JP2017532962A5 - - Google Patents
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- Publication number
- JP2017532962A5 JP2017532962A5 JP2017520492A JP2017520492A JP2017532962A5 JP 2017532962 A5 JP2017532962 A5 JP 2017532962A5 JP 2017520492 A JP2017520492 A JP 2017520492A JP 2017520492 A JP2017520492 A JP 2017520492A JP 2017532962 A5 JP2017532962 A5 JP 2017532962A5
- Authority
- JP
- Japan
- Prior art keywords
- rna polynucleotide
- helicase
- dna
- polynucleotide
- leader sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 12
- 108060004795 Methyltransferase Proteins 0.000 claims 11
- 108090000133 DNA helicases Proteins 0.000 claims 10
- 102000003844 DNA helicases Human genes 0.000 claims 10
- 239000011148 porous material Substances 0.000 claims 10
- 102000040430 polynucleotide Human genes 0.000 claims 5
- 108091033319 polynucleotide Proteins 0.000 claims 5
- 239000002157 polynucleotide Substances 0.000 claims 5
- 238000005259 measurement Methods 0.000 claims 4
- 241000588653 Neisseria Species 0.000 claims 2
- 101710203389 Outer membrane porin F Proteins 0.000 claims 2
- 101710203388 Outer membrane porin G Proteins 0.000 claims 2
- 230000004048 modification Effects 0.000 claims 2
- 238000012986 modification Methods 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 102100022536 Helicase POLQ-like Human genes 0.000 claims 1
- 108010006464 Hemolysin Proteins Proteins 0.000 claims 1
- 101000899334 Homo sapiens Helicase POLQ-like Proteins 0.000 claims 1
- 108010014603 Leukocidins Proteins 0.000 claims 1
- 102000004895 Lipoproteins Human genes 0.000 claims 1
- 108090001030 Lipoproteins Proteins 0.000 claims 1
- 241000187480 Mycobacterium smegmatis Species 0.000 claims 1
- 108010013381 Porins Proteins 0.000 claims 1
- 108010073429 Type V Secretion Systems Proteins 0.000 claims 1
- 238000012512 characterization method Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000003228 hemolysin Substances 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 230000011987 methylation Effects 0.000 claims 1
- 238000007069 methylation reaction Methods 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 108010014203 outer membrane phospholipase A Proteins 0.000 claims 1
- 230000003647 oxidation Effects 0.000 claims 1
- 238000007254 oxidation reaction Methods 0.000 claims 1
- 102000007739 porin activity proteins Human genes 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1418459.2 | 2014-10-17 | ||
| GB201418459A GB201418459D0 (en) | 2014-10-17 | 2014-10-17 | Method |
| GB1508270.4 | 2015-05-14 | ||
| GBGB1508270.4A GB201508270D0 (en) | 2015-05-14 | 2015-05-14 | Method |
| GBPCT/GB2015/052916 | 2015-10-06 | ||
| GB1517634.0 | 2015-10-06 | ||
| PCT/GB2015/052916 WO2016055777A2 (en) | 2014-10-07 | 2015-10-06 | Modified enzymes |
| GBGB1517634.0A GB201517634D0 (en) | 2015-10-06 | 2015-10-06 | Method |
| PCT/GB2015/053097 WO2016059436A1 (en) | 2014-10-17 | 2015-10-19 | Method for nanopore rna characterisation |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017532962A JP2017532962A (ja) | 2017-11-09 |
| JP2017532962A5 true JP2017532962A5 (cg-RX-API-DMAC7.html) | 2018-11-29 |
| JP6824881B2 JP6824881B2 (ja) | 2021-02-03 |
Family
ID=54337815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017520492A Active JP6824881B2 (ja) | 2014-10-17 | 2015-10-19 | ナノ細孔rnaを特徴付けるための方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US10480026B2 (cg-RX-API-DMAC7.html) |
| EP (1) | EP3207155B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6824881B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102457147B1 (cg-RX-API-DMAC7.html) |
| CN (2) | CN113981055A (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016059436A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| JP6275052B2 (ja) | 2012-02-15 | 2018-02-07 | オックスフォード ナノポール テクノロジーズ リミテッド | アプタマー法 |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| US9116118B2 (en) | 2012-06-08 | 2015-08-25 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| EP3741847A1 (en) | 2012-07-19 | 2020-11-25 | Oxford Nanopore Technologies Limited | Modified helicases |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| CN105899678A (zh) | 2013-10-18 | 2016-08-24 | 牛津纳米孔技术公司 | 经修饰的酶 |
| GB201417712D0 (en) * | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| KR20250130441A (ko) | 2014-09-01 | 2025-09-01 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| CN113981055A (zh) | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| WO2017149318A1 (en) | 2016-03-02 | 2017-09-08 | Oxford Nanopore Technologies Limited | Mutant pores |
