JP2015071629A5 - - Google Patents

Download PDF

Info

Publication number
JP2015071629A5
JP2015071629A5 JP2014246058A JP2014246058A JP2015071629A5 JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5 JP 2014246058 A JP2014246058 A JP 2014246058A JP 2014246058 A JP2014246058 A JP 2014246058A JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5
Authority
JP
Japan
Prior art keywords
eluate
sample
flow
protein
hcp
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2014246058A
Other languages
English (en)
Japanese (ja)
Other versions
JP6254072B2 (ja
JP2015071629A (ja
Filing date
Publication date
Application filed filed Critical
Publication of JP2015071629A publication Critical patent/JP2015071629A/ja
Publication of JP2015071629A5 publication Critical patent/JP2015071629A5/ja
Application granted granted Critical
Publication of JP6254072B2 publication Critical patent/JP6254072B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2014246058A 2011-08-19 2014-12-04 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 Active JP6254072B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201161575349P 2011-08-19 2011-08-19
US61/575,349 2011-08-19
US201261666240P 2012-06-29 2012-06-29
US61/666,240 2012-06-29

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2012179197A Division JP5938295B2 (ja) 2011-08-19 2012-08-13 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法

Publications (3)

Publication Number Publication Date
JP2015071629A JP2015071629A (ja) 2015-04-16
JP2015071629A5 true JP2015071629A5 (https=) 2016-03-31
JP6254072B2 JP6254072B2 (ja) 2017-12-27

Family

ID=47076067

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2012179197A Active JP5938295B2 (ja) 2011-08-19 2012-08-13 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法
JP2014246058A Active JP6254072B2 (ja) 2011-08-19 2014-12-04 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法
JP2016022509A Active JP6339120B2 (ja) 2011-08-19 2016-02-09 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法
JP2018017028A Active JP6743074B2 (ja) 2011-08-19 2018-02-02 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2012179197A Active JP5938295B2 (ja) 2011-08-19 2012-08-13 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法

Family Applications After (2)

Application Number Title Priority Date Filing Date
JP2016022509A Active JP6339120B2 (ja) 2011-08-19 2016-02-09 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法
JP2018017028A Active JP6743074B2 (ja) 2011-08-19 2018-02-02 タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法

Country Status (8)

