JP2015071629A5 - - Google Patents
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- Publication number
- JP2015071629A5 JP2015071629A5 JP2014246058A JP2014246058A JP2015071629A5 JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5 JP 2014246058 A JP2014246058 A JP 2014246058A JP 2014246058 A JP2014246058 A JP 2014246058A JP 2015071629 A5 JP2015071629 A5 JP 2015071629A5
- Authority
- JP
- Japan
- Prior art keywords
- eluate
- sample
- flow
- protein
- hcp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 14
- 102000004169 proteins and genes Human genes 0.000 claims 11
- 108090000623 proteins and genes Proteins 0.000 claims 11
- 239000002609 medium Substances 0.000 claims 7
- 238000005349 anion exchange Methods 0.000 claims 4
- 239000003575 carbonaceous material Substances 0.000 claims 4
- 239000002775 capsule Substances 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 239000012501 chromatography medium Substances 0.000 claims 2
- 239000012528 membrane Substances 0.000 claims 2
- 239000011148 porous material Substances 0.000 claims 2
- 238000000746 purification Methods 0.000 claims 2
- 238000011100 viral filtration Methods 0.000 claims 2
- 239000006096 absorbing agent Substances 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- 239000012535 impurity Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 230000003068 static effect Effects 0.000 claims 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161575349P | 2011-08-19 | 2011-08-19 | |
| US61/575,349 | 2011-08-19 | ||
| US201261666240P | 2012-06-29 | 2012-06-29 | |
| US61/666,240 | 2012-06-29 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Division JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015071629A JP2015071629A (ja) | 2015-04-16 |
| JP2015071629A5 true JP2015071629A5 (https=) | 2016-03-31 |
| JP6254072B2 JP6254072B2 (ja) | 2017-12-27 |
Family
ID=47076067
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2014246058A Active JP6254072B2 (ja) | 2011-08-19 | 2014-12-04 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012179197A Active JP5938295B2 (ja) | 2011-08-19 | 2012-08-13 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016022509A Active JP6339120B2 (ja) | 2011-08-19 | 2016-02-09 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
| JP2018017028A Active JP6743074B2 (ja) | 2011-08-19 | 2018-02-02 | タンパク質精製中に試料中の1または複数の不純物のレベルを低下させる方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US9096648B2 (https=) |
| EP (2) | EP2574618B1 (https=) |
| JP (4) | JP5938295B2 (https=) |
| KR (1) | KR101484659B1 (https=) |
| CN (3) | CN111423488B (https=) |
| ES (2) | ES2539232T3 (https=) |
| TW (1) | TWI574975B (https=) |
| WO (1) | WO2013028330A2 (https=) |
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| US10507409B2 (en) * | 2016-03-12 | 2019-12-17 | Spf Technologies, Llc | Hyper-productive chromatography system and process |
| US11395980B2 (en) | 2010-01-25 | 2022-07-26 | Spf Technologies Llc | Chromatographic cassette |
| US9096648B2 (en) * | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| CN104411820B (zh) * | 2012-06-29 | 2017-05-03 | Emd密理博公司 | 在蛋白纯化过程中灭活病毒的方法 |
| EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US9650411B2 (en) * | 2012-08-07 | 2017-05-16 | Kyowa Hakko Kirin Co., Ltd. | Method of purifying protein |
| EP2961760B1 (en) * | 2013-02-26 | 2021-03-24 | EMD Millipore Corporation | Selective removal of a protein from a mixture of proteins using activated carbon by adjusting solution conditions |
| ES2843786T3 (es) | 2013-03-13 | 2021-07-20 | Seagen Inc | Filtración con carbono activado para la purificación de ADC de benzodiazepina |
| NO2994485T3 (https=) | 2013-05-06 | 2018-06-09 | ||
| JP2016529885A (ja) * | 2013-07-12 | 2016-09-29 | イー・エム・デイー・ミリポア・コーポレイシヨン | 活性炭を使用して標的タンパク質を含む試料からのウイルス除去を決定する方法 |
