JP2014526262A5 - - Google Patents
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- Publication number
- JP2014526262A5 JP2014526262A5 JP2014530656A JP2014530656A JP2014526262A5 JP 2014526262 A5 JP2014526262 A5 JP 2014526262A5 JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014526262 A5 JP2014526262 A5 JP 2014526262A5
- Authority
- JP
- Japan
- Prior art keywords
- activity
- fatty acid
- polypeptide
- microbial cell
- recombinant microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000000813 microbial effect Effects 0.000 claims description 102
- 230000000694 effects Effects 0.000 claims description 98
- 229920001184 polypeptide Polymers 0.000 claims description 58
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 58
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 58
- 239000002157 polynucleotide Substances 0.000 claims description 50
- 102000040430 polynucleotide Human genes 0.000 claims description 50
- 108091033319 polynucleotide Proteins 0.000 claims description 50
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 49
- 229930195729 fatty acid Natural products 0.000 claims description 49
- 239000000194 fatty acid Substances 0.000 claims description 49
- 150000004665 fatty acids Chemical class 0.000 claims description 49
- 229940053200 antiepileptics fatty acid derivative Drugs 0.000 claims description 27
- QAQREVBBADEHPA-IEXPHMLFSA-N propionyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 QAQREVBBADEHPA-IEXPHMLFSA-N 0.000 claims description 23
- 102000004190 Enzymes Human genes 0.000 claims description 16
- 108090000790 Enzymes Proteins 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 15
- 230000014509 gene expression Effects 0.000 claims description 14
- 150000002191 fatty alcohols Chemical class 0.000 claims description 12
- 150000002192 fatty aldehydes Chemical class 0.000 claims description 12
- 108010019608 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase Proteins 0.000 claims description 11
- 102100037149 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial Human genes 0.000 claims description 11
- 150000002194 fatty esters Chemical class 0.000 claims description 10
- 229930195733 hydrocarbon Natural products 0.000 claims description 10
- 150000002430 hydrocarbons Chemical class 0.000 claims description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 8
- 108010064711 Homoserine dehydrogenase Proteins 0.000 claims description 8
- 229910052799 carbon Inorganic materials 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 230000001851 biosynthetic effect Effects 0.000 claims description 6
- 150000001336 alkenes Chemical class 0.000 claims description 5
- 238000004519 manufacturing process Methods 0.000 claims description 5
- 108010028984 3-isopropylmalate dehydratase Proteins 0.000 claims description 4
- 108010039636 3-isopropylmalate dehydrogenase Proteins 0.000 claims description 4
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 4
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 4
- 108010055400 Aspartate kinase Proteins 0.000 claims description 4
- 239000004215 Carbon black (E152) Substances 0.000 claims description 4
- 102100029106 Ethylmalonyl-CoA decarboxylase Human genes 0.000 claims description 4
- 108010085747 Methylmalonyl-CoA Decarboxylase Proteins 0.000 claims description 4
- 108010051679 Methylmalonyl-CoA carboxytransferase Proteins 0.000 claims description 4
- 102000005488 Thioesterase Human genes 0.000 claims description 4
- 108010006873 Threonine Dehydratase Proteins 0.000 claims description 4
- 102000006843 Threonine synthase Human genes 0.000 claims description 4
- 108010022394 Threonine synthase Proteins 0.000 claims description 4
- 102100033451 Thyroid hormone receptor beta Human genes 0.000 claims description 4
- 230000002238 attenuated effect Effects 0.000 claims description 4
