JP2014517692A5 - - Google Patents
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- JP2014517692A5 JP2014517692A5 JP2014511591A JP2014511591A JP2014517692A5 JP 2014517692 A5 JP2014517692 A5 JP 2014517692A5 JP 2014511591 A JP2014511591 A JP 2014511591A JP 2014511591 A JP2014511591 A JP 2014511591A JP 2014517692 A5 JP2014517692 A5 JP 2014517692A5
- Authority
- JP
- Japan
- Prior art keywords
- hns
- composition
- item
- column
- bulk
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
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- 108010089932 heparan sulfate sulfatase Proteins 0.000 claims description 55
- 238000000034 method Methods 0.000 claims description 49
- 239000000203 mixture Substances 0.000 claims description 30
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 24
- 239000012539 chromatography resin Substances 0.000 claims description 19
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 238000005571 anion exchange chromatography Methods 0.000 claims description 8
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims description 7
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims description 7
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 239000007983 Tris buffer Substances 0.000 claims description 5
- 241001460678 Napo <wasp> Species 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 238000005277 cation exchange chromatography Methods 0.000 claims description 4
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 2
- 230000002209 hydrophobic effect Effects 0.000 claims 4
- 230000003993 interaction Effects 0.000 claims 4
- 238000000746 purification Methods 0.000 claims 3
- 239000007858 starting material Substances 0.000 claims 3
- 208000002678 Mucopolysaccharidoses Diseases 0.000 claims 2
- 238000005349 anion exchange Methods 0.000 claims 2
- 238000005341 cation exchange Methods 0.000 claims 2
- 206010028093 mucopolysaccharidosis Diseases 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 238000010828 elution Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000003480 eluent Substances 0.000 description 5
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 4
- 235000017281 sodium acetate Nutrition 0.000 description 4
- 239000001632 sodium acetate Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- GNFTZDOKVXKIBK-UHFFFAOYSA-N 3-(2-methoxyethoxy)benzohydrazide Chemical compound COCCOC1=CC=CC(C(=O)NN)=C1 GNFTZDOKVXKIBK-UHFFFAOYSA-N 0.000 description 2
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- VBAOEVKQBLGWTH-UHFFFAOYSA-N 2-pyridin-4-ylethanethiol Chemical compound SCCC1=CC=NC=C1 VBAOEVKQBLGWTH-UHFFFAOYSA-N 0.000 description 1
- 239000012564 Q sepharose fast flow resin Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000011026 diafiltration Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161488090P | 2011-05-19 | 2011-05-19 | |
| US61/488,090 | 2011-05-19 | ||
| PCT/US2012/038630 WO2012159053A1 (en) | 2011-05-19 | 2012-05-18 | Process for purifying heparan-n-sulfatase |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016214041A Division JP2017029170A (ja) | 2011-05-19 | 2016-11-01 | ヘパラン−ｎ−スルファターゼを精製するためのプロセス |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014517692A JP2014517692A (ja) | 2014-07-24 |
| JP2014517692A5 true JP2014517692A5 (enExample) | 2015-07-02 |
Family
ID=47177365
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014511591A Ceased JP2014517692A (ja) | 2011-05-19 | 2012-05-18 | ヘパラン−ｎ−スルファターゼを精製するためのプロセス |
| JP2016214041A Pending JP2017029170A (ja) | 2011-05-19 | 2016-11-01 | ヘパラン−ｎ−スルファターゼを精製するためのプロセス |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016214041A Pending JP2017029170A (ja) | 2011-05-19 | 2016-11-01 | ヘパラン−ｎ−スルファターゼを精製するためのプロセス |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20120329133A1 (enExample) |
| EP (2) | EP2929892B1 (enExample) |
| JP (2) | JP2014517692A (enExample) |
| AR (1) | AR086445A1 (enExample) |
| CA (1) | CA2836599A1 (enExample) |
| HK (1) | HK1214960A1 (enExample) |
| TW (1) | TW201307563A (enExample) |
| WO (1) | WO2012159053A1 (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11584777B2 (en) | 2017-08-31 | 2023-02-21 | Green Cross Corporation | Method for purifying a sulfatase protein |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7572619B2 (en) * | 2000-03-22 | 2009-08-11 | Octagene Gmbh | Recombinant blood clotting factors |
| DK1501369T3 (en) * | 2002-04-26 | 2015-09-28 | Genentech Inc | Non-affinity purification of proteins |
| ATE494367T1 (de) * | 2002-12-18 | 2011-01-15 | Hoffmann La Roche | Rekombinante desoxyribonuclease i aus rinder- pankreas mit hoher spezifischer aktivität |
| AU2005211775B2 (en) * | 2004-02-06 | 2009-10-08 | Biomarin Pharmaceutical Inc. | Manufacture of highly phosphorylated lysosomal enzymes and uses thereof |
| SI1828239T1 (sl) * | 2004-12-10 | 2011-11-30 | Zymogenetics Inc | Proizvodnja fgf18 v prokariotskih gostiteljih |
| EP2141178A4 (en) * | 2007-04-02 | 2010-05-19 | Kyowa Hakko Kirin Co Ltd | PROCESS FOR PRODUCING ANTITHROMBIN COMPOSITION |
| EP2245145B1 (en) | 2008-01-18 | 2016-12-14 | BioMarin Pharmaceutical Inc. | Manufacture of active highly phosphorylated human lysosomal sulfatase enzymes and uses thereof |
| CA2805413A1 (en) * | 2010-06-25 | 2011-12-29 | Shire Human Genetic Therapies, Inc. | Methods and compositions for cns delivery of heparan n-sulfatase |
-
2012
- 2012-05-17 TW TW101117561A patent/TW201307563A/zh unknown
- 2012-05-18 EP EP15166651.8A patent/EP2929892B1/en active Active
- 2012-05-18 CA CA2836599A patent/CA2836599A1/en not_active Abandoned
- 2012-05-18 JP JP2014511591A patent/JP2014517692A/ja not_active Ceased
- 2012-05-18 AR ARP120101760A patent/AR086445A1/es unknown
- 2012-05-18 WO PCT/US2012/038630 patent/WO2012159053A1/en not_active Ceased
- 2012-05-18 US US13/475,568 patent/US20120329133A1/en not_active Abandoned
- 2012-05-18 EP EP12785579.9A patent/EP2709654A4/en not_active Withdrawn
-
2016
- 2016-03-15 HK HK16102969.5A patent/HK1214960A1/en unknown
- 2016-11-01 JP JP2016214041A patent/JP2017029170A/ja active Pending
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