JP6141282B2 - 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 - Google Patents

組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 Download PDF

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JP6141282B2
JP6141282B2 JP2014530656A JP2014530656A JP6141282B2 JP 6141282 B2 JP6141282 B2 JP 6141282B2 JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014530656 A JP2014530656 A JP 2014530656A JP 6141282 B2 JP6141282 B2 JP 6141282B2
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polypeptide
fatty acid
activity
coa
coli
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JP2014526262A5 (enExample
JP2014526262A (ja
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グレイス・ジェイ・リー
ジョン・アール・ハリバートン
フ・ジーハオ
アンドレアス・ヴェー・シルマー
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アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
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JP2014530656A 2010-09-15 2012-03-08 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 Expired - Fee Related JP6141282B2 (ja)

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US38308610P 2010-09-15 2010-09-15
US13/232,927 2011-09-14
US13/232,927 US8372610B2 (en) 2010-09-15 2011-09-14 Production of odd chain fatty acid derivatives in recombinant microbial cells
PCT/US2012/028256 WO2013039563A1 (en) 2010-09-15 2012-03-08 Production of odd chain fatty acid derivatives in recombinant microbial cells

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Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010012192A (es) 2008-05-16 2011-04-26 Ls9 Inc Metodos y composiciones para producir hidrocarburos.
EP2796559B1 (en) 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
WO2013148348A1 (en) * 2012-03-28 2013-10-03 Kiverdi, Inc. Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
US9957534B2 (en) 2008-11-06 2018-05-01 Kiverdi, Inc. Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
WO2011038364A1 (en) 2009-09-27 2011-03-31 Opx Biotechnologies, Inc. Method for producing 3-hydroxypropionic acid and other products
BR122019026142B1 (pt) * 2012-04-02 2021-12-14 Genomatica, Inc Célula hospedeira recombinante
BR112015002940A2 (pt) 2012-08-10 2018-04-24 Opx Biotechnologies Inc microorganismos e métodos para a produção de ácidos graxos e produtos derivados de ácidos graxos.
WO2014093505A2 (en) 2012-12-12 2014-06-19 Ls9, Inc. Acp-mediated production of fatty acid derivatives
US20140193867A1 (en) * 2012-12-21 2014-07-10 William Marsh Rice University Microbial odd chain fatty acids
CN110387352A (zh) 2013-01-16 2019-10-29 Reg生命科学有限责任公司 具有改善特性的酰基-acp还原酶
JP6251296B2 (ja) 2013-03-05 2017-12-20 ザ プロクター アンド ギャンブル カンパニー 混合糖組成物
US9447438B2 (en) 2013-03-15 2016-09-20 Cargill, Incorporated Acetyl-coA carboxylases
KR20220053681A (ko) 2013-06-14 2022-04-29 게노마티카 인코포레이티드 오메가-수산화 지방산 유도체를 생성하는 방법들
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
WO2015010103A2 (en) * 2013-07-19 2015-01-22 Opx Biotechnologies, Inc. Microorganisms and methods for the production of fatty acids and fatty acid derived products
KR20160060643A (ko) 2013-08-19 2016-05-30 알이지 라이프 사이언시스, 엘엘씨 부분 정제된 폐글리세롤의 생성
US9469586B2 (en) 2013-08-19 2016-10-18 REG Life Sciences, LLC Production of partially refined waste glycerol
EP2964756B1 (en) 2013-09-13 2017-05-03 REG Life Sciences, LLC Improved acetyl-coa carboxylase variants
US20160376600A1 (en) 2013-11-25 2016-12-29 Genomatica, Inc. Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol
WO2015085271A1 (en) 2013-12-05 2015-06-11 REG Life Sciences, LLC Microbial production of fatty amines
CA2933470C (en) * 2013-12-12 2023-10-03 Dow Global Technologies Llc Processes to prepare elongated 2-ketoacids and c6-c10 compounds therefrom via genetic modifications to microbial metabolic pathways
CA2944558A1 (en) 2014-04-10 2015-10-15 REG Life Sciences, LLC Semisynthetic routes to organic compounds
WO2015195697A1 (en) 2014-06-16 2015-12-23 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptides with improved properties
CN106795519A (zh) * 2014-06-16 2017-05-31 英威达技术有限责任公司 用于生成戊二酸和戊二酸甲酯的方法