- 230000002255 enzymatic effect Effects 0.000 claims description 4
- 150000002148 esters Chemical class 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 108010071598 homoserine kinase Proteins 0.000 claims description 4
- 108020002982 thioesterase Proteins 0.000 claims description 4
- 108010063585 (R)-citramalate synthase Proteins 0.000 claims description 3
- 102000019010 Methylmalonyl-CoA Mutase Human genes 0.000 claims description 3
- 108010051862 Methylmalonyl-CoA mutase Proteins 0.000 claims description 3
- 108010082340 Arginine deiminase Proteins 0.000 claims description 2
- 108090000489 Carboxy-Lyases Proteins 0.000 claims description 2
- 102000004031 Carboxy-Lyases Human genes 0.000 claims description 2
- 108030002325 Carboxylate reductases Proteins 0.000 claims description 2
- 101710129019 Long-chain acyl-[acyl-carrier-protein] reductase Proteins 0.000 claims description 2
- 102000030503 Methylmalonyl-CoA epimerase Human genes 0.000 claims description 2
- 241000795633 Olea <sea slug> Species 0.000 claims description 2
- 150000001335 aliphatic alkanes Chemical class 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 108090001018 hexadecanal dehydrogenase (acylating) Proteins 0.000 claims description 2
- 150000002576 ketones Chemical class 0.000 claims description 2
- 108091000124 methylmalonyl-CoA epimerase Proteins 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 230000002018 overexpression Effects 0.000 claims description 2
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 claims 2
- 239000013256 coordination polymer Substances 0.000 claims 1
- XFTRTWQBIOMVPK-UHFFFAOYSA-N (-)-(R)-2-hydroxy-2-methylbutanedioic acid Natural products OC(=O)C(O)(C)CC(O)=O XFTRTWQBIOMVPK-UHFFFAOYSA-N 0.000 description 1
- GGQQNYXPYWCUHG-RMTFUQJTSA-N (3e,6e)-deca-3,6-diene Chemical compound CCC\C=C\C\C=C\CC GGQQNYXPYWCUHG-RMTFUQJTSA-N 0.000 description 1
- XFTRTWQBIOMVPK-RXMQYKEDSA-N D-citramalic acid Chemical compound OC(=O)[C@@](O)(C)CC(O)=O XFTRTWQBIOMVPK-RXMQYKEDSA-N 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38308610P | 2010-09-15 | 2010-09-15 | |
| US13/232,927 | 2011-09-14 | ||
| US13/232,927 US8372610B2 (en) | 2010-09-15 | 2011-09-14 | Production of odd chain fatty acid derivatives in recombinant microbial cells |
| PCT/US2012/028256 WO2013039563A1 (en) | 2010-09-15 | 2012-03-08 | Production of odd chain fatty acid derivatives in recombinant microbial cells |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017011738A Division JP6609274B2 (ja) | 2011-09-14 | 2017-01-26 | 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2014526262A JP2014526262A (ja) | 2014-10-06 |
| JP2014526262A5 true JP2014526262A5 (enExample) | 2015-03-12 |
| JP6141282B2 JP6141282B2 (ja) | 2017-06-07 |
Family
ID=47884064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014530656A Expired - Fee Related JP6141282B2 (ja) | 2010-09-15 | 2012-03-08 | 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US8372610B2 (enExample) |
| EP (2) | EP3957736B1 (enExample) |
| JP (1) | JP6141282B2 (enExample) |
| CN (1) | CN103906845B (enExample) |
| CA (1) | CA2848253C (enExample) |
| MY (1) | MY191370A (enExample) |
| WO (1) | WO2013039563A1 (enExample) |
Families Citing this family (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011522525A (ja) | 2008-05-16 | 2011-08-04 | エルエス９・インコーポレイテッド | 炭化水素を製造するための方法および組成物 |
| WO2010062480A2 (en) | 2008-10-28 | 2010-06-03 | Ls9, Inc. | Methods and compositions for producing fatty alcohols |
| US9957534B2 (en) | 2008-11-06 | 2018-05-01 | Kiverdi, Inc. | Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
| WO2013148348A1 (en) * | 2012-03-28 | 2013-10-03 | Kiverdi, Inc. | Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same |
| JP2013505727A (ja) | 2009-09-27 | 2013-02-21 | オーピーエックス バイオテクノロジーズ， インコーポレイテッド | ３−ヒドロキシプロピオン酸および他の生成物を生成するための方法 |
