JP6141282B2 - 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 - Google Patents

組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 Download PDF

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JP6141282B2
JP6141282B2 JP2014530656A JP2014530656A JP6141282B2 JP 6141282 B2 JP6141282 B2 JP 6141282B2 JP 2014530656 A JP2014530656 A JP 2014530656A JP 2014530656 A JP2014530656 A JP 2014530656A JP 6141282 B2 JP6141282 B2 JP 6141282B2
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polypeptide
fatty acid
activity
coa
coli
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JP2014526262A (ja
JP2014526262A5 (enExample
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グレイス・ジェイ・リー
ジョン・アール・ハリバートン
フ・ジーハオ
アンドレアス・ヴェー・シルマー
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アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー
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JP2014530656A 2010-09-15 2012-03-08 組換え微生物細胞における奇数鎖脂肪酸誘導体の生成 Expired - Fee Related JP6141282B2 (ja)

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US38308610P 2010-09-15 2010-09-15
US13/232,927 2011-09-14
US13/232,927 US8372610B2 (en) 2010-09-15 2011-09-14 Production of odd chain fatty acid derivatives in recombinant microbial cells
PCT/US2012/028256 WO2013039563A1 (en) 2010-09-15 2012-03-08 Production of odd chain fatty acid derivatives in recombinant microbial cells

