CN104871001B - 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 - Google Patents

使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 Download PDF

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CN104871001B
CN104871001B CN201380064252.4A CN201380064252A CN104871001B CN 104871001 B CN104871001 B CN 104871001B CN 201380064252 A CN201380064252 A CN 201380064252A CN 104871001 B CN104871001 B CN 104871001B
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bret
nanoluc
target
binding
bioactive agent
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CN104871001A (zh
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C·W·希特科
T·柯克兰
T·马赫莱特
R·F·奥哈纳
M·罗贝斯
K·伍德
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Promega Corp
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CN201380064252.4A 2012-12-12 2013-12-12 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 Active CN104871001B (zh)

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US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

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CN114813706B (zh) * 2022-06-29 2022-12-13 国科大杭州高等研究院 一种血细胞高光谱光镊捕获能量共振转移分析仪
JP2025536247A (ja) 2022-10-14 2025-11-05 プロメガ コーポレイション セレンテラジン類似体
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
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CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
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KR102292431B1 (ko) 2021-08-20
JP2022078077A (ja) 2022-05-24
EP2932267A4 (en) 2016-06-22
CA3174484A1 (en) 2014-06-19
ES2803503T3 (es) 2021-01-27
JP7676487B2 (ja) 2025-05-14
JP2016500263A (ja) 2016-01-12
US20140194307A1 (en) 2014-07-10
EP2932267A1 (en) 2015-10-21
CN109612981A (zh) 2019-04-12
BR112015013710B1 (pt) 2022-08-09
AU2013359166A1 (en) 2015-07-09
JP7092691B2 (ja) 2022-06-28
PL2932267T3 (pl) 2020-10-19
WO2014093677A1 (en) 2014-06-19
JP6751294B2 (ja) 2020-09-02
KR20150094696A (ko) 2015-08-19
JP2023179413A (ja) 2023-12-19
KR102150479B1 (ko) 2020-09-01
AU2013359166B2 (en) 2019-09-19
JP7393451B2 (ja) 2023-12-06
BR112015013710A2 (pt) 2018-11-21
CA2894435A1 (en) 2014-06-19
JP2019071899A (ja) 2019-05-16
SG11201504525VA (en) 2015-07-30
CN104871001A (zh) 2015-08-26
EP2932267B1 (en) 2020-05-06
US10024862B2 (en) 2018-07-17
CN109612981B (zh) 2022-05-13
KR20200104928A (ko) 2020-09-04
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