JP2016510593A5 - - Google Patents

Info

Publication number

JP2016510593A5

JP2016510593A5 JP2015560761A JP2015560761A JP2016510593A5 JP 2016510593 A5 JP2016510593 A5 JP 2016510593A5 JP 2015560761 A JP2015560761 A JP 2015560761A JP 2015560761 A JP2015560761 A JP 2015560761A JP 2016510593 A5 JP2016510593 A5 JP 2016510593A5

Authority

JP

Japan

Prior art keywords

helicases

polynucleotide

spacers

target polynucleotide

helicase

Prior art date

2014-01-22

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 108091033319 polynucleotide Proteins 0.000 claims 64
• 102000040430 polynucleotide Human genes 0.000 claims 64
• 239000002157 polynucleotide Substances 0.000 claims 64
• 108060004795 Methyltransferase Proteins 0.000 claims 57
• 125000006850 spacer group Chemical group 0.000 claims 46
• 238000000034 method Methods 0.000 claims 25
• 239000011148 porous material Substances 0.000 claims 18
• 238000005259 measurement Methods 0.000 claims 6
• 239000002773 nucleotide Chemical group 0.000 claims 4
• 125000003729 nucleotide group Chemical group 0.000 claims 4
• 230000000903 blocking effect Effects 0.000 claims 3
• 125000003636 chemical group Chemical group 0.000 claims 3
• 229920002477 rna polymer Polymers 0.000 claims 3
• YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
• UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 claims 2
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
• 108020004414 DNA Proteins 0.000 claims 2
• 102000053602 DNA Human genes 0.000 claims 2
• 108091093094 Glycol nucleic acid Proteins 0.000 claims 2
• 101710203389 Outer membrane porin F Proteins 0.000 claims 2
• 108091093037 Peptide nucleic acid Proteins 0.000 claims 2
• 239000002202 Polyethylene glycol Substances 0.000 claims 2
• 108091046915 Threose nucleic acid Proteins 0.000 claims 2
• 150000001413 amino acids Chemical class 0.000 claims 2
• HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims 2
• 238000004132 cross linking Methods 0.000 claims 2
• 230000000694 effects Effects 0.000 claims 2
• 150000007523 nucleic acids Chemical class 0.000 claims 2
• 229920001223 polyethylene glycol Polymers 0.000 claims 2
• 108091005703 transmembrane proteins Proteins 0.000 claims 2
• 102000035160 transmembrane proteins Human genes 0.000 claims 2
• 150000005019 2-aminopurines Chemical class 0.000 claims 1
• HCGYMSSYSAKGPK-UHFFFAOYSA-N 2-nitro-1h-indole Chemical class C1=CC=C2NC([N+](=O)[O-])=CC2=C1 HCGYMSSYSAKGPK-UHFFFAOYSA-N 0.000 claims 1
• OUCMTIKCFRCBHK-UHFFFAOYSA-N 3,3-dibenzylcyclooctyne Chemical group C1CCCCC#CC1(CC=1C=CC=CC=1)CC1=CC=CC=C1 OUCMTIKCFRCBHK-UHFFFAOYSA-N 0.000 claims 1
• NFEXJLMYXXIWPI-JXOAFFINSA-N 5-Hydroxymethylcytidine Chemical class C1=C(CO)C(N)=NC(=O)N1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 NFEXJLMYXXIWPI-JXOAFFINSA-N 0.000 claims 1
• WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical class C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 claims 1
• ZAYHVCMSTBRABG-JXOAFFINSA-N 5-methylcytidine Chemical class O=C1N=C(N)C(C)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](CO)O1 ZAYHVCMSTBRABG-JXOAFFINSA-N 0.000 claims 1
• MSSXOMSJDRHRMC-UHFFFAOYSA-N 9H-purine-2,6-diamine Chemical compound NC1=NC(N)=C2NC=NC2=N1 MSSXOMSJDRHRMC-UHFFFAOYSA-N 0.000 claims 1
• CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 1
• SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 claims 1
• 102100022536 Helicase POLQ-like Human genes 0.000 claims 1
• 108010006464 Hemolysin Proteins Proteins 0.000 claims 1
• 101000899334 Homo sapiens Helicase POLQ-like Proteins 0.000 claims 1
• 102000004895 Lipoproteins Human genes 0.000 claims 1
• 108090001030 Lipoproteins Proteins 0.000 claims 1
• 101710174798 Lysenin Proteins 0.000 claims 1
• 241000187480 Mycobacterium smegmatis Species 0.000 claims 1
• 241000588653 Neisseria Species 0.000 claims 1
• 101710203388 Outer membrane porin G Proteins 0.000 claims 1
• 108010013381 Porins Proteins 0.000 claims 1
• 108010090804 Streptavidin Proteins 0.000 claims 1
• 108010073429 Type V Secretion Systems Proteins 0.000 claims 1
• WREGKURFCTUGRC-POYBYMJQSA-N Zalcitabine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)CC1 WREGKURFCTUGRC-POYBYMJQSA-N 0.000 claims 1
• 150000001251 acridines Chemical class 0.000 claims 1
• 229960002685 biotin Drugs 0.000 claims 1
• 235000020958 biotin Nutrition 0.000 claims 1
• 239000011616 biotin Substances 0.000 claims 1
• 235000012000 cholesterol Nutrition 0.000 claims 1
• 229940107161 cholesterol Drugs 0.000 claims 1
• QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 claims 1
• SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 claims 1
• 230000005669 field effect Effects 0.000 claims 1
• 239000003228 hemolysin Substances 0.000 claims 1
• ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical group CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 claims 1
• 238000002847 impedance measurement Methods 0.000 claims 1
• 239000004615 ingredient Substances 0.000 claims 1
• CXKWCBBOMKCUKX-UHFFFAOYSA-M methylene blue Chemical compound [Cl-].C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 CXKWCBBOMKCUKX-UHFFFAOYSA-M 0.000 claims 1
• 229960000907 methylthioninium chloride Drugs 0.000 claims 1
• 102000039446 nucleic acids Human genes 0.000 claims 1
• 108020004707 nucleic acids Proteins 0.000 claims 1
• 230000003287 optical effect Effects 0.000 claims 1
• 108010014203 outer membrane phospholipase A Proteins 0.000 claims 1
• 229920000642 polymer Polymers 0.000 claims 1
• 229920001184 polypeptide Polymers 0.000 claims 1
• 102000007739 porin activity proteins Human genes 0.000 claims 1
• 108090000765 processed proteins & peptides Proteins 0.000 claims 1
• 102000004196 processed proteins & peptides Human genes 0.000 claims 1
• 102000004169 proteins and genes Human genes 0.000 claims 1
• 108090000623 proteins and genes Proteins 0.000 claims 1
• 150000003839 salts Chemical class 0.000 claims 1
• 239000007787 solid Substances 0.000 claims 1
• 229920001059 synthetic polymer Polymers 0.000 claims 1
• 125000003396 thiol group Chemical class [H]S* 0.000 claims 1