JP2015014613A - 高感度分子検出及び分析を可能にする、ナノポアを有するベアの単層グラフェン膜 - Google Patents
高感度分子検出及び分析を可能にする、ナノポアを有するベアの単層グラフェン膜 Download PDFInfo
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Abstract
Description
本出願は、その全体が参照により本明細書に組み込まれる、2009年9月18日に提出された米国仮出願第61/243,607号の利益を主張する。本出願はまた、その全体が参照により本明細書に組み込まれる、2010年6月16日に提出された米国仮出願第61/355,528号の利益も主張する。
本出願は、NIHによって授与された契約番号2R01HG003703-04の下に政府の援助によってなされた。政府は、本出願においてある一定の権利を有する。
G薄い=σ・d (1)
(式中、σ=F(μK+μCl)Cは、イオン溶液の伝導率であり、Fは、ファラデー定数であり、cは、イオン濃度であり、μi(c)は、KClイオン溶液に用いられるカリウム(i=K)及び塩化物(i=Cl)イオンの移動度である)によって与える。コンダクタンスの直径への線形依存性は、先に記載されているように、無限に薄い膜についてはナノポアの周囲で鋭いピークとなる電流密度から得られる。ナノポア直径よりも厚い膜では、伝導率が、ナノポア面積に比例することとなる。有限であるが厚さが薄い膜では、コンピュータ計算により、コンダクタンスを予測することができる。
[1]
グラフェンナノポアセンサであって、
実質的にベアの単層グラフェン膜であって、第1のグラフェン膜表面から第1のグラフェン膜表面の対向側にある第2のグラフェン膜表面までグラフェン膜の厚さを通って延在するナノポアを含む、グラフェン膜と、
イオン溶液中の化学種を第1のグラフェン膜表面においてナノポアに提供するための、第1のグラフェン膜表面から第1のリザーバまでの接続部と、
第1のグラフェン膜表面から第2のグラフェン膜表面までナノポアを通って該化学種及びイオン溶液が転位した後に該化学種及びイオン溶液を収集するための、第2のグラフェン膜表面から第2のリザーバまでの接続部と、
ナノポアの対向する両側に接続されている、グラフェン膜におけるナノポアを通るイオン電流の流れを測定するための電気回路と
を含むグラフェンナノポアセンサ。
[2]
電気回路が、グラフェン膜におけるナノポアを通るイオン電流の流れを測定するために、第1のイオン溶液と第2のイオン溶液との間に接続されている、[1]に記載のグラフェンナノポアセンサ。
[3]
電気回路が、ナノポアを通る時間依存性イオン電流の流れを測定するために接続された電流モニターを含む、[1]に記載のグラフェンナノポアセンサ。
[4]
電流モニターが、ナノポアを通る化学種の転位を示す時間依存性イオン電流の流れの遮断を測定するために接続されている、[3]に記載のグラフェンナノポアセンサ。
[5]
ナノポアに電圧をかけて、ナノポアを通る化学種の転位を電気泳動的に引き起こすための、第1及び第2のイオン溶液のそれぞれに設けられた電極をさらに含む、[1]に記載のグラフェンナノポアセンサ。
[6]
イオン溶液が、約2Mを超える塩含量を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[7]
イオン溶液が、約8を超えるpHを特徴とする、[1]に記載のグラフェンナノポアセンサ。
[8]
イオン溶液がKClである、[1]に記載のグラフェンナノポアセンサ。
[9]
ナノポアが、ナノポアが延在するグラフェン膜の第1の表面と第2の表面との間のグラフェン膜厚より大きい直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[10]
ナノポアが、約1nmと約10nmとの間である直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[11]
ナノポアが、約1nmと約5nmとの間である直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[12]
ナノポアが、約3nm未満である直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[13]
ナノポアが、約2.5nm未満である直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[14]
グラフェン膜が、約2nm未満である厚さを特徴とする、[1]に記載のグラフェンナノポアセンサ。
[15]
グラフェン膜が、約1nm未満である厚さを特徴とする、[1]に記載のグラフェンナノポアセンサ。
[16]
グラフェン膜が、約0.7nm未満である厚さを特徴とする、[1]に記載のグラフェンナノポアセンサ。
[17]
ナノポアが、ナノポアを通って転位するイオン溶液中の化学種に特有の直径と比べ約5%以下だけ大きい直径を特徴とする、[1]に記載のグラフェンナノポアセンサ。
[18]
グラフェン膜が、膜の縁部において膜フレーム構造体によって機械的に支持されている、[1]に記載のグラフェンナノポアセンサ。
[19]
ナノポアを通って転位するイオン溶液中の化学種が、生体分子を含む、[1]に記載のグラフェンナノポアセンサ。
[20]
ナノポアを通って転位するイオン溶液中の化学種が、DNA分子を含む、[1]に記載のグラフェンナノポアセンサ。
[21]
ナノポアを通って転位するイオン溶液中の化学種が、RNA分子を含む、[1]に記載のグラフェンナノポアセンサ。
[22]
ナノポアを通って転位するイオン溶液中の化学種が、オリゴヌクレオチドを含む、[1]に記載のグラフェンナノポアセンサ。
[23]
ナノポアを通って転位するイオン溶液中の化学種が、ポリマー分子を含む、[1]に記載のグラフェンナノポアセンサ。
[24]
