JP2016500263A5 - - Google Patents
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- Publication number
- JP2016500263A5 JP2016500263A5 JP2015547562A JP2015547562A JP2016500263A5 JP 2016500263 A5 JP2016500263 A5 JP 2016500263A5 JP 2015547562 A JP2015547562 A JP 2015547562A JP 2015547562 A JP2015547562 A JP 2015547562A JP 2016500263 A5 JP2016500263 A5 JP 2016500263A5
- Authority
- JP
- Japan
- Prior art keywords
- bioactive agent
- phosphor
- cell
- bioluminescent reporter
- wavelength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012867 bioactive agent Substances 0.000 claims 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 9
- 230000001413 cellular effect Effects 0.000 claims 5
- 238000000034 method Methods 0.000 claims 3
- 239000000758 substrate Substances 0.000 claims 3
- 238000003556 assay Methods 0.000 claims 2
- 230000004927 fusion Effects 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 238000000862 absorption spectrum Methods 0.000 claims 1
- 238000000225 bioluminescence resonance energy transfer Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000007795 chemical reaction product Substances 0.000 claims 1
- 238000000295 emission spectrum Methods 0.000 claims 1
- 230000005284 excitation Effects 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 150000003384 small molecules Chemical group 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261736429P | 2012-12-12 | 2012-12-12 | |
| US61/736,429 | 2012-12-12 | ||
| US201361794461P | 2013-03-15 | 2013-03-15 | |
| US61/794,461 | 2013-03-15 | ||
| US201361880048P | 2013-09-19 | 2013-09-19 | |
| US61/880,048 | 2013-09-19 | ||
| PCT/US2013/074765 WO2014093677A1 (en) | 2012-12-12 | 2013-12-12 | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019007974A Division JP7092691B2 (ja) | 2012-12-12 | 2019-01-21 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016500263A JP2016500263A (ja) | 2016-01-12 |
| JP2016500263A5 true JP2016500263A5 (enExample) | 2017-02-02 |
| JP6751294B2 JP6751294B2 (ja) | 2020-09-02 |
Family
ID=50934963
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015547562A Active JP6751294B2 (ja) | 2012-12-12 | 2013-12-12 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
| JP2019007974A Active JP7092691B2 (ja) | 2012-12-12 | 2019-01-21 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
| JP2022020320A Active JP7393451B2 (ja) | 2012-12-12 | 2022-02-14 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
| JP2023134744A Active JP7676487B2 (ja) | 2012-12-12 | 2023-08-22 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019007974A Active JP7092691B2 (ja) | 2012-12-12 | 2019-01-21 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
| JP2022020320A Active JP7393451B2 (ja) | 2012-12-12 | 2022-02-14 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
| JP2023134744A Active JP7676487B2 (ja) | 2012-12-12 | 2023-08-22 | 細胞内生体発光共鳴エネルギ移動を用いた生物活性剤による細胞ターゲット結合の認知 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10024862B2 (enExample) |
| EP (2) | EP2932267B1 (enExample) |
| JP (4) | JP6751294B2 (enExample) |
| KR (2) | KR102150479B1 (enExample) |
| CN (2) | CN104871001B (enExample) |
| AU (1) | AU2013359166B2 (enExample) |
| BR (1) | BR112015013710B1 (enExample) |
| CA (2) | CA2894435A1 (enExample) |
| DK (1) | DK2932267T3 (enExample) |
| ES (1) | ES2803503T3 (enExample) |
| PL (1) | PL2932267T3 (enExample) |
| SG (1) | SG11201504525VA (enExample) |
| WO (1) | WO2014093677A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8524457B2 (en) | 2009-09-22 | 2013-09-03 | William Patterson | Method for the selection of specific affinity binders by homogeneous noncompetitive assay |
