CA2894435A1 - Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer - Google Patents

Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer Download PDF

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Publication number
CA2894435A1
CA2894435A1 CA2894435A CA2894435A CA2894435A1 CA 2894435 A1 CA2894435 A1 CA 2894435A1 CA 2894435 A CA2894435 A CA 2894435A CA 2894435 A CA2894435 A CA 2894435A CA 2894435 A1 CA2894435 A1 CA 2894435A1
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CA
Canada
Prior art keywords
bioactive agent
bret
fluorophore
nanoluc
target
Prior art date
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Pending
Application number
CA2894435A
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English (en)
French (fr)
Inventor
Carolyn W. HITKO
Thomas Kirkland
Thomas Machleidt
Rachel F. OHANA
Matt ROBERS
Keith Wood
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Promega Corp
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Promega Corp
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Application filed by Promega Corp filed Critical Promega Corp
Priority to CA3174484A priority Critical patent/CA3174484A1/en
Publication of CA2894435A1 publication Critical patent/CA2894435A1/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/10Libraries containing peptides or polypeptides, or derivatives thereof
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2894435A 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer Pending CA2894435A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3174484A CA3174484A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3174484A Division CA3174484A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Publications (1)

Publication Number Publication Date
CA2894435A1 true CA2894435A1 (en) 2014-06-19

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CA2894435A Pending CA2894435A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
CA3174484A Pending CA3174484A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

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US (1) US10024862B2 (enExample)
EP (2) EP2932267B1 (enExample)
JP (4) JP6751294B2 (enExample)
KR (2) KR102150479B1 (enExample)
CN (2) CN104871001B (enExample)
AU (1) AU2013359166B2 (enExample)
BR (1) BR112015013710B1 (enExample)
CA (2) CA2894435A1 (enExample)
DK (1) DK2932267T3 (enExample)
ES (1) ES2803503T3 (enExample)
PL (1) PL2932267T3 (enExample)
SG (1) SG11201504525VA (enExample)
WO (1) WO2014093677A1 (enExample)

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CA2894435A1 (en) 2012-12-12 2014-06-19 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
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EP3535270B3 (en) * 2016-11-01 2024-05-29 Promega Corporation Coelenterazine analogues tethered to energy acceptors
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JP7137842B2 (ja) * 2017-02-03 2022-09-15 国立大学法人大阪大学 デバイス、及びそれを用いた判定システム
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CN112513188B (zh) 2018-08-23 2024-01-26 普洛麦格公司 腔肠素类似物
WO2020048607A1 (en) 2018-09-07 2020-03-12 Université Libre de Bruxelles Srsf2 as rna epigenetic factor
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WO2020210658A1 (en) * 2019-04-10 2020-10-15 Promega Corporation Compositions and methods for analyte detection using bioluminescence
CN112147114B (zh) * 2019-06-27 2024-06-25 成都先导药物开发股份有限公司 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法
JP7745550B2 (ja) 2019-12-10 2025-09-29 プロメガ コーポレイション 多官能性プローブを使用する生物発光検出用組成物及び生物発光検出方法
JP2023540934A (ja) * 2020-08-28 2023-09-27 プロメガ コーポレイション Rasタンパク質のターゲットエンゲージメントアッセイ
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN114394970B (zh) * 2022-01-05 2023-06-06 山东大学 一种酯类Furimazine衍生物及制备方法与应用
CN114813706B (zh) * 2022-06-29 2022-12-13 国科大杭州高等研究院 一种血细胞高光谱光镊捕获能量共振转移分析仪
JP2025536247A (ja) 2022-10-14 2025-11-05 プロメガ コーポレイション セレンテラジン類似体
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
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Also Published As

Publication number Publication date
AU2013359166A1 (en) 2015-07-09
JP2016500263A (ja) 2016-01-12
PL2932267T3 (pl) 2020-10-19
JP7676487B2 (ja) 2025-05-14
AU2013359166B2 (en) 2019-09-19
EP2932267B1 (en) 2020-05-06
EP2932267A4 (en) 2016-06-22
EP3712154A1 (en) 2020-09-23
JP6751294B2 (ja) 2020-09-02
US10024862B2 (en) 2018-07-17
US20140194307A1 (en) 2014-07-10
JP7092691B2 (ja) 2022-06-28
CA3174484A1 (en) 2014-06-19
KR20150094696A (ko) 2015-08-19
JP2023179413A (ja) 2023-12-19
CN109612981B (zh) 2022-05-13
KR102150479B1 (ko) 2020-09-01
BR112015013710B1 (pt) 2022-08-09
EP2932267A1 (en) 2015-10-21
KR102292431B1 (ko) 2021-08-20
CN104871001B (zh) 2018-09-11
JP7393451B2 (ja) 2023-12-06
CN104871001A (zh) 2015-08-26
JP2022078077A (ja) 2022-05-24
BR112015013710A2 (pt) 2018-11-21
KR20200104928A (ko) 2020-09-04
JP2019071899A (ja) 2019-05-16
CN109612981A (zh) 2019-04-12
ES2803503T3 (es) 2021-01-27
DK2932267T3 (da) 2020-07-20
WO2014093677A1 (en) 2014-06-19
SG11201504525VA (en) 2015-07-30

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