AU2012324639B2 - Method of characterizing a target polynucleotide using a pore and a Hel308 helicase - Google Patents
Method of characterizing a target polynucleotide using a pore and a Hel308 helicase Download PDFInfo
- Publication number
- AU2012324639B2 AU2012324639B2 AU2012324639A AU2012324639A AU2012324639B2 AU 2012324639 B2 AU2012324639 B2 AU 2012324639B2 AU 2012324639 A AU2012324639 A AU 2012324639A AU 2012324639 A AU2012324639 A AU 2012324639A AU 2012324639 B2 AU2012324639 B2 AU 2012324639B2
- Authority
- AU
- Australia
- Prior art keywords
- pore
- target polynucleotide
- helicase
- seq
- hel308
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/513—Winding/unwinding enzyme, e.g. helicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161549998P | 2011-10-21 | 2011-10-21 | |
| US61/549,998 | 2011-10-21 | ||
| US201261599244P | 2012-02-15 | 2012-02-15 | |
| US61/599,244 | 2012-02-15 | ||
| PCT/GB2012/052579 WO2013057495A2 (en) | 2011-10-21 | 2012-10-18 | Enzyme method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2012324639A1 AU2012324639A1 (en) | 2014-05-22 |
| AU2012324639B2 true AU2012324639B2 (en) | 2017-11-16 |
Family
ID=47073477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2012324639A Active AU2012324639B2 (en) | 2011-10-21 | 2012-10-18 | Method of characterizing a target polynucleotide using a pore and a Hel308 helicase |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US9758823B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP2987870B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6226869B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR20140090633A (cg-RX-API-DMAC7.html) |
| CN (1) | CN104039979B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2012324639B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112014009579B1 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2852812A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2013057495A2 (cg-RX-API-DMAC7.html) |
Families Citing this family (143)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100196203A1 (en) | 2007-02-20 | 2010-08-05 | Gurdial Singh Sanghera | Formation of Lipid Bilayers |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| CN104710519B (zh) | 2008-09-22 | 2021-05-28 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| BRPI1007214A8 (pt) | 2009-01-30 | 2022-12-20 | Oxford Nanopore Tech Ltd | Adaptador para sequenciar ácidos nucleicos, par de adaptadores, kit, construto de ácido nucleico, e, métodos para preparar um adaptador, um construto de ácido nucleico, e um construto de dequência, e para sequenciar ácido nucleico de fita dupla. |
| JP6480183B2 (ja) | 2011-05-27 | 2019-03-06 | オックスフォード ナノポール テクノロジーズ リミテッド | 結合方法 |
| CA2843136C (en) | 2011-07-25 | 2020-12-29 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| US20160162634A1 (en) | 2011-09-23 | 2016-06-09 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CN104136631B (zh) | 2011-12-29 | 2017-03-01 | 牛津纳米孔技术公司 | 使用xpd解旋酶表征多核苷酸的方法 |
| GB201202519D0 (en) | 2012-02-13 | 2012-03-28 | Oxford Nanopore Tech Ltd | Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules |
| JP6275052B2 (ja) | 2012-02-15 | 2018-02-07 | オックスフォード ナノポール テクノロジーズ リミテッド | アプタマー法 |
| CA2866587C (en) | 2012-02-16 | 2022-07-26 | Oxford Nanopore Technologies Limited | Analysis of measurements of a polymer |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| EP3741847A1 (en) | 2012-07-19 | 2020-11-25 | Oxford Nanopore Technologies Limited | Modified helicases |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| CA2879355C (en) | 2012-07-19 | 2021-09-21 | Oxford Nanopore Technologies Limited | Helicase construct and its use in characterising polynucleotides |
