KR102292431B1 - 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 - Google Patents

세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Download PDF

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KR102292431B1
KR102292431B1 KR1020207024622A KR20207024622A KR102292431B1 KR 102292431 B1 KR102292431 B1 KR 102292431B1 KR 1020207024622 A KR1020207024622 A KR 1020207024622A KR 20207024622 A KR20207024622 A KR 20207024622A KR 102292431 B1 KR102292431 B1 KR 102292431B1
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bioactive agent
fluorophore
leu
gly
bret
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캐롤린 더블유. 히트코
토마스 커클랜드
토마스 마흐라이트
레이첼 에프. 오하나
매트 로버스
키이스 우드
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프로메가 코포레이션
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
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    • C07—ORGANIC CHEMISTRY
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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KR1020207024622A 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식 Active KR102292431B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
KR1020157018230A KR102150479B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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KR1020157018230A Active KR102150479B1 (ko) 2012-12-12 2013-12-12 세포내 생물발광 공명 에너지 전이를 이용하여 생물활성제에 의한 세포 표적 결합의 인식

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CN (2) CN104871001B (enExample)
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BR (1) BR112015013710B1 (enExample)
CA (2) CA3174484A1 (enExample)
DK (1) DK2932267T3 (enExample)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8524457B2 (en) 2009-09-22 2013-09-03 William Patterson Method for the selection of specific affinity binders by homogeneous noncompetitive assay
BR112015013710B1 (pt) * 2012-12-12 2022-08-09 Promega Corporation Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular
US10067149B2 (en) * 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
AU2014236949C1 (en) 2013-03-15 2018-11-29 Promega Corporation Activation of bioluminescence by structural complementation
CN104225611B (zh) * 2013-06-18 2017-06-30 天津键凯科技有限公司 达沙替尼与非线性构型聚乙二醇的结合物
EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3254113B1 (en) 2015-02-05 2024-10-09 Promega Corporation Luciferase-based thermal shift assays
CA3018253A1 (en) * 2016-03-31 2017-10-05 University Of Southern California A highly sensitive and specific luciferase based reporter assay for antigen detection
CN109690316A (zh) * 2016-05-31 2019-04-26 王帅 测定细胞内配体靶蛋白相互作用持久力的方法
EP4144738A1 (en) * 2016-11-01 2023-03-08 Promega Corporation Coelenterazine analogues tethered to energy acceptors
JP7014437B2 (ja) * 2017-01-27 2022-02-01 国立大学法人大阪大学 生体物質の検出方法、それに用いる化学発光指示薬
US11619628B2 (en) * 2017-02-03 2023-04-04 Osaka University Device and determination system using same
EP3803401A4 (en) 2018-05-30 2022-02-23 Promega Corporation BROAD SPECTRUM KINASE BINDERS
CN112513188B (zh) 2018-08-23 2024-01-26 普洛麦格公司 腔肠素类似物
WO2020048607A1 (en) 2018-09-07 2020-03-12 Université Libre de Bruxelles Srsf2 as rna epigenetic factor
US11175228B2 (en) 2018-11-28 2021-11-16 Promega Corporation Reactive peptide labeling
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
EP3953374A4 (en) * 2019-04-10 2023-06-07 Promega Corporation COMPOSITIONS AND METHODS FOR DETECTION OF ANALYTES USING BIOLUMINESCENCE
CN112147114B (zh) * 2019-06-27 2024-06-25 成都先导药物开发股份有限公司 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法
JP7745550B2 (ja) 2019-12-10 2025-09-29 プロメガ コーポレイション 多官能性プローブを使用する生物発光検出用組成物及び生物発光検出方法
WO2022047186A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
CN115161296B (zh) * 2021-04-01 2024-08-13 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN114394970B (zh) * 2022-01-05 2023-06-06 山东大学 一种酯类Furimazine衍生物及制备方法与应用
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US20240150363A1 (en) 2022-10-14 2024-05-09 Promega Corporation Coelenterazine analogues
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
AU2024265176A1 (en) * 2023-05-04 2025-12-11 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
KR102802452B1 (ko) 2023-11-07 2025-05-08 대한민국 LgBiT 단백질을 발현하는 세포주 및 HiBiT 단백질을 발현하는 구제역 바이러스를 이용한 구제역 바이러스 감염 여부 확인방법
US20250206747A1 (en) 2023-12-20 2025-06-26 Promega Corporation Coelenterazine analogues
GB202410534D0 (en) 2024-07-18 2024-09-04 Nrg Therapeutics Ltd Novel method and uses
CN119709661B (zh) * 2024-12-30 2025-10-10 佰翱得(无锡)新药开发有限公司 一种荧光素酶OLuc突变体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004034054A2 (en) * 2002-10-11 2004-04-22 7Tm Pharma A/S Method for enhancing and prolonging the bioluminescence resonance energy transfer (bret) signal in a bret assay and a substrate solution for use in a bret assay
US20080299592A1 (en) 2007-03-02 2008-12-04 Miller Stephen C Red-Shifted Luciferase

