ES2803503T3 - Reconocimiento de unión a diana celular mediante un agente bioactivo usando transferencia de energía de resonancia de bioluminiscencia intracelular - Google Patents
Reconocimiento de unión a diana celular mediante un agente bioactivo usando transferencia de energía de resonancia de bioluminiscencia intracelular Download PDFInfo
- Publication number
- ES2803503T3 ES2803503T3 ES13861977T ES13861977T ES2803503T3 ES 2803503 T3 ES2803503 T3 ES 2803503T3 ES 13861977 T ES13861977 T ES 13861977T ES 13861977 T ES13861977 T ES 13861977T ES 2803503 T3 ES2803503 T3 ES 2803503T3
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- bioactive agent
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- bret
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261736429P | 2012-12-12 | 2012-12-12 | |
| US201361794461P | 2013-03-15 | 2013-03-15 | |
| US201361880048P | 2013-09-19 | 2013-09-19 | |
| PCT/US2013/074765 WO2014093677A1 (en) | 2012-12-12 | 2013-12-12 | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2803503T3 true ES2803503T3 (es) | 2021-01-27 |
Family
ID=50934963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13861977T Active ES2803503T3 (es) | 2012-12-12 | 2013-12-12 | Reconocimiento de unión a diana celular mediante un agente bioactivo usando transferencia de energía de resonancia de bioluminiscencia intracelular |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US10024862B2 (enExample) |
| EP (2) | EP2932267B1 (enExample) |
| JP (4) | JP6751294B2 (enExample) |
| KR (2) | KR102150479B1 (enExample) |
| CN (2) | CN104871001B (enExample) |
| AU (1) | AU2013359166B2 (enExample) |
| BR (1) | BR112015013710B1 (enExample) |
| CA (2) | CA2894435A1 (enExample) |
| DK (1) | DK2932267T3 (enExample) |
| ES (1) | ES2803503T3 (enExample) |
| PL (1) | PL2932267T3 (enExample) |
| SG (1) | SG11201504525VA (enExample) |
| WO (1) | WO2014093677A1 (enExample) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8524457B2 (en) | 2009-09-22 | 2013-09-03 | William Patterson | Method for the selection of specific affinity binders by homogeneous noncompetitive assay |
| CN104871001B (zh) * | 2012-12-12 | 2018-09-11 | 普洛麦格公司 | 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 |
| US10067149B2 (en) * | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| KR20230079494A (ko) | 2013-03-15 | 2023-06-07 | 프로메가 코포레이션 | 구조적 보완에 의한 바이오발광성의 활성화 |
| CN104225611B (zh) * | 2013-06-18 | 2017-06-30 | 天津键凯科技有限公司 | 达沙替尼与非线性构型聚乙二醇的结合物 |
| US11365402B2 (en) | 2014-09-12 | 2022-06-21 | Promega Corporation | Internal protein tags |
| EP3191601B1 (en) | 2014-09-12 | 2022-11-02 | Promega Corporation | Internal protein tags |
| WO2016127100A1 (en) * | 2015-02-05 | 2016-08-11 | Promega Corporation | Luciferase-based thermal shift assays |
| AU2017240233B2 (en) * | 2016-03-31 | 2022-07-14 | University Of Southern California | A highly sensitive and specific luciferase based reporter assay for antigen detection |
| CN109690316A (zh) * | 2016-05-31 | 2019-04-26 | 王帅 | 测定细胞内配体靶蛋白相互作用持久力的方法 |
| EP3535270B3 (en) * | 2016-11-01 | 2024-05-29 | Promega Corporation | Coelenterazine analogues tethered to energy acceptors |
| WO2018139614A1 (ja) * | 2017-01-27 | 2018-08-02 | 国立大学法人大阪大学 | 生体物質の検出方法、それに用いる化学発光指示薬 |
| CN110234996A (zh) * | 2017-02-03 | 2019-09-13 | 国立大学法人大阪大学 | 设备及使用其的判定系统 |
| JP7432529B2 (ja) | 2018-05-30 | 2024-02-16 | プロメガ コーポレイション | 広域スペクトルキナーゼ結合剤 |
| EP3841173A1 (en) | 2018-08-23 | 2021-06-30 | Promega Corporation | Coelenterazine analogues |
| WO2020048607A1 (en) | 2018-09-07 | 2020-03-12 | Université Libre de Bruxelles | Srsf2 as rna epigenetic factor |
