JP2014534812A5 - - Google Patents
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- JP2014534812A5 JP2014534812A5 JP2014536328A JP2014536328A JP2014534812A5 JP 2014534812 A5 JP2014534812 A5 JP 2014534812A5 JP 2014536328 A JP2014536328 A JP 2014536328A JP 2014536328 A JP2014536328 A JP 2014536328A JP 2014534812 A5 JP2014534812 A5 JP 2014534812A5
- Authority
- JP
- Japan
- Prior art keywords
- target polynucleotide
- pore
- helicase
- characterizing
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000002157 polynucleotide Substances 0.000 claims 26
- 102000040430 polynucleotide Human genes 0.000 claims 26
- 108091033319 polynucleotide Proteins 0.000 claims 26
- 239000011148 porous material Substances 0.000 claims 23
- 108060004795 Methyltransferase Proteins 0.000 claims 18
- 238000000034 method Methods 0.000 claims 13
- 102100022536 Helicase POLQ-like Human genes 0.000 claims 10
- 101000899334 Homo sapiens Helicase POLQ-like Proteins 0.000 claims 10
- 238000005259 measurement Methods 0.000 claims 5
- 108010013381 Porins Proteins 0.000 claims 4
- 150000001413 amino acids Chemical class 0.000 claims 4
- 102000007739 porin activity proteins Human genes 0.000 claims 4
- 230000000694 effects Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 108091005703 transmembrane proteins Proteins 0.000 claims 3
- 102000035160 transmembrane proteins Human genes 0.000 claims 3
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 2
- 108010006464 Hemolysin Proteins Proteins 0.000 claims 2
- 108010014603 Leukocidins Proteins 0.000 claims 2
- 241000187480 Mycobacterium smegmatis Species 0.000 claims 2
- 101710203388 Outer membrane porin G Proteins 0.000 claims 2
- 238000012512 characterization method Methods 0.000 claims 2
- 239000012530 fluid Substances 0.000 claims 2
- 239000003228 hemolysin Substances 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 150000003839 salts Chemical class 0.000 claims 2
- 102000004895 Lipoproteins Human genes 0.000 claims 1
- 108090001030 Lipoproteins Proteins 0.000 claims 1
- 241000588653 Neisseria Species 0.000 claims 1
- 108700006385 OmpF Proteins 0.000 claims 1
- 108010073429 Type V Secretion Systems Proteins 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 230000005669 field effect Effects 0.000 claims 1
- 238000002847 impedance measurement Methods 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 108010014203 outer membrane phospholipase A Proteins 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161549998P | 2011-10-21 | 2011-10-21 | |
| US61/549,998 | 2011-10-21 | ||
| US201261599244P | 2012-02-15 | 2012-02-15 | |
| US61/599,244 | 2012-02-15 | ||
| PCT/GB2012/052579 WO2013057495A2 (en) | 2011-10-21 | 2012-10-18 | Enzyme method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014534812A JP2014534812A (ja) | 2014-12-25 |
| JP2014534812A5 true JP2014534812A5 (cg-RX-API-DMAC7.html) | 2015-12-10 |
| JP6226869B2 JP6226869B2 (ja) | 2017-11-08 |
Family
ID=47073477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014536328A Active JP6226869B2 (ja) | 2011-10-21 | 2012-10-18 | 酵素法 |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US9758823B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2987870B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6226869B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR20140090633A (cg-RX-API-DMAC7.html) |
| CN (1) | CN104039979B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2012324639B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112014009579B1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2852812A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013057495A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (143)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20100196203A1 (en) | 2007-02-20 | 2010-08-05 | Gurdial Singh Sanghera | Formation of Lipid Bilayers |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| CN104710519B (zh) | 2008-09-22 | 2021-05-28 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| BRPI1007214A8 (pt) | 2009-01-30 | 2022-12-20 | Oxford Nanopore Tech Ltd | Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla. |
| JP6480183B2 (ja) | 2011-05-27 | 2019-03-06 | オックスフォード ナノポール テクノロジーズ リミテッド | 結合方法 |
| CA2843136C (en) | 2011-07-25 | 2020-12-29 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| US20160162634A1 (en) | 2011-09-23 | 2016-06-09 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CN104136631B (zh) | 2011-12-29 | 2017-03-01 | 牛津纳米孔技术公司 | 使用xpd解旋酶表征多核苷酸的方法 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| JP6275052B2 (ja) | 2012-02-15 | 2018-02-07 | オックスフォード ナノポール テクノロジーズ リミテッド | アプタマー法 |
| CA2866587C (en) | 2012-02-16 | 2022-07-26 | Oxford Nanopore Technologies Limited | Analysis of measurements of a polymer |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| EP3741847A1 (en) | 2012-07-19 | 2020-11-25 | Oxford Nanopore Technologies Limited | Modified helicases |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| CA2879355C (en) | 2012-07-19 | 2021-09-21 | Oxford Nanopore Technologies Limited | Helicase construct and its use in characterising polynucleotides |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CN105209634B (zh) | 2013-03-08 | 2020-05-12 | 牛津纳米孔技术公司 | 酶停滞方法 |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| US9766248B2 (en) * | 2013-05-23 | 2017-09-19 | Arizona Board of Regents of behalf of Arizona State University | Chemistry, systems and methods of translocation of a polymer through a nanopore |
