CN110177617A - 高载量耐碱蛋白a磁珠及其使用方法 - Google Patents

高载量耐碱蛋白a磁珠及其使用方法 Download PDF

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CN110177617A
CN110177617A CN201880005785.8A CN201880005785A CN110177617A CN 110177617 A CN110177617 A CN 110177617A CN 201880005785 A CN201880005785 A CN 201880005785A CN 110177617 A CN110177617 A CN 110177617A
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resisting
albumin
alkaline
magnetic bead
carrying capacity
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望超
贺瑞娜
韩卫娟
钱红
白涛
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Nanjing Jinsirui Science and Technology Biology Corp
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    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Abstract

提供了一种高载量耐碱蛋白A磁珠，其在pH 2‑14环境下均可保持其化学稳定性，并且结合免疫球蛋白IgG的能力大于50mg/mL。还提供了一种纯化和/或检测免疫球蛋白的方法，包括让含有免疫球蛋白的样品与所述高载量耐碱蛋白A磁珠接触的步骤。前述所提供的耐碱蛋白A磁珠可实现免疫球蛋白的快速纯化，节省约80％的处理时间，总体纯化成本可下降50％。除此之外，该耐碱蛋白A磁珠具备高耐碱特性，可以使用原位清洁的碱性方法对使用后的磁珠进行再生。磁珠本身的快磁响应和较好的分散性，可快速实现磁珠富集、清洗和洗脱，方便实现自动化高通量和大样品量纯化。

Description

PCT国内申请，说明书已公开。

Claims (19)

  1. PCT国内申请，权利要求书已公开。
CN201880005785.8A 2017-01-04 2018-01-04 高载量耐碱蛋白a磁珠及其使用方法 Pending CN110177617A (zh)

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CN2017100058788 2017-01-04
CN201710005878 2017-01-04
PCT/CN2018/071397 WO2018127099A1 (zh) 2017-01-04 2018-01-04 高载量耐碱蛋白a磁珠及其使用方法

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US (1) US11577218B2 (zh)
EP (1) EP3578255B1 (zh)
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CN114377626A (zh) * 2021-12-29 2022-04-22 苏州百奥吉生物科技有限公司 一种新型复合多糖protein A磁珠及其制备方法
CN114570302A (zh) * 2022-03-24 2022-06-03 苏州华度生物科技有限公司 一种基于蛋白的磁性琼脂糖多孔微球及其制备方法
CN114591407A (zh) * 2021-12-21 2022-06-07 科迈思(兰州)生物技术有限公司 耐碱蛋白a变体及其应用
CN116165047A (zh) * 2023-04-22 2023-05-26 北京中检维康生物技术有限公司 一种净化黄曲霉毒素的免疫磁珠试剂盒、使用方法及其应用
WO2023134773A1 (zh) * 2022-01-17 2023-07-20 南京金斯瑞生物科技有限公司 纯化方法
CN114377626B (zh) * 2021-12-29 2024-05-17 苏州百奥吉生物科技有限公司 一种新型复合多糖protein A磁珠及其制备方法

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CN114591407B (zh) * 2021-12-21 2023-07-04 百林科(兰州)新材料有限公司 耐碱蛋白a变体及其应用
CN114377626A (zh) * 2021-12-29 2022-04-22 苏州百奥吉生物科技有限公司 一种新型复合多糖protein A磁珠及其制备方法
CN114377626B (zh) * 2021-12-29 2024-05-17 苏州百奥吉生物科技有限公司 一种新型复合多糖protein A磁珠及其制备方法
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CN114570302A (zh) * 2022-03-24 2022-06-03 苏州华度生物科技有限公司 一种基于蛋白的磁性琼脂糖多孔微球及其制备方法
CN116165047A (zh) * 2023-04-22 2023-05-26 北京中检维康生物技术有限公司 一种净化黄曲霉毒素的免疫磁珠试剂盒、使用方法及其应用

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