CA2901545C - Use of spacer elements in a nucleic acid to control movement of a helicase - Google Patents
Use of spacer elements in a nucleic acid to control movement of a helicase Download PDFInfo
- Publication number
- CA2901545C CA2901545C CA2901545A CA2901545A CA2901545C CA 2901545 C CA2901545 C CA 2901545C CA 2901545 A CA2901545 A CA 2901545A CA 2901545 A CA2901545 A CA 2901545A CA 2901545 C CA2901545 C CA 2901545C
- Authority
- CA
- Canada
- Prior art keywords
- helicases
- polynucleotide
- spacers
- seq
- target polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/4473—Arrangements for investigating the separated zones, e.g. localising zones by electric means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y306/00—Hydrolases acting on acid anhydrides (3.6)
- C12Y306/04—Hydrolases acting on acid anhydrides (3.6) acting on acid anhydrides; involved in cellular and subcellular movement (3.6.4)
- C12Y306/04012—DNA helicase (3.6.4.12)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Nanotechnology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361774694P | 2013-03-08 | 2013-03-08 | |
| US61/774,694 | 2013-03-08 | ||
| GB1314695.6 | 2013-08-16 | ||
| GBGB1314695.6A GB201314695D0 (en) | 2013-08-16 | 2013-08-16 | Method |
| GB201318464A GB201318464D0 (en) | 2013-10-18 | 2013-10-18 | Modified enzymes |
| GB1318465.0 | 2013-10-18 | ||
| GBGB1318465.0A GB201318465D0 (en) | 2013-10-18 | 2013-10-18 | Method |
| GB1318464.3 | 2013-10-18 | ||
| PCT/GB2014/050175 WO2014135838A1 (en) | 2013-03-08 | 2014-01-22 | Enzyme stalling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2901545A1 CA2901545A1 (en) | 2014-09-12 |
| CA2901545C true CA2901545C (en) | 2019-10-08 |
Family
ID=51490681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2901545A Active CA2901545C (en) | 2013-03-08 | 2014-01-22 | Use of spacer elements in a nucleic acid to control movement of a helicase |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US10221450B2 (enExample) |
| EP (1) | EP2964779B1 (enExample) |
| JP (1) | JP6408494B2 (enExample) |
| KR (1) | KR102168813B1 (enExample) |
| CN (1) | CN105209634B (enExample) |
| AU (1) | AU2014224432B2 (enExample) |
| BR (1) | BR112015021788B1 (enExample) |
| CA (1) | CA2901545C (enExample) |
| SG (1) | SG11201507138RA (enExample) |
| WO (1) | WO2014135838A1 (enExample) |
Families Citing this family (94)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102216783B (zh) | 2008-09-22 | 2015-04-01 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| WO2010086622A1 (en) | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
| EP2633080B1 (en) | 2010-10-29 | 2018-12-05 | President and Fellows of Harvard College | Method of detecting targets using fluorescently labelled nucleic acid nanotube probes |
| EP3633370B1 (en) | 2011-05-27 | 2024-05-01 | Oxford Nanopore Technologies plc | Method and apparatus for determining the presence, absence or characteristics of an analyte |
| IN2014DN00221A (enExample) | 2011-07-25 | 2015-06-05 | Oxford Nanopore Tech Ltd | |
| EP2987870B1 (en) | 2011-10-21 | 2020-02-19 | Oxford Nanopore Technologies Limited | Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor |
| WO2013098562A2 (en) | 2011-12-29 | 2013-07-04 | Oxford Nanopore Technologies Limited | Enzyme method |
| EP2815235B1 (en) | 2012-02-15 | 2020-04-15 | Oxford Nanopore Technologies Limited | Aptamer method |
| JP6271505B2 (ja) | 2012-04-10 | 2018-01-31 | オックスフォード ナノポール テクノロジーズ リミテッド | 変異体ライセニンポア |
| US10808231B2 (en) | 2012-07-19 | 2020-10-20 | Oxford Nanopore Technologies Limited | Modified helicases |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| WO2014041337A1 (en) | 2012-09-14 | 2014-03-20 | Oxford Nanopore Technologies Limited | Sample preparation method |
