AU2013359166B2 - Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer - Google Patents

Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer Download PDF

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AU2013359166B2
AU2013359166B2 AU2013359166A AU2013359166A AU2013359166B2 AU 2013359166 B2 AU2013359166 B2 AU 2013359166B2 AU 2013359166 A AU2013359166 A AU 2013359166A AU 2013359166 A AU2013359166 A AU 2013359166A AU 2013359166 B2 AU2013359166 B2 AU 2013359166B2
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bioactive agent
bret
target
binding
nanoluc
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AU2013359166A1 (en
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Carolyn W. HITKO
Thomas Kirkland
Thomas Machleidt
Rachel F. OHANA
Matt ROBERS
Keith Wood
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Promega Corp
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Promega Corp
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AU2013359166A 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer Active AU2013359166B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261736429P 2012-12-12 2012-12-12
US61/736,429 2012-12-12
US201361794461P 2013-03-15 2013-03-15
US61/794,461 2013-03-15
US201361880048P 2013-09-19 2013-09-19
US61/880,048 2013-09-19
PCT/US2013/074765 WO2014093677A1 (en) 2012-12-12 2013-12-12 Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer

Publications (2)

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AU2013359166A1 AU2013359166A1 (en) 2015-07-09
AU2013359166B2 true AU2013359166B2 (en) 2019-09-19

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EP (2) EP2932267B1 (enExample)
JP (4) JP6751294B2 (enExample)
KR (2) KR102150479B1 (enExample)
CN (2) CN109612981B (enExample)
AU (1) AU2013359166B2 (enExample)
BR (1) BR112015013710B1 (enExample)
CA (2) CA2894435A1 (enExample)
DK (1) DK2932267T3 (enExample)
ES (1) ES2803503T3 (enExample)
PL (1) PL2932267T3 (enExample)
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US10024862B2 (en) * 2012-12-12 2018-07-17 Promega Corporation Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer
AU2014236949C1 (en) 2013-03-15 2018-11-29 Promega Corporation Activation of bioluminescence by structural complementation
CN104225611B (zh) * 2013-06-18 2017-06-30 天津键凯科技有限公司 达沙替尼与非线性构型聚乙二醇的结合物
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EP4219736A1 (en) * 2014-09-12 2023-08-02 Promega Corporation Internal protein tags
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CN109690316A (zh) * 2016-05-31 2019-04-26 王帅 测定细胞内配体靶蛋白相互作用持久力的方法
JP7200102B2 (ja) * 2016-11-01 2023-01-06 プロメガ コーポレイション エネルギー受容体に係留されたセレンテラジン類縁体
EP3575779A1 (en) * 2017-01-27 2019-12-04 Osaka University Method of detecting biological material, and chemiluminescent indicator used therein
EP3578978B1 (en) * 2017-02-03 2022-07-13 Osaka University Device and determination system using same
WO2019232225A1 (en) 2018-05-30 2019-12-05 Promega Corporation Broad-spectrum kinase binding agents
JP7653351B2 (ja) 2018-08-23 2025-03-28 プロメガ コーポレイション セレンテラジン類似体
WO2020048607A1 (en) 2018-09-07 2020-03-12 Université Libre de Bruxelles Srsf2 as rna epigenetic factor
WO2020113036A2 (en) 2018-11-28 2020-06-04 Promega Corporation Reactive peptide labeling
US11913944B2 (en) 2019-03-20 2024-02-27 Promega Corporation Photoaffinity probes
AU2020272037B2 (en) * 2019-04-10 2025-11-27 Promega Corporation Compositions and methods for analyte detection using bioluminescence
CN112147114B (zh) * 2019-06-27 2024-06-25 成都先导药物开发股份有限公司 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法
WO2021119149A1 (en) 2019-12-10 2021-06-17 Promega Corporation Compositions and methods for bioluminescent detection using multifunctional probes
WO2022047186A1 (en) 2020-08-28 2022-03-03 Promega Corporation Target engagement assay for ras proteins
CN115161296B (zh) * 2021-04-01 2024-08-13 深圳华大生命科学研究院 一种刺虾荧光素酶Nluc的突变体及其应用
EP4337646A4 (en) 2021-05-13 2025-05-21 Promega Corporation BIOLUMINESCENT DETECTION OF DNA SYNTHESIS
CN114394970B (zh) * 2022-01-05 2023-06-06 山东大学 一种酯类Furimazine衍生物及制备方法与应用
CN114813706B (zh) * 2022-06-29 2022-12-13 国科大杭州高等研究院 一种血细胞高光谱光镊捕获能量共振转移分析仪
CN120051474A (zh) 2022-10-14 2025-05-27 普罗美加公司 腔肠素类似物
CN116354927B (zh) * 2023-02-21 2025-02-07 杭州医学院 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用
CN121794397A (zh) * 2023-05-04 2026-04-03 普洛麦格公司 用于双模态标记的基于互补性的标签和报告基因
KR20250004513A (ko) 2023-06-29 2025-01-08 부산대학교 산학협력단 Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주
CN117330755B (zh) * 2023-08-25 2024-03-26 广东省农业科学院农业生物基因研究中心 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法
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CA2894435A1 (en) 2014-06-19
KR102150479B1 (ko) 2020-09-01
CA3174484A1 (en) 2014-06-19
JP7393451B2 (ja) 2023-12-06
JP7092691B2 (ja) 2022-06-28
KR102292431B1 (ko) 2021-08-20
US20140194307A1 (en) 2014-07-10
JP6751294B2 (ja) 2020-09-02
PL2932267T3 (pl) 2020-10-19
JP7676487B2 (ja) 2025-05-14
KR20150094696A (ko) 2015-08-19
JP2016500263A (ja) 2016-01-12
KR20200104928A (ko) 2020-09-04
WO2014093677A1 (en) 2014-06-19
EP3712154A1 (en) 2020-09-23
BR112015013710B1 (pt) 2022-08-09
CN104871001A (zh) 2015-08-26
EP2932267A1 (en) 2015-10-21
SG11201504525VA (en) 2015-07-30
JP2023179413A (ja) 2023-12-19
DK2932267T3 (da) 2020-07-20
US10024862B2 (en) 2018-07-17
EP2932267A4 (en) 2016-06-22
CN109612981A (zh) 2019-04-12
CA3174484C (en) 2026-06-09
CN109612981B (zh) 2022-05-13
AU2013359166A1 (en) 2015-07-09
JP2019071899A (ja) 2019-05-16
ES2803503T3 (es) 2021-01-27
BR112015013710A2 (pt) 2018-11-21
EP2932267B1 (en) 2020-05-06
JP2022078077A (ja) 2022-05-24
CN104871001B (zh) 2018-09-11

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