| CN104871001B (zh) * | 2012-12-12 | 2018-09-11 | 普洛麦格公司 | 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 |
| US10067149B2 (en) * | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| KR20230079494A (ko) | 2013-03-15 | 2023-06-07 | 프로메가 코포레이션 | 구조적 보완에 의한 바이오발광성의 활성화 |
| CN104225611B (zh) * | 2013-06-18 | 2017-06-30 | 天津键凯科技有限公司 | 达沙替尼与非线性构型聚乙二醇的结合物 |
| US11365402B2 (en) | 2014-09-12 | 2022-06-21 | Promega Corporation | Internal protein tags |
| EP3191601B1 (en) | 2014-09-12 | 2022-11-02 | Promega Corporation | Internal protein tags |
| WO2016127100A1 (en) * | 2015-02-05 | 2016-08-11 | Promega Corporation | Luciferase-based thermal shift assays |
| AU2017240233B2 (en) * | 2016-03-31 | 2022-07-14 | University Of Southern California | A highly sensitive and specific luciferase based reporter assay for antigen detection |
| CN109690316A (zh) * | 2016-05-31 | 2019-04-26 | 王帅 | 测定细胞内配体靶蛋白相互作用持久力的方法 |
| EP3535270B3 (en) * | 2016-11-01 | 2024-05-29 | Promega Corporation | Coelenterazine analogues tethered to energy acceptors |
| WO2018139614A1 (ja) * | 2017-01-27 | 2018-08-02 | 国立大学法人大阪大学 | 生体物質の検出方法、それに用いる化学発光指示薬 |
| CN110234996A (zh) * | 2017-02-03 | 2019-09-13 | 国立大学法人大阪大学 | 设备及使用其的判定系统 |
| JP7432529B2 (ja) | 2018-05-30 | 2024-02-16 | プロメガ コーポレイション | 広域スペクトルキナーゼ結合剤 |
| EP3841173A1 (en) | 2018-08-23 | 2021-06-30 | Promega Corporation | Coelenterazine analogues |
| WO2020048607A1 (en) | 2018-09-07 | 2020-03-12 | Université Libre de Bruxelles | Srsf2 as rna epigenetic factor |
| EP3887830A2 (en) | 2018-11-28 | 2021-10-06 | Promega Corporation | Reactive peptide labeling |
| JP7584433B2 (ja) | 2019-03-20 | 2024-11-15 | プロメガ コーポレイション | 光親和性プローブ |
| KR20220007058A (ko) * | 2019-04-10 | 2022-01-18 | 프로메가 코포레이션 | 생물발광을 이용하는 분석물 검출을 위한 조성물 및 방법 |
| CN112147114B (zh) * | 2019-06-27 | 2024-06-25 | 成都先导药物开发股份有限公司 | 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法 |
| WO2021119149A1 (en) | 2019-12-10 | 2021-06-17 | Promega Corporation | Compositions and methods for bioluminescent detection using multifunctional probes |
| JP2023540934A (ja) * | 2020-08-28 | 2023-09-27 | プロメガ コーポレイション | Ｒａｓタンパク質のターゲットエンゲージメントアッセイ |
| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
| CN114394970B (zh) * | 2022-01-05 | 2023-06-06 | 山东大学 | 一种酯类Furimazine衍生物及制备方法与应用 |
| CN114813706B (zh) * | 2022-06-29 | 2022-12-13 | 国科大杭州高等研究院 | 一种血细胞高光谱光镊捕获能量共振转移分析仪 |
| CN120051474A (zh) | 2022-10-14 | 2025-05-27 | 普罗美加公司 | 腔肠素类似物 |
| CN116354927B (zh) * | 2023-02-21 | 2025-02-07 | 杭州医学院 | 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用 |
| WO2024229422A2 (en) * | 2023-05-04 | 2024-11-07 | Promega Corporation | Complementation-based tags and reporters for dual-modality labeling |
| KR20250004513A (ko) | 2023-06-29 | 2025-01-08 | 부산대학교 산학협력단 | Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주 |
| CN117330755B (zh) * | 2023-08-25 | 2024-03-26 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
| KR102802452B1 (ko) | 2023-11-07 | 2025-05-08 | 대한민국 | LgBiT 단백질을 발현하는 세포주 및 HiBiT 단백질을 발현하는 구제역 바이러스를 이용한 구제역 바이러스 감염 여부 확인방법 |
| US20250206747A1 (en) | 2023-12-20 | 2025-06-26 | Promega Corporation | Coelenterazine analogues |
| GB202410534D0 (en) | 2024-07-18 | 2024-09-04 | Nrg Therapeutics Ltd | Novel method and uses |
| CN119709661B (zh) * | 2024-12-30 | 2025-10-10 | 佰翱得(无锡)新药开发有限公司 | 一种荧光素酶OLuc突变体及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5928888A (en) * | 1996-09-26 | 1999-07-27 | Aurora Biosciences Corporation | Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities |
| CA2335305C (en) | 1998-06-16 | 2006-05-23 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) system and its use |