| WO2014072703A1 (en) | 2012-11-06 | 2014-05-15 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| BR112015021788B1 (pt) | 2013-03-08 | 2023-02-28 | Oxford Nanopore Technologies Plc | Métodos para mover uma ou mais helicases imobilizadas, para controlar o movimento de um polinucleotídeo alvo, para caracterizar um polinucleotídeo alvo e para controlar o carregamento de uma ou mais helicases em um polinucleotídeo alvo, uso de um poro de transmembrana e de um potencial aplicado e de um ou mais espaçadores, complexo, e, kit |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US10378027B2 (en) * | 2013-03-15 | 2019-08-13 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN117947149A (zh) | 2013-10-18 | 2024-04-30 | 牛津纳米孔科技公开有限公司 | 经修饰的酶 |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CA2937411C (en) | 2014-01-22 | 2023-09-26 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| US10294510B2 (en) | 2014-03-11 | 2019-05-21 | President And Fellows Of Harvard College | High-throughput and highly multiplexed imaging with programmable nucleic acid probes |
| EP3126515B1 (en) | 2014-04-04 | 2018-08-29 | Oxford Nanopore Technologies Limited | Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| EP3137627B1 (en) | 2014-05-02 | 2025-07-30 | Oxford Nanopore Technologies plc | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| KR20170042794A (ko) | 2014-09-01 | 2017-04-19 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| CN107109489B (zh) | 2014-10-17 | 2021-11-30 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| EP3974537A1 (en) * | 2015-01-30 | 2022-03-30 | President and Fellows of Harvard College | Microscope-free imaging |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| WO2016166232A1 (en) | 2015-04-14 | 2016-10-20 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| AU2016369071B2 (en) | 2015-12-08 | 2022-05-19 | Katholieke Universiteit Leuven Ku Leuven Research & Development | Modified nanopores, compositions comprising the same, and uses thereof |
| AU2017225374B2 (en) | 2016-03-02 | 2020-03-05 | Oxford Nanopore Technologies Limited | Mutant pore |
| EP4122949B1 (en) | 2016-04-06 | 2024-06-05 | Oxford Nanopore Technologies plc | Mutant pore |
| CN109196116B (zh) | 2016-05-25 | 2022-09-27 | 牛津纳米孔科技公开有限公司 | 一种表征靶多核苷酸的方法 |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| WO2018057502A2 (en) | 2016-09-20 | 2018-03-29 | President And Fellows Harvard College | Molecular verification systems |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| EP3551765B1 (en) | 2016-12-09 | 2026-02-04 | Ultivue, Inc. | Improved methods for multiplex imaging using labeled nucleic acid imaging agents |
| CN108220267B (zh) * | 2016-12-22 | 2022-10-04 | 丰益(上海)生物技术研发中心有限公司 | 磷脂酶及其应用 |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| EP3646025B1 (en) | 2017-06-29 | 2021-08-04 | President and Fellows of Harvard College | Deterministic stepping of polymers through a nanopore |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| AU2019240244A1 (en) | 2018-03-22 | 2020-11-19 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB201808558D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201808554D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907244D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| US20220348606A1 (en) * | 2019-06-26 | 2022-11-03 | Nanjing GenScript Biotech Co., Ltd. | Oligonucleotide containing blocker |
| CN114364797B (zh) * | 2019-09-18 | 2023-10-20 | 株式会社日立高新技术 | 生物分子分析方法 |
| GB201915480D0 (en) | 2019-10-25 | 2019-12-11 | Oxford Nanopore Tech Ltd | Improved nanopore sensing device, components and method of manufacture |
| EP4070092B1 (en) | 2019-12-02 | 2023-08-30 | Oxford Nanopore Technologies PLC | Method of characterising a target polypeptide using a nanopore |
| GB202016874D0 (en) | 2020-10-23 | 2020-12-09 | Oxford Nanopore Tech Ltd | Nanopore support structure and manufacture thereof |
| CN115968410A (zh) * | 2020-06-18 | 2023-04-14 | 牛津纳米孔科技公开有限公司 | 表征移动穿过纳米孔的多核苷酸的方法 |
| CN116075596A (zh) | 2020-08-07 | 2023-05-05 | 牛津纳米孔科技公开有限公司 | 鉴定核酸条形码的方法 |