| MX379251B (es) * | 2012-04-02 | 2025-03-11 | Genomatica Inc | Producción mejorada de derivados de ácidos grasos. |
| EP2882843A4 (en) | 2012-08-10 | 2016-04-06 | Opx Biotechnologies Inc | MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS |
| US20160002681A1 (en) | 2012-12-12 | 2016-01-07 | REG Life Sciences, LLC | Acp-mediated production of fatty acid derivatives |
| US20140193867A1 (en) * | 2012-12-21 | 2014-07-10 | William Marsh Rice University | Microbial odd chain fatty acids |
| KR101746040B1 (ko) | 2013-01-16 | 2017-06-13 | 알이지 라이프 사이언시스, 엘엘씨 | 개선된 특성들을 갖는 아실-ａｃｐ 레덕타아제 |
| CA2902279C (en) | 2013-03-05 | 2019-05-28 | The Procter & Gamble Company | Mixed sugar amine or sugar amide surfactant compositions |
| US20150057465A1 (en) | 2013-03-15 | 2015-02-26 | Opx Biotechnologies, Inc. | Control of growth-induction-production phases |
| AU2014277874B2 (en) | 2013-06-14 | 2020-12-03 | Genomatica, Inc. | Methods of producing omega-hydroxylated fatty acid derivatives |
| US11408013B2 (en) | 2013-07-19 | 2022-08-09 | Cargill, Incorporated | Microorganisms and methods for the production of fatty acids and fatty acid derived products |
| CN106795483A (zh) * | 2013-07-19 | 2017-05-31 | 嘉吉公司 | 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法 |
| US9868682B2 (en) | 2013-08-19 | 2018-01-16 | REG Life Sciences, LLC | Production of partially refined waste glycerol |
| US9469586B2 (en) | 2013-08-19 | 2016-10-18 | REG Life Sciences, LLC | Production of partially refined waste glycerol |
| CA2923730C (en) | 2013-09-13 | 2023-03-14 | REG Life Sciences, LLC | Improved acetyl-coa carboxylase variants |
| US20160376600A1 (en) | 2013-11-25 | 2016-12-29 | Genomatica, Inc. | Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol |
| CA3114049C (en) | 2013-12-05 | 2023-06-13 | REG Life Sciences, LLC | Microbial production of fatty amines |
| US9951356B2 (en) * | 2013-12-12 | 2018-04-24 | Dow Global Technologies Llc | Processes to prepare elongated 2-ketoacids and C6-C10 compounds therefrom via genetic modifications to microbial metabolic pathways |
| CA2944558A1 (en) * | 2014-04-10 | 2015-10-15 | REG Life Sciences, LLC | Semisynthetic routes to organic compounds |
| MX372803B (es) | 2014-06-16 | 2020-07-03 | Genomatica Inc | Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas. |
| US20170152531A1 (en) * | 2014-06-16 | 2017-06-01 | North America S.A.R.L. | Process for producing glutarate and glutaric acid methyl ester |
| CN111748511A (zh) | 2014-07-18 | 2020-10-09 | 基因组股份公司 | 微生物的脂肪二醇产生 |
| EP2993228B1 (en) | 2014-09-02 | 2019-10-09 | Cargill, Incorporated | Production of fatty acid esters |
| WO2016094604A1 (en) | 2014-12-10 | 2016-06-16 | Dow Global Technologies Llc | Genetically modified phenylpyruvate decarboxylase, processes to prepare, and uses thereof |
| US10851393B2 (en) * | 2015-02-24 | 2020-12-01 | William Marsh Rice University | KAS-III free FA synthesis |
| JP6681625B2 (ja) * | 2015-06-16 | 2020-04-15 | 国立大学法人名古屋大学 | タンパク質の発現方法 |
| WO2017101987A1 (en) | 2015-12-15 | 2017-06-22 | REG Life Sciences, LLC | Omega-hydroxylase-related fusion polypeptide variants with improved properties |
| CN106916856B (zh) * | 2015-12-28 | 2021-08-24 | 丰益(上海)生物技术研发中心有限公司 | 提高产脂微生物生产奇数碳脂肪酸产量的培养基及方法 |
| EP3795576B1 (en) | 2016-09-14 | 2023-06-07 | Genomatica, Inc. | 1,3-fatty diol compounds and glycosylated derivatives thereof |
| CA3038660A1 (en) | 2016-09-30 | 2018-04-05 | Dow Global Technologies Llc | Processes to prepare elongated 2-ketoacids and c5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways |
| WO2018144701A2 (en) | 2017-02-02 | 2018-08-09 | Cargill Incorporated | Genetically modified cells that produce c6-c10 fatty acid derivatives |
| BR112019020784A2 (pt) | 2017-04-03 | 2020-04-28 | Genomatica, Inc. | variantes de tioesterase com atividade melhorada para a produção de derivados de ácidos graxos de cadeia média |