| EP2895618B1 (en) | 2012-09-14 | 2017-07-26 | Oxford Nanopore Technologies Limited | Sample preparation method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| EP2917366B1 (en) | 2012-11-06 | 2017-08-02 | Oxford Nanopore Technologies Limited | Quadruplex method |
| GB201222928D0 (en) | 2012-12-19 | 2013-01-30 | Oxford Nanopore Tech Ltd | Analysis of a polynucleotide |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| CN105209634B (zh) | 2013-03-08 | 2020-05-12 | 牛津纳米孔技术公司 | 酶停滞方法 |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| US9766248B2 (en) * | 2013-05-23 | 2017-09-19 | Arizona Board of Regents of behalf of Arizona State University | Chemistry, systems and methods of translocation of a polymer through a nanopore |
| CN105899678A (zh) | 2013-10-18 | 2016-08-24 | 牛津纳米孔技术公司 | 经修饰的酶 |
| GB201406151D0 (en) * | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| DK3074534T3 (da) * | 2013-11-26 | 2019-07-29 | Illumina Inc | Fremgangsmåder til polynukleotidsekventering |
| AU2015208919B9 (en) | 2014-01-22 | 2021-04-01 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201406155D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| SG11201607796TA (en) | 2014-02-19 | 2016-11-29 | Univ Washington | Nanopore-based analysis of protein characteristics |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| CN106460061B (zh) | 2014-04-04 | 2020-03-06 | 牛津纳米孔技术公司 | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
| AU2015271824B2 (en) * | 2014-06-03 | 2020-12-03 | Illumina, Inc. | Compositions, systems, and methods for detecting events using tethers anchored to or adjacent to nanopores |
| GB201411285D0 (en) * | 2014-06-25 | 2014-08-06 | Prosser Joseph | Sequencer |
| SG10201809900PA (en) * | 2014-07-31 | 2018-12-28 | Illumina Inc | Hybrid Nanopore Sensors |
| KR20250130441A (ko) | 2014-09-01 | 2025-09-01 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| WO2016055778A1 (en) | 2014-10-07 | 2016-04-14 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| CN115851894B (zh) | 2014-10-16 | 2025-06-27 | 牛津楠路珀尔科技股份有限公司 | 聚合物的分析 |
| GB201418469D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Method |
| GB201418512D0 (en) | 2014-10-17 | 2014-12-03 | Oxford Nanopore Tech Ltd | Electrical device with detachable components |
| CN113981055A (zh) * | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| US11639522B2 (en) | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| CN107533045B (zh) | 2015-02-05 | 2020-10-09 | 哈佛大学校长及研究员协会 | 包括流体通道的纳米孔感测器 |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| GB201502809D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Mutant pore |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| GB201508003D0 (en) | 2015-05-11 | 2015-06-24 | Oxford Nanopore Tech Ltd | Apparatus and methods for measuring an electrical current |
| GB201508669D0 (en) | 2015-05-20 | 2015-07-01 | Oxford Nanopore Tech Ltd | Methods and apparatus for forming apertures in a solid state membrane using dielectric breakdown |
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| JP7237586B2 (ja) | 2015-12-08 | 2023-03-13 | カトリック ユニヴェルシテット ルーヴェン カーユー ルーヴェン リサーチ アンド ディベロップメント | 修飾ナノポア、それを含む組成物およびその使用 |
| US11286517B2 (en) | 2016-02-17 | 2022-03-29 | President And Fellows Of Harvard College | Molecular programming tools |
| WO2017149318A1 (en) | 2016-03-02 | 2017-09-08 | Oxford Nanopore Technologies Limited | Mutant pores |
| EP4122949B1 (en) | 2016-04-06 | 2024-06-05 | Oxford Nanopore Technologies plc | Mutant pore |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| GB201609221D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| EP3464616B1 (en) | 2016-05-25 | 2022-05-04 | Oxford Nanopore Technologies plc | Method |
| GB201611770D0 (en) | 2016-07-06 | 2016-08-17 | Oxford Nanopore Tech | Microfluidic device |