| EP3887830A2 (en) | 2018-11-28 | 2021-10-06 | Promega Corporation | Reactive peptide labeling |
| JP7584433B2 (ja) | 2019-03-20 | 2024-11-15 | プロメガ コーポレイション | 光親和性プローブ |
| KR20220007058A (ko) * | 2019-04-10 | 2022-01-18 | 프로메가 코포레이션 | 생물발광을 이용하는 분석물 검출을 위한 조성물 및 방법 |
| CN112147114B (zh) * | 2019-06-27 | 2024-06-25 | 成都先导药物开发股份有限公司 | 一种利用荧光标记化合物确定化合物在活细胞中与靶标相互作用的方法 |
| WO2021119149A1 (en) | 2019-12-10 | 2021-06-17 | Promega Corporation | Compositions and methods for bioluminescent detection using multifunctional probes |
| JP2023540934A (ja) * | 2020-08-28 | 2023-09-27 | プロメガ コーポレイション | Ｒａｓタンパク質のターゲットエンゲージメントアッセイ |
| CN115161296B (zh) * | 2021-04-01 | 2024-08-13 | 深圳华大生命科学研究院 | 一种刺虾荧光素酶Nluc的突变体及其应用 |
| CN114394970B (zh) * | 2022-01-05 | 2023-06-06 | 山东大学 | 一种酯类Furimazine衍生物及制备方法与应用 |
| CN114813706B (zh) * | 2022-06-29 | 2022-12-13 | 国科大杭州高等研究院 | 一种血细胞高光谱光镊捕获能量共振转移分析仪 |
| CN120051474A (zh) | 2022-10-14 | 2025-05-27 | 普罗美加公司 | 腔肠素类似物 |
| CN116354927B (zh) * | 2023-02-21 | 2025-02-07 | 杭州医学院 | 以烷烃或脂肪酸为连接链的hdac降解剂及其制备方法和应用 |
| WO2024229422A2 (en) * | 2023-05-04 | 2024-11-07 | Promega Corporation | Complementation-based tags and reporters for dual-modality labeling |
| KR20250004513A (ko) | 2023-06-29 | 2025-01-08 | 부산대학교 산학협력단 | Bret-기반 rab11a 활성 바이오센서 및 이를 포함하는 세포주 |
| CN117330755B (zh) * | 2023-08-25 | 2024-03-26 | 广东省农业科学院农业生物基因研究中心 | 一种基于酶催化生物荧光定量检测dna-蛋白质相互作用的方法 |
| KR102802452B1 (ko) | 2023-11-07 | 2025-05-08 | 대한민국 | LgBiT 단백질을 발현하는 세포주 및 HiBiT 단백질을 발현하는 구제역 바이러스를 이용한 구제역 바이러스 감염 여부 확인방법 |
| US20250206747A1 (en) | 2023-12-20 | 2025-06-26 | Promega Corporation | Coelenterazine analogues |
| GB202410534D0 (en) | 2024-07-18 | 2024-09-04 | Nrg Therapeutics Ltd | Novel method and uses |
| CN119709661B (zh) * | 2024-12-30 | 2025-10-10 | 佰翱得(无锡)新药开发有限公司 | 一种荧光素酶OLuc突变体及其应用 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5928888A (en) * | 1996-09-26 | 1999-07-27 | Aurora Biosciences Corporation | Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities |
| CA2335305C (en) | 1998-06-16 | 2006-05-23 | Biosignal Packard Inc. | A bioluminescence resonance energy transfer (bret) system and its use |
| US20030203404A1 (en) | 1999-12-22 | 2003-10-30 | Erik Joly | Bioluminescence resonance energy transfer( bret) system with broad spectral resolution between donor and acceptor emission wavelengths and its use |
| EP2085781B2 (en) | 2000-10-06 | 2020-03-11 | Life Technologies Corporation | Transfectable micelles containing semiconductor nanocrystals |
| DE10058091A1 (de) * | 2000-11-23 | 2002-06-06 | Bayer Ag | Isolierte Luziferasen Lu164, LuAL und Lu22, sowie deren Verwendung |
| US20060099646A1 (en) | 2002-10-11 | 2006-05-11 | Anders Heding | Bret assay |
| GB0307559D0 (en) | 2003-04-02 | 2003-05-07 | Univ Nottingham | Fluorescently tagged ligands |
| CN100473988C (zh) * | 2004-07-08 | 2009-04-01 | 厦门大学 | 具有荧光共振能量转移特点的蛋白组合及其用途 |
| US9029097B2 (en) | 2005-02-16 | 2015-05-12 | Universite De Montreal | Biosensors for monitoring receptor-mediated G-protein activation |
| GB0625678D0 (en) * | 2006-12-21 | 2007-01-31 | Lux Biotechnology Ltd | Composition and method for detection of demineralisation |
| US20080299592A1 (en) * | 2007-03-02 | 2008-12-04 | Miller Stephen C | Red-Shifted Luciferase |
| EP2006686A1 (en) * | 2007-06-22 | 2008-12-24 | Institut Pasteur | Method for screening for selective modulator of the NF-kB pathway activation |
| WO2009061413A2 (en) * | 2007-11-05 | 2009-05-14 | Promega Corporation | Hybrid fusion reporter and uses thereof |