Country Link
US (3) US9096648B2 (https=)
EP (2) EP2574618B1 (https=)
JP (4) JP5938295B2 (https=)
KR (1) KR101484659B1 (https=)
CN (3) CN111423488B (https=)
ES (2) ES2539232T3 (https=)
TW (1) TWI574975B (https=)
WO (1) WO2013028330A2 (https=)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10507409B2 (en) * 2016-03-12 2019-12-17 Spf Technologies, Llc Hyper-productive chromatography system and process
US11395980B2 (en) 2010-01-25 2022-07-26 Spf Technologies Llc Chromatographic cassette
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
WO2013075740A1 (en) 2011-11-23 2013-05-30 Sanofi Antibody purification method
CN104411820B (zh) * 2012-06-29 2017-05-03 Emd密理博公司 在蛋白纯化过程中灭活病毒的方法
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US9650411B2 (en) * 2012-08-07 2017-05-16 Kyowa Hakko Kirin Co., Ltd. Method of purifying protein
EP2961760B1 (en) * 2013-02-26 2021-03-24 EMD Millipore Corporation Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions
ES2843786T3 (es) 2013-03-13 2021-07-20 Seagen Inc Filtración con carbono activado para la purificación de ADC de benzodiazepina
NO2994485T3 (https=) 2013-05-06 2018-06-09
JP2016529885A (ja) * 2013-07-12 2016-09-29 イー・エム・デイー・ミリポア・コーポレイシヨン 活性炭を使用して標的タンパク質を含む試料からのウイルス除去を決定する方法
WO2015005960A1 (en) * 2013-07-12 2015-01-15 Emd Millipore Corporation Removal of fragments from a sample containing a target protein using activated carbon
EP4331605A3 (en) * 2013-09-13 2024-05-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
CN103497248B (zh) * 2013-09-22 2016-08-10 中国抗体制药有限公司 一种从细胞培养上清中分离纯化抗体的方法
EP2986307A4 (en) 2014-01-03 2017-04-12 Bio-rad Laboratories, Inc. Removal of impurities from protein a eluates
CN106103477B (zh) * 2014-03-04 2021-08-17 默克专利股份公司 稳健的抗体纯化
WO2015137860A1 (en) * 2014-03-14 2015-09-17 Ge Healthcare Bio-Sciences Ab Separation matrices for purification of biological particles
KR102398719B1 (ko) * 2014-11-06 2022-05-16 메르크 파텐트 게엠베하 침출물 및/또는 추출물 제거용 활성탄
WO2016146594A1 (en) * 2015-03-13 2016-09-22 Novimmune Sa Methods of purifying bispecific antibodies
LT4209499T (lt) 2015-08-13 2024-11-25 Amgen Inc. Antigeną surišančių baltymų įkrautas giluminis filtravimas
EP3337819B1 (en) * 2015-08-20 2024-02-21 F. Hoffmann-La Roche AG Method of purifying for producing recombinant polypeptides using fkpa
US12409440B1 (en) 2016-01-26 2025-09-09 Spf Technologies Llc Separation module
EP3430072B1 (en) * 2016-03-17 2020-07-01 Merck Patent GmbH Method for purifying poloxamers
WO2017168296A1 (en) * 2016-03-29 2017-10-05 Navya Biologicals Pvt. Ltd A process for purification of fc-fusion proteins
CA3016847C (en) 2016-04-01 2023-12-19 Ucb Biopharma Sprl Method for protein purification
WO2018092691A1 (ja) * 2016-11-18 2018-05-24 Jnc株式会社 抗体の精製方法
SG11201908879RA (en) * 2017-04-01 2019-10-30 Massachusetts Inst Technology Systems and methods for manufacturing biologically-produced products
KR20200010321A (ko) * 2017-05-17 2020-01-30 엠에스디 웰컴 트러스트 힐레맨 랩스 피브이티. 리미티드 세균성 다당류의 정제
CN107236038B (zh) * 2017-06-20 2020-11-27 华兰生物工程股份有限公司 微晶纤维素提高蛋白质澄清度的方法及其产品和应用
WO2019040671A1 (en) * 2017-08-22 2019-02-28 Biogen Ma Inc. METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT
KR20200042532A (ko) 2017-08-31 2020-04-23 메사추세츠 인스티튜트 오브 테크놀로지 생물학적으로 생성된 생성물을 제조하기 위한 중공 섬유 멤브레인 여과 시스템 및 방법
US11071927B2 (en) * 2017-11-15 2021-07-27 Arup Laboratories, Inc. Separating and quantifying unbound target analytes from biological samples
CN109929038B (zh) * 2017-12-15 2020-10-09 山东博安生物技术有限公司 Vegf捕获剂融合蛋白的纯化方法
US12343702B2 (en) 2018-03-08 2025-07-01 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
EP3546475A1 (en) * 2018-03-27 2019-10-02 Sanofi Full flow-through process for purifying recombinant proteins
CN112566930A (zh) * 2018-06-19 2021-03-26 百时美施贵宝公司 使用色谱纯化蛋白质的方法
US11391707B2 (en) * 2018-07-27 2022-07-19 Waters Technologies Corporation Liquid chromatography/mass spectrometry methods for the analysis of polar molecules
WO2020036869A1 (en) 2018-08-16 2020-02-20 Emd Millipore Corporation Closed bioprocessing device
CN111215027A (zh) * 2018-11-26 2020-06-02 中国科学院大连化学物理研究所 一种水热碳微球色谱介质的修饰方法
WO2020117760A1 (en) * 2018-12-03 2020-06-11 Duke University Purification method for recombinant proteins and nanoparticles
KR102120675B1 (ko) * 2018-12-05 2020-06-09 유바이오주식회사 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물
CN118904316A (zh) 2018-12-12 2024-11-08 唐纳森公司 亲和膜及其制备方法
CA3127174A1 (en) * 2019-01-23 2020-07-30 Daiichi Sankyo Company, Limited Methods for purifying antibodies comprising of a process by using activated carbon materials
EP3947694A4 (en) * 2019-04-02 2023-01-04 Council of Scientific & Industrial Research CLONING AND EXPRESSION OF AN IN VIVO REFOLDED ANTIBODY FRAGMENT
CN114981286B (zh) 2019-05-03 2026-02-13 豪夫迈·罗氏有限公司 降低从纯化平台获得的组合物中酶水解活性速率的方法
EP4025336A4 (en) 2019-09-05 2024-01-17 Bio-Rad Laboratories, Inc. ANION EXCHANGE/HYDROPHOBIC RESINS FOR MIXED MODE CHROMATOGRAPHY
US11180540B2 (en) 2019-12-06 2021-11-23 Regeneron Pharmaceuticals, Inc. Anti-VEGF protein compositions and methods for producing the same
US20210178334A1 (en) * 2019-12-16 2021-06-17 Dionex Corporation Electrolytic eluent generators with stabilized operating voltages
AU2021208515A1 (en) 2020-01-15 2022-08-04 F. Hoffmann-La Roche Ag Methods to decrease impurities from recombinant protein manufacturing processes
US20230227496A1 (en) * 2020-04-13 2023-07-20 3M Innovative Properties Company Flow-through processes and devices for purifying a target molecule
JP2023525034A (ja) 2020-05-08 2023-06-14 リジェネロン・ファーマシューティカルズ・インコーポレイテッド Vegfトラップおよびミニトラップならびに眼障害およびがんの治療方法
WO2022094116A1 (en) * 2020-10-30 2022-05-05 Genentech, Inc. Purification platforms for obtaining pharmaceutical compositions having a reduced hydrolytic enzyme activity rate
JP2023549938A (ja) * 2020-11-20 2023-11-29 アレス トレーディング ソシエテ アノニム タンパク質を精製するための緩衝液と方法
KR20230125282A (ko) 2020-12-28 2023-08-29 플라즈마 테크놀로지스, 엘엘씨 면역글로불린 G의 공정 규모 단리를 위한 시스템 및방법 (Systems and Methods for Process Scale Isolation of Immunoglobulin G)
CN112870979B (zh) * 2020-12-31 2022-12-23 内蒙古金达威药业有限公司 一种β-烟酰胺单核苷酸的分离纯化方法
CN115326937B (zh) * 2021-05-11 2024-06-18 山东省食品药品检验研究院 一种基因毒杂质捕捉用固相探针及其使用方法与应用
AU2021471146A1 (en) 2021-10-27 2024-06-13 Plasma Technologies, Llc Compositions and methods for isolating proteins
CN116120392B (zh) * 2023-04-18 2023-08-01 上海健士拜生物科技有限公司 聚体蛋白的纯化方法
CN118126110B (zh) * 2024-05-10 2024-08-06 江苏赛孚士生物技术有限公司 一种纯化单克隆抗体的方法