| US11359229B2 (en) | 2016-09-20 | 2022-06-14 | President And Fellows Of Harvard College | Molecular verification systems |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201619930D0 (en) | 2016-11-24 | 2017-01-11 | Oxford Nanopore Tech | Apparatus and methods for controlling insertion of a membrane channel into a membrane |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| EP3568492B1 (en) | 2017-01-10 | 2025-01-01 | President and Fellows of Harvard College | Multiplexed signal amplification |
| CN109207454B (zh) | 2017-01-24 | 2022-02-15 | 广州孔确基因科技有限公司 | 蛋白、跨膜核酸解旋纳米孔及其构建方法与应用 |
| CN119530363A (zh) | 2017-02-10 | 2025-02-28 | 牛津纳米孔科技公开有限公司 | 修饰的纳米孔、包含其的组合物及其用途 |
| US11999948B2 (en) * | 2017-05-04 | 2024-06-04 | The Regents Of The University Of California | Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| GB201707140D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| KR102885764B1 (ko) | 2017-06-29 | 2025-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 나노포어를 통한 폴리머의 결정적 스텝핑 |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB2568895B (en) | 2017-11-29 | 2021-10-27 | Oxford Nanopore Tech Ltd | Microfluidic device |
| GB2569977A (en) | 2018-01-05 | 2019-07-10 | Oxford Nanopore Tech Ltd | Method |
| CN111630179B (zh) | 2018-01-26 | 2024-07-19 | 哈佛学院院长及董事 | 邻近检测方法和组合物 |
| GB201801768D0 (en) | 2018-02-02 | 2018-03-21 | Oxford Nanopore Tech Ltd | Synthesis method |
| CN112189055B (zh) | 2018-03-22 | 2024-07-19 | 哈佛学院院长及董事 | 用于分子认证的方法和组合物 |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| GB201808556D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201808554D0 (en) | 2018-05-24 | 2018-07-11 | Oxford Nanopore Tech Ltd | Method |
| GB201809323D0 (en) | 2018-06-06 | 2018-07-25 | Oxford Nanopore Tech Ltd | Method |
| US20220162692A1 (en) | 2018-07-30 | 2022-05-26 | Oxford University Innovation Limited | Assemblies |
| JP2020031557A (ja) * | 2018-08-28 | 2020-03-05 | 株式会社日立ハイテクノロジーズ | 生体分子分析装置 |
| WO2020061997A1 (zh) * | 2018-09-28 | 2020-04-02 | 北京齐碳科技有限公司 | 一种解旋酶及其应用 |
| WO2020084705A1 (ja) * | 2018-10-24 | 2020-04-30 | 株式会社日立ハイテク | 生体ポリマ分析デバイス及びそれを用いた分析装置、並びに分析方法 |
| EP3650558A1 (en) * | 2018-11-07 | 2020-05-13 | Siemens Healthcare GmbH | Liquid sample workflow for nanopore sequencing |
| GB201821155D0 (en) | 2018-12-21 | 2019-02-06 | Oxford Nanopore Tech Ltd | Method |
| CN121090823A (zh) | 2019-03-12 | 2025-12-09 | 牛津纳米孔科技公开有限公司 | 纳米孔感测装置、组件和操作方法 |
| GB201907244D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| GB201907246D0 (en) | 2019-05-22 | 2019-07-03 | Oxford Nanopore Tech Ltd | Method |
| US11926819B2 (en) | 2019-05-28 | 2024-03-12 | The Regents Of The University Of California | Methods of adding polymers to ribonucleic acids |
| CN114502714A (zh) * | 2019-07-31 | 2022-05-13 | 安序源有限公司 | 评估靶分子的系统和方法 |
| WO2021056599A1 (zh) | 2019-09-29 | 2021-04-01 | 北京齐碳科技有限公司 | 一种Mnep单体变体及其应用 |
| GB201917060D0 (en) | 2019-11-22 | 2020-01-08 | Oxford Nanopore Tech Ltd | Method |
| KR20220110213A (ko) | 2019-12-02 | 2022-08-05 | 옥스포드 나노포어 테크놀로지즈 피엘씨 | 나노포어를 사용하여 표적 폴리펩티드를 특징분석하는 방법 |
| GB201917742D0 (en) | 2019-12-04 | 2020-01-15 | Oxford Nanopore Tech Ltd | Method |
| WO2021124468A1 (ja) * | 2019-12-18 | 2021-06-24 | 株式会社日立ハイテク | 分子複合体、および生体ポリマ分析方法 |
| NL2024579B1 (en) * | 2019-12-24 | 2021-09-06 | Univ Delft Tech | Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes |
| GB202004944D0 (en) | 2020-04-03 | 2020-05-20 | King S College London | Method |
| CN115667542A (zh) | 2020-06-10 | 2023-01-31 | 豪夫迈·罗氏有限公司 | 用于膜中自限性蛋白质孔插入的法拉第系统和方法 |
| GB202009349D0 (en) | 2020-06-18 | 2020-08-05 | Oxford Nanopore Tech Ltd | Method |
| WO2021255476A2 (en) | 2020-06-18 | 2021-12-23 | Oxford Nanopore Technologies Limited | Method |
| US20230295712A1 (en) | 2020-06-18 | 2023-09-21 | Oxford Nanopore Technologies Plc | A method of selectively characterising a polynucleotide using a detector |
