CA2901545C - Use of spacer elements in a nucleic acid to control movement of a helicase - Google Patents
Use of spacer elements in a nucleic acid to control movement of a helicase Download PDFInfo
- Publication number
- CA2901545C CA2901545C CA2901545A CA2901545A CA2901545C CA 2901545 C CA2901545 C CA 2901545C CA 2901545 A CA2901545 A CA 2901545A CA 2901545 A CA2901545 A CA 2901545A CA 2901545 C CA2901545 C CA 2901545C
- Authority
- CA
- Canada
- Prior art keywords
- helicases
- polynucleotide
- spacers
- seq
- target polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Y—ENZYMES
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| PCT/GB2014/050175 WO2014135838A1 (en) | 2013-03-08 | 2014-01-22 | Enzyme stalling method |
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| EP2344891B1 (en) | 2008-09-22 | 2016-03-16 | University Of Washington | Msp nanopores and related methods |
| CN102369298B (zh) | 2009-01-30 | 2017-03-22 | 牛津纳米孔技术有限公司 | 跨膜测序中用于核酸构建体的衔接体 |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| BR112013030529B1 (pt) | 2011-05-27 | 2023-03-07 | Oxford Nanopore Technologies Plc | Método para determinar a presença, ausência ou características de um analito, método e aparelho para sequenciamento de um analito que é um polinucleotídeo alvo, e, kit |
| CN103827320B (zh) | 2011-07-25 | 2017-04-26 | 牛津纳米孔技术有限公司 | 使用跨膜孔进行双链多核苷酸测序的发夹环方法 |
| KR20140090633A (ko) | 2011-10-21 | 2014-07-17 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법 |
| CN104126018B (zh) | 2011-12-29 | 2021-09-14 | 牛津纳米孔技术公司 | 酶方法 |
| CN104220874B (zh) | 2012-02-15 | 2017-05-24 | 牛津纳米孔技术公司 | 适配体方法 |
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