| US8962341B2 (en) * | 2008-12-12 | 2015-02-24 | The Commonwealth Medical College | Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis |
| PL3744834T3 (pl) | 2009-05-01 | 2026-01-05 | Promega Corporation | Syntetyczne lucyferazy z krewetki oplορηorus emitującej bardziej intensywne światło |
| US8263350B2 (en) * | 2009-06-29 | 2012-09-11 | The University Of Chicago | Molecular affinity clamp technology and uses thereof |
| EP2635582B1 (en) | 2010-11-02 | 2016-01-27 | Promega Corporation | Novel coelenterazine substrates and methods of use |
| WO2013078244A1 (en) * | 2011-11-21 | 2013-05-30 | Promega Corporation | Carboxy x rhodamine analogs |
| CN102798717B (zh) * | 2012-06-15 | 2014-11-26 | 杭州师范大学 | 一种o6-甲基鸟嘌呤-dna甲基转移酶活性检测方法 |
| US10067149B2 (en) | 2012-12-12 | 2018-09-04 | Promega Corporation | Recognition of cellular target binding by a bioactive agent using intracellular bioluminescence resonance energy transfer |
| CN104871001B (zh) | 2012-12-12 | 2018-09-11 | 普洛麦格公司 | 使用细胞内生物发光共振能量转移识别生物活性剂的细胞靶标结合 |
-
2013
- 2013-12-12 CN CN201380064252.4A patent/CN104871001B/zh active Active
- 2013-12-12 EP EP13861977.0A patent/EP2932267B1/en active Active
- 2013-12-12 WO PCT/US2013/074765 patent/WO2014093677A1/en not_active Ceased
- 2013-12-12 CA CA2894435A patent/CA2894435A1/en active Pending
- 2013-12-12 JP JP2015547562A patent/JP6751294B2/ja active Active
- 2013-12-12 ES ES13861977T patent/ES2803503T3/es active Active
- 2013-12-12 KR KR1020157018230A patent/KR102150479B1/ko active Active
- 2013-12-12 PL PL13861977T patent/PL2932267T3/pl unknown
- 2013-12-12 EP EP20169678.8A patent/EP3712154A1/en active Pending
- 2013-12-12 SG SG11201504525VA patent/SG11201504525VA/en unknown
- 2013-12-12 KR KR1020207024622A patent/KR102292431B1/ko active Active
- 2013-12-12 US US14/104,860 patent/US10024862B2/en active Active
- 2013-12-12 AU AU2013359166A patent/AU2013359166B2/en active Active
- 2013-12-12 DK DK13861977.0T patent/DK2932267T3/da active
- 2013-12-12 BR BR112015013710-5A patent/BR112015013710B1/pt active IP Right Grant
- 2013-12-12 CN CN201810902469.2A patent/CN109612981B/zh active Active
- 2013-12-12 CA CA3174484A patent/CA3174484A1/en active Pending
-
2019
- 2019-01-21 JP JP2019007974A patent/JP7092691B2/ja active Active
-
2022
- 2022-02-14 JP JP2022020320A patent/JP7393451B2/ja active Active
-
2023
- 2023-08-22 JP JP2023134744A patent/JP7676487B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2932267B1 (en) | 2020-05-06 |
| JP2023179413A (ja) | 2023-12-19 |
| CN104871001A (zh) | 2015-08-26 |
| EP2932267A4 (en) | 2016-06-22 |
| AU2013359166A1 (en) | 2015-07-09 |
| CN109612981A (zh) | 2019-04-12 |
| WO2014093677A1 (en) | 2014-06-19 |
| SG11201504525VA (en) | 2015-07-30 |
| KR20150094696A (ko) | 2015-08-19 |
| KR102150479B1 (ko) | 2020-09-01 |
| CA3174484A1 (en) | 2014-06-19 |
| PL2932267T3 (pl) | 2020-10-19 |
| JP2022078077A (ja) | 2022-05-24 |
| AU2013359166B2 (en) | 2019-09-19 |
| JP2019071899A (ja) | 2019-05-16 |
| JP6751294B2 (ja) | 2020-09-02 |
| BR112015013710B1 (pt) | 2022-08-09 |
| CN109612981B (zh) | 2022-05-13 |
| EP3712154A1 (en) | 2020-09-23 |
| CA2894435A1 (en) | 2014-06-19 |
| KR102292431B1 (ko) | 2021-08-20 |
| JP2016500263A (ja) | 2016-01-12 |
| US20140194307A1 (en) | 2014-07-10 |
| JP7676487B2 (ja) | 2025-05-14 |
| US10024862B2 (en) | 2018-07-17 |
| DK2932267T3 (da) | 2020-07-20 |
| EP2932267A1 (en) | 2015-10-21 |
| JP7393451B2 (ja) | 2023-12-06 |
| JP7092691B2 (ja) | 2022-06-28 |
| BR112015013710A2 (pt) | 2018-11-21 |
| CN104871001B (zh) | 2018-09-11 |
| KR20200104928A (ko) | 2020-09-04 |
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