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US31093A (en) 1861-01-08 Mode of constructing iron pavements
USRE31093E (en) 1978-05-10 1982-11-30 Westvaco Corporation Process for making activated carbon with control of metal ion concentration in phosphoric acid
JPS5918731A (ja) 1982-07-21 1984-01-31 Toray Ind Inc イオン交換繊維
US4639513A (en) 1984-02-02 1987-01-27 Cuno Inc. Intravenously injectable immunoglobulin G (IGG) and method for producing same
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE3927112C1 (https=) * 1989-08-17 1990-10-25 Biotest Pharma Gmbh, 6072 Dreieich, De
GB8923662D0 (en) 1989-10-20 1989-12-06 Norit Uk Ltd A method of producing granular activated carbon
JPH0778025B2 (ja) * 1990-03-20 1995-08-23 日本赤十字社 免疫グロブリンgの製造方法
US5204310A (en) 1992-02-21 1993-04-20 Westvaco Corporation High activity, high density activated carbon
US5612558A (en) 1995-11-15 1997-03-18 Micron Technology, Inc. Hemispherical grained silicon on refractory metal nitride
US20020056686A1 (en) * 1996-06-14 2002-05-16 Agathagelos Kyrlidis Chromatography and other adsorptions using modified carbon adsorbents
DE29801192U1 (de) 1998-01-26 1998-08-20 Hans Bernstein Spezialfabrik für Schaltkontakte GmbH & Co, 32457 Porta Westfalica Radiusbetätiger für Sicherheitsschalter
DE19935160A1 (de) 1999-07-27 2001-02-15 Grove Us Llc Shady Grove Vorrichtung zur Erhöhung der Lenkfähigkeit getriebener Achsen
US6569910B1 (en) 1999-10-27 2003-05-27 Basf Aktiengesellschaft Ion exchange resins and methods of making the same
US20040028901A1 (en) 2002-02-25 2004-02-12 Rumpf Frederick H. Compositions comprising continuous networks and monoliths
KR101035043B1 (ko) 2002-07-01 2011-05-19 아르키온 라이프 사이언씨즈 엘엘씨 글루코사민 및 n-아세틸글루코사민의 제조를 위한 물질및 공정
GB0304576D0 (en) 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
AU2004215653B2 (en) 2003-02-28 2011-03-17 Lonza Biologics Plc. Antibody purification by protein A and ion exchange chromatography
WO2005077130A2 (en) 2004-02-11 2005-08-25 Tanox, Inc. A method for the removal of aggregate proteins from recombinant samples using ion exchange chromatography
SE0402558D0 (sv) * 2004-10-21 2004-10-21 Amersham Biosciences Ab A process for the purification of antibodies
US7390403B2 (en) 2004-03-19 2008-06-24 Millipore Corporation Prefilter system for biological systems
JP2008526201A (ja) * 2004-12-30 2008-07-24 シェーリング−プラウ・リミテッド 中和エピトープベースの成長増強ワクチン
WO2007063129A2 (en) 2005-12-02 2007-06-07 Novozymes A/S Insolation of peptides , polypeptides and proteins
EP1981704A4 (en) 2005-12-08 2011-06-08 Waters Technologies Corp DEVICE AND METHODS FOR PREPARING SAMPLES OF PEPTIDES AND PROTEINS FROM A SOLUTION
EA017733B1 (ru) * 2006-08-28 2013-02-28 Арес Трейдинг С.А. СПОСОБ ОЧИСТКИ Fc-СЛИТЫХ БЕЛКОВ
CA2570956C (en) * 2006-12-12 2014-04-01 Norgen Biotek Corporation Method of using silicon carbide for removal of adventitious agents
BRPI0812288A2 (pt) * 2007-06-01 2014-11-25 Hoffmann La Roche Purificação de imunoglobulina.
JP2010279461A (ja) 2009-06-03 2010-12-16 Daicen Membrane Systems Ltd 人工透析液又は人工透析用水の清浄化方法
US8945895B2 (en) 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
MX346115B (es) 2009-08-06 2017-03-08 Genentech Inc * Metodo para mejorar la eliminación de virus en la purificacion proteica.
US8536316B2 (en) 2009-08-07 2013-09-17 Emd Millipore Corporation Methods for purifying a target protein from one or more impurities in a sample
US9527010B2 (en) 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method
WO2011090720A2 (en) 2009-12-29 2011-07-28 Dr. Reddy's Laboratories Ltd Purification of proteins
WO2013028334A2 (en) * 2011-08-19 2013-02-28 Emd Millipore Corporation Use of small molecules in methods for purification of biomolecules
US9096648B2 (en) * 2011-08-19 2015-08-04 Emd Millipore Corporation Methods of reducing level of one or more impurities in a sample during protein purification
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
WO2015005960A1 (en) * 2013-07-12 2015-01-15 Emd Millipore Corporation Removal of fragments from a sample containing a target protein using activated carbon