CN106574238B (zh) 2014-07-18 2021-09-21 基因组股份公司 微生物的脂肪二醇产生
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
ES2792853T3 (es) 2014-12-10 2020-11-12 Dow Global Technologies Llc Fenilpiruvato descarboxilasa modificada genéticamente, procedimientos de preparación y usos de la misma
WO2016137897A1 (en) * 2015-02-24 2016-09-01 William Marsh Rice University Kasiii-free fa synthesis
JP6681625B2 (ja) * 2015-06-16 2020-04-15 国立大学法人名古屋大学 タンパク質の発現方法
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
CN106916856B (zh) * 2015-12-28 2021-08-24 丰益(上海)生物技术研发中心有限公司 提高产脂微生物生产奇数碳脂肪酸产量的培养基及方法
KR102456406B1 (ko) 2016-09-14 2022-10-19 게노마티카 인코포레이티드 1,3-지방 디올 화합물 및 그 유도체
CA3038662A1 (en) 2016-09-30 2018-04-05 Dow Global Technologies Llc Processes to prepare elongated 2-ketoacids and c5-c10 compounds therefr om via genetic modifications to microbial metabolic pathways
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
KR102677413B1 (ko) 2017-04-03 2024-06-24 게노마티카 인코포레이티드 중쇄 지방산 유도체의 생산을 위해 개선된 활성을 갖는 티오에스테라제 변이체
WO2019056071A1 (en) * 2017-09-21 2019-03-28 The University Of Queensland RECOMBINANT MICROORGANISMS
US11230705B2 (en) 2017-09-29 2022-01-25 Dow Global Technologies Llc Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C5-C10 compounds therewith
EP3844179A4 (en) 2018-08-31 2022-08-31 Genomatica, Inc. XYLR MUTANT FOR IMPROVED XYLOSE UTILIZATION OR IMPROVED CO-UTILIZATION OF GLUCOSE AND XYLOSE
JP7601774B2 (ja) * 2018-10-04 2024-12-17 アンナ・ユニバーシティ シトラマル酸、シトラコン酸又は２－オキソ酪酸からのｌ－２－アミノ酪酸の生成
CN109517811B (zh) * 2018-11-29 2022-11-11 滨州医学院 一种β-酮脂酰-ACP合成酶突变体
CN110129400B (zh) * 2019-05-25 2023-09-01 华南理工大学 一种提高微生物油脂奇数碳链脂肪酸含量的方法
CN112941096B (zh) * 2019-12-10 2023-05-26 北京化工大学 重组质粒组合、基因改造酵母菌及生产奇数链脂肪酸方法
WO2023178197A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Recombinant microbes for production of trans-2 unsaturated fatty acids and derivatives thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7791991A (en) 1990-04-24 1991-11-11 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
JP5933909B2 (ja) 2006-05-19 2016-06-15 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 脂肪酸およびその誘導体の生産
US8110670B2 (en) * 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
US9695426B2 (en) 2007-02-09 2017-07-04 The Regents Of The University Of California Biofuel production by recombinant microorganisms
CA2678261A1 (en) * 2007-02-09 2008-08-14 The Regents Of The University Of California Biofuel production by recombinant microorganisms
CA3178593A1 (en) 2007-03-28 2008-10-02 Genomatica, Inc. Enhanced production of fatty acid derivatives
BRPI0813359A2 (pt) 2007-05-22 2014-10-14 Ls9 Inc Ácidos nucleicos isolados, vetor, células, métodos para produzir um hidrocarboneto, para produzir uma cetona alifática, para produzir um polipeptídeo purificado, para produzir um hidrocarboneto, para produzir biocombustíveis, hidrocarboneto, biocombustível, polipeptídeo isolado, ácido nucleico isolado, organismos geneticamente engenheirados, método para identificar uma enzima útil para a produção de hidrocarbonetos
CA2704227A1 (en) * 2007-11-10 2009-05-14 Joule Biotechnologies, Inc. Hyperphotosynthetic organisms
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
MX2010012192A (es) 2008-05-16 2011-04-26 Ls9 Inc Metodos y composiciones para producir hidrocarburos.
MX2011003597A (es) 2008-10-07 2011-06-20 Ls9 Inc Metodos y composiciones para producir aldehidos grasos.
EP2796559B1 (en) 2008-10-28 2018-09-12 REG Life Sciences, LLC Microbial cell for producing fatty alcohols
CN110129296A (zh) 2008-12-23 2019-08-16 Reg生命科学有限责任公司 硫酯酶相关的方法和组合物
WO2010135624A2 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols
BR112012005296A2 (pt) 2009-09-09 2019-01-15 Genomatica Inc microorganismos e métodos para a coprodução de isopropanol com alcoóis primários, diós e ácidos.
US8933131B2 (en) 2010-01-12 2015-01-13 The Procter & Gamble Company Intermediates and surfactants useful in household cleaning and personal care compositions, and methods of making the same

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