| CN113930406B (zh) | 2021-12-17 | 2022-04-08 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| WO2021253410A1 (zh) | 2020-06-19 | 2021-12-23 | 北京齐碳科技有限公司 | 一种Pif1-like解旋酶及其应用 |
| WO2022013551A1 (en) | 2020-07-17 | 2022-01-20 | Oxford Nanopore Technologies Limited | Nanopore sensing device |
| EP4185865A1 (en) | 2020-07-22 | 2023-05-31 | Oxford Nanopore Technologies PLC | Solid state nanopore formation |
| GB202015993D0 (en) | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| WO2022126304A1 (zh) * | 2020-12-14 | 2022-06-23 | 北京齐碳科技有限公司 | 一种经修饰的解旋酶及其应用 |
| WO2022213253A1 (zh) | 2021-04-06 | 2022-10-13 | 成都齐碳科技有限公司 | 经修饰的Prp43解旋酶及其用途 |
| US20240240248A1 (en) | 2021-05-19 | 2024-07-18 | Oxford Nanopore Technologies Plc | Methods for complement strand sequencing |
| GB202107192D0 (en) | 2021-05-19 | 2021-06-30 | Oxford Nanopore Tech Ltd | Method |
| GB202107354D0 (en) | 2021-05-24 | 2021-07-07 | Oxford Nanopore Tech Ltd | Method |
| GB202112235D0 (en) | 2021-08-26 | 2021-10-13 | Oxford Nanopore Tech Ltd | Nanopore |
| GB202118906D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202118908D0 (en) | 2021-12-23 | 2022-02-09 | Oxford Nanopore Tech Ltd | Method |
| GB202205617D0 (en) | 2022-04-14 | 2022-06-01 | Oxford Nanopore Tech Plc | Novel modified protein pores and enzymes |
| WO2023215603A1 (en) | 2022-05-06 | 2023-11-09 | 10X Genomics, Inc. | Methods and compositions for in situ analysis of v(d)j sequences |
| CN119173641A (zh) | 2022-05-17 | 2024-12-20 | 牛津纳米孔科技公开有限公司 | 方法和衔接子 |
| WO2023225988A1 (zh) * | 2022-05-27 | 2023-11-30 | 深圳华大生命科学研究院 | 一种维持纳米孔测序速度的方法 |
| EP4569330A1 (en) | 2022-08-09 | 2025-06-18 | Oxford Nanopore Technologies PLC | Novel pore monomers and pores |
| GB202211607D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202211602D0 (en) | 2022-08-09 | 2022-09-21 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| WO2024089270A2 (en) | 2022-10-28 | 2024-05-02 | Oxford Nanopore Technologies Plc | Pore monomers and pores |
| GB202216162D0 (en) | 2022-10-31 | 2022-12-14 | Oxford Nanopore Tech Plc | Method |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| EP4636094A1 (en) * | 2022-12-16 | 2025-10-22 | BGI Shenzhen | Nanopore sequencing method and kit |
| GB202304324D0 (en) | 2023-03-24 | 2023-05-10 | Oxford Nanopore Tech Plc | Method and kits |
| GB202305524D0 (en) | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202307141D0 (en) | 2023-05-12 | 2023-06-28 | Oxford Nanopore Tech Plc | Modified helicases |
| GB202307486D0 (en) | 2023-05-18 | 2023-07-05 | Oxford Nanopore Tech Plc | Method |
| GB202315935D0 (en) | 2023-10-18 | 2023-11-29 | Oxford Nanopore Tech Ltd | Novel enzymes |
| GB202317028D0 (en) | 2023-11-06 | 2023-12-20 | Oxford Nanopore Tech Ltd | Method |
| WO2025120150A1 (en) | 2023-12-08 | 2025-06-12 | Oxford Nanopore Technologies Plc | Methods and apparatus for forming apertures, methods and apparatus for unblocking apertures, methods of sensing molecular entities in apertures, and measurement systems |
| GB202405960D0 (en) | 2024-04-29 | 2024-06-12 | Oxford Nanopore Tech Plc | Photo-active conjugates and their uses |
| GB202407100D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407055D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Enhanced polynucleotide sequencing |
| GB202407099D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | A flow cell and a method |
| GB202407044D0 (en) | 2024-05-17 | 2024-07-03 | Oxford Nanopore Tech Plc | Polymer analysis using transformer neural network |
| GB202407247D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
| GB202407228D0 (en) | 2024-05-21 | 2024-07-03 | Oxford Nanopore Tech Plc | Method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010004265A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| WO2010086622A1 (en) * | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
