CA3174484A1 - Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer - Google Patents

Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Info

Publication number
CA3174484A1
CA3174484A1 CA3174484A CA3174484A CA3174484A1 CA 3174484 A1 CA3174484 A1 CA 3174484A1 CA 3174484 A CA3174484 A CA 3174484A CA 3174484 A CA3174484 A CA 3174484A CA 3174484 A1 CA3174484 A1 CA 3174484A1
Authority
CA
Canada
Prior art keywords
bioactive agent
bret
binding
nanoluc
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3174484A
Other languages
English (en)
French (fr)
Inventor
Carolyn W. HITKO
Thomas Kirkland
Thomas Machleidt
Rachel F. OHANA
Matt ROBERS
Keith Wood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of CA3174484A1 publication Critical patent/CA3174484A1/en
Pending legal-status Critical Current

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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/10Libraries containing peptides or polypeptides, or derivatives thereof
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA3174484A 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer Pending CA3174484A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
CA2894435A CA2894435A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2894435A Division CA2894435A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Publications (1)

Publication Number Publication Date
CA3174484A1 true CA3174484A1 (en) 2014-06-19

Family

ID=50934963

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3174484A Pending CA3174484A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
CA2894435A Pending CA2894435A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2894435A Pending CA2894435A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Country Status (13)

Country Link
US (1) US10024862B2 (enExample)
EP (2) EP3712154A1 (enExample)
JP (4) JP6751294B2 (enExample)
KR (2) KR102150479B1 (enExample)
CN (2) CN104871001B (enExample)
AU (1) AU2013359166B2 (enExample)
BR (1) BR112015013710B1 (enExample)
CA (2) CA3174484A1 (enExample)
DK (1) DK2932267T3 (enExample)
ES (1) ES2803503T3 (enExample)
PL (1) PL2932267T3 (enExample)
SG (1) SG11201504525VA (enExample)
WO (1) WO2014093677A1 (enExample)

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US10067149B2 (en) 2012-12-12 2018-09-04 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
US10024862B2 (en) * 2012-12-12 2018-07-17 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
ES2926463T3 (es) * 2013-03-15 2022-10-26 Promega Corp Activación de la bioluminiscencia mediante complementación estructural
CN104225611B (zh) * 2013-06-18 2017-06-30 天津键凯科技有限公司 达沙替尼与非线性构型聚乙二醇的结合物
US11365402B2 (en) 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP3191601B1 (en) * 2014-09-12 2022-11-02 Promega Corporation Internal protein tags
WO2016127100A1 (en) * 2015-02-05 2016-08-11 Promega Corporation Luciferase-based thermal shift assays
EP3436037A4 (en) * 2016-03-31 2019-12-04 University of Southern California HIGHLY SENSITIVE AND SPECIFIC LUCIFERASE-BASED REPORTER TEST FOR ANTIGEN DETECTION
WO2017206041A1 (zh) * 2016-05-31 2017-12-07 王帅 测定细胞内配体靶蛋白相互作用持久力的方法
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CN110226087A (zh) * 2017-01-27 2019-09-10 国立大学法人大阪大学 生物体物质的检测方法、该方法中使用的化学发光指示剂
EP3578978B1 (en) * 2017-02-03 2022-07-13 Osaka University Device and determination system using same
US11442063B2 (en) 2018-05-30 2022-09-13 Promega Corporation Broad spectrum kinase binding agents
WO2020041579A1 (en) 2018-08-23 2020-02-27 Promega Corporation Coelenterazine analogues
WO2020048607A1 (en) 2018-09-07 2020-03-12 Université Libre de Bruxelles Srsf2 as rna epigenetic factor
JP7703446B2 (ja) 2018-11-28 2025-07-07 プロメガ コーポレイション 反応性ペプチド標識付け
JP7584433B2 (ja) 2019-03-20 2024-11-15 プロメガ コーポレイション 光親和性プローブ
EP3953374A4 (en) * 2019-04-10 2023-06-07 Promega Corporation COMPOSITIONS AND METHODS FOR DETECTION OF ANALYTES USING BIOLUMINESCENCE
CN112147114B (zh) * 2019-06-27 2024-06-25 成都先导药物开发股份有限公司 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法
EP4073512B1 (en) 2019-12-10 2025-11-12 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
US20220064696A1 (en) * 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
CN118703457A (zh) * 2021-04-01 2024-09-27 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
CN114394970B (zh) * 2022-01-05 2023-06-06 山东大学 一种酯类Furimazine衍生物及制备方法与应用
CN114813706B (zh) * 2022-06-29 2022-12-13 国科大杭州高等研究院 一种血细胞高光谱光镊捕获能量共振转移分析仪
JP2025536247A (ja) 2022-10-14 2025-11-05 プロメガ コーポレイション セレンテラジン類似体
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
US20240368565A1 (en) * 2023-05-04 2024-11-07 Promega Corporation Complementation-based tags and reporters for dual-modality labeling
KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
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Also Published As

Publication number Publication date
KR102292431B1 (ko) 2021-08-20
JP2022078077A (ja) 2022-05-24
EP2932267A4 (en) 2016-06-22
ES2803503T3 (es) 2021-01-27
JP7676487B2 (ja) 2025-05-14
JP2016500263A (ja) 2016-01-12
US20140194307A1 (en) 2014-07-10
EP2932267A1 (en) 2015-10-21
CN109612981A (zh) 2019-04-12
BR112015013710B1 (pt) 2022-08-09
AU2013359166A1 (en) 2015-07-09
JP7092691B2 (ja) 2022-06-28
PL2932267T3 (pl) 2020-10-19
WO2014093677A1 (en) 2014-06-19
JP6751294B2 (ja) 2020-09-02
KR20150094696A (ko) 2015-08-19
JP2023179413A (ja) 2023-12-19
KR102150479B1 (ko) 2020-09-01
AU2013359166B2 (en) 2019-09-19
JP7393451B2 (ja) 2023-12-06
CN104871001B (zh) 2018-09-11
BR112015013710A2 (pt) 2018-11-21
CA2894435A1 (en) 2014-06-19
JP2019071899A (ja) 2019-05-16
SG11201504525VA (en) 2015-07-30
CN104871001A (zh) 2015-08-26
EP2932267B1 (en) 2020-05-06
US10024862B2 (en) 2018-07-17
CN109612981B (zh) 2022-05-13
KR20200104928A (ko) 2020-09-04
EP3712154A1 (en) 2020-09-23
DK2932267T3 (da) 2020-07-20

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