| US11514289B1 (en) | 2016-03-09 | 2022-11-29 | Freenome Holdings, Inc. | Generating machine learning models using genetic data |
| EP4122949B1 (en) | 2016-04-06 | 2024-06-05 | Oxford Nanopore Technologies plc | Mutant pore |
| EP3464616B1 (en) * | 2016-05-25 | 2022-05-04 | Oxford Nanopore Technologies plc | Method |
| GB201609221D0 (en) * | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201617886D0 (en) * | 2016-10-21 | 2016-12-07 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| WO2018200847A1 (en) * | 2017-04-27 | 2018-11-01 | The Regents Of The University Of California | Methods of producing ribosomal ribonucleic acid complexes |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| KR102885764B1 (ko) * | 2017-06-29 | 2025-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 나노포어를 통한 폴리머의 결정적 스텝핑 |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2569977A (en) * | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| WO2019149626A1 (de) | 2018-02-02 | 2019-08-08 | Bayer Aktiengesellschaft | Bekämpfung resistenter schadorganismen |
| WO2019226822A1 (en) * | 2018-05-23 | 2019-11-28 | The Regents Of The University Of California | Methods of analyzing capped ribonucleic acids |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
| EP3650559B1 (en) * | 2018-11-08 | 2022-06-29 | Siemens Healthcare GmbH | Direct rna nanopore sequencing with help of a stem-loop polynucleotide |
| CN111455031A (zh) * | 2019-01-18 | 2020-07-28 | 中国科学院微生物研究所 | 基于Nanopore测序技术的多组学测序及分析方法 |
| CN110093399A (zh) * | 2019-04-22 | 2019-08-06 | 武汉大学 | 一种利用二磷酸尿嘧啶脱氧核苷酸检测核酸中腺嘌呤n6位发生甲基化修饰的方法 |
| GB201907244D0 (en) * | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| WO2021036995A1 (en) * | 2019-08-23 | 2021-03-04 | Nanjing University | Direct microrna sequencing using enzyme assisted sequencing |
| CN110568039B (zh) * | 2019-09-06 | 2021-05-04 | 中国科学院生物物理研究所 | 使用新型电化学传感器的氨基酸特异实时检测方法 |
| CN111164097B (zh) * | 2019-09-29 | 2021-02-19 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| CN113930406B (zh) * | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| GB202107192D0 (en) * | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| CN114134142B (zh) * | 2021-11-23 | 2024-05-28 | 成都齐碳科技有限公司 | 衔接体、复合物、单链分子、试剂盒、方法和应用 |
| CN116497102A (zh) * | 2021-12-21 | 2023-07-28 | 成都齐碳科技有限公司 | 用于表征目标多核苷酸的衔接体、方法及其用途 |
| GB202204919D0 (en) | 2022-04-04 | 2022-05-18 | Oxford Nanopore Tech Plc | Method |
| WO2023225988A1 (zh) * | 2022-05-27 | 2023-11-30 | 深圳华大生命科学研究院 | 一种维持纳米孔测序速度的方法 |
| EP4616402A1 (en) | 2022-11-10 | 2025-09-17 | Bayer Aktiengesellschaft | Targeted crop protection product application based on genetic profiles |
| CN116478983B (zh) * | 2022-11-21 | 2023-10-24 | 北京普译生物科技有限公司 | 一种rna-dna嵌合接头及其应用 |
| CN116676175B (zh) * | 2023-03-17 | 2024-04-09 | 四川大学 | 一种多条形码直接rna纳米孔测序分类器 |
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| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
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| EP3741847A1 (en) * | 2012-07-19 | 2020-11-25 | Oxford Nanopore Technologies Limited | Modified helicases |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| CN105209634B (zh) | 2013-03-08 | 2020-05-12 | 牛津纳米孔技术公司 | 酶停滞方法 |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| CN105899678A (zh) | 2013-10-18 | 2016-08-24 | 牛津纳米孔技术公司 | 经修饰的酶 |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN106460061B (zh) | 2014-04-04 | 2020-03-06 | 牛津纳米孔技术公司 | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 |
| CN113981055A (zh) | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
-
2015
- 2015-10-19 CN CN202111321280.2A patent/CN113981055A/zh active Pending
- 2015-10-19 JP JP2017520492A patent/JP6824881B2/ja active Active
- 2015-10-19 CN CN201580069027.9A patent/CN107109489B/zh active Active
- 2015-10-19 US US15/519,599 patent/US10480026B2/en active Active
- 2015-10-19 KR KR1020177013136A patent/KR102457147B1/ko active Active
- 2015-10-19 EP EP15784137.0A patent/EP3207155B1/en active Active
- 2015-10-19 WO PCT/GB2015/053097 patent/WO2016059436A1/en not_active Ceased
-
2019
- 2019-09-19 US US16/575,610 patent/US11021747B2/en active Active
-
2021
- 2021-04-30 US US17/246,462 patent/US12129518B2/en active Active
-
2024
- 2024-09-19 US US18/889,917 patent/US20250084472A1/en active Pending
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