ナノポアを通って転位するイオン溶液中の化学種が、ヌクレオチドを含む、[1]に記載のグラフェンナノポアセンサ。
[25]
グラフェンナノポアセンサであって、
実質的にベアの単層グラフェン膜であって、第1のグラフェン膜表面から第1のグラフェン表面の対向側にある第2のグラフェン膜表面までグラフェン膜の厚さを通って延在し、約3nm未満であってグラフェン厚さを超える直径を有するナノポアを含むグラフェン膜と、
ナノポアの対向する両側に接続されている、グラフェン膜におけるナノポアを通るイオン電流の流れを測定するための電気回路と
を含むグラフェンナノポアセンサ。
[26]
イオン溶液中のポリマー分子を第1のグラフェン膜表面においてナノポアに提供するための、第1のグラフェン膜表面から第1のリザーバまでの接続部と、
第1のグラフェン膜表面から第2のグラフェン膜表面までナノポアを通ってポリマー分子及びイオン溶液が転位した後にポリマー分子及びイオン溶液を収集するための、第2のグラフェン膜表面から第2のリザーバまでの接続部と
をさらに含む、[25]に記載のグラフェンナノポアセンサ。
[27]
電流モニターが、ナノポアを通るポリマー分子の転位を示す時間依存性イオン電流の流れの遮断を測定するために接続されている、[25]に記載のグラフェンナノポアセンサ。
[28]
ナノポアに電圧をかけて、ナノポアの化学種の転位を電気泳動的に引き起こすための、第1及び第2のイオン溶液のそれぞれに設けられた電極をさらに含む、[26]に記載のグラフェンナノポアセンサ。
[29]
イオン溶液が、約2Mを超える塩含量及び約8を超えるpHを特徴とする、[25]に記載のグラフェンナノポアセンサ。
[30]
ナノポアセンサであって、
第1の膜表面と第1の膜表面の対向側にある第2の膜表面との間にある、約1nm未満である厚さを有する固体状態膜と、
第1及び第2の膜表面の間の膜厚を通して延在し、膜厚より大きい直径を有するナノポアと、
イオン溶液中の化学種を第1の膜表面においてナノポアに提供するための、第1の膜表面から第1のリザーバまでの接続部と、
第1の膜表面から第2の膜表面までナノポアを通って該化学種及びイオン溶液が転位した後に該化学種及びイオン溶液を収集するための、第2の膜表面から第2のリザーバまでの接続部と、
固体状態膜におけるナノポアを通るイオン溶液中の化学種の転位をモニタリングするために接続された電気回路と
を含む、ナノポアセンサ。
[31]
ナノポアが、約1nmと約10nmとの間である直径を特徴とする、[30]に記載のナノポアセンサ。
[32]
ナノポアが、約1nmと約5nmとの間である直径を特徴とする、[30]に記載のナノポアセンサ。
[33]
ナノポアが、約3nm未満である直径を特徴とする、[30]に記載のナノポアセンサ。
[34]
ナノポアが、約2.5nm未満である直径を特徴とする、[30]に記載のナノポアセンサ。
[35]
ナノポアが、ナノポアを通って転位するイオン溶液中の化学種に特有の直径と比べ約5%以下だけ大きい直径を特徴とする、[30]に記載のナノポアセンサ。
[36]
ポリマー分子を評価するための方法であって、
イオン溶液中に、評価すべきポリマー分子を提供することと、
イオン溶液中のポリマー分子を、実質的にベアの単層グラフェン膜におけるナノポアを通って、第1のグラフェン膜表面から第1のグラフェン表面の対向側にある第2のグラフェン膜表面まで転位させることと、
グラフェン膜におけるナノポアを通るイオン電流の流れをモニタリングすることと
を含む方法。
[37]
イオン電流の流れをモニタリングすることが、ナノポアを通るポリマー分子の転位を示す時間依存性イオン電流の流れの遮断を測定することを含む、[36]に記載の方法。
[38]
ナノポアに電圧をかけて、ポリマー分子をナノポアを通って電気泳動的に転位させることをさらに含む、[36]に記載の方法。
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KR101732207B1 (ko) | 2017-05-02 |
BR112012005888A2 (pt) | 2017-06-06 |
EP2470899A1 (en) | 2012-07-04 |
JP6054922B2 (ja) | 2016-12-27 |
CA2772789C (en) | 2018-10-30 |
EP3196645A1 (en) | 2017-07-26 |
EP2470899B1 (en) | 2017-03-15 |
JP2013505448A (ja) | 2013-02-14 |
US20120234679A1 (en) | 2012-09-20 |
CN102630304B (zh) | 2014-11-26 |
AU2010307229A1 (en) | 2012-04-12 |
EP3196645B1 (en) | 2019-06-19 |
AU2010307229B2 (en) | 2016-02-25 |
US20200158712A1 (en) | 2020-05-21 |
BR112012005888B1 (pt) | 2019-10-22 |
JP5612695B2 (ja) | 2014-10-22 |
CN102630304A (zh) | 2012-08-08 |
AU2018201010B2 (en) | 2020-03-12 |
US10564144B2 (en) | 2020-02-18 |
AU2016203366A1 (en) | 2016-06-16 |
AU2018201010A1 (en) | 2018-03-01 |
WO2011046706A1 (en) | 2011-04-21 |
KR20120069720A (ko) | 2012-06-28 |
CA2772789A1 (en) | 2011-04-21 |
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