Family Cites Families (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6602979B1 (en) | 1994-12-19 | 2003-08-05 | The United States Of America As Represented By The Department Of Health And Human Services | Screening assays for compounds that cause apoptosis |
| US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
| WO2002092821A1 (en) | 2001-05-01 | 2002-11-21 | National Institute Of Advanced Industrial Science And Technology | Novel maxizyme |
| WO2002103210A1 (en) | 2001-06-15 | 2002-12-27 | Hansford Derek J | Nanopump devices and methods |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| DE60324810D1 (de) | 2002-09-20 | 2009-01-02 | New England Biolabs Inc | HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN |
| US7745116B2 (en) | 2003-04-08 | 2010-06-29 | Pacific Biosciences Of California, Inc. | Composition and method for nucleic acid sequencing |
| US7851203B2 (en) | 2003-10-01 | 2010-12-14 | Lawrence Livermore National Security, Llc | Functionalized apertures for the detection of chemical and biological materials |
| US7238485B2 (en) | 2004-03-23 | 2007-07-03 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
| WO2005124888A1 (en) | 2004-06-08 | 2005-12-29 | President And Fellows Of Harvard College | Suspended carbon nanotube field effect transistor |
| JP4757804B2 (ja) | 2004-10-27 | 2011-08-24 | 株式会社カネカ | 新規カルボニル還元酵素、その遺伝子、およびその利用法 |
| GB0505971D0 (en) | 2005-03-23 | 2005-04-27 | Isis Innovation | Delivery of molecules to a lipid bilayer |
| US20090269744A1 (en) | 2005-10-28 | 2009-10-29 | Exonhit Therapeutics Sa | Cancer detection method |
| GB0523282D0 (en) | 2005-11-15 | 2005-12-21 | Isis Innovation | Methods using pores |
| US20080010637A1 (en) | 2006-07-10 | 2008-01-10 | Silverbrook Research Pty Ltd | Pictbridge printer firmware upgrades via camera |
| US20100196203A1 (en) | 2007-02-20 | 2010-08-05 | Gurdial Singh Sanghera | Formation of Lipid Bilayers |
| EP2156179B1 (en) | 2007-04-04 | 2021-08-18 | The Regents of The University of California | Methods for using a nanopore |
| EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
| EP3540436B1 (en) | 2007-09-12 | 2023-11-01 | President And Fellows Of Harvard College | High-resolution molecular sensor |
| GB2453377A (en) | 2007-10-05 | 2009-04-08 | Isis Innovation | Transmembrane protein pores and molecular adapters therefore. |
| GB0724736D0 (en) | 2007-12-19 | 2008-01-30 | Oxford Nanolabs Ltd | Formation of layers of amphiphilic molecules |
| US8231969B2 (en) | 2008-03-26 | 2012-07-31 | University Of Utah Research Foundation | Asymmetrically functionalized nanoparticles |
| CN102144037A (zh) | 2008-07-07 | 2011-08-03 | 牛津纳米孔技术有限公司 | 检测碱基的孔 |
| US20100092960A1 (en) | 2008-07-25 | 2010-04-15 | Pacific Biosciences Of California, Inc. | Helicase-assisted sequencing with molecular beacons |
| CN104710519B (zh) * | 2008-09-22 | 2021-05-28 | 华盛顿大学 | Msp纳米微孔和相关方法 |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| GB0820927D0 (en) | 2008-11-14 | 2008-12-24 | Isis Innovation | Method |
| AU2010209508C1 (en) | 2009-01-30 | 2017-10-19 | Oxford Nanopore Technologies Limited | Hybridization linkers |
| CN102481341B (zh) | 2009-02-23 | 2017-05-17 | 希托马克斯医疗有限公司 | 蛋白原及其使用方法 |
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| US8986928B2 (en) | 2009-04-10 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Nanopore sequencing devices and methods |
| CN102405410B (zh) | 2009-04-20 | 2014-06-25 | 牛津楠路珀尔科技有限公司 | 脂质双层传感器阵列 |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| EP2507387B1 (en) | 2009-12-01 | 2017-01-25 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument and method |
| WO2011151941A1 (ja) | 2010-06-04 | 2011-12-08 | 国立大学法人東京大学 | 制御性ｔ細胞の増殖または集積を誘導する作用を有する組成物 |
| CN103154729B (zh) | 2010-06-08 | 2015-01-07 | 哈佛大学校长及研究员协会 | 具有由石墨烯支持的人工脂质膜的纳米孔装置 |