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
AU4254299A (en) 1998-06-16 2000-01-05 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system and its use
EP1295121B1 (en) 1999-12-22 2006-03-08 PerkinElmer BioSignal Inc. A bioluminescence resonance energy transfer (bret) system with broad spectral resolution between donor and acceptor emission wavelengths and its use
EP2085781B2 (en) 2000-10-06 2020-03-11 Life Technologies Corporation Transfectable micelles containing semiconductor nanocrystals
DE10058091A1 (de) * 2000-11-23 2002-06-06 Bayer Ag Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung
GB0307559D0 (en) * 2003-04-02 2003-05-07 Univ Nottingham Fluorescently tagged ligands
CN100473988C (zh) * 2004-07-08 2009-04-01 厦门大学 具有荧光共振能量转移特点的蛋白组合及其用途
US9029097B2 (en) * 2005-02-16 2015-05-12 Universite De Montreal Biosensors for monitoring receptor-mediated G-protein activation
GB0625678D0 (en) * 2006-12-21 2007-01-31 Lux Biotechnology Ltd Composition and method for detection of demineralisation
EP2006686A1 (en) * 2007-06-22 2008-12-24 Institut Pasteur Method for screening for selective modulator of the NF-kB pathway activation
US20090253131A1 (en) * 2007-11-05 2009-10-08 Promega Corporation Hybrid fusion reporter and uses thereof
CA2747244A1 (en) * 2008-12-12 2010-06-17 University Of Florida Research Foundation, Inc. Cell-based detection of apf through its interaction with ckap4 for diagnosis of interstitial cystitis
WO2010127368A1 (en) 2009-05-01 2010-11-04 Promega Corporation Synthetic oplophorus luciferases with enhanced light output
US8263350B2 (en) * 2009-06-29 2012-09-11 The University Of Chicago Molecular affinity clamp technology and uses thereof
SG10202103336SA (en) * 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
EP2782916B1 (en) * 2011-11-21 2018-02-28 Promega Corporation Carboxy x rhodamine analogs
CN102798717B (zh) * 2012-06-15 2014-11-26 杭州师范大学 一种o6-甲基鸟嘌呤-dna甲基转移酶活性检测方法
US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
BR112015013710B1 (pt) 2012-12-12 2022-08-09 Promega Corporation Métodos in vitro para detectar uma interação entre um agente bioativo e um alvo celular

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004034054A2 (en) * 2002-10-11 2004-04-22 7Tm Pharma A/S Method for enhancing and prolonging the bioluminescence resonance energy transfer (bret) signal in a bret assay and a substrate solution for use in a bret assay
US20080299592A1 (en) 2007-03-02 2008-12-04 Miller Stephen C Red-Shifted Luciferase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Mary P. Hall et al., 'Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate', ACS Chem. Biol., 2012, Vol. 7, pp 1848-1857. 1부.*
Yan Zhang et al., 'HaloTag Protein-Mediated Site-Specific Conjugation of Bioluminescent Proteins to Quantum Dots', Angew. Chem. Int. Ed., 2006, Vol 45, pp 4936-4940. 1부.*

Cited By (1)

* Cited by examiner, † Cited by third party
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KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주

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US10024862B2 (en) 2018-07-17
PL2932267T3 (pl) 2020-10-19
JP2019071899A (ja) 2019-05-16
SG11201504525VA (en) 2015-07-30
KR20200104928A (ko) 2020-09-04
BR112015013710B1 (pt) 2022-08-09
KR20150094696A (ko) 2015-08-19
AU2013359166A1 (en) 2015-07-09
EP3712154A1 (en) 2020-09-23
EP2932267B1 (en) 2020-05-06
KR102150479B1 (ko) 2020-09-01
WO2014093677A1 (en) 2014-06-19
JP7092691B2 (ja) 2022-06-28
EP2932267A1 (en) 2015-10-21
US20140194307A1 (en) 2014-07-10
JP7393451B2 (ja) 2023-12-06
DK2932267T3 (da) 2020-07-20
ES2803503T3 (es) 2021-01-27
AU2013359166B2 (en) 2019-09-19
CN104871001A (zh) 2015-08-26
BR112015013710A2 (pt) 2018-11-21
JP2016500263A (ja) 2016-01-12
JP2023179413A (ja) 2023-12-19
CA3174484A1 (en) 2014-06-19
CN104871001B (zh) 2018-09-11
EP2932267A4 (en) 2016-06-22
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