek Gmbh Universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated various starting materials
JP2003144149A (ja) * 1997-03-31 2003-05-20 Takara Holdings Inc 分離装置及びその使用方法
FR2773818B1 (fr) * 1998-01-21 2000-02-18 Pasteur Merieux Serums Vacc Procede et dispositif de lyse de bacteries
EP1071691B1 (de) 1998-02-04 2005-09-28 MERCK PATENT GmbH Verfahren zur isolierung und aufreinigung von nucleinsäuren
DE19856064C2 (de) 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
ES2294287T5 (es) 2002-01-08 2015-10-27 F. Hoffmann-La Roche Ag Empleo de un material de sílice en una reacción de amplificación
DE60323579D1 (de) * 2002-07-29 2008-10-30 Jsr Corp Methode zur Separierung von Nukleinsäuren und dafür verwendbare Reagenz
CA2482097C (en) 2003-10-13 2012-02-21 F. Hoffmann-La Roche Ag Methods for isolating nucleic acids
EP1794581A2 (en) * 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
EP1690938A1 (de) 2005-02-11 2006-08-16 Qiagen GmbH Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden
US20100063263A1 (en) 2005-06-23 2010-03-11 Siemens Medical Solutions Diagnostics Gmbh Magnetic particles with a closed ultrathin silica layer, method for the production thereof and their use
US7976795B2 (en) * 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007092713A2 (en) * 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
US20100190240A1 (en) 2007-03-21 2010-07-29 Ibis Biosciences, Inc. Reagents for nucleic acid purification
JP5523327B2 (ja) * 2007-10-12 2014-06-18 レオニックス，インコーポレイテッド 統合型マイクロ流体デバイスおよび方法
WO2009108260A2 (en) * 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
JP5782384B2 (ja) 2009-02-03 2015-09-24 ネットバイオ・インコーポレーテッドＮｅｔＢｉｏ， Ｉｎｃ． 未処理試料から核酸を単離するための自己完結型装置および精製方法
US20120164036A1 (en) * 2009-07-21 2012-06-28 Seth Stern Microfluidic devices and uses thereof
US8722329B2 (en) * 2011-02-21 2014-05-13 Rheonix, Inc. Microfluidic device-based nucleic acid purification method

Also Published As

Publication number Publication date
WO2012115926A3 (en) 2013-03-21
US9169479B2 (en) 2015-10-27
EP2678429A4 (en) 2014-10-29
WO2012115926A2 (en) 2012-08-30
AU2012220825B2 (en) 2015-12-17
US20120214168A1 (en) 2012-08-23
US20140206009A1 (en) 2014-07-24
JP2014507155A (ja) 2014-03-27
EP2678429B1 (en) 2018-04-18
EP2678429A2 (en) 2014-01-01
US8722329B2 (en) 2014-05-13

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