| WO2012033524A2 (en) | 2010-09-07 | 2012-03-15 | The Regents Of The University Of California | Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme |
| BR112013020411B1 (pt) | 2011-02-11 | 2021-09-08 | Oxford Nanopore Technologies Limited | Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo |
| US9347929B2 (en) | 2011-03-01 | 2016-05-24 | The Regents Of The University Of Michigan | Controlling translocation through nanopores with fluid wall |
| JP6480183B2 (ja) | 2011-05-27 | 2019-03-06 | オックスフォード ナノポール テクノロジーズ リミテッド | 結合方法 |
| CA2843136C (en) | 2011-07-25 | 2020-12-29 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| US20160162634A1 (en) | 2011-09-23 | 2016-06-09 | Oxford Nanopore Technologies Limited | Analysis of a polymer comprising polymer units |
| CN104039979B (zh) | 2011-10-21 | 2016-08-24 | 牛津纳米孔技术公司 | 使用孔和Hel308解旋酶表征目标多核苷酸的酶方法 |
| CN104136631B (zh) | 2011-12-29 | 2017-03-01 | 牛津纳米孔技术公司 | 使用xpd解旋酶表征多核苷酸的方法 |
| AU2012360244B2 (en) | 2011-12-29 | 2018-08-23 | Oxford Nanopore Technologies Limited | Enzyme method |
| CN112646019B (zh) | 2012-04-10 | 2022-08-16 | 牛津纳米孔科技公开有限公司 | 突变胞溶素孔 |
| US9116118B2 (en) | 2012-06-08 | 2015-08-25 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
| CA2879355C (en) | 2012-07-19 | 2021-09-21 | Oxford Nanopore Technologies Limited | Helicase construct and its use in characterising polynucleotides |
| EP3741847A1 (en) | 2012-07-19 | 2020-11-25 | Oxford Nanopore Technologies Limited | Modified helicases |
| WO2014013259A1 (en) | 2012-07-19 | 2014-01-23 | Oxford Nanopore Technologies Limited | Ssb method |
| GB201313121D0 (en) | 2013-07-23 | 2013-09-04 | Oxford Nanopore Tech Ltd | Array of volumes of polar medium |
| WO2014064444A1 (en) | 2012-10-26 | 2014-05-01 | Oxford Nanopore Technologies Limited | Droplet interfaces |
| GB201318465D0 (en) | 2013-10-18 | 2013-12-04 | Oxford Nanopore Tech Ltd | Method |
| CN105209634B (zh) | 2013-03-08 | 2020-05-12 | 牛津纳米孔技术公司 | 酶停滞方法 |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| RU2669844C2 (ru) | 2013-03-14 | 2018-10-16 | Аркема Инк. | Способы сшивания полимерных композиций в присутствии атмосферного кислорода |
| PT3004378T (pt) | 2013-05-24 | 2018-03-06 | Illumina Cambridge Ltd | Resumo |
| GB201406151D0 (en) | 2014-04-04 | 2014-05-21 | Oxford Nanopore Tech Ltd | Method |
| CN105899678A (zh) | 2013-10-18 | 2016-08-24 | 牛津纳米孔技术公司 | 经修饰的酶 |
| AU2015208919B9 (en) | 2014-01-22 | 2021-04-01 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| CN106460061B (zh) | 2014-04-04 | 2020-03-06 | 牛津纳米孔技术公司 | 在双链核酸分子的两端使用纳米孔和锚分子来表征所述双链核酸的方法 |
| WO2015166275A1 (en) | 2014-05-02 | 2015-11-05 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201417712D0 (en) | 2014-10-07 | 2014-11-19 | Oxford Nanopore Tech Ltd | Method |
| KR20250130441A (ko) | 2014-09-01 | 2025-09-01 | 브이아이비 브이지더블유 | 돌연변이체 csgg 포어 |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| CN113981055A (zh) | 2014-10-17 | 2022-01-28 | 牛津纳米孔技术公司 | 纳米孔rna表征方法 |
| WO2017149318A1 (en) | 2016-03-02 | 2017-09-08 | Oxford Nanopore Technologies Limited | Mutant pores |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| EP3464616B1 (en) | 2016-05-25 | 2022-05-04 | Oxford Nanopore Technologies plc | Method |
| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| GB201620450D0 (en) | 2016-12-01 | 2017-01-18 | Oxford Nanopore Tech Ltd | Method |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN117106037B (zh) | 2017-06-30 | 2025-07-25 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010004265A1 (en) * | 2008-07-07 | 2010-01-14 | Oxford Nanopore Technologies Limited | Enzyme-pore constructs |
| WO2010086622A1 (en) * | 2009-01-30 | 2010-08-05 | Oxford Nanopore Technologies Limited | Adaptors for nucleic acid constructs in transmembrane sequencing |
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