| WO2015005960A1 (en) * | 2013-07-12 | 2015-01-15 | Emd Millipore Corporation | Removal of fragments from a sample containing a target protein using activated carbon |
| EP4331605A3 (en) * | 2013-09-13 | 2024-05-22 | F. Hoffmann-La Roche AG | Methods and compositions comprising purified recombinant polypeptides |
| CN103497248B (zh) * | 2013-09-22 | 2016-08-10 | 中国抗体制药有限公司 | 一种从细胞培养上清中分离纯化抗体的方法 |
| EP2986307A4 (en) | 2014-01-03 | 2017-04-12 | Bio-rad Laboratories, Inc. | Removal of impurities from protein a eluates |
| CN106103477B (zh) * | 2014-03-04 | 2021-08-17 | 默克专利股份公司 | 稳健的抗体纯化 |
| WO2015137860A1 (en) * | 2014-03-14 | 2015-09-17 | Ge Healthcare Bio-Sciences Ab | Separation matrices for purification of biological particles |
| KR102398719B1 (ko) * | 2014-11-06 | 2022-05-16 | 메르크 파텐트 게엠베하 | 침출물 및/또는 추출물 제거용 활성탄 |
| WO2016146594A1 (en) * | 2015-03-13 | 2016-09-22 | Novimmune Sa | Methods of purifying bispecific antibodies |
| LT4209499T (lt) | 2015-08-13 | 2024-11-25 | Amgen Inc. | Antigeną surišančių baltymų įkrautas giluminis filtravimas |
| EP3337819B1 (en) * | 2015-08-20 | 2024-02-21 | F. Hoffmann-La Roche AG | Method of purifying for producing recombinant polypeptides using fkpa |
| US12409440B1 (en) | 2016-01-26 | 2025-09-09 | Spf Technologies Llc | Separation module |
| EP3430072B1 (en) * | 2016-03-17 | 2020-07-01 | Merck Patent GmbH | Method for purifying poloxamers |
| WO2017168296A1 (en) * | 2016-03-29 | 2017-10-05 | Navya Biologicals Pvt. Ltd | A process for purification of fc-fusion proteins |
| CA3016847C (en) | 2016-04-01 | 2023-12-19 | Ucb Biopharma Sprl | Method for protein purification |
| WO2018092691A1 (ja) * | 2016-11-18 | 2018-05-24 | Jnc株式会社 | 抗体の精製方法 |
| SG11201908879RA (en) * | 2017-04-01 | 2019-10-30 | Massachusetts Inst Technology | Systems and methods for manufacturing biologically-produced products |
| KR20200010321A (ko) * | 2017-05-17 | 2020-01-30 | 엠에스디 웰컴 트러스트 힐레맨 랩스 피브이티. 리미티드 | 세균성 다당류의 정제 |
| CN107236038B (zh) * | 2017-06-20 | 2020-11-27 | 华兰生物工程股份有限公司 | 微晶纤维素提高蛋白质澄清度的方法及其产品和应用 |
| WO2019040671A1 (en) * | 2017-08-22 | 2019-02-28 | Biogen Ma Inc. | METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT |
| KR20200042532A (ko) | 2017-08-31 | 2020-04-23 | 메사추세츠 인스티튜트 오브 테크놀로지 | 생물학적으로 생성된 생성물을 제조하기 위한 중공 섬유 멤브레인 여과 시스템 및 방법 |
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| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| US12343702B2 (en) | 2018-03-08 | 2025-07-01 | Bio-Rad Laboratories, Inc. | Anionic exchange-hydrophobic mixed mode chromatography resin |
| EP3546475A1 (en) * | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| CN112566930A (zh) * | 2018-06-19 | 2021-03-26 | 百时美施贵宝公司 | 使用色谱纯化蛋白质的方法 |
| US11391707B2 (en) * | 2018-07-27 | 2022-07-19 | Waters Technologies Corporation | Liquid chromatography/mass spectrometry methods for the analysis of polar molecules |
| WO2020036869A1 (en) | 2018-08-16 | 2020-02-20 | Emd Millipore Corporation | Closed bioprocessing device |
| CN111215027A (zh) * | 2018-11-26 | 2020-06-02 | 中国科学院大连化学物理研究所 | 一种水热碳微球色谱介质的修饰方法 |
| WO2020117760A1 (en) * | 2018-12-03 | 2020-06-11 | Duke University | Purification method for recombinant proteins and nanoparticles |
| KR102120675B1 (ko) * | 2018-12-05 | 2020-06-09 | 유바이오주식회사 | 바이러스 클리어런스 공정을 포함하는 봉독의 정제방법 및 이를 이용한 염증성 질환 예방 또는 치료용 조성물 |
| CN118904316A (zh) | 2018-12-12 | 2024-11-08 | 唐纳森公司 | 亲和膜及其制备方法 |
| CA3127174A1 (en) * | 2019-01-23 | 2020-07-30 | Daiichi Sankyo Company, Limited | Methods for purifying antibodies comprising of a process by using activated carbon materials |
| EP3947694A4 (en) * | 2019-04-02 | 2023-01-04 | Council of Scientific & Industrial Research | CLONING AND EXPRESSION OF AN IN VIVO REFOLDED ANTIBODY FRAGMENT |
