CN112805393A - 一种解旋酶及其应用 - Google Patents

一种解旋酶及其应用 Download PDF

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Publication number
CN112805393A
CN112805393A CN201880095718.XA CN201880095718A CN112805393A CN 112805393 A CN112805393 A CN 112805393A CN 201880095718 A CN201880095718 A CN 201880095718A CN 112805393 A CN112805393 A CN 112805393A
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CN
China
Prior art keywords
helicase
pore
target polynucleotide
complex structure
seq
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Pending
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CN201880095718.XA
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English (en)
Inventor
陈呈尧
王慕旸
周雅
付童
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Qitan Technology Ltd Beijing
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Qitan Technology Ltd Beijing
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Application filed by Qitan Technology Ltd Beijing filed Critical Qitan Technology Ltd Beijing
Publication of CN112805393A publication Critical patent/CN112805393A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

Abstract

提供了一种表征目标多核苷酸的方法，及一种F8813解旋酶、一种包含解旋酶的复合体结构及其在表征目标多核苷酸或控制目标多核苷酸穿过孔的运动中的应用。所述的F8813解旋酶或复合体结构具有极强的耐盐性，其用于表征目标多核苷酸的方法可以提高检测核酸序列的精确度。

Description

PCT国内申请，说明书已公开。

Claims (22)

  1. PCT国内申请，权利要求书已公开。
CN201880095718.XA 2018-09-28 2018-09-28 一种解旋酶及其应用 Pending CN112805393A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2018/108227 WO2020061997A1 (zh) 2018-09-28 2018-09-28 一种解旋酶及其应用

Publications (1)

Publication Number Publication Date
CN112805393A true CN112805393A (zh) 2021-05-14

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ID=69950930

Family Applications (1)

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CN201880095718.XA Pending CN112805393A (zh) 2018-09-28 2018-09-28 一种解旋酶及其应用

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CN (1) CN112805393A (zh)
WO (1) WO2020061997A1 (zh)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114230644A (zh) * 2021-12-29 2022-03-25 南京巨匠生物科技有限公司 一种gp32蛋白突变体、重组载体及其构建方法和应用
WO2023123359A1 (zh) * 2021-12-31 2023-07-06 深圳华大生命科学研究院 解旋酶bch2x及其用途
WO2023123347A1 (zh) * 2021-12-31 2023-07-06 深圳华大生命科学研究院 解旋酶bch1x及其用途

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022126304A1 (zh) * 2020-12-14 2022-06-23 北京齐碳科技有限公司 一种经修饰的解旋酶及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985863A (en) * 1996-09-12 1999-11-16 Vertex Pharmaceuticals, Inc. Compositions and methods for decreasing IGIF and IFN-γ production by administering an ICE inhibitor
WO2014013260A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
CN106574300A (zh) * 2014-05-02 2017-04-19 牛津纳米孔技术公司 改善目标多核苷酸相对于跨膜孔移动的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140090633A (ko) * 2011-10-21 2014-07-17 옥스포드 나노포어 테크놀로지즈 리미티드 포어 및 hel308 헬리카제를 사용하여 표적 폴리뉴클레오티드를 특성화하는 방법
CN104136631B (zh) * 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5985863A (en) * 1996-09-12 1999-11-16 Vertex Pharmaceuticals, Inc. Compositions and methods for decreasing IGIF and IFN-γ production by administering an ICE inhibitor
WO2014013260A1 (en) * 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
CN106574300A (zh) * 2014-05-02 2017-04-19 牛津纳米孔技术公司 改善目标多核苷酸相对于跨膜孔移动的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GENBANK登录号：WP_048166958.1: "GenBank登录号：WP_048166958.1", 《GENBANK》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114230644A (zh) * 2021-12-29 2022-03-25 南京巨匠生物科技有限公司 一种gp32蛋白突变体、重组载体及其构建方法和应用
WO2023123359A1 (zh) * 2021-12-31 2023-07-06 深圳华大生命科学研究院 解旋酶bch2x及其用途
WO2023123347A1 (zh) * 2021-12-31 2023-07-06 深圳华大生命科学研究院 解旋酶bch1x及其用途

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Application publication date: 20210514