| US20030203404A1 (en) | 1999-12-22 | 2003-10-30 | Erik Joly | Bioluminescence resonance energy transfer( bret) system with broad spectral resolution between donor and acceptor emission wavelengths and its use |
| EP2085781B2 (en) | 2000-10-06 | 2020-03-11 | Life Technologies Corporation | Transfectable micelles containing semiconductor nanocrystals |
| DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
| US20060099646A1 (en) | 2002-10-11 | 2006-05-11 | Anders Heding | Bret assay |
| GB0307559D0 (en) | 2003-04-02 | 2003-05-07 | Univ Nottingham | Fluorescently tagged ligands |
| CN100473988C (zh) * | 2004-07-08 | 2009-04-01 | 厦门大学 | 具有荧光共振能量转移特点的蛋白组合及其用途 |
| US9029097B2 (en) | 2005-02-16 | 2015-05-12 | Universite De Montreal | Biosensors for monitoring receptor-mediated G-protein activation |
| GB0625678D0 (en) * | 2006-12-21 | 2007-01-31 | Lux Biotechnology Ltd | Composition and method for detection of demineralisation |
| US20080299592A1 (en) * | 2007-03-02 | 2008-12-04 | Miller Stephen C | Red-Shifted Luciferase |
| EP2006686A1 (en) * | 2007-06-22 | 2008-12-24 | Institut Pasteur | Method for screening for selective modulator of the NF-kB pathway activation |
| WO2009061413A2 (en) * | 2007-11-05 | 2009-05-14 | Promega Corporation | Hybrid fusion reporter and uses thereof |
| US8962341B2 (en) * | 2008-12-12 | 2015-02-24 | The Commonwealth Medical College | Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis |
| PL3744834T3 (pl) | 2009-05-01 | 2026-01-05 | Promega Corporation | Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło |
| US8263350B2 (en) * | 2009-06-29 | 2012-09-11 | The University Of Chicago | Molecular affinity clamp technology and uses thereof |
| EP2635582B1 (en) | 2010-11-02 | 2016-01-27 | Promega Corporation | Novel coelenterazine substrates and methods of use |
| WO2013078244A1 (en) * | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
| CN102798717B (zh) * | 2012-06-15 | 2014-11-26 | 杭州师范大学 | 一种o6-甲基鸟嘌呤-dna甲基转移酶活性检测方法 |
| US10067149B2 (en) | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| CN104871001B (zh) | 2012-12-12 | 2018-09-11 | 普洛麦格公司 | 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 |
-
2013
- 2013-12-12 CN CN201380064252.4A patent/CN104871001B/zh active Active
- 2013-12-12 EP EP13861977.0A patent/EP2932267B1/en active Active
- 2013-12-12 WO PCT/US2013/074765 patent/WO2014093677A1/en not_active Ceased
- 2013-12-12 CA CA2894435A patent/CA2894435A1/en active Pending
- 2013-12-12 JP JP2015547562A patent/JP6751294B2/ja active Active
- 2013-12-12 ES ES13861977T patent/ES2803503T3/es active Active
- 2013-12-12 KR KR1020157018230A patent/KR102150479B1/ko active Active
- 2013-12-12 PL PL13861977T patent/PL2932267T3/pl unknown
- 2013-12-12 EP EP20169678.8A patent/EP3712154A1/en active Pending
- 2013-12-12 SG SG11201504525VA patent/SG11201504525VA/en unknown
- 2013-12-12 KR KR1020207024622A patent/KR102292431B1/ko active Active
- 2013-12-12 US US14/104,860 patent/US10024862B2/en active Active
- 2013-12-12 AU AU2013359166A patent/AU2013359166B2/en active Active
- 2013-12-12 DK DK13861977.0T patent/DK2932267T3/da active
- 2013-12-12 BR BR112015013710-5A patent/BR112015013710B1/pt active IP Right Grant
- 2013-12-12 CN CN201810902469.2A patent/CN109612981B/zh active Active
- 2013-12-12 CA CA3174484A patent/CA3174484A1/en active Pending
-
2019
- 2019-01-21 JP JP2019007974A patent/JP7092691B2/ja active Active
-
2022
- 2022-02-14 JP JP2022020320A patent/JP7393451B2/ja active Active
-
2023
- 2023-08-22 JP JP2023134744A patent/JP7676487B2/ja active Active
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