Similar Documents

Publication Publication Date Title
JP2015071629A5 (https=)
JP2012018171A5 (https=)
JP5148484B2 (ja) クロマトグラフィーマトリックスの再生
Zhou et al. Basic concepts in Q membrane chromatography for large‐scale antibody production
Jandera Advances in the development of organic polymer monolithic columns and their applications in food analysis—a review
CN105777896B (zh) 一种抗体酸性峰的纯化方法
KR20170121731A (ko) 관류 공정의 용량을 증가시키는 방법
SG150458A1 (en) Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
RS56022B1 (sr) Podloga za hromatografiju
Hajizadeh et al. Evaluation of selective composite cryogel for bromate removal from drinking water
Zahoor Removal of Pesticides from Water Using Granular Activated Carbon and Ultrafiltration Membrane--A Pilot Plant Study
Dong et al. Isolation of immunoglobulin G from bovine milk whey by poly (hydroxyethyl methacrylate)‐based anion‐exchange cryogel
Baydemir et al. Composite cryogels for lysozyme purification
US7488422B2 (en) Method and apparatus for separating a target molecule from a liquid mixture
JP2017003527A (ja) 親水性相互作用クロマトグラフィー用充填剤、及び親水性化合物の分離方法
Yoon et al. High-capacity/high-rate hybrid column for high-performance ion exchange
Ünlü et al. Investigation of protein adsorption performance of Ni2+‐attached diatomite particles embedded in composite monolithic cryogels
Feast et al. Purification of his-tagged proteins using printed monolith adsorption columns
Rong et al. Transport and release behaviors of PFOA in saturated and unsaturated porous media with biochar amendment
Alkan et al. Cu2+-attached pumice particles embedded composite cryogels for protein purification
JP7057912B2 (ja) タンパク質の分離精製方法および装置
DK2748181T3 (en) PROCEDURE FOR CLEANING THE CLEANED PRO-INSULIN
CN103212387B (zh) 一种重金属离子吸附剂及其制备方法和应用
CN107118116A (zh) 一种利用大孔吸附树脂分离纯化5‑氨基戊酸的方法
Yoshimoto et al. Connected flow-through chromatography processes as continuous downstream processing of proteins