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JP2014526262A5 JP2014526262A5 (enExample) 2015-03-12
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Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011522525A (ja) 2008-05-16 2011-08-04 エルエス９・インコーポレイテッド 炭化水素を製造するための方法および組成物
WO2010062480A2 (en) 2008-10-28 2010-06-03 Ls9, Inc. Methods and compositions for producing fatty alcohols
US9957534B2 (en) 2008-11-06 2018-05-01 Kiverdi, Inc. Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
WO2013148348A1 (en) * 2012-03-28 2013-10-03 Kiverdi, Inc. Engineered co2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
JP2013505727A (ja) 2009-09-27 2013-02-21 オーピーエックス バイオテクノロジーズ， インコーポレイテッド ３−ヒドロキシプロピオン酸および他の生成物を生成するための方法
MX379251B (es) * 2012-04-02 2025-03-11 Genomatica Inc Producción mejorada de derivados de ácidos grasos.
EP2882843A4 (en) 2012-08-10 2016-04-06 Opx Biotechnologies Inc MICRO-ORGANISMS AND METHOD FOR THE PRODUCTION OF FATTY ACIDS AND PRODUCTS DERIVED FROM FATTY ACIDS
US20160002681A1 (en) 2012-12-12 2016-01-07 REG Life Sciences, LLC Acp-mediated production of fatty acid derivatives
US20140193867A1 (en) * 2012-12-21 2014-07-10 William Marsh Rice University Microbial odd chain fatty acids
KR101746040B1 (ko) 2013-01-16 2017-06-13 알이지 라이프 사이언시스, 엘엘씨 개선된 특성들을 갖는 아실-ａｃｐ 레덕타아제
CA2902279C (en) 2013-03-05 2019-05-28 The Procter & Gamble Company Mixed sugar amine or sugar amide surfactant compositions
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
AU2014277874B2 (en) 2013-06-14 2020-12-03 Genomatica, Inc. Methods of producing omega-hydroxylated fatty acid derivatives
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
CN106795483A (zh) * 2013-07-19 2017-05-31 嘉吉公司 用于生产脂肪酸和脂肪酸衍生产物的微生物及方法
US9868682B2 (en) 2013-08-19 2018-01-16 REG Life Sciences, LLC Production of partially refined waste glycerol
US9469586B2 (en) 2013-08-19 2016-10-18 REG Life Sciences, LLC Production of partially refined waste glycerol
CA2923730C (en) 2013-09-13 2023-03-14 REG Life Sciences, LLC Improved acetyl-coa carboxylase variants
US20160376600A1 (en) 2013-11-25 2016-12-29 Genomatica, Inc. Methods for enhancing microbial production of specific length fatty alcohols in the presence of methanol
CA3114049C (en) 2013-12-05 2023-06-13 REG Life Sciences, LLC Microbial production of fatty amines
US9951356B2 (en) * 2013-12-12 2018-04-24 Dow Global Technologies Llc Processes to prepare elongated 2-ketoacids and C6-C10 compounds therefrom via genetic modifications to microbial metabolic pathways
CA2944558A1 (en) * 2014-04-10 2015-10-15 REG Life Sciences, LLC Semisynthetic routes to organic compounds
MX372803B (es) 2014-06-16 2020-07-03 Genomatica Inc Polipeptidos de fusion relacionados con omega-hidroxilasa con propiedades mejoradas.
US20170152531A1 (en) * 2014-06-16 2017-06-01 North America S.A.R.L. Process for producing glutarate and glutaric acid methyl ester
CN111748511A (zh) 2014-07-18 2020-10-09 基因组股份公司 微生物的脂肪二醇产生
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
WO2016094604A1 (en) 2014-12-10 2016-06-16 Dow Global Technologies Llc Genetically modified phenylpyruvate decarboxylase, processes to prepare, and uses thereof
US10851393B2 (en) * 2015-02-24 2020-12-01 William Marsh Rice University KAS-III free FA synthesis
JP6681625B2 (ja) * 2015-06-16 2020-04-15 国立大学法人名古屋大学 タンパク質の発現方法
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
CN106916856B (zh) * 2015-12-28 2021-08-24 丰益(上海)生物技术研发中心有限公司 提高产脂微生物生产奇数碳脂肪酸产量的培养基及方法
EP3795576B1 (en) 2016-09-14 2023-06-07 Genomatica, Inc. 1,3-fatty diol compounds and glycosylated derivatives thereof
CA3038660A1 (en) 2016-09-30 2018-04-05 Dow Global Technologies Llc Processes to prepare elongated 2-ketoacids and c5-c10 compounds therefrom via genetic modifications to microbial metabolic pathways
WO2018144701A2 (en) 2017-02-02 2018-08-09 Cargill Incorporated Genetically modified cells that produce c6-c10 fatty acid derivatives
BR112019020784A2 (pt) 2017-04-03 2020-04-28 Genomatica, Inc. variantes de tioesterase com atividade melhorada para a produção de derivados de ácidos graxos de cadeia média
WO2019056071A1 (en) * 2017-09-21 2019-03-28 The University Of Queensland RECOMBINANT MICROORGANISMS
US11230705B2 (en) 2017-09-29 2022-01-25 Dow Global Technologies Llc Genetically modified isopropylmalate isomerase enzyme complexes and processes to prepare elongated 2-ketoacids and C5-C10 compounds therewith
WO2020047304A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose
EP3861122A1 (en) * 2018-10-04 2021-08-11 Anna University Production of l-2-aminobutyrate from citramalate,citraconate or 2-oxobutanoate
CN109517811B (zh) * 2018-11-29 2022-11-11 滨州医学院 一种β-酮脂酰-ACP合成酶突变体
CN110129400B (zh) * 2019-05-25 2023-09-01 华南理工大学 一种提高微生物油脂奇数碳链脂肪酸含量的方法
CN112941096B (zh) * 2019-12-10 2023-05-26 北京化工大学 重组质粒组合、基因改造酵母菌及生产奇数链脂肪酸方法
WO2023178197A1 (en) 2022-03-16 2023-09-21 Genomatica, Inc. Recombinant microbes for production of trans-2 unsaturated fatty acids and derivatives thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0528881A4 (en) 1990-04-24 1993-05-26 Stratagene Methods for phenotype creation from multiple gene populations
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
CN101490241A (zh) * 2006-05-19 2009-07-22 Ls9公司 脂肪酸及其衍生物的制备
US8110670B2 (en) * 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
AU2007254151A1 (en) 2006-05-19 2007-11-29 Ls9, Inc. Production of fatty acids and derivatives thereof
EP2118266A4 (en) 2007-02-09 2010-05-19 Univ California BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS
US9695426B2 (en) 2007-02-09 2017-07-04 The Regents Of The University Of California Biofuel production by recombinant microorganisms
CA3035878C (en) 2007-03-28 2023-01-03 REG Life Sciences, LLC Enhanced production of fatty acid derivatives
JP5912251B2 (ja) 2007-05-22 2016-06-01 アールイージー ライフ サイエンシズ リミテッド ライアビリティ カンパニー 炭化水素産生遺伝子およびその使用方法
EP2327769B1 (en) * 2007-11-10 2013-03-27 Joule Unlimited Technologies, Inc. Hyperphotosynthetic organisms
US8183028B2 (en) 2007-12-21 2012-05-22 Ls9, Inc. Methods and compositions for producing olefins
JP2011522525A (ja) 2008-05-16 2011-08-04 エルエス９・インコーポレイテッド 炭化水素を製造するための方法および組成物
CA3038491A1 (en) 2008-10-07 2010-04-15 REG Life Sciences, LLC Bacterial host cells engineered to express a carboxylic acid reductase and a thioesterase
WO2010062480A2 (en) 2008-10-28 2010-06-03 Ls9, Inc. Methods and compositions for producing fatty alcohols
EP3564345A1 (en) 2008-12-23 2019-11-06 REG Life Sciences, LLC Methods and compositions related to thioesterase enzymes
WO2010135624A2 (en) * 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols
EP2475775A4 (en) 2009-09-09 2013-03-20 Genomatica Inc MICROORGANISMS AND METHOD FOR THE SIMULTANEOUS PREPARATION OF ISOPROPANOL AND PRIMARY ALCOHOLS, DIOLES AND ACIDS
US20110166370A1 (en) 2010-01-12 2011-07-07 Charles Winston Saunders Scattered Branched-Chain Fatty Acids And Biological Production Thereof

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