| WO2019056071A1 (en) * | 2017-09-21 | 2019-03-28 | The University Of Queensland | RECOMBINANT MICROORGANISMS |
| US11230705B2 (en) | 2017-09-29 | 2022-01-25 | Dow Global Technologies Llc | Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C5-C10 compounds therewith |
| WO2020047304A1 (en) | 2018-08-31 | 2020-03-05 | Genomatica, Inc. | Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose |
| EP3861122A1 (en) * | 2018-10-04 | 2021-08-11 | Anna University | Production of l-2-aminobutyrate from citramalate,citraconate or 2-oxobutanoate |
| CN109517811B (zh) * | 2018-11-29 | 2022-11-11 | 滨州医学院 | 一种β-酮脂酰-ACP合成酶突变体 |
| CN110129400B (zh) * | 2019-05-25 | 2023-09-01 | 华南理工大学 | 一种提高微生物油脂奇数碳链脂肪酸含量的方法 |
| CN112941096B (zh) * | 2019-12-10 | 2023-05-26 | 北京化工大学 | 重组质粒组合、基因改造酵母菌及生产奇数链脂肪酸方法 |
| WO2023178197A1 (en) | 2022-03-16 | 2023-09-21 | Genomatica, Inc. | Recombinant microbes for production of trans-2 unsaturated fatty acids and derivatives thereof |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US6428767B1 (en) | 1995-05-12 | 2002-08-06 | E. I. Du Pont De Nemours And Company | Method for identifying the source of carbon in 1,3-propanediol |
| CN101490241A (zh) * | 2006-05-19 | 2009-07-22 | Ls9公司 | 脂肪酸及其衍生物的制备 |
| US8110670B2 (en) * | 2006-05-19 | 2012-02-07 | Ls9, Inc. | Enhanced production of fatty acid derivatives |
| AU2007254151A1 (en) | 2006-05-19 | 2007-11-29 | Ls9, Inc. | Production of fatty acids and derivatives thereof |
| EP2118266A4 (en) | 2007-02-09 | 2010-05-19 | Univ California | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS |
| US9695426B2 (en) | 2007-02-09 | 2017-07-04 | The Regents Of The University Of California | Biofuel production by recombinant microorganisms |
| CA3035878C (en) | 2007-03-28 | 2023-01-03 | REG Life Sciences, LLC | Enhanced production of fatty acid derivatives |
| JP5912251B2 (ja) | 2007-05-22 | 2016-06-01 | アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー | 炭化水素産生遺伝子およびその使用方法 |
| EP2327769B1 (en) * | 2007-11-10 | 2013-03-27 | Joule Unlimited Technologies, Inc. | Hyperphotosynthetic organisms |
| US8183028B2 (en) | 2007-12-21 | 2012-05-22 | Ls9, Inc. | Methods and compositions for producing olefins |
| JP2011522525A (ja) | 2008-05-16 | 2011-08-04 | エルエス９・インコーポレイテッド | 炭化水素を製造するための方法および組成物 |
| CA3038491A1 (en) | 2008-10-07 | 2010-04-15 | REG Life Sciences, LLC | Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase |
| WO2010062480A2 (en) | 2008-10-28 | 2010-06-03 | Ls9, Inc. | Methods and compositions for producing fatty alcohols |
| EP3564345A1 (en) | 2008-12-23 | 2019-11-06 | REG Life Sciences, LLC | Methods and compositions related to thioesterase enzymes |
| WO2010135624A2 (en) * | 2009-05-22 | 2010-11-25 | Codexis, Inc. | Engineered biosynthesis of fatty alcohols |
| EP2475775A4 (en) | 2009-09-09 | 2013-03-20 | Genomatica Inc | MICROORGANISMS AND METHOD FOR THE SIMULTANEOUS PREPARATION OF ISOPROPANOL AND PRIMARY ALCOHOLS, DIOLES AND ACIDS |
| US20110166370A1 (en) | 2010-01-12 | 2011-07-07 | Charles Winston Saunders | Scattered Branched-Chain Fatty Acids And Biological Production Thereof |
-
2011
- 2011-09-14 US US13/232,927 patent/US8372610B2/en not_active Expired - Fee Related
-
2012
- 2012-03-08 WO PCT/US2012/028256 patent/WO2013039563A1/en not_active Ceased
- 2012-03-08 US US14/344,658 patent/US20150167033A1/en not_active Abandoned
- 2012-03-08 CA CA2848253A patent/CA2848253C/en active Active
- 2012-03-08 EP EP21177335.3A patent/EP3957736B1/en active Active
- 2012-03-08 EP EP12709480.3A patent/EP2756090B1/en active Active
- 2012-03-08 CN CN201280054084.6A patent/CN103906845B/zh active Active
- 2012-03-08 MY MYPI2014000550A patent/MY191370A/en unknown
- 2012-03-08 JP JP2014530656A patent/JP6141282B2/ja not_active Expired - Fee Related
-
2021
- 2021-02-08 US US17/169,845 patent/US20210324431A1/en active Pending
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