| CN105899678A (zh) | 2013-10-18 | 2016-08-24 | 牛津纳米孔技术公司 | 经修饰的酶 |
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| DK3074534T3 (da) * | 2013-11-26 | 2019-07-29 | Illumina Inc | Fremgangsmåder til polynukleotidsekventering |
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| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| SG11201607796TA (en) | 2014-02-19 | 2016-11-29 | Univ Washington | Nanopore-based analysis of protein characteristics |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| CN106460061B (zh) | 2014-04-04 | 2020-03-06 | 牛津纳米孔技术公司 | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
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| SG10201809900PA (en) * | 2014-07-31 | 2018-12-28 | Illumina Inc | Hybrid Nanopore Sensors |
| KR20250130441A (ko) | 2014-09-01 | 2025-09-01 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
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| CN115851894B (zh) | 2014-10-16 | 2025-06-27 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
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| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| GB201508003D0 (en) | 2015-05-11 | 2015-06-24 | Oxford Nanopore Tech Ltd | Apparatus and methods for measuring an electrical current |
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| KR102828319B1 (ko) | 2015-06-03 | 2025-07-02 | 일루미나, 인코포레이티드 | 나노기공에 인접한 중합효소에 고정된 테터를 이용하여 폴리뉴클레오타이드를 서열결정하기 위한 조성물, 시스템 및 방법 |
| JP7237586B2 (ja) | 2015-12-08 | 2023-03-13 | カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント | 修飾ナノポア、それを含む組成物およびその使用 |
| US11286517B2 (en) | 2016-02-17 | 2022-03-29 | President And Fellows Of Harvard College | Molecular programming tools |
| WO2017149318A1 (en) | 2016-03-02 | 2017-09-08 | Oxford Nanopore Technologies Limited | Mutant pores |
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| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
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| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| US11359229B2 (en) | 2016-09-20 | 2022-06-14 | President And Fellows Of Harvard College | Molecular verification systems |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| EP3568492B1 (en) | 2017-01-10 | 2025-01-01 | President and Fellows of Harvard College | Multiplexed signal amplification |
| CN109207454B (zh) | 2017-01-24 | 2022-02-15 | 广州孔确基因科技有限公司 | 蛋白、跨膜核酸解旋纳米孔及其构建方法与应用 |
| CN119530363A (zh) | 2017-02-10 | 2025-02-28 | 牛津纳米孔科技公开有限公司 | 修饰的纳米孔、包含其的组合物及其用途 |
| US11999948B2 (en) * | 2017-05-04 | 2024-06-04 | The Regents Of The University Of California | Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| KR102885764B1 (ko) | 2017-06-29 | 2025-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 나노포어를 통한 폴리머의 결정적 스텝핑 |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
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| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| CN111630179B (zh) | 2018-01-26 | 2024-07-19 | 哈佛学院院长及董事 | 邻近检测方法和组合物 |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| CN112189055B (zh) | 2018-03-22 | 2024-07-19 | 哈佛学院院长及董事 | 用于分子认证的方法和组合物 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
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| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| US20220162692A1 (en) | 2018-07-30 | 2022-05-26 | Oxford University Innovation Limited | Assemblies |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| WO2020061997A1 (zh) * | 2018-09-28 | 2020-04-02 | 北京齐碳科技有限公司 | 一种解旋酶及其应用 |
| WO2020084705A1 (ja) * | 2018-10-24 | 2020-04-30 | 株式会社日立ハイテク | 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法 |
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| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| CN121090823A (zh) | 2019-03-12 | 2025-12-09 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置、组件和操作方法 |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
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| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| CN114502714A (zh) * | 2019-07-31 | 2022-05-13 | 安序源有限公司 | 评估靶分子的系统和方法 |
| WO2021056599A1 (zh) | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| KR20220110213A (ko) | 2019-12-02 | 2022-08-05 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어를 사용하여 표적 폴리펩티드를 특징분석하는 방법 |
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| WO2021124468A1 (ja) * | 2019-12-18 | 2021-06-24 | 株式会社日立ハイテク | 分子複合体、および生体ポリマ分析方法 |
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| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| CN115667542A (zh) | 2020-06-10 | 2023-01-31 | 豪夫迈·罗氏有限公司 | 用于膜中自限性蛋白质孔插入的法拉第系统和方法 |
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