| CN114981286B (zh) | 2019-05-03 | 2026-02-13 | 豪夫迈·罗氏有限公司 | 降低从纯化平台获得的组合物中酶水解活性速率的方法 |
| EP4025336A4 (en) | 2019-09-05 | 2024-01-17 | Bio-Rad Laboratories, Inc. | ANION EXCHANGE/HYDROPHOBIC RESINS FOR MIXED MODE CHROMATOGRAPHY |
| US11180540B2 (en) | 2019-12-06 | 2021-11-23 | Regeneron Pharmaceuticals, Inc. | Anti-VEGF protein compositions and methods for producing the same |
| US20210178334A1 (en) * | 2019-12-16 | 2021-06-17 | Dionex Corporation | Electrolytic eluent generators with stabilized operating voltages |
| AU2021208515A1 (en) | 2020-01-15 | 2022-08-04 | F. Hoffmann-La Roche Ag | Methods to decrease impurities from recombinant protein manufacturing processes |
| US20230227496A1 (en) * | 2020-04-13 | 2023-07-20 | 3M Innovative Properties Company | Flow-through processes and devices for purifying a target molecule |
| JP2023525034A (ja) | 2020-05-08 | 2023-06-14 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | Vegfトラップおよびミニトラップならびに眼障害およびがんの治療方法 |
| WO2022094116A1 (en) * | 2020-10-30 | 2022-05-05 | Genentech, Inc. | Purification platforms for obtaining pharmaceutical compositions having a reduced hydrolytic enzyme activity rate |
| JP2023549938A (ja) * | 2020-11-20 | 2023-11-29 | アレス トレーディング ソシエテ アノニム | タンパク質を精製するための緩衝液と方法 |
| KR20230125282A (ko) | 2020-12-28 | 2023-08-29 | 플라즈마 테크놀로지스, 엘엘씨 | 면역글로불린 G의 공정 규모 단리를 위한 시스템 및방법 (Systems and Methods for Process Scale Isolation of Immunoglobulin G) |
| CN112870979B (zh) * | 2020-12-31 | 2022-12-23 | 内蒙古金达威药业有限公司 | 一种β-烟酰胺单核苷酸的分离纯化方法 |
| CN115326937B (zh) * | 2021-05-11 | 2024-06-18 | 山东省食品药品检验研究院 | 一种基因毒杂质捕捉用固相探针及其使用方法与应用 |
| AU2021471146A1 (en) | 2021-10-27 | 2024-06-13 | Plasma Technologies, Llc | Compositions and methods for isolating proteins |
| CN116120392B (zh) * | 2023-04-18 | 2023-08-01 | 上海健士拜生物科技有限公司 | 聚体蛋白的纯化方法 |
| CN118126110B (zh) * | 2024-05-10 | 2024-08-06 | 江苏赛孚士生物技术有限公司 | 一种纯化单克隆抗体的方法 |
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| US31093A (en) | 1861-01-08 | Mode of constructing iron pavements | ||
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| US9096648B2 (en) * | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
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| EP2682168A1 (en) * | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| WO2015005960A1 (en) * | 2013-07-12 | 2015-01-15 | Emd Millipore Corporation | Removal of fragments from a sample containing a target protein using activated carbon |
-
2012
- 2012-08-02 US US13/565,463 patent/US9096648B2/en active Active
- 2012-08-02 WO PCT/US2012/049351 patent/WO2013028330A2/en not_active Ceased
- 2012-08-07 TW TW101128407A patent/TWI574975B/zh active
- 2012-08-09 ES ES12179861.5T patent/ES2539232T3/es active Active
- 2012-08-09 ES ES15159525.3T patent/ES2677650T3/es active Active
- 2012-08-09 EP EP12179861.5A patent/EP2574618B1/en active Active
- 2012-08-09 EP EP15159525.3A patent/EP2942353B1/en active Active
- 2012-08-13 JP JP2012179197A patent/JP5938295B2/ja active Active
- 2012-08-16 KR KR20120089449A patent/KR101484659B1/ko active Active
- 2012-08-20 CN CN202010257856.2A patent/CN111423488B/zh active Active
- 2012-08-20 CN CN201210398359.XA patent/CN102977182B/zh active Active
- 2012-08-20 CN CN201610090272.4A patent/CN105669829B/zh active Active
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2014
- 2014-12-04 JP JP2014246058A patent/JP6254072B2/ja active Active
-
2015
- 2015-06-23 US US14/747,029 patent/US10287314B2/en active Active
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2016
- 2016-02-09 JP JP2016022509A patent/JP6339120B2/ja active Active
-
2018
- 2018-02-02 JP JP2018017028A patent/JP6743074B2/ja active Active
-
2019
- 2019-03-20 US US16/358,845 patent/US11634457B2/en active Active
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