| CN115777019A (zh) * | 2021-04-06 | 2023-03-10 | 成都齐碳科技有限公司 | 经修饰的Prp43解旋酶及其用途 |
| EP4341433A1 (en) | 2021-05-19 | 2024-03-27 | Oxford Nanopore Technologies PLC | Methods for complement strand sequencing |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| CN113862264A (zh) * | 2021-12-03 | 2021-12-31 | 北京齐碳科技有限公司 | 用于靶多核苷酸测序的衔接子、构建体、方法和应用 |
| CN114262735B (zh) * | 2021-12-15 | 2025-09-16 | 成都齐碳科技有限公司 | 用于表征多核苷酸的衔接体及其用途 |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118906D0 (en) * | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| CN114457145B (zh) * | 2022-01-29 | 2023-08-11 | 成都齐碳科技有限公司 | 用于表征靶多核苷酸测序的接头、构建体、方法和应用 |
| CN120435657A (zh) | 2022-10-28 | 2025-08-05 | 格罗宁根大学 | 蛋白质的基于纳米孔的分析 |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| CN120359304A (zh) | 2022-12-16 | 2025-07-22 | 深圳华大生命科学研究院 | 纳米孔测序方法和试剂盒 |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202307494D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Ltd | Nanopore sequencing and preparation modular instrument |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| GB202407044D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Polymer analysis using transformer neural network |
| GB202407055D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Enhanced polynucleotide sequencing |
| GB202407228D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| GB2644084A (en) | 2024-09-16 | 2026-03-18 | Oxford Nanopore Tech Plc | Augmented consensus and variant calling |
| GB202413618D0 (en) | 2024-09-16 | 2024-10-30 | Oxford Nanopore Tech Plc | peptide characterisation |
| GB202413747D0 (en) | 2024-09-18 | 2024-10-30 | Oxford Nanopore Tech Plc | Preprocessing nanopore signals |
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| WO2016022557A1 (en) | 2014-08-05 | 2016-02-11 | Twist Bioscience Corporation | Cell free cloning of nucleic acids |
| WO2016028887A1 (en) | 2014-08-19 | 2016-02-25 | Pacific Biosciences Of California, Inc. | Compositions and methods for enrichment of nucleic acids |
| US10435685B2 (en) | 2014-08-19 | 2019-10-08 | Pacific Biosciences Of California, Inc. | Compositions and methods for enrichment of nucleic acids |
| KR20170042794A (ko) | 2014-09-01 | 2017-04-19 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| WO2016138080A1 (en) | 2015-02-24 | 2016-09-01 | Trustees Of Boston University | Protection of barcodes during dna amplification using molecular hairpins |
| JP6560336B2 (ja) | 2015-02-26 | 2019-08-14 | 株式会社日立ハイテクノロジーズ | 核酸分子の構築方法 |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| CN107488656B (zh) | 2016-06-13 | 2020-07-17 | 陆欣华 | 一种核酸等温自扩增方法 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
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- 2014-01-22 CA CA2901545A patent/CA2901545C/en active Active
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- 2014-01-22 CN CN201480013152.3A patent/CN105209634B/zh active Active
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| Publication number | Publication date |
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| CN105209634A (zh) | 2015-12-30 |
| US20190211390A1 (en) | 2019-07-11 |
| KR102168813B1 (ko) | 2020-10-22 |
| US20230212665A1 (en) | 2023-07-06 |
| JP6408494B2 (ja) | 2018-10-17 |
| US20160010147A1 (en) | 2016-01-14 |
| AU2014224432A1 (en) | 2015-09-10 |
| KR20150126026A (ko) | 2015-11-10 |
| CN105209634B (zh) | 2020-05-12 |
| BR112015021788B1 (pt) | 2023-02-28 |
| US10221450B2 (en) | 2019-03-05 |
| EP2964779A1 (en) | 2016-01-13 |
| JP2016510593A (ja) | 2016-04-11 |
| WO2014135838A1 (en) | 2014-09-12 |
| CA2901545A1 (en) | 2014-09-12 |
| BR112015021788A8 (pt) | 2022-08-02 |
| AU2014224432B2 (en) | 2019-10-24 |
| BR112015021788A2 (enExample) | 2017-08-29 |
| SG11201507138RA (en) | 2015-10-29 |
| US11560589B2 (en) | 2023-01-24 |
| EP2964779B1